Total Protein concentration

Materials 1. Braford reagent 2. BSA std: 8ug, 4ug, 2ug, 1ug, 0.5ug, 0,25ug, 0.125ug, 0.0625ug 3. 96-well plate, flat bottom

Protocol 1. Braford reagent, make a 1:4 dilution in SDW 2. In eppendorf tube 300uL Braford reagend + 3uL BSA std + 3uL ddH2O + 3uL sample vortex, stand 5min, vortex 3. Add 200uL of each in 96well-plate 4. Read OD595nm

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