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2011

4 :

(Lipton Linea tea) HPLC
:
A : 110125

: 18/11/2011
: 25/11/2011

HPLC
1.)

/ (HPLC High Performance / Pressure
Liquid Chromatography).
/
.

2.)
2.1 GC HPLC
2 ( ) 2
..
(stationary) (mobile) , .
, .
. (
o Tswett .)

GC .

LC

- .
- PLC .

PLC GC

- A
- , ( )
, .

- , .
2.2 HPLC
.1 HPLC.

. 1

1)
2)
3)
4)
5)
6)
6)
7)

8) ( )
9) ()
10)
11) (PC,,)
12)

2.3 HPLC

- 500 ml
-
.
2 HPLC :
.
a) I (1 - Solvent) 1 .

b1) ( 2 - Solvent A,B) 2


, -

b2) ( 4 - Solvent A,B,C,D)) 1


, 1 4 .


14 6000 psi ( 400 bar) .
T ( 2004) : UPLC (Ultra Performance liquid Chromatography)
15000 psi ( 1000 bar).
T
( ).

( )

.2


. 1-500 L
(.2). 2
.

.3 PLC

2-3 mm.
10 100 cm
GC.
(., / )
HPLC (, , .)
( ..).
/

.

- A

(.4).
.
(190-600 nm) .M

. Beer- Lambert
.
:
a)A (RI refraction index)
b)
c) ,FID,..
.4 HPLC
3

2.4 HPLC
HPLC (,,).O
(,,)
. ,
.


( )
.

HPLC
X ,
( , )
2 :
a) , ()
( ) , ..
b) X , ( ,
, , ..)
(, .) . : X (
), , ( ,
), (
)


a) ( ) tRX
( ) tRS .
, ( 1
) , 2
(
)
b) H :

- ()
- ,

, ( ) .

.

,
( ) .

3.)
,,,,,,,,,,

, , , , , ,
Lipton Linea Tea HPLC.
3.1

15 min ( )
.
( 0,45 nm) .
CH3OH 34% H20 64%.
3.2
Hewlett- Packard 1100 HPLC, Macherey Nagel
Nucleosil 100 S C18 240 * 4,6 mm.O
274 nm. H 12 min
(t = 0 )
,
4

. 5 (CH3OH 34% H20 64%)


270 nm.

200 -700 nm ( )
100 l 20 l.

4.)

.6 1.

. 6 Lipton Linea Tea

1.
,

No 14( 1) 6,112 min, 8810,86133


mAU * s 753,83771 mAU 25,6805% (Area %)
5
7 2 8.

.7

2:
,

6
,

.10 (calibration curve)

:
0,16255 mg/ml.

1) Skoog,Holler,Nieman (2005) ,
2) http://www.waters.com/waters/nav.htm?cid=10048919&locale=en
3.). . (2008) ,
,
4)Biosciences Sheffield, www.schu.ac.uk