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Project Coordinator DBT-UR-IPLS Centre for Converging Technologies University of Rajasthan, Jaipur Sub: Application for Assistant Professor in the DBT-UR-IPLS at Centre for Converging Technologies University of Rajasthan, Jaipur Respected Sir, I am writing to apply for the position of Assistant Professor which was advertised in The Hindu, Dated 09-04-2011. Please find the following documents enclosed with my CV. I do hope that you will get the application in proper manner. Curriculum Vitae Proof of Date of birth (Secondary School Certificate) Senior School Certificate B.Sc. Marksheet M.Sc. Marksheet M.Sc. Provisional Degree Ph.D. Thesis Submission Certificate NET certificate ICMR-JRF certificate GATE 2007 Certificate ARS NET 2010 Certificate RPSC SET 2010 Certificate Madurai Kamraj University Training Course Certificate NBPGR, New Delhi Training Course certificate Certificates of Seminars/Conferences attended/organized Publications in peer reviewed Journals Yours Sincerely

Rohit Jain SRF-CSIR Department of Botany University of Rajasthan, Jaipur

CURRICULUM VITAE Rohit Jain Lab No. 7, Dept. of Botany University of Rajasthan Jaipur (Rajasthan), India-302 004 Email: jainrohit17@gmail.com Mobile: +91 9414040903

Objective: To secure a challenging position in the Biological Science by contributing in Research and Development through hard work, dedication and continuously updating my knowledge by assimilating the latest trends in science with a positive attitude. Current Position: Working as a Senior Research Fellow (CSIR) under the supervision of Prof. S. L. Kothari, Dean Faculty of Science, Department of Botany, University of Rajasthan, Jaipur.

Academic Details: 2007-2011 2005-2007 2002-2005 1999-2000 1997-1998 : : : : : Ph.D. (Botany)-Thesis Submitted (09-02-2011) Master of Science (Botany with specialization in Plant Physiology) from Department of Botany, University of Rajasthan, Jaipur (71.33%) Bachelor of Science (Biology) from University Maharaja’s College, University of Rajasthan, Jaipur (71%) Sr. Secondary (Biology) from Govt. Sr. Hr. Sec. School Lalsot, Dausa, Rajasthan Board of Secondary Education, Ajmer (68.62%) Secondary from Jyoti Secondary School, Lalsot, Dausa, Rajasthan Board of Secondary Education, Ajmer (84.55%)

Awards and Honors:      Qualified National Eligibility Test (NET) for Lectureship (June 2007) Qualified ICMR JRF Test for Project Fellowship (2007) Qualified Graduate Aptitude Test for Engineering (GATE) 2007 – 97 Percentile Qualified RPSC State Eligibility Test (SET) 2010 Qualified ARS NET 2010

Training:  Summer training on “Recombinant DNA Technology (13th - 27th June 2009)” at UGC – Networking Resources Centre in Biological Sciences, Madurai Kamraj University, Madurai.

International workshop on “Cryopreservation and in vitro techniques for conservation of plant genetic resources” (15th – 27th November 2010) at National Bureau for Plant Genetic Resources (NBPGR) IARI, PUSA, New Delhi.

Work Experience Senior Research Fellow in a major research scheme of CSIR entitled “Collection, characterization and conservation of Withania coagulans (Stocks) Dunal chemotypes/biotypes and their metabolomic comparison with Withania somnifera (L.) Dunal counterparts” (3 ½ Years) Achievements:     Successfully developed an efficient micropropagation protocol for ex situ conservation of endangered medicinal plant species Withania coagulans from shoot tip, node and leaf explants Enhancement of withanolides (bioactive compounds of Withania) in in vitro regenerated plants in Withania coagulans Developed a protocol for the synthesis and characterization of silver nanoparticles using spore crystal mixture of Bacillus thuringiensis Isolated and characterised a carotenoid producing novel Actinomycetes strain Gordonia lacunae (NCBI Gene Bank Accession GU727686)

Publications in Peer reviewed Journals (SCI Listed): 1. Jain R, Sinha A, Jain D, Kachhwaha S, Kothari SL Adevntitious shoot regeneration and in vitro biosynthesis of steroidal lactones in W. Coagulans (Stocks) Dunal. Plant Cell Tissue and Organ Culture (2011) 105:135140 (IF: 1.27) 2. Jain D, Kachhwaha S, Jain R, Srivastava G, Kothari SL Novel microbial route to synthesize silver nanoparticles using spore crystal mixture of Bacillus thuringiensis. Indian Journal of Experimental Biology (2010) 48: 1152 – 1156 (IF: 0.55) 3. Sinha A, Jain R, Kachhwaha S, Kothari SL Optimization of the level of micronutrient copper in the culture medium improves shoot bud regeneration in Indian Ginseng [Withania somnifera (L.) Dunal]. National Academy Science Letters (2010) 33: 11-16 (IF: 0.17) 4. Jain R, Sinha A, Kachhwaha S, Kothari SL Micropropagation of Withania coagulans (Stocks) Dunal. : A critically endangered medicinal herb. Journal of Plant Biochemistry and Biotechnology (2009) 18:249252 (IF: 0.41) 5. Jain D, Jain R, Agarwal V, Sharma P, Srivastava G, Kachhwaha S, Kothari SL. Gordonia lacunae strain CCC12 16S ribosomal RNA gene, partial sequence. (2010) NCBI Gene Bank Accession GU727686.

6. Singh-Adhayach P, Arora A, Darji BL, Jain R Pollen grain fertility as a biomonitoring parameter for air pollution. Our Earth (2010) 7: 17-18 7. Jain D, Rathod KS, Jain R, Singh H, Gupta V, Tanwar S, Kachhwaha S, Kothari SL Phytofabrication of iron oxide nanoparticles using Calotropis gigantea L. (Communicated) Digest Journal of Nanomaterials and Biostructures Abstracts/Poster Presented: 1. Jain D, Jain R, Kachhwaha S, Kothari SL, (2010) Molecular Characterization and PCR based detection of cry genes in native Bacillus thuringiensis strains isolated from the desert soils of Rajasthan in Indo-US international workshop on “Plant genomics in crop improvement with special reference to biotic and abiotic stress ” at CCS Haryana Agriculture University, Hisar. 2. Jain D, Jain R, Kachhwaha S, Kothari SL (2009) Effect of Ramp rate on RAPD analysis: An important step in optimization and reproducibility of RAPD in National Workshop at Jaipur National University, Jaipur.

Experimental Expertise  Molecular Biology : Plasmid isolation, Bacterial transformation, Restriction Enzyme digestion and ligation, Isolation of Genomic DNA, Isolation of RNA, cDNA preparation, SDS-PAGE, Elution of proteins, Construction and Screening of library, Southern and Western Blot analysis, Genetic Transformation by Biolistic Gene Gun (PDS 1000), Expression and purification of recombinant proteins  PCR techniques: Designing of PCR primers, Cloning of PCR products, Gradient PCR and RT-PCR  Biochemical Analysis: Extraction of Primary and Secondary metabolites, Thin Layer Chromatography, Column Chromatography, Liquid-liquid Partition Chromatography, Reverse Phase-HPLC, UV-Visible and Nano Drop Spectrophotometry  Bioinformatics: ClustalW, Sequence analysis using BLAST and EMBL tools, BIOEDIT, NTSYS, FASTPCR, NETPRIMER  Tissue culture techniques: Tissue culture of Withania Spp., Agrobacterium mediated transformation in Withania  Nanotechnology: Nanomaterial synthesis through biological route, Characterization of nanomaterials using XRD, SEM-TEM

Shailesh Godika Assistant Professor. University of Rajasthan. Hisar. Jaipur. Centre for Converging Technologies Professor of Botany. Jaipur E-mail: slkothari@lycos. Agriculture Research Station. Jaipur  National symposium on Cancer: Diagnosis. University of Rajasthan. S. of Zoology Uni. University of Rajasthan. English Rohit Jain .  Organized a 3 days National workshop on Bioinformatics & Biotechnology at Centre for Converging Technologies. L. Awareness & Treatment at Dept. 1984 Rakesh Kumar Jain Indian Male Single Hindi.com Dr. Director. Kothari Dean. Jaipur. Jaipur.  National seminar on Biotechnology in Sustainable Agriculture and Environmental Management at Dept. Faculty of Science. Personal Details Date of Birth Father’s Name Nationality Sex Marital Status Language Skills References: Prof. of Raj.com : : : : : : 9th August.Computer Proficiency Expert in Computers in Windows/Linux environment. MS-Excel and Adobe Photoshop. on line and off line data retrieval. Naugaon SK Agricultural University. well versed with using bio-informatics tools and software Symposium & Seminar Attended /Organized  National seminar on Water Auditing at University of Rajasthan.  Indo-US international workshop on “Plant genomics in crop improvement with special reference to biotic and abiotic stress” at CCS Haryana Agriculture University. Bikaner E-mail: shaileshgodika@gmail. of Botany.

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Details of ICMR Institutes and advanced centers can be obtained from Website: icmr. :'dja:ilhan<J03503 Subject: ICMR JRF Examination held on 15thJuly 2007. .8000/. You would be permitted to enroll yourself for pursuing Ph.com NO. Near Telephone Exchange New Colony. New Delhi . On finding placement in above-mentioned institutions you will be paid this amount from the project fund for the duration of the project. Lalsot Dai. India Phone: (Off. Kindly acknowledge the receipt of the letter. Fax: 26588662 E.D. Dear Sir/Madam.nic. Please note your stipend for JRF would be paid from project grant if you secure placement in the project. you have qualified ICMR JRF Examination held on 15th July 2007. This letter will enable you to find such placement in ICMR funded projects in these Institutions.3/1/3/Next-1 00/JRF/07 -MPD Dated: 31st August. Rakesh Kumar Jain. (Please see ANNEXURE-I). Gr. I am pleased to inform you that. K. you are found ineligible at any stage during your research work that may be due to false certification or any other reason (including computer error).110029.) 26266317 Gram: Scientific.isa. The offer is valid for the period of 2 years. 2007 11328 Rohit Jain S/o SIl. (Res. In case. as Junior research Fellow in ICMR funded Research Projects/Scheme.Dr.Mail: sinQhkeshari~vahoo.in The emoluments/stipend for JRF at present is RS. SINGH DDG (Sr. This placement is subject to fulfilling the conditions for appointment under the project/scheme.) & Chief. from any university while working in the scheme (if allowed by the university). You are advised to submit certificate of passing qualifying examination (postgraduate) and other documents by 3~thNovember 2007 to the undersigned. the award may be withdrawn by the Institution/ICMR. You are required to contact along with your Sio-data with potential Research Investigators located in various Medical colleges/Research Organization/National laboratories including ICMR Institutes who are in receipt of ICMR funding for their project for seeking placement as JRF depending upon your area of specialization.) 26589753. The Council would not be able to provide you stipend directly. K. Sainara Shawan.plus HRA as appiicabie per month. Division of Manpower Development INDIAN COUNCIL OF MEDICAL RESEARCH Ansari Nagar.

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org. 2011 onwards.… 1/1 . Centre Code Exam. IC AR …icar. 1. cut-off date) are requested to submit the same to the undersigned immediately otherwise NET certificate will not be issued and candidature for the examination will be cancelled as per the rules of the Notification. 2010 Qualified for NET Roll Number Name Father’s Name Category Physically Challenged Discipline Name Exam. No scrutiny of eligibility of the candidates has been done at this stage. IC AR C ontent updation by Mitali Ghosh Roy. 5. New Delhi-110 114. The decision of the Board in all matters of dispute shall be final. 3. Home | Privacy Policy | Disclaimer | Linking Policy | Contact us | Feedback Copyright © 2009 Indian Council of Agricultural Research Krishi Bhavan.12-04-2011 Provisional Result of National Eligibilit… Agricultural Scientists Recruitment Board Provisional Result of National Eligibility Test (NET) 2010 held on 19th September. The Board takes up the eligibility conditions with reference to documents/testimonials/certificates etc. 2. DIPA. Centre Name : 030304100200 : ROHIT JAIN : RAKESH KUMAR JAIN : UR : No : BASIC PLANT SCIENCES : 03 : BIKANER The candidate whose Roll Number is given above has qualified the NET subject to fulfillment of eligibility conditions laid down in the notification for NET-2010. no request for issue of NET certificate before January. The Candidates who had not submitted the attested copy of their Master’s Degree Certificate or Provisional Degree Certificate (completed on or before 18th September. ARIC . No further correspondence will be made in this regard.in/…/result-net-arsnet10q. ARIC . INDIA Developed and Maintained by Vivek Dubey. 2011 will be entertained under any circumstances. The qualified candidates must intimate this office about any change in their correspondence address. only after the declaration of provisional result. NET Certificate to the qualified and eligible candidate will be issued from 1st January. DIPA. 4. Therefore. Rajendra Prasad Road. The Board shall not be responsible for any technical error or typographical error of any kind for this examination.e. Dr. Note: Agricultural Scientists Recruitment Board is not responsible for any inadvertent error that may have crept in the details being published on int These Details are for immediate information to the candidates only. 2010 i.

5 mg l-1) + CC (2 mg l-1). India). coagulans. Key words: Withania coagulans. 5 and 10 mg l-1) either alone or in combination with IBA and PAA at 10. 1.ernet. antimicrobial and immunomodulatory properties (1). Plant Biochemistry & Biotechnology Vol. Addition of PG (0. Finally. July 2009 Short Communication Micropropagation of Withania coagulans (Stocks) Dunal: A Critically Endangered Medicinal Herb Rohit Jain1. coagulans are also used for milk coagulation (2). MS (7) basal medium supplemented with 3% sucrose. USA) were also tested with optimal cytokinin concentration. Philips. PG phloroglucinol. PAA . IAA . 249-252.α-naphthaleneacetic acid. pH adjusted to 5. 1. Jaipur 302 004. RAPD . and cultured on MS medium enriched with BA (0. coagulans occurs through seeds but chances of seed setting get limited due to unisexual nature of flowers.6-benzylaminopurine. 2. Jaipur 302 004. The natural propagation of W. The in vitro-raised microshoots (2–3 cm in length) were harvested for rooting. IBA . Kn (0. Prolific multiplication of axillary buds occurred from the nodal segments taken from adult plant. E-mail: kothari-sl@uniraj.25 mg l-1) + PAA (0. Two step rooting procedure was followed. Fruits of W.8 before *Corresponding author. Various concentrations (0. In step two. This protocol has the capacity of producing 1000 plants from one nodal segment after 4 subcultures of 2 weeks each. Coagulin-H (1). choline chloride. 2 and S L Kothari1. there have not been any reports of ex situ conservation of this plant through tissue culture. phloroglucinol. CC . The extract of the plant exhibits free radical scavenging (3) and hypolipidemic activity (4). the explants were thoroughly washed with sterile distilled water and inoculated onto MS medium supplemented with BA or Kn at 0.5 mg l-1) and PG (0.5. Only two plants of Withania coagulans were spotted in the wild in Ajmer district and the explants were taken from one of these plants.5. To date. India An efficient micropropagation protocol has been developed for Withania coagulans.indole-3-acetic acid. 50 and 100 mg l-1 for 7 days on MS liquid medium using a filter paper bridge. agar-gelled semisolid medium . coagulans (5) has been identified as immunosuppressive drug (6). University of Rajasthan. followed by an aqueous solution of 0. 1. 2. 2. Step one involved the pulse treatment of individual shoots with PG or CC (0.kinetin. Withania species (Solanaceae) are the natural source of withanolides (steroidal lactones) which have potential antitumor. surface sterilized with 70% (v/v) ethanol for 30s. 18(2). Shoot buds induced in primary cultures were sectored in clumps of 3-4 and cultured on fresh medium for further multiplication of shoot buds. Arunima Sinha1.choline chloride. India Centre for Converging Technologies (CCT). NAA . Sumita Kachhwaha1. Nodal segments from field grown plant were thoroughly washed in 5% (v/v) Teepol. USA) and CC (Sigma. University of Rajasthan.random amplified polymorphic DNA autoclaving at 1.in Abbreviations: BA .phenylacetic acid. We now report an efficient and reproducible protocol for micropropagation of W. micropropagation.5 mg l-1) for the initial 7 days’ pulse treatment and thereafter. the pre treated microshoots were transferred onto ½ or ¼ MS.indole-3-butyric acid.5 mg l-1). Nodal segments and shoot tips of elongated microshoots also behaved the same way in cultures and formed multiple shoots through axillary bud multiplication. 5 and 10 mg l-1) of PG (Sigma. 2* 1 2 Department of Botany. they were transferred to rooting medium containing IBA (0. The cultures were incubated at 25 ± 1 ° C under a 16-h photoperiod with 25µmol m -2 s -1 photosynthetic photon flux density (PPFD) provided by cool white fluorescent tubes (40 W.J. 3 and 5 mg l-1 either alone or in combination. isolated from W.06 kg cm-2 (121°C) for 20 min was used in all the experiments.1% (w/v) freshly prepared HgCl2 solution for 3 min. a highly endangered medicinal herb and an important natural source of withanolides.5.5 mg l-1). Overexploitation and the reproductive failure have rendered the species highly vulnerable to complete extinction. Elongated shoots were placed on filter paper bridges soaked in MS medium with CC (10 mg l-1) and PG (0. Kn .5 mg l-1) in the regeneration medium significantly improved induction and elongation of shoot buds.

2e ± 0.2 0.1 0.3 0.4 0.1 0. Each explant formed up to 21 shoot buds but these were too short (0.5h ± 19.5 mg l-1) along with BA (0. coagulans (excised from in vitro raised shoots) cultured on MS medium supplemented with BA (0.4d ± 19.5f 21. Fig.4b 21. 0.5 1 2 3 5 0 0 0 0 0 0.5c ± 3.2g ± 0. Shoot bud formation from nodal segments of W.7g 14.5 1 2 3 5 0.4 6. The data was analyzed statistically using one –way analysis of variance (ANOVA) by Fischer’s least significant difference (P = 0.1 0.3 0.4 9.E.4f ± 0. E.1 0.5a ± 4.3-0.3 0. The data on shoot formation and rooting were collected after 4 weeks. 50 ° C for 45 seconds and 72°C for 1 min.05 from each other .5i 13.1 0.5 Kn (mg l-1) 0 0 0 0 0 0. The explants inoculated on MS medium responded differently on BA and Kn (Table 1). All experiments were repeated twice.3 0. and a final extension at 72°C for 5 min. Each treatment consisted of twenty replicates.1 0.5f ± 23.0d ± 0.3e ± 17.1d ± 3.0x ± 0.6 0.E.1 0.5 2. Plantlets were then removed from the vessels.7d ± 0. 0.2 0.5 mg l-1) proved best for induction of multiple shoots.8a ± 0.6d ± 4.5 mg l-1) in combination with Kn (0.2c ± 3.E.5 0.9d ± 3.3h 13. An average of 19 shoots (1cm) could be Table 1.1 0.3b ± 4.5 0.0c 17.2 5.2 0. Three explants were cultured per conical flask and single explant was cultured per test tube.4 11.4bc ± 4. Nodal segments and shoot tips were also used from regenerated shoots after 4 weeks of shoot bud initiation.2 0.5 0.5j ± 0. – Standard error Means in a column followed by different letters are significantly different at P = 0. Twelve RAPD primers were taken to assess the clonal fidelity of the regenerated shoots.5 1 2 3 5 0 0 0 0 0 -1 CC (mg l ) 0 0 0 0 0 0 0. BA (0.2 0.5 S. Histological preparations were made as described (8).2 0.4g ± 2. an initial denaturation at 94°C for 5 min followed by 35 cycles of 94°C for 30 sec.9a 23.1 3.25 – 1 mg l-1) either alone or in combination.2 0.5 cm). The sample was powdered in liquid nitrogen (196°C) and stored at -20°C until use for DNA extraction by CTAB method (9).3e 15. 3. 10).3 Mean length of Shoots (cm) ± S.4 0.0c ± 0.5 mg l-1) in MS medium improved the establishment of nodal explant cultures (Table 2.7d ± 3.2 0. The PCR amplification conditions were.E.2d 18. 20. PG is a phenolic compound that stimulates shoot and root growth in shoot cultures (11).E.2 0.4a ± 21.2 0. 1b).8e ± 3.4 3.2g ± 0.2 0.5 mg l -1) and different concentrations of PG or CC PG (mg l ) 0 0.5 mg l -1) + Kn (0.3 0.1 0. Shoot bud formation from nodal segments and shoot-tips of W.0d ± 3.3f ± 2.5 mg l-1) and Kn (0. and not suitable for micropropagation.3 0.3 0. E.1j ± ± ± ± ± ± ± ± ± ± ± 0.4d ± 2. BA gave better response than Kn in terms of induction of shoot buds.1 0.3 3.6b ± 23.1i ± 14.3 Mean length of Shoots (cm) ± S.4 7.3c ± 20. The use of PG during Table 2. coagulans cultured on MS medium supplemented with BA and Kn BAP (mg l-1) 0. The addition of PG (0.0c ± 0. washed gently with water and transferred to pots containing 1:1 mixture of garden soil and organic manure. Cultures were evaluated after 4 weeks.1b ± 3.5 mg l-1) + Kn (0. of Buds/Explant ± S. 1a).1 Shoot-tips Mean No.3 0.4b ± 0.1 S. Fig.5 1 2 3 5 -1 Nodal segments Mean No.3 18.3a ± 24.3 0.250 J Plant Biochem Biotech with 3% sucrose supplemented with IBA /IAA/ NAA/ PAA (0.7b ± 4. Proliferating shoot cultures were established by subculturing the shoots on MS medium with BAP (0.0 0.05. of Buds/Explant ± S. – Standard error Means in a column followed by different letters are significantly different from each other obtained after 3 weeks (Table 1. 22.5 Percent response (%) 57 68 74 79 83 43 55 55 66 73 83 Mean No. DNA was extracted from the leaves of 19 randomly selected regenerated plants and from the leaves of mother plant (WM).3e ± 3.6be ± 4.6 h ± 0.9e ± 0. of Buds/Explant ± S.5 mg l-1) in clumps of 3-4 buds.1c 19.9g ± 22.2 0.1 0.2 0.3a ± 4.8d ± 2.

and (f) Agarose gel electrophoresis of RAPD fragments of W.5 mg l-1) + PG (0.25 mg l-1) + PAA (0.5 mg l-1) + Kn (0. (e) Rooting on half strength MS medium with IBA (0.5 mg l-1) + CC (2 mg l-1). (c-d) Histological details of the shoot bud formation from the shoot tip (c) and nodal segments (d). In vitro regeneration of W. coagulans showing banding patterns of 20 plants amplified by the primer OPA-19.Short Communication 251 Fig.5 mg l-1) + Kn (0. (a) Induction of shoot buds from nodal explants of W. 1.5 mg l-1). . coagulans cultured on MS medium with BA (0.5 mg l-1). coagulans. (b) Proliferation and elongation of shoot buds on MS medium with BA (0.

1c). 36 (2000) 319. Ibanez MR & Amo-Marc JB. Mangalore (2004) p 294. Voelter W. Hemalatha S. Plant microtechnique. 52 (2008) 743. A ring of multiple shoot primordia could be observed arising directly from base of cultured shoot tip (Fig. 6 7 8 9 10 11 12 13 14 15 16 17 . 43 (2006)1855. K Rajendra. 8 generated distinct.7) of roots and root length (7. New Delhi for the financial support in the form of a R&D project: CSIR-38(1178)/EMR-II/07. 93 (2004) 261. Rastogi et al (13) have also advocated incorporation of PG in the medium for better growth of cultures. Biol Plant. About 75% of the micropropagated plants survived after transfer to soil and organic manure (1:1).3cm) were seen after pulse treatment of shoots in MS medium containing 10 mg l-1 CC and 0. Jodhpur. S Nivas. Plant Sci. USA (1940). 67 (1999) 27. CC and PG have enhanced rooting in Bambusa tulda (15). Statistical procedures for agricultural research. Gul W.5 mg l-1) and Kn (0. 153 (2004)73. Heterocycles. 2009.25 mg l-1). Patel PK. A total of 580 amplification products were detected. The incorporation of CC at different concentrations enhanced the response of rooting of shoots significantly. Mol Immun. Maurya R. Abdullah NR. Focus. India (1995) pp 246. followed by their establishment in pots containing (1:1) soil and manure in greenhouse. Diwanay S. In the cultured nodes. In Vitro Cell Dev Biol-Plant. The primers OPA-5 and OPA-19 (Fig. The protocol offers a potential system for a large-scale propagation and conservation of this medicinal plant and would facilitate its improvement programme using genetic transformation and metabolic engineering techniques. Shirin F & Ansari SA. Flora of the Indian desert .252 J Plant Biochem Biotech multiplication has improved shoot multiplication in several species (12). Of 12 random primers. Clonal fidelity of the regenerated shoots was checked through RAPD. Plant Cell Tiss Org Cult. 60 (2000) 139. Faivre-Rampant O. John Wiley and Sons. 18 (2008) 6534. 26 (1998) 49. All the established plants were apparently uniform and did not show any detectable variation. Murad S.5±0.9±0. Bhandari MM. Rohit Jain and Arunima Sinha also thank CSIR for the award of Senior Research Fellowships. Similar results have been obtained in present investigation. Bioorg Med Chemis Lett. J Ethnopharmacol. Rastogi S. 2009. Johansen DA. Jens F & Naz A. Patki P & Patwardhan B. Gomez KA & Gomez AA. 15 (1962) 473. SAC. New York. Ahmad A & Choudhary MI. 2009. In Biotechnology for a better future (L D’Souza. Inc. Choudhary MI. Received 20 January. Qureshi S. 115 (2008) 315. New York (1984) Sarkar D & Naik PS. PAA (0. Husain MK & Anis M. Plant Growth Reg. These compounds are reported to enhance rooting by acting as auxin protectors to increase the free endogenous IAA levels during the inductive phase of rooting (16). MPS Repros. Rizvi SMH. Yadev S. The plantlets were successfully hardened inside the culture room under diffused light on MS medium for 2 weeks.5 mg l-1). at 2-wk interval. Online published 18 July. Ismail Z. Singh AB & Srivastava AK. Singh RP & Dwivedi UN. Hoff A. on MS medium supplemented with BA (0.5 mg l-1) and CC (2 mg l-1) after 7 days (Fig. 1f) gave highly reproducible banding pattern. Mishra Y. 48 (1998) 1801. Singh PN &Chansouria JPN. 12 (1990) 13. J Ethnopharmacol. Mesaik MA. Atta-ur-Rahman.5 mg l-1 PG followed by their transfer to ½ strength MS medium with IBA (0. Doyle IJ & Doyle JL. Zaheer-ul-Haq. Jayendra A. Rani V & Raina SN. Yousaf M. 1e). Fingerprinting profiles of regenerants were monomorphic and there was no variation amongst mother and tissue culture raised plants. M Anuradha. The maximum frequency of root formation (80%). a distinct meristematic zone of small densely stained cells was present over a differentiated zone. vertical and sideways expansion of the meristematic zone occurred (Fig. Physiol Plant. Murashige T & Skoog T. Wahi AK. Sci Hort. Acknowledgements We thank Council of Scientific and Industrial Research (CSIR). Kevers C & Gaspar T. Editors). accepted 8 July. S Hegde. There are number of reports demonstrating the suitability of enhanced axillary branching for raising true to type plants (17). 1d). highest number (11. at a later stage of development. reproducible products. Atta-ur-Rahman. Mc Graw-Hill Book Company. Gill HK. Two-step procedure for rooting has been used to advantage in several woody species (14). The ability of shoot multiplication was maintained up to 12 subcultures. Histological studies revealed that in the axil of each leaf. Yousaf M Siddiqui RA.. References 1 2 3 4 5 Agarwal R.

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Tissue and Organ Culture (PCTOC) Journal of Plant Biotechnology ISSN 0167-6857 Volume 105 Number 1 Plant Cell Tiss Organ Cult (2011) 105:135-140 DOI 10.1007/s11240-010-9840-3 1 23 .Adventitious shoot regeneration and in vitro biosynthesis of steroidal lactones in Withania coagulans (Stocks) Dunal Plant Cell.

provided it is not made publicly available until 12 months after publication. please use the accepted author’s version for posting to your own website or your institution’s repository. This e-offprint is for personal use only and shall not be selfarchived in electronic repositories..V. 1 23 . If you wish to self-archive your work.Your article is protected by copyright and all rights are held exclusively by Springer Science+Business Media B. You may further deposit the accepted author’s version on a funder’s repository at a funder’s request.

Sinha Á D. Well-rooted plants were transferred to a greenhouse for hardening and further growth. Kothari • Received: 5 June 2010 / Accepted: 30 August 2010 / Published online: 19 September 2010 Ó Springer Science+Business Media B. Shoot bud proliferation occurred through both adventitious and de novo routes depending on the hormonal regime of the culture medium. antihyperglycemic. Keywords Micropropagation Á HPLC Á TLC Á RAPD Á Withania coagulans Á Withanolides Abbreviations BA 6–benzyladenine CC Choline chloride DAD Diode array detector IAA Indole–3–acetic acid IBA Indole–3–butyric acid Kn Kinetin MS Murashige and Skoog NAA a–naphthaleneacetic acid PAA Phenylacetic acid PG Phloroglucinol RAPD Random amplification of polymorphic DNA TLC Thin layer chromatography Introduction Withania coagulans (fam.V. L. anti-inflammatory. 1. cardiovascular. antitumor.3 lM) while multiple adventitious shoot bud differentiation occurred on medium fortified with 2. L.3 lM CC for 3 weeks. Solanaceae) is commercially important for its ability to coagulate milk.Plant Cell Tiss Organ Cult (2011) 105:135–140 DOI 10.6 lM choline chloride (CC) and 3. Kothari (&) Centre for Converging Technologies (CCT). 2010 Abstract A micropropagation system through leaf explant culture has been developed for Withania coagulans.2 lM IBA. Jaipur 302004. consumption and sensile debility (Bhandari 1995). University of Rajasthan. rheumatism.2 lM BA. immunosuppressive.com increased up to twofold and that of withanone up to tenfold. Shoot buds were transferred to proliferation medium containing 2. Jain Á S. Jain Á A. 2. India S.3 lM Kn.2 lM). Kachhwaha Á S. free radical scavenging and central nervous system depressant activities of the 123 . Direct regeneration via leaf explants will be useful for Agrobacterium-mediated genetic transformation.3 lM kinetin (Kn) and lower levels of 6–benzyladenine (BA) (13. Jaipur 302004. Green compact nodular organogenic callus developed on Murashige and Skoog (MS) medium supplemented with 2. in the treatment of ulcers. Kothari Department of Botany. Random amplification of polymorphic DNA (RAPD) showed monomorphic bands in all the plants thereby confirming clonality of the regenerants. and 3. 3. L. Accumulation of withaferin A and withanolide A R.9 lM phloroglucinol (PG) for further growth and development of shoot system. Thin layer chromatography (TLC) showed the presence of withanolides in the regenerated plants.6 lM PAA.3 lM kinetin (Kn) and higher levels of BA (22.1007/s11240-010-9840-3 RESEARCH NOTE Adventitious shoot regeneration and in vitro biosynthesis of steroidal lactones in Withania coagulans (Stocks) Dunal Rohit Jain • Arunima Sinha • Devendra Jain Sumita Kachhwaha • S. India e-mail: slkothari@lycos. and will facilitate pathway manipulation using metabolic engineering for bioactive withanolides. Kachhwaha Á S.9 lM PG and then transferred to rooting medium containing ‘ MS. hepatoprotective. Quantification through reverse-phase HPLC revealed increased concentration of withanolides in the regenerated plants compared to the field-grown mother plant. Antimicrobial. University of Rajasthan. and 14. dropsy. Elongated shoots were rooted using a two-step procedure involving pulse treatment of 7 days in a medium containing 71.

1% acetic acid. All experiments were repeated twice. Various concentrations and combinations of different plant growth regulators (Sigma. Dept. The in vitro shoot cultures could provide an alternative to field plant harvesting for the production of therapeutically valuable compounds (Sangwan et al. Jaipur. 71. 2007). India) solution for 3 min. Philips. 2. Agilent) using water (A) and methanol (B).8 lM). Explants were thoroughly washed under running tap water for 15 min followed by treatment with 20% Extran (liquid detergent.2 lM IBA.2 and 3. Withaferin A. The leaf samples were powdered in liquid nitrogen and stored at -20°C until used for DNA extraction by CTAB method (Doyle and Doyle 1990). each containing 0. 80 ml water and 10 ml 60% perchloric acid) followed by heating at 110°C. India) gel electrophoresis and photographed using Gel Documentation System (Bio-Rad.6 lM) and a–naphthaleneacetic acid (NAA. The species was identified by the Herbarium. Cultures were maintained at 26 ± 1°C under 16/ 8 h photoperiod with 25 lmol m-2 s-1 photosynthetic photon flux density provided by white fluorescent tubes (40 W. coagulans towards the verge of extinction (Jain et al.0. The pH of the medium was adjusted to 5. of Botany.3 lM Kn) containing 3.9 lM PG and then transferred to rooting medium containing ‘ MS. Three explants per flask and single explant per test tube was cultured. Merck. India) for 5 min.8.3 lM CC prior to hardening as described previously (Jain et al. Qualitative and quantitative analysis of withanolides Qualitative withanolide profiling was done through TLC while quantification was carried out through HPLC as described by Sangwan et al. particle size 1. 13. and 14. 4. 37°C for 45 s and 72°C for 2 min.6 and 2.5 mm 9 150 mm. (2007).6. The numbers of responding explants 123 . and a final extension at 72°C for 10 min.136 Plant Cell Tiss Organ Cult (2011) 105:135–140 plant have also been demonstrated (Maurya and Akanksha 2010). phenylacetic acid (PAA. the explants were aseptically surface sterilized with 0.2 lM BA. 2009b).4. 8. 4. 1998). as solvent and online UV-Diode Array Materials and methods Plant material and establishment of in vitro cultures from leaf explants Leaf explants (0.1. somnifera and W. Explants were rinsed 4–5 times with sterile distilled water and cultured on full. Regenerated shoots of appropriate length ([3 cm) were subjected to a two-step rooting procedure involving pulse treatment of 7 days on ‘ MS.2 and 22.3. withanolide A and withanone are the major withanolides present in W.6 lM PAA.9. India) and 0. Leaf explants with or without petiolar parts were placed abaxially on the medium.2. Isolation of withanolides from various tissues was performed using the method described by Sangwan et al.2% agarose (Himedia. India) including 6–benzyladenine (BA. For TLC.6 lM) were added in the medium to optimize growth and differentiation. 1.6 lM choline chloride (CC) and 3.2 lM). 3. v/v). 13. and shoot buds developed per explant were recorded and shoot buds were subcultured on first stage proliferation medium (MS. University of Rajasthan.2. The data on shoot bud formation and rooting were collected after 4 weeks. we report regeneration from leaf explants and production of withanolides from the regenerated plants for the first time.8 lm. indole-3butyric acid (IBA. 1.and half-strength MS (Murashige and Skoog 1962) medium supplemented with 3% sucrose (Merck. Extraction of withanolides All the analytical and HPLC grade solvents. Pharmacological investigations have elucidated association of these activities with the specific steroidal lactones known as withanolides present in Withania (Attaur-Rahman et al. reagents and precoated silica gel TLC plates were purchased from Merck. RAPD analysis DNA was extracted from the leaves of 17 randomly selected regenerated plants and from the leaves of mother plant (WM).9% agar (bacteriological grade. Merck. 2.4 lM). performed in a solvent system consisting of chloroform:ethyl acetate:methanol:toluene (74:4:8:30. India).9 lM phloroglucinol (PG) to further enhance growth and development of shoot buds. The amplicons were separated through 1. and 2. (2007). indole-3-acetic acid (IAA. and development was done with anisaldehyde reagent (250 ll anisaldehyde in a mixture of 20 ml acetone. There are no reports of in vitro plant regeneration in W. The PCR amplification conditions were: an initial denaturation at 94°C for 4 min followed by 40 cycles of 94°C for 45 s. Germany).8–2 cm) were collected from the fieldgrown plants spotted in Ajmer (Rajasthan) in 2007. Eventually. 1. 1.4 and 2.8 followed by sterilization at 1. coagulans. 2009b). 2. 4.1% (w/v) HgCl2 (Merck. HPLC analysis was performed on Agilent (Germany) model 1200 and separation was achieved by a reverse-phase column (Eclipse XDB c-18. 1. 2009b). kinetin (Kn. Overexploitation and the reproductive failures forced the species W.7 and 2. Here.4. 1.2 kg/cm2 pressure and 121°C temperature for 20 min. 10 ll sample was loaded on precoated silica gel G-60 plates.2 lM). 0. 9. India). 2. 1. coagulans except our earlier report using nodal and shoot tip explant cultures (Jain et al. Twenty replicates were maintained for each treatment.9 and 23.

05) (Gomez and Gomez 1984). Authentic withanolides including withaferin A. Kn alone (Murch et al. Sharma et al.5) regenerated mostly from petiolar base of leaf explants or at leaf midrib region on medium supplemented with 22.1 ± 0. 2007).2–13. Sinha et al. Kothari et al. Kn in combination with auxins initiated formation of pale and non–morphogenic callus.5 a Detector (UV-DAD) at 227 nm.6 ml min-1. A total of 1. Twenty random primers (OPF 1–10 and OPT 1–10) were used. A combination of 2. Use of CC and PG in enhancing rooting has also been reported in Dendrocalamus hamiltonii (Sood et al.6 lM PAA. The amount of callus increased with increasing concentration of auxins. 2010. Therefore.7 ± 0. Elongated shoots ([3 cm) were transferred to ‘ MS medium containing 1. of which 15 produced distinct and reproducible bands. but it could not induce any shoot buds. The use of 2. 1e) were successfully transferred to the greenhouse for hardening.1 lM) or IBA (0. serotina (Liu and Pijut 2008). 2003.2 lM BA and 2.6 ± 0. 2010). Prunus persica (Gentile et al.197 amplicons were obtained and primer OPF-3 generated a highly 123 . we also examined the effect of IAA.05 Kn (lM) 2. Combination of BA with Kn for inducing shoot bud differentiation from the explants has also been reported in several other plants (Dayal et al. but the elongation of the shoot buds did not occur (Fig. whereas they responded with enlargement and swelling at the cut petiolar end followed by callus formation on MS medium supplemented with Kn (2. 2002) and Bambusa tulda (Mishra et al. a length which was required for rooting (Fig. Rhizogenesis was observed all along the lamina cultured on medium with BA (2.3 lM Kn and 3.3 ± 0. NAA or PAA in combination with BA or Kn on organogenesis. Results and discussion Leaf explants cultured in the absence of growth regulators senesced without producing callus or adventitious buds. 3. 2009) or in combination with auxins (Koroch et al. 10:90. 1b). withanone and withanolide A (Chromadex. The regenerated plants were subjected to RAPD analysis to check their clonality. and 14. 1c). 2008).2 lM IBA.9 lM PG for rooting.Plant Cell Tiss Organ Cult (2011) 105:135–140 137 Table 1 Shoot bud formation from leaf explants of W. The combination of BA or Kn with auxins was not conducive to organogenesis. Previous reports have shown the same impact including petioles for enhancing shoot regeneration in several other plant species such as Paulownia tomentosa (Corredoira et al. 1a). USA) were used as markers to ascertain their discrete resolution from each other under these conditions for both TLC and HPLC. This clearly demonstrated that the combination of BA and Kn was the most important factor for shoot regeneration from leaf explants of W.4 lM). 2008).6 ± 0.2–22. 45–60 min at a flow rate of 0. nodular callus was observed on medium supplemented with BA (13.2 SE Standard error Means in a column followed by different letters are significantly different from each other at P = 0. Brown.3 lM CC after 7 days of pulse treatment with 71. 2004) and BA alone (Kulkarni et al. 2009a.3 lM).0– 2. Tilkat et al. 0–45 min. Sample volume of 10 ll was injected and the column temperature maintained at 27°C during the run.6 lM CC and 3.2 lM BA and 2.3 lM Kn (Table 1.2 lM) and IAA (1. coagulans cultured on MS medium supplemented with BA and Kn BA (lM) 2. Sreedhar et al. HPLC data was analyzed with the Chemstation LC– 3D software (Agilent). Computation of withanolide concentration in the samples was done through a calibration curve of concentration versus detector response (peak area) using different concentrations of standard solutions of withaferin A.5 e 7.3 2. 2003. 60:40–25:75. PG has similarly been used by other workers (Sarkar and Naik 2000. The rooted plantlets (Fig. 2004) or in combination with auxins (Kachhwaha and Kothari 1996. 2008).9 lM PG was required in the proliferation medium for the elongation of shoot buds up to 2–3 cm.9 lM) or PAA (1. Presence of petiolar part along with lamina was essential for morphogenesis as no response was observed when lamina without petiolar part was cultured. 2002. Zhou et al. 2010) have most frequently been reported to induce in vitro plant regeneration in a wide range of monocotyledonous and dicotyledonous plants. Feeney et al.2 lM) with NAA (1. These compounds have been reported to act as auxin protectors and increase the endogenous IAA levels during the inductive phase of rooting (Faivre-Rampant et al.3 lM) promoted the initiation and development of shoot buds along with callus (Fig.3 lM) or BA (2.3 % response 80 86 73 93 80 Shoot buds (Mean ± SE) 4. Shoot buds induced on explants in the primary cultures were transferred to the proliferation medium containing 2.3 2. The incorporation of CC and PG enhanced rooting significantly. 2002.2 b 17. Clusters of adventitious shoots (17.3 2. coagulans. Jain et al. 2000.2 4.3 lM Kn for further differentiation of new shoot buds. withanolide A and withanone in methanol.2 lM BA.6 ± 0. 2004).2–13. compact. 1d).6 c 12. 2. and P.3 lM Kn in combination with BA (2.4 8. Reddy et al.3 2.6 d 9. The data was analyzed statistically using one-way analysis of variance (ANOVA) by Fischer’s least significant difference (P = 0.6 lM).9 13.3 22. Baskaran and Jayabalan 2005.3–22. The solvent gradient was set as A:B. CA. Fig.

and standard samples of withaferin A. 7 samples extracted from field roots the amount of respective withanolide over a range of 50–1. TLC of different extracts revealed that withaferin A. b Direct induction from petiolar end on MS. Sangwan et al. 2007). 13. The study used an analytical reverse phase HPLC system providing symmetrical and high resolution peaks of three important withanolides in the plant. 2 standard withanolide A. 3). 4 sample extracted from in vitro shoots.46 min. 2004. 2 Agarose gel electrophoresis of RAPD fragments showing banding pattern amplified by OPF–3 primer. The identification of withanolides was confirmed on the basis of retention time and absorption spectra on UV-DAD (32. Analysis of withanolide content in in vitro shoot cultures of W. 6 samples extracted from callus. Withanolide content was analyzed by HPLC. somnifera has been reported by several workers (Ray and Jha 2001. a Indirect induction on MS. 5 samples extracted from field leaves.2 lM IBA.3 lM BA and 2. 3 TLC profile of W. 3 standard withanone. 3. 1 Shoot bud induction from leaf explants of W.2 lM BA. 2.6 lM PAA and 14.3 lM Kn. coagulans. 215 nm.3 lM CC reproducible banding pattern (Fig. Lanes 1 standard withaferin A. withanolide A and withanone were used to construct a calibrated graph by plotting peak areas versus Fig.000 ng ll-1.9 lM PG. withanolide A and withanone were biosynthesized in regenerated plants of W. c Shoot buds developed on the first stage proliferation medium. 22. The response was linear over the tested concentration range. but there are no such reports for W. DNA fingerprinting profiles of regenerants revealed that there was no variation amongst mother and tissue culture-raised plants.38 min.3 lM Kn. coagulans. coagulans (Fig. C control Fig.2 lM BA and 2.3 lM Kn and 3.138 Plant Cell Tiss Organ Cult (2011) 105:135–140 Fig. d Proliferation and elongation of shoots on MS. There are many reports demonstrating the suitability of enhanced axillary branching for raising true-to-type plants (Rani and Raina 2000). M Molecular marker. 38. 1. 123 . 2). e Rooting on ‘ MS. coagulans. 2.

4e) and more in roots (Fig. The results also 123 . Taken as a whole. A shift towards organ differentiation resulted in improved potential of the cultures to synthesize withanolides.009 0.014 0.Plant Cell Tiss Organ Cult (2011) 105:135–140 139 Fig. and 40.90 min. 4d.009 Withanone 0. 4b) and withanone (Fig. e field leaves. Samples from d in vitro developed shoots. and f field roots (insets are UV-DAD spectra of the specified withanolide) 230 nm. The detection of higher content in differentiated cultures also points out that the enzymes responsible for biogenesis of withanolides in vitro might be optimally active in the culture conditions as has been shown earlier in W. the response is highly sensitive and directly related to the combinations of exogenous growth regulators in the culture medium. Several factors. withanolide A (Fig. the difference in chemotype utilized as source for initiation of multiple shoot buds.031 ± 0. 2007).084 ± 0.001 0. Withanolide A accumulates in small amounts in shoots (Fig. e). b withanolide A. The accumulation of all the three withanolides was higher in regenerated plants than in the samples taken from field-grown plants (Fig. and culture conditions such as basal media composition and growth regulator types utilized to establish cultures might have contributed to withanolide production. coagulans have a great organogenic potential for shoot bud formation.059 ± 0. but in the present study the amount of withanolide A was as good in regenerated shoots as in the roots of field plants (Table 2. 4a). 4c). 4f) in field-grown plants. a Withaferin A.004 0.113 ± 0. The quantities of withaferin A and withanolide A increased up to two-fold while the withanone content increased up to ten-fold in the regenerated plantlets as compared to field-grown plants (Table 2). 230 nm for withaferin A (Fig. Fig.006 Nil synthesis is regulated in a tissue-specific way and organogenesis is the key regulatory factor which stimulates production of withanolides in vitro. c withanone..282 ± 0. however. coagulans Sample Withanolide Content (mg gfw-1) Mean ± SE Withaferin A Field leaves In vitro leaves Field roots 0. somnifera (Sharada et al.005 Nil Withanolide A 0. 4d). respectively).123 ± 0.192 ± 0. our results demonstrate that leaves of W. e. The positive correlation between withanolide synthesis and morphological differentiation suggests that Table 2 Withanolide content in different tissues of W. 4 DAD–HPLC chromatogram of standards.g.

Kevers C. Arunima Sinha and Devendra Jain thank CSIR for the award of Senior Research Fellowships. Vij SP. Gaspar T (2004) IAA–oxidase activity and auxin protectors in nonrooting. Vieitez AM (2008) Thidiazuron-induced high-frequency plant regeneration from leaf explants of Paulownia tomentosa mature trees. Choudhary MI. Suri KA. Jain R. Plant Sci 153:73–80 Feeney M. Sharma OP. Plant Cell Tissue Org Cult 90:201–214 Gentile A. J Plant Biochem Biotechnol 18:249–252 Kachhwaha S. Ahuja A. Plant Cell Rep 21:1072–1079 Doyle JJ. Rodrigues R. Sharma M. Plant Cell Tissue Org Cult 95:197–208 Dayal S. Sharma KK (2003) An efficient protocol for shoot regeneration and genetic transformation of pigeonpea [Cajanus cajan (L. Biotechnol Adv 28:35–48 123 . Ayaz E (2009) Direct plant regeneration from mature leaf explants of pistachio. Kachhwaha S. Gomez AA (1984) Statistical procedures for agricultural research. Sinha A. 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Kothari SL (2009a) Improved micropropagation protocol and enhancement in biomass and chlorophyll content in Stevia rebaudiana (Bert. Kothari SL (1996) Plant regeneration from immature embryo explants of Hordeum spontaneum and Hordeum vulgare. Bhagwat B. Planta Med 67:432–436 Reddy PS. Raina SN (2000) Genetic fidelity of organized meristem— derived micropropagated plants: a critical reappraisal. Li M.140 Plant Cell Tiss Organ Cult (2011) 105:135–140 Kulkarni AA. Biol Plant 51:161–164 Sharma PK. Kachhwaha S. a main medicinal plant in ayurveda. ex munro. p 680 Jain P. Gul W.

..1 Download▼ Save▼ Gordonia lacunae strain CCC12 16S ribosomal RNA gene. Jain.>1491 /product="16S ribosomal RNA" ORIGIN 1 gggcaaacgc tggcggcgtg cttaacacat gcaagtcgaa cggaaaggcc cagcttgctg 61 ggtactcgag tggcgaacgg gtgagtaaca cgtgggtgat ctgccctgca ctctgggata 121 agcctgggaa actgggtcta ataccggata tgaccaactg tcgcatggtg gttggtggaa 181 agcttttgcg gtgtgggatg ggcccgcggc ctatcagctt gttggtgggg taatggccta 241 ccaaggcgac gacgggtagc cgacctgaga gggtgatcgg ccacactggg actgagacac 301 ggcccagact cctacgggag gcagcagtgg ggaatattgc acaatgggcg caagcctgat 361 gcagcgacgc cgcgtgaggg atgacggcct tcgggttgta aacctctttc accagggacg 421 aagcgtgagt gacggtacct ggagaagaag caccggccaa ctacgtgcca gcagccgcgg 481 taatacgtag ggtgcgagcg ttgtccggaa ttactgggcg taaagagctc gtaggcggtt 541 tgtcgcgtcg tctgtgaaat tctgcaactc aattgcaggc gtgcaggcga tacgggcaga 601 cttgagtact acaggggaga ctggaattcc tggtgtagcg gtgaaatgcg cagatatcag 661 gaggaacacc ggtggcgaag gcgggtctct gggtagtaac tgacgctgag gagcgaaagc 721 gtgggtagcg aacaggatta gataccctgg tagtccacgc cgtaaacggt gggtactagg 781 tgtgggttcc ttttcacggg atccgtgccg tagctaacgc attaagtacc ccgcctgggg 841 agtacggccg caaggctaaa actcaaagga attgacgggg gcccgcacaa gcggcggagc 901 atgtggatta attcgatgca acgcgaagaa ccttacctgg gtttgacata caccagacgc 961 ggctagagat agtcgttccc ttgtggttgg tgtacaggtg gtgcatggct gtcgtcagct 1021 cgtgtcgtga gatgttgggt taagtcccgc aacgagcgca acccttgtcc tgtattgcca 1081 gcgggttatg ccggggactt gcaggagact gccggggtca actcggagga aggtggggat 1141 gacgtcaagt catcatgccc cttatgtcca gggcttcaca catgctacaa tggctggtac 1201 agagggctgc gataccgtga ggtggagcga atcccttaaa gccagtctca gttcggattg 1261 gggtctgcaa ctcgacccca tgaagtcgga gtcgctagta atcgcagatc agcaacgctg 1321 cggtgaatac gttcccgggc cttgtacaca ccgcccgtca cgtcatgaaa gtcggtaaca 1381 cccgaagccg gtggcctaac cccttgtggg agggagctgt cgaaggtggg atcggcgatt 1441 gggacgaagt cgtaacaagg tgccgtaccg gaagcacatt tatattttgg g // Write to the Help Desk NCBI | NLM | NIH Department of Health & Human Services www.nlm.. India » See more.1 GI:291061266 KEYWORDS .. and Kothari.V..G. Rajasthan 302004..nih.Gordonia lacunae strain … My NCBI [Sign In] [Register] All Databases PubMed Nucleotide Protein Genome Structure OMIM PMC Journals Books Search Nucleotide Limits Preview/Index for History Clipboard Details Go Clear Format: GenBank FASTA Graphics More Formats▼ GenBank: GU727686.. Gordonia lacunae strain SOURCE Gordonia lacunae CCC12 16S ribosomal RNA ORGANISM Gordonia lacunae Bacteria.. Sharma. Kachhwaha. centre for converging REFERENCE 1 (bases 1 to 1491) tec.gov/…/291061266 1/2 . Recent activity ACCESSION GU727686 Turn Off Clear VERSION GU727686. JLN Marg.ncbi. partial sequence. Gordonia.P.22-03-2010 Nucleotide ... Jaipur. GU727686 (1) Actinomycetales. Actinobacteridae.. Agarwal. University Gordonia (730) of Rajasthan. (8) AUTHORS Jain.L.R. Actinobacteria. Gordoniaceae. University of TITLE Direct Submission rajasthan (11902589) JOURNAL Submitted (22-JAN-2010) Department of Botany.D. Corynebacterineae. partial sequence Features Sequence Change Region Shown Customize View Analyze This Sequence LOCUS GU727686 1491 bp DNA linear BCT Run BLAST 20-MAR-2010 Pick Primers DEFINITION Gordonia lacunae strain CCC12 16S ribosomal RNA gene.1491 /organism="Gordonia lacunae" /mol_type="genomic DNA" /strain="CCC12" /db_xref="taxon:417102" /country="India" rRNA <1.S.S. Srivastava. FEATURES Location/Qualifiers source 1.

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