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Lab Manual 2009

Lab Manual 2009

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Published by: Marrion Jules Sequitin Mendoza on Jan 28, 2012
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07/07/2013

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Dental plaque is the mass of bacteria in a matrix of organic material which

adheres tenaciously to the tooth surface. It is made up largely of protein and

polysaccharide which is partly derived from saliva and partly produced by the

organism . Plaque does not include food debris or exfoliated epithelial cells which

are only transient features.

Dental plaques are composed of about 20% precipitated salivary mucoid or

mucin and about 80% microorganisms.

Objectives:

1. To identify the presence of microorganisms in dental plaque

2. To know the different types of plaque.

Materials:

1. Clean glass slides

2. Reagents for Gram¶s and Negative staining procedure

3. Toothpick

Procedure:

1. Collect plaque from the enamel and dentin with the use of a toothpick.

2. Prepare a bacterial smear from the collected sample.

3. Perform Gram¶s and negative staining procedure.

4. Wash and dry stained smears.

5. Examine specimen under OIO.

6. Record your results and observations.

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Observations:

Review Questions:

1. What organisms make up the considerable bulk of the microbial flora of the

dental plaque?

________________________________________________________________________

________________________________________________________________________

________________________________________________________________________

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________________________________________________________________________

2. .How are plaque formed? ______________________________________________

________________________________________________________________________

________________________________________________________________________

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________________________________________________________________________

________________________________________________________________________

3. Name the bacterial species which are predominant in superficial enamel decay.

________________________________________________________________________

________________________________________________________________________

________________________________________________________________________

________________________________________________________________________

4. What are the three types of dental plaque? ________________________________

________________________________________________________________________

________________________________________________________________________

________________________________________________________________________

________________________________________________________________________

________________________________________________________________________

________________________________________________________________________

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Exercise No. _____

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