DNA extraction preparation

Laboratory Protocol

Solomon Lab 20 July 1994

θ θ θ θ θ θ θ pellet (with buffy coat) from serum preparation protocol DNA extraction buffer Two beige­top blood collection tubes Desktop centrifuge Pipette 18­gauge needle 50 ml plastic tube


Using the pipette draw up buffy coat from each of the four purple top tubes and pipette into a beige top tube

Some extra erythrocytes and serum drawn up is ok.

Pipette water into a second beige top tube and balance the two by volume.

Centrifuge for 20 minutes at a power of "4" on the dial

Label 50ml plastic tube with patient name, MRN, date, & nature of specimen

Using pipette carefully draw off buffy coats and pipette into a 50 ml plastic tube

DNA extraction buffer is in freezer above lab refrigerator

Pipette 15 ml of thawed DNA extraction buffer into 50 ml tube and store in refrigerator.

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