sciFLEX Quick Start

PRE-POWER UP CHECKLIST
Wash bottle filled....................................................................................... CHECK
Waste bottle empty .................................................................................. CHECK
System liquid bottle filled to appropriate level.................................... CHECK
• Use filtered (0,22µm) freshly degased millipore water
• The system liquid surface and the nozzle tip (when in front of the camera station)
!
must be on the same level.

sciFLEXARRAYER stage is clear and free of obstacles ....................... CHECK

All cover hood doors ............................................................................. CLOSED
• The doors must be closed to activate the magnetic door sensors.

Emergency stop switches ....................................................................... ARMED

• The emergency stop switches must be pulled out (one in the S3; two in S5/S11).

POWERING UP
Multi-outlet power strip switch ........................................................ SWITCH ON
"Clunk"-sound of the spotter ................................................................... CHECK
• After ~20 seconds of powering the axes (S5/S11 only), a distinct sound is audible.

Computer ......................................................................................... SWITCH ON

sciFLEX Software ................................................................. START INIT AXES
Software started without error messages ............................................. CHECK

FLUIDIC SETUP (1)

Desired liquid channel(s) .......................................................................... SELECT

• In the sciFLEX-Software click the tab Nozzle Setup. (2)
• Click the green buttons corresponding to the desired channel to activate it (1).

Task "MorningWashProcedure" .......................................................... ACTIVATE

• Click the tab Nozzle Setup and then Do Task (2).

• Select MorningWashProcedure and click DO to start.

• A pop-up window will guide you through the operation (duration 20 mins).

DROP OPTIMIZATION (3)

• In the SNAP WINDOW click: Image > show center to obtain the centered green cross.
• For better nozzle visibility activate the strobe LED by setting a pulse of 1µs and
select Continuous Dispensing and click START (image will go bright).

Nozzle position offset values ...................................................................ADJUST (4)

• In the Nozzle Offset tab, adjust the nozzle position via the arrow buttons
until the nozzle is positioned as shown in (3).
• Click START again to turn off the piezo (Image will go dark).

Nozzle parameter........................................................................................ ENTER

• Set the parameters for Voltage and Pulse you obtained for your nozzle, into
the according fields (4).

• Save the parameters by clicking the Set Nozzle Parameter button.

• Select Continuous Dispensing in the dispense mode window and click START.
(5)
A drop should be visible now.
• Fine tune the parameters until the drop distance is approx. 450 ± 25 µm (5).
450 ± 25µm
OK Not OK
Possible drop shapes:
instable shape satellite position
v1.20 - 03.04.2009