Lab report 2 & 3 Microbial Genetic: Genomic DNA extraction of agarose gel electrophoresis and DNA quantification

Group member 1. Miss Kanoksachinut Ngeonsudja. 2. Miss Phratchaya Seeladlao. 3. Miss Phatamaporn Sooksai. ID: 5231105036 ID: 5231105037 ID: 5231105039

Objectives 1. 2. 3. 4. 5. To explain the basic of genomic DNA extraction. To practice the genomic DNA extraction technique. To explain the function of chemical uses in genomic DNA extraction. To practice the molecular genetics equipments. To assess the quality and quantity of genomic DNA by agarose gel electrophoresis and UV spectrophotometry.

Material & Equipments: Part I: Genomic DNA extraction from Pleurotus oatreatus on potato dextrose agar (PDA) Extraction buffer (200 mM)