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SynGene GeneTools - File version: 3.08.01 - Serial No.

14141*11717*labfo*mpcsdq
SynGene Laboratories This heading can be changed by the user.

Image document data

File C:\Documents and


Settings\user\Desktop\
Warin\HMGCOAALL1.s
gd
Created by User
Generated on Wednesday February 27 2008 2:00:31 PM
ID 1204095631

Black:0 White:3519 Gamma:1.00


Properties

User Field 1
User Field 2
User Field 3
User Field 4
User Field 5

Capture
Digitizer G:BOX Chemi HR
(MOT) - Chemi
Sample
Camera Factory no filter no lights motorised lens
configuration
Settings GeneSnap
version 7.01.02
Exposure Time:
0.100 seconds
Frame
accumulation: Off
(Stop on
saturation: Off)
Darkroom camera
filter: EtBr/UV
Darkroom lighting:
Transilluminator
EDR: Off
Camera
Resolution: 1.38
MPixel (1x1 bin)
Camera Serial ID:
Q18677

User: User Created: Wednesday, February 27, 2008 2:27:31 PM ID : 1330845406


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SynGene GeneTools - File version: 3.08.01 - Serial No. 14141*11717*labfo*mpcsdq
SynGene Laboratories This heading can be changed by the user.

GrabberChain
Image
resampler
StepX 1
StepY 1
Quantage
Dynamic Range
Extender
Bypass -1
Equalize 0
ExposureRatio
2
Exposures
4
GaussianEqualiza
tion 0
MaximumIntensity
Fraction 0.9
MinimumIntensity
Fraction 0.1
MinimumValidCou
nt 1000
Type 2/4
Darkroom
CurrentFilterID
14
CurrentFilterPositi
on -1
FBLens 0
FocusPosition
-1
IrisFNumber
-1
ZoomFocalLength
-1
Camera
Calibration
bBackgndCorr
1
bStarfldCorr
1
corCameraID
"Q18677"
corFilePath
"cq.cor"
QCam
AbsoluteOffset
0
BayerToRGB
4
Capture8Bits
0
ExposureTime
0.1
Gain 1
GainBlue
-1
GainGreen
-1
GainRed-1
binningMode
1
cameraType
7

User: User Created: Wednesday, February 27, 2008 2:27:31 PM ID : 1330845406


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SynGene GeneTools - File version: 3.08.01 - Serial No. 14141*11717*labfo*mpcsdq
SynGene Laboratories This heading can be changed by the user.

Document history
Date Author
Wednesday February 27 2008 2:27:31 PM User
Integration parameters
Baseline correction method LOWEST SLOPE
Baseline correction offset YES
Color filtration options NO FILTER
Smoothing SAVITSKY-GOLAY order 2 width 3
Type Fluorescence
Image dimensions 1392 x 1040
Electrophoresis direction Down
Minimum peak height 3
Minimum peak width (pixels) 7
Minimum peak area 1

Track 1

User: User Created: Wednesday, February 27, 2008 2:27:31 PM ID : 1330845406


Signature :
SynGene GeneTools - File version: 3.08.01 - Serial No. 14141*11717*labfo*mpcsdq
SynGene Laboratories This heading can be changed by the user.

Profile height

2000

1400

800

200

0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Rf distance down track
Profile height

2200

3
4 7
2 10
1
Rf distance down track

8 11
9
56 12 13 14

0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9

Track 1
Number Mol. weight Height Raw vol.
1 0.00 945.233 992291.69
2 0.00 1020.486 983789.81
3 0.00 1263.683 1370056.50
4 0.00 1195.398 1247657.13
5 0.00 207.090 169205.17
6 0.00 227.329 165766.34
7 0.00 1191.705 1352304.25
8 0.00 310.848 555324.88
9 0.00 260.992 222308.56
10 0.00 1017.913 2024172.50
11 0.00 305.031 518440.94
12 0.00 198.375 163416.30
13 0.00 206.741 223899.30
14 0.00 241.795 229798.34

User: User Created: Wednesday, February 27, 2008 2:27:31 PM ID : 1330845406


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SynGene GeneTools - File version: 3.08.01 - Serial No. 14141*11717*labfo*mpcsdq
SynGene Laboratories This heading can be changed by the user.

Track 2
Profile height

2000

1400

800

200

0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Rf distance down track
Profile height

2200

Rf distance down track

89 10 14
4 6 7 13
1112
12

0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9

Track 2
Number Mol. weight Height Raw vol.
1 0.00 144.220 271377.34
2 0.00 145.184 146724.39
3 0.00 1147.073 1255023.38
4 0.00 216.634 297925.38
5 0.00 1482.349 2117007.75
6 0.00 232.981 223353.61
7 0.00 250.043 276922.66
8 0.00 268.665 255603.73
9 0.00 277.008 345126.31
10 0.00 290.083 274773.06
11 0.00 217.192 318099.38
12 0.00 240.711 226161.91
13 0.00 245.879 166512.67

User: User Created: Wednesday, February 27, 2008 2:27:31 PM ID : 1330845406


Signature :
SynGene GeneTools - File version: 3.08.01 - Serial No. 14141*11717*labfo*mpcsdq
SynGene Laboratories This heading can be changed by the user.

14 0.00 298.511 249904.66


Track 3
Profile height

2000

1400

800

200

0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Rf distance down track
Profile height

2200

Rf distance down track

15
7 8 9 1011 12 13 14
4 5
1 2

0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9

Track 3
Number Mol. weight Height Raw vol.
1 0.00 214.773 422314.66
2 0.00 231.206 267116.72
3 0.00 1163.996 1601397.88
4 0.00 253.676 241161.39
5 0.00 278.494 349070.47
6 0.00 1363.826 2037000.00
7 0.00 296.333 384228.03
8 0.00 303.523 294378.44
9 0.00 322.321 243948.81
10 0.00 320.743 240949.17
11 0.00 341.821 348312.38
12 0.00 362.684 249280.80

User: User Created: Wednesday, February 27, 2008 2:27:31 PM ID : 1330845406


Signature :
SynGene GeneTools - File version: 3.08.01 - Serial No. 14141*11717*labfo*mpcsdq
SynGene Laboratories This heading can be changed by the user.

13 0.00 339.775 247932.83


14 0.00 307.396 409732.66
15 0.00 371.304 334851.50
Track 4
Profile height

2000

1400

800

200

0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Rf distance down track
Profile height

2200
7

11

Rf distance down track

17
6 13 14 15 16
2 3 45 8 9 10 12
1

0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9

Track 4
Number Mol. weight Height Raw vol.
1 0.00 241.200 221575.81
2 0.00 254.575 433529.78
3 0.00 288.941 243489.33
4 0.00 286.627 543253.50
5 0.00 314.115 415504.94
6 0.00 346.509 484026.97
7 0.00 1976.070 2670709.00
8 0.00 298.704 244711.45
9 0.00 307.334 380138.31
10 0.00 326.690 416322.00

User: User Created: Wednesday, February 27, 2008 2:27:31 PM ID : 1330845406


Signature :
SynGene GeneTools - File version: 3.08.01 - Serial No. 14141*11717*labfo*mpcsdq
SynGene Laboratories This heading can be changed by the user.

11 0.00 1583.915 2225874.25


12 0.00 329.674 335739.38
13 0.00 361.788 367697.84
14 0.00 402.392 749170.38
15 0.00 384.013 573710.56
16 0.00 362.641 584767.56
17 0.00 423.372 416155.78
Track 5
Profile height

2000

1400

800

200

0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Rf distance down track
Profile height

2200

7
8

Rf distance down track

15
6 9 1011 12 13
1 2 3 45 14

0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9

Track 5
Number Mol. weight Height Raw vol.
1 0.00 294.806 277678.31
2 0.00 301.248 594952.31
3 0.00 325.239 260266.02
4 0.00 310.719 330992.28
5 0.00 342.251 273141.50
6 0.00 400.599 466047.22

User: User Created: Wednesday, February 27, 2008 2:27:31 PM ID : 1330845406


Signature :
SynGene GeneTools - File version: 3.08.01 - Serial No. 14141*11717*labfo*mpcsdq
SynGene Laboratories This heading can be changed by the user.

7 0.00 1933.007 2602491.75


8 0.00 1872.704 2929437.75
9 0.00 376.298 413331.50
10 0.00 386.813 503490.19
11 0.00 404.726 500802.03
12 0.00 395.665 619120.69
13 0.00 410.054 364745.78
14 0.00 338.414 367111.41
15 0.00 441.764 346023.56
Track 6
Profile height

2000

1400

800

200

0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0
Rf distance down track
Profile height

2200 10

Rf distance down track

3 6 11 12 14
1 2 45 8 9 13

0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0

Track 6
Number Mol. weight Height Raw vol.
1 0.00 344.619 421244.78
2 0.00 344.109 444972.47
3 0.00 434.500 358623.00
4 0.00 345.797 747055.63

User: User Created: Wednesday, February 27, 2008 2:27:31 PM ID : 1330845406


Signature :
SynGene GeneTools - File version: 3.08.01 - Serial No. 14141*11717*labfo*mpcsdq
SynGene Laboratories This heading can be changed by the user.

5 0.00 378.217 382886.50


6 0.00 394.034 316126.63
7 0.00 1837.906 2403918.50
8 0.00 344.148 262173.47
9 0.00 372.801 460800.13
10 0.00 2089.154 3185489.25
11 0.00 412.117 640268.06
12 0.00 426.304 634943.44
13 0.00 378.371 365629.44
14 0.00 424.965 355946.66
Track 7
Profile height

2000

1400

800

200

0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0
Rf distance down track
Profile height
7

2200
8

Rf distance down track

10
5 9 14
6 1112 13
1 2 3 4

0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0

Track 7
Number Mol. weight Height Raw vol.
1 0.00 330.920 291037.25
2 0.00 338.942 262897.38
3 0.00 326.598 294799.63

User: User Created: Wednesday, February 27, 2008 2:27:31 PM ID : 1330845406


Signature :
SynGene GeneTools - File version: 3.08.01 - Serial No. 14141*11717*labfo*mpcsdq
SynGene Laboratories This heading can be changed by the user.

4 0.00 307.459 274184.47


5 0.00 439.154 1254271.88
6 0.00 384.751 317799.53
7 0.00 2322.408 3147537.00
8 0.00 2024.723 3006358.75
9 0.00 426.358 581738.69
10 0.00 498.047 972006.81
11 0.00 392.032 268252.03
12 0.00 400.517 306570.06
13 0.00 361.673 333193.75
14 0.00 410.707 347528.06
Track 8
Profile height

2000

1400

800

200

0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Rf distance down track
Profile height

2200

8
4

Rf distance down track

11
7 9 10 12 13 1415
1 23 56

0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9

Track 8
Number Mol. weight Height Raw vol.
1 0.00 259.515 260789.58
2 0.00 302.424 919503.63

User: User Created: Wednesday, February 27, 2008 2:27:31 PM ID : 1330845406


Signature :
SynGene GeneTools - File version: 3.08.01 - Serial No. 14141*11717*labfo*mpcsdq
SynGene Laboratories This heading can be changed by the user.

3 0.00 312.240 232458.67


4 0.00 1597.980 2149778.75
5 0.00 289.904 218510.28
6 0.00 293.884 243920.02
7 0.00 323.876 307954.91
8 0.00 1740.585 2432737.25
9 0.00 358.414 319916.72
10 0.00 382.705 418122.91
11 0.00 425.472 872820.81
12 0.00 366.055 774783.06
13 0.00 384.716 395159.81
14 0.00 363.768 963405.88
15 0.00 386.312 288989.34
Track 9
Profile height

2000

1400

800

200

0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Rf distance down track
Profile height

2200

Rf distance down track

9 10 11 14
7 8 12 13
1 2 34

0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9

Track 9
Number Mol. weight Height Raw vol.

User: User Created: Wednesday, February 27, 2008 2:27:31 PM ID : 1330845406


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SynGene GeneTools - File version: 3.08.01 - Serial No. 14141*11717*labfo*mpcsdq
SynGene Laboratories This heading can be changed by the user.

1 0.00 251.100 221257.45


2 0.00 242.344 246917.09
3 0.00 248.743 389977.75
4 0.00 283.805 308869.59
5 0.00 1467.087 1779501.38
6 0.00 1815.287 2704314.00
7 0.00 328.548 270833.41
8 0.00 340.707 212988.03
9 0.00 382.475 235651.44
10 0.00 366.093 624948.63
11 0.00 377.269 515510.06
12 0.00 328.400 370588.94
13 0.00 367.415 820790.25
14 0.00 403.014 331083.25
Track 10
Profile height

2000

1400

800

200

0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Rf distance down track
Profile height

2200

Rf distance down track

12 1314 1 617
15
9 1011
6 8
23 5
1

0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9

Track 10

User: User Created: Wednesday, February 27, 2008 2:27:31 PM ID : 1330845406


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SynGene GeneTools - File version: 3.08.01 - Serial No. 14141*11717*labfo*mpcsdq
SynGene Laboratories This heading can be changed by the user.

Number Mol. weight Height Raw vol.


1 0.00 130.174 258560.81
2 0.00 183.222 243735.16
3 0.00 196.085 205091.88
4 0.00 1591.210 1753836.88
5 0.00 185.524 283597.28
6 0.00 231.561 788780.69
7 0.00 1781.337 2280620.75
8 0.00 254.915 260464.28
9 0.00 274.674 652762.88
10 0.00 300.257 778415.19
11 0.00 323.637 438998.63
12 0.00 320.068 706610.06
13 0.00 326.952 226700.61
14 0.00 350.522 593141.38
15 0.00 343.017 276171.69
16 0.00 362.788 249510.86
17 0.00 376.035 264683.84
Track 11

User: User Created: Wednesday, February 27, 2008 2:27:31 PM ID : 1330845406


Signature :
SynGene GeneTools - File version: 3.08.01 - Serial No. 14141*11717*labfo*mpcsdq
SynGene Laboratories This heading can be changed by the user.

Profile height

2000

1400

800

200

0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Rf distance down track
Profile height

2200

5
2

Rf distance down track

7 8 9 1213
6 10 11
3 4
1

0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9

Track 11
Number Mol. weight Height Raw vol.
1 0.00 133.505 256654.59
2 0.00 1191.264 1253111.13
3 0.00 188.645 214606.59
4 0.00 214.621 261395.27
5 0.00 1240.903 1709672.88
6 0.00 250.060 153640.67
7 0.00 333.131 415661.28
8 0.00 301.037 346688.97
9 0.00 331.751 160303.64
10 0.00 289.548 279472.72
11 0.00 295.257 164058.42
12 0.00 346.985 709048.88
13 0.00 390.445 346327.41
Track 12

User: User Created: Wednesday, February 27, 2008 2:27:31 PM ID : 1330845406


Signature :
SynGene GeneTools - File version: 3.08.01 - Serial No. 14141*11717*labfo*mpcsdq
SynGene Laboratories This heading can be changed by the user.

Profile height

2000

1400

800

200

0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Rf distance down track
Profile height

2200

Rf distance down track

6 112
101 1516
5 78 9 13 14
1 3

0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9

Track 12
Number Mol. weight Height Raw vol.
1 0.00 173.102 197437.06
2 0.00 1385.360 1375628.25
3 0.00 163.045 154593.55
4 0.00 1824.946 2064618.50
5 0.00 247.158 170177.91
6 0.00 316.128 220722.75
7 0.00 283.757 234896.45
8 0.00 293.290 279740.44
9 0.00 285.359 176548.98
10 0.00 304.085 248046.38
11 0.00 309.574 192691.27
12 0.00 315.919 196505.52
13 0.00 285.557 213513.23
14 0.00 280.849 154139.63

User: User Created: Wednesday, February 27, 2008 2:27:31 PM ID : 1330845406


Signature :
SynGene GeneTools - File version: 3.08.01 - Serial No. 14141*11717*labfo*mpcsdq
SynGene Laboratories This heading can be changed by the user.

15 0.00 330.665 585015.56


16 0.00 366.672 282982.03
Track 13
Profile height

2000

1400

800

200

0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Rf distance down track
Profile height

2200

Rf distance down track

15
89 10 1112 13 14
5 7
12 4

0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9

Track 13
Number Mol. weight Height Raw vol.
1 0.00 130.229 158148.13
2 0.00 152.720 148392.31
3 0.00 1263.610 1253963.38
4 0.00 164.835 291273.09
5 0.00 195.598 284590.06
6 0.00 1713.136 2071199.13
7 0.00 217.463 147704.55
8 0.00 235.217 162770.02
9 0.00 238.414 279202.75
10 0.00 276.619 225777.78
11 0.00 279.814 154844.63

User: User Created: Wednesday, February 27, 2008 2:27:31 PM ID : 1330845406


Signature :
SynGene GeneTools - File version: 3.08.01 - Serial No. 14141*11717*labfo*mpcsdq
SynGene Laboratories This heading can be changed by the user.

12 0.00 292.556 341837.22


13 0.00 263.703 213730.36
14 0.00 302.465 349122.56
15 0.00 329.521 251945.58
Track 14
Profile height

2000

1400

800

200

0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Rf distance down track
Profile height

2200

Rf distance down track

5 6 1 011
78 9
3 4
2
1
0

0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9

Track 14
Number Mol. weight Height Raw vol.
1 0.00 66.836 114285.02
2 0.00 137.581 100975.70
3 0.00 169.365 103153.47
4 0.00 189.411 130706.80
5 0.00 224.270 187082.70
6 0.00 246.303 208020.92
7 0.00 219.933 105327.93
8 0.00 225.871 243632.84
9 0.00 257.101 335834.56

User: User Created: Wednesday, February 27, 2008 2:27:31 PM ID : 1330845406


Signature :
SynGene GeneTools - File version: 3.08.01 - Serial No. 14141*11717*labfo*mpcsdq
SynGene Laboratories This heading can be changed by the user.

10 0.00 261.253 249232.83


11 0.00 278.308 208397.64
Molecular weight calibration details
Curve type Log piecewise linear
Distance measurement From Rf baseline
Propagation method Interpolate between standards
No molecular weight standards are defined !
Quantity calibration details
Curve type Linear through origin (single standard value)
Calibrate All tracks to a single curve
Units
No quantity calibrations are defined !

User: User Created: Wednesday, February 27, 2008 2:27:31 PM ID : 1330845406


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