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INCORPORATED

ProSci

Rabbit Polyclonal Antibody Production
Pkg. 1 $700 Anti-Sera Schedule
* * * * * 8 2 2 8 6 week schedule rabbits pre-bleeds (5 ml per animal) immunizations (4 per animal) production bleeds (3 per animal)

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Pkg. 4 $2,835

12170 Flint Place Poway, CA 92064 Tel: 858.513.2638 Fax: 858.513.2692 Toll-Free: 888.513.9525 www.prosci-inc.com abservices@prosci-inc.com

Pkg. 2 $1,335

Pkg. 3 $1,835

Individual Services $700

Peptide Synthesis
* Up to 15 residues for packages 2-4 * Up to 30 residues for custom package * Allow 2 weeks

$20/residue

Conjugation
* To carrier protein to KLH * Allow 1 week

$275

ELISA Report
* On 1st bleed for packages 2-4

$60

Affinity Purification
* 25 ml of serum purified * Includes re-usable column

$500

Large Scale Affinity Purification
* 150 ml of serum purified * Includes re-usable column

$1,500

Protein A or G Purification
* 25 ml of serum purified

$375

Custom Antibody Screening
* Screening panel of ProSci IHC tissues * Screen 13 ProSci Westen Blot lysates

$300 $300 $120

Project Extension (per animal)
* 28 Days * 1 immunization * 2 production bleeds (20-25 ml each)

Project Exsanguination

(per animal)

$75

* Series of 2 bleeds for total of ~60 ml

Example of a 2 rabbit custom antibody schedule:
Week 0 Week 0 Week 2 Week 4 Week 5 Week 6 Week 7 Week 8 (Pre-bleed) (Immunize) (Immunize) (Immunize) (Bleed) (Immunize) (Bleed) (Bleed) Pre-immune serum collection 200 ug/rabbit in Complete Freund's Adjuvant (CFA) 100 ug/rabbit in Incomplete Freund's Adjuvant (IFA) 100 ug/rabbit in Incomplete Freund's Adjuvant (IFA) 1st production bleed 100 ug/rabbit in Incomplete Freund's Adjuvant (IFA) 2nd production bleed 3rd production bleed ~ 5 ml serum/rabbit

090115

20-25 ml serum/rabbit 20-25 ml serum/rabbit 20-25 ml serum/rabbit