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emulsified sputum mixed with antiserum gives capsule swelling (Quellung reaction) -peritoneal exudates can also be used
pneumoniae Quellung (capsular swelling) reaction can be used to demonstrate the presence of a specific capsular type of the bacterium.
-BAP in CO2 or candle jar; blood culture
--Streptococcus pneumoniae A mucoid strain on blood agar showing alpha hemolysis (green zone surrounding colonies). Note the zone of inhibition around a filter paper disc impregnated with optochin. Viridans streptococci are not inhibited by
Animals die in 18-48 hours; heart blood gives pure culture; sensitive but seldom used because of the need to maintain a mouse colony.