DNA extraction preparation

Laboratory Protocol

Solomon Lab 20 July 1994

Materials
θ θ θ θ θ θ θ pellet (with buffy coat) from serum preparation protocol DNA extraction buffer Two beige­top blood collection tubes Desktop centrifuge Pipette 18­gauge needle 50 ml plastic tube

Protocol

Using the pipette draw up buffy coat from each of the four purple top tubes and pipette into a beige top tube

Some extra erythrocytes and serum drawn up is ok.

Pipette water into a second beige top tube and balance the two by volume.

Centrifuge for 20 minutes at a power of "4" on the dial

Label 50ml plastic tube with patient name, MRN, date, & nature of specimen

Using pipette carefully draw off buffy coats and pipette into a 50 ml plastic tube

DNA extraction buffer is in freezer above lab refrigerator

Pipette 15 ml of thawed DNA extraction buffer into 50 ml tube and store in refrigerator.

Sign up to vote on this title
UsefulNot useful