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Benzoic Acid in Food Ti Tri Metric Method

Benzoic Acid in Food Ti Tri Metric Method

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Published by: caramel_3000 on May 10, 2011
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05/10/2011

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Determination of Sodium Benzoate From: Official Methods of Analysis of the Association of Official Analytical th Chemists 14 Edition 20.024-20.

025

.0072g anhydrous Na benzoate.Benzoic Acid in Food Titrimetric Method (The presence of Vanillin interferes) 1. 3. neutralize to phenolphthalein. 8. Add approximately ¼ of this volume of H2O and 1 or 2 drops of phenolphthalein 12. If and emulsion forms. The CHCl3 layer usually separates readily after standing for a few minutes. If this precaution is taken the CHCl3 extraction need not be washed.50. 9. 7. 5. then add 5 ml in excess.40 and 30 ml. 11. 10. Neutralize to litmus paper with HCL. Transfer combined CHCl3 extracts to a porcelain evaporating dish. shaking frequently. Thoroughly mix the sample 2. Pipette 100-200 ml of filtrate into separator. Titrate with 0.05N NaOH. carefully drain as much clear CHCl3 solution as possible after each extraction.05 N NaOH = 0. As this is a progressive extraction. or by centrifugation for a few minutes. Dry residue overnight in desiccator. Shake thoroughly and let stand for more than 2 hours. Extract carefully with CHCl3 using successive portions of 70. 1ml 0. rinse container several times with a few mls CHCL3 and evaporate to dryness at room temperature in a current of dry air. break it by stirring CHCl3 layer with glass rod. but do not drain any of the emulsion with CHCl3 layer. Transfer 150 ml or 150 grams to 500ml Volumetric Flask. Make alkaline to litmus paper using 10% NaOH solution or milk of lime and dilute to volume with saturated NaCl solution. Dissolve residue of benzoic acid in 30-50 ml alcohol. Add enough pulverized NaCl to saturate the H2O in the sample 4. Filter 6. Shake cautiously using rotary motion each time to avoid emulsion. by drawing off into a second separator and giving 1 or 2 sharp shakes from one end of the separator to the other.

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