NaSc1

Lecture 2

Microscopy

Very important in the study cells Started when man discovered that objects could be magnified (early 17th century) More advanced types emerge

Concepts

Magnification Resolution Working Distance Depth of Field

http://images.search.yahoo.co m/images/undefined

Types of microscopes
1)

Light Microscopes Bright-field microscope Dark-field microscope Phase-contrast microscope

http://www.digitalsmicroscope .com/compound-lightmicroscope-labeled-3/

Bright Field

Dark Field

Phase Contrast

www.wikipedia

Dissecting Microscope

http://hairstyle-update.onsugar.com/ Compound-Microscope-Parts-17733346

http://www.digitalsmicroscope.com/wpcontent/uploads/2011/08/CompoundLight-Microscope-Labeled-3.jpg

Types of microscopes
2) Fluorescence microscope

Light microscope (UV light) Use fluorescent dyes (acridine orange or fluorescein) to directly stain samples

*Direct counts

Types of microscopes
3) Confocal Laser Scanning microscope

3D Flourescent images

dornsife.usc.edu

http://emu.arsusda.gov /cfm.html

Electron Microscope

http://avonapbio.pbworks.com/w/pag e/9429544/Types

www.sciencephotogallery.com

SEM, Human Chromosomes, magnification x7000
http://www.psmicrographs.co.uk/

SEM of Human Red and White Blood Cells, magnification x5167
http://www.psmicrographs.co.uk/

Sources

Miller, S.A. & Harley J.B. 1999. Zoology fourth ed. WCB/McGraw-Hill USA.

http://www.digitalsmicroscope.com/compoun d-light-microscope-labeled-3/

A. Label the parts of the diagram
1. 8.

2.

9.

3. 4. 5.

10..

11.

6. 7. 13..

12.

A. Answers:
1.

Cell membrane

9. Cytoplasm

2.
3.

Lysosome
Nucleus

10. Rough er
11. Smooth er

4.
5. 6. 7.

Nucleolus
Vacuole Mitochondrion

12. Ribosomes
13. Golgi body

Nuclear membrane

Zool Lab: Exercise 3

BLOOD CELLS

SIMPLE COLUMNAR

CILIATED COLUMNAR

SQUAMOUS EPITHELIUM CELL

SMOOTH MUSCLE CELLS

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