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Enz02 Kinetics

Enz02 Kinetics

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Published by: Ruqaiya Samad on Dec 31, 2011
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Enzyme Kinetics

Enzyme activity
Student A

Score
0

Student B Student C

1 2 3 4 Exam Chapters

0 1 2 3 4 Substrate concentration

Increase the substrate concentration, observe the change of enzyme activity
Juang RH (2004) BCbasics

Invertase (IT) Non-reducing sugar Reducing sugars Reducing Power Sucrose HOCH2 O 1 2 IT Glucose + Fructose HOCH2 6 O 5 4 3 1 2 OH HOCH2 HOCH2 O OH HO H2O HOCH2 HOCH2 6 1 O 5 2 F 1 2 3 4 5 6 4 3 OH F HOCH2 HOCH2 O O HO-C-H H-C-OH H-C-OH H2-C-OH HO-C-H H-C-OH H-C-OH H2-C-OH Juang RH (2004) BCbasics HOCH2 O CHO H-C-OH H2C-OH C=O .

Increase Substrate Concentration 0 1 2 3 4 5 6 7 8 80 60 S + E P (in a fixed period of time) Product 40 20 0 0 2 4 6 8 Substrate Qmole) Juang RH (2004) BCbasics .

the production and consumption of the transition state proceed at the same rate. . So the concentration of transition state keeps a constant.Essential of Enzyme Kinetics Steady State Theory E+ S E S E +P Juang RH (2004) BCbasics In steady state.

Constant ES Concentration at Steady State S Concentration P ES E Reaction Time Juang RH (2004) BCbasics .

y) plot get hyperbolic curve. estimate Vmax 5) When y = 1/2 Vmax calculate x ([S]) Km 1 vo -1 Km 1 Vmax Vmax vo Juang RH (2004) BCbasics 1/2 Double reciprocal 1/S Km Direct plot S .An Example for Enzyme Kinetics (Invertase) 1) Use predefined amount of Enzyme E 2) Add substrate in various concentrations S (x M) 3) Measure Product in fixed Time (P/t) vo (y M) 4) (x.

0 1/v 1.92 1/S 4 2 1 0.16 (1) The product was measured by spectroscopy at 600 nm for 0.46 0.5 2.A Real Example for Enzyme Kinetics Data Substrate Product no Velocity Double reciprocal 1 2 3 4 [S] Absorbance v (Qmole/min) 0.35 1.56 1.0 1.40 0.36 0.25 0.21 0.08 1.0 0.80 2.42 0.0 v 0.0 -3.8 Juang RH (2004) BCbasics 0 0 1 2 [S] 0 -4 -2 0 1/[S] 2 4 .50 0.72 1.0 0.05 per Qmole (2) Reaction time was 10 min Double reciprocal Direct plot 1.5 1/v 2.

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