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Biotechnology Procedures and Experiments Handbook$ VRP

Biotechnology Procedures and Experiments Handbook$ VRP

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Published by VRampriya
Biotechnology is one of the major new technologies of the twenty-first century that covers multi-disciplinary issues, including DNA techniques, cloning, genetics, and the application of microbiology to the production of goods. It continues to revolutionize the treatments of many diseases, and it is used to deal with environmental solutions. The Biotechnology Procedures and Experiments Handbook provides practicing professionals and biotechnology students over 150 applied, up-to-date laboratory techniques and experiments related to modern topics such as recombinant DNA, electrophoresis, stem cell research, genetic engineering, microbiology, tissue culture, and more. Each lab technique Includes: 1) a principle, 2) the necessary reagents, 3) a step by step procedure, and 4) a final result. Also included is a section that shows how to avoid potential pitfalls of a specific experiment. The book is accompanied by a CD-ROM containing simulations, MATLAB applications, videos, white papers, and other relevant material to biotechnology. Features *Presents a descriptive overview of each subject prior to the experiment or technique *Covers DNA, microbiology, genetic engineering, tissue culture (plant/animal), etc. *Sequentially links experiments to provide a project approach to biotechnology *Accompanied by a CD-ROM containing simulations, figures, white papers, Web sites and numerous other resources. Separate instructors' CD available upon adoption Brief Table Of Contents 1. General Instruction and General Laboratory Methods 2.Tools of Techniques in Biological Studies 3.Biochemistry 4. Entomology 5. Electrophoresis 6. Microbiology 7. Cell Biology and Genetics 8. Molecular Biology 9. Tissue Culture Techniques. Appendix A. Units and Measures. Appendix B. Chemicals or Reagents Required for Molecular Biology Experiments. Appendix C. Reagents Required for Tissue Culture Experiments Appendix C. Chemicals Required for Microbiology Experiments. Index.
Biotechnology is one of the major new technologies of the twenty-first century that covers multi-disciplinary issues, including DNA techniques, cloning, genetics, and the application of microbiology to the production of goods. It continues to revolutionize the treatments of many diseases, and it is used to deal with environmental solutions. The Biotechnology Procedures and Experiments Handbook provides practicing professionals and biotechnology students over 150 applied, up-to-date laboratory techniques and experiments related to modern topics such as recombinant DNA, electrophoresis, stem cell research, genetic engineering, microbiology, tissue culture, and more. Each lab technique Includes: 1) a principle, 2) the necessary reagents, 3) a step by step procedure, and 4) a final result. Also included is a section that shows how to avoid potential pitfalls of a specific experiment. The book is accompanied by a CD-ROM containing simulations, MATLAB applications, videos, white papers, and other relevant material to biotechnology. Features *Presents a descriptive overview of each subject prior to the experiment or technique *Covers DNA, microbiology, genetic engineering, tissue culture (plant/animal), etc. *Sequentially links experiments to provide a project approach to biotechnology *Accompanied by a CD-ROM containing simulations, figures, white papers, Web sites and numerous other resources. Separate instructors' CD available upon adoption Brief Table Of Contents 1. General Instruction and General Laboratory Methods 2.Tools of Techniques in Biological Studies 3.Biochemistry 4. Entomology 5. Electrophoresis 6. Microbiology 7. Cell Biology and Genetics 8. Molecular Biology 9. Tissue Culture Techniques. Appendix A. Units and Measures. Appendix B. Chemicals or Reagents Required for Molecular Biology Experiments. Appendix C. Reagents Required for Tissue Culture Experiments Appendix C. Chemicals Required for Microbiology Experiments. Index.

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Categories:Types, Research, Science
Published by: VRampriya on Sep 01, 2011
Copyright:Attribution Non-commercial

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01/26/2015

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Drosophila, like other animals, requires an optimum temperature for its survival,
growth, and breeding (20°–25°C). The temperature around and above 31°C
makes the flies sterile and reduces the oviposition; it may also result in death.
At lower temperatures, the life cycle is prolonged and the viability may be
impaired.

The routinely used food media for the maintenance of Drosophila is “cream
of wheat agar” medium.

The ingredients of this media for preparing culture bottles are as follows:

1.Distilled water – 1000 mL

2.Wheat flour (rava) – 100 gm

3.Jaggery – 100 gm

4.Agar agar – 10 gm

5.Propionic acid – 7.5 mL

6.Yeast granules.

Wheat flour and jaggery are boiled in distilled water until a paste isformed.
To that, agar agar and yeast granules are added after cooling. Propionic acid
is added to avoid fungal infection of the medium.

Heat-sterilized bottles should be used for preparing cultures. Similarly, ste-
rilized cotton has to be used to plug the bottles. As the condition of the medium
deteriorates with time, the flies have to be transferred from the old to new
culture medium at least once in 3 weeks.

Whenever the flies have to be analyzed, either for routine observations or
for experiments, they have to be anaesthetized to make them inactive. The
procedure is to transfer the flies from the media bottle to another empty wide-
mouthed bottle, referred to as an etherizer. The mouth of this bottle is covered
with a stopper and sprayed with ether. It takes about a minute to anaesthetize
the flies. After this, the flies are transferred to a glass plate for observation
under a stereo zoom microscope. If the etherized flies revive before the
completion of the observation, they have to be re-etherized using a re-etherizer
(ether-soaked filter paper fitted in a petriplate, which has to be placed over
the flies on the glass plate). The overetherized flies will have their wings and
legs extended at right angles to the body, and such flies are considered to be
dead.

The flies should be handled with a fine painting brush. In the process of
handling the flies, care should be taken not to damage them. The flies should
be discarded after observation.

CELL BIOLOGY AND GENETICS 343

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