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Published by: Bidisha Ghosh on Oct 14, 2011
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In vitro Assessment of Antimicrobial Activity of some Ornamental Flowers

Presented by: Bidisha Ghosh 8th Sem, B.Tech, Biotechnology, SITM

Under the supervision of: Mr. Neeraj Mishra Asst. Prof. & Head (Dept. of Biotechnology), SITM

000 people/day Just 4yrs after the start of mass-producing penicillin in 1943 resistant microbes began appearing Development of resistance to common antibiotics in all major pathogens are reported These negative health trends necessitated a search for new antimicrobial substances from other sources including plants.Infectious disease & traditional drug problems Leading cause of premature deaths. killing almost 50. .

Use of flowers as medicine 50% of pharmaceuticals originate from plants Most plants contain bioactive compounds that have good antimicrobial activity Plant antimicrobials are not associated with side effects Some flowers with reported antimicrobial activity: Chrysanthemum Jasmine Poppy Rosemary Thyme .

Objective of project Evaluating antimicrobial activity of water & alcohol extracts of 4 flowers against 5 microorganism by Agar Well Diffusion Method. .

Flowers used Cyme rose (Rosa indica) Lotus (Nelumbo nucifera) Orange Marigold (Tagetes erecta) Yellow Red Marigold (Tagetes patula) .

water Ran in soxhlet (4 hrs at 100OC) Alcohol extract 5 g flower + 100 ml alcohol agitated for 8 hrs in a wrist-action shaker alcohol evaporated without heat & residue mixed with 100 ml of dist water .Preparation of flower extracts Water extract 5 g flower + 100 ml dist.

Microorganisms analyzed Bacillus subtilis Staphylococcus aureus Escherichia coli Pseudomonas aeruginosa Candida albicans .

Analysis of antimicrobial activity Method: Agar Well Diffusion Method Media: Muller Hinton agar Parameter: Zone of Inhibition Measuring the inhibition zone .

Antimicrobial activity against Staphylococcus aureus Water extract Alcohol extract .

Candida albicans showed the highest resistance & was susceptible only to Orange Marigold & Yellow Red Marigold. .Graphical representation of antimicrobial activity with water extract 12 inhibition zone(in mm) 10 8 6 4 2 0 Bacillus subtilis Staphylococcus aureus Escherichia coli Pseudomonas aeruginosa Tagetes patula Candida albicans Rosa indica Nelumbo nucifera Tagetes erecta Control Orange Marigold showed activity against all five microorganisms Rose gave the highest inhibition.

Graphical representation of antimicrobial activity with alcohol extract 12 inhibition zone (in mm) 10 8 6 4 2 0 Bacillus subtilis Rosa indica Staphylococcus aureus Nelumbo nucifera Escherichia coli Tagetes erecta Pseudomonas aeruginosa Candida albicans Control Tagetes patula Yellow Red Marigold showed very high activity against all five organism Rose also showed strong inhibiting capability Most resistant microorganism was again Candida albicans .

Conclusion Flower extracts have great potential as antimicrobial compounds Rosa indica & Tagetes patula showed maximum antimicrobial activity These flowers can be used to discover bioactive compounds that may serve as leads for development of new pharmaceuticals .

Neeraj Mishra. Director of Department of Biotechnology  All the faculty of the Dept.. SITM.. Biotechnology. Biotechnology. SITM.Acknowledgements  Mr. . & Head of the Dept.  My parents. Prof. Asst.  Dr. Vinod Bihari.

Thank You .

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