Cellular Respiration Introduction Cellular respiration is your body's way of breaking down food molecules (carbohydrates, proteins, and

fats) and making their stored energy available to the cell. Just like in plants, photosynthesis makes food and energy for in the form of glucose, animal cells like we have in our bodies, need to make ATP for energy. The first process of cellular respiration is called aerobic respiration. This process will happen when oxygen is present. There is also anaerobic respiration, which will happen in the absence of oxygen. Aerobic respiration can be broken down into four phases. These are Glycolysis, Transition Reactions, Kreb's Cycle and Electron Transport Chain. Investigate the effect of temperature on the cellular respiration, and the relationship between dependent and independent variables. Hypothesis The rate of cellular respiration will be greater in germinated peas than in dry peas.

respirometers. then take reading. Clean the work area. thermometer. masking tape. stopwatch. after everything is finished. dry peas. food coloring. find the volume of germinating peas. Next. water baths.germinating peas and bead. and set up all the material needed for that lab. Put non-absorbent cotton balls in each vial above the KOH and then add the peas and beads. absorbent cotton balls. non. Take additional reading every 5 minutes for 30 minutes. glass marking pen. Add a drop of food coloring to the exposed tip of each respirometer. Than begin the experiment by setting up two water baths. Record in the table. Equilibrate for 5 minutes. Methods First set up the vials with absorbent cotton balls. Allow the respirometers to equilibrate for 10 minutes.Materials Germinated peas. petroleum jelly. potassium hydroxide. pipet. Lower the into the water baths. Repeat these steps for the second set of peas and beads. and beads alone. paper towels. Place first set of vials in the room temperature water bath and the other set into the 10 degree water bath with ice. for all six vials. Results . one at room temperature and the other at 10 degrees Celsius. glass beads. graduated cylinder. nonabsorbent cotton balls. Drop 1 ml of KOH in the vial with absorbent cotton balls. ice.

7 0 Vials 1.1 0.9 8.3 -0.8 0.7 5 8.5 0.1 8.7 9 0.4 8.1 0.9 0.3 -0.1 0.9 0 0 0 7.5.6 0 -0.8 0.2.11 0.8 8.2 0.4 8.1 0 8.3 -0.7 0.12 -1 -2 -2 -2 0 0.5 9 0.7 0.2 25 8.4 8 8.1 4.6 -1.Table 1 Germinated Peas Reading Diff 0 8.4 -0.9 7. Diff Reading Diff Corr.1 0.3 -0.5 15 8.3 Temp (C Time 15 14 14 14 14 14 14 8 7 8 7 7 7 7 0.2 9.7 0.2 8.11 -1.4 -0.2 8.6 -1.7 7.5 0.5 8.8 0 8.1 0.5 -1.1 0.2 8.6 0.5 0.7 0 0 0 0 0 Dry peas and beads Beads only Corr.2 8.1 0.66 10 8.6 0 -0.2 8.7 8.7 0.8 0. Diff Reading Diff 8.9 0.4 -0.4 20 8.7 8.11 .9 0 5 10 15 20 25 30 8.4 -0.7 0.1 0.3 0.1 0.3 0 -0.1 0.1 30 7.4 8.4 0 -0.9 0.

Dry peas Question 1. According to your graph. . what happens to the rate of oxygen consumed by germinating peas over time? What does this indicate to you? The rate of oxygen consumption is constant. 2. Identify at least three of the controls. and describe the purpose of each.

What is the purpose of KOH in this experiment? The KOH drops absorbed the carbon dioxideand caused it to precipitate at the bottom of the vial and no longer able to effect the readings. 5. The water moved into the pipette because thevial and pipette were completely submerged into the bath. the water baths held a constant temperature. 3. the equilibration period was identical for all the respirometers. resulting in a decrease of gas in the pipette. While the peas underwent cellular respiration. 4. germinating or non-germinating. which reacted with the KOH inthe vial. consumes the most oxygen? Why? .First of all. 6. How did the KOH affect the water movement in the respirometer? It allows more water into the pipette. the volume of KOH was constant from vial to vial. Explain why water moved into the respirometer pipettes. Which of the two pea types. they consumed oxygen and released carbon dioxide. Than.

Anaerobic Prodices 2 ATP per glucose Aerobic Produces 32 ATP per glucose More efficient Produces ATP . Which end products contain the carbon atoms from glucose? The hydrogen atoms from glucose? The oxygen atoms from glucose? The energy stored in the glucose molecules? Carbon dioxide contains the carbon. glucose is broken down to form several end products. 8. 7. Plants use alcoholic while animals use lactic acid. 9. and ATP contains the energy.Germinating peas consume more oxygen because they are growing and are more active than non-germinating peas. What is fermentation? What are the two types of fermentation? What organisms use fermentation? Fermentation is a catabolic process that makes a limited amount of ATP from glucose without an electron transport chain and that produces an end-product such as ethyl alcohol or lactic acid. What was the effect of temperature on pea respiration? Warmer temperatures allow for the peas to respire at a faster rate. The two types of fermentation are alcoholic and lactic acid fermentation. water contains the hydrogen. During aerobic respiration. 10. both carbon dioxide and water contain the oxygen.

NADH. Water is formed c.11. 12. . Write the letter of the pathway that best fits each of the following processes. Electron Transport System 1. Glycolysis b. Carbon dioxide is given off b. The reaction of the pyruvate joining with CoA enzyme and NAD to produce acetyl CoA. What are the three pathways involved in the complete breakdown of glucose to carbon dioxide and water? What reaction is needed to join two of these pathways? What are the substrates and products of this reaction and where does it take place? The three pathways are glycolysis. the electron transport chain. and the Krebs Cycle. The acetyl CoA goes to the Krebs Cycle and NADH to the electron transport chain in the mitochondria. Krebs Cycle c. and carbon dioxide. 2. a.

. 7. Four of the ATPs are formed from substrate level and 32 from oxidative. Calculate the energy yield of glycolysis and cellular respiration per glucose molecule. FAD becomes FADH2 b. Distinguish between substrate-level phosphorylation and oxidative phosphorylation. 4. NADH becomes NAD+ c. 13. Cytochrome carriers c. 6. Pyruvate a. 8. Oxidative phosphorylation c. 5.3. Where does the energy for oxidative phosphorylation come from? 36 ATPs are formed per glucose moelcule. PGAL a.

For the cell to use. Write a short letter below. Energy is released in the form of ATP. 16. Prepare a system where you test respiration in a small animal and in the space provided below. Error Analysis: A lot of errors could have happened in this lab. beads. You have just performed an activity using plant seeds. the amounts of peas. Heart rate.14. slows down in cold temperature. The cotton balls were not cut in half. Timing could have been wrong. explaining cellular respiration to the substitute teacher. Your teacher has the flu and the only available substitute knows nothing about cellular respiration. KOH. Diffusion through membranes. 15. Air may have have got into of the vial via a leaky stopper or poorly sealed pipette. Cellular respiration is the process of oxidation. Name some other biological processes that are affected by temperature. Didn’t wait a minute after putting food coloring. and cotton may have been wrong. draw what that system would look like. You are given the responsibility to provide the substitute teacher with the important background information needed to explain this topic to the next class. The temperature of the baths may have changed. .

The non-germinating peas showed very little oxygen consumption while the germinating peas had a high rate of oxygen consumption. Respiration rate was also affected by temperature. The hypothesis. Oxygen consumption in the respirometers with germinating peas was greater than that in respirometers with non-germinating peas. Also the temperature did affect the rate . was in fact correct.Discussion and Conclusion: The lab and the results gained from this lab demonstrated many important things relating to cellular respiration. Respiration occurred at a faster rate in the respirometers in the warm water bath. the rate of cellular respiration will be greater in germinated peas than in dry peas.

the results were not correct.of cellular respiration. The germinated peas still had a higher rate of cellular respiration. but in the cold temperature the food coloring leaked out. Citations “AP Biology Lab 5 Cellular Respiration Activity” Ward’s Natural Science. 2004: 1-4. 2011. 2012 . The energy was converted to ATP faster. “What Affects the Heart Rate?”. Heart Monitors. Web.21 Feb. Sales & Customer Service. But because of all the error occurred during the lab. Print.

Cellular Respiration Lab Alyona Booth 2/21/2012 Per. 4 .

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