Dye Solubility Test In this test, when an emulsion is mixed with a water soluble dye such as amaranth and
observed under the microscope, • if the continuous phase appears red- then it means that the emulsion is o/w type .
if the scattered globules appear red and continuous phase colorless- then it is w/o type. (Fig 1)
it will remain stable as water is the dispersion medium
if it is diluted with oil. the emulsion will break as oil and water are not miscible with each other.
If the emulsion is o/w type and it is diluted with water.Dilution test In this test the emulsion is diluted either with oil or water. (Fig 2)
Fig 2: Dilution Test for oil in water emulsion
Conductivity Test This test is based on the basic principle that water is a good conductor of electricity. this test will be positive as water is the external phase (Fig 8)
Fig 8: Conductivity test for oil in water emulsion and water in oil emulsion
incase of o/w emulsion .
Fluorescence Test If an emulsion on exposure to ultra-violet radiations shows continuous florescence under microscope. then it is Oil in o/w type. Staining Test A small amount of emulsion is mixed with scarlet red or methyl blue and examined under
.it is W/O emulsion. If there is no change in color . if it shows only spotty fluorescence.Cobalt Chloride Test When a filter paper soaked in cobalt chloride solution is added to an emulsion and dried If it turns pink then it is O/W emulsion. then it is w/o type .
If dispersed globules appear red. it is W/O type emulsion as scarlet red is soluble in water but not in oil.
.microscope. it is O/W type emulsion as scarlet red is soluble in oil but not in water. The test may fail if ionic emulsifiers are present . If dispersed globules appear blue.