SCHOOL OF

BIOTECHNOLOGY

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SUBJECTS (Biochemistry) Code BC201 BC202 BC203 BC204 BC205 BC206 BC207 BC208 Subject Name Biochemistry Biochemistry Lab Bioorganic chemistry Food Biochemistry & Nutrition Food Biochemistry lab Instrumental Methods of Analysis Instrumental Methods of Analysis lab Biophysics BC201 BIOCHEMISTRY Credit : 4:0:0 Credit 4:0:0 0:0:2 4:0:0 4:0:0 0:0:2 4:0:0 0:0:2 4:0:0

Unit - I : Structure and Properties of Carbohydrates and Lipids Structure and properties of mono, di, oligo and polysaccharides, complex carbohydrates. Structure and properties of fatty acids, phospholipids, sphingolipids, glycolipids and steroids. Unit – II : Structure and Properties of Proteins and Nucleic Acids Structure and properties of amino acids, modified amino acids, peptides, proteins and conjugated proteins. Structure and properties of purines, pyrimidines, nucleosides, nucleotides, polynucleotides, ribonucleic acids, deoxy ribonucleic acids, nucleoprotein complexes. Unit-III : Metabolism of Lipids, Proteins and Nucleic Acids Biodegradation of proteins and nucleic acids. Biosynthesis and biodegradation of amino acids, fatty acids, cholesterol, purines and pyrimidines Unit – IV : Intermediary Metabolism and Bioenergetics Carbohydrate Metabolism: Glycolysis, TCA cycle, gluconeogenesis, pentose phosphate pathway - Urea Cycle – Overview of intermediary metabolism: Interconnections and regulation of metabolic pathways. Bioenergetics: redox biochemistry, energy rich compounds, respiratory chain, oxidative phosphorylation and triose phosphate cycle.

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Unit – V : Structure Function Relationship Structural and functional relationships in complex carbohydrates, conjugated proteins and conjugated nucleic acids. Text book: 1. Lehninger, AL, Nelson DL and Cox MM, Principles of Biochemistry References: th 1. Lubert Stryer, Biochemistry, 4 Edition, WH Freeman & Co., 2000. 2. Voet and Voet, Biochemistry, 2nd Edition, John Wiley & Sons Inc., 1995. 3. Murray, R.K., Granner, B.K., Mayes, P.A., Rodwell. V.W., Harper’s Biochemistry, Prentice Hall International.

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School of Biotechnology

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BC202 BIOCHEMISTRY LAB Credit : 0:0:2 Marks (50+50) 12 experiments will be notified by the HOD from time to time

BC203 BIO-ORGANIC CHEMISTRY Credit : 4:0:0

Unit – I Concepts in Organic Chemistry Stereochemistry – R,S notation – re-si faces – e,z isomerism- conformers- ethane – cyclopentane - reactivates- mechanisms of sn1 sn2 reactions, e1 e2 reactions – ester formation and hydrolysis, reaction rates - Hammond's postulate – h/d effects. Catalysis – general acid – base and covalent catalysis.

Unit - II Stereochemistry of Enzymatic Reactions Stereospecific enzymatic reactions – fumarase catalysed reactions – NAD dependent oxidation and reduction reactions - stereochemistry of nucleophilic reactions – chiral methyl group – chiral phosphate. UNIT – III Case Studies of Enzyme Structure and Mechanism The dehydrogenases – the proteases – ribonucleases – lysozyme- stability of proteins – stability – activity tradeoff. UNIT – IV Kinetics of Protein Folding Basic methods – two state kinetics – multistate kinetics – transition states in protein folding – 1h/2h exchange methods – folding of peptides. UNIT – V Folding Pathways & Energy Landscapes Folding of ci2 – nucleation condensation mechanism – folding of barnase – time resolution – insights from theory – optimization of folding rates – molecular chaperones. Text book 1. R Fersht, W.H. Freeman, Structure and Mechanism in Protein Science : A Guide To Enzyme Catalysis and Protein Folding;,1999. Reference books 1. H. Dugas, Bioorganic Chemistry ; Springer Verlag, 1999. 2. Fisher J and Arnold JRP Chemistry for Biologists”, VIVA Books Pvt Ltd, New Delhi 2001.

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BC204 FOOD BIOCHEMISTRY AND NUTRITION Credit : 4:0:0 Marks (40+60)

Unit I Glycolysis – Embden Meyerhof Pathway (EMP), Pentose phosphate shunt, TCA cycle, gluconeogenesis, urea cycle, inter connections of pathways, metabolic regulation, Bioenergetics – Electron transport chain, respiratory chain ATP cycle, energy rich compounds. Unit II Biosyntheses and degradation of fatty acids, and cholesterol - Biosyntheses and degradation of amino acids, peptides and proteins; Biosynthesis and degradation of purines, pyrimidines and nucleic acids.

Unit III Basic concept of nutrition – Importance of nutrition and dietetics - Assessment of nutritional status – energy value of carbohydrates, proteins and fats – determination of energy value – balanced diet – Recommended dietary intake – Acceptable dietary intake – Protein efficiency ratio – Net protein utilisation and their determinations – Malnutrition and its problems – Nutrient supplementation – fortification - Nutritional labeling and its importance - Effect of processing on protein quality, essential amino acids - Digestibility, carbohydrates in food and dietary fibre Unit IV Inborn errors of carbohydrate, protein and fat metabolisms - Nutrition and disorders associated with organs such as liver and kidney - Naturally occurring anti-nutritional factors – Cyanogens, lectins, enzyme inhibitors, phytoallexins, phytates Unit V Nutrition for specialized purposes – Paediatric nutrition – geriatric nutrition – Sports nutrition – Nutrition during pregnancy - Functional foods Ageing –Theories of ageing – Nutrition and ageing – Cancer and its prevention - Age-related metabolic disorders – Nutrition in the treatment of age-related disorders like hypertension, diabetes, Alzheimer’s disease

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Text / Reference Books: 1. Lehninger A.L, Nelson D.L., M.M. Cox, Principles of Biochemistry, CBS Publications, 1993. 2. Voet D, Voet G, Biochemistry, Second Edition, John Wiley and Sons, 1994. 3. Stryer L, Biochemistry, Fouth edition, 1994. 4. Food and Nutrition, Wahlquist M L, 1997 5. Principles of Human Nutrition – Second Edition, Edited by Martin Eastwood, Blackwell Publishing 6. Functional foods and Nutraceuticals in Cancer Prevention, Edited by Ronald Ross Watson

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School of Biotechnology

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BC205 FOOD BIOCHEMISTRY LAB Credit : 0:0:2 Marks (50+50) 12 experiments will be notified by the HOD from time to time

BC206 INSTRUMENTAL METHODS OF ANALYSIS Credit : 4:0:0

UNIT I Buffer Solutions Definitions, preparations, derivation of Henderson-Hasselbalch equation and its application, buffering systems of blood, determination of pH using H 2 electrode and glass electrode.

UNIT II Colorimeter, Flourimeter, Flame Photometer and Spectrophotometer Beer - Lambert’s law, Principle, description and application of Colorimeter, Flourimeter, Flame photometer and Spectrophotometer. UNIT III Chromatography and Electrophoresis Chromatography- principles, types - paper, thin layer, adsorption, ion-exchange, affinity, gel filtration, gas and HPLC. Electrophoresis – principles, types – disc, Isoelectric focussing, immuno-electrophoresis, isotachophoresis, supporting materials-paper, starch, agarose, polyacrylamide. UNIT IV Radio active techniques Radioactive isotopes, radioactive decay and their types, radioactive techniques-RIA, GM counter, Scintillation counter, Autoradiography, Applications in Medicine & Diagnosis UNIT V Thermo Analytical Techniques Theory of thermal analysis- thermo gravimetric- Basic theory, construction and working of Differential Thermal Analysis (DTA) and Differential Scanning Calorimeter (DSC) Text Book 1. Willard and Merrit, Instrumental Methods and Analysis. VI Edition, CBS Publishers & Distributors. Reference Books: 1. Ewing GW, Instrumental methods of Chemical Analysis, McGraw Hill Book Company, 1989. 2. Braun. H, Introduction to Chemical Analysis, McGraw Hill, 1987.

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BC207 INSTRUMENTAL METHODS OF ANALYSIS LAB Credit : 0:0:2 Marks (50+50) 12 experiments will be notified by the HOD from time to time BC208 BIOPHYSICS Credit : 4:0:0

Marks (40+60)

UNIT I Introduction Levels of structures in Biological macromolecules. Central questions in biophysics, basic strategies in biophysics.

UNIT II Conformational Analysis Forces that determine protein and nucleic acid structure, basic problems, polypeptide chains geometrics, potential energy calculations, observed values for rotation angles, hydrogen bonding, hydrophobic interactions and water structures ionic interactions, disulphide bonds. UNIT III Structural Analysis of Macromolecules Prediction of proteins structure, nucleic acids, general characteristics of nucleic acid structure, geometrics, glycosidic bond rotational isomers and those puckering backbone rotational isomers and ribose puckering forces stabilising ordered forms, base pairing, base stacking tertiary structure of nucleic acids. UNIT IV Kinetics of Ligand Interaction Biochemical Kinetics studies, unimolecular reactions, simple biomolecular multiple intermediates, steady state kinetics, catalytic efficiency, relaxation spectrometry, ribonuclease as an example. UNIT V Techniques for the Study of Biological Structure & Function Size and shape of macromolecules, methods of direct visualisation, macromolecules as hydrodynamic particles, macromolecular diffusion, ultracentrifugation, viscometry X-ray crystallography – X–ray diffraction, determination of molecular structures ,electron microscopy, neutron scattering, light scattering.

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Text Book 1. Cantor R., Schimmel P.R., Biophysical Chemistry, Vol. I, II, W.H. Freeman & Co., 1985. Reference Book 1. Daniel. M, Basic Biophysics for Biologists, 1998.

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Unit II. Principle and working methodology of compound microscope-structure of prokaryotic and eukaryotic cell. classification and reproduction mode of bacteria – their beneficial and harmful effects.Anatomy of animals Digestive system – Structure of tongue.SUBJECTS (Microbiology) Code MB101 MB102 MB201 MB202 MB203 MB204 MB205 MB206 MB207 MB208 Subject Name Introduction to Biology Introduction to Biology Lab Cell Biology Cell Biology Lab Microbiology Microbiology lab Molecular Biology Molecular Biology lab Food Microbiology Food microbiology lab Credit 3:0:0 0:0:1 4:0:0 0:0:1 4:0:0 0:0:1 4:0:0 0:0:1 4:0:0 0:0:2 MB101 INTRODUCTION TO BIOLOGY Credit : 3:0:0 Unit – 1: Biodiversity Biodiversity in living organisms–systems of classification–taxonomic systems-nomenclature methods. Unit III . chemosynthesis. a K n u r a y i n U s r e v y it Marks (40+60) School of Biotechnology 6 . types and structure of both plant and animal viruses– diseases caused by them– life cycle of phage and HIV. small intestines and Liver Respiratory system – structure of nasal cavity. trachea and lungs Circulatory system – Components of circulatory system – structure of blood vessels – Types of blood vessels – Structure of heart Urinary system – Structure of kidneys and nephron Reproductive system – Brief description of the male and female reproductive organs Unit – IV : Physiology of plant Cell as a physiological unit-absorption. Salient features of fungi and algae. Reproduction in angiosperms-Germination of seeds. dicot leaf. stomach. Stems: monocot –dicot. pharynx. Translocation of minerals and solutes. pharynx. oesophagus.C4 and C 3 pathway– Photorespiration (C 2 cycle). teeth. transport and transpiration of water.Anatomy of plants Tissue and tissue systems – anatomy of Roots: monocot –dicot.cell division. mode of nutrition. Plant growth. Photosynthesis : photophosphorylation.

lysosomal and vacuolar membrane ATP dependent proton pumps. serine – threonine kinases in signalling. blood cells. First edition. quantitation and characterisation of receptors. phosphorylation and regulation of protein kinases in signalling. UNIT – III Receptors and Models of Extra Cellular Signalling Cytosolic. H. First edition. mechanism of breathing.Micelles. Excretion – mechanism of urine formation Chemical co-ordination systems Text Books: 1. Regulation of respiration. autocrine.Unit V. endocytosis and exocytosis. Respiration – Process. Circulation – Functioning of human heart. cytoskeletal proteins. calcium ion flux and its role in cell signaling. paracrine and endocrine models of action. cell cycle. nuclear and membrane bound receptors. biosynthesis of inositol tri phosphates and their role as messengers. cyclic GMP and G proteins in signal transduction. 2004 & 2005. gaseous exchange in alveoli. Absorption and assimilation. contractile proteins – actin & myosin. trans-membrane potential coupled ATP generation.First and Second year Tamilnadu Text Book Coporation.C. Tamilnadu Text Book Coporation. role of cyclic AMP. Cell division: mitosis & meiosis. permeases. Ca2+ ATPase pump. co transport. 2. UNIT – II Transport Across Cell Membranes Passive & active transport. Cardiac cycle. MB102 INTRODUCTION TO BIOLOGY LAB Credit : 0:0:1 6 experiments will be notified by the HOD from time to time MB201 CELL BIOLOGY Credit : 4:0:0 UNIT – I Structure and Function of the Cell and Its Organelles Eukaryotic and prokaryotic cells: principles of membrane organization . Origin and conduction of heart beat. Entry of virus and toxins into cells.Animal and Human Physiology Digestion – process. molecules that control cell cycle. extra cellular matrix. examples of receptors. a K n u r a y i n U s r e v y it Marks (50+50) Marks (40+60) School of Biotechnology 7 . symport. antiport. different models of signal amplifications. sodium potassium pump. Blood – composition. UNIT – IV Signal Transduction Signal amplification. Transport into prokaryotic cells. Higher Secondary First and Second year. 2004 & 2005.S. ion-selective gated channel against neuronal cell membrane. Biology (Zoology). membrane proteins. Biology (Botany).

Kimball T.. algae and fungi with a special mention of life history of actinomycetes. 2nd edition. aerobic and anaerobic bioenergetics. Inc. James D. differentiation. Culture of Animal Cells. De Robertis & De Robertis. primary cultures. Molecular Biology of the Cell. classification and nomenclature of microorganism. morphological analysis. Cell Biology. Text Books: 1.. host-microbe interactions. Ian Freshney R. Wesley Publishers. mycoplasma and bacteriophage. microscopic examination of microorganisms. W. UNIT – II Microbes-Structure and Multiplication Structural organization and multiplication of bacteria. role of matrix in cell growth. Cell line: generation of cell lines. Liss.H. techniques in cell culture. Baltimore D. history of microbiology. 3. characterization of cells. a K UNIT – III Microbial Nutrition.Watson. References: 1. acid fast. Darnell J. anti-fungal and anti-viral agents. Growth And Metabolism Nutritional requirements of bacteria and different media used for bacterial culture. Lodish H.UNIT – V Cell Culture Techniques for the propagation of eukaryotic and prokaryotic cells.. 2. clinically important microorganisms. viruses. 2. UNIT – IV Control of Microorganisms Physical and chemical control of microorganisms. utilization of energy for biosynthesis of important molecules.Freeman. explant cultures. New York. MB202 CELL BIOLOGY LAB Credit : 0:0:1 6 experiments will be notified by the HOD from time to time MB203 MICROBIOLOGY Credit : 4:0:0 UNIT – I Introduction Basic of microbial existence. three dimensional cultures. maintenance of stock cells. Cell Biology. yeast. flagellar staining.W. principles of different staining techniques like gram staining. anti-bacterial. immunochemistry. Alan R. capsular staining. growth curve and different methods to quantitate bacterial growth. contamination. light and electron microscopy. n u r a y i n U s r e v y it Marks (50+50) Marks (40+60) School of Biotechnology 8 . Molecular Cell Biology. mode of action and resistance to antibiotics.

C. biosensors. DNA repair. secondary metabolites and their applications. UNIT – II DNA Replication Replication in prokaryotes and eukaryotes. microorganisms and pollution control.N. biogas. post-translational modifications. Kathmandu. Casita. Prasad B. Klein DA. Oxford & IBH.P. Microbiology.RNA splicing. School of Biotechnology n u r a y i n U s r e v y it Marks (50+50) Marks (40+60) 9 . process of translation in prokaryotes and eukaryotes. India. “Biotechnology in Sustainable Biodiversity and Food Security” (2003). UNIT – IV Translation Elucidation of genetic code. 1996.. a K UNIT – III Transcription Prokaryotic and eukaryotic transcription. RNA editing. Organization of eukaryotic chromosome – cot value. bioremediation. Box 20195. inhibitors. suppressor mutation. New Delhi. 2. Microbiology. MB204 MICROBIOLOGY LAB Credit : 0:0:1 6 experiments will be notified by the HOD from time to time MB205 MOLECULAR BIOLOGY Credit : 4:0:0 UNIT – I Classical Genetics Mendelian genetics. Foundations in Microbiology. replication of linear viral DNA. Nepal. crossing over.B12.. Prescott LM.Lac and trp operons. Brown Publishers.. Tata McGraw Hill Edition. Pelczar and Reid. 1993. Chan ECS And Krein NR. mycorrhizae (AMFungi) and biopesticides. ribozyme. transcription factors. Pelczar MJ. 3. leaching of ores by microorganisms. transduction and transformation. 3 rd Edition.UNIT – V Industrial and Environmental Microbiology Primary metabolites. production of penicillin. C. Talaron K. Avery McLeod & McCarty.Brown Publishers.N. Prasad B. alcohol. Text Book 1. G. Talaron A. Bacterial conjugation. Wm. cyanobacteria. post-transcriptional modification . (2003) Budha Academic Enterprises. replication of telomeres in eukaryotes. Harley JP. Vit. features of promoters and enhancers. W. D-loop and rolling circle mode of replication. biofertilizers rhizobia. 2. References 1. “A Text Book of Biotechnology”. UNIT – V Regulation of Gene Expression Regulation at various stages of gene expression in eukaryotes and prokaryotes . preservation of food. inhibitors of protein synthesis. New Delhi.O. linkage. classical experiments – Hershey and chase.

Endospore formers Irregular non-sporing gram positive rods their role in food spoilage Molds. Yeast. Molecular Biology. Moisture and water activity. sampling. Gene VII. mold and Yeast – nutrition. electrical methods. 1999 2. Principles of Genetics. water.Factors affecting growth of bacteria. House.. Molds in the category of Zygomycetes. Hopkins WH. Narosa Publ. Gardner / Simmons / Snustad. Benjamin Lewin. 1987 MB206 MOLECULAR BIOLOGY LAB Credit : 0:0:1 6 experiments will be notified by the HOD from time to time MB207 FOOD MICROBIOLOGY Credit : 4:0:0 Unit I Micro organisms associated with foods: Bacteria – Gram negative aerobic Rods. Natural food preservatives Brief overview of Modified storage packaging a K n u r a y i n U s r e v Marks (50+50) y it Marks (40+60) School of Biotechnology 10 . Estimating number of microorganisms. Definitions. antimicrobial barriers and constituents Unit II Sources of microorganisms – Soil. Deuteromycetes etc. total cell counts and viable cell counts. Molecular Biology of the Gene. temperature. Weiner AM. Roberts JW. Useful microorganisms – Lactic Acid Bacteria. Redox potential. Plants and of animal origin.Fungi as Food. Alternative methods – Dye-reduction tests. Oxford University Press. Carbon and Nitrogen Sources. Gram negative facultative anaerobic Rods.: Viruses. aseptic collection of samples. John Wiley Reference books 1. Eighth Edition. Bacterial viruses and Fungal Viruses . Watson JD. mold and fungi. Definitions . UV and ionizing radiation High pressure processing – Pascalisation Low-temperature storage – Chilling and freezing Use of chemical preservatives. Steitz JA.Gram Negative anaerobic rods . 2000 2. David Friefelder. pH conditions.Text Books 1. sample size. Intestinal viruses. plate counters. importance of molds. ATP determination – Rapid methods for the detection of specific organisms and toxins – Immunological methods and DNA/RNA methodology Microbiological quality control and HACCP Unit III Heat processing – Pasteurisation and appertization – determination of D and z values – heat sensitivity of micro-organisms – spoilage of canned foods – aseptic packaging Irradiation – Brief account of microwave.

Gustavo V Barbosa-Canovas Food Science and Food Biotechnology: CRC Press 2003 2. (Third Edition) CRC Press December 2003 MB208 FOOD MICROBIOLOGY LAB Credit : 0:0:2 12 experiments will be notified by the HOD from time to time a K n u r a y i n U s r e v y it Marks (50+50) School of Biotechnology 11 . Fruits and Fruit products.O. W. Spongiform encephaolpathies Text Books 1. Staphylococcus aureus. New York (1968) 2. Salmonella. Brucella. Food Microbiology. Gustavo F Gutierrez-Lopez. Hepatitis A and B. Second Edition. Adams and M. Gastroenteritis viruses.Frazier: Food Microbiology (II edition or later) Mcgraw Hill Book Company. Escherichia coli. Camphylobacter. Scombrotic fish poisoning. Panima Publishing corporation. Moss. Third reprint 2004 References: 1. Bacillus cereus. Clostridium botulinum. Bibek Ray: Fundamental Food Microbiology. M.C.R. Nuts and Oilseeds. New Delhi. Pulses.Unit IV Microbiology of Food commodities Cereals. Vegetables and Vegetable products Unit V Food borne pathogens Aeromonas hydrophila.

effect of temperature and pressure on equilibrium constant. UNIT – V Biochemical Thermodynamics Energetics of metabolic pathways energy coupling (ATP and NADH).SUBJECTS (Chemical Engineering) Code CL201 CL202 CL203 CL204 CL205 CL206 CL207 CL208 CL209 CL210 Subject Name Chemical Thermodynamics & Bio-thermodynamics Principles of Chemical Engineering Chemical Engineering Lab Unit Operations Chemical Reaction Engineering Mass Transfer Operations Process Engineering Calculations Unit Operations in Food Processing . v-l-e calculations for binary and multi component systems. calculation of equilibrium conversion and yields for single and multiple reactions. n u r a y i n U s r e v Marks (40+60) y it School of Biotechnology 12 . liquid-liquid equilibria and solid-solid equilibria. UNIT – III Phase and Chemical Reaction Equilibria Criteria for phase equilibria. calculations involving actual property exchanges. concepts of chemical potential and fugacity. calculation of real irreversible processes. residual properties. yield coefficients. concepts and applications of excess properties of mixtures. liquefaction. Maxwell’s relations and applications. evaluation of equilibrium constant. entropy generation. UNIT – II Solution Thermodynamics Partial molar properties. power cycle. ideal and non-ideal solutions. a K UNIT – IV Thermodynamic Analysis of Processes Concept of lost work.I Heat Transfer and Fluid Flow Unit operations in Food Processing – II Credit 4:0:0 4:0:0 0:0:2 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 CL201 CHEMICAL THERMODYNAMICS AND BIOTHERMODYNAMICS Credit : 4:0:0 UNIT – I Thermodynamic Properties of Fluids Volumetric properties of fluids exhibiting non ideal behavior. composition models. Equilibrium criteria for homogeneous chemical reactions. degree of reduction concepts-available –electron balance. oxygen consumption and heat evolution in aerobic cultures. Gibbs Duhem equation. activity coefficient. thermodynamics efficiency of growth. estimation of thermodynamic properties using equations of state. stochiometric and energetic analysis of cell growth and product formation-elemental balances.

Elseviers. JA. recycle and by pass.M. steady and unsteady state calculations. Royels. 5th Edition. Chemical And Engineering Thermodynamics.. 2.V.3 Edition. characteristics. UNIT – V Flow Through Packed Columns Fluidisation. material balances without and with chemical reactions. unit operations.C. 3.. humidity calculations. Bhatt B. environment.1989. McCabe W. integration. Prentice Hall India. Unit Operations In Chemical Engineering. compressors. 1983 CL202 PRINCIPLES OF CHEMICAL ENGINEERING Credit : 4:0:0 UNIT – I Overview of Process Engineering Mass and energy conservation. Stoichiometry.L. Sandler S. degrees of freedom. Narayanan K. Abbot M. compressible fluid flow concepts. numerical differentiation. sensible heat. 2001. work a K Text Books rd 1. Chemical Engineering Thermodynamics. A Text Book Of Chemical Engineering Thermodynamics. Geankoplis C. applied mathematics for experimental curve fitting..M.C. McGraw-Hill. 2002. 1977.J. UNIT – IV Fluid Mechanics Fluids. McGraw-Hill Inc. Smith J. latent heat. UNIT – III First and Second Laws of Thermodynamics Energy balances. 2.I. Prentice Hall India. Harriot P..Text Books 1.M. Reference books 1.I. multiphase flow concepts. 1993. turbulent pressure drops. laminar. John Wiley. Transport Processes And Unit Operations. SI units. steady and unsteady state. Tata McGraw-Hill. fluid flow. Reference books 1. UNIT – II Material Balances Overall and component balances. Van Ness H. conversion factors. vapour pressure. 6th Edition. centrifugal and piston pumps. process automation. Vora S. n u r a y i n U s r e v Marks (40+60) y it School of Biotechnology 13 . 2001. Kinetics and Energetics in Biotechnology. Smith J. fluid statics and applications in chemical engineering..

Prentice Hall India. Unit Operations In Chemical Engineering. constant volume batch filtration. McCabe W. combined resistances. Smith J. lumped heat capacity. unsteady state conduction. gas-solid suspensions. Text books th 1. agitation of liquids. Reference books 1. Transport Processes And Unit Operations. industrial filters. concentration and temperature dependence. forced and natural convection.IV Heat Transfer – Convection and Radiation Dimensional analysis. Fundamentals Of Heat And Mass Transfer.. 5 Edition. Incropera F. School of Biotechnology 14 .C. continuous filtration. mass and enthalpy balances.Credit : 0:0:2 CL203 CHEMICAL ENGINEERING LAB Marks (50+50) 12 experiments will be notified by the HOD from time to time CL204 UNIT OPERATIONS Credit : 4:0:0 Marks (40+60) UNIT – I Mixing and Agitation Dimensional analysis.P. evaporators.2002. settling and sedimentation.J. Mcgrawhill. CL205 CHEMICAL REACTION ENGINEERING Marks (40+60) a K Credit : 4:0:0 n u r a y i n U s r e v y it UNIT – I Scope of Chemical Kinetics & Chemical Reaction Engineering Broad outline of chemical reactors. UNIT – II Filtration Constant pressure. Heat transfer to non-newtonian fluids Radiation – Basic concepts UNIT – V Heat Exchangers Equipments. convection in flow over surfaces through pipes boiling and condensation. development of rate equations for different homogeneous reactions. 2. single and multiple effects.1998. UNIT . Industrial scale reactors. NTU concept. centrifugation. extended surfaces. rate equations. power for agitation. design of heat exchangers. combined conduction and convection.1993. gas-liquid systems. Geankoplis C. overall heat transfer coefficients.Conduction Steady state conduction. UNIT – III Heat Transfer . agitator scale up.L. John Wiley.

Mims C. Leaching Principles.1999. Fogler H. Continuous distillation. Gas-Liquid Reactions Resistances and rate equations. John Wiley. reactions steps.A.. Levenspiel O. semi-batch reactors.1999. Analogies in Transport Phenomenon.S.. UNIT – V Fixed Bed and Fluid Bed Reactors G/l reactions on solid catalysis.A. multiple reactions. Chemical Reaction Engineering. NTU concepts. Industrial distillation equipments. HTU and NTU concepts. 2. UNIT – IV Gas-Solid. performance equations for single reactors. Steam and Flash Distillation.Unit – II Ideal Reactors Isothermal batch. Prentice Hall India. Introduction To Chemical Reaction Engineering And Kinetics. three phase-fluidized beds. Simple. Staged and continuous extraction. tank reactors. Solid-liquid equilibria. trickle bed. HETP. Reference Books 1.W. a K UNIT – III Vapour Liquid Operations V-L Equilibria. flow. UNIT – II Gas Liquid Operations Principles of gas absorption. multiple reactor systems. Saville B. non-ideal flow models. reactors for fluid-fluid reactions. McCABETHIELE & PONCHON-SAVARIT Principles. Missen R. UNIT – IV Extraction Operations L-L equilibria. UNIT – III Ideal Flow and Non Ideal Flow RTD in non-ideal flow.2002 CL206 MASS TRANSFER OPERATIONS Marks (40+60) Credit : 4:0:0 UNIT – I Diffusion and Mass Transfer Molecular diffusion in fluids and solids. resistances and rate equations. Industrial absorbers. slurry reactors. Mass Transfer coefficients. Text Books rd 1. John Wiley. Absorption with Chemical Reaction. Design principles of absorbers. Interphase Mass Transfer. n u r a y i n U s r e v y it School of Biotechnology 15 . heterogeneous catalysis. reactor performance with non-ideal flow. 3 Edition. Elements Of Chemical Reaction Engineering. HTU. Single and Multi component absorption.

Mcgraw Hill. Stanley E Charm: The fundamentals of Food engineering. Text Books 1. Drying-Mechanism-Drying curvesTime of Drying. conventions in methods of analysis and measurement. Prentice Hall of India. (7th Edition. 3 edition. vapour equilibria for multi-component systems. chemical equations and stoichiometry Unit II: Gases.Toledo: Fundamentals of Food Process Engineering. general balance with or without reactions. Unit III : Material Balances Material balance of food processes involving Dilution..J. Geankoplis C. Principles and selected applications: Academic Press. material balances involving condensation and vaporization. temperature. Merson: Food Engineering.UNIT – V Solid Fluid Operations Adsorption equilibria – Batch and fixed bed adsorption. enzymatic reactions. 3rd edition. AVI Publishing company. B. Unit V : Kinetics of Chemical Reactions in Foods Theory of reaction rates. Coulson and Richardson’s Chemical Engineering. calculations of enthalpy changes. Mole unit. Loncin and R. Asian Books Pvt Ltd. Romeo T. New York (1991) 2. review of SI system of measurement. and reaction rate constant. 1981. Concentration and Dehydration. CL207 PROCESS ENGINEERING CALCULATIONS Marks (40+60) Credit : 4:0:0 Unit I : Introduction to Food Engineering Calculations: systems of measurements. Liquids and Solids Ideal Gas relationships. Reference books 1.M. Vapours. types of reactions. Units and Dimensions. pressure. London 4. basis. 2004) 3. L. Multistage processes and numerical examples Unit IV : Energy Balances Concept and units. Treybal R. Himmelblau D.E. heats of solution and mixing: Unsteady state material and energy balances. & J. New York (1981) n u r a y i n U s r e v y it School of Biotechnology 16 . Batch and continuous dryers. Van Nostrand Reinhold. temperature dependence of reaction rates a K Text/Reference Books 1. Transport Processes and Unit Operations. rd 2. 1998. Mass Transfer Operations. M.Riggs: Basic Principles and Calculations in Chemical Engineering : Prentice Hall International. Vol I & II. 2002.

Marcel Dekker Inc. Richardson. Butterworth & Heinemann . plate evaporators. Fifth Edition. John Wiely & sons Publishers. Volatility etc. Marcus Karel & Deryl B Lund: Physical Principles of Food Processing.M. Viscosity. Vapour recompression.Earle: Unit Operations in Food Processing. 2. Steam distillation.Coulson & Richardson's Chemical Engineering . H.A. Pergamen Press. Mass transfer operations. extraction of Fatty acids. Heat economy. Mass transfer phenomenon applied to food systems Unit III : Evaporation Needs. theory and examples. Coulson. USA 3. limitations and economics Text/Reference Books 1. D. Solubility. Applications. Boston. Calculations. Basics of Solid-liquid extraction processes. Relative advantages. dimensionless ratios. Thermo and mechanical systems.Lenigar and W. (II Edition or later). H. J. J. Molecular distillation.L. Albert Ebarz: Unit Operations in food Processing.Beverlo: Food Process Engineering. boiling point elevation. SCFE systems and components. Single and multiple effect evaporation. and calculations involving various physical parameters. Solid liquid equilibria. Equilibrium concentration relationships. Hops. Falling film. Properties of Super critical CO2 . Units and dimensions of engineering parameters.Elsevier science Ltd . 6. operating conditions. J. Essential oils and Bio chemicals.F. Crystallization Unit V : Super Critical Fluid Extraction Super critical Fluid State.Vol 2 Particle Technology and Separation Processes. Supercritical Phase Equilibria. Density. New York CL208 UNIT OPERATIONS IN FOOD PROCESSING I Credit : 4:0:0 Marks (40+60) Unit I : Introduction Basic principles of food processing.Reidal Publishing Co. Unit IV : Contact Equilibrium Separation Processes Concentrations. Ramaswamy & M. Gas-liquid equilibria. Conservation of mass and energy. applications and equipment. Clair Batty and Steven L Folkman: Food Engineering Fundamentals. Unit II : Distillation Distillation. Markotte: Food Processing Principles and Applications. R.A. liquid-liquid extraction extraction.5. with food application examples. CRC Press Ltd. Harker . basic principles. a K n u r a y i n U s r e v y it School of Biotechnology 17 .H. thin film and scraped surface evaporators. 2002. J. climbing film tubular evaporators.S. New York 4. CRC Press 5.

Peclet number. pressure dynamics. Heldman and R. sphere Steady heat conduction in simple geometries – Plane wall. natural convection and unsteady heat conduction and the significance of these numbers – Reynold’s number. USA (2003) CL209 HEAT TRANSFER AND FLUID FLOW Credit : 4:0:0 Marks (40+60) Unit I : Fluid flow Theory Applications Fluid statics. Zacharias B.boundary layer theory. New Delhi.theorem Text books 1. critical radius of insulation Overall heat transfer coefficient Basics of Radiation heat transfer Unit IV : Convective Heat Transfer (Forced Convection) Convection heat transfer . Rotameter. Tata McGraw Hill Publishing Company Ltd. Forced convection inside tubes and ducts Heat transfer coefficient for Laminar flow in a tube with constant heat flux and constant wall temperature a K Unit V : Dimensional Analysis and Dimensionless Parameters Principle of similarity applied to heat transfer Derivation of dimensionless parameters from the differential equations for forced convection. Venturimeter. hot wire anemometer Unit III : Conductive Heat Transfer Conduction. Dennis R.7. 2002 n u r a y i n U s r e v y it School of Biotechnology 18 . Maroulis and G. the Nozzle.. thermal boundary layer. D. Cylindrical wall with and without heat generation Transient heat conduction – Lumped capacitance method for bodies of infinite thermal conductivity Theory of insulation. Nag. Newtonian and non Newtonian fluids. Bernoullie’s equation. Turbulent flow over a flat plate. Simple calculations.K. Grashof number and Fourier number Dimensional analysis by Rayleigh’s method and Buckingham pi. (1981) 8. Paul singh: Food Process Engineering (II Edition). Drag coefficient and drag force. Flow over a flat plate. Laminar and Turbulent flow. Heat Transfer by P. Unit II : Flow Measuring Devices Orificemeter. AVI Publishing Company Inc. Boundary layer thickness and Skin friction coefficient. Equations for heat conduction through a cylinder. the Notch or weir. Evaluation of convection heat transfer coefficient. Saravacos: Food Process Design Marcel Dekker. Pitot tube. Nusselt number. friction losses in pipes valves and bends.

Applications in food industry. Pumps and other membrane equipment. Tubular Bowl. Unit V : Roasting and Cooking Theory. Unit IV : Membrane Filtration Definitions. G. Steam Cookers. Sixth Edition. Different types of centrifuges – Basket. Marcel Dekker Inc. Diffusion of aroma components Applications of diffusion and migration in food packaging. Diffusion in porous foods. Coulson.M.Schneider. Membranes and their characteristics. Chennai 5.Richardson: Chemical Engineering VoI 1. relative advantages and limitations.Vol 2. Disk bowl. Molecular weight cut off in each case. Diffusion of gases in liquid and solid foods. an imprint of Elservier. Advantages of Extrusion cooking over conventional cooking.J. Rakesh Singh and S.H. Nano filtration (NF). Rielly: Chemical engineering for the Food Industr. feed and discharge arrangement in each case.D. J. Nozzle centrifuges. E. 2006. Different kinds of Roasters. Forming extruders and snack food extruders. Fifth Edition. Brennen: Food Engineering Operations (Vol 1 and 2).Coulson and J. Blackie Academic and Professional. Text/Reference Books 1.Coulson & Richardson's Chemical Engineering. Richardson. London. Particle Technology and Separation Processes. D.L.H. Ultra filtration (UF) and Micro filtration (MF). Moisture transfer in foods. Unit II : Mechanical Separations Filtration and sedimentation. Single and Twin Screw Extruders. Reverse Osmosis (RO). J. Desludging (Self Cleaning) Bowl Centrifuges. membrane materials.F. Indian Reprint.2. F. Butterworth & Heinemann . Verlag Chemie publishing. Cross flow filtration.M. CL210 UNIT OPERATIONS IN FOOD PROCESSING II Credit : 4:0:0 Marks (40+60) Unit I : Adsorption and Diffusion Basics of adsorption. Extrusion cooking.M. 2. Sedimentation of solid particles in gas media. Pyle and C. Fryer. advantages and applications. Butterworth-Heinemann.F. Decanting.Wilke: Extraction with Supercritical Gases. Harker . J. gravity sedimentation. Basel 4. Elsevier Publishing a K n u r a y i n U s r e v y it School of Biotechnology 19 . Specific characteristics.Elsevier science Ltd . Heat Transfer and Mass Transfer. Configuration of membranes. Hong Kong 3. Basic equations. 2002. Examples Industrial applications and equipments for Filtration and Sedimentation Unit III : Centrifugal Separation Sedimentation and filtration centrifuges. J. Importance of balancing of rotating masses. Inter-phase moisture transport. Stahl and G. Fluid flow.Rizwi: Bio Separation Process in Foods.

Lewis: Separation processes in the Food and Biotechnology industries (Principles and applications): Woodhead publishing Ltd. Jimmy L. A. USA 1986 a K n u r a y i n U s r e v y it School of Biotechnology 20 . Humphry and G. Maynord Joslyn and J.L. Mcgraw Hill New York 7. Keller: Separation Process Technology.Grandison and M.J. E. Cambridge UK 8.Heid: Food Processing Operations (3 Volumes) AVI Publishing company Inc.S.6.

Printer on information theory. http. NCBI. EMBL. DDBJ – protein databanks – sequence databanks – PIR. GCG Sequence Analysis(Basic concepts only) UNIT IV : Evolutionary Trees and Phylogeny Ultrasonic trees – parsimony – Ultrametric problem – Perfect phylogeny – Phylogenetic alignment – connection between multiple alignment and tree construction. TrEMBL _ structural databases – PDB. 1997. Text Book 1. Databanks – nucleotide databanks – Genbank. UNIT V : Special Topics in Bioinformatics DNA Mapping and sequencing – Map alignment – Large scale sequencing and alignment – Shotgun – DNA sequencing – Sequence assembly – Gene predictions – Molecular predictions with DNA strings. Unit III : Sequence Databases and Their Uses Introduction to databases – database search – Algorithms issues in database search – sequence database search – FASTA – BLAST – Amino acid substitution matrices PAM and BLOSUM. a K n u r a y i n U s r e v y it Marks (40+60) School of Biotechnology 21 . SWISSPROT. CATH. Cambridge University Press. SCOP. ftp.SUBJECTS (Bioinformatics) Code BI201 BI202 BI203 BI204 BI205 BI206 BI207 BI208 BI209 Subject Name Basics of Bioinformatics Genomics Proteomics Bioinformatics Lab I Molecular Evolution and Phylogeny Molecular Modeling and Structure Prediction Biopharmaceutical Technology Bioinformatics Lab II Introduction to System Biology Credit 4:0:0 4:0:0 4:0:0 0:0:2 4:0:0 4:0:0 4:0:0 0:0:2 4:0:0 BI201 BASICS OF BIOINFORMATICS Credit : 4:0:0 Unit I Introduction to Bioinformatics Scope of Bioinformatics – Elementary commands and Protocols.Dan Gusfield. telnet. UNIT II : Sequencing Alignment and Dynamic Programming Introduction – Strings – Edit distance two strings – string similarity local alignment -gaps – Parametric sequence alignments – suboptimal alignments – multiple alignment – common multiple alignment methods. Algorithms On Strings Trees And Sequences.

Functional and Computational Aspects. 3. Pennington. Introduction to Bioinformatics 2.M.Sequence complexity – Introns and Exons – Genome structure in viruses and prokaryotes – Isolation of chromosomes – chromosome microdissection – Retrofitting UNIT II Sequencing methods and strategies – Automated DNA sequencing – DNA sequencing by capillary array electrophoresis – High throughput sequencing – Restriction enzyme fingerprinting – marker sequences – Hybridization assays – Physical mapping without cloning. 1999. Proteomics and Bioinformatics n u r a y i n U s r e v Marks (40+60) y it School of Biotechnology 22 . Bioinformatics. Proteomics and Bioinformatics 4. John Wiley & Sons BI202 GENOMICS Credit : 4:0:0 UNIT I Introduction – Organization and structure of genomes – Genome size . UNIT III Genome annotation – traditional routes of gene identification – detecting open-reading frames – software programs for finding genes .Reference Books 1.B. S. S. a K Reference books: 1. UNIT V Analysis of Genomes – Human – Mouse – Plasmodium Falsiparum – Saccharomyces cerevisiae – Mycobacterium tuberculosis. Baxevenis. 2. Discovering Genomics. Proteomics from Protein Sequence to Function 3. Campell. Twyman Principles of Genome analysis and Genomics –– Blackwell Publishing. Genomics and Proteomics. Discovering Genomics. Campell. Plenum Publication. Sahai.Primrose and R. Lesk.Identifying the function of a new gene – gene ontology – overview of comparative genomics – Protein structural genomics – introduction – determining gene function by sequence comparison and through conserved protein structure UNIT IV Global expression profiling – Introduction – traditional approaches to expression profiling – analysis of RNA expression – applications of genome analysis and genomics.

De novo sequencing using mass spectrometric data. Discovering Genomics. Pharmaceutical Applications – Proteomics in drug Discovery in human .Two-dimensional polyacrylamide gel electrophoresis.Archaeology of the genome.Solubilization.Reproducibility of 2-DE.Correlative mass spectrometric based identification strategies.Detecting proteins in polyacrylamide gels-Image analysis of 2-DE gels UNIT III Mass spectrometry based methods for protein identification.drug development and toxicology.fundamentals of Population genetics-The nature of molecular evolution. I edition.Proteomics and the new biology UNIT II Analysis of proteomes. Resolution.phage antibodies as toolsGlycobiology and Proteomics.BI203 PROTEOMICS Credit : 4:0:0 Marks (40+60) UNIT I Introduction to Proteomics – The Proteome – Mining proteomes.Sample preparation.Types of micorarrays-Designing a microarray experimentMicroarray Technology in Treating Disease UNIT V Application of proteome analysis.Bridging Genomics and Proteomics.2-DE gel electrophoresis coupled with mass spectrometry UNIT IV Micro array techniques. Reference book 2. Proteomics and Bioinformatics Credit : 0:0:2 a K n u r BI204 BIOINFORMATICS LAB .In plant genetics and breeding Text book 1. Campell. Pennington & Dunn.I Marks (50+50) a y i n U s r e v y it 12 experiments will be notified by the HOD from time to time BI205 MOLECULAR EVOLUTION AND PHYLOGENY Marks (40+60) Credit : 4:0:0 UNIT I Introduction to molecular evolution. Reduction.Driving forces in evolutionevolutionary changes in nucleotide sequences School of Biotechnology 23 . Proteomics from protein sequence to function.

Holmes Molecular evolution. Dan Graur and Wen-Hsiung Li.Genetic variation within speciesNatural selection UNIT V Applications of molecular phylogenetics.Distance matrix methods. Edward C. Hydrogen Bonding in Molecular Mechanics. (October 1998) BI206 MOLECULAR MODELLING AND STRUCTURE PREDICTION Credit : 4:0:0 a K UNIT Introduction . Maximum likelihood methods.UNIT II Molecular phylogenetics.Useful Concepts in Molecular Modelling : Coordinate Systems.Sinauer Associates.analysis of true tree.Problems associated with phylogenetic reconstruction UNIT IV Models of molecular evolution. Electrostatic Interactions.Organismal phylogeny.Gene trees and species trees.speciation Reference books: 1. Van der Waals Interactions..Molecular phylogenetic examples. Mathematical Concepts. INC. Molecular Graphics. A phylogenetic approach. II ed. n u r a y i n U s r e v y it Marks (40+60) School of Biotechnology 24 . Potential Energy Surfaces.Energy Minimisation and Related Methods for Exploring the Energy Surface.. The Molecular Modelling Literature. Blackwell Science Inc.Age and rates of diversification phylogeny in molecular epidemology. Angle Bending.Sequence alignment and homology.2000.Functional constraints and the rate of substitution patterns of codon usage and base composition.Fundamentals of Molecular evolution. M.Molecular phylogenetic archaeology .The universal phylogeny UNIT III Measuring genetic change.Trees and distances. Bond Stretching.kinds of data. .Genetic distance-Measuring evolutionary change on a tree. Force Field Models for the Simulation of Liquid Water.Host parasite co. Roderic D.Methods of reconstruction. Computer Hardware and Software.Modes of evolutionary process. Introduction to Non-bonded Interactions. UNIT II Force Fields. 2. Maximum parsimony methods. Page.Terminology of phylogenetic trees.Molecular clocksevaluation of molecular clock hypothesis . Surfaces.Neutral theory.

Boundaries. Sequence Alignment. Sequence Analysis and Protein Folding. 1997. Phase Space. Molecular Modelling Principles and Application. Some Basic Principles of Protein Structure. BI207 BIOPHARMACEUTICAL TECHNOLOGY Credit : 4:0:0 Marks (40+60) UNIT I Introduction . Molecular Dynamics at Constant Temperature and Pressure. Molecular Dynamics Using Simple Models. Protein Folding and Unfolding. 2. Constructing and Evaluating a Comparative Model. Monte Carlo Simulation of Molecules. Esterification (Alcoholysis) – Halogenation – Oxidation Sulfonation – Complex Chemical Conversions – Fermentation UNIT III Manufacturing Principles . a K UNIT II Important Unit Processes and their Applications .Compressed tablets – wet granulation – Dry granulation or slugging – Direct compression – Tablet presses formulation – Coating – Pills – Capsules n u r a y i n U s r e v y it School of Biotechnology 25 .M. Implementation of the Metropolis Monte Carlo Method. Setting Up and Running a Molecular Dynamics Simulation. Calculation of Simple Thermodynamic Properties. DS Modeling(Basic concepts only) UNIT V Protein Structure Prediction.UNIT III Computer Simulation Methods. Reference Books: 1.Leach. Analyzing the Results of a Simulation and Estimating Errors. Long-range Forces. First-principles Methods for Predicting Protein Structure. UNIT IV Molecular Dynamics Simulation Methods.Haile. J. John Wiley and Sons.Chemical conversion processes – Alkylation – Carboxylation – Condensation and Cyclisation – Dehydration. Longman. 1996. Molecular Dynamics with Continuous Potentials. Monte Carlo Simulation Methods . Models Used in Monte Carlo Simulations of Polymers Molecular Modeling softwares – Insight II. A. Some Theoretical Background to the Metropolis Method.Development of Drugs and Pharmaceutical Industry – Organic therapeutic agents uses and Economics Drug Metabolism and Pharmacokinetics . Predicting Protein Structures by 'Threading.Calculating Properties by Integration. Introduction to Comparative Modeling. Practical Aspects of Computer Simulation. Molecular Dynamics Simulation Elementary Methods.' A Comparison of Protein Structure Prediction Methods: CASP.R.Drug Metabolism – Physico-Chemical Principles – Radio Activity – Pharmacokinetics – Action of drugs on human bodies.

Analysis and Control . BI208 BIOINFORMATICS LAB II Credit : 0:0:2 Marks (50+50) 12 experiments will be notified by the HOD from time to time BI209 INTRODUCTION TO SYSTEMS BIOLOGY Credit : 4:0:0 UNIT I Introduction . Analytical methods and tests for various drugs and pharmaceuticals. Remington’s Pharmaceutical Sciences. 1986. FDA regulations (General) and Indian Drug regulations. Chemoinformatics.A Probabilistic Model of a Prokaryotic Gene and its Regulation Modeling Biochemical Networks .Atomic-Level Simulation and Modeling of Biomacromolecules UNIT III Kinetic Models of Excitable Membranes and Synaptic Interactions . The Use of Molecular Modelling.Vitamins – Cold remedies – Laxatives – Analgesics – Non-steroidal contraceptives – External Antiseptics – Antacids and others. Text Book of Medical Physiology. WB Saunders. Reference books 1.highlights.System-level Understanding of Biological Systems . Hong Kong. Mack Publishing and Co. A C Guyton.Recent advances in the manufacture of drugs using r-DNA technology. placebo effects..Advanced Measurement Systems Modeling Genetic Networks a K UNIT II Modeling the Activity of Single Gene . UNIT V Clinical Trials & Regulations .Clinical Trials – Design. double blind studies. Packing – Packing Techniques – Quality Control . 2.Stochastic Simulation of Cell Signaling Pathways -Analysis of Complex Dynamics in Cell Cycle Regulation n u r a y i n U s r e v y it Marks (40+60) School of Biotechnology 26 .sustained action dosage forms – Parental solutions – Oral liquids – injections – ointments – standard of hygiene and good manufacturing practice. Cerius 2 and QSAR to Discover and Design New Molecules. UNIT IV Pharmaceutical Products. Antibiotics – Biologicals – Hormones – Vitamins – preservation.

Gene Regulation and Metabolism: Postgenomic Computational Approaches. Hiroaki Kitano (Editor). Hamid Bolouri. Foundations of Systems Biology.2002 a K n u r a y i n U s r e v y it School of Biotechnology 27 .Computer Simulation of the Cell: Human Erythrocyte Model and its Application .Software for Modeling and Simulation – E-CELL.UNIT IV Modeling Large Biological Systems from Functional Genomic Data: Parameter Estimation Cellular Simulation . Bower. Computational Modeling of Genetic and Biochemical Networks. Julio Collado-Vides (Editor).2000. V-CELL and GROMOS Reference books: 1.Towards a Virtual Biology Laboratory .MIT Press. Ralf Hofestadt (Editor). 2001 2.Computational Cell Biology : The Stochastic Approach UNIT V Computer Simulation of the Whole Cell . MIT Press. James M.MIT Press. 3.

. Production of monoclonal antibodies. production of vaccines. butanol etc. biopreservatives (Nisin).).SUBJECTS (Biotechnology) Code BT201 BT202 BT203 BT204 BT205 BT206 BT207 BT208 BT209 BT210 BT211 BT212 BT213 Subject Name Basic Industrial Biotechnology Enzyme Engineering and Technology Genetic Engineering Genetic Engineering Lab Bioprocess Principles Bioprocess Lab Plant Biotechnology Bioprocess Engineering Metabolic Engineering Down Stream Processing Down Stream Processing Lab Immunology Immunology Lab Credit 4:0:0 4:0:0 4:0:0 0:0:1 4:0:0 0:0:2 4:0:0 4:0:0 4:0:0 4:0:0 0:0:2 4:0:0 0:0:2 BT201 BASIC INDUSTRIAL BIOTECHNOLOGY Credit : 4:0:0 UNIT – I Introduction to Industrial Bioprocess A historical overview of industrial fermentation process – traditional and modern biotechnology. UNIT – V Production of Modern Biotechnology Products Production of recombinant proteins having therapeutic and diagnostic applications. cellulases etc. lipases. processes. A brief survey of organisms. citric acid. Process flow sheeting – block diagrams. Products of plant and animal cell culture.. UNIT – IV Production of Enzymes and Other Bioproducts Production of industrial enzymes such as proteases.and enzyme inhibitors such as inhibitors of cholesterol synthesis.g.. amino acids (glutamic acid.). UNIT – II Production of Primary Metabolites A brief outline of processes for the production of some commercially important organic acids (e. vitamins and steroids. biopolymers (xanthan gum. aminoglycosides (streptomycin etc. aspartic acid etc. single cell protein. Production of biopesticides. lactic acid. cephalosporin etc.) UNIT – III Production of Secondary Metabolites Study of production processes for various classes of secondary metabolites: antibiotics: betalactams (penicillin. amylases. School of Biotechnology a K n u r a y i n U s r e v y it Marks (40+60) 28 ...). pictorial representation. acetic acid etc. cheese..) macrolides (erythromycin). biofertilisers. phenyalanine. PHB etc. products relating to modern biotechnology.) and alcohols (ethanol.

Lineweaver – Burk Plot. UNIT – III Enzyme Immobilization Physical and chemical techniques for enzyme immobilization – adsorption. Palmer T. Pergamon. concept of active site and energetics of enzyme substrate complex formation. Estimation of Michelis – Menten parameters. Biotechnology: A Textbook of Industrial Microbiology. types of inhibition & models for substrate and product. Monod . pH and temperature effect on enzymes & deactivation kinetics. Agrobios (India). First East – West Press Edition. Immobilised enzyme bioreactors UNIT – IV Purification and Characterization of Enzymes from Natural Sources Production and purification of crude enzyme extracts from plant. Multisubstrate reactions-introduction to ping-pong bi-bi mechanism. Industrial Microbiology. Mechanisms of enzyme action. Presscott. References 1. animal and microbial sources. covalent binding etc. healthcare. Enzymes : Biochemistry... L. advantages and disadvantages. Ellis Horwood Pub. New Age International (P) Ltd. UNIT – II Kinetics of Enzyme Action Kinetics of single substrate reactions. principles of catalysis – collision theory. 3. matrix entrapment. Enzyme Biotechnology. food and environment Text Book 1. transition state theory. Allosteric regulation of enzymes.examples. Dunn. encapsulation. UNIT – V ENZYME BIOSENSORS Application of enzymes in analysis.Text Book: 1. random – order mechanism and compulsory order mechanisms. Biotechnology and Clinical Chemistry. Comprehensive Biotechnology. role of entropy in catalysis. 2. Murrey Moo & Young. BT202 ENZYME ENGINEERING AND TECHNOLOGY Credit : 4:0:0 UNIT – I Introduction to Enzymes Classification of enzymes. Industrial Microbiology.Changeux -Wyman model. methods of characterization of enzymes.E. Wulf Cruger and Anneliese Crueger. Panima Publishing Corporation. specificity of enzyme action. Wiseman. 2004 a K n u r a y i n U s r e v y it Marks (40+60) School of Biotechnology 29 . 2. . development of enzymatic assays. Casida Jr. Turnover number. cross-linking. design of enzyme electrodes and their application as biosensors in industry.

McGraw Hill. NY. restriction and modifying enzymes. Primrose SB. UNIT – IV Polymerase Chain Reaction Inverse PCR. (Kunkel’s Method). Brent R. James. Diagnostic importance. Lee. Greene Publishing Associates. Insect.Sanger’s method. prokaryotic and eukaryotic expression vectors. Text Book 1. vaccines. Harvey W. Invitro fertilization. UNIT – II Creation of Recombinant Molecules Restriction mapping. 1. Sambrook et al Vol. Blanch. embryo transfer technology. Nested PCR. E. Taqman assay. Academic Press. "Current Protocols In Molecular Biology ". An Introduction To Genetic Engineering ". Biochemical Engineering Fundamentals. Screening of libraries with DNA probes and with antisera. Berger Sl. RAPD. “Molecular Cloning” n u r a y i n U s r e v Marks (40+60) y it School of Biotechnology 30 . Douglas S. methods of nucleic acid sequencing. PHI. "Principles Of Gene Manipulation. transgenic and knockout animals. Marcel Dekker. genetic elements that control gene expression. James M. a K Reference books 1. Kimmer AR. Vol 152. design of linkers and adaptors. Blackwell Science Publications. safety guidelines of recombinant DNA research. 2. Biochemical Engineering. Bailey & David F. 3. 1993. UNIT – V Applications of Recombinant DNA Technology Cloning in plants. Biochemical Engineering. Recombinant cytokines and antibodies. Site Directed Mutagenesis.References 1. Ollis. 1987 3. Clark. stem cell therapy. Old RW. Characteristics of plasmid and phage vectors. 2. 1-3. BT203 GENETIC ENGINEERING Credit : 4:0:0 UNIT – I Basics of Recombinant DNA Technology Role of genes within cells. gene-therapy. 1988. Moore DD. UNIT – III Construction of Libraries Construction of cDNA and genomic libraries. "Methods In Enzymology". USA. Yeast and Mammalian vectors. RACE PCR. Molecular beacons. Inc.Ansubel FM. Kingston RE.

air sterilization and design of depth filters. Principles of Fermentation Technology. medium requirements for fermentation processes. oxygen consumption and heat evolution in aerobic cultures.batch. Ltd. maintenance coefficients energetic analysis of microbial growth and product formation. Stanbury. design of various commercial media for industrial fermentations – medium optimization methods UNIT – III Sterilization Kinetics Thermal death kinetics of microorganisms. Text books 1.BT204 GENETIC ENGINEERING LAB Credit : 0:0:1 Marks (50+50) 6 experiments will be notified by the HOD from time to time BT205 BIOPROCESS PRINCIPLES Credit : 4:0:0 Marks (40+60) UNIT – I Overview of Fermentation Processes Overview of fermentation industry. yield coefficients of biomass and product formation. nitrogen. Simple unstructured kinetic models for microbial growth. available electron balances. filter sterilization of liquid media. general requirements of fermentation processes. minerals. fed batch and continuous cultivation. design of sterilization equipment . main parameters to be monitored and controlled in fermentation processes. Shuler. substrate and product inhibition on cell growth and product formation. UNIT – IV Metabolic Stoichiometry and Energetics Stoichiometry of cell growth and product formation.. and Kargi. Stephen J. Second Edition. Peter F. degrees of reduction of substrate and biomass. UNIT – V Kinetics of Microbial Growth and Product Formation Modes of operation . basic configuration of fermentor and ancillaries.F.batch and continuous. Hall & A.. UNIT – II Raw Materials and Media Design for Fermentation Process Criteria for good medium.Basic concepts – Second Edition Prentice Hall of India Pvt. oxygen requirements. thermodynamic efficiency of growth. growth of filamentous organisms. Butterworth – Heinemann An Imprint of Elsevier India Pvt. batch and continuous heat sterilization of liquid media. elemental balances.L. 2005 2.leudeking-piret models. product formation kinetics . Ltd. carbon. Monod model. 2005 References a K n u r a y i n U s r e v y it School of Biotechnology 31 . " Bioprocess Engineering . vitamins and other complex nutrients. examples of simple and complex media. Whitaker. M. medium formulation of optimal growth and product formation.

School of Biotechnology 32 . 3. Unit V: Transgenics Stress tolerance . Unit III : Developmental Aspects of Rhizobium Legume Symbiosis.. BT208 BIOPROCESS ENGINEERING Marks (40+60) a K n u r a y i n U s r e v y it Marks (40+60) Credit : 4:0:0 UNIT – I Analysis of STR Stirred tank reactor . 1985. McGraw Hill (2nd Ed. 3. Biochemical Engineering. Marcel Dekker.non-ideality.. tanks in series and dispersion models – application to design of continuous sterilizer. Regulation of nif and nod genes. 1989. 1986. Mantal S. Symbiotic Nitrogen Fixation. Dodds J. Mickee R. Unit II : Plant Viruses Classification. factors influencing disease resistance and susceptibility RFLP. Pauline Doran.H. An Introduction to Genetic Engineering in Plants. Principles of Plant Biotechnology. Cambridge University Press.A.1. Plant Genetic Engineering. Revolution in Biotechnology. Blackwell Scientific Publication. Harvey W.. Bailey and Ollis.. Douglas S. Types of plant vectors and their use in gene manipulation.. diagnosis – remedy – viruses as a tool to deliver foreign DNA. 2.A. Blackwell Scientific Publications.H. Unit IV: Molecular Aspects of Disease Susceptibility and Resistance Transposable elements. Inc. Pests and insects resistance – viral resistance – development of disease resistant plants by introducing Bacillus thuringiensis genes.). " Biochemical Engineering Fundamentals". 1985.L.Biotic and abiotic temperature. Bioprocess Engineering Calculation. Clark. 2. Cambridge University Press. Mathews J. RTD and stability analysis. Blanch. Text & Reference Books: 1. BT206 BIOPROCESS LAB Credit : 0:0:2 Marks (50+50) 12 experiments will be notified by the HOD from time to time BT207 PLANT BIOTECHNOLOGY Credit : 4:0:0 Unit I: Agrobacterium and Plant Genetic Engineering Agrobacterium mediated gene transfer and cloning. salinity. Marx J. drought etc.

Regulation of RNA synthesis by amino acid. Biochemical Engineering. power consumption and impeller tip speed. feedback resistant mutants. James E. Handbook of Bioreactors. group transportation. Scale up criteria for bioreactors based on oxygen transfer. References 1. Ollis. limiting accumulation of end products. James M. RTD and stability analysis. fluidized bed reactor bubble column reactors – nonideality. formulation of dimensionless groups and calculation of effectiveness factors. airlift reactor. Atkinson. methods for the determination of mass transfer coefficients. Harvey W. regulation in branched pathways. Blanch. 3. oxygen mass transfer in bioreactors . UNIT – II Synthesis Of Primary Metabolites Alteration of feed back regulation. Clark. Bailey & David F. models of cellular energetics and metabolism. permeability control: passive diffusion.cAMP deficiency. Credit : 4:0:0 a K UNIT – I Introduction Jacob Monod model for gene expression regulation-Lac operon. mass transfer correlations. PHI. Biochemical Engineering Fundamentals. Lee. UNIT – III Bioreactor Scale – up Regime analysis of bioreactor processes. alteration of permeability for metabolites. steady and transient culture metabolism. Anton Moser. active transport. Design of immobilized enzyme reactors – packed bed. concerted feed back regulation. 2.differential regulation by isoenzymes. Biochemical Engineering. Marcel Decker Inc. UNIT – IV Modelling and Simulation Of Bioprocesses Study of structured models for analysis of various bioprocess – compartmental models. catabolite regulationglucose effect. Feed back regulation. n u r a y i n U s r e v y it BT209 METABOLIC ENGINEERING Marks (40+60) School of Biotechnology 33 . USA. Dynamic simulation of batch. . McGraw Hill. plasmid replication and plasmid stability model. Kinetics and Reactors”. UNIT – V Bioreactor Consideration in Enzyme Systems Analysis of film and pore diffusion effects on kinetics of immobilized enzyme reactions. “Bioprocess Technology. 4. fluidized bed and membrane reactors.microbial oxygen demands. single cell models. Springer Verlag. cumulative feed back regulation. Douglas S. Text Book 1.UNIT – II Analysis of Other Configurations Packed bed reactor. fed batch.

2005 References 1. 1980. ionexchange.. Cooney C. " Biochemistry ". factors important to bioconversion. dialysis. feedback repression. Stephen J. yields. enzyme induction. 2. improving fermentation. idiophase relationship.UNIT – III Biosynthesis Of Secondary Metabolites producers of secondary metabolites. Whitaker. co-metabolism. UNIT . bioaffinity and pseudo affinity chromatographic techniques. catabolite repression. BT210 DOWNSTREAM PROCESSING Credit : 4:0:0 UNIT – I Downstream Processing Introduction to downstream processing principles characteristics of biomolecules and bioprocesses. avoidance of product inhibition. permeability. conversion of insoluble substances.C. regulation of enzyme synthesis. reverse phase..filtration and centrifugation. mutants resistant to repression. aqueous two-phase extraction. Zubay G.E. Demain A.. trophophase. Humphery A.. liquid-liquid extraction. John Wiley And Sons. Hall & A.. mixed or sequential bioconversions. Ltd.D. UNIT . recognising growth cycle peak. precipitation of proteins by different methods. gene dosage.L. catabolite regulation by passing control of secondary metabolism. a K UNIT – III Isolation of Products Adsorption. membrane separation – ultrafiltration and reverse osmosis. drying and lyophilization in final product formulation. adsorption. 1989. " Fermentation And Enzyme Technology ".I.V Regulation Of Enzyme Production Strain selection. enzymatic and chemical methods. Butterworth – Heinemann An Imprint of Elsevier India Pvt. Peter F. UNIT – IV Product Purification Chromatography – principles. Second Edition. Wang D. Text books 1. Stanbury. instruments and practice. Macmillan Publishers. mutation. Lilly M. Cell disruption for product release – mechanical.IV Bioconversions Advantages of bioconversions. n u r a y i n U s r e v y it Marks (40+60) School of Biotechnology 34 . UNIT – II Physical Methods of Separation Unit operations for solid-liquid separation . Precursor effects. induction.P. Dunnil.L.. Principles of Fermentation Technology.. size exclusion. UNIT – V Final Product Formulation and Finishing Operations Crystallization. specificity. hydrophobic interaction.. feedback regulation. Pretreatment and stabilisation of bioproducts..

regulation of T-cell and B-cell responses. VCH Pub. 1989. antigenantibody reactions. antigen presenting cells.L. bacteria. primary and secondary lymphoid organs. non-isotopic methods for detection of antigens. haptens. maturation. Autoimmune disorders and diagnosis. resistance and immunisation. 2. innate and acquired immunity. J. monoclonal and polyclonal antibody production a K n u r a y i n U s r e v y it Marks (50+50) Marks (40+60) School of Biotechnology 35 . AIDS and Immunodeficiencies. immune responses to infections: immunity to viruses. antibodies: structure and functions. E. Vaccines. TCR. antigens: chemical and molecular nature. RIA. Belter. Cussler And Wei-Houhu – Bioseparations – Downstream Processing For Biotechnology.O. Wiley Interscience Pun. R. activation and differentiation of T-cells and B-cells. 2.) – Protein Purification – Principles. immunohistochemistry. UNIT – II Cellular Responses Development. Ryden. Jenkins.A. High Resolution Methods And Applications. UNIT – III Infection and Immunity Injury and inflammation. Narosa Pub. types of immune responses.Text Book 1. P. fungi and parasites. Scopes – Protein Purification – Principles And Practice. (Ed.K. laws of transplantation. Janson And L. theory of clonal selection. (Ed. complement. R. antibodies: genes and generation of diversity. antigen processing and presentation. adjuvants. tolerance. UNIT – V Immuno-Techniques ELISA. allergy and hypersensitivity. Immunoelectrophoresis. Butterworth-Heinemann (1992). major histocompatibility complex. (1994). SDS-PAGE. (1988). cytokines. UNIT – IV Transplantation and Tumor Immunology Transplantation: genetics of transplantation. tumor immunology. chemiluminescence assay. References 1. Autoimmunity. monoclonal antibodies: principles and applications.) – Product Recovery In Bioprocess Technology – Biotechnology By Open Learning Series.C. immunosuppression. BT211 DOWNSTREAM PROCESSING LAB Credit : 0:0:2 12 experiments will be notified by the HOD from time to time BT212 Credit : 4:0:0 IMMUNOLOGY UNIT – I Introduction Cells of immune system.

. 1998. Ashim K. TataMcGraw-Hill. Chakravarthy. Brostoff. Kuby J. BT213 IMMUNOLOGY LAB Credit : 0:0:2 12 experiments will be notified by the HOD from time to time a K n u r a y i n U s r e v Marks (50+50) y it School of Biotechnology 36 . 2002. 3.. Immunology. 4 Edition. Tizard. Immunology. Roitt I. 2. Male. WH Freeman & Co. 2000.. Immunology. Reference books 1.Text Book th 1. Immunology. Mosby Publ.

Basic Structures. gums & polysaccharides. Phospolipids. Components of Fatty acids. properties and preparation of Pectic substances.1 Processing of Food –1 Analysis of Food Constituents – 2 Food Additives Lab Analysis of Food Commodities – 1 Credit 3:1:0 4:0:0 4:0:0 3:1:0 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 0:0:2 0:0:2 0:0:2 0:0:2 4:0:0 FP201 FOOD CHEMISTRY Credit : 4:0:0 Unit I : Water and ice Importance of water in foods. Auto oxidation and hydrolysis. Chemistry of fatty acids & glycerides. . Cellulose. Rancidity of fats & oils.Sorption Phenomena and Sorption isotherms. shortening agents. examples – Dispersed systems – some basic considerations Chemistry of Carbohydrates: Nomenclature Classification & structure of carbohydrates.SUBJECTS (Food Processing) Code FP201 FP202 FP203 FP204 FP205 FP206 FP207 FP208 FP209 FP210 FP211 FP212 FP213 FP214 FP215 FP216 Subject Name Food Chemistry Engineering Properties of Foods Fruit and Vegetable Technology Food Additives Dairy Technology Cereal Technology Sugarcane & Confectionery Technology Meat Technology Plantation Products and Spices Technology Food Packaging Technology Milling and Bakery technology Analysis of Food Constituents . Physical & chemical properties of sugars Unit II: Chemistry of Polysaccharides Chemistry. maltodextrins. Concept of bound & free water & their implications. and its prevention. and unsaponifiables. Physical & chemical characteristics of fats & oils. Chemical reactions of carbohydrates. Structure of water & ice. confectionary fats etc. antioxidants. hydrogenated fats. Process flow sheet for the manufacture of edible oils (refined and hydrogenated) n u r a y i n U s r e v y it Marks (40+60) School of Biotechnology 37 . Cyclodextrins a K Unit III : Chemistry of Lipids Definition & classification of lipids. Starch and its hydrolytic products.

Pectic Enzymes. Sources and distribution of Proteins. T. (1960) 3. M : Biochemistry Of Foods Harcourt Publishers Ltd. Rheological models for visco-elastic fluids. H. Nomenclature. Xanthine Oxidases. A.Unit IV : Chemistry of Proteins Importance of Proteins. Proteases. J. Eskin. L. Lipoxygenases.Sikroski: Chemical and functional Properties of Food Components: Technomic Publishing company.3rd Edition (2004) Springer Verlag March 2004 4. Catalases. H-D Belitz. N. Physical & chemical characteristics of Proteins. classification & nomenclature of enzymes. effect of concentration on viscosity. structure and chemistry of amino acids. Owen R Fennema : Food Chemistry – III edition Marcel Dekkar Inc. Lancaster. carnitine Text Books and Reference Books: 1. West port. John M Demann: Principles of Food Chemistry AVI Publishing Co.Meyer : Food Chemistry Van Nostrand Reinhold Co. Pieter Walstra: Physical Chemistry of Foods Marcel Dekker Publishing. Immobilized enzymeOne example of working of each enzyme.Robinson: Food Biochemistry and Nutritional Value Longman Scientific and Technical Publishers.S. New York. New York (2003) 10. classification. Enzymes: Introduction. USA 1997 6. Glucose Oxidases. Specificity.B. Zdzislaw E. Denaturation. USA (1987) 8. With Henderson. effect of Temperature on Rheological properties. Sikorski: Chemical and Functional Properties of Food Components Technomic Publishing. USA (Edition #2. peptides & Proteins. Nature.Phenolases. identification & purity of Proteins. USA (1997) 11. USA (1984) a K n u r a y i n U s r e v y it Credit : 4:0:0 FP202 ENGINEERING PROPERTIES OF FOODS Marks (40+60) Unit I : Rheological Properties of Liquid Foods Rheological Classification and models. Isolation & purification of enzymes & their importance. Amylases. David S. Unit V : Chemistry of Vitamins Summary of vitamin stability – Toxicity and sources of vitamins – Bioavailability of vitamins – Reasons for the loss of vitamins in foods – Fat-soluble and water soluble vitamins – Choline. USA 1974 5. Isolation.H. USA (1963) 9. measurement methods. Assay Techniques. Viscometers and Rheometers of different design and their applications School of Biotechnology 38 .P.Coulate: Food – Chemistry of its Components Royal Society of Chemistry. Oxidoreductases. 2. Function.Breverman: Introduction to Biochemistry of Foods Elsevier Publishing Company. Peroxidases. New York (1996). W Grosch and P Schieberle: Food Chemistry . Zd Zislaw and E. 1990) 7.

M. Polar solvents. J.Rizvi: Engineering Properties of Foods Mercel Dekker inc. Sorption energetics. Constant and falling rates of drying of foods.Rao and S.H. Hardness and brittleness of grains. M. Measurement of thermal properties like specific heat. relaxation. measurement of water activity. Ionic solutions. P. Unit IV : Diffusion of gases in Liquid and Solid Foods Moisture transfer in foods. Prediction of food dielectric properties. electric energy transmission properties. Diffusion in porous foods. Dielectric measurements. Nuri N. Thermal properties of frozen foods. R. Inter-phase moisture transport. flow of powders and grains in chutes an channels. Unit V : Electrical properties of foods Dielectric properties.K. Unit III : Thermal Properties of Foods Definitions . enthalpy. methods of measurements. Reynold Jewitt and Others: Physical Properties of Foods Allied Science Publishers (1983) 4. Moisture sorption isotherms and their applications. New York (1998) 2. stress-strain diagrams.specific heat. conductivity and diffusivity.Bagley: Physical Properties of Foods AVI Publishing company Inc.Lewis: Physical Properties of Foods and Food Processing Systems Woodhead Publishing Cambridge. Modelling thermal properties of frozen foods. Text/reference Books 1. Rheological models. conductivity and diffusivity. Mohsenin: Physical Properties of Plant and Animal Materials Gordon and Reach Science Publishers (1970) 8. stress and strain in solid foods. compaction and emptying problems of floury foods in silos and bins. Fugacity and activity. surface heat transfer coefficient. Electro-magnetic field effects. UK (1990) 3. Concept of equilibrium moisture content in foods. Predicting thermal properties.A. Water activity in foods. different models and their applications. a K n u r a y i n U s r e v y it School of Biotechnology 39 . Westport USA (1983) 7.J.S. texture measuring instruments. Jodhpur. New York (1995) 5. Creep. Dynamic testing of solid foods. Shafiur Rehman: Food Properties Hand Book CRC Press Inc. Diffusion of aroma components Applications of diffusion and migration in food packaging. enthalpy.Gupta: Physico-Chemical Constituents and Engineering Properties of Food Crops : Scientific Publishers. Micha Peleg and Edward B.Kachru and R.H.Unit II : Rheological Properties of Solid Foods Static tests for solid foods.Prentice: Measurements in the Rheology of Food Stuffs Elsevier Applied Science Publishers (1984) 6. Thermodynamic Properties of Foods in Dehydration: Thermo dynamics of food-water systems.

Squashes etc. Different kinds of evaporators used for concentration of fruit juices and pulp. Cross Flow Shelf Dryers. Hydraulic Pressing and Clarification. preservation treatments for freshly harvested fruits and vegetables. grating. Clarification centrifuges – Decanters and desludgers. Packaging of Dried slices. Dices and powder. General methods of preservation of whole fruits/Vegetables and processed fruits and vegetables. Post harvest field operations. Jellies. closing and sterilization operations. slicing dicing. Preparation of Fruit Powders. deseeding etc for each product. Preparation of product for dehydration. Tubular. a K n u r a y i n U s r e v y it School of Biotechnology 40 . Pickling operations. Tomato products – Hot and Cold Break processes. Slicing/Dicing. Unit IV Dehydration Dehydrated products. Canning operations. Merits and demerits of Freeze Drying. Potato. Brief study of Hurdle technology as applied to Vegetable and Fruit processing. Fruit Bars and Fruit powders. sorting and Storage operations for fruit and vegetables. among fruits. Viscosity problems. Fruit juice concentrates. Marmalades. Pineapple. Preparation of Fruit material for powder production. Working of Spray Dryer and Drum Dryer. Common machinery for operations like Peeling. Tomato Deseeding and clarification. Pickles. Grapes etc. Hot water and Steam Blanching. Preparation of vegetables for canning operations. Freeze Dryers. peeling. Freeze drying Principles. Dehydration principles and equipment used for drying. Pulping. Fruit juice aroma Recovery and its importance. Grating. Different preservatives used for long and shortterm storage.Credit : 4:0:0 FP203 FRUIT AND VEGETABLE TECHNOLOGY Marks (40+60) Unit I Introduction Production of Fruits and vegetables in India. in plastic and SS containers. among Vegetables. Flow diagram in each case. Preparation for canning. – Washing. Juice and pulp extraction and clarification. Different types Glass and Plastic Containers. Spoilage factors. Precautions in Canning operations. Unit II Canning of Fruits and Vegetables Commercial products from each of the fruits and vegetables. Large capacity storage containers in plastic. Bulk preparation and storage of Mango pulp. Papaya. Guava. Mango Pulp extraction and concentrations. Importance of Blanching operations. Composition of each of the major fruits and vegetables produced in the country-Mangoes. Different filling. Minimal Processing and packaging of vegetables. Clarification of juices. Preparation and packaging of Pickles. Brief on Aroma Recovery equipment. Ketchup. Tomatoes. Dried Vegetable Slices and Dices. Vacuum Shelf Dryers. Onion Brinjal etc. Puree. Cause for heavy losses. Simple estimation of drying costs. Unit III Processing of fruit juices Preparation of specialty products like. Sauce. Beans. Plate and scraped surface evaporators. Bottled Products: Preparation of products like Jams. Reception. Canning of Vegetables. Carrot. Packaging of whole fruits and vegetables for internal and export markets. Batch and Continuous Blanching.

Pulps and Puree Brief information on Asepticity and how it is strictly maintained in the plant. Different system of filling practiced. Composition.. G. Salunkhe. Y. Srivastava.. teratogenicity – PFA.P.: Fruit and Vegetable Preservation: Principles and Practices. 1996 a K Credit : 3:1:0 Unit I Introduction – food additives definition – Determination of the limit for addition – NOEL – Toxicity data – Method of determining toxicity – LD50. Mujumdar A. principles and practices.E. Manorajan Kalia and Sangita Sood. Siddappa..: International Book Distributing Co. chemical properties. carcinogenicity. (1993) 9. (1995) 8. Srivastava. Minimal processing – Basic concepts. Composition and Processing. S.K. India. New York. New York. G. Production. Aseptic heat exchangers for sterilizing and concentrating the product. and Kadam. Noyes Data Corporation. Chakraverty. New Jersey. Tetra pack for small quantities. Dauthy.K. and Sakal. International Book Distributing Co.E. Lal. W. levels of additions in individual products.: Tropical Fruit Processing Academic Press. (1986) 5. Aseptic fillers. FPO regulations – GRAS additives Unit II Types. Lucknow. Salunkhe. (1997) 2.S.Hui and Others: Hand Book of Vegetable Preservation and Processing Mercel Dekker New York 2004 6. and Sanjeev Kumar: Fruit and vegetable preservation. Handbook of Post-harvest Technology: Marcel Dekker Press. Seymour. Storage of Aseptically packed products. Raghavan G. 1988) 4. D.H. Indian Council of Agricultural Research. Dole system and Scholle system for bulk storage in Bag & Boxes and Bag & Drums.T. (1995) 7. Jagtiani J. J. and Kadam.S. Chan. Lucknow.: Handbook of Fruit Science and Technology: Production. 1998 12. M.: Fruit and Vegetable Processing..P: Commercial Processing of Vegetables. Lucknow (2nd Edition 1998).S. Hamson. S.R. and Tucker. R. USA (2001) 11. A. Chapman and Hall. : Preservation of Fruits and Vegetables.L. New Delhi. S.A: Biochemistry of Fruit Ripening.: Handbook of Vegetable Science and Technology.Unit V Aseptic and other methods of processing Aseptic processing and Bulk packing of Fruit juice concentrates. and Kumar.P. Taylor. G. toxicity data of Acidulants – Preservatives – Emulsifiers and gums . FDA.S. Storage and processing Marcel Dekker. and Tondon G.V and Ramaswamy H.S. 10. London.Antioxidants n u r a y i n U s r e v y it FP204 FOOD ADDITIVES Marks (40+60) School of Biotechnology 41 .. London.. Reverse osmosis Text/Reference Books 1. International Book Distributing Co. (1975) 3. Food Preservation and Processing.. L. (Ed. D. Marcel Dekker.. Kalyani Publishers.S. H. G. Ludhiana.B.

Manufacture of paneer. antibrowning agents. Handbook of Food additives by Thomas Furia. Salminen S. Milk powder – Quality aspects Unit IV Manufacture of Ice Cream and other Dairy Products Manufacture of Ice cream – Chemistry and technology –Microbiology of ice cream . Processing flow sheet. Sweetened Condensed milk. chemical properties. food value. levels of additions in individual products. Chelating agents. Milk transport and storage in dairy plants.Quality aspects Manufacture of Butter. Cream separating Centrifuges. composition. contaminants. Pumps. Packaging of milk in bottles and sachets. Food additives by Brannen A. Revised and Expanded. Vacuum Evaporators. Toned Milk. Cholesterol. Defects in cheese. toxicity data of Colourants – Natural and artificial. composition and industrial uses a K n u r a y i n U s r e v Marks (40+60) y it School of Biotechnology 42 . Second Edition. 2002. toxicity data of Dough conditioners . Marcel dekker Inc.flour improvers – Humectants –Enzymes. chemical properties.Unit III Types. Milk Chillers.L. milk reception in dairies and tests. Cell count and other tests. Starches Unit IV Types. Plant piping. Nutritional additives Text book 1. chemical properties. Spray and Drum Dryers. Basic dairy terminology.Quality aspects Extraction of casein from milk. Davidson P. Ice Cream Freezers. fatty acids and their relation to cardiovascular diseases – Dietary recommendations – Applications of enzymes in dairy industry Unit II Dairy Processing Equipments Milk processing terminology. levels of additions in individual products. Product instantizing equipment. Unit III Manufacture of Dairy Products Cheese – Types of cheese. milk as raw material. toxicity data Sweeteners – Natural and synthetic. USA.H.. Fat substitutes and replacers Unit V Types. Khoa.M. Pasteurisers (Heat Exchangers). 2. Homogenizers. FP205 DAIRY TECHNOLOGY Credit : 4:0:0 Unit I Dairy Chemistry and Microbiology Introduction. and Thorngate J. Ghee – Grading of butter . levels of additions in individual products. Bottle and pouch fillers. properties. Quality and Quantity tests at reception.. Equipment employed. Flavourants. Flavour enhancers.

2. Bran and Brokens separators. Westport. cost of energy. Shellers. Germany (1981) 8. Control of energy losses and Energy conservation. W. Engelberg Huller Mills. Curing of Paddy. Kessler: Food Engineering and Dairy Technology Verlog Kessler Publishing House. Use of Rice Bran in Edible oil Industry. Allahabad. (1998) 4. Parboiling Processes. Polishers-Cone polishers and other types. H. activated carbon. Components of modern Rice mill. James Harper and Carl W.M. Paddy Separators – Satake and Schule Designs.Paddy husk and its uses .as boiler fuel. N. NIIR Publications. sources of energy. 2004. Farral: Engineering of Dairy and food Products (II Edition 1970) Robert E. USA (2005) FP206 CEREAL TECHNOLOGY Credit : 4:0:0 Marks (40+60) a K Unit I Paddy Processing: Paddy Varieties. Under Runner Shellers and Centrifugal Shellers. Krieger Publishing Co. Rice Mill yields and loss due to brokens at different stages of milling. Modern Rice Mills. India. National Institute of Industrial Research. Text books and Reference books 1. furfural and other by products. Production of Flattened Rice and Puffed Rice from Paddy Unit II Rice Milling: Paddy Dehusking Processes. Edger Spreer & Axel Mixa: Milk and Dairy Product technology Mercel dekker Inc. Hall: Dairy Technology and Engineering AVI Publishing. Curd.Production of lactose and whey Unit V Fermented dairy products Fermented products – Yoghurt. Rice Mill machinery handling. Prof. II Edition. Their Composition and Quality characteristics. India (1985) 3. n u r a y i n U s r e v y it School of Biotechnology 43 .G. Cold Water soaking and Hot water soaking processes. By Products of Paddy Processing .Y. Modern Technology of Milk processing and Dairy products. USA (1976) 9. Rice Mill Flow Chart. W. Arthur W. Tufail Ahmad: Dairy Plant Systems Engineering Kitab Mahal. CRC Press (2003) 6. Clunie Harvey and Harry Hill: Milk Products Bio Tech Books. Pre Cleaners. Paddy Dryer-LSU Dryer. acidophilus milk etc Energy use in Dairy plant. Edger Spreer: Milk and Dairy Product Technology Mercel Dekkar Inc. New Delhi (1999) 7. New York. husk ash. Garret Smit : Dairy Processing (Improved Quality) Woodhead Publishing Ltd. NY 5.

conditioning. and their applications. Problems of Dust Explosion in Grain Storages.Evans: Technology of Cereals (4th Edition) Elsevier Science (Pergaman). Bag Storage structure design. Fumigation Processes for bag storage piles. merits and demerits. Pulses and Oilseeds. concrete and Metal Silos. Traditional Processing steps – Pre-cleaning. Cleaning. Merits and demerits.Unit III Milling of Pulses: Major Pulses grown in the country and their application. Ruth H. Dehusking and splitting. Aspiration.L. USA (1989) n u r a y i n U s r e v y it School of Biotechnology 44 . Bran and Fibre separation. Degermination.Kent and A. process flow chart. Mini dal mill. Silo flow problems. Oxford. Modern milling process. Gluten and Starch Separation. Conveyors and Elevators for feeding and discharging into Silos In silo Aeration and Drying. their applications. Protection against Rodents. losses during milling. Relative Costs of Silo and Bag Storage. Dunnage. Sun drying. lay outs. Extraction and refining of Corn oil in brief. Acid Hydrolysis. merits and demerits. working principle. Oxford and IBH Publishing Co. Materials for Dunnage. (1994) 4. Pneumatics in a maize mill. pulse flour products. Samuel Matz: The Chemistry and Technology of Cereals as Food and Feed. extraction process. mass balance. Enzyme Hydrolysis. Steeping. Pallets. Chakraverty.: Post Harvest Technology of Cereals. Malto Dextrin and other products. Roller milling. Technology and Nutrition Mercel Dekker Inc. Cover Plinth Storage Structures. Construction of Silos. Problems of Silo storage. Purifying. splitting of pulses in Pulse splitter. Semolina. Plans for Bag storage. Mechanical hot air drying and conditioning. Wet milling of Maize and corn: Modern methods of processing. Processing for Dextrose. Sifting. Unit V Grain Storage and Handling: Bag Storage. Quality Changes of Grains during storages and remedial measures to prevent unwanted quality changes. Advantages and Disadvantages. Status of Pulse milling industry in India. CAP storage (Ceiling and Plinth Storage). Parameters of good storage structure. need for modernization. Pitting. Isomerization processes. Dehusking in Pulse Pearler. Machinery and equipment employed.D. Grinding of split pulses. N. advantages and disadvantages. Traditional milling process. Pre-cleaning. Relative merits and demerits of Silo storage to Bag Storage. Products of milling-Flour. Degermination and Dehusking. UK. London (1982) 5. Debranning and starch separation. Chapman & Hall (1992) 3. Water conditioning. Matthews: Pulses – Chemistry. Brewers’ grits etc. Starch conversion into other value added products. Physical load and mechanical strength of Silos. A. Pests and Mites. Oil application. Drying of legumes. George E Inglett: Maize-Recent Progress in Chemistry and Technology Academic Press. equipment used. Bulk Storage in silos and large Bins. Unit IV Milling and Processing of Maize: Dry milling of maize: Storage and drying. cleaning equipment. Calcutta (1995) 2. Fungi. Equipment needed for Degermination. a K Text/reference Books 1.

6. rum and gin – Quality aspects Unit IV : Technology of Carbonated Beverages and Confectionery Manufacture of carbonated beverages – quality aspects – Manufacture of sugar-free. Y. Properties of Granulated sucrose and Liquid Sugars. Invert sugar and their characteristics. Karel Kulp and Joseph P Pante:Hand Book Of Cereal Science and Technology Mercel Dekkar USA (2000) FP207 SUGARCANE AND CONFECTIONERY TECHNOLOGY Credit : 4:0:0 Unit I Introduction Sugarcane and beet as sugar raw materials. sugarless carbonated beverages. Back strap Molasses and its uses. New York (1987) 8. pH control. lollipops. Treatment of clarified juice. Crystallization. /Sugar balance. London (1980) 7. drying and uses of Bagasse. Manufacture of high boiled sweets – Ingredients. Extraction of juice. Unit III : Technology of Alcoholic Beverages Manufacture of beer and wine and champagne . Pomeranz: Modern Cereal Science and Technology VCH Publishing Inc. Cryde M. Purification of juices-juice filtration and chemical purification. Bernard Godon and Claude Willm: Primary Processing of Cereals Berns and Noble Publishers (1994) 10. Lime addition. Smartt: Tropical Pulses Longman Group Ltd. St. Sugar analysis. extraction yields. Christensen: Storage of Cereal Grains and their Products American Association of Cereal Chemists inc. USA 1982 9. Sugar Refining. Clarification stages. Washing of sugar crystals and centrifugal separation/dewatering of sugar and other related processes. coextruded products Manufacture of gums and jellies – Quality aspects a K n u r a y i n U s r e v Marks (40+60) y it School of Biotechnology 45 . J. evaporation – multiple effect evaporators. energy conservation. Vacuum pans. Sugar plant sanitation. Methods of manufacture – Types – Center – filled. Confectionery manufacture General technical aspects of industrial sugar confectionery manufacture.Quality characteristics Manufacture of distilled beverages including whiskey. Flow charts for manufacture of Granulated sugar and Liquid sugars. Paul. Applications in animal feed Unit II Sugar production process details: Energy and material balance of cane sugar process. Specialty products of Sugar Industry.. brandy. Sugar recovery –improvement.

meat processing equipment. Maurice Shachman. slaughtering equipment. Packaging of various poultry products a K n u r a y i n U s r e v Marks (40+60) y it School of Biotechnology 46 . Ante mortem handling and welfare of animals. Chemists and Technologists: AVI Puvblishing. bleeding. Offal handling and inspection. E. Sticking. West port (1973) 4. Inedible by products: Carcass processing equipment. Second edition. design of handling facilities. Water and Electric energy requirement for abattoir operations. London (1986) FP208 MEAT TECHNOLOGY Credit : 4:0:0 Unit I : Meat. Washing area.Ray.International Consultants. New Delhi 7. Junk & Harry M. Cooked and Refrigerated meat. Pancost: Hand Book of Sugars – for Processors. Operational factors affecting meat quality. effects of processing on meat tenderization. Guilford L Spencer and George P. description of bone and its modifiers. London 6. Dried and preserved meat. Description of animal fat and its modifiers. Cured meat. W. Beef/Sheep and Pig Dressing operations. Great Britain 2. CRC press. Production methods for Intermediate moisture and dried meat products. Definitions and measurements. Lozenges. Countlines . Fish and Poultry – Chemistry and Microbiology Meat composition from different sources. Soft Drinks Companion: A Technical Handbook for the Beverage Industry. P. Hoisting rail and traveling pulley system. Unit III Meat Products: Canned meat.. Jackson: Sugar Confectionery Manufacture. Toffee and fudge – Liquorice paste and aerated confectionery. E. Florida. Post mortem muscle chemistry. 1999. Aspen publishers Inc. sugar pannings and Chewing gum. and stunning methods.Unit V : Manufacture of Miscellaneous Products Caramel.Hugott: Hand Book of Cane Sugar Engineering Elsevier Publishing /company. Prepared meat products. Meat plant hygiene. Made: Cane Sugar Hand Book (1993) John Wiley and sons Inc. (1950) 5.Quality aspects Text/Reference Books 1. Oliver Lyle: Technology of Sugar for Refinery Workers Chapman and Hall Ltd. Manohara Rao: Industrial Utilization of Sugar Cane and its co-products P... Typical lay outs. Meat colour.J. meat microbiology and safety Unit II Slaughtering and Carcass Processing Modern abattoirs and some features. electrical gadgets and manual gadgets. Different kinds of sausages –Equipment used for all the process operations. flavors of meat products. dressing.B. Good manufacturing practice and HACCP. Explanation of muscle structure and compositions and its modifiers. Frozen meat. USA (2005) 3. stunning pen.

their manufacture. Poultry meat processing operations in detail along with equipment used – Defeathering. New York (2001) 5. Transportation in Refrigerated trucks. Wheaton and T. weighing. Scalding etc. Lay-out and design of poultry processing plants. Use of dry ice and liquid nitrogen as preservation elements.H. Deodorization of transport trucks. Plant sanitation. Egg yolk products. Packaging of poultry products. by products – eggs. George J. Product export and its sustenance. pickling and preparation of fish protein concentrate.K. egg products. Frank Gerrard: Meat Technology Northwood Publications. G Mead (Editor): Poultry meat processing and quality Woodhead Publishing Limited (2004) 8. USA 1984 3. a K Text/Reference Books 1. New Delhi (1985) 9. spoilage factors of fish. London (1977) 4. F. USA (1971) 7. Unit V : Fish and other Marine Products Processing Commercially important marine products from India. West Port. merits and demerits. G. packaging and storage. in pack freezing.Hui and Others: Meat Science and Applications Marcel Dekkar Inc. New Jersey. T. Changes storage with RSW. Whole egg powder. Individual quick freezing. refrigerated storage of poultry meat. Albert Levie: Meat Hand Book (4 Edition) AVI Publishing Inc.W.Govindan: Fish Processing Technology Oxford and IBH Publishers. Lawson: Processing of Aquatic Food Products John Wiley and Sons Publishers. USA (1976) 6. Design of refrigerated and insulated trucks. use of Refrigerated Sea Water (RSW) for preservation. field refrigeration and icing practice.H. New York (1985) FP209 PLANTATION PRODUCTS AND SPICES TECHNOLOGY Marks (40+60) n u r a y i n U s r e v y it Credit : 4:0:0 Unit I : Chemistry and Technology of Coffee Coffee – Occurrence – chemical constituents – harvesting – fermentation of coffee beans – changes taking place during fermentation – drying – roasting – Process flow sheet for the School of Biotechnology 47 . Mountney: Poultry Products Technology AVI Publishing Inc. bleeding. John Kerry and David Ledwood: Meat Processing Woodhead Publishing Limited (CRC Press 2002) th 2. Y. Canning operations. Westport. microbiology of poultry meat. relative merits and demerits. Basic biochemistry. Joseph Kerry.Unit IV : Processing of Poultry Products : Poultry industry in India. B..Weiss: Poultry Processing Noyes Data Corporation. Salting and drying of fish. Freeze preservation. filling and grazing. measuring the yields and quality characteristics of poultry products. fish oil and other by products. freezing of prawn and shrimp. spoilage factors.

D. Estimating the Shelf life requirement of food products for packaging. New Delhi 2004. New York. Aspen Publication. National Institute of Industrial Research (NIIR) Board. 3. mechanical forces and biological factors on quality of food.manufacture of coffee powder – Instant coffee.S. fenugreek. 2. and Kadam S.Quality control Unit V : Chemistry and Technology of Minor Spices Cumin. effect of light. 1998. Salunkhe. Temperature. a K Credit : 4:0:0 Unit I : Introduction to Food Packaging: Protection of Food products as major role of food packaging: Functions of packaging.Quality grading of coffee Unit II : Tea – Chemistry and Technology Occurrence – chemistry of constituents – harvesting – types of tea – green. Ed. ginger and turmeric – Oleoresins and essential oils – Method of manufacture – Chemistry of the volatiles –Enzymatic synthesis of flavour identicals . Oxygen.K. USA. Clove and Vanilla . Handbook on Spices. III Edition. oolong and ctc – Chemistry and technology of CTC tea – Manufacturing process – Green tea manufacture – Instant tea manufacture – Grading of tea Unit III : Chemistry and Technology of Cocoa and Cocoa Products Occurrence – Chemistry of the cocoa bean – changes taking place during fermentation of cocoa bean – Processing of cocoa bean – cocoa powder – cocoa liquor manufacture Chocolates – Types – Chemistry and technology of chocolate manufacture – Quality control of chocolates Unit IV : Chemistry and Technology of Major Spices Pepper.Oleoresins and essential oils – Method of manufacture – Chemistry of the volatiles – Quality control Present trends in synthesis of volatiles – micro-organisms. Mechanical strength (Tension. Minifie Bernard W. technology – Chicory chemistry .. Moisture. accelerated storage studies: Vacuum and Inert Gas Packaging: Tests on packaging materials. Asia Pacific Business Press Inc. Chocolate. analysis of storage requirement.. notch and tearing strengths). plant suspension cultures Text Books / Reference books 1. Coriander. Hand book of Vegetable Science and Technology. Cardamom. Cocoa and Confectionery Technology. Effect of environmental factors on food quality and shelf life. Need for protective packaging. Cinnamon. General Approach. Gas and water vapour transmission rates n u r a y i n U s r e v Marks (40+60) y it FP210 FOOD PACKAGING TECHNOLOGY School of Biotechnology 48 . Marcel Dekker. Garlic. 1999.

D. Polypropylene (PP). USA (1993) 2. Shirly V. Bottle and jar closures. applications: Laminated Paper board Cartons. Lap Seals and Fin seals –Differences and advantages.Unit II : Metal Cans as Packaging Merits and demerits. Filling and sealing of Flexible plastic containers. advantages and disadvantages – Polyethylene (LDPE and HDPE). terminology. specialty of Open top sanitary cans (CANS). Polyvinyl chloride. New York 6. Basics of Canning operations. UK (1986) 4. Extrusion Extrusion Blow moulding etc. R. Relative merits and demerits.Principles and Practice Marcel Dekker Inc. H Graves and V. Tin cans and Aluminum cans. applications. Moisture control. Gordon L. Three piece cans and Two piece cans.David.Carlson: Aseptic Processing and Packaging of Foods: CRC Press. lug caps. Glass jars and Bottles in food packaging.R. relative merits and demerits.Vangrade and Morgy Woodburn: Food Preservation and Safety Surabhi Publications. Robertson: Food Packaging. Twist off lid and ROPP caps. Description and applications. J. Ixan and Saran). Seal types-Bead seals. Unit V : Interaction of Food Material with Packaging Material Active packaging. advantages and problems. Filling (Volumetric and Gravimetric) and Sealing of pouches. Copolymers their applications. Bar codes. Donald Downing: Complete Course in Canning (3 Volumes) CTI Publications inc. (Editor): Food Packaging and Preservation Elsevier Applied Science Publications Essex.. Unit IV : Filling and Sealing Operations for various types of packages. Lacquers and their use.Diofan. Can double seam. Can closures. USA (1996) 3. Design features and applications. Jaipur India 5. Hot wire sealing. Co-extruded films and Laminates Their applications. Text/Reference Books 1. Form fill Seal equipment: Printing on packages. different types of caps and liners used. Fibre Board and Corrugated Card Board packaging and their applications. Aerosol Cans. Injection moulding. hot bar sealing and impulse sealing – differences and relative advantages. CO2 and Oxygen scavenging. Mathlouthi M. Pouch form fill seal machines: Rigid and Semi rigid plastic packaging –Fabrication methods in brief –Thermo forming. Cellulose. Nutrition labeling and legislative requirements. Metal caps for bottles and jars – Crown corks. R. can seam formation and defects. Poyvinylidene Chloride (PVDC . Unit III : Flexible Films Packaging Relative merits and demerits. Sterilization of bottles. Blow moulding. Polyesters. Metallic can types employed. Closing and sealing of Rigid plastic containers. NIIR Board: Food Packaging Technology Handbook National Institute of Industrial Research. Plastics used and their Specific applications. New Delhi (2004) a K n u r a y i n U s r e v y it School of Biotechnology 49 . Formation of Films and pouches. Modified atmosphere packaging – principles.

sheeting. Sifting and purifying. Dividing. UK.Baver: Plastic Packaging Materials for Food Wiely VCH. O. Sifters. Moisture tests. GmbH. s and L. Destoners. Cleaning. Kalman Peleg: Produce Handling. CRC Press USA (2000) 12. Unit II : Outline of the Wheat Milling Process Wheat milling Flow sheet. Packaging and Distribution AVI Publishing Company. R. (1985) 11. a K n u r a y i n U s r e v y it Marks (40+60) School of Biotechnology 50 . Grain hardness testing. By products of wheat mill. Unit III : Bakery Equipment and Engineering Bulk handling of ingredients.Parry: Principles and Applications of Modified Atmosphere Packaging of foods: Blackey Academic and Professional.Rooney: Active Food Packaging : Blackey Academic and Professional. London (1995) 17. Wheat mill yields Energy requirement for different operations and its conservation. Amylograph. R. M. J Ralph Blanch Field (Ed. Aurand: Laboratory Manual for Food chemistry: AVI Publishing Company Inc. Fermentation enclosures and brew equipment. fluted rolls and plane rollers and their applications. Frank A. and laminating. Forming and molding bread-like products. Weighing and metering equipment. USA (1989) 15.T.G. Maida and other products.Taub. Paul Singh: Food and Storage Stability CRC Press. David Kilcast & Persis Subramaniam: The Stability and Shelf Life of Food Woodhead Publishing Limited. CRC Press (2000) 14. London. extraction rates and control of extraction. Visco graph. Pneumatic conveying of products. Irwin A. Extraction.Crossby: Food Packaging Materials Applied Science Publishers. Aaron L. plan sifters. milling for Atta.T. Details of gradual reduction and purifying system. N. Brody: Controlled/Modified Atmosphere/Vacuum Packaging of Foods Food And Nutrition Pres. USA (1997) 13.L.Paine: A Hand Book of Food Packaging Leonard Hill Publications (Blackie and sons) (1983) 8. Germany (2000) 9. explanation of steps in milling operations of milling. Forming cookies.Pirenger and A.L. USA ( 1977) FP211 MILLING AND BAKERY TECHNOLOGY Credit : 4:0:0 Unit I : Fundamentals of Market Analysis for Wheat and Wheat Products: Laboratory testing of Wheat grain Quality. Semolina of different particle size. London (1993) 16. Wet Washing. crackers and other products.): Food Labelling Woodhead Publishing Inc. USA (1985) 10.W.Westport. Grits and Bran.7. Inc. Extruded macaroni products from Wheat semolina. Paine and Heather Y. Roller milling – Break rolls. Testing of wheat flour for bread making and Chapathi making. Dough mixing and mixers. Ferinograph and other tests and their significance in bread manufacture. rounding.

emulsifiers and antioxidants. 8. Sanitation and safety. and nuts. Yeast laminated products. E B Bennion. short doughs. A Bent. Dough retarding and freezing – Specialty fermented goods. Spices. G S T Bamford: The technology of cake making. and colors Unit V : Biscuits and Cake Manufacture Biscuits – Ingredients and flour specification . Stanley P Cauvain. A. Ingredients from milk and eggs. Accelerated processing. and Slicers. Crackers. 1997 9. Blackie Academic and Professional. Samuel A.Kent. Ingredients from other grains. Matz: Equipment for Bakers Pan Tech International Publication (1988) 6.. Preservation methods. Sixth edition. Applicators for adjuncts.Part-baked breads. semi-sweet. Leaveners and yeast foods. Linda S Young: Technology of Breadmaking: Second Edition Aspen publication. pan handling equipment. UK. 1999 7. Shortenings. Y. N. Packaging materials and equipment. Materials of Baking .Types of biscuit doughs – Developed doughs. enzyme modified doughs and batters.R. Unit IV : Baking Technology Bread manufacturing process – Straight dough fermentation.Ingredients from wheat. Pans.Evers Technology of Cereals: Peregaman Press (Elsevier Publishers) (1994) 4. England 1998. NY (1987) 3. Samuel A. Fruits. Cauvein: Bread Making – Improving Quality Woodhead Publishing(CRC Press 2003) 10.Ovens and baking. vegetables. Manley: Technology of Biscuits. Chorley wood bread process. USA 1963 2. Sponge and dough.D.L. Matz : Bakery Technology and Engineering (3rd Edition Published by Chapman & Hall (1992) 5. Computerization in plant and laboratory. Pomeranz Modern Cereal science and Technology: MVCH Publications. and Cookies Ellis Horwood Ltd (1983) n u r a y i n U s r e v y it School of Biotechnology 51 . by Woodhead Publishing Ltd. Stanley P.importance of the consistency of the dough Cake manufacture – Flour specification – ingredients – manufacturing process – types of chemically aerated goods a K Text/Reference Books 1. Association of Operative Millers Cereal Millers Hand Book: Burgess Publishing company. Duncan J. Duncan Manley: Biscuit Doughs Manual 2. Fryers and frying. Sweeteners and malt syrup. flavors. Water and salt.

FP212 ANALYSIS OF FOOD CONSTITUENTS – 1 Credit : 0:0:2 Marks (50+50) 12 experiments will be notified by the HOD from time to time FP213 PROCESSING OF FOOD – 1 Credit : 0:0:2 12 experiments will be notified by the HOD from time to time FP214 ANALYSIS OF FOOD CONSTITUENTS – 2 Credit : 0:0:2 12 experiments will be notified by the HOD from time to time FP215 FOOD ADDITIVES LAB Credit : 0:0:2 12 experiments will be notified by the HOD from time to time FP216 ANALYSIS OF FOOD COMMODITIES – 1 Credit : 0:0:2 a K n u r 12 experiments will be notified by the HOD from time to time a y i n U s r e v Marks (50+50) Marks (50+50) Marks (50+50) Marks (50+50) y it School of Biotechnology 52 .

SCHOOL OF BIOTECHNOLOGY a K n u r a y i n U s r e v y it School of Biotechnology AN-135 .

energy rich compounds. Unit – III : Intermediary Metabolism And Bioenergetics Overview of intermediary metabolism: Interconnections and regulation of metabolic pathways. Lehninger. pentose phosphate pathway. pyrimidines. Lys. and Cox. polynucleotides. Carbohydrate Metabolism: Glycolysis. glycolipids and steroids. M. 2000. D. peptides. phospholipids. Thr. glycogenesis and glycogenolysis. New York. TCA gluconeogenesis. nucleotides. Inborn errors of their metabolism. ribonucleic acids.Leu. nucleoprotein complexes and structure-function relationship in nucleic acids. sphingolipids. Met. References: 1. Structure and properties of purines. M. deoxy ribonucleic acids. oxidative phosphorylation and triose phosphate cycle. (2000).. Bioenergetics: redox biochemistry. Unit – II : Structure and Properties of Proteins and Nucleic Acids Structure and properties of amino acids. nucleosides. . a K Unit-V : Metabolism Of Proteins And Nucleic Acids Biodegradation of proteins and nucleic acids. modified amino acids. WH Freeman & Co. Unit – IV : Metabolism Of Carbohydrates and Lipids Lipid Metabolism: Biosynthesis and biodegradation of fatty acids and cholesterol. Inborn errors of metabolism. Nelson. Lubert Stryer. conjugated proteins and structure-function relationship in proteins. Biosynthesis and biodegradation of important amino acids. Tyr. purines and pyrimidines. L. di. Phe.ADDITIONAL SUBJECTS Code BC209 BC210 BC211 BC212 BC301 BC302 BC303 BC304 Subject Name Biochemistry Bioorganic chemistry Instrumental Methods of Analysis Food Biochemistry & Nutrition Cell Biology and Advanced Biochemistry Cell Biology and Advanced Biochemistry Lab Food Biochemistry and Nutrition Food Biochemistry Lab Credit 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 0:0:2 3:1:0 0:0:2 BC209 BIOCHEMISTRY Credit : 4:0:0 Unit . Principles of Biochemistry Third Edition (Freeman Publishers). Biochemistry. Trp. Text book: 1. School of Biotechnology AN-136 n u r a y i n U s r e v y it Marks (40+60) cycle.I : Structure and Properties of Carbohydrates and Lipids Structure and properties of mono.. Structure and properties of fatty acids. Glutamate and Cys-Urea Cycle. A. respiratory chain. proteins. 4th Edition. complex carbohydratesStructural and functional relationships. oligo and polysaccharides. L.

Carboxy peptidase. Unit – III Case studies of enzyme structure and mechanism Acid base and covalent catalysis. Organic chemistry by paula yurkaris Bruice. Stereochemistry of amino acids. Rodwell. peptide synthesis. Mayes. Prentice Hall International. Specificity of enzymes. 3rd edition. its dynamics.. types. stability of proteins and molecular chaperones. 6 Edition.K. E. New Delhi. lysozyme. Biochemistry by Mathew . Optical isomers.. V. Unit – II Chemistry of enzymes Structure of atom. S notation. Trevor Palmer. chemical bonding and its characteristics. . Voet and Voet. 2. Athern . Mechanisms of E1 and E2 reactions.Principles..H. 3. proteases. Circular dichorism . Biochemistry. Text. nd BC210 BIOORGANIC CHEMISTRY Credit : 4:0:0 Marks ( 40+60) Unit – I Concepts in organic chemistry Stereochemistry. 2005. free radicals systems.Mechanism of SN1 and SN2 reactions.an overview. optical activity and optical rotation-polarimeter. Concept of Free energy and its importance in biology. NAD. Murray..K. Harper’s Biochemistry. a K n u r a y i n U s r e v y it School of Biotechnology AN-137 . 2. References: th 1. Jain & Sunjay Jain. B. Protein folding. Enantiomers.R. John Wiley & Sons Inc. 2002. New Delhi. East West Press P Ltd. 2 Edition.Dugas . Chemical bonding involved in protein structure. 3. 1999. Fundamental of Biochemistry by J. Unit IV: Hydrolysis in chemical synthesis Ester hydrolysis. Amide hydrolysis. NMR.W. Van Holde. (2000). Application of enzymes in industry and medicine. Pearson P Ltd. structure and mechanism of FAD THF coenzymes. Structure and mechanism of pyruvate dehydrogenase. 1995. UNIT V : Protein folding kinetics and folding pathways Structure of proteins.. Basic methods of protein folding – two state kineticsmultistate kinetics-in protein folding. 2000. ribonucleases. Granner. New Delhi. R.2. Dependent oxidation and reduction reactions. Z Isomerism. Pearson Publishers Ltd.A. P. Bioorganic chemistry. Chemistry of active oxygen species.L. 2004. Text books: 1. Enzymes. Springer Verlag Publishers. components and applications in Biology.

thin layer.principles.Lambert’s law. Isoelectric focussing. starch. D. buffering systems of blood. calibration of instrumental methods. Ewing GW. 2000. Metabolic regulation.thermo gravimetric. Scintillation counter. Introduction to Chemical Analysis. signal to noise ratio. ion-exchange. 2.Basic theory. isotachophoresis. TCA cycle. gas and HPLC. supporting materials-paper. UNIT III Chromatography and Electrophoresis Chromatography.BC211 INSTRUMENTAL METHODS OF ANALYSIS Credit : 4:0:0 Marks (40+60) UNIT I Buffer Solutions Definitions. types . CBS Publishers & Distributors. Flame photometer and Spectrophotometer: types– UV – visible – IR – Raman spectroscopy. construction and working of Differential Thermal Analysis (DTA) and Differential Scanning Calorimeter (DSC) Text Book 1. 2. Instrumental Methods of Analysis. UNIT V Thermo Analytical Techniques Theory of thermal analysis. determination of pH using H2 electrode and glass electrode. radioactive techniques-RIA. agarose. description and application of Colorimeter. polyacrylamide. Braun. derivation of Henderson-Hasselbalch equation and its application. 1992. Pentose phosphate shunt. Willard and Merrit. interconnection of pathways.paper. energy rich compounds School of Biotechnology AN-138 . 1987. adsorption. types – disc. UNIT II Colorimeter. GM counter. Flourimeter. Flourimeter. Reference Books: 1.glycolysis. Skoog. Flame Photometer and Spectrophotometer Beer . H. Autoradiography. affinity. VI Edition. Principle. urea cycle. gel filtration. Electrophoresis – principles. McGraw Hill. Embden Meyerhof Pathway (EMP). Bioenergetics: Respiratory chain ATP cycle. Instrumental methods of Chemical Analysis. preparations. a K n u r a y i n U s r e v y it BC212 FOOD BIOCHEMISTRY AND NUTRITION Marks: 40 + 60 Credit : 4:0:0 Unit I Electron transport chain . radioactive decay and their types. Instrumental Methods and Analysis. McGraw Hill Book Company. UNIT IV Radio active techniques Radioactive isotopes. 1989. Applications in Medicine & Diagnosis. gluconeogenesis. immuno-electrophoresis.

Blackwell Publishing 3. Ageing –Theories of ageing – Nutrition and ageing – Cancer and its prevention . Lehninger A.Nutrition and disorders associated with organs such as liver and kidney . Biochemistry.L. protein and fat metabolisms . Second Edition. alzheimer’s disease Text / Reference Books: 1. Voet D. Biosynthesis and degradation of purines. heterocyclic. diabetes. essential amino acids .Biosyntheses and degradation of amino acids (one example each for sulphur containing. Biochemistry. proteins and fats – determination of energy value – balanced diet – Recommended dietary intake – Acceptable dietary intake – Protein efficiency ratio – Net protein utilisation and their determinations – Malnutrition and its problems – Nutrient supplementation – fortification . aliphatic. 1997 Reference Books 1. John Wiley and Sons. CBS Publications. aromatic.Age-related metabolic disorders – Nutrition in the treatment of age-related disorders like hypertension. Fouth edition. M. Food and Nutrition. Unit III Basic concept of nutrition – Importance of nutrition and dietetics . enzyme inhibitors.Functional foods. and cholesterol . Edited by Ronald Ross Watson a K n u r a y i n U s r e v y it BC301 CELL BIOLOGY AND ADVANCED BIOCHEMISTRY Marks: (40+60) Credit: 4:0:0 UNIT I Structure. lectins. 2. peptides and proteins.Micellesdifferent types of proteins & extra cellular matrix. Cox. pyrimidines and nucleic acids. Function and Transport Mechanisms of the Cell Structure and function of Eukaryotic and prokaryotic cells. carbohydrates in food and dietary fibre Unit IV Inborn errors of carbohydrate.Assessment of nutritional status – energy value of carbohydrates. phytates Unit V Nutrition for specialized purposes – Pediatric nutrition – geriatric nutrition – Sports nutrition – Nutrition during pregnancy .Unit II Biosyntheses and degradation of fatty acids.L. Nelson D. 1994. phytoallexins. Edited by Martin Eastwood. Voet G. 3.M. Wahlquist M L. 1994. Transport in eukaryotic cells-Passive & School of Biotechnology AN-139 .it’s membrane.. Principles of Human nutrition – Second Edition.Digestibility.Nutritional labeling and its importance . Functional foods and Nutraceuticals in Cancer Prevention. 2.Naturally occurring anti-nutritional factors – Cyanogens. Principles of Biochemistry. basic and acidic amino acids).Effect of processing on protein quality. 1993. Stryer L.

Donald Voet and Judith G. structure. Victor W.Daryl K. enzyme inhibition. Cell cycle –it’s control molecules & mechanism-apoptosis-oncogenes. trans-membrane potential coupled ATP generation. Rodwell. P.signal amplification. non – competitive and mixed inhibitors. UNIT II Cell-Cell Signalling and Apoptosis Receptors . energy rich compounds. Darnell J. pentose phosphate pathway. N. Budha Academic Publishers Pv. Md Murray. properties and function of enzymes. lipids and vitamins.G.Biochemistry – Volume 1 Biomolecules. UNIT IV Enzymes Enzymes-action. UNIT V Metabolic Pathways and Bioenergetics Energy yielding biochemical pathways: Biodegradation of amino acids. M. specificity. Transport into prokaryotic cells-group transportation. Glycolysis. proteins and nucleic acids..active transport. purines and pyrimidines. UNIT III Introduction to Biomolecules Biomolecules: Occurrence. classification. Structural and functional relationships in complex carbohydrates. Voet . W. Different models of signal transduction-signalling by protein kinases-serine/threonine kinase –tyrosine kinase-MAP kinases. Robert K. Mayes. oxidative phosphorylation and triose phosphate cycle.Peter A. A. M. classification. Entry of virus and toxins into cells. Robert K. Granner. Lehninger. kinetics of competitive. properties and functions of carbohydrates. D. Prasad (2003). fatty acids. Molecular Cell Biology.Appleton and Lange.O. Reference Books 1. proteins. Baltimore D. Fourth edition. Mechanisms of Enzyme Action. A Text Book of Biotechnology by B. Harper’s Biochemistry – (2000). Murray 2. 3.H.. application of enzymes. Principles of Biochemistry Third Edition (Freeman Publishers).Ltd. endocytosis and exocytosis. respiratory chain. structure. (2000). and Cox. Bioenergetics: Redox biochemistry. 2004.Nepal a K n u r a y i n U s r e v y it BC302 CELL BIOLOGY AND ADVANCED BIOCHEMISTRY LAB Marks: (50+50) 12 experiments will be notified by the HOD from time to time Credit: 0:0:2 School of Biotechnology AN-140 .Menton equation. Enzyme kinetics: Michales. allosterism. New York. stabilization of proteins and nucleic acids. cholesterol. TCA cycle. & Metabolism. Nelson.Freeman (2002). Lodish H.endocrine models of action. Text Book 1. L.Box 20195 Kathmandu. L. 2.

CBS Publications. Voet G. gluconeogenesis. 2.Nutritional labeling and its importance .BC303 FOOD BIOCHEMISTRY AND NUTRITION Credit : 3:1:0 Marks: 40 + 60 Unit I: Electron transport chain . Unit III: Basic concept of nutrition – Importance of nutrition and dietetics . Metabolic regulation. Stryer L. Wahlquist M L. Nelson D. essential amino acids .Functional foods Ageing –Theories of ageing – Nutrition and ageing – Cancer and its prevention . Biochemistry. Edited by Martin Eastwood. phytates Unit V: Nutrition for specialized purposes – Pediatric nutrition – geriatric nutrition – Sports nutrition – Nutrition during pregnancy . Biochemistry.Biosyntheses and degradation of amino acids (one example each for sulphur containing.Age-related metabolic disorders – Nutrition in the treatment of age-related disorders like hypertension.glycolysis. heterocyclic. Edited by Ronald Ross Watson School of Biotechnology AN-141 n u r a y i n U s r e v y it . Embden Meyerhof Pathway (EMP). 1997 Reference Books: 4. proteins and fats – determination of energy value – balanced diet – Recommended dietary intake – Acceptable dietary intake – Protein efficiency ratio – Net protein utilisation and their determinations – Malnutrition and its problems – Nutrient supplementation – fortification . 1994.. urea cycle. lectins.Effect of processing on protein quality. energy rich compounds Unit II: Biosyntheses and degradation of fatty acids.Assessment of nutritional status – energy value of carbohydrates. Biosynthesis and degradation of purines. diabetes. Lehninger A. Functional foods and Nutraceuticals in Cancer Prevention. Principles of Human nutrition – Second Edition.Naturally occurring anti-nutritional factors – Cyanogens. John Wiley and Sons. enzyme inhibitors. aliphatic.M.Nutrition and disorders associated with organs such as liver and kidney . Food and Nutrition. phytoallexins.Digestibility. M. 1994. 5. Cox. pyrimidines and nucleic acids. Voet D. Fouth edition. basic and acidic amino acids). Principles of Biochemistry. Blackwell Publishing 6. TCA cycle. Second Edition.L. aromatic. Pentose phosphate shunt. interconnection of pathways. and cholesterol . carbohydrates in food and dietary fibre Unit IV: Inborn errors of carbohydrate. Bioenergetics: Respiratory chain ATP cycle.L. alzheimer’s disease a K Text Books: 1. protein and fat metabolisms . peptides and proteins. 3. 1993.

BC304 FOOD BIOCHEMISTRY LAB Credit: 0:0:2 Marks: (50+50) 12 experiments will be notified by the HOD from time to time a K n u r a y i n U s r e v y it School of Biotechnology AN-142 .

Fed. Components of a microbial bioprocess.ADDITIONAL SUBJECTS Code MB301 MB302 MB303 MB304 MB305 Subject Name Microbial Biotechnology Microbial Biotechnology Lab Molecular Biology and Genetics Food Microbiology Food Microbiology Lab Credit 4:0:0 0:0:2 4:0:0 3:1:0 0:0:2 MB301 MICROBIAL BIOTECHNOLOGY Credit: 4:0:0 Unit. Compartmental & metabolic models. Azolla.II Industrial and Environmental Microbiology Primary metabolites. simple unstructured kinetic models for microbial growth. Monod and Leudeking – Piret models: Monod’s model including the effects of inhibition. cyanobacteria. chemically & genetically structured models. Structural organization and multiplication of bacteria. biofertilizers: rhizobia. Azospirillum. series of CSTRs.I Introduction to Microbiology Basic of microbial existence: history of microbiology. fluidized bed reactors& membrane bioreactors. determination of kinetic parameters by batch. Azotobacter. growth curve and different methods to quantitate bacterial growth.Anabaena symbiosis. The Ideal Batch reactor.DNA products. classification and nomenclature of microorganism. Role of maintenance and endogenous metabolism in substrate utilization and growth. Unit. production of penicillin and alcohol. production of cyanobacterial inocula.Batch and Plug flow Reactors. secondary metabolites and their applications. fed batch and continuous culture and analysis of chemo state performance.V Analysis of ideal bioreactors Chronological development of industrial microbial biotechnology Range of products. UNIT III Microbial Growth.IV Structured Models for Growth and Product Formation Introduction to structured models for growth and product formation. Nutritional requirements of bacteria and different media used for bacterial culture. Unit. Continuous Stirred Tank Reactor (CSTR). substrate utilization and product formation. product formation kinetics: Gaden’s and Deindoerfer’s classifications. viruses. a K Unit. School of Biotechnology AN-143 n u r a y i n U s r e v y it Marks: (40+60) . aerobic and anaerobic bacteria. Maintenance and Product Formation Phases of cell growth in batch cultures. Kinetics. algae and fungi. bioremediation. Considerations for the production of r.growth associated product formation kinetics. biopesticides and biosensors. production of Azolla. growth associated and non . Biopreservation of food. packed bed reactors. kinetics of growth & product formation by filamentous organisms. Mycotoxin. mycorrhizae (AMFungi).

Principles of Cell Energetics: BIOTOL series. New Delhi. 2 edition. 2. WHITAKER. RNA interference in medical applications Unit IV: Translation and Regulation of Gene Expression Elucidation of genetic code. ribozyme. Reference Books: 1. nd 2. Atkinson & F. 2001.Kinetics & Reactors by A Moser. 1988. DNA mutation and repair.Heinemann. McGrawHill (1986). post-transcriptional modification . Unit III: Transcription Prokaryotic and eukaryotic transcription. post-translational modifications. Stockton Press ( 1991). Principles of fermentation technology by P F STANBURY.RNA splicing. replication of telomeres in eukaryotes. classical experiments – Hershey and chase. Hall. Avery McLeod & McCarty. Mavituna. Biochemical Engineering and Biotechnology Handbook by B. India. Mendelian genetics. Butterworth. Chan ECS And Krein NR. 4.Lac and trp operons. Microbiology. linkage. 3. crossing over. 2 ed. Pelczar MJ. features of promoters and enhancers. Lytic and lysogeny Unit II: DNA Replication Replication in prokaryotes and eukaryotes. 1993. 1999. 3. transduction and transformation. Bacterial conjugation. recombinations. Tata McGraw Hill Edition. inhibitors. School of Biotechnology AN-144 a K n u r a y i n U s r e v y it Marks: (50+50) Marks (40+60) . Mutations – chemical and physical mutagens – DNA damage and repair mechanisms. process of translation in prokaryotes and eukaryotes. D-loop and rolling circle mode of replication. replication of linear viral DNA. Bioprocess Technology. Regulation at various stages of gene expression in eukaryotes and prokaryotes . Rev&Expand edition. transcription factors. RNA editing. S. MB302 MICROBIAL BIOTECHNOLOGY LAB Credit: 0:0:2 12 experiments will be notified by the HOD from time to time MB303 MOLECULAR BIOLOGY AND GENETICS Credit : 4:0:0 Unit I: Classical Genetics Different forms of DNA. Biochemical Engineering fundamentals by J E Bailey and D F Ollis.Text Books: 1. inhibitors of protein synthesis. Organization of eukaryotic chromosome – cot value. A. suppressor mutation. Butterworth-Heinemann. Springer. 2nd Ed.

Irradiation – Brief account of microwave. Vegetables and Vegetable products . 1987 MB304 FOOD MICROBIOLOGY Credit : 3:1:0 Unit I: Introduction and Methodology Micro organisms associated with foods: Bacteria – Beneficial and Contaminants Molds. Principles of Genetics. electrical methods. Immunological methods – Serotyping. Narosa Publ. Processed meat and sea foods. Agarose gel electrophoresis in DNA separation. Unit II: Conventional methods of preservation Thermal mode of preservation – Pasteurisation and appertization – determination of D and z values – spoilage of canned foods – aseptic packaging .. Intestinal viruses. isolation and enumeration of microorganisms . Oxford University Press. Nuts and Oilseeds. Definitions.Recent developments – Direct Epifluorescent Techniques. Eggs. Dye-reduction tests. Gardner / Simmons / Snustad. Thermostable nuclease test.Traditional vegetable fermentation – Sauerkraut. Viruses. and DNA/RNA methodology. UV and ionizing radiation . Lantibiotics a K n u r a y i n U s r e v y it Marks: 40 + 60 School of Biotechnology AN-145 . Gene VII. ATP determination – Rapid methods for the detection of specific organisms and toxins – Limulus lysate for endotoxins. Dairy products .Unit V: Recombinant DNA technology Cloning – vectors – plasmids – cosmids – restriction enzymes – steps in cloning – genomic and cDNA library – gene amplification using PCR.Factors affecting their growth – Recent developments . Eighth Edition.antimicrobial barriers and constituents. 2000 2. Methods of screening. Roberts JW. Fruits and Fruit products. Steitz JA. Molecular Biology of the Gene. Benjamin Lewin. Molecular Biology. Pulses. 1999 2. Hydrophobic grid membrane filter. David Friefelder.Dairy fermentations – Importance of Lactic acid bacteria. John Wiley Reference books 1. Bacterial viruses and Fungal Viruses .Low-temperature storage Non-thermal methods of preservation High pressure processing – Pascalisation . House. Weiner AM. Watson JD. Natural food preservatives Microbiological quality control and HACCP Unit III: Microbiology of Food commodities of plant origin Cereals. Hopkins WH. Advances in genetic engineering methods and applications.Use of chemical preservatives. Lactic acid fermentation – Recent developments in cereal-based lactic acid fermentations Unit IV: Microbiology of Food commodities of animal / marine origin Fresh meat and poultry. Text Books 1.

M. USA. Moss.R. Bibek Ray: Fundamental Food Microbiology. Brucella. Third reprint 2004 References: 1. 2005.. Modern Food Microbiology. Gastroenteritis viruses. Food Microbiology Second Edition Panima Publishing corporation. Camphylobacter. W. 3. (Third Edition) CRC Press December 2003. Adams and M. New Delhi. MB305 FOOD MICROBIOLOGY LAB Credit : 0:0:2 12 experiments will be notified by the HOD from time to time a K n u r a y i n U s r e v y it Marks: 50 + 50 School of Biotechnology AN-146 . Hepatatis A and E. Salmonella.O. Seventh Edition. Bacillus cereus. Escherichia coli. 2. Staphylococcus aureus.J. Spongiform encephaolpathies Text Books 1. Scombrotic fish poisoning. Gustavo F Gutierrez-Lopez. and Golden D. Loessner M.Frazier: Food Microbiology (II edition or later) Mcgraw Hill Book Company. M.. New York (1968) 2. Gustavo V Barbosa-Canovas Food Science and Food Biotechnology: CRC Press 2003.A. Jay J.C. Springer.Unit V : Food borne pathogens Aeromonas hydrophila. Clostridium botulinum.

Zeroth law of thermodynamics. Convection and Radiation. 2. UNIT – II: Introduction to thermodynamics Thermodynamic system and Control volume. Basic concepts multiphase flow.laminar. dryness fraction.2005. UNIT – III: Heat Transfer – Fundamentals: Modes of heat transfer –Conduction. Ltd. Yunus A. LMTD. S. thermodynamic Properties. overall heat transfer coefficients. Robert H. Text Book: 1. pressure drops in pipes (Simple problems in incompressible fluids). Thermodynamic equilibrium. Pressure. Second law of thermodynamics. SFEE.Cengel.New Delhi. NTU. turbulent.Turner. School of Biotechnology AN-147 n u r a y i n U s r e v y it Marks (40+60) . mass and enthalpy balances. Steady state conduction. Shell and tube condensers.IV: Heat Transfer with phase change: Boiling and condensation. Temperature. Equation of state-ideal gas. A course in heat and mass transfer-Dhanpat rai & Co. UNIT – V: Heat Exchangers: Equipments-Types. Tata McGraw Hill. Flow boiling. Pool boiling. capillary effect.. evaporators. surface tension. Bernoulli equation.ADDITIONAL SUBJECTS Code CL211 CL212 CL213 CL214 CL215 CL216 CL301 CL302 Subject Name Fundamentals of Thermal Fluid Sciences Unit Operations of Chemical Engineering Process Economics and Plant Design for Food Engineers Biochemical Engineering Fundamentals of Thermal Fluid Sciences for Food Engineers Economics and Food Industry Management Biochemical Engineering Biochemical Engineering Lab Credit 4:0:0 4:0:0 4:0:0 3:1:0 4:0:0 3:1:0 4:0:0 0:0:2 CL211 FUNDAMENTALS OF THERMAL FLUID SCIENCES Credit : 4:0:0 UNIT – I: Fluid Mechanics: Fluids-Properties. concept of work and heat transfer. Analysis of Heat Exchangers.” Fundamentals of Thermal Fluid Science”. thermodynamic process. a K UNIT . Introduction to convection -Forced and natural. (Simple problems in SFEE). Properties of steam. Vapour pressure. Arora. (Simple problems). Introduction to Radiative heat transfer (Derivation not required-Simple problems). Types of fluids.single and multiple effects. latent heat.Domkundwar. First law of thermodynamics –closed and open system. Fluid flow. (Simple problems). sensible heat.

circulation. Mechanics of particle motion. Geankoplis C. a K Text Book: 1. Smith J. 2. Minimum fluidization velocity UNIT – II Filtration Constant pressure.Vol 2 Particle Technology and Separation Processes.. material balances without and with chemical reactions. 3. settling and sedimentation. unit operations. constant volume batch filtration.2002. Motion of spherical particle. continuous filtration.Coulson & Richardson's Chemical Engineering.1998. Coulson. 2002. UNIT – I Mixing and Agitation Agitation of liquids.J. environment. “A Text book of fluid Mechanics & Hydraulic Machines”. humidity calculations. Harker . 5th Edition. Fundamentals Of Heat And Mass Transfer. 1977. CRC Press School of Biotechnology AN-148 n u r a y i n U s r e v y it Marks (40+60) . Vora S.M. Richardson. 3..F. 2. Velocities and power for agitation. Transport Processes And Unit Operations.K. gas-liquid systems. Stoichiometry. CL212 UNIT OPERATIONS OF CHEMICAL ENGINEERING Credit : 4:0:0 UNIT – I Overview of Process Engineering Mass and energy conservation. integration. McCabe W. John Wiley. Harriot P. Butterworth & Heinemann . J. Tata McGraw-Hill. Laxmi Publications (1992).L. Bansal. 1993. 2. Albert Ebarz: Unit Operations in food Processing. steady and unsteady state. Friction in flow through beds of solids.P. 3rd Edition. 1993. numerical differentiation. gassolid suspensions.M. “A course in heat engines and thermodynamics”. et al. Reference Books: 1.Elsevier science Ltd. Reference Books: 1. Fifth Edition. process automation. recycle and by pass. Incropera F. Dhanpat Rai & Sons. Bhatt B. Kothandaraman. applied mathematics for experimental curve fitting. J. J.C.P. McGraw-Hill Inc. Terminal velocity. R.I. Prentice Hall India. Unit Operations In Chemical Engineering.. UNIT – V Flow Through Packed Columns Fluidisation-Drag force.H. UNIT – II Material Balances Overall and component balances. degrees of freedom. coefficents.C.3rd Edition. industrial filters..2002.

waste disposal. human resources.B. Optimization techniques – Linear and Dynamics programming.). Strategy of Process Engineering.g. instrumentation. process development. site characteristics. Reference Book 1. maintenance and repair. Food Process flow sheets – Hygienic food process design . raw materials. operating supplies. power and other utilities. Manufacturing costs – Direct production costs(including raw materials. Marcel Dekker Inc. 1987. community factors and other factors affecting investment and production costs. 1989. working capital investments. Maroulis Z. – Process Profitablity Application to a Food Processing plant e. Tomato processing Unit IV Plant overheads Administration.D. Wiley. land and utilities. availability of technology. payroll overheads. Optimization strategies. Peters and Timmerhaus. 2. installation costs (including equipments. 2003. piping. electrical installation and other utilities).CL213 PROCESS ECONOMICS AND PLANT DESIGN FOR FOOD ENGINEERS Credits : 4:0:0 Marks (40 + 60) Unit I Food Process Design Development Technical feasibility survey of Food Industry. interest rate of return. and Saravacos G. govt. Plant design and Economics for Chemical Engineers. building. Unit III Project evaluation and Cost Estimation Capital investments – fixed capital investments including land. Food Process Design. accounting for uncertainty and variations and future developments. human resources. safety and other auxiliary services. a K n u r a y i n U s r e v y it School of Biotechnology AN-149 . equipments. th McGraw Hill 4 Edition.equipment design and specifications – Computedaided process design – Principles of spread-sheet aided process design (Basic concepts only) Unit II General design Consideration Marketability of the product. equipments and utilities. Text Book 1. royalties. Rudd and Watson. etc. regualtions and other legal restrictions. warehouse and storage facilities etc Unit V Profitability Analysis Return on original investment..

William M Fogarty: Microbial Enzymes and Biochemistr. Micro Filtration membrane filtration and centrifugal separation techniques.: Biochemical Engineering. Clark. basics of Modelling and Scale-up. M. Braur: Bio Technology (Vol-2. Freeman Press. agitators.CL214 BIOCHEMICAL ENGINEERING Credits : 3:1:0 Marks (40 + 60) Unit I Fermentation Kinetics Fermentation mechanisms and kinetics: kinetic models of microbial growth and product formation Stoichiometry. Conducto-metric biosensors.S. immobilization and membranes. Sterilization of media and air. Biochemical product recovery and separation. Yields. Extraction.W. London (2004) 3. Blanch and D. 1985 3. H.E. Taylor and Francis Publications. Bailey and D. Basics of design of reactors. batch and continuous sterilize design. Fermenter types. Foil. School of Biotechnology AN-150 n u r a y i n U s r e v y it . Air lift Reactors and packed reactors. Kargi: Bioprocess Engineering – Basic Concepts. Marcel-Dekker. M. Direct Electron transfer biosensors and Optic biosensors. 1996 2. Organisms involved. 2nd Edition. J. 1986. Ollis: Biochemical Engineering Fundamentals. Modeling of batch and continuous Fermenter Unit II Bioreactor design Mixing phenomena in bioreactors. Bio process control and control instrumentation. 2nd Edition. a K Text Books 1. Unit III Down stream processing operations Solid -Liquid and Liquid-Liquid Separation processes. London (1983) 4. Fersht : Enzyme Structure and Mechanism. Biosensor development and diversification. Paddle and Helical agitators and characteristics. McGraw-Hill. El-Mansi and Charlie Bryce: Fermentation Microbiology and Biotechnology. tank design. Unit IV Industrial production of important products Production of Pectic Enzymes-sub merged fermentation and semisolid fermentation techniques: Industrial production of Glucose transforming enzymes. production. Fundamentals of Biochemical Engineering) VCH Publications (1980). Practical forms of Biosensors. transducer combinations.Shuler & F. Drying of products. H.L. 2. Microbial oil production and Bio pesticides. Prentice Hall (Second Edition 2002) Reference Books 1. Unit V Biosensors Definitions. Reaction rates. Applied Science Publishers. Industrial scale Production of Bakers’ yeast and Brewer’s yeast. Chromatographic techniques for separation. Stirred tank reactors. purification and immobilization of (a) Glucose isomerase and (b) Glucose Oxidase. sterilization equipment. specific applications of biosensors.F.

Evaluation of convection heat transfer coefficient. sensible heat. a K n u r a y i n U s r e v y it School of Biotechnology AN-151 .Cass: Bio Sensors – A Practical Approach. Oxford. Ghose: Bioprocess Computations in Biotechnology (Vol. Basic concepts multiphase flow. Steady heat conduction in simple geometries – Plane wall. V.E.Joshi and Ashok Pande: Bio Technology: Food Fermentations (Vol. A. thermodynamic Properties. Pitot tube-working principles only.G. (1990) CL215 FUNDAMENTALS OF THERMAL FLUID SCIENCES FOR FOOD ENGINEERS Credit: 3:1:0 Marks:40+60 UNIT I: Introduction to Fluid Mechanics: Fluids-Properties.1) Ellies Horwood Limited. Convection and Radiation. Bio Process Engineering: Wiley International Publications. Butterworth Heinemann limited. Rotameter. UNIT II: Introduction to thermodynamics Thermodynamic system and Control volume. Scott: Bio Sensors for Food Analysis. A. New Delhi (1999) 7. Bernoulli equation. surface tension.5. J. pressure drops in pipes. UNIT III: Heat Transfer – Conduction Modes of heat transfer –Conduction. Zeroth law of thermodynamics. Tarun K. Flow boiling. turbulent.James: Bio Process Technology: Modelling and Transport Phenomenon. latent heat. Vapour pressure. London (1990) 6. Pool boiling. Orificemeter.laminar. cylindrical wall without heat generation. Lyderson.(Simple calculations). concept of work and heat transfer. Types of fluids. (Simple problems). Oxford University Press. Nelson. Nancy A. First law of thermodynamics –closed and open system. Second law of thermodynamics. Basics of Radiation heat transfer. D’elia and Kim L. Bjorn K. turbulent flow over a flat plate. capillary effect.K. Fluid flow. critical radius of insulation Overall heat transfer coefficient (Derivation not required-Simple problems). New York (1994) 9. (Derivation not required-Simple problems). I and II) Educational Publications and Distributors. valves and bends.W. Thermodynamic equilibrium. (1992) 8. dryness fraction. Equation of state-ideal gas. heat transfer coefficient for Laminar flow in a tube with constant heat flux and constant wall temperature. UK (1998) 10. Unit IV: Heat Transfer – Convection and Radiation: Convection heat transfer – forced and natural. Transient heat conduction – Lumped capacitance method for bodies of infinite thermal conductivity Theory of insulation. Royal Society of Chemistry. Venturimeter. UK. thermodynamic process. SFEE. (Simple problems in SFEE).O. UNIT V: Heat Transfer with phase change: Boiling and condensation. Properties of steam. Forced convection inside tubes.

Excise Rules Unit III Factory Act and Payment of Wages Act Environmental considerations. Preliminary Project Report. Operational Aspects of Production. et al. Inventory Management. Commercial Banks. coordination and control. Factors contributing to Air. water and noise pollution standards and control.” Fundamentals of Thermal Fluid Science”. Water. Nag. Assessment of demand and supply in potential areas of growth. School of Biotechnology AN-152 a K n u r a y i n U s r e v y it Marks (40 + 60) . Reference Books: 1. Importance of Communication in business Legal Aspects of Small Business. 1993. New Delhi. “A Text book of fluid Mechanics & Hydraulic Machines”. Entrepreneurial Support System.Cengel. Laxmi Publications (1992). planning. Project Report Preparation . 3rd Edition. List of items reserved for exclusive manufacture in small scale industry. Opportunities and Threats. 2002.New Delhi.2005. 2. Entrepreneurship. Personal Protection Equipment (PPEs) for safety at work places. Patent Rules.K. National Bank for Agriculture and Rural Development (NABARD). Noise pollution. Khadi Village and Industries Commission (KVIC). R.P.Text Book: 1. Need. Small Industries Service Institutes (SISIs).. CL216 ECONOMICS AND FOOD INDUSTRY MANAGEMENT Credit 3:1:0 Unit I Profiles of Food Processing Industries in India Industrial production and growth rate in food processing sector. Sales Tax. functions of management viz. Ratio analysis. Tata McGraw Hill Publishing Company Ltd. Managerial Aspects of Small Business Unit II Principles of Management Definition. TaTA McGraw Hill.. Industrial Relations and Disputes Unit IV Preparation Of Financial Statements Classification of accounts. 3. Financial statement analysis. double book entry: Financial analysis. Elementary knowledge of Income Tax. Bansal.. Air. Procedures for registration of small scale industry. State Financial Corporations. National Small Industries Corporation (NSIC). Small Industries Development Bank of India (SIDBI). District Industry Centres (DICs). Concept/Meaning. Profitability. Concept of ecology and environment. Dhanpat Rai & Sons. Data collection for setting up small ventures. Heat Transfer by P. Competencies/qualities of an entrepreneur. Robert H. Kothandaraman.K.C. Considerations in product selection. TechnoEconomic feasibility report Project Viability. “A course in heat engines and thermodynamics”. other relevant institutions /organizations/NGOs at State level Market Survey and Opportunity Identification (Business Planning) How to start a small scale industry. Personnel Management. Human relations and performance in organization. Significance and limitations of financial statements.Turner. Yunus A. organization.

valves (Basic construction features) UNIT II Media Design and Sterilization for Fermentation processes Medium requirements for fermentation processes. cell growth and product formation. Fund flow statements.elemental balances. BS Rathore and Dr JS Saini Aapga (Editors): A Handbook of Entrepreneurship. Management Accounting 2. Working capital and changes in working capital.R. Sharma BR: Environmental and Pollution Awareness. Break even and sensitivity analysis. maintenance coefficients. preparation of cost sheets. a K n u r a y i n U s r e v y it Marks: (40+60) School of Biotechnology AN-153 . Flow Measuring Devices. New Delhi 4. available – electron balances. Capital costing. Filter Sterilization of liquid Media and air sterilization. Materials of construction. examples of simple and complex media. Sultan Chand and Sons. Satya Prakashan . radiation and chemical sterilization. mass and energy balances in microbial metabolism. and Appannaiah H. preparation of cash flow statements. degrees of reduction of substrate and biomass. Sterilization equipment – batch and continuous. Product costing.Sterilization of Liquid Media. Related financial skills Text/Reference Books 1. role of aeration and agitation in oxygen transfer. discounted cash flow analysis. New Delhi 5. Heat transfer processes in biological system. UNIT III Metabolic Stoichiometry and Bioenergetics Thermodynamics. Design and usage of commercial media for industrial fermentations. oxygen consumption and heat evolution in aerobic cultures.N. Batch and continuous Heat. metabolic heat generation. Thermal death kinetics of microorganisms. yield coefficients of biomass and product formation. Basic design and construction of a Fermentor and its ancillaries. rates of returns.: Entrepreneurship Development.Solvency and activity ratios. pay back periods. Budgeting and budgetary controls. New Delhi CL301 BIOCHEMICAL ENGINEERING Credit: 4:0:0 UNIT I Overview of Fermentation Processes General requirements of fermentation Industry. Prentice Hall of India. Stoichiometry of Cell growth and product formation . CB Gupta and P Srinivasan. Philip Kotler: Marketing Management. oxygen uptake rates and determination of oxygen transfer coefficients (kla). Significance of fund flow and cash flow statements. UNIT IV Transport phenomena in bioreactors Mass transfer in heterogeneous biochemical reaction system: Oxygen transfer in submerged fermentation processes. Panchkula Publications. Reddy P. Unit VI Project And Product Costing Elements of costing. (Haryana) 3. Depreciation analysis. Vessel Geometry.

Bioreaction Engineering (Volume 1). Ghose (Ed. 3. John Wiley (1987).up criteria for bioreactors.UNIT V Process design and operation of Bioreactors Operational modes of reactors – batch. Pauline Doran. Second Indian Reprint (2004). reactors for waste – treatment processes. fed batch. air lift and loop reactor. 4. Biochemical Engineering Fundamentals. 3. Process Computations in Biotechnology. H. (1994). 2.K. Clark.). novel bioreactors: Stirred tank. Biochemical Engineering. D. 2. Marcel Dekker Inc. Shule and Kargi. B. Tata – McGraw Hill. Harvey W. Prentice Hall. Bioprocess Engineering Calculation. (1986). and Ollis. repetitive batch. VCH (1993) 5. T. Biochemical Engineering and Biotechnology Handbook. 1995 CL302 BIOCHEMICAL ENGINEERING LAB Credit: 0:0:2 12 experiments will be notified by the HOD from time to time a K n u r a y i n U s r e v y it Marks: (50+50) School of Biotechnology AN-154 . packed – bed and hollow – fibre membrane bioreactors. Blanch and Duoglas S. F. Academic Press.).(1997) 6. & Mavituna . McGraw Hill (2nd Edition) (1993). Text Books: 1.F. Bioprocess engineering. Bailey J. Rehm and G.. Atkinson. scale. McGraw Hill.J. Biotechnology(Vol. recycle and continuous cultivation. continuous.E. Karl Schugerl. Reed (Ed. Bioprocessing). References: 1.

characterization of neuronal cells. their function. molecular and cellular organisation of neuronal differentiation. Sensors function. n u r a y i n U s r e v y it BI210 NEUROBIOLOGY AND COGNITIVE SCIENCE Marks (40+ 60) School of Biotechnology AN-155 . synapse.ADDITIONAL SUBJECTS Code BI210 BI211 BI212 BI213 BI214 BI215 BI301 BI302 BI303 BI304 BI305 BI306 BI307 BI308 BI309 BI310 BI311 BI312 BI313 BI314 BI315 BI316 BI317 BI318 BI319 BI320 BI321 Subject Name Neurobiology and Cognitive Science Nanobiotechnology Neural Networks in Bioinformatics Advanced Immunology Molecular Modeling Lab QSAR & Drug Designing lab Advanced Bioinformatics Advanced Bioinformatics Lab Advanced Computer Programming Biological Sequence Analysis and Phylogeny Structural Bioinformatics Genomics and Proteomics Molecular Modeling & Drug Design Advanced programming in Bioinformatics Molecular Modeling/QSAR/Structure Based Drug Design Biological Databases & Analysis Lab PERL Lab Biological Sequence Analysis Lab Advanced Protein Engineering Technology Enzyme and Metabolic Engineering Computational Biology System Biology and Simulations Immunology and Immunotechnology Bioinformatics and Computing Structural Genomics and Proteome Analysis C & C++ Programming Lab Advanced Programming In Bioinformatics Lab Credit 4:0:0 4:0:0 4:0:0 4:0:0 0:0:2 0:0:2 4:0:0 0:0:2 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 3:1:0 0:0:2 0:0:2 0:0:2 0:0:2 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 0:0:2 0:0:2 a K Credits: 4:0:0 Unit I: Introduction to Nervous System Central and Peripheral nervous systems Unit II: Neuro Anatomy Structure and functions of neurons. signals produced by neurons. Glial cells.

Unit III: Neurophysiology and Neuropharmacology Pharmaceutical mediator released by neurons. 2.B. Conduction of impulses by neurons.II Molecular Manufacturing – Nano simulation. Carbon Molecules – Carbon Clusters – Carbon Nanotubes – Applications of Carbon Nanotubes UNIT .Potential benefits and risks for developing countries . Quantum wells.Nanoparticles / Nanomaterials .III Introduction to physics of the solid state: Structure – energy bands – Localized particles. Beadle. Neuron in Tissue Culture.Societal implications: Possible military applications . Self Assembly and Catalysis a K n u r a y i n U s r e v y it Marks: (40 + 60) School of Biotechnology AN-156 .Studies on the implications of Nanotechnology UNIT . Haynes. Neurobiology of higher cognetive function Guilford Press 1990. Text Books: 1. Carbon Nanostructures.IV Bulk Nanostructured materials: Solid disordered Nanostructures – Nanostructured crystals Nanostructured Ferromagnetism. 1999. A. Methods of measuring properties: Atomic structure – Particle size determination – Surface structure. Optical and vibrational spectroscopy: Infrared Frequency Range – Luminescence – Nanostructures in Zeolite Cages. 1998.Intellectual property issues . 3. Is nanotechnology bad or good? . Correlation of sensory functions. Progress in Neuropharmacology and Neurotoxicology of pesticides and drugs.Journals of Nanotechnology – Challenges of Nanotechnology – Applications of Nanotechnology – National and International Nanotechnology centers – Current and Future Research in Nanotechnology – Patent in Nanotechnology UNIT . Unit IV: Neurological Disorders Pathogenesis. Wires and Dots: Preparation of Quantum Nanostructures – Size and Dimensionality Effects – Excitons – Single Electron Tunneling – Applications – Superconductivity. Behaviour in various environments. Hormones and their effect on neuronal function. Animal behaviour.Implications of nanotechnology: Health and safety implications from nanoparticles: Health issues Environmental issues .Need for regulation . Schiebel.Criticism of Nanotechnology . Genetic basis of neurological disorders Unit V: Behaviour Science Neuronal mechanism of behaviour. BI211 NANOBIOTECHNOLOGY Credits: 4:0:0 UNIT – I Overview and History of Nanotechnology – Generations of nanotechnology . Properties of Individual Nanoparticles: Metal Nanoclusters – Semiconducting nanoparticles – Rare Gas and Molecular Clusters.

Kannangara. India. M.Introduction to Neural Network Elements . 2000. J. F. Springer. 2005.Neural Networks and Genome Informatics Elsevier Science. R. Training of Neural Networks. 2003. 4. Poole Jr. M.Prinz and R. a K n u r a y i n U s r e v y it Marks: (40 + 60) School of Biotechnology AN-157 .Protein Sequence Analysis Unit V: Neural Networks in Drug Design .V Organic Compounds and polymers: Forming and Characterizing polymers – Nanocrystals – Polymers – Supramolecular structures.Summation Operation . Gasteiger . Cathy H. K.Protein Structure Prediction . A WileyInterscience puplication. and Frank J. Owens. 2000 2. Johnson.Neurons .L. Greco. Rebecca L. Charles P. CRC press. Introduction to Nanotechnology. Nanoscale Technology in Biological Systems. CRC press. Wu and Jerry W.Modeling Metabolic Energy by Neural Networks Reference books: 1.Connections between Elements .Introduction to Radial Basis Functions.B. WileyVCH.Nucleic Acid Sequence Analysis.S. Zupan. McLarty . 3.Neural Networks in Chemistry and Drug Design. Simmons and Burkhard Raguse. 2002. Smith. 2. Nanotechnology. Wilson.Simple Feed-Forward Network Unit II: Perceptrons and Multilayer Perceptrons . 1999 BI212 NEURAL NETWORKS IN BIOINFORMATICS Credits: 4 : 0 : 0 Unit I: Neural Network Basics.UNIT .. Unit III: Genome Informatics Applications – Design – Data Encoding and Neural Networks Unit IV: Applications of Neural Networks . .Transfer Functions . Nanotechnology: Basic Science and Emerging Technologies. J.Thresholding FunctionsOther Transfer Functions .Smith. Nanomachines and Nanodevices: Microelectrochemical systems – Nanaoelectromechanical systems – Molecular and Supramolecular switches Reference Books 1. Biological Materials: Biological building blocks – Nucleic Acids – Biological Nanostructures. G.

immunosuppressive drugs. 3. Wiley Liss. 2. catalytic antibodies.. pathogenic mechanism. Thymus derived lymphocytes (T cells) their classification. Lymphocytes. application of recombinant DNA technology for the study of the immune system. their origin and differentiation. anatomy of immune response. mechanisms of T cell activation. Freeman. antigen presenting cells (APC). Benjamin E and Leskowitz S. 1991. immuno electrophoresis. antigens. immunotherapy with genetically engineered antibodies Reference Books: 1. 2002. HLA and disease. langerhans cells. and function of immunoglobulin. Oxford. ELISA-principle and applications.BI213 ADVANCED IMMUNOLOGY Credits: 4 : 0 : 0 Marks: (40 + 60) Unit I: Immune System Introduction. Unit II: Humoral Immunity and Cellular Immunology B-lymphocytes and their activation. 1991. a K n u r a y i n U s r e v y it BI214 MOLECULAR MODELLING LAB Marks: (50 + 50) Credits: 0:0:2 12 experiments will be notified by the HOD from time to time BI215 QSAR & DRUG DESIGNING LAB Credits: 0:0:2 Marks: (50 + 50) 12 experiments will be notified by the HOD from time to time School of Biotechnology AN-158 . macrophages. prevention of graft rejection. application of PCR technology to produce antibodies and other immunological reagents. cytokines and their role in immune response macrophage activation and granuloma formation. their structure and classification. Graft rejection. Kuby. Auto antibodies in humans. Blackwell Scientific Publications. structure. major histrocompatibility complex. Immunology a Short Course. Essential Immunology. SDS-PAGE. Immunofloresecnce techniques Unit V: Molecular Immunology Preparation of vaccines. types of immune responses. NY. purification and synthesis of antigens. evidence and mechanisms of graft rejection. Unit IV: Antibodies & Immunodiagnosis Monoclonal and polyclonal antibodies – their production and characterization. immunoglobulin classes and subclasses. their origin and functions. production of antidiotypic antibodies. Roitt I. radio immuno assay(RIA)-principles and applications. Western blot analysis. complement and their biological functions. Immunology. Fourth edition. mechanism of phagocytosis Unit III: Immunity of infection and autoimmunity Hypersensitivity reactions.

Viva Books Ltd. telnet. 2003 2.2004 Reference Books 1. Cambridge University Press. Unit II: Sequencing Alignment and Dynamic Programming Introduction – Strings – Edit distance two strings – string similarity local alignment -gaps – Parametric sequence alignments – suboptimal alignments – multiple alignment – common multiple alignment methods. Bioinformatics : Sequence and Genome analysis. SR Pennington. Oxford University press. 2 nd edition. 1997. Introduction to databases – database search – Algorithms issues in database search – sequence database search – FASTA – BLAST – Amino acid substitution matrices PAM and BLOSUM. 2002 a K n u r a y i n U s r e v y it BI302 ADVANCED BIOINFORMATICS LAB Marks: (50+50) 12 experiments will be notified by the HOD from time to time Credit: 0:0:2 School of Biotechnology AN-159 . MJ Dunn. protein sequence and structural databases. Proteomics from Protein Sequence to Function. Basic concepts of UNIX.scope of Bioinformatics – useful sites – Bioinformatics in pharmaceutical industry – Bioinformatics orientation in IT industry Text Book 1. World wide web – search engines – finding scientific articles -Pubmed – public biological databases. 2. http. Unit V: Applications of Bioinformatics New directions of bioinformatics research – essential pre-requisites . ftp. Lesk. CBS Publishers. Dan Gusfield. Algorithms On Strings Trees And Sequences. Unit IV: Biological manipulation DNA Mapping and sequencing – Map alignment – Large scale sequencing and alignment – Shotgun – DNA sequencing – Sequence assembly – Gene predictions – Molecular predictions with DNA strings. LINUX and PERL. Unit III: Evolutionary Trees and Phylogeny Ultrasonic trees – parsimony – Ultrametric problem – Perfect phylogeny – Phylogenetic alignment – connection between multiple alignment and tree construction. David W Mount . Databanks – nucleotide. Arthur M. Introduction to Bioinformatics.BI301 ADVANCED BIOINFORMATICS Credit: 4:0:0 Marks: (40+60) Unit I: Introduction to Bioinformatics Elementary commands and Protocols. Printer on information theory.

O’ Reilly. O’Reilly. ”const” Objects and Member Function. XML and JAVA. 7. 4.Placement of Variable Declaration . 2001 5. Mark Lutz . 3. Herbertz Schildt. Unit IV: Perl PERL: Modules:. Object oriented programming:. “ this” Pointer. Tisdall. 2000. n u r a y i n U s r e v y it School of Biotechnology AN-160 .the Scope Resolution Operator the “Con Qualifier.O’Donnell. Unit V: Bioperl General Bioperl Classes. Data and control structures:Operators and Control Flow. Prentice Hall of India QUE. Alignments: AlignIO. 2003. 2005.patterns . Genscan.string matching. Analysis: Blast. Features and Location Classes.Public and Private Inheritance .Perl. storing and using modules. 2.defining. The complete reference .2nd Ed.. James D. Osborne.Class Members . 6.New Deletes Operators. 1999. Unit II : Fundamentals of Object Oriented Design Data Abstraction.Classes within Classes. Accessing a local database. 2004. Programming Python . Similarities . Programming with Java. The complete Reference Java J2SE 5 Edition.Classes. Tom Christiansen & John Orwant.Creation and Destruction of Objects Accessing Data Members returning a Reference. Classes. E Balaguruswamy. Sequences. Function/Subroutines .References and Pointers. Reference: References as Aliases . Data Types & structre. Using HTML 4.Reference as Function Parameters . a K Reference Books: 1. Regular Expressions:.Overriding Member Functions . Encapsulation. New Delhi. Programming Perl –3rd ed. New Delhi.grouping and anchoring . Derived Class and Class Derived Constructors .Virtual Functions – Polymorphism .Reference as Return Values Unit III: Introduction to classes Declaring and Using Classes . Tata Mc Graw Hill. Beginning Perl for Bioinformatics. 1999. Objects. Array of Class Objects. O'Reilly. Databases: Database Classes. Eric Ladd.Credit : 4:0:0 BI303 ADVANCED COMPUTER PROGRAMMING Marks (40+60) Unit I: C-Language: Introduction – Operator – Expressions – Variables – Input output statements – control statements – control statements – function – arrays – pointer – structures – unions – file handling – case studies.Multiple Inheritance . Methods. Brown. Mc Graw Hill. Sequence Manipulation. Larry Wall. Classes and Dynamic Memory allocation . Overload Function. J. Inheritances and PolymorphismA first look at C++: using stream for input and output: Default Function Arguments . Tata Mc Graw Hill.File handling.

Principles and concepts used in biophysical analysis of life processes . Unit IV: Predictive methods using DNA sequences. Unit II: Multiple sequence alignment – Goal of multiple sequence alignment to pair wise alignment. Tertiary structure prediction – homology modeling tool. Bioinformatics. Iterative methods – Hidden Markov models of multiple sequence alignment. (October 1998) 3. Alignment – building the data model.Sinauer Associates.Biomolecules and their interactions. Blackwell Science Inc. II ed. INC. Expressed sequence tags (ESTs)..GOR method. Algorithm and statistics of global alignment. Genome analysis and annotation. John Wiley & Sons. School of Biotechnology AN-161 .2002 a K n u r a y i n U s r e v y it BI305 STRUCTURAL BIOINFORMATICS Marks (40+60) Credit : 4:0:0 Unit I : Introduction Levels of structures in Biological macromolecules . M. Large-scale genome analysis and the computational tools. Local alignment methods. Scoring matrices. maximum parsimony method.. 2. Position – specific scoring matrices. Progressive methods – CLUSTALW. Algorithm and statistics of local alignment. Dot matrix sequence comparison. Phylogenetic software. Edward C. Molecular evolution. Unit V: Phylogenetic analysis – phylogenetic data analysis. Reference books: 1. Tree building method. Description on the software package EMBOSS – programs and its usage. Significance of sequence alignment. Baxevenis.Algorithm behind the methods. A phylogenetic approach. Predictive methods using protein sequences.Fundamentals of Molecular evolution. Distance methods. size and shape of macromolecules.Tools used for secondary structure prediction.BI304 BIOLOGICAL SEQUENCE ANALYSIS AND PHYLOGENY Credit : 4:0:0 Marks (40+60) Unit I: Pairwise alignment techniques – Global alignment. Heuristic algorithms – FASTA and BLAST. 2000. Gene prediction methods. Unit III: Predictive methods: Different secondary structure prediction methods – Chou fasman method. PILEUP. . Roderic D.basic strategies in biophysics. extraction of a phylogenetic data set. Page. Holmes. Gap penalty. Dan Graur and Wen-Hsiung Li. Prediction of RNA secondary structure. Databases for the search.

electron microscopy.. directed short gun & Clone contig approach – Primer walking. STS. Daniel. Linear and Circular Dichroism. Unit IV: Spectroscopy and methods of visualization: Absorption spectroscopy. W. Schimmel P. Credit : 4:0:0 a K Unit I: Overview – Concepts of central dogma. geometrics. 1998. light scattering. VNTRs – Physical Marker – EST. Freeman & Co. base stacking tertiary structure of nucleic acids. Curtis Johnson and P.cDNA library Unit III: Mapping and sequence assembly – genetic marker – RFLP. hydrophobic interactions and ionic interactions. polypeptide chains geometrics. Reference Book 1. enzymatic. M. basic problems. chromosome jumping. Human Genome Project . Radiation hybrids – Sequence markers – SNPs. Contig assembly.. observed values for rotation angles. 1985. Prentice Hall. Basic Biophysics for Biologists. determination of molecular structures. Nucleotide & protein sequencing methods – Chemical. base pairing. Unit III : Structural Analysis of Macromolecules Prediction of proteins structure. Shing Ho. 1998. Expression analysis – micro array approach. macromolecular diffusion. FISH. ultracentrifugation. neutron scattering. high through put method – Automated sequencing methods Unit II: Various Approaches in sequencing genome – shotgun. Vol. Kensal E.Unit II : Conformational Analysis Forces that determine protein and nucleic acid structure. Genome Marking and mapping techniques – Genomic DNA library. van Holde.H. 2. chromosome walking. hydrogen bonding. Nuclear Magnetic Resonance spectroscopy. Methods of direct visualisation. New York. disulphide bonds. general characteristics of nucleic acid structure. Principle of Physical Biochemistry. SSLP. I.Structure & Organization of Prokaryotic & Eukaryotic genome -Variation and regulation in Prokaryotic & Eukaryotic genome expression profile– Brief outlook of various genome projects and their outcome. macromolecules as hydrodynamic particles. glycosidic bond rotational isomers and those puckering backbone rotational isomers and ribose puckering forces stabilising ordered forms. Unit V: X–ray diffraction X-ray crystallography – X–ray diffraction. potential energy calculations.. STRs.R. Cantor R. nucleic acids.Transcriptome– structural & School of Biotechnology AN-162 n u r BI306 GENOMICS AND PROTEOMICS Marks (40+60) a y i n U s r e v y it . viscometry. Biophysical Chemistry. Text Book 1. II. W. Emission spectroscopy.

specialized protein sequence databases. 2-D PAGE databases.Detection and analysis of co. genomic databases. 2. Proteomics from Protein Sequence to Function. David W Mount. Some specific metabolic databases . Bioinformatics: sequence and genome analysis.and post-translational modification.functional Genomics – comparative & population genomics. 2002. PROSITE. TrEMBL. Unit IV: Introduction to Proteome . BLOCKS. a K n u r a y i n U s r e v y it School of Biotechnology AN-163 .protein species of origin . OMIM. 2004 BI307 MOLECULAR MODELING AND DRUG DESIGN Credit : 4:0:0 Marks (40+60) Unit I: Quantum mechanics and concepts in molecular modeling: Introduction – coordinate systems – potential energy surfaces – introduction to computer hardware and software – introduction to quantum mechanics – postulates – Schrodinger wave equation – hydrogen molecule – Born-Oppenheimer approximation. Unit II: Molecular mechanics and energy minimization: Empirical force field models – Bond stretching – angle bending – torsional term – nonbonding interactions – thermodynamics properties using a forcefield – derived and non derived energy minimization method – simplex – sequential univariate method – steepest descent method – conjugate gradient method.Protein N. Metabolic databases.A Brown . Genome. T.Introduction – Using single Model – time steps – Multiple steps – Setting up MD – energy conservation in MD Simulation Examples – Monte Carto – Random number generation – Difference in MD & MC.Modifications that influence protein change on 2-D PAGE . Unit V: Proteome databases: protein sequence database.proteome and technology . toxicology and Pharmaceuticals Reference: 1.cross species protein identification . PDB. Unit III: Molecular Dynamics and Monte Carto . Viva Books Ltd.Pharmacogenomics – Phylogenetics. Pennington. 2nd edition. SWISS-PROT. 2002 3. BIOS Scientific Publishers Ltd.Newton-Rapson method. 2nd edition. 2nd Edition.and Cterminal sequence tags .application of proteomics to medicine. proteomics.information and the proteome – Primary attributes for protein identification . CBS publishers.

Modules.Basic Syntax . CGI Environment Variables.Files and Streams – Applets. XML Basics.Strings .Sequence File Format conversions. 2002 BI308 ADVANCED PROGRAMMING IN BIOINFORMATICS Credits: 3:1:0 UNIT I JAVA . java. Basic I/O. 1996 2.Control Structures Arrays . Unit-V: Drug design: General approach to discovery of new drugs . Locations and Features.molecular modeling in drug design – structure based drug design – pharmacophores . Java Script.Methods and Classes : Inheritance . Proteomics: mass and pI of a peptide .util Part 1:The Collection Framework.Packages and Interfaces . I/O. lang . Regular Expressions. Control Flow. Debugging CGI programs. BLAST and FASTA parsing.QSAR. Weight Matrices and Dynamic Programming UNIT IV PYTHON: Overview. A.java.Exploring java.JDBC Architecture.Unit IV: Homology modeling: comparative modeling of proteins – comparison of 3D structure – Homology – steps in homology modeling – tools – databases – side chain modeling – loop modeling. Style Sheets. JDBC Drivers.String Handling . Standard library.Introduction to object oriented programming . GET. Connecting to Database and accessing databases . UNIT II JAVA .HTML form elements. File Manipulation. School of Biotechnology AN-164 a K n u r a y i n U s r e v y it Marks: (40 + 60) . Text Book: 1.The Applet Class . . POST & HEAD Method.Leach.Java beans. Translation: DNA to Protein. Classes.K.lead discovery – lead modification – physiochemical principles of drug action – drug stereo chemistry –drug action .3D database search – computer aided drug design – docking . Codon to amino acid. HTML Specifications and Syntax. Handling forms. XML Applications. Introduction to JDBC (Java Database Conectivity) .Threads .util Part2: More Utility Classes .Exceptions Handling -Multithreaded Programming. Data structures. Molecular Modeling Principles and Application. Longman. Quantum chemistry . UNIT V WEB DESIGNING & XML Common Gateway Interface:.Input/Output: Exploring java.Networking .io . Six frame translation.Event Handling UNIT III BIOJAVA: Introduction .Prasad. Passing Parameters via CGI.Sequence Manipulation.R. R.

Osborne. Using HTML 4. Tata Mc Graw Hill. James D. Brown. Eric Ladd. 2005. O'Reilly. Mc Graw Hill.O’Donnell. New Delhi.denaturation and coagulation of protein. 4. 2004. E Balaguruswamy. The complete reference .Perl. 2001 5. 1999.2nd Ed. 7. Tisdall. New Delhi. XML and JAVA. Programming Perl –3rd ed. O’Reilly. Isolation. J. Programming Python .Reference: 1. The complete Reference Java J2SE 5 Edition. Prentice Hall of India QUE. 2003. BI309 MOLECULAR MODELING/QSAR/STRUCTURE BASED DRUG DESIGN LAB Credits: 0:0:2 Marks: (50 + 50) 12 experiments will be notified by the HOD from time to time BI310 BIOLOGICAL DATABASES AND ANALYSIS LAB Credits: 0:0:2 Marks: (50 + 50) 12 experiments will be notified by the HOD from time to time Credits: 0:0:2 a K Credits: 0:0:2 n u r 12 experiments will be notified by the HOD from time to time a y i n U s r e v y it BI311 PERL LAB Marks: (50 + 50) BI312 BIOLOGICAL SEQUENCE ANALYSIS LAB Marks: (50 + 50) 12 experiments will be notified by the HOD from time to time BI313 ADVANCED PROTEIN ENGINEERING TECHNOLOGY Marks (40+60) Credit : 4:0:0 Unit I: Introduction of proteins Classification and properties of proteins. Larry Wall. 2.. Programming with Java. Herbertz Schildt. Beginning Perl for Bioinformatics. O’ Reilly. extraction and purification of proteins. 3. Tata Mc Graw Hill. 1999. Tom Christiansen & John Orwant. 2000. School of Biotechnology AN-165 . 6. Mark Lutz .

Moody PCE. protein phosphorylation. covalent binding etc. immunoglobulins. bacteriorhodopsin. health care and environment. Types of Inhibition-kinetic models. turnover number. N-terminal and C. IRL Press. Proteins. Tooze R. Second Edition. Oxford. Freeman WH. Protein Engineering. 1993 BI314 ENZYME AND METABOLIC ENGINEERING Credit : 4:0:0 Marks (40+60) a K Unit I: Mechanisms and Kinetics of Enzyme Action. Commercial applications of enzymes in School of Biotechnology AN-166 n u r a y i n U s r e v y it . 3. Physical and Chemical techniques for enzyme immobilization-adsorption. Specificity of enzyme action. insullin and PGDGF receptors and their interaction with effectors. Nucleotide binding proteins. Formulation of dimensionless groups and calculation of Effectiveness Factors. overview of applications of immobilized enzyme systems.terminal amino acids. tertiary structure and domain in proteins. prokaryotic and eukaryotic transcription factors.. cross-linking. 2. methods to alter primary structure of proteins. Substrate and Product Inhibition. Concept of active site and Energetics of enzyme substrate complex formation. Unit III: Enzyme Biosensors and commercial applications Applications of enzymes in analysis. Membrane proteins and receptors. examples. Deactivation kinetics. and AJ Wilkinson. protein folding pathways. 1993. Analysis of Film and Pore Diffusion Effects on Kinetics of Immobilized Enzyme Reactions. Mechanisms of Enzyme Action. Creighton TE. lysozyme. epidermal growth factor. matrix entrapment. photosynthetic centres. protein design. Kinetics of single substrate reactions. post transnational modification. Introduction of protein structure Garland. Allosteric regulation of enzymes. Unit II: Enzyme Immobilization and Mass Transfer Effects in Immobilized Enzyme System. secondary structure prediction and determination of supersecondary structures. Amino acid sequence of proteins by chemical and enzymatic methods and finger printing technique Unit III: Structure of Proteins – Primary structure and its determination.Unit II: Amino acid sequence and conformation of proteins Determination of amino acid composition. quarternary structure. example of engineered proteins. advantages and disadvantages of different immobilization techniques. estimation of Michaelis-Menten parameters. Branden C. encapsulation. 1990. DNA polymerases. Unit IV: Structure Function Relationship of Proteins DNA binding proteins. multi-substrate reactionsmechanisms and kinetics. principle and examples. enzyme serine proteases. Reference Books: 1. Design of enzyme electrodes and their application as Biosensors in industry. ribonuclese. Unit V: Protein Engineering and Protein Design Protein data base analysis.

sequence based methods. Protein folding invitro and invivo. Cornish-Bowden. contact graphs. Analysis of enzyme kinetic data. cometabolism. regulation of enzyme synthesis. Blakeborough and P. mixed or sequential bioconversions. enzyme induction.Wiseman. RNA secondary structures. avoidance of product inhibition. Unit IV: Metabolic engineering Introduction to Metabolic Engineering. Biomolecular sequence analysis – Nucleic acid sequences. 2. A. Precursor effects. Protein structure prediction . Ellis and Harwood. specificity. reaction rates. trophophase. Reference Books: 1. Advanced Sequence data description. yields. Genome annotation. idiophase relationship. catabolite regulation by passing control of secondary metabolism. Oxford Univ. UNIT .food. Topics in enzyme and fermentation Biotechnology. Enzymatic and Nonenzymatic Catalysis. Structure based method.Vol 5. and dynamic mass balances -Growth Energetics. 1995. BI315 COMPUTATIONAL BIOLOGY Credit : 4:0:0 Unit I: Introduction to computational biology Introduction. Random graph models of sequence structure maps. 1996. n u r a y i n U s r e v y it Marks (40+60) School of Biotechnology AN-167 . Ab inito method Unit IV: Computational Genomics Sequences and contigs. Press. Motifs – localization and extraction.Alignment based methods.V Metabolic Engineering in Bioconversions Metabolic bioconversion pathways. Ex.. Butterworth. Computer simulated functions. Dunhill. Protein sequence analysis and prediction of secondary structural features Unit II: Discrete models of Biopolymers Discretized structure models – Lattice proteins. pharmaceutical and other industries. conversion of insoluble substances.Yield coefficients and linear rate equations Material balances & Data consistency. mutation. Technological applications of biocatalysts. BIOTOL series. feedback regulation. 3. Enzymes for analytical and diagnostic applications. UK Vol. Combinatorial considerations – secondary structure graphs.Eukaryotic and Prokaryotic genome annotation tools. Sequence data description. a K Unit III: Protein structure folding & prediction and DNA-Protein Interaction Overview of protein structure. permeability. 4. Wiseman and N. Producers of secondary metabolites. 5. 1981. Ellis and Harwood. factors important to bioconversion. Insilico folding. UK. Theoretical models of folding.Metabolism -Stoichiometry. DNAProtein Interaction – Target prediction. Advantages of bioconversions.

Computer Simulation of the Cell: Human Erythrocyte Model and its Application . BI316 SYSTEM BIOLOGY AND SIMULATIONS Credit : 4:0:0 Unit I: Introduction . Alignment. Ralf Hofestadt (Editor). James M. Hiroaki Kitano (Editor). 1997. 2001 2. 3. Amsterdam.Stochastic Simulation of Cell Signaling Pathways -Analysis of Complex Dynamics in Cell Cycle Regulation Unit IV: Modeling Large Biological Systems from Functional Genomic Data: Parameter Estimation Cellular Simulation .Computational Methods in Molecular Biology . Hamid Bolouri. Julio Collado-Vides (Editor).System-level Understanding of Biological Systems . Compact Handbook of Computational Biology. Bower.Computational Cell Biology : The Stochastic Approach a K Unit V: Computer Simulation of the Whole Cell . 2. .Towards a Virtual Biology Laboratory . Andrezej K Konopka and James C. 2004 References 1.A Probabilistic Model of a Prokaryotic Gene and its Regulation. Visualization Text Book 1. Crabbe. Tools for comparative genomics – data selection.2002 School of Biotechnology AN-168 n u r a y i n U s r e v y it Marks (40+60) .2000. and Simon Kasif. Joao Setubal and Joao Meidanis..Unit V: Computation in Comparative Genomics Introduction.Advanced Measurement Systems Modeling Genetic Networks Unit II: Modeling the Activity of Single Gene .Software for Modeling and Simulation – E-CELL. Steven Salzberg. Boston. Introduction to Computational Molecular Biology Publisher: PWS Publishing Company. Marcel Dekker. USA. Modeling Biochemical Networks .Atomic-Level Simulation and Modeling of Biomacromolecules Unit III: Kinetic Models of Excitable Membranes and Synaptic Interactions . Foundations of Systems Biology. MIT Press. Gene Regulation and Metabolism: Postgenomic Computational Approaches. 1998. Publisher: Elsevier Science B. Computational Modeling of Genetic and Biochemical Networks.MIT Press. Evolutionary basis.V. V-CELL and GROMOS Reference books: 1. David Searls. MIT Press.

BI317 IMMUNOLOGY AND IMMUNOTECHNOLOGY Credit : 4:0:0 Marks (40+60) Unit I: Immune System Introduction, Lymphocytes, their origin and differentiation, antigens, their structure and classification, complement and their biological functions, types of immune responses, anatomy of immune response.

Unit II: Humoral Immunity and Cellular Immunology B-lymphocytes and their activation, structure, and function of immunoglobulin, immunoglobulin classes and subclasses, major histrocompatibility complex. Thymus derived lymphocytes (T cells) their classification antigen presenting cells (APC), macrophages, langerhans cells, their origin and functions, mechanism of phagocytosis

Unit III: Immunity of infection and autoimmunity Hypersensitivity reactions, mechanisms of T cell activation, cytokines and their role in immune response macrophage activation and granuloma formation., Auto antibodies in humans, pathogenic mechanism, Graft rejection, evidence and mechanisms of graft rejection, prevention of graft rejection, immunosuppressive drugs, HLA and disease Unit IV: Antibodies & Immunodiagnosis Monoclonal and polyclonal antibodies – their production and characterization, western blot analysis, immuno electrophoresis, SDS-PAGE, purification and synthesis of antigens, ELISA-principle and applications, radio immuno assay(RIA)-principles and applications, Immunofloresecnce techniques Unit V: Molecular Immunology Preparation of vaccines, application of recombinant DNA technology for the study of the immune system, production of antidiotypic antibodies, catalytic antibodies, application of PCR technology to produce antibodies and other immunological reagents, immunotherapy with genetically engineered antibodies Reference Books: 1. Roitt I, Essential Immunology, Blackwell Scientific Publications, Oxford, 1991. 2. Benjamin E and Leskowitz S, Immunology a Short Course, Wiley Liss, NY, 1991. 3. Kuby, Immunology, Fourth edition, Freeman, 2002.

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BI318 BIOINFORMATICS AND COMPUTING Marks (40+60)

Credit : 4:0:0

Unit I: Basics in Bioinformatics Elementary commands and Protocols, ftp, telnet, http, WWW. Basics of computers – Servers, Operating systems – LINUX, UNIX. Databanks – nucleotide databanks –– protein databanks – sequence databanks – structural databases.

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Unit II: Sequencing Alignment and Dynamic Programming Introduction – Strings – Edit distance two strings – string similarity local alignment -gaps – Parametric sequence alignments – suboptimal alignments – multiple alignment – common multiple alignment methods. Algorithms issues in database search – FASTA – BLAST – Amino acid substitution matrices PAM and BLOSUM. HMM and ANN. GCG Sequence Analysis.

Unit III: Phylogeny, DNA Mapping and sequencing Ultrasonic trees – parsimony – Ultrametric problem – Perfect phylogeny – Phylogenetic alignment – connection between multiple alignment and tree construction. DNA Mapping and sequencing – Map alignment – Large scale sequencing and alignment – Shotgun – DNA sequencing – Sequence assembly – Gene predictions – Molecular predictions with DNA strings. Unit IV: Graph Theory and Boolean Algebra Basic concepts – datastructure for graphs – connectivity – graph optimization. Trees – Spanning Trees – Rooted Trees – Binary Trees Boolean Algebra – Posets, Lattices – Application of Boolean Algebra to Switching theory. Unit V: Computer Programming Internet and Java Programming – Fundamental Programming Structure in Java – Use of Classes – Creating Packages String handling – IO streams – Applet Class. Specific applications in Biological computing. Programming strategies in PERL (Fundamentals only) Text Books 1. Dan Gusfield, Algorithms On Strings Trees And Sequences, Cambridge University Press, 1997. 2. James Tisdal, Beginning Perl for Bioinformatics, Relly Publications, 2003 3. Arhtur M. Lesk, Introduction to Bioinformatics, Oxford University press, 2003 References 1. Baxevenis, Bioinformatics, John Wiley & Sons, 2002 2. Westhead, DR.., Parish JH and Twyman RM, Instant notes on Bioinformatics, Viva books, New Delhi, 2003 3. David W Mount, Bioinformatics: sequence and genome analysis, CBS publishers,2004 4. Seymour Lipschutz and Lipson ML, Discrete Mathematics, McGraw Hill Publications, 1992.

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BI319 STRUCTURAL GENOMICS AND PROTEOME ANALYSIS Marks (40+60)

Credit : 4:0:0

Unit I: Genome Sequencing Structure & Organization of Prokaryotic & Eukaryotic genome - Nucleotide & protein sequencing methods – Chemical, enzymatic, high through put method – Automated sequencing methods – shotgun –chromosome walking, Contig assembly. Genome Mapping
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techniques –Mapping and sequence assembly – genetic marker – RFLP, SSLP, STRs, VNTRs – Physical Marker – EST, STS, FISH, Radiation hybrids – Sequence markers – SNPs. Expression analysis – micro array approach. Unit II: Comparative Genomics Human Genome Project - Transcriptome– structural & functional Genomics – comparative & population genomics – Pharmacogenomics..

Unit III: Proteome analysis Proteome – Bridging Genomics and Proteomics – Analysis of Proteome – 2D PAGE – Mass spectrometry – MALDI TOF – Micro array Technology – CD. Application of Proteome analysis – Protein Chips – Drug Development and Toxicology – Proteomic in genetic and breeding. Legal aspects of Genomics & proteomics – Bioethies – IPR & Patents. Unit IV: Protein structure elucidation Size and shape of macromolecules, methods of direct visualisation, X-ray crystallography – X–ray diffraction, determination of molecular structures ,electron microscopy, neutron scattering, light scattering and NMR Unit V: Structure Function Relationship of Proteins DNA binding proteins, prokaryotic and eukaryotic transcription factors, DNA polymerases, Membrane proteins and receptors, bacterio rhodopsin, photosynthetic centres, epidermal growth factor, insullin and PGDGF receptors and their interaction with effectors, protein phosphorylation, immunoglobulins, Nucleotide binding proteins, enzyme serine proteases, ribonuclese, lysozyme. Text Books 1. T.A Brown - Genome, II Edn, BIOS Scientific publishers Ltd., UK. 2002. 2. SR Pennington, MJ Dunn, Proteomics from Protein Sequence to Function, Viva books Ltd, 2002 3. David W Mount, Bioinformatics: sequence and genome analysis, CBS Publishers References: 1. Creighton TE, Proteins, Freeman WH, Second Edition, 1993. 2. Cantor R., Schimmel P.R., Biophysical Chemistry, Vol. I, II, W.H. Freeman & Co., 1985.

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BI320 C & C++ PROGRAMMING LAB Marks (50+50)

12 experiments will be notified by the HOD from time to time

BI321 ADVANCED PROGRAMMING IN BIOINFORMATICS LAB Marks (50+50) 12 experiments will be notified by the HOD from time to time

Credit : 0:0:2

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ADDITIONAL SUBJECTS Code BT214 BT215 BT216 BT217 BT218 BT219 BT220 BT221 BT301 BT302 BT303 BT304 BT305 BT306 BT307 BT308 BT309 BT310 BT311 BT312 BT313 BT314 BT315 BT316 BT317 Subject Name Protein Engineering Animal Biotechnology Cancer Biology Bioprocess Economics and Plant Design Professional Ethics and Human Values Environmental Biotechnology Advanced Bioprocess Lab Basic Industrial Biotechnology Advances in Genetic Engineering Genetic Engineering Lab Advanced Bioprocess Engineering Bioprocess Engineering and Downstream processing Lab Metabolic Regulations and Engineering Advanced Immunotechnology Immunotechnology Lab Plant and Animal Biotechnology Environmental Biotechnology Professional Ethics And Bioethics Advanced Biopharmaceutiucal Technology Enzyme Technology and Industrial applications Research Methodology Instrumentation and Biotechniques Plant and Animal Biotechnology for Food Engineers Metabolic Engineering Downstream Processing Credit 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 0:0:2 4:0:0 4:0:0 0:0:2 4:0:0 0:0:2 4:0:0 4:0:0 0:0:2 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 3:1:0 3:1:0 3:1:0

Credit : 4:0:0

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UNIT I Bonds And Energies In Protein Makeup Covalent, Ionic, Hydrogen, Coordinate, hydrophobic and Vander walls interactions in protein structure. Interaction with electromagnetic radiation (radio, micro, infrared, ultraviolet, Xray) and elucidation of protein structure. UNIT II Amino Acids And Their Characteristics Amino acids (the students should be thorough with three and single letter codes) and their molecular properties (size, solubility, charge, pKa), Chemical reactivity in relation to posttranslational modification (involving amino, carboxyl, hydroxyl, thiol, imidazole groups) and peptide synthesis.

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BT214 PROTEIN ENGINEERING Marks (40+60)

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UNIT III Protein Architecture Primary structure: peptide mapping, peptide sequencing - automated Edman method & massspec. High-throughput protein sequencing setup Secondary structure: Alpha, beta and loop structures and methods to determine Super-secondary structure: Alpha-turn-alpha, beta-turn-beta (hairpin), beta-sheets, alphabeta-alpha, topology diagrams, up and down & TIM barrel structures nucleotide binding folds, prediction of substrate binding sites Tertiary structure: Domains, folding, denaturation and renaturation, overview of methods to determine 3D structures, Quaternary structure: Modular nature, formation of complexes.

UNIT IV Structure-Function Relationship DNA-binding proteins: prokaryotic transcription factors, Helix-turn-Helix motif in DNA binding, Trp repressor, Eucaryotic transcription factors, Zn fingers, helix-turn helix motifs in homeodomain, Leucine zippers, Membrane proteins: General characteristics, Transmembrane segments, prediction, bacteriorhodopsin and Photosynthetic reaction center, Immunoglobulins: IgG Light chain and heavy chain architecture, abzymes and Enzymes: Serine proteases, understanding catalytic design by engineering trypsin, chymotrypsin and elastase, substrate-assisted catalysis other commercial applications. UNIT V Protein Engineering Advantages and purpose, overview of methods, underlying principles with specific examples: thermal stability T4-lysozyme, recombinant insulin to reduce aggregation and inactivation, de novo protein design. Text Books 1. Voet D. and Voet G., “Biochemistry”, Third Edn. John Wiley and Sons, 2001 2. Branden C. and Tooze J., “Introduction to Protein Structured, Second Edition”, GarlandPublishing, NY, USA, 1999 References 1. Creighton T.E. Proteins, Freeman WH, Second Edition, 1993 2. Moody P.C.E. and Wilkinson A.J. “Protein Engineering”, IRL Press, Oxford, UK, 1990.

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UNIT I Animal Cell Culture Introduction to basic tissue culture techniques; chemically defined and serum free media; animal cell cultures, their maintenance and preservation; various types of culturessuspension cultures, continuous flow cultures, immobilized cultures; somatic cell fusion; cell cultures as a source of valuable products; organ cultures. UNIT II Animal Diseases And Their Diagnosis Bacterial and viral diseases in animals; monoclonal antibodies and their use in diagnosis; molecular diagnostic techniques like PCR, in-situ hybridization; northern and southern blotting; RFLP.
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UNIT III Therapy Of Animal Diseases Recombinant cytokines and their use in the treatment of animal infections; monoclonal antibodies in therapy; vaccines and their applications in animal infections; gene therapy for animal diseases. UNIT IV Micromanipulation Of Embryos What is micromanipulation technology; equipments used in micromanipulation; enrichment of x and y bearing sperms from semen samples of animals; artificial insemination and germ cell manipulations; in vitro fertilization and embryo transfer; micromanipulation technology and breeding of farm animals.

UNIT V Transgenic Animals Concepts of transgenic animal technology; strategies for the production of transgenic animals and their importance in biotechnology; stem cell cultures in the production of transgenic animals. Text Books 1. Ranga M.M. Animal Biotechnology. Agrobios India Limited, 2002 2. Ramadass P, Meera Rani S. Text Book Of Animal Biotechnology. Akshara Printers, 1997. Reference 1. Masters J.R.W. Animal Cell Culture: Practical Approach. Oxford University Press, 2000

Credit : 4:0:0

UNIT I : Fundamentals Of Cancer Biology Regulation of cell cycle, mutations that cause changes in signal molecules, effects on receptor, signal switches, tumour suppressor genes, modulation of cell cycle in cancer, different forms of cancers, diet and cancer. Cancer screening and early detection, Detection using biochemical assays, tumor markers, molecular tools for early diagnosis of cancer. UNIT II : Principles Of Carcinogenesis Theory of carcinogenesis, Chemical carcinogenesis, metabolism of carcinogenesis, principles of physical carcinogenesis, x-ray radiation-mechanisms of radiation carcinogenesis. UNIT III : Principles Of Molecular Cell Biology Of Cancer Signal targets and cancer, activation of kinases; Oncogenes, identification of oncogenes, retroviruses and oncogenes, detection of oncogenes. Oncogenes/proto oncogene activity. Growth factors related to transformation. Telomerases. UNIT IV : Principles Of Cancer Metastasis Clinical significances of invasion, heterogeneity of metastatic phenotype, metastatic cascade, basement membrane disruption, three step theory of invasion, proteinases and tumour cell invasion.

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Oxford.W. prediction of aggressiveness of cancer.S. UNIT V : Quality And Quality Control Current good manufacturing practices. Design of Variables. Market Survey. Process Flow sheets. Maly B. Elements of quality control envisaged by BIS since 1947. chemotherapy. Balance Sheets. Peters M. j Oxford Medical Publications. Oxford. Capital Costs. 2001. Profitablity Standards.J. UNIT III : Cost Estimation. Sourcing of Processes.. Use of signal targets towards therapy of cancer. 2. Six sigma concept in Quality control Text Books 1. Reference 1. “Introduction to Modern Virology”. Preparation of Budgetory investment and production costs. Fundamental Concepts of ISO 9000 Quality System and the various requirements for ISO certification. Depreciation. Fixing most economic processes.. BT217 BIOPROCESS ECONOMICS AND PLANT DESIGN Credit : 4:0:0 UNIT I : Process Economics And Business Organizations Definition of Process. Alternative investments and Replacements. Concept of time-Value of money. Structure and Types of Organizations UNIT II : Project Design And Development Choosing a Project. Plant Lay out.B. Working Capital. Process alternatives. Gene therapy.J And Primrose S.Project profile concept-details. Source Sink concept of Profitability. Profitability And Accounting Capital investment.UNIT V :New Molecules For Cancer Therapy Different forms of therapy. radiation therapy. 2. Text Books 1. 1991. Performance Analysis. Plant Design and Economics for Chemical Engineers. Senapathy R. Lakshmi Publications. 1988. McGraw-Hill International Edition. Manufacturing Costs. “An Introduction Top Cellular And Molecular Biology of Cancer”. advances in cancer detection. Annual reports. Process Economics. Emergence of Statistical Process Control (SPC). Importance of various M-inputs-Globalization concept-Competition by Dumping-It’s effect on Plant size-Status of India. detection of cancers. Blackwell Scientific Publications. IRLl Press. TechnologyScanning. Project profitability evaluation. “Virology A Practical Approach”. Klaus D. 1991. Chemical Engineering series. 1987. UNIT IV : Process Optimization Techniques Optimum design-Design Strategy. Dunmock N. Plant Location Principles. Importance of Techno-Economic-Feasibility Studies. School of Biotechnology AN-175 a K n u r a y i n U s r e v y it Marks (40+60) . Concepts of Quality Control in 20th century. Economic-Balance. Estimation of Capital costs. Simple SPC concept details. Text Book of Principles of Management and Industrial Psychology.

“Engineering Ethics – Concepts and Cases”. Protchard and Michael J Rabins.theories about right action . Wadsworth Thompson Learning. S. “ Engineering Ethics”.the three mile island and Chernobyl case studies. Rudd and Watson.discrimination. References 1.weapons development .a balanced outlook on law . Strategy for Process Engineering.1987.assessment of safety and risk .Intellectual Property Rights (IPR) . Senthil Kumar V. Pearson Education / Prentice Hall.customs and religion .consensus and controversy – Models of Professional Roles . Michael S.variety of moral issued . UNIT III : Engineering as Social Experimentation Engineering as experimentation . 3.engineers as managers-consulting engineers-engineers as expert witnesses and advisors moral leadership-sample code of Ethics (Specific to a particular Engineering Discipline). Collegiality and loyalty . New York 1996. Responsibilities and Rights Safety and risk .Reference 1. United States.codes of ethics .moral dilemmas .computer ethics . 2000 (Indian Reprint now available) School of Biotechnology AN-176 n u r a y i n U s r e v y it . BT218 PROFESSIONAL ETHICS AND HUMAN VALUES Credit : 4:0:0 Marks (40+60) UNIT I : Human Values Morals.collective bargaining .respect for authority . New Delhi. 2004.professional rights . Mike Martin and Roland Schinzinger.moral autonomy .the challenger case study UNIT IV : Safety. Charles D.types of inquiry .Gilligan's theory .Environmental ethics . Charles E Harris. Fleddermann.uses of ethical theories. 2004 (Indian Reprint now available) 2. “Engineering Ethics”.risk benefit analysis and reducing risk . Wiley Publications.occupational crime .engineers as responsible experimenters . Values and Ethics – Integrity – Work Ethic – Service Learning – Civic Virtue – Respect for Others – Living Peacefully – caring – Sharing – Honesty – Courage – Valuing Time – Co-operation – Commitment – Empathy – Self-Confidence – Character – Spirituality. UNIT V : Global Issues Multinational corporations .Self-interest .employee rights . New Jersey. UNIT II : Engineering Ethics Senses of “Engineering Ethics”' .confidentiality conflicts of interest . a K Text Books 2. Govindarajan M. Natarajan S. “Ethics in Engineering”.Kohlberg's theory . McGraw-Hill. Prentice Hall of India.

L.R. Wheelis M. John ware D. John R Boatright. Oxford. Environmental Pollution soil. 1989. Environmental Biotechnology. Unit III : Biomass Types and composition of biomass. Vol. sugar and industry pulp. Painter R. Unit IV : Bioremediation and biodegradation Pseudomonas for bioremediation.A. waste as renewable source of energy.3. tannery. “Fundamentals of Ethics for Scientists and Engineers”. 2003. BT219 ENVIRONMENTAL BIOTECHNOLOGY Credit : 4:0:0 Marks (40+60) Unit I : Introduction Origin and Definition of old and new biotechnology. General Microbiology. London 1989. Chakrabarty K. methods of energy production. 1987. microbes in leaching of metals – leaching of copper and uranium. types and reactions of bioremediation. control devices for gaseous pollutants. McMillan Publications. Oxford University Press. Types and causes and its effects on environment. Intellectual Property Rights (IPR) and patenting – patents for higher plants and animals Text Books 1. Microbial flora of soils .Biodiversity and its conservation – insitu and exsitu conservation. “Ethics and the Conduct of Business”. Unit II : Industrial waste water management Aerobic and anaerobic waste water treatment. water air. cellulose as a source of energy.Y. 2. Pearson Education. conservation of energy. Ellis. Loss of biodiversity and its causes. New Delhi. conversion of methane to synthetic gas. energy and fuel using microorganisms. Edmund G Seebauer and Robert L Barry. Control of air pollution. Honwood Ltd. Unit V : Biodiversity Biodiversity at global level.S. a K n u r a y i n U s r e v y it School of Biotechnology AN-177 . biodegradation of hydrocarbons and pesticides. sewage disposal and treatment – physical and biological treatment. volatile organic pollutants. Foster C. Gulf Publications Co.F. Advances in Applied Biotechnology series. effluent treatment – primary and secondary treatment. Stanir R. Biotechnology a multidisciplinary growing tree.L. scope and importance of Biotechnology in India. oil and heavy metal pollution. Biological nitrogen and phosphate removal . Reference 1. Ingraham J. 4. Ovnen G. Biotechnology and Biodegradation. 2001. Karnely D.waste water treatment in diary. distillery. species diversity.

Presscott. PHB and Single cell protein. 3. Biofertilisers. Industrial Microbiology. Aminoglycosides: streptomycin. Biopreservatives: Nisin. lysine. Production of Biopesticides. Pergamon. (2003) Biotechnology: A Textbook of Industrial Microbiology. IInd edition. amylases. UNIT – V Production of Modern Biotechnology Products Production of recombinant proteins having therapeutic and diagnostic applications: vaccines. processes. Agrobios (India). UNIT – II Production of Primary Metabolites A brief outline of processes for the production of some commercially important Organic acids: citric acid. Biopolymers: xanthan gum. B2. monoclonal antibodies. Amino acids: glutamic acid. lipases. Cheese. cellulases and Enzyme Inhibitors: inhibitors of cholesterol synthesis. Vitamins: Vit. Products of plant and animal cell cultures.B12. L. 2. Cambridge University press. Vit. Process flow sheeting – block diagrams. New Age International (P) Ltd. (2000) Industrial Microbiology. UNIT – IV Production of Enzymes and Other Bioproducts Production of Industrial Enzymes: proteases. Panima Publishing Corporation. Comprehensive Biotechnology. Dunn. aspartic acid and Alcohols: ethanol. products relating to modern biotechnology. Casida Jr. Wulf Cruger and Anneliese Crueger. UNIT – III Production of Secondary Metabolites Study of production processes for various classes of secondary metabolites: AntibioticsBeta-lactams: penicillin. Macrolides: erythromycin.E.A and Steroids. 2004..BT220 ADVANCED BIOPROCESS LAB Credit : 0:0:2 Marks (50+50) 12 experiments will be notified by the HOD from time to time BT221 BASIC INDUSTRIAL BIOTECHNOLOGY Credit : 4:0:0 Marks (40+60) UNIT – I Introduction to Industrial Bioprocess A historical overview of industrial fermentation process – traditional and modern biotechnology. Text Book: 1. acetic acid. cephalosporin. Ratledge & Kristiansen. butanol. A brief survey of organisms. pictorial representation. References 1. 2. Murrey Moo & Young. Basic Biotechnology. phenyalanine. a K n u r a y i n U s r e v y it School of Biotechnology AN-178 . lactic acid.

IV Techniques in Genetic Engineering Introduction to DNA finger printing.Raymond .S. Applications of genetic engineering.Brown.David Rawn & K. unfused genes. 2002. transposon tagging.A.K. 2. Site specific recombination. "Principles of Biochemistry" by H. Keith H. Gene targeting. personal identification.DNA engineering – oligonucleotide synthesis. DNA foot printing.Robert Horton. chromosome walking.S.III Sequencing of Nucleic Acid DNA sequencing – MAXAM and GILBERT chemical method.Ltd. UNIT. Directed mutagenesis. Academic Press. restriction mapping – direct mapping.Genetic Engineering – Hazards and Impact on society.II Gene Cloning Gene library in a genomic library. indirect mapping. Panima Publishing Corporation. Oxford University Press.A. 3.West. Prentice Hall. phage lambda versus cosmids for gene libraries. Michael D. expression in transgenic animals and plants. 2000. Reference Books 1. chromosome jumping. 2. Hybridization based DNA fingerprinting (RFLP) PCR-based DNA fingerprinting. Principles of cloning by Jose Cibelli. Paternity dispute etc. how to isolate a gene – plasmid and marker rescue techniques. Cambridge. Genes VI and Genes VII by Benjamin Lewin. cDNA library. UNIT. Jodhpur.1988. adopters and connectors. gene banks. Messing’s shotgun method using computers for sequencing. SANGER and COULSON enzymatic chain termination method – the primer. 3rd ed. the polymerase. 2003. 2002.Ochs. Applications of DNA fingerprinting Criminal investigation. Short Tandem Repeats (STRs)/ Microsatellite sequences.S. prokaryotic gene expression invitro.I Molecular Biology Prokaryotic gene expression invivo. Agrobios(Ind). Campbell. "From Genes to Clones" by Ernat-. 7th ed. UNIT.Lanza. basic genetic principle. Single locus and multi-locus DNA fingerprinting . linkers.coli – fused genes. J. Moran .K) Co. transposon mutagenesis. "Biotechnology : Fundamentals and Applications" by S. India. mapping the DNA.V Applications and Hazards of Genetic Engineering Exploitation of genetic engineering. secreted proteins. the template. Gray Scrimgeour.Robert P. School of Biotechnology AN-179 a K n u r a y i n U s r e v y it . Variable Number of Tandem Repeats (VNTRs)/ Minisatellite sequences.2002. 4. UNIT. Lawrence . Eukaryotic gene expression in E.VNR (U. U. Purohit .BT301 ADVANCES IN GENETIC ENGINEERING Credit: 4:0:0 Marks: (40+60) UNIT.L. Text Books: 1. mutagenesis. the dideoxynucleotide termination and deoxynucleotides.Winnacker. England. Immigration. "Gene Cloning – An Introduction" by T.

internal mass transfer in heterogenous reactions. Unit.concentration gradients and reaction rates in solid catalysts. Bioprocess Engineering. Bioprocess Engineering Principles – Pauline M Doran.V Process Control Online data analysis of physico chemical parameter measurements for biochemical processes.I Data Analysis in bioprocess Collection and analysis of data on bioprocess engineering. 1995. Biochemical Engineering Fundamentals – 2nd edition Bailey and Ollis. Wiley-Interscience.II Overview of Fermentation Processes and Scale-up Criteria Overview of fermentation industry. minimizing mass transfer effects. 1994 2. Unit. Case studies on bioreactor control problems. Scale-up criteria for mixing.Application of Artificial Intelligence. enzyme deactivation kinetics. Nancy A. Fluid flow and mixing.power requirements for agitation.M. Prentice Hall 1992 School of Biotechnology AN-180 a K n u r a y i n U s r e v Marks: (40+60) y it . Concepts of process control.determination of oxygen transfer coefficients. Unit.dynamics of multiple interacting microbial species.Lee .solid – liquid mass transfer correlations.IV Bacterial growth kinetics Simple unstructured and structured kinetic models for growth of bacterial and filamentous organisms.BT302 GENETIC ENGINEERING LAB Credit: 0:0:2 Marks: (50+50) 12 experiments will be notified by the HOD from time to time BT303 ADVANCED BIOPROCESS ENGINEERING Credit: 4:0:0 Unit. Prey. general requirements of fermentation processes. mass and energy balances for steady state and unsteady state biochemical systems.III Reaction Kinetics in bioprocess Reaction kinetics for biological systems. Fuzzy logic and neural networks in bioprocess control. main parameters to be monitored and controlled in fermentation processes. D'Elia (Editor).Predator interaction oscillations. by Bjorn K. Thiele modules . mass transfer in biological systems. Unit. Lydersen (Editor). growth associated product formation kinetics. basic configuration of fermentor and ancillaries. mass transfer correlations. Academic Press. Mcgraw Hill 1986 Reference Books 1. Kim L. formulation of models for various biochemical processing systems in steady and unsteady state conditions. 2.M kinetics. heterogenous reactions in bioprocessing. Nelson (Editor). 1st edition. Biochemical Engineering by James M. Textbooks: 1. Design and analysis of packed bed & membrane bioreactors. sterilization of air and media.

cellulases etc. feedback regulation.catabolite regulation. Regulation of gene expression in eukaryotes. Butterworth – Heinemann. Permeability control: passive diffusion. vitamins and steroids. specificity. 1993. catabolite regulation by passing control of secondary metabolism. a K UNIT – IV Metabolic Engineering of Secondary Metabolites Producers of secondary metabolites.Scragg. recognising growth cycle peak. active transport. mutation. cometabolism. 1991.. permeability. mixed or sequential bioconversions. UNIT – II Metabolic Regulations Operon concept-Regulation of gene expression in prokaryotes-Lac operon. idiophase relationship.3. mutants resistant to repression.) macrolides (erythromycin). Regulation of RNA synthesis by amino acid. Biotol Series. group transportation.I Overview of Primary and Secondary Metabolic Pathways A brief outline of processes for the production of some commercially important primary metabolites such as amino acids and alcohols. feedback resistant mutants. gene dosage. yields. n u r a y i n U s r e v y it Marks: (40+60) School of Biotechnology AN-181 . Advantages of bioconversions. Bioreactor Design and Product Yield . alteration of permeability for metabolites. lipases. UNIT . avoidance of product inhibition. factors important to bioconversion. induction. regulation of enzyme synthesis. Precursor effects.and enzyme inhibitors such as inhibitors of cholesterol synthesis.. trophophase. Industrial enzymes such as proteases.V Metabolic Engineering in Bioconversions Metabolic bioconversion pathways. concerted feed back regulation.differential regulation by isoenzymes. regulation in branched pathways. improving fermentation. Feed back regulation. aminoglycosides (streptomycin etc. UNIT –III Metabolic Engineering of Primary Metabolites and enzymes Primary metabolites: Alteration of feed back regulation. catabolite repression. BT304 BIOPROCESS ENGINEERING & DOWNSTREAM PROCESSING LAB Credit: 0:0:2 Marks: (50+50) 12 experiments will be notified by the HOD from time to time BT305 METABOLIC REGULATIONS AND ENGINEERING Credit: 4:0:0 UNIT . amylases. various classes of secondary metabolites such as secondary metabolites: antibiotics: beta-lactams (penicillin. limiting accumulation of end products.H. Enzymes: Strain selection. enzyme induction. cephalosporin etc. conversion of insoluble substances. Bioreactors in Biotechnology – A practical approach by A.). cumulative feed back regulation. feedback repression. 4.

Lilly M. Radio Immuno Assay (RIA) – Principles and applications. their structure. cytotoxicity assay.. immunotherapy with genetically engineered antibodies. Stephen J.. Peter F. UNIT IV Immunopathology Preparation of storage of tissues. Zubay G. immunoenzymatic and immunoferrtine techniques.K. T-cell activation parameters. Blackwell Publications. 1980. " Fermentation And Enzyme Technology ". non isotopic methods of detection of antigens – enhanced chemiluminescence assay.. 2004. peptide antigens preparation. application of recombinant DNA technology for the study of the immune system production anti idiotypic antibodies. immuno electron microscopy. ELISA – Principle and application.cell assay.. and Gupta S. Demain A. Macmillan Publishers. estimation of cytokines. UNIT III Techniques to assay Cell Mediated Immunity Identification of lymphocytes and their subsets in blood..M. " Biochemistry ". functional studies on isolated cells. 2005 References 1. Text Books: 1. Talwar G.. Cooney C. identification of antigens by molecular techniques UNIT II Antibodies and Immunodiagnosis Recombinant monoclonal and polyclonal antibodies – their production and characterization.. identification of various cell types and antigens in tissues.cell assay. Ltd. P. SDS-PAGE. isolation and characterization of cell types from inflammatory sites and infected tissues. application of PCR technology to produce antibodies. Weir D.D.. 2. 1990. cytotoxic T. BT306 ADVANCED IMMUNOTECHNOLOGY Credit: 4:0:0 UNIT I Antigens Types of antigen. macrophages activation. Immunoeletrophoresis.. adjuvants and their mode of action. Western Blot analysis. A Hand book of Practical and Clinical Immunology.E.C.L. application of the above technology to understand the pathogenesis of infectious diseases and treatment. Humphery A. Vol.Text books 1. Butterworth – Heinemann An Imprint of Elsevier India Pvt. Wang D...I. 2. ELISA and ELISPOT UNIIT V Molecular Immunology Preparation of vaccines. Second Edition. 1989.. invitro experimentation. helper T.L. handling of animals. CBS Publications. macrophage microbicidal assays. preparation of antigen for raising antibodies.P. Hall & A. Stanbury. John Wiley And Sons. Purification and synthesis of antigens. Practical Immunology. School of Biotechnology AN-182 a K n u r a y i n U s r e v Marks: (40+60) y it . Whitaker. immunocytochemistry – immunofluorescence. Dunnil. catalytic antibodies. Principles of Fermentation Technology. 1 & 2.

M. Biotechnology –basic needs of human beings(food. mapping of human Genome. proto – oncogenes and tumor suppressor genes. patenting genetically engineered animals.References: 1. production of Transgenic plants. Bioinsecticdes and Biofertilizers UNIT – III Plant Tissue Culture in Biotechnology Tissue culture as a source of genetic variability: somaclonal and gametoclonal variant selection sources and causes of variation. transposons.J. a K UNIT – IV Animal Cell Culture and Gene Cloning in Biotechnology Basic techniques of animal cell culture and their applications. gene cloning and restriction fragment length polymorphisms. selection of hybrid cells and regeneration of hybrid plants. R. intellectual property Rights (IPR) and patenting Text Books 1. flavors. Hudson Practical Immunology . Westwood. 2002. In vitro fertilization and embryo transfer. Ti plasmid and vectors. 2. AIDS. transgenic animals. cancer – oncogenes. Blackwell Publishers. gene cloning techniques for mammalian cells. 1990. School of Biotechnology AN-183 n u r a y i n U s r e v Marks: (50+50) y it Marks: (40+60) . Vaccines and hormone signal transduction UNIT – V Animal Genetic Engineering and Ethical Issues Techniques for gene transfer in animals. Paul N. Oxford. Frank C. Plant Tissue Culture : Applications and Limitations. Bojwani. insecticides. nif-gene transfer. anticancer agents and pharmacologically important compounds. Role of Biotechnology in third world countries. ethical issues in Animal Bio technology. Molecular biology of nitrogen fixation. application in crop improvement. culture and fusion. Austin J. perfumes. BT307 IMMUNOTECHNOLOGY LAB Credit: 0:0:2 12 experiments will be notified by the HOD from time to time BT308 PLANT AND ANIMAL BIOTECHNOLOGY Credit: 4:0:0 UNIT –I Introduction Biotechnology: Scope of Biotechnology. protoplast isolation. herbicide resistance and stress tolerance in plants. and Wood K. shelter & medicine) UNIT – II Plant Genetic Engineering Genetic Engineering – Techniques for the insertion of foreign genes into plant cells. 1st edition. and insertional mutagenesis. xenotransplantation.. Nelson. S. Olwyn M. 1993. Oxford University Press. Hay. somatic hybridization and Cybridizatoin – plant cell cultures for the production of useful chemicals : pigments. Principles of Cellular and Molecular Immunology.S.

Vol. A. scope and importance. Chakrabarty K. methods of energy production. Biotechnology a multidisciplinary growing tree. Foster C. Genetically engineered microbes for bioremediation.E. pesticides. water. control devices for gaseous pollutants. 1. Grierson. Laboratory methods for the reduction of coli form organisms in water. air. bioremediation of contaminated land – insitu and exsitu bioremediation. Environmental Biotechnology.Cambridge Reference 1. species diversity.D. energy and fuel using microorganisms. control of oil pollution. London 1989. Advances in Applied Biotechnology series. Mechanism of composting.Amsterdam 3. John Ware D.A.D. 1987. Cambridge University press. management of radio active pollutants in water. Kirsop 1990 Living Resources for bio technology 4. oil and heavy metal pollution. Omen G. volatile organic pollutants. Water recycling methods. Hay and B. Ellis Horwood Ltd. cellulose as a source of energy. microbial recovery of petroleum. biological oxygen demand. Purification of waste bleach water. Elsevier. 2.N. Green house gases and its control. Types and causes and its effects on environment. Unit IV : Bioremediation and biodegradation Types and reactions of bioremediation.Biodiversity and its conservation – insitu and exsitu conservation. BOD sensors. conversion of methane to synthetic gas. Text Books 1. a K Unit V : Biodiversity Biodiversity at global level.R. Environmental Pollution soil. herbicides. insecticides and xenobiotics. Doyle. Management of sludge and solid waste treatment – land filling. of Biotechnology in India.S. biodegradation of hydrocarbons.2. Gulf Publications Co. Plant breeders rights and farmers rights. Biotechnology and Bio degradation. convey : plant Biotechnology in Agriculture BT309 ENVIRONMENTAL BIOTECHNOLOGY Credit: 4:0:0 Unit I : Introduction Origin and Definition of old and new biotechnology. animals genes and DNA sequences. and S. n u r a y i n U s r e v Marks: (40+60) y it School of Biotechnology AN-184 . Microbial flora of soils . Unit III : Biomass Biomass waste as renewable source of energy. lagooning. Control of air pollution. Unit II : Industrial waste water management Aerobic and anaerobic waste water treatments.F. Intellectual Property Rights (IPR) and patenting – patents for higher plants. Loss of biodiversity and its causes. conservation of energy. bioremediation of ground water.

Kohlberg's theory . Biodiversity and farmers rights UNIT III Bioethics And Biosafety Bioethics.engineers as managers-consulting engineers-engineers as expert witnesses and advisors moral leadership-sample code of Ethics (Specific to a particular Engineering Discipline).L. Wheels M. Painter R. Mike Martin and Roland Schinzinger. “Ethics in Engineering”.Y.risk benefit analysis and reducing risk – the three mile island and Chernobyl case studies. Responsibilities And Rights Safety and risk . Pearson Education / Prentice Hall.variety of moral issued .types of inquiry .weapons development . Fleddermann. New Delhi. New York 1996. UNIT I Intellectual Property Rights And Patenting Intellectual Property Rights (IPR) .Self-interest .discrimination. New Jersey.. & Ollis.References 1.uses of ethical theories.consensus and controversy – Models of Professional Roles . Prentice Hall of India. Govindarajan M. Engg.theories about right action . Longman. Bailey J. S. 1999. Charles D. McMillan Publications. 3. References 1. 2. 1989.computer ethics . D. 2nd Ed. 2.F. “Engineering Ethics”.professional rights .employee rights UNIT V Global Issues Multinational corporations . Bioterrorism.moral autonomy .L. Legal implications. 1986.respect for authority .confidentiality conflicts of interest . Biochemical Engineering Fundamentals. McGraw-Hill.Environmental ethics . GMOs – Transgenic plants and Animals UNIT IV Safety. Stanier R.Gilligan's theory . General Microbiology. 2004 (Indian Reprint now available) School of Biotechnology AN-185 a K n u r a y i n U s r e v Marks: (40+60) y it . 2004. International conventions patents and methods application of patents.moral dilemmas .assessment of safety and risk . Natarajan S. Mc Graw Hill. Collegiality and loyalty .collective bargaining . Senthil Kumar V. Ingraham J. BT310 PROFESSIONAL ETHICS AND BIOETHICS Credit: 4:0:0 UNIT I Engineering Ethics Senses of “Engineering Ethics”' . Environmental Biotechnology. Biosafety issues .occupational crime . “ Engineering Ethics”.R. Text Books 1.E. Publication & Distributors Harlow.pesticide residues.customs and religion . Alam Scragg.

Saunders. Drying. Wu. analgesics. 4. Biotechnology and Biopharmaceutical Manufacturing. Singh K. mixing. BCIL. a K n u r a y i n U s r e v y it Marks: (40+60) School of Biotechnology AN-186 . Charles E Harris. Remington’s Pharmaceutical Sciences. Mack Publishing and Co. uses and Economics. “Engineering Ethics – Concepts and Cases”. Text Books 1. antibiotics. CRC Press.2. Vincent L. John R Boatright. Pearson Education. Oxford. Reference: 1. W. UNESCO Publications 1988 6.C.I: Introduction Development of Biopharmaceutical Industry. Good manufacturing practice. 1990. “Ethics and the Conduct of Business”. 2. Sasson A. Packing – Packing Techniques – Quality control . Wadsworth Thompson Learning. Edmund G Seebauer and Robert L Barry. by Kenneth E. 5. BT311 ADVANCED BIOPHARMACEUTIUCAL TECHNOLOGY Credit: 4:0:0 UNIT. “Intellectual Property Rights on Biotechnology”. New Delhi. 2001. contraceptives. 1996.B.Guyton. animal and microbial derived therapeutics like vitamins. Processing. New Delhi. United States. Michael S. Oxford University Press. A. hormones and biologicals. “Fundamentals of Ethics for Scientists and Engineers”. UNIT.V: Quality Control and Quality Assurance of Dosage Forms Analytical methods and tests for various drugs and Pharmaceuticals. and Preservation (Drug Manufacturing Technology Series. Filtration. UNIT.III: Unit Operations in Biopharmaceutical Technology Production of water for biopharmaceutical use.II: Biologic drug Metabolism and Pharmacokinetics Biologic Drug metabolism – Physico Chemical principles – Pharma Kinetics – Action of drugs on human bodies. laxatives. Avis (Editor). Hong Kong 1986. Protchard and Michael J Rabins. Vol 2). Text book of medical physiology. UNIT. 2003.IV: Biopharmaceuticals Various categories of plant. 2000 (Indian Reprint now available) 3. “Biotechnologies and Development”. Therapeutic agents. Freeze drying. Sterilisation UNIT.

Use of enzymes in analysis-types of sensing-gadgetry and methods. 3rd ed. Unit –III Mass transfer effects in immobilised enzyme systems Analysis of film and Pore diffusion Effects on kinetics of immobilised enzyme reactions. PHI. McGraw Hill. 1997 2. Hand book of Enzyme Biotechnology. Biochemical Engineering Fundamentals.S. Biochemical Engineering.F. bio safety. Bioconversion studies with immobilized enzyme packed -bed reactors. Alan.E. 2 Ed. Text Books: nd 1. a K References: 1. the research process for applied and basic research. James M.BT312 ENZYME TECHNOLOGY AND INDUSTRIAL APPLICATIONS Credit: 4:0:0 Marks: (40+60) Unit –I Kinetics and mechanism of enzyme action Classification of enzymes. Wiseman. Unit –V Enzyme biosensors Applications of enzymes in analysis. Formulation of dimensionless groups and calculation of Effectiveness Factors Unit –IV Application of Enzymes Extraction of commercially important enzymes from natural sources. School of Biotechnology AN-187 . Lee.. kinetics of inhibition. Hypothesis development. Analysis of Film and Pore Diffusion Effects on Kinetics of immobilized enzyme reactions. healthcare and environment.chiral conversion. Bailey J. Effect of pH and temperature on enzyme activity. Estimation of Michaelis Menten parameters. H.I: Introduction to research The hallmarks of scientific research – the building blocks of science in research – relevance to preliminary information. 1986 2. pharmaceutical and other industries.W.. recombinant material safety. Biochemical Engineering. Modeling of rate equations for single and multiple substrate reactions. quantification of enzyme activity and specific activity. calculation of Effectiveness Factors of immobilized enzyme systems. USA. n u r a y i n U s r e v y it BT313 RESEARCH METHODOLOGY Marks: (40+60) Credit: 4:0:0 UNIT. Ellis Harwood 1995.. esterification etc. Clark. Design of enzyme electrodes and case studies on their application as biosensors in industry. D. Blanch. enzymes for diagnostic applications. D. Unit –II Immobilised enzyme reactions Techniques of enzyme immobilisation-matrix entrapment. Laboratory safety. ionic and cross linking. Case studies on application . Analysis of mass transfer effects of kinetics of immobilised enzyme reactions. & Ollis.. column packing. Marcel Dekker. Commercial applications of enzymes in food.

UNIT. Wishva Prakashan. bookkeeping. 2. Research Methodology.R. Thomson Asia Pvt. 2000. Probability analysis – Testing of significance – Goodness of fit (X2 test) – Student’s – test – Simple & multiple regression – Correlation: Canonical Correlation – Correlation coefficient– ANOVA (one way and two way analysis). Cooper and remela S. Sage Publications. the introductory section. Ranjit Kumar. Measures of central tendency. method section. John Wiley and Sons Inc. Donald R. Research methodology. rules and regulation for animal and human experiments. Veal. Ticehurst and A. Ltd. Sampling techniques. 1999. Review of the advantages and disadvantages of various data collection methods and when to use each. Dispersion : Skewness and Kurtosis. Guidelines for questionnaire design – electronic questionnaire design and surveys. Doing Management research. UNIT. Donald H. Types of biological data – Collection and presentation of data (Table. Raymond – Alain Thie’ tart. Data relevance to intellectual property rights (IPR). Factor analysis – Cluster analysis – Discrimnant analysis. References: 1. 1999. Text Books: 1. 2002 2.V: The Research Report The purpose of the written report – concept of audience – Basics of written reports. Uma Sekaran. New Delhi. Research Methods for Business. Developing scales: rating scale and attitudinal scales. Hypothesis testing. Business research methods. London. In vitro. New Delhi. New York. Application of SPSS package.W. static and dynamic panels. research methods. Diagrams). Sage publications. Issues of precision and confidence in determining sample size. The integral parts of a report – the little of a report. results section – discussion section – recommendations and implementation section and reference section. G. 2001. Singapore.. New Delhi. Measurement of variables – scales and measurements of variables.III: Data collection methods Interviewing questionnaires etc. UNIT.II: Experimental designs The laboratory and the field experiment – internal and external validity – factors affecting internal validity. Reliability concept in the scales being developed. Longman. a K n u r a y i n U s r e v y it School of Biotechnology AN-188 . Probabilistic and non – probabilistic samples.. McBurney. et. Graphs. London. Stability measures. in vivo and clinical trial designs. Business Research Methods. 3. the table of contents. Schindler. Al. C. the synopsis. Determination of optimal sample size. 3. Kothari. Tata McGraw Hill publishing company limited. secondary sources of data collection. Validity testing of scales developed. UNIT. Special date sources: focus groups. 4. 1999. 2000.IV: Biostatistics Definition and scope.J.

Press. Hall & A. bioinformatics and drug discovery. Immunoelectrophoresis. Second Edition. Literature search. Unit-V Bioinformatics Biological data base-nucleotide. Whitaker. Jerrold H.Zar (1999) Biostatistical analysis by. Immunoblotting. scintillation counter. Electron (Scanning and Transmission) –Radio isotopic techniques-X ray diffraction. Nucleic acid isolation – Isolation of genomic DNA. and Affinity column. Stephen J. Text Books: 1. Packed bed. alignment. PCR and. Phase contrast. research and scientific methods. FASTA: Sequence comparision. Peter F. Isoelectrofoccusing: Unit-III Structural Elucidation and Analysis Principles and applications of the following: Microscope – Flourescence. Butterworth – Heinemann An Imprint of Elsevier India Pvt. Plasmid DNA.(2002). Culture of microbes. Defining the research problem. Confocal. Bioprocess Engineering: Operation types: Continuous. research methods versus methodology. Monographs. Autoradiography. protein-structural and sequencial data base. CD.. Pulsed field electrophoresis. Blotting techniques and Hybridization techniques. GLC: GCMS. London. Centrifugation high speed and ultra. Electrophoresis – Polyacrylamide. IR. Modes of sterilization. Stanbury. Introduction to Bioinformatics. Atomic absorbtion spectrophotometer – Spectrofluorimetry. Prentice Hall International.J. Principles of Fermentation Technology. DSC. size exclusion. Spectrophotometry – Principle and application of Spectrophotometer – Visible. Plant and animal cell and Tissue. Molecular visualization tools study protein structure. HPLC and Gas chromatography. Citation literature. T. 2005 a K n u r a y i n U s r e v y it School of Biotechnology AN-189 . Ltd. Gene and protein expression data. 2. Attwood. Immobilization of enzyme and cells. Unit-IV Cell Culture and Molecular Biological Techniques Preparation of Culture media. Unit-II Separation and Analysis Principles of Chromatography: lon exchange. Sequence analysis-BLAST. Flowcytometry. RNA. Use of microarrays to study gene expression and protein expression. NMR. Inc. Fed batch. UV. GM counter. Significance of research. RIA. Singapore. Characterization of chromosomes by various banding techniques. Bibliography.K and Parry-Smith D. Research article. basic concepts in computer operations. Immunotechniques – ELISA. research design.BT314 INSTRUMENTATION AND BIOTECHNIQUES Credit: 4:0:0 Marks: (40+60) Unit-I Introduction to Methodology Format of thesis and dissertation. building phylogenetic trees. Designs: Fluidized bed. Reviews. Pearson education. Agarose. Internet resources. Batch. 3. Denaturing gradient gel electrophoresis.

K Razdan (2003). molecular biological techniques for rapid diagnosis of genetic diseases Unit V: Applications of animal biotechnology . Retrovirus vectors. Regulatory and safety aspects of GMOs. S. Ti plasmid and vectors. Methods and techniques. GMFs. Oxford and IBH Publishing Co.References: 1.Production of biopharmaceuticals. London. nuclear transfer for mammalian cells.K. Covey Blackie.Techniques for the insertion of foreign genes into plant cells.product safety. M. Jones (1990)..Publishers. transposons. Transgenics – Ethical concerns and solutions . New Delhi.G.S. 4. Lindsey and M. in vitro fertilization and embryo transfer. BT315 PLANT AND ANIMAL BIOTECHNOLOGY FOR FOOD ENGINEERS Credit :3:1:0 Unit I: Introduction Plant and Animal Biotechnology . production of transgenic plants. New Delhi.Techniques involved .Kothari.R. and insertional mutagenesis – Transgenic plants as sources of modified oils Unit IV: Basic techniques of animal cell culture and their application – Introduction of novel genes – Transfection. Plant biotechnology In Agriculture: K. 3. culture and fusion.Plant cell cultures for the production of food-related chemicals: pigments perfumes. plenum press Plant Molecular biology: D. New age International(P)Ltd. 2. Bhojwani (1990). Elsevier.E. Animal cell Biotechnology. Amsterdam.B Griffiths. C.Pvt. Prentice hall. Academic Press. Research Methodology. (i) Transgenic plants (ii) gene cloning. xenotransplantation.N. Lever.Protoplast isolation. New Jersey.Ltd. flavors Unit III: Genetic Engineering . Grierson & S.J. transgenic animals. Plant Tissue Culture.Importance in Bio protection and conservation Unit II: Plant cell and tissue culture . public perception of Biotechnology – Legislation. Plant Tissue Culture: Applications and Limitations. Spier and J. IInd edition (2004). Genome Organization and Expression in Plants: C. Gene therapy – Basics and application to cancers and Tumours – Therapeutic applications of antisense nucleic acids Text Books: 1. R. restriction fragment length polymorphisms. Properties and applications of Hairy root cultures – Bioreactors for Plant cell and Tissue cultures . 2. (1998) School of Biotechnology AN-190 a K n u r a y i n U s r e v Marks: 40+60 y it .

Butterworth – Heinemann An Imprint of Elsevier India Pvt. John Wiley And Sons.. Marcel Dekker Inc. mixed or sequential bioconversions. 1980.cAMP deficiency. alteration of permeability for metabolites. Madras. Demain A.P. feedback regulation. Animal cells . 6. CRC Press. Stanbury.C.. induction.. Cooney C. a. limiting accumulation of end products. Plant Biotechnology and Transgenic Plants. Whitaker. catabolite regulation by passing control of secondary metabolism. specificity. conversion of insoluble substances.Doyle. Humphery A. regulation in branched pathways. Principles of Fermentation Technology.H. Second Edition.E. K. group transportation.L. Pvt. gene dosage.D. and Holland A Animal Biotechnology and Ethics . Cambridge University Press. R. Ltd. feedback resistant mutants. Hui. 2002. 1989. feedback repression. yields. Wang D. catabolite regulationglucose effect.. 1998. Unit II: Synthesis Of Primary Metabolites Alteration of feed back regulation.. World Scientific Publishing Co. permeability control: passive diffusion. 8. 2005 References 1.Hay and B. Dashek W.. Kirsop (1990).. Unit III: Biosynthesis Of Secondary Metabolites producers of secondary metabolites.I. Zubay G. Ltd. Chapman and Hall. cumulative feed back regulation. Stephen J. Methods in Plant Biochemistry and Molecular Biology..V. mutation. Gene therapy – From Laboratory to the Clinic. " Biochemistry ". recognising growth cycle peak. active transport. Peter F. 1997.L. trophophase.M. idiophase relationship. permeability. Living resources for Biotechnology. mutants resistant to repression. Text books 1.. Unit IV: Bioconversions Advantages of bioconversions. BT316 METABOLIC ENGINEERING Credit : 3:1:0 Unit I: Introduction Jacob Monod model for gene expression regulation-Lac operon. improving fermentation. 2. catabolite repression. co-metabolism. " Fermentation And Enzyme Technology ". Singapore. regulation of enzyme synthesis. enzyme induction. Macmillan Publishers. avoidance of product inhibition. Dunnil. Hall & A.E. School of Biotechnology AN-191 n u r a y i n U s r e v y it Marks: 40+60 .differential regulation by isoenzymes. factors important to bioconversion. Cambridge. Lilly M. Feed back regulation. a K Unit V: Regulation Of Enzyme Production Strain selection. 9. Johnson A. Precursor effects. Regulation of RNA synthesis by amino acid.5. Kirsi-Marja Oksman-Caldentey and Barz W. concerted feed back regulation. 1994.. 7.

hydrophobic interaction. IMAC and bio-affinity chromatography. Bioseparation – Downstream processing for Biotechnology. formulation strategies. (1988) 2. Unit III: Isolation of products .S. conditioning.moving bed. Belter.criteria and methods for quality control of therapeutic proteins and recombinant proteins. Unit IV: Product purification . and Hudson M J Separations for Biotechnology. Scopes Protein purification : Principles and practive.Characterisation of biomolecules and fermentation broths. J Asenjo (ed) separation Processes in biotechnology. product characterisation and conditioning . Biotechnical series. ion-exchange. M. 3rd Edn.A. adsorption processes. Verrall. broad strategies for design of bioseparation processes. ultrafiltration. drying and lyophilisation. Taylor and Francis (2002) a K n u r a y i n U s r e v y it School of Biotechnology AN-192 .Crystallisation. aqueous two – phase extraction of proteins. design and selection of chromatographic matrices. design of large scale chromatographic separation processes. Biochromatography : Theory & Practice. Membrane processes – dialysis. M. reverse osmosis and electrodialysis.A. cake filtration and microfiltration. expanded bed. Butterworth Heinmann (1992) 3. Unit II: Cell disruption. Marcel-Dekker (1993) 4.Chromatography – principles of chromatographic separations – gel filtration.Solvent extraction of small molecules. Paul. Unit V: Final product purification and preparation . solid – liquid and size – based separations .BT317 DOWN STREAM PROCESSING Credit : 3:1:0 Marks (40+60) Unit I: Introduction to bioseparation .. alternative procedures . E. centrifugation and sedimentation. Robert K. Springer Verlag. (1994) 6. Product recovery in Bioprocess Technology Biotechnol Series.Wiley Inter Science Pub. electrophoretic separation processes. precipitation of proteins with salts and with organic solvents. reversed phase. Ellis Horwood Ltd. modes of operation. Vijayalakshmi.L.Cell disruption-mechanical and chemical methods. (1990) 5. roles of downstream processing in biotechnology. Text Books: 1. Cussler and Wei shan Hu.

spices. vegetables. vitamins etc.Dosages of radiation for various plant foods and animal foods-meat and poultry. G value. Unit II Radiation Chemistry Of Food Components Basics-carbohydrates. ionization and excitation. School of Biotechnology AN-193 n u r FP217 RADIATION PRESERVATION AND PROCESSING Marks 40+60 a y i n U s r e v y it . radiations for treating foods. dairy products. temperature. Radiation equipment. definitions. role of irradiation parameters. lipids.ADDITIONAL SUBJECTS Code FP217 FP218 FP219 FP220 FP221 FP222 FP223 FP301 FP302 FP303 FP304 FP305 FP306 FP307 FP308 FP309 FP310 FP311 FP312 FP313 FP314 FP315 FP316 FP317 Subject Radiation Preservation and Processing Processing of Foods Lab . Concept of radiation. primary chemical effects and secondary effects on good. dosage dose rate. quantitative relationships . Packaging of irradiated foods and safety issues.Radiation chemistry basics. yeasts and molds . additives. post irradiation effects. times for various radiation events. water content. Radiation effect on contaminating microorganisms like bacteria.2 Food Engineering and Packaging Lab Analysis of Food Commodities Lab – 2 Protein Chemistry And Engineering For Food engineers Food Refrigeration Engineering and Technology Principles of Food Processing Principles of Instrumental Analysis for Food Engineers Food chemistry Food Technology – I Food additives Food Engineering – I Food Engineering – II Food Technology – II Non-thermal methods of Food preservation Food Packaging Technology Analysis of Food Constituents Lab Enzymology lab Food and Chemical Engineering Lab Food Processing and Packaging Lab Protein Chemistry and Engineering for Food Engineers Design and Analysis of Heat Transfer Equipments Spray Drying: Application in the Food Industries Analysis of Food Commodities Lab Credit 4:0:0 0:0:2 0:0:2 0:0:2 3:1:0 4:0:0 4:0:0 3:1:0 3:1:0 3:1:0 3:1:0 3:1:0 3:1:0 3:1:0 3:1:0 3:1:0 0:0:2 0:0:2 0:0:2 0:0:2 3:1:0 3:1:0 3:1:0 0:0:2 Credit: 4:0:0 a K Unit I Basics of Radiation Chemistry Ionizing Radiations. viruses. salient features. interaction of ionizing radiation with food matter. fruits. proteins.

RF heating equipment including conveyorized dryers . pasteurization/ sterilization and processing of meat products. nature of energy. New Product Development Food & Nutrition Press Inc. New Methods of Food Preservation. UK (2002) 4. Urbain: Food Irradiation Academic Press. Wilkinson Guild: Food Irradiation – A Reference Guide Woodhead Publishing.Relative merits and demerits of IR radiation over other radiations Unit V Radio Frequency Heating Principles Advantages of Radio frequency heating of foods. Krasnikov: Physical Principles of Infra Red Irradiation of Food Stuffs: Hemisphere Publishing Corporation.Ultra violet radiation and its effect on microorganisms in foods . microwave generators.G. Polarization characteristics of IR radiation. batch and continuous ovens. NY (1995) 2.Unit III Microwaves In Food Processing Electronic heating terminology. Radiation equipment. Philip Richardson: Thermal Technologies for Food Processing Woodhead Publishing Limited. Cambridge. Applications in dehydration. Ginzburg: Applications of Infra-Red Radiation in Food Processing Leonord Hill.some applications in food technology . S. Ohlsson and Bengtson: Microwave Processing Technologies Woodhead Publishing. blanching.V. CRC Press.Llyasor and V. Welter M. brief description of oven construction. Decareau: Microwaves in Food Processing Industry Academic Press inc.2 Marks 50+50 12 experiments will be notified by the HOD from time to time Credit: 0:0:2 School of Biotechnology AN-194 . Aspen Publisheres Inc. New York (1985) 3.S. Robert V. London (1991) 5. London (1969) a K n u r a y i n U s r e v y it FP218 PROCESSING OF FOODS LAB .Diehl: Safety of Radiated Foods Marcel Dekker Inc. USA (1992) 7. microwave energy. Instruments for measuring radiation . thawing. Maryland. Robert V. A. Industrial application of microwave radiation. IR generators. conveyorized ovens. (2001) 6.Propagation of IR radiation in food stuffs. 1999. J. New York (1986) 2.UV treatment application and equipment Text Books 1. Gould G. Unit IV Infra red Radiation Absorption and scattering characteristics of various food materials. wave guides.F.W. Decareau: Microwave Foods. baking. UK (1996) 3. Cambridge. Reference Books 1. dielectric properties and radiation.

recombinant insulin to reduce aggregation and inactivation. imidazole groups) and peptide synthesis. formation of complexes. infrared. beta-sheets.2 Credit: 0:0:2 12 experiments will be notified by the HOD from time to time FP221 PROTEIN CHEMISTRY AND ENGINEERING FOR FOOD ENGINEERS Credit : 3:1:0 UNIT I Basics of amino acids and protein chemistry Amino acids (the students should be thorough with three and single letter codes) and their molecular properties (size. thiol. alphabeta-alpha.automated Edman method & mass-spec. a K UNIT III Protein Engineering Advantages and purpose. ultraviolet. X-ray) and elucidation of protein structure. Coordinate. Interaction with electromagnetic radiation (radio. overview of methods. Quaternary structure: Modular nature. hydroxyl. hydrophobic and Vander walls interactions in protein structure. charge. de novo protein design. folding. Covalent. carboxyl. pKa). peptide sequencing . Hydrogen.FP219 FOOD ENGINEERING AND PACKAGING LAB Credit: 0:0:2 12 experiments will be notified by the HOD from time to time Marks 50+50 FP220 ANALYSIS OF FOOD COMMODITIES LAB . underlying principles with specific examples: thermal stability T4-lysozyme. overview of methods to determine 3D structures. micro. UNIT II Protein Architecture Primary structure: peptide mapping. topology diagrams. solubility. beta and loop structures and methods to determine Super-secondary structure: Alpha-turn-alpha. up and down & TIM barrel structures nucleotide binding folds. visible. prediction of substrate binding sites Tertiary structure: Domains. beta-turn-beta (hairpin). High-throughput protein sequencing setup Secondary structure: Alpha. denaturation and renaturation. Ionic. Chemical reactivity in relation to posttranslational modification (involving amino. UNIT IV Properties of Food proteins – Animal and Marine sources Caseins and Whey proteins Caseins – Heterogeneity and Molecular properties – caseins micelles – Mechanism of stabilization – Structure models – Structure of whey proteins and improvement of functionality Muscle proteins – Structure and functionality – Application of muscle proteins in foods School of Biotechnology AN-195 n u r a y i n U s r e v Marks 50+50 y it Marks (40+60) .

and Wilkinson A.E. NY. Third Edn. Stacking and handling of material in and around cold rooms. Cold Room temperatures. 1993 2.E. Atmospheric air and its properties.. 1999 FP222 FOOD REFRIGERATION ENGINEERING AND TECHNOLOGY Credit : 4:0:0 Unit I Principles of Refrigeration Refrigeration cycles. F.C. 4. Insulation. marine products. 2001 2. fruits and vegetables. 1982. Properties and functional properties of soya. Second Edition. Fox. Leaf as a protein soure – Basic and Food applications of rubisco Text Books 1. insulation of freezer rooms: Pre-cooling and pre freezing. Freeman WH. Refrigerants. defrosting practices. Second Edition”. condenser. UK. walk-in-coolers. air diffusion equipment. Green house Potential Refrigerants. Oxford. digestibility of algal proteins – applications to food systems UNIT V Properties of Food proteins – Plant sources Compostion.. Reference Books 1. USA. Ozone Depletion Potentials. preventive maintenance. Expansion valves piping and different controls. “Protein Engineering”. “Biochemistry”. Chemical and Functional Properties of Food Proteins. Kluwer Science. Evaporator. peanut. Moody P. Optimum temperatures of storage for different food materials-meat and poultry products. New and Developing Sources of Food Proteins. ton of refrigeration Components of a Refrigeration system: Compressor. Chapman and Hall. Cleanliness. safety measures Controlled atmosphere and Modified atmosphere storages Principles and basics of their construction School of Biotechnology AN-196 a K n u r a y i n U s r e v y it Marks 40+60 . and Tooze J. Creighton T. 1994. 5. Proteins. CRC aw Press. Garland Publishing. 2001. B. P. J. J. IRL Press. rapeseed. “Introduction to Protein Structured.J. use of non polluting refrigerants. Zidzisł E. J. Freezer room Temperatures. John Wiley and Sons.Sea weed proteins – Protein content and functionality. Voet D. prefabricated systems. Food Proteins. Hudson. properties of insulating materials. Cold Storage practice. F. Energy considerations Unit IICold Storage Design and Construction Small and large commercial storages. Vapour Compression and Vapour Absorption cycles. Branden C. and Refrigerated container trucks: Freezer Storages. Sikorski. spices and food grains Unit III Effect of cold storing on quality of certain food products Operation and maintenance. 1999 3. characteristics of different refrigerants. Cold load estimation. and Voet G. net refrigerating effect. Psychometrics. Condon. Doors and other openings.

1&2) AVI Publishing Company Inc. growth rate of ice crystals. meat and poultry. Colin Dennis and Michael Stringer: Chilled Foods – A Comprehensive Guide Ellis Horwood Publishing. Freezing rates. Mumbai (1999) a K Credit : 4:0:0 UNIT I : Fundamentals of Food Chemistry Carbohydrates – Classification – Simple & complex.Evers: The Freezing Preservation of Foods (Vol. Publishers. Domkundwar and Subhash Arora: A Course in refrigeration and Air Conditioning: Dhanpat Rai and sons.Mallet: Frozen Food Technology Balckie Academic and Professional. New york (1992) 9. di-. Iodine value. USA 1957 2.Dellino: Cold and Chilled Storage Technology Publisher: Kluwer Academic Publishers (1997) 3. J. New Delhi (1994) 4.Gunther: Refrigeration. Freezer types. Acid value. 1982 5.Hygienic design considerations for chillers and chilled Storages.K. Secondary refrigerants and direct expansion techniques in chilling. Ed. S. Air conditioning and Cold Storage Chilten Company. Blast freezers. E.Pruthi: Quick Freezing Preservation of Foods (2 Volumes) Allied Publishers. fruits and vegetables. mono-. Evaporative cooling and its applications Unit V Freezing of foods Freezing equipment. (1993) 7.F. Andrew D Althouse and others: Refrigeration and air Conditioning Goodheart – Willcox Company Inc.Tressler and C. Chilled foods transport and display cabinets .P. conveyorized quick freezers. (1980) 6. Important reaction of carbohydrates –Caramelisation. Acetyl value. Aurel Gobaneu and Gabriala Laseha and others (1976) Cooling Technology in the Food Industry: Abacus Press. Text/Reference Books 1.R. Freezing practice as applied to marine foods. Raymond R. Cryogenic Freezing. crystal size and its effect of texture and quality of foods. Clive D. Cool Storages and their applications. oligo.and polysaccharides. Maillard Fats – classification – Analysis of Fats and oils – Saponifcation value. USA (1965) 10. Individual quick freezing.J. U.Basics of Chilled foods microbiology.Hollowell: Cold Storage and Freezer Storage Manual AVI Publishing Co. Tunbridge Wells. Peroxide value – Principles and Importance of the analytical methods Vitamins – Fat and water-soluble – nutritional significance n u r a y i n U s r e v y it FP223 PRINCIPLES OF FOOD PROCESSING Marks (40+60) School of Biotechnology AN-197 . Philadelphia. Packaging of Chilled foods .S. D.Unit IV Chilling of Foods Chilling equipment for liquid foods.K. C. 8. Contact Plate Freezers.

Royal Society. Prentice-Hall Of India Pvt. Immobilized metal affinity chromatography – Ion chromatography.Classification. 2 Edn. DNA sequencing. DNA and Protein Analysis. spoilage of vegetable.Classical chromatography. 1987. References th 1. 2. frozen storagefreezing methods.DNA purification. 4 Ed. factors affecting quality of frozen foods. enzymes as food processing aids. Frazier And D. Ltd. New Delhi 2002. New York 1988. Text Books nd 1. Supercritical Fluid chromatography School of Biotechnology AN-198 n u r a y i n U s r e v y it Marks: 40 + 60 . Z and F values – Importance of 12 D concept. Unit II: Chromatography . beverage and other food products. UNIT III : Microorganisms Associated With Food Bacteria. UNIT V : Food Preservation Principles involved in the use of sterilization.Gas chromatography. 1992. food colourants – natural and artificial. Westhoff – Food Microbiology. intentional and non-intentional additives. W. food flavours. methods of determination of thermal death time (Graphical.C. types and species of importance in food processing and preservation. food intoxications and poisonings – bacterial and non-bacterial.. localization of cells in tissues immunoblotting. Immunofluroscence. ion exchange chromatography. Jay – Modern Food Microbiology. adsorption. fermented foods and food chemicals. Sivasanker – Food Processing And Preservation. fruit.P.C.Canning.UNIT II : Food Additives Classification. poultry. B. 2. irradiation preservation of foods. pasteurization and blanching. meat. New Delhi.Antiserum production. London.HPLC. Immuno-techniques . food spoilage – factors responsible for spoilage. immunohistocompatibility ELISA. J. PCR-based analysis. CBS Pub. functional role in food processing and preservation. UNIT IV : Food Borne Diseases Classification – food infections – bacterial and other types. DNA finger printing. yeasts and molds – sources. FP301 PRINCIPLES OF INSTRUMENTAL ANALYSIS FOR FOOD ENGINEERS a K Credit : 3:1:0 Unit I: Nucleotide. mathematical) – D. T. monoclonal antibodies.. Coultate – Food – The Chemistry Of Its Components. single cell protein. Time – Temperature indicators . McGraw-Hill Book Co.M.

Unit III: Spectroscopy – NMR – Liquid and solid. Atomic absorption spectroscopy Unit V: Non-destructive methods of analysis of Food Commodities Text books: rd 1. D. Ewing GW. IR. School of Biotechnology AN-199 a K n u r a y i n U s r e v y it Marks : 40 + 60 . and unsaponifiables. identification & purity of Proteins. Components of Fatty acids.Process flow sheet for the manufacture of edible oils (refined and hydrogenated) Unit III: Chemistry of Proteins: Importance of Proteins. Physical & chemical characteristics of fats & oils. Chemical reactions of carbohydrates. classification. Rancidity of fats & oils. peptides & Proteins.Chemistry. 2000.Dispersed systems – some basic considerations . cocoa butter substitutes and equivalents. Denaturation. Physical & chemical characteristics of Proteins. England. Starch and its hydrolytic products. Cellulose. H. Braun. Chemistry of fatty acids & glycerides. Protein based emulsions and foams. Spectrophotometer. and Rouessac A. gums & polysaccharides. 3 Edition. 1987. McGraw Hill. Nomenclature.Structure activity relationship related to taste Chemistry of Carbohydrates: Nomenclature Classification & structure of carbohydrates. properties and preparation of Pectic substances. Basic Structures. antioxidants. FP302 FOOD CHEMISTRY Credit : 3:1:0 Unit I: Water and ice: Importance of water in foods. H. Introduction to Chemical Analysis. 2. Ltd. Peck. 1989. shortening agents. 3. Concept of bound & free water & their implications . Chemical Analysis: Modern Instrumentation Methods and Techniques. Sources and distribution of Proteins. Auto oxidation and hydrolysis. Phospolipids. Physical & chemical properties of sugars . Mc Graw Hill Book Company. and its prevention. maltodextrins. Cyclodextrins Unit II: Chemistry of lipids Definition & classification of lipids. Fluorimetry Unit IV: Colorimetry and Spectrometry . Holme. Rouessac F. Isolation. Willard and Merrit Instrumental Methods and Analysis. confectionary fats. structure and chemistry of amino acids.Radio active techniques. FTIR. John Wiley and Sons. 2. Analytical Biochemistry. Reference Books: 1. Structure of water & ice. Longman Publications. VI Edition CBS Publishers & Distributors. Instrumental Methods of Chemical Analysis.

Assay Techniques. W Grosch and P Schieberle: Food Chemistry . M : Biochemistry Of Foods Harcourt Publishers Ltd.Coulate: Food – Chemistry of its Components Royal Society of Chemistry. Sauce manufacture Minimal processing of Fruits and Vegetables – MAP and CAP – High Pressure processing – Edible coatings – Osmotic dehydration – Fruit and Vegetable dehydrations – Fruit and Vegetable powders – Quality control Unit II: Cereals and Pulses Technology Milling of Cereals – Rice. Pieter Walstra: Physical Chemistry of Foods Marcel Dekker Publishing. West port.3rd Edition (2004) Springer Verlag March 2004 3.Robinson: Food Biochemistry and Nutritional Value Longman Scientific and Technical Publishers. H-D Belitz. Lipoxygenases. David S. Maize. USA (1997) 6. Eskin. T. USA (Edition #2.B. Immobilized enzymeOne example of working of each enzyme.P. New York (2003) 5.Breverman: Introduction to Biochemistry of Foods Elsevier Publishing Company. N. USA (1984) a K Credit : 3:1:0 n u r a y i n U s r e v y it FP303 FOOD TECHNOLOGY – I Marks : 40 + 60 Unit I: Technology of Fruit and Vegetable Processing .1 Climacteric and Non-climacteric fruits – Overview of processing technologies including pickling. Oxidoreductases.Unit IV: Enzymes: Introduction. 1990) 2. Sikorski: Chemical and Functional Properties of Food Components Technomic Publishing. Nature.Phenolases. Proteases. Catalases. Wheat. With Henderson. H. New York (1996). Xanthine Oxidases. RTS beverages. Amylases. Barley School of Biotechnology AN-200 . John M Demann: Principles of Food Chemistry AVI Publishing Co. Zd Zislaw and E. Glucose Oxidases. USA 1997 Reference Books: 1. USA 1974 4. Specificity. Owen R Fennema : Food Chemistry – III edition Marcel Dekkar Inc. classification & nomenclature of enzymes. 2.S. Jam. USA (1987) 3. Oats. Juice. USA (1963) 4. Lancaster. Zdzislaw E. and Concentrates. Pectic Enzymes. A. Sorghum. Peroxidases. carnitine Text Books: 1. J.Sikroski: Chemical and functional Properties of Food Components: Technomic Publishing company. Isolation & purification of enzymes & their importance Unit V: Chemistry of Vitamins: Summary of vitamin stability – Toxicity and sources of vitamins – Bioavailability of vitamins – Reasons for the loss of vitamins in foods – Fat-soluble and water soluble vitamins – Choline. Function.

Chocolate. teratogenicity – PFA. Coffee and Cocoa (Chemistry and Technology) –– Process for manufacture of CTC and Green tea. Aspen Publication. Instant tea. Great Britain. Aspen Publication. Second edition. Cereal and Cereal Products – Technology and Chemistry (Food Products series).Quality control Unit IV: Technology of Plantation products: Tea.Parboiling of rice – Products from rice – Convenience food products from rice Maize – Processing of maize – Tortillas Quality control Unit III: Baking and Extrusion technology Manufacture of Baked products (Chemistry and Technology) – Process of bread manufacture –Cake manufacture – Pastries – Recent developments .A.Cocoa processing – Fermentation. Aspen publishers Inc. Aspen publishers Inc. Jackson: Sugar Confectioney Manufacture. toxicity data of Acidulants – Preservatives – Emulsifiers and gums .M. carcinogenicity.1999.A. a K Credit : 3:1:0 Unit I : Introduction – food additives definition – Determination of the limit for addition – NOEL – Toxicity data – Method of determining toxicity – LD50. and Dobraszczyk B. Instant coffee . FDA.Extrusion of cereal flours – Pasta and Noodle manufacture . III Edition. 5. levels of additions in individual products. USA (2001)... Chemistry and Microbiology. Manufacture of cocoa powder. chemical properties. E. Beverages : Technology. Great Britain. Blackie and Son Ltd.. Newyork. 1999.. Street C. 4. chocolates Unit V: Chemistry and Technology of sugar products and beverages – Alcoholic and Non-alcoholic Sugar – Process for the manufacture of sugar – Grading of sugar – Quality control aspects Process for the manufacture of confectionery products . Minifie Bernard W.J. 1991. FPO regulations – GRAS additives Unit II: Types. and Sutherland J. Cocoa and Confectionery Technology.Manufacture of carbonated beverages – Recent developments .B.Chemistry and Microbiology of Beer and Wine Manufacture of distilled spirits Text books: 1.Antioxidants n u r a y i n U s r e v y it FP304 FOOD ADDITIVES Marks : 40 + 60 School of Biotechnology AN-201 . VCH Publishers Inc. 3. Dendy D. Varnam A. Flour Confectionery Manufacture. 2.1999.

Pitot tube. Nusselt number. Peclet number. Examples Industrial applications and equipments for Filtration and Sedimentation . and Thorngate J.Dimensionless ratios and calculations involving various physical parameters . Disk bowl.Specific characteristics. chemical properties. FP305 FOOD ENGINEERING . Starches Unit IV: Types. Unit IV: Membrane Filtration: Definitions. Second Edition. Laminar and Turbulent flow. Venturimeter. Newtonian and non Newtonian fluids. Tubular Bowl. Handbook of Food additives by Thomas Furia. Units and dimensions of engineering parameters . antibrowning agents. Ultra filtration (UF) and Micro filtration (MF). friction losses in pipes valves and bends. Food additives by Brannen A. levels of additions in individual products. Importance of balancing of rotating masses. Reverse Osmosis (RO). chemical properties. relative advantages and limitations. Fat substitutes and replacers Unit V: Types. toxicity data of Colourants – Natural and artificial. Salminen S. Decanting. Molecular weight cut off in each case.. 2. School of Biotechnology AN-202 n u r a y i n U s r e v y it Marks : 40 + 60 . the Notch or weir. Marcel dekker Inc.I Credit : 3:1:0 Unit I: Introduction: Basic principles of food processing.H. Pumps and other membrane equipment..flour improvers – Humectants –Enzymes.theorem Unit II: Fluid flow theory and applications: Fluid statics. feed and discharge arrangement in each case . levels of additions in individual products. Configuration of membranes. hot wire anemometer a K Unit III: Mechanical and Centrifugal separations. 2002. Nutritional additives Text book: 1. Chelating agents.Flow measuring devices – Orificemeter. USA. membrane materials. Desludging (Self Cleaning) Bowl Centrifuges. chemical properties. gravity sedimentation. Nozzle centrifuges. Membranes and their characteristics. Grashof number and Fourier number . Flavour enhancers. Davidson P. Rotameter. Nano filtration (NF).L. toxicity data Sweeteners – Natural and synthetic. Cross flow filtration. Revised and Expanded. the Nozzle. Simple calculations . Flavourants. Different types of centrifuges – Basket.Significance of Reynold’s number. Conservation of mass and energy.Dimensional analysis by Rayleigh’s method and Buckingham pi. pressure dynamics. toxicity data of Dough conditioners .Centrifuges.Unit III: Types. Bernoullie’s equation. Applications in food industry. levels of additions in individual products. Filtration and sedimentation.M. Basic equations. advantages and applications.

climbing film tubular evaporators. Hong Kong 2. Blackie Academic and Professional. Fellows : Food Processing Technology: Principles and Practice.L. Stahl and G.L. Second Edition. P. Keller: Separation Process Technology.Prediction of drying time . Vapour recompression. Mcgraw Hill New York 5. Cambridge UK Credit : 3:1:0 a K Unit I: Distillation and Adsorption: Distillation.S. 2002. Steam distillation. Woodhead Publishing Limited.M.Elsevier science Ltd. boiling point elevation. Coulson.Earle: Unit Operations in Food Processing. applications and equipment. Harker . London. Text Books 1. E. D. 2000. Diffusion of gases in liquid and solid foods Unit II: Evaporation: Needs. USA 1986 Reference Books 1. Heat economy.Lewis: Separation processes in the Food and Biotechnology industries (Principles and applications): Woodhead publishing Ltd. J. Humphry and G. Molecular distillation.Wilke: Extraction with Supercritical Gases.Schneider.Coulson & Richardson's Chemical Engineering. G.Dehydration systems – Tray or tunnel dryers – Spray driers. Marcel Dekker Inc. Elsevier Publishing Maynord Joslyn and J. thin film and scraped surface evaporators. A.Unit V: Roasting and Cooking: Theory. Richardson.Rizwi: Bio Separation Process in Foods. Mass transfer phenomenon applied to food systems . (II Edition or later). Single and multiple effect evaporation. Pyle and C.H. Unit III: Dehydration: Principle of dehydration – Theories of drying – Drying curves – Constant and falling rates of drying of foods . 2. Food Processing Operations (3 Volumes) AVI Publishing company Inc. New York 3. Calculations. Jimmy L. Verlag Chemie publishing. Fifth Edition. plate evaporators. Brennen: Food Engineering Operations (Vol 1 and 2). Advantages of Extrusion cooking over conventional cooking.Vol 2 Particle Technology and Separation Processes.Heid: 5. R. theory and examples. Butterworth & Heinemann .J. Different kinds of Roasters. Fluidised bed driers.D. Basel 3. Rakesh Singh and S. Thermo and mechanical systems. J. Rielly: Chemical engineering for the Food Industr.Grandison and M. Fryer.L. basic principles. F.M. Freeze driers School of Biotechnology AN-203 n u r FP306 FOOD ENGINEERING-II Marks : 40 + 60 a y i n U s r e v y it .Basics of adsorption. Forming extruders and snack food extruders. E. 4. Steam Cookers. Falling film. J. Chennai 4. Single and Twin Screw Extruders.H. Pergamen Press. Extrusion cooking.J.F.

Pergamen Press. J.Lenigar and W. J. Applications.A. Solubility.M. 2. J. Marcus Karel & Deryl B Lund: Physical Principles of Food Processing.Direct and Indirect Contact freezing systems – Plank’s equation – Prediction of freezing time – Modelling thermal properties of frozen foods . D. Toned milks.H.A. Yoghurt.Unit IV: Contact equilibrium separation processes: Basics of Solid-liquid extraction processes. Boston. Markotte: Food Processing Principles and Applications.Earle: Unit Operations in Food Processing. Richardson. Butterworth & Heinemann .Thawing Text Books: 1. CRC Press 2. Whey products – Quality control School of Biotechnology AN-204 n u r a y i n U s r e v y it FP307 FOOD TECHNOLOGY – II Marks : 40 + 60 . AVI Publishing Company Inc. extraction of Fatty acids. CRC Press Ltd.S.Beverlo: Food Process Engineering. Hops. Dennis R. Mass transfer operations. Properties of Super critical CO2. Microbiology and Technology of Cheese. Viscosity. 2002. Zacharias B. Butter.Reidal Publishing Co. Harker . Relative advantages. USA 3. Volatility etc. liquid-liquid extraction extraction. with food application examples. Coulson.Elsevier science Ltd. 4. Crystallization Super critical fluid extraction: Super critical Fluid State. Maroulis and G.Manufacture of Icecreams and Frozen Desserts – Physical chemistry of Icecreams – Simple problems – Process for the manufacture of icecreams – Defects in Icecreams Unit II: Dairy Technology – II Chemistry. Pasteurisation – HTST. USA (2003) a K Credit : 3:1:0 Unit 1: Dairy Technology -1 Milk – Physical and Chemical properties of milk – Milk microbiology .Importance of constituents in maintaining emulsion characteristics – Processing of milk – Standardised. H.F.Experimental measurement of freezing time . (1981) 5. 3.Coulson & Richardson's Chemical Engineering. Heldman and R. Fifth Edition. Ramaswamy & M. New York Reference Books: 1. H. Paul singh: Food Process Engineering (II Edition). Process – Sterilisation of milk – Process .Vol 2 Particle Technology and Separation Processes. SCFE systems and components. Dahi.L. R. Density. (II Edition or later). limitations and economics Unit V: Food Freezing Basic principle – Factors influencing freezing of food products . D.Milk powder – Dairy whiteners . Marcel Dekker Inc. Saravacos: Food Process Design Marcel Dekker. Supercritical Phase Equilibria. Albert Ebarz: Unit Operations in food Processing. Essential oils and Bio chemicals.

2 Comminuted meat products – cured meats – Bacon and Ham – Luncheon meats .Eggs – Processing principles – Moisture retention – Fat retention – Meat binding – Curing – effects of curing ingredients – Colour and Flavour of mear – Microbiology Chilling and freezing – Cooking Unit IV: Technology of Meat. proteins. times for various radiation events. plant suspension cultures Text Books: 1. ginger and turmeric. Blackwell Science. primary chemical effects and secondary effects on good. FP308 NON-THERMAL METHODS OF FOOD PRESERVATION Credit : 3:1:0 Marks : 40 + 60 a K Unit I: Ionizing Radiations: Concept of radiation. and Ranken M. Woodhead Publishing Ltd. additives. CRC Press. Cinnamon.Quality control . Egg white . Milk and Dairy Product Technology (Food Science and Technology). 1998. quantitative relationships. Radiation equipment.Eggs – Processing – Liquid eggs. Food Products Press Inc. dosage dose rate. Mechanically separated meat. salient features. Cumin. Unit II: Radiation chemistry of food components Basics-carbohydrates. rd 2. Pasteurisation. 1995 3.. Radiation chemistry basics. lipids. interaction of ionizing radiation with food matter. G value. Poultry Products Technology. curing and Smoking – Irradiation . role of irradiation parameters.V. Handbook of Meat Product Technology. Fish and Poultry products . School of Biotechnology AN-205 n u r a y i n U s r e v y it .Components of meat and their properties – Lean meat. Garlic. Radiation effect on contaminating microorganisms like bacteria. post irradiation effects.D. fenugreek. water content. dairy products.Present trends in synthesis of volatiles – micro-organisms. 4. ionization and excitation. radiations for treating foods.J.K. 3 Edition. Mountney G. Ranken Michael D. Clove and Vanilla – Oleoresins and essential oils – Method of manufacture – Chemistry of the volatiles –Enzymatic synthesis of flavour identicals . fruits. CRC Press. NY. 2004. 2000.C. vegetables.Sausages Canning and Dehydration.Methods of analysis and quality control of Meat and egg products Unit V: Technology of Spices and condiments: Pepper. head meat. Edgar Spreer.. vitamins etc. Coriander. definitions. spices. Connective tissures and Offals . temperature.1 Chemistry and Nutritive Characteristics of Various meat . yeasts and molds. Packaging of irradiated foods and safety issues.Unit III: Technology of Meat Fish and Poultry products . Cardamom. U. Peter K. viruses. England. Handbook of herbs and spices. Dosages of radiation for various plant foods and animal foods-meat and poultry. and Parkhurst R.

IR generators. J. UV treatment application and equipment. Aerosol Cans.Unit III: Microwaves in food processing Electronic heating terminology. Propagation of IR radiation in food stuffs. conveyorized ovens. Ultra violet radiation and its effect on microorganisms in foods. Applications in dehydration. New York (1986) 2. NY (1995) 4. Relative merits and demerits. thawing.G. General Approach. Some applications in food technology. Three piece cans and Two piece cans. Temperature. Mechanical strength (Tension. Text Books: 1.F. accelerated storage studies: Vacuum and Inert Gas Packaging: Tests on packaging materials. Radiation equipment. Robert V. Effect of environmental factors on food quality and shelf life. microwave generators. Need for protective packaging. (2001) Credit : 3:1:0 a K Unit I: Introduction to food packaging: Protection of Food products as major role of food packaging: Functions of packaging. Wilkinson Guild: Food Irradiation – A Reference Guide Woodhead Publishing. Welter M. Unit IV: Infra red radiation Absorption and scattering characteristics of various food materials. S. Relative merits and demerits of IR radiation over other radiations. Moisture. Ohlsson and Bengtson: Microwave Processing Technologies Woodhead Publishing. Gas and water vapour transmission rates Unit II: Metal Cans as packaging. nature of energy. School of Biotechnology AN-206 n u r a y i n U s r e v y it FP309 FOOD PACKAGING TECHNOLOGY Marks : 40 + 60 . UK (1996) 3. microwave energy. notch and tearing strengths). mechanical forces and biological factors on quality of food. CRC Press. RF heating equipment including conveyorized dryers. Merits and demerits. Basics of Canning operations. analysis of storage requirement. dielectric properties and radiation. Philip Richardson: Thermal Technologies for Food Processing Woodhead Publishing Limited. baking. Polarization characteristics of IR radiation.Llyasor and V. pasteurization/ sterilization and processing of meat products. Lacquers and their use. UK (2002) 6. wave guides. effect of light. batch and continuous ovens. Krasnikov: Physical Principles of Infra Red Irradiation of Food Stuffs: Hemisphere Publishing Corporation. New York (1985) 5. Estimating the Shelf life requirement of food products for packaging. Oxygen. Decareau: Microwaves in Food Processing Industry Academic Press inc. Urbain: Food Irradiation Academic Press. London (1991) 7. Unit V: Radio frequency heating principles Advantages of Radio frequency heating of foods. Cambridge. Cambridge. Instruments for measuring Radiation.V. blanching. brief description of oven construction. Industrial application of microwave radiation.Diehl: Safety of Radiated Foods Marcel Dekker Inc.

Germany (2000) a K n u r a y i n U s r e v y it School of Biotechnology AN-207 . Design features and applications. Glass jars and Bottles in food packaging. Filling and sealing of Flexible plastic containers. (Editor): Food Packaging and Preservation Aspen Publication. 1995. Mathlouthi M. Text Books: 1.Pirenger and A.Rooney: Active Food Packaging : Blackey Academic and Professional. Unit V: Interaction of food material with packaging material. Form fill Seal equipment: Printing on packages. Description and applications. J. different types of caps and liners used. Unit IV: Filling and Sealing operations for various types of packages. Metal caps for bottles and jars – Crown corks. 3. Polyesters. Bar codes. Frank A. lug caps. Fibre Board and Corrugated Card Board packaging and their applications. Active packaging. Sterilization of bottles.D. Shirly V. Formation of Films and pouches. R. Injection moulding. Jaipur India 6.R. Madras. GmbH. applications. Pouch form fill seal machines: Rigid and Semi rigid plastic packaging –Fabrication methods in brief –Thermo forming. 4. New York Reference Books 1. Second Edition. Plastics used and their Specific applications. Cellulose. Moisture control. Seal types-Bead seals. H Graves and V. M. Blackie Academic Profesional. Poyvinylidene Chloride (PVDC . Lap Seals and Fin seals –Differences and advantages. Polyvinyl chloride. 2006. advantages and problems. CO2 and Oxygen scavenging.Diofan. Bottle and jar closures. Gordon L. Ixan and Saran). advantages and disadvantages – Polyethylene (LDPE and HDPE). London (1995) 5.L. Nutrition labeling and legislative requirements.G. R. hot bar sealing and impulse sealing – differences and relative advantages.Baver: Plastic Packaging Materials for Food Wiely VCH. Hot wire sealing. Can double seam. New Delhi (2004) 2.David. 1999. Paine and Heather Y. Unit III: Flexible films packaging: Relative merits and demerits.Principles and Practice. CRC Press. terminology.Paine: A Hand Book of Food Packaging Second Edition. Modified atmosphere packaging – principles. applications: Laminated Paper board Cartons. Robertson: Food Packaging. NIIR Board: Food Packaging Technology Handbook National Institute of Industrial Research. Closing and sealing of Rigid plastic containers.L. Copolymers their applications. USA (1996) 3. Co-extruded films and Laminates Their applications. Donald Downing: Complete Course in Canning (3 Volumes) CTI Publications inc.Carlson: Aseptic Processing and Packaging of Foods: CRC Press.Can closures. Blow moulding. Twist off lid and ROPP caps. O.Vangrade and Morgy Woodburn: Food Preservation and Safety Surabhi Publications. Filling (Volumetric and Gravimetric) and Sealing of pouches.. Polypropylene (PP). can seam formation and defects. Taylor and Francis Group. 2. Extrusion Extrusion Blow moulding etc.

4. charge. Chemical reactivity in relation to posttranslational modification (involving amino.T. carboxyl. Coordinate. R. Ionic.): Food Labelling Woodhead Publishing Inc. Aaron L. London (1993) FP310 ANALYSIS OF FOOD CONSTITUENTS LAB Credit : 0:0:2 12 experiments will be notified by the HOD from time to time FP311 ENZYMOLOGY LAB Credit : 0:0:2 12 experiments will be notified by the HOD from time to time FP312 FOOD AND CHEMICAL ENGINEERING LAB Credit : 0:0:2 12 experiments will be notified by the HOD from time to time Credit : 0:0:2 a K FP314 PROTEIN CHEMISTRY AND ENGINEERING FOR FOOD ENGINEERS Marks:40+60 n u r FP313 FOOD PROCESSING AND PACKAGING LAB Marks : 50 + 50 a y i n U s r e v y it Marks : 50 + 50 Marks : 50 + 50 Marks : 50 + 50 12 experiments will be notified by the HOD from time to time Credit : 3:1:0 Unit I: Basics of amino acids and protein chemistry Amino acids (the students should be thorough with three and single letter codes) and their molecular properties (size. USA (1989) 8. J Ralph Blanch Field (Ed. Paul Singh: Food and Storage Stability CRC Press. USA (1997) 6. Hydrogen. CRC Press (2000) 7. imidazole groups) and peptide synthesis .Taub.Parry: Principles and Applications of Modified Atmosphere Packaging of foods: Blackey Academic and Professional. CRC Press USA (2000) 5. R.Covalent. hydrophobic and Vander walls School of Biotechnology AN-208 . David Kilcast & Persis Subramaniam: The Stability and Shelf Life of Food Woodhead Publishing Limited. pKa). solubility. Irwin A. hydroxyl. thiol. Brody: Controlled/Modified Atmosphere/Vacuum Packaging of Foods Food And Nutrition Pres.

1999 a K n u r a y i n U s r e v y it School of Biotechnology AN-209 .C. Garland Publishing. IRL Press. digestibility of algal proteins – applications to food systems Unit V: Properties of Food proteins – Plant sources Compostion. J.automated Edman method & mass-spec.. 2001. 1999 3. underlying principles with specific examples: thermal stability T4-lysozyme. 1994. Freeman WH. prediction of substrate binding sites Tertiary structure: Domains.. Second Edition”. Moody P. Chapman and Hall. overview of methods to determine 3D structures. Interaction with electromagnetic radiation (radio. Unit III: Protein Engineering Advantages and purpose. J. “Protein Engineering”. denaturation and renaturation. “Biochemistry”. B.interactions in protein structure. topology diagrams. NY. 5. Chemical and Functional Properties of Food Proteins.E. and Tooze J. Unit IV: Properties of Food proteins – Animal and Marine sources Caseins and Whey proteins Caseins – Heterogeneity and Molecular properties – caseins micelles – Mechanism of stabilization – Structure models – Structure of whey proteins and improvement of functionality Muscle proteins – Structure and functionality – Application of muscle proteins in foods Sea weed proteins – Protein content and functionality.E. beta and loop structures and methods to determine Super-secondary structure: Alpha-turn-alpha. New and Developing Sources of Food Proteins. 1982. Second Edition. overview of methods. Oxford. F. de novo protein design. recombinant insulin to reduce aggregation and inactivation. Hudson. Creighton T. 1993 2. and Wilkinson A. Third Edn. aw CRC Press. F. beta-sheets. micro. formation of complexes. Voet D. visible. “Introduction to Protein Structured. Branden C. UK. Quaternary structure: Modular nature. Zidzisł E. Sikorski. and Voet G. folding. Unit II: Protein Architecture Primary structure: peptide mapping.Leaf as a protein soure – Basic and Food applications of rubisco Text Books: 1. beta-turn-beta (hairpin). peanut . High-throughput protein sequencing setup Secondary structure: Alpha. ultraviolet. Reference Books: 1. X-ray) and elucidation of protein structure. Food Proteins. Condon. up and down & TIM barrel structures nucleotide binding folds. J. USA. Properties and functional properties of soya. Kluwer Science. Fox. 2001 2.J. 4. infrared. rapeseed. peptide sequencing . Proteins. alphabeta-alpha. P. John Wiley and Sons.

Design of Reheater. surface condensers..parallel flow and counter flow . ‘Compact Heat Exchangers’. design.Air Pre-Heaters: Types of Air pre-heater. Nag. classification of heat exchangers.FP315 DESIGN AND ANALYSIS OF HEAT TRANSFER EQUIPMENTS Credit :3:1: 0 Marks: 40+60 Unit I: Introduction. Wlker G. Design of condensers for Thermal power plant and refrigeration circuits. pitch. A basic guide.Baffles in heat exchangers –types. and London A. tube bundle. Vacuum efficiency –Air leakage into the condenser-air removal-dry. “Heat exchangers” . Arithmetic mean temperature difference(AMTD). ‘Power Plant Engineering (Steam and Nuclear)’. P.Heat transfer in reaction vessels-Helical cooling coil. a K Text Books: 1. 1984.1996. W.. Super heat temperature control. McGraw Hill. School of Biotechnology AN-210 n u r a y i n U s r e v y it . Sadik Kakac and Hongtan Liu. fouling in heat exchangers-fouling effect calculations. 1965.P.K... Design of Evaporators for refrigeration circuits and Food plants. Unit II: Design of shell and tube heat exchangers: Design considerations. John Wiley and Sons Inc. humidifying tower Unit IV: Economiser-types.. 1980.Number of transfer unit (NTU). Temperature and humidity gradients in water Cooling tower.CRC press. recuperative and regenerative Unit V: Evaporators –classification-horizontal and vertical. McGraw Hill V Book Co. jacketed vessels. Single effect and multi effect evaporator criteria for selecting evaporator. Effectiveness in heat exchangers. A. and Ozisik. Shell types. Desuperheater-design.M.L. ‘Heat Exchanger Design’. Super Heater –Design.logarithmic mean temperature difference(LMTD)-parallel flow and counter flow.1998. super heater performance. time required for heating or cooling Unit III: Design of condensers – Economic selection of condensers. baffle spacing. 3 Ed. New Delhi.N. ‘Industrial Heat Exchangers’. 2. wet pumps. Tata McGraw Hill. overall heat transfer coefficient . Types-Direct contect. Pressure drop and pumping power calculations . Cooling tower-Types and design of natural and mechanical draught towers. Economy of super heat limit of super heat... 3. overall heat transfer coefficient. 2. Kays. M. Frass. steam mass flow gas mass flow and pressure drop in super heater. Reference Books: rd 1.

Representation of sprays. United States of America. (simple problems) Unit-V: Application in the food industryFood products and Plant extracts. Specification and design procedures for Open and Closed cycle (simple problems) Unit IV: Spray movement and drying of droplets in Spray Dryers Motion of single Droplet. terminal velocity and drag coefficient .Mean diameter.. “Liquid atomization”.. Process lay out-Open. L. and relative humidity. Closed. 1993.Evaporation of pure liquid droplet-evaporation rate.Bayvel. Z. Taylor and Francis. Unit II: Drying Principles Wet bulb. Evaporation time. Evaporation of droplets containing dissolved solids and insoluble solids. milk products-eggs. Size difference between product before and after drying-separation and recovery of dried products. Semi-closed and two stage. K. Third edition Reference Books: 1. fish industries. droplet deceleration from rotary and nozzle atomizers. Dry bulb temperatures. Heat and mass balance over spray dryers. New York.FP316 SPRAY DRYING: APPLICATION IN THE FOOD INDUSTRIES Credit : 3:1:0 Marks : 40 + 60 Unit I: Spray drying fundamentals Applications spray drying process stages.Masters . “Spray drying handbook” John Wiley and sons. Various types of atomizers used in spray drying industries-working principles. Enthalpy-Humidity chart application to Spray Drying. Thermal efficiency (simple problems) Unit III: Design of Spray Dryers Importance of residence time. fruits and vegetales. a K n u r a y i n U s r e v y it FP317 ANALYSIS OF FOOD COMMODITIES LAB Marks : 50 + 50 Credit : 0:0:2 12 experiments will be notified by the HOD from time to time School of Biotechnology AN-211 . Text Book: 1.Orzechowski.

consensus and controversy –   .Kohlberg's theory .variety of moral issued .Gilligan's theory . IPR AND BIOSAFETY Credits: 4:0:0 Unit-I Engineering ethics Senses of “Engineering Ethics”' .moral autonomy .types of inquiry .  Code BT222 BT223 BT224 BT225 BT226 BT227 BT228 BT229 BT230 BT231 BT232 BT233 BT234 BT235 BT236 BT237 BT238 BT239 BT240 BT318 BT319 BT320 BT321 BT322 BT323 BT324 BT325 BT326 BT327 BT328 BT329 BT330 BT331 BT332 MT333 MT334 ADDITIONAL SUBJECTS Subject Name Bioethics. IPR and Biosafety Plant Biotechnology Applied Plant and Animal Biotechnology Introductory Genomics and Proteomics Nano Biotechnology Introductory Microbiology Introductory Biotechnology Introductory Cell Biology Introductory Genetic Engineering Role of Biotechnology in Environment Basics of Biomolecules Gene and Gene Expression Introductory Microbial Biotechnology Applied Biotechnology Mass Transfer Operations Protein Engineering Enzyme Engineering and Technology Bioprocess Engineering Immunology and Immunotechnology Advanced Environmental Biotechnology Advanced Plant and Animal Biotechnology Anatomy and Physiology Biochemistry and Human Physiology Lab Pathology and Micro Biology Hospital Management Radiological Equipments Diagnostic and Therapeutic Equipment Computers in Medicine Pathology and Microbiology Lab Medical Physics Biomaterials & Artificial Organs Bio Reactor Design Advanced Chemical Reaction Engineering Molecular Biology & Genetic Engineering Molecular Biology & Genetic Engineering Lab Downstream Processing in Biotechnology Credits 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 3:0:0 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 3:1:0 4:0:0 0:0:2 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 0:0:2 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 0:0:2 4:0:0 BT222 BIOETHICS.moral dilemmas .

legume symbiosis. Unit-IV Global Issues Multinational corporations . Micro propagation. Unit III: Application of Plant Biotechnology Hairy Root Cultures and Secondary Metabolite production. levels of containment Text Books: 1. Identification of directions for yield effect in agriculture. Somatic Embryogenesis. Hairy Root Culture. Gemini viruses etc. Agro bacterium mediated gene transfer and cloning. Gene Banks. Agro bacterium types. Biosafety. Experimental protocol approvals. Unit-V The Legal And Socioeconomic Impacts Of Biotechnology Public education of the process of the processes of biotechnology involved in generating new forms of life for informed decision making Biosafety regulation and National and international guidelines r-DNA guidelines. Cell and Tissue Culture. Plant as Bioreactors.  Models of Professional Roles .weapons development . Bioremediation. Regulation of NIF and NOD Genes. aquaculture etc.engineers as managers-consulting engineers-engineers as expert witnesses and advisors moral leadership-sample code of Ethics (Specific to a particular Engineering Discipline). Germplasm conservation. Crop improvement.theories about right action .Environmental ethics .customs and religion . 1988. Callus Culture. Biodiversity and farmers rights. TMV. " Biotechnologies in developing countries present and future ". N2 Fixation. Culture Medias. CAMV. Plant Vectors and plant Viruses Vectors and Genetic Engineering.computer ethics . Beneficial applications and development of research focus to the need of the poor. Sasson A.   Department of Biotechnology 456 . Types of Methods in plant Biotechnology. BT223 PLANT BIOTECHNOLOGY Credits: 4:0:0 Unit I: Cell and Tissue Culture Definition and need. Unit II: Plant Genetic Engineering Tools. 1993. UNESCO Publications. BBTV. Plant viruses and Genetic Engineered viruses as a tool of deliver foreign DNA. major plant viruses. 2. Sasson A. UNESCO Publishers.edible Vaccines.Self-interest . Legal implications.uses of ethical theories Unit-II Environmental Aspects of Biotech Applications Use of genetically modified organisms and their release in environment Special procedures for r-DNA based product production. International conventions patents and methods application of patents. Unit-III Intellectual Property Rights TRIPS. “Biotechnologies and Development ".

drought etc. salinity.  Unit IV: Molecular Aspects of Disease Susceptibility and Resistance Transposable elements. Dubey and Maheswari. Blackwell Scientific Publication. Aseptic Techniques. Cambridge University Press. R. Unit – III Plant Genetic Engineering In-vitro Methods in plant tissue culture. 1985. Introduction to Microbiology. 7.K. Applications of transgenic plants. Molecular Biology and Biotechnology.L. Watermelon. Mantal S. flavors. 1989. Plant Biotechnology. 1985. S. Biotechnology – basic needs of human beings (food. regeneration and viability test. somatic hybridization.Somaclonal Variation and their significance. Principles of Plant Biotechnology..H. Dodds J. insecticides. Glick and Pasternak..development of disease resistance plants by introducing Bacillus thuringiensis genes. Role of tissue culture in agriculture. 3. Transgenic plants Technique of transformation – Agrobacterium mediated and physical Methods. Pests and insects resistance. and use of growth regulators (Auxins..2003.A. anticancer agents and pharmacologically important compounds. Panima. Grieson. Marx J. Bioinsecticdes and Biofertilizers . Mathew J. horticulture and forestry. 6. methods of protoplast fusion –chemical and electro fusion. Nutrient media. plant cell cultures for the production of useful chemicals : pigments. Embryo and Endosperm culture and their applications Organogenesis and Somatic Embryogenesis – Techniques and applications. 5. Plant Genetic Engineering. practical application of somatic hybridization and cybridization. In-Vitro production of secondary metabolites – Techniques and significance. 4. Text and reference Books: 1. R. Cytokinin and Gibberellins). Clonal Propagation of elite species (Micro Propagation). Scope and Role of Biotechnology in third world countries.CHAND.H. BT224 APPLIED PLANT AND ANIMAL BIOTECHNOLOGY Credit: 4:0:0 Unit –I Introduction Biotechnology. 8. factors influencing disease resistance and susceptibility RFLP Unit V: Transgenics Stress tolerance-Biotic and abiotic temperature. 2002. Cambridge University Press. Walker and Raplery. Revolution in Biotechnology..A.viral resistance. Herbicide resistance and stress tolerance in plants..C. Edible Vaccines from plants – Banana. An Introduction to Genetic Engineering in Plants. Protoplast Culture – Isolation.Mickee R. Molecular Biotechnology. 2. Microprojectile and electroporation)   Department of Biotechnology 457 .In-Vitro fertilization – Ovary and Ovule culture.Gupta. perfumes. Introduction to Biotechnology. shelter & medicine) Unit – II Plant Tissue Culture in Biotechnology Organ Culture – Anther..

isolation of tissue. Neurobehaviour. Dubey. Boston. Kumar H. xenotransplantation. Eye. Bhan (1998) tissue Culture. Natural media –Plasma Clot. Elsevier..C (1996) Plant Tissue Culture. copyright. Text Books 1.P. Meerut. cancer – oncogenes. International Depositories and their Interlink..V (1997) Biotechnology applications of Plant Tissue &cell culture. Unit – V Animal Genetic Engineering Techniques for gene transfer in animals. Carcinogenicity and Mutagenicity. LT50. 2. patenting genetically engineered animals. Text Book of Biotechnology.Ignacimuthu. Pvt. Chand & Co. and Sdava D. and mechanical disaggregation. New Delhi.  Unit – IV Animal Cell Culture and Gene Cloning in Biotechnology Culture Media for growth of animal cells 1.E. Cambridge 3. Cambridge University Press.E. trade secrets.   Department of Biotechnology 458 . Bojwani. 5. Phytoxicity. Grieson. 7. 4. Reproduction. 3. Oxford and IBH Publishing Co. biological fluids tissue extract. Narosa Publishing House. Rastogi Publications. and cloning disaggregation of tissue. 1990. and S. Reinert J. S. LD50. Doyle. Jones and Barlett Publishers. 1985. 4.R. Molecular Biotechnology. Toxicology . 8. b) Secondary Culture – transformed animal cells and continuous cell lines transfection of animal cell lines.D (1991) A test book on Biotechnology (2nd Edition). Pvt Ltd. 5. Gupta P. 2004. Affiliated East West Press Private Ltd. Grierson. 11. and Bajaj Y. Ravishankar G.K. Tissue. Plant Biotechnology.J. Genes and agriculture. S.D.S. enzyme disaggregation. Reference 1. Pvt. 10. Chrispeel M. Selectable Makers and Transplantation of Cultural Cells. and Organ Culture.J. R. Mittal Publications. Hay and B. GAA TRIPS. In vitro fertilization and embryo transfer.. (1994 Plants.A and Venkataraman L.N.C. Oxford & IBH Publishing Co. Dermal. Vaccines and hormone signal transduction. LC50. mapping of human Genome. Glick and Pasternak. Importance of Serum in media 2. 6. AIDS. Dodds J. Amsterdam 2. Chemical defined media a) Primary Culture – Cell lines. intellectual property Rights (IPR) and patenting Biotechnology and Intellectual property rights Patents. Islan A. convey : plant Biotechnology in Agriculture 9. Kirsop 1990 Living Resources for bio technology Cambridge University press. Oxford & IBH Publishing co. Lydiane Kyte & John Kleyn (1996) Plants from test tubes. An introduction to Micropropogation (3rd Edition) timber Press. Mucous. Ltd.Basics of Biological Patenting.H.S. Plant Genetic Engineering. Plant Biotechnology. Ltd. proto – oncogenes and tumor suppressor genes. New Delhi. Elements of Biotechnology. (2004). 2004. HAT selection. Plant Tissue Culture: Applications and Limitations. choice of Intellectual property (IPr) and plant genetic resomes (PGr) . Partland. S.S (1997) Applied and fundamental Aspects of Plant Cell. trademark. A.

PDB.. Dubay and Maheswari. Some specific metabolic databases Reference: 1. Genome Marking and mapping techniques Unit III: Mapping and sequence assembly – genetic marker– Physical Marker –. Panima. 13. 2-D PAGE databases.and Cterminal sequence tags .C. David W Mount. Molecular Biology and Biotechnology. Introduction to Biotechnology. chromosome jumping. OMIM. TrEMBL. BIOS Scientific Publishers Ltd.proteome and technology .Gupta.information and the proteome – Primary attributes for protein identification .Modifications that influence protein change on 2-D PAGE. Proteomics from Protein Sequence to Function. Viva Books Ltd. 14.K. T.A Brown . Walker and Raplery. 2. BLOCKS. specialized protein sequence databases. Human Genome Project Transcriptome– structural & functional Genomics – comparative & population genomicsPharmacogenomics – Phylogenetics. Metabolic databases. Introduction to Microbiology.cross species protein identification .2003. Radiation hybrids – Sequence markers.protein species of origin . PROSITE. R. 2nd Edition. 2nd edition. 2002. SWISS-PROT. directed short gun & Clone contig approach – Primer walking.CHAND. 2002. Nucleotide & protein sequencing methods – Chemical.Protein N. 2002 3. chromosome walking. S. enzymatic. CBS publishers. 2nd edition. Expression analysis – micro array approach.  12. Genome. R. Pennington. Bioinformatics: sequence and genome analysis. Unit V: Proteome databases: protein sequence database. Contig assembly. BT225 INTRODUCTORY GENOMICS AND PROTEOMICS Credit : 4:0:0 Unit I: Overview –Variation and regulation in Prokaryotic & Eukaryotic genome expression profile– Brief outlook of various genome projects and their outcome. Unit IV: Introduction to Proteome . 2004 BT226 NANOBIOTECHNOLOGY Credit: 4:0:0   Department of Biotechnology 459 . genomic databases. high through put method – Automated sequencing methods Unit II: Various Approaches in sequencing genome – shotgun.

Nanobiosensor and Nanofluids. 2007. Katsunori Shimohara. Toxic effects of Nanomaterials. Unit V Ethical Issues in Nanotechnology: Introduction. Nanotechnology: Volume 5: Nanomedicine and nanotechnology. Ethical Issues in Nanotechnology: With special Reference to Nanomedicine. 2008. Silicon. Applications and Perspectives. Unit III Application: Nanomedicine. CRC Press. Nanomedicine. Nanocomputers. Nanocrystals in biological detection.  Unit I Introduction. John Wiley & Sons Limited. Titania. American Scientific Publishers.Future nanomachine. 2005. Techniques to Synthesize Nanoparticles.Applications in cancer biology. Benefits and challenges in Molecular manufacturing: The Molecular assembler concept. Biomolecular Computation by Nanobiotechnology. Handbook of Nanostructural Biomaterials and Their Applications in Nanobiotechnology. Alumina. Dental care. Artificial neurons. 2. Fabrication of Novel Biomaterials through molecular self assembly. Social and Ethical Issues. Silver. Silicon based Technology. Real-time nanosensors. Nanosurgical devices. Nanobiotechnology. Other Issues.MEMS/NEMS. Controversies and confusions. History of Nanotechnology. Applications.Small scale systems for in vivo drug delivery. Importance of Nanoscale and Technology. Characterization of Nanoparticles. Nano Particles. Electronics. Soft Lithography. Wiley-VCH. Unit IV Nanobiotechnology: Clinical applications of nanodevices. Hari Singh Nalwa. Medicine. Nanomedicine Applied in Nonmedical Contexts. 4. Nano wires and Nanotubes. Nanotechnology in Different. Scope and Overview. Jian-Qin Liu. Nanobiotechnology: Concepts.2005. Gold. Unit II Nano Particles :Introduction. Power storage. PDMS Molding. Zinc oxide. Visions and Objective of Nanotechnology. Economic Impacts. Nanoscale technology in Biological Systems. Nanotechnology and Future Socio-economic Challenges References 1.   Department of Biotechnology 460 . Nanotechnology products Latest Developments in Nanotechnology. Iron oxide. Synthetic retinyl chips based on bacteriorhodopsins. Pure Metal. Edited by Viola Vogel. Significance of Nanoparticles Nanofabrications. Understanding advanced capabilities. Self assembled monolayers/ Dip. Types of Nanoparticles. High throughput DNA sequencing with nanocarbon tubules. Greco. Artech House. Length scales . Niemeyer. Socioeconomic Challenges. Silica. Cobalt. Ralph S. Edited by Christof M. 2006.pen Nanolithography. Nano-Biodevices and Systems. Metal Oxides. 3. Fields: Automobile. Social Issues Relating to Nanomedicine. Mirkin. Atomic Force Microscopy. Future of Nanotechnology: Nano Technology Revolution. 5. Electrochemical DNA sensors and Integrated Nanoliter systems. Chad A. Materials.

Biofertilizers. sporulation.Tests for sensitivity to antimicrobial agents and its quality control. Preservation of food.Antimicrobial resistance . Nanotechnology:A Gentle Introduction to the Next Big Idea by Mark Ratner and Daniel Ratner.Biogenesis-contributions of Louis Pasteur . Springer 2006 References: Singh K. viral. BCIL.Five kingdoms .History and recent developments . pili. 2002 10. 2003 7. cilia. Nanophysics and Nanotechnology.L. CRC Press 2007 12. fungal pathogens. Lazaro Spallanzani. 10. Nature Biotechnology Volume 21.species concept .Description of organisms . Antimicrobial chemotherapy Antibiotics .cell theory Binomial nomenclature of microbes . flagella. Culturing methodology and sterilization techniques. capsule. Microbial Nutrition and syntesis of important biological molecules Unit III Microscopy: simple. Unit II Anatomy of prokayotes and eukaryotes .spontaneous generation . September 2001 8.Phase contrast Fluorescence and Electron Microscopy. Nanotechnology in Biology and Medicine: Method .source . Joseph Lister .  6. Devices and Applications by Tuan Vo Dinh. New Delhi. Sulphur bacteria.Vol I &II by CNR Rao. Microbes in the production of   Department of Biotechnology 461 .Leewenhoek. compound light microscopy . The Chemistry of Nanomaterial: Synthesis. Sewage disposal and treatment-Physical and biological. Unit V Microbes in soil.mode of action . 3. Unit IV Host microbe interaction.Properties & Applications. Free Electives BT227 INTRODUCTORY MICROBIOLOGY Credits: 4:0:0 Unit I Definition and scope of Microbiology . slime layer. Prentice Hall. No. Ratner M. cytoplamic membrane and cytoplasmic inclusions.classification .structure and function of cell wall.Dark ground . Microbial kingdoms . “Intellectual Property Rights on Biotechnology ". Wiley 2006 11. Wolf. ISBN :031014005 9. Scientific American Volume 285 No. Methods of purification of air.Microbes in air.Classical approach with examples. Ratener D (2003) Nanotechnology. bacterial. E. John Tyndall. nitrifying and denitryfying bacteria.Robert Koch.A Gentle Introduction to the Next Big Idea. Nitrogen-fixing.

BT228 INRODUCTORY BIOTECHNOLOGY Credit 3:0:0 Unit – I Definition – Scope – Biotechnology in India – Applications of biotechnology: disputed parenthood. Vitamins – Cyanocobalamin. W.P. Biological nitrogen fixation – basic requirements – symbiotic and nonsymbiotic nitrogen fixation – production and application of rhizobium as a biofertilizer – production and application of ethanol as bio--fuel. Chopra. fishes and poultry. Unit – IV Integrated nutrient supply and IPM system. Enzyme – Amylase Text Book 1. insecticide and virus resistant plants – Transgenic animals mice. Casita.  organicacids – Lactic acid. PJB 8 – SV40. Balasubramania. Oxford & IBH. 1996. cattle. S. (2003) Budha Academic Enterprises. Genetic Engineering..Gene cloning vectors – Plasmids PBR 322.N.L. Talaron K. Kathmandu. Sulphur – Solubilization. antibiotics -Penicillin and alcoholic beverages – Beer and Wine.   Department of Biotechnology 462 . Pelczar MJ. and Narim. Dharmarajan. Biological Databases and Websites. Foundations in Microbiology. New Delhi.C. 2. Pelczar and Reid. Genetic Engineering and Biotechnology.Brown Publishers. India.. Unit – III Transgenic plants – herbicide. Potash. Talaron A. Tata McGraw Hill Edition. 1989. BUC 8 – cosmids PLFR 5. University Press (India) Ltd.O. D. Nepal. M. New Delhi. Hyderabad. Chan ECS And Krein NR. Reference Books: 1. Unit – V Gene Banks – importance – DNA finger printing – methodology and application –methods of gene therapy – biosensors – types and application of biochips.. “A Text Book of Biotechnology”. A.amplification – expression vectors. 3. Unit – II Principles and Methods of gene cloning – application. Concepts of Biotechnology. Viswanathan & Co. Prasad B. G. 1996. Phosphorous. U. Socio-economic issues of Biotechnology. 2. References 1. BPV – their salient features and uses – enzymes in gene cloning – transfection . Box 20195. criminology & taxonomy. 1993. Microbiology.

Concepts and Experiment”. 6.   Department of Biotechnology 463 . Tata Mc Graw Hill Publishing Co.S and Manikkili. Rastogi Publications. cyclic AMP. 7. Gupta P. 1999. Biotechnology. Structure. fungi. “Cell and Molecular Biology” 6th Edition. A Text book of Biotechnology.M. Saras Publications. Wadsworts. E. Chellammal K. Baltimore D. Organization & reproduction of bacteria. Inc. S. Wolfe. 1990 Kimball I. Reference Books Gerald Karp and Nancy L Puritt. plant viruses and animal viruses. M. New Delhi.. Stephen L. W.D. paracrine and endocrine models of action – Quantitation and characterization of receptors Unit V Signal Transduction Signal amplification pathways. Chimeeraa. Chand & Co. “Molecular and Cellular Biochemistry”. Inc. BT229 INTRODUCTORY CELL BIOLOGY Credits: 4:0:0 Unit I Cell Structure And Functions Of Organelles Eukaryotic and Prokaryotic cells – Principles of membrane organization – membrane proteins (Internal and external) – Cytoskeletal proteins – extra cellular matrix. Vijayaraman. Unit II Cell division – mitosis and meiosis – cell cycle and factors that control cell cycle. role of inosital phosphates.  4. 1993. Unit IV Receptors And Models Of Extra Cellular Signaling Cytosolic – Nuclear and membrane bound receptors Autocrine.F.K. 1998. “Cell Biology”. Trevan. Text Book of Biotechnology.D.C. Arumugam. Wesely Publishers. Elements of Biotechnology. The Biological Principles. Philadelphia. East West Affiliated Press. 8. 2004.Toxins and their mode of entry into cell. 5. California. Uyiriyae Thozhilnutpam.W.. Dubey.. Unit III Transport Across Cell Membranes Passive and active transport – permeases – sodium – potassium pump . G proteins and calcium ion role in cell signaling. De Robertis. 1989. “Molecular Cell Biology”. (Cholera and Diphtheria).D. P. Meerut. bacteriophage. 1993. 2004. John Wiley and Sons. 9. Saunders College. E. Trichy. 1998. yeast. Text Books Dornel J.P and De Robertis. Lodish H.. R. “Cell and Molecular Biology . cyclic GMP. Freeman. Biotechnology. phosphorylation of protein kinases. 1990.H. Kumar H. algae.

An Introduction to Genetic Engineering. Probe preparation-Radioactive labels-nonradioactive labels-Different labeling methods. Autoradiography. “Principles of Gene Manipulation. Southern hybridization-Northern hybridization.Brown 3. Unit II Cloning Vectors-Prokaryotes Plasmid biology-Plasmids as vectors. Applications of rDNA technology in Diagnostics. Genetic diversity. Environmental Pollution. air. Ligases-Modifying enzymes. Mammlian cells-SV40. oil and heavy metal. S. Chand & Co. Genomic DNA library.Primrose SB.W. 2. R. Gupta P. transfection-Recombinant selection and sereening. Unit V Gene Modification & Application Of Recombinant Dna Technology Mutagenesis-Deletion mutagenesis. cDNA synthesis strategies -Linkers-Adapters-Homopolymer tailing.  BT230 INTRODUCTORY GENETIC ENGINEERING Credits: 4:0:0 Unit I Basic Tools In Genetic Engineering Restriction enzymes-Classification-nomenclature.   Department of Biotechnology 464 . Gene Cloning by T.Cloning vectors in streptomycetes Unit III Cloning Stratergy Construction of recombinant DNA: Preparation of competent cell-Transformation.PBR322. Causes and Effects of Soil. Genetic Engineering by Nicholas. Types. water. Meerut. Reference Books 1. Text Book of Biotechnology.pUC vectors-Other artifical plasmids-Phage-Lifecycle-M13-Lambda phages-Invitro packaging-CosmidsPhasmids-Cloning vectors in Gram positive bacteria. Site directed mutagensis-Its applications.K.C. Old R.A. Dubey.Backwell Scientific Publications BT231 ROLE OF BIOTECHNOLOGY IN ENVIRONMENT Credits: 4:0:0 Unit I: Environmental pollution and social Issues Definition and Scope of Environmental Biotechnology. Oligonucletoide derived mutagenesis. cDNA library. 2. 2004. PCR-different kinds-Application. DNA finger printing-RFLP Analysis-chromosome walking. Rastogi Publications. Pollution. Pathogensis. control measures. Therapeutic proteins-Vaccines Text Books 1. Elements of Biotechnology. DNA sequencing.Derivatives of pBR 322. 2004. Gene transfer techniques. Unit IV Expression Vectors Eukaryotic cloning vectors-Prokaryotic expression system-Eukaryotic expression vectorsYeast expression system-Retroviral vectors-Bacullovirus.

Structure.K.   Department of Biotechnology 465 . Dubey. Species. COD BOD and BOD sensors. glycogenesis. respiratory chain. 4. pyrimidines. R. Gupta P.C. Unit IV: Biodiversity and Conservation Definition. Environmental Biotechnlogy. Methods of energy production. Text and Reference Books: 1. lagooning. 2006. di. BT232 BASICS OF BIOMOLECULES Credits: 4:0:0 Unit I: Structure and Properties of Carbohydrates and Lipids Structure and properties of mono. nucleosides. classification and properties of fatty acids.In situ and Ex situ. Conversion of Solid Waste to Methane. Biofuels. Unit II: Structure and Properties of Proteins and Nucleic Acids Structure. diagnostic and therapeutic applications of enzymes. Chand & Co. Indu shekhar Thakur. Structure and properties of purines. Energy and Solid waste Management Biomass waste as renewable source of energy. 2.  Social Issues. Ecosystem. Unit 5: Bioremediation and Biodegradation Definition. VOC. sphingolipids. Johnware D. IPR and Patenting. Unit II: Industrial Waste Water Management Purification of waste water. Genetic. Unit IV: Enzymes and Vitamins Classification. TCA cycle. glycogenolysis pentose phosphate pathway. 2004. gluconeogenesis.Land filling. Unit III: Biomass. properties. 3. Loss of Biodiversity and its causes threats to Biodiversity. Types. Environmental Biotechnology. nucleotides. Acid Rain. Text Book of Biotechnology. Biodiversity at Global Levels.Green House Gases. Biogas production. Global Warming. deoxy ribonucleic acids. Composting and Vermi Composting. Bioenergetics: redox biochemistry. Management of radioactive pollutants in water. Types. 1987. Values of Biodiversity. energy rich compounds. Meerut.A. phospholipids. oxidative phosphorylation . lipoproteins and steroids. oligo and polysaccharides.Basic concepts and Applications. Unit III: Intermediary Metabolism and Bioenergetics Carbohydrate Metabolism: Glycolysis. nuclear accidents and holocaust. Foster C. S. Urea Cycle. glycolipids.Ellis Harwood Ltd. Elements of Biotechnology. Management of Sludge and Solid waste treatment. Biodiversity and its Conservation. and properties of amino acids and proteins. Aerobic and anaerobic treatments. ribonucleic acids. Rastogi Publications. genetically Engineered Microbes for Bioremediation.Ex situ and In situ Bioremediation. Hotspots in Biodiversity.F. 2004. classification. Ozone depletion.

Lubert Stryer. Murray. Biochemistry. Hershey and Chase. Rodwell. Chand & Co. 2. biochemical action and biochemical functions of estrogen. Prentice Hall International. Principles of Biochemistry 2. different types of mutation. Uppala Author Publishers Interlinks(2003) References: 1..K...A.U. Voet and Voet. calcitrol (group I hormones).Transcription And Translation Synthesis and processing of different forms of RNA. Meselson and Stahl experiment. Biochemistry. Dubey.C. 2nd edition. Unit II Replication Of Nucleic Acids Semi conservative replication.   Department of Biotechnology 466 . 2nd Edition. different forms of DNA and RNA. Harper’s Biochemistry. Unit IV Splicing And Mutation RNA splicing-retroposons.. 3.. different models of replication in prokaryotes. P. Molecular basis of mutation. 2000. Translation in Prokaryotes and Eukaryotes. Granner. BT233 GENE AND GENE EXPRESSION Credits: 4:0:0 Unit I Structure Of Nucleic Acid Structure of DNA. Mayes. AL. Text book: 1.K. trp Text Books 1. 1995. their importance-Genetic codeTranscription-Eukaryotes & Prokaryotes. R. V.. Lehninger. post transcriptional modification. Satyanarayana. Dr. R. Avery. functions. Post translational modification-targeting and localisation of protein (endoplasmic reticulum lumen protein). 4th Edition. S. Telomeric DNA replication Unit III Expression . Biochemistry. Unit V Gene Regulation Gene regulation-operon concept-gal. WH Freeman & Co. dietary sources. B. Frankel and Singer. Identification of DNA as genetic material-Griffith. Nelson DL and Cox MM. John Wiley & Sons Inc. FSH and LH (group II hormones). 2004. cAMP. Text Book of Biotechnology. McLeod and McCarty. lac.W. UV repair mechanisms.  Vitamins: Classification. daily requirements and clinical orientation of water soluble and fat soluble vitamins Unit V:Hormones Hormones: Classification. androgen. replication of DNA in Eukaryotes.

Environmental Biotechnology. citric acid). Gupta P. aerobic and anaerobic bacteria. (2004). New Delhi. Preservation and maintenance of microbes.Composting. Unit-V: Biomass energy Energy sources – A general account. “Essential of Molecular Biology” 3. harvest. Panima publishing corporation. algae and fungi. biofertilizer. Biological filters. animal feed. (2003) Biotechnology: A text book of industrial microbiology. Packed column reactors. beverages (beer. Pelczar MJ. organic acids (acetic acid.A. Unit – IV: Biotechnology for solid waste management Solid waste treatment . viruses. “Molecular Biology of gene” 2. 2004.J. Wolf Cruger and Anneliese Cruger. Himalaya publishing house. single cell protein (SCP). food industries and Agriculture Production. Bioreactors for waste water treatment-Activated sludge process. Meerut. classification and nomenclature of microorganism. 2001. recover/uses and mode of action – enzymes. Bombay (1995). “Genetics – A molecular approach” BT234 INTRODUCTORY MICROBIAL BIOTECHNOLOGY Credits: 4:0:0 Unit – I: Introduction to Microbiology Basic of microbial existence: history of microbiology.   Department of Biotechnology 467 . Text Books: 1. S. oil recovery. biomass. antibiotics. India. wine). microbial mining.Ignacimuthu. biomedical waste treatment. Fluidized bed reactors (FBR). lactic acid. biopesticide. Plant Biotechnology. Rastogi Publications. Unit – III: Bioremediation Xenobiotics-microbial mechanism. Use of immobilized enzymes and microbial cells. Unit – II: Microbes in pharmaceutical. bacterial growth curve. Rotating biological contactors (RBC).K. Molecular Biology” by Weaver. Nutrient requirements of bacteria and different media used for bacterial culture. Chan ECS and Krein NR. biogas production. 2. T. Watson. Contact digesters. alcohol (ethanol). ore leaching. vitamins (B12).  2. Oxford and IBH Publishing Co. David Freifelder. oil spill remediation. Reference Books 1. 3. bioethanol and biodiesel.definition and modes for utilization of biomass. Structural organization and multiplication of bacteria. useful features of biofuel. mushroom cultivation.S. Vermicomposting. Tata McGraw Hill Edition. Pvt. membrane bioreactors. Jogdand SN. 4. Microbiology. amino acids. Ltd. Elements of Biotechnology.Brown.

and Organs Introduction to the basic principles of cell structure.K. Meerut. Himalaya publishing house. Organs and cells involved in immune responses-primary and secondary lymphoid organs. Text book: 1. Unit V: Human biology Nervous system. AH. W. New York   Department of Biotechnology 468 . Elements of Biotechnology. Biotechnology. Dubey RC.. 3.. MD.  References: 1.H. Biotechnology. J. Vernalisation – Photo periodism. 4. Single cell protein. gene regulation. Tissue culture. Prokaryotic gene expression. Overview of Immune system – Innate and acquired immunity. BIOS Scientific Publishers Ltd. Lamarckism Lysenrko. Patel. Gupta P. Micropropogation. GMOs.humoral and cell mediated immunity-active.Production of primary and secondary metabolites. the organization of cells into tissues and organs. 1993. Eukaryotic gene expression. Arumugam. 2nd Edition. India. New Delhi BT235 APPLIED BIOTECHNOLOGY Credits: 4:0:0 Unit I: Cells. and the principles of organ systems. Biofertilizers. 1994. genetic code. 2004. R. 2002. Transgenics. Unit II: Cell and Molecular Biology Introduction to the basic principles of biochemistry and molecular biology. heart and its function. Immunology. passive and adaptive immunity. Chand and Co. Isolation and cultivation of economically important microbes. S.media. Text book of Biotechnolog. circulatory system. New Delhi. Treven. Chand & Co. Dubey. 2. T. S. brain. parts of brain and their functions. 1995. Reference Books: 1. Microbial genetics. Rastogi Publications. 2. Unit IV: Plant Biology Biotechnology definition. DNA.C. Saras publication. Stem cells and applications. Genome. 2. Industrial microbiology. 2004. Kuby.A Brown . gene therapy. Tissue. Tata McGraw Hill Edition. Freeman and Company. The Biotechnology principles. callus formation and protoplast fusion. history and scope. Unit III: Genetics and Genetic engineering Principles of Mendelian inheritance and chromosomal basis of heredity. Text Book of Biotechnology.

Diffusion measurement and calculations. International edition. Industrial equipments . Unit-II Distillation. Design calculations of by McCabe –Thiele method and Ponchon Savarite methods. Molecular and Eddy diffusion in a gas and liquid . Azeotropic distillation.E Treybal. “ Mass Transfer Operations” McGraw Hill 2. W. Hydrogen. hydrophobic and Vander walls interactions in protein   Department of Biotechnology 469 . Interface mass transfer . 3. molecular distillation. Vapour – Liquid equilibrium diagram.Judson King “Separation Process BT237 PROTEIN ENGINEERING Credits: 4:0:0 Unit I Bonds and Energies in Protein Makeup Covalent. Unit – IV Adsorption Ion-Exchange. Ordinary diffusion in multicomponents gaseous mixtures. C. Theory of mass transfer . Coordinate. Credits: 4:0:0 Unit-I Diffusion in fluids. Theories of adsorption of gases and liquids : industrial adsorbents. overall mass transfer coefficient . Unit – V Leaching and Extraction: Solid – liquid equilibrium. Leaching equipment. absorption with chemical reaction. diffusion in solids. Types of distillation – Simple distillation .  BT236 MASS TRANSFER OPERATIONS. Text book. different contact Extractors. Types of packing and Characteristics . flash distillation. Steady state diffusion under stagnant and laminar flow condition.Batch and continuous types: Calculations of number of stages.L McCabe Smith and Harriot P “ Unit Operations of Chemical Engineering” Sixth edition McGraw Hill . Analgies between momentum and mass transfer coefficients. Various industrial distillation – Extractive distillation . Principles of Ion –Exchange. 2001. methods of distillation – Batch and continuous distillation. Concept of mass transfer coefficient. Theories of gas absorption. Concept of NTU and HTU. steam distillation. Adsorption equipments for batch and continuous operation. Ionic. Fractional distillation. Raoults law derivations from ideality . R. batch and continuous extractors. Desgn of absorption towers . Unit –III Absorption. Unit operation of Chemical Engineering – Chattophathya – Khanna publication Reference books: 1.Equlibrium in ternary systems .

E. multi substrate reactions. Application of enzymes in food. Immunotoxins.  structure. Freeman WH. pKa). Secondary structure: Alpha. helix-turn helix motifs in homeodomain. Zn fingers. Unit III Protein Architecture Primary structure.C. induced enzymes. estimation of Michelis – Menten parameters. Drug Designing Text Books Voet D. Unit II Mechanisms and Kinetics of Enzyme Action Mechanisms of enzyme action. imidazole groups) and peptide synthesis. and Voet G. USA. pharmaceutical and other industries. Second Edition”. Quaternary structure: Modular nature. Helix-turn-Helix motif in DNA binding. concept of active site and energetics of enzyme substrate complex formation. denaturation and renaturation. Chemical reactivity in relation to posttranslational modification (involving amino. 1999 References Creighton T. specificity of enzyme action . infrared. Second Edition.. beta-sheets.mechanisms and   Department of Biotechnology 470 . folding. “Biochemistry”. up and down & TIM barrel structures nucleotide binding folds. Third Edn. charge.J. Intracellular and Extracellular enzymes. X-ray) and elucidation of protein structure. kinetics of single substrate reactions. Types of enzymes-Constitutive enzyme. beta-turn-beta (hairpin). ultraviolet. Unit IV Structure-Function Relationship DNA-binding proteins: prokaryotic transcription factors. and Wilkinson A. carboxyl.E. 1993 Moody P. formation of complexes. Membrane proteins. Enzymes for analytical and diagnostic applications. visible. solubility. alpha-beta-alpha. Proteins. NY. John Wiley and Sons. beta and loop structures and methods to determine Super-secondary structure: Alpha-turn-alpha. Tertiary structure: Domains. 2001 Branden C. “Protein Engi BT238 ENZYME ENGINEERING AND TECHNOLOGY Credits: 4:0:0 Unit I Introduction and Application of Enzymes Classification of enzyme. Unit II Amino Acids and Their Characteristics Amino acids (the students should be thorough with three and single letter codes) and their molecular properties (size. and Tooze J.. Garland Publishing. understanding catalytic design by engineering trypsin. “Introduction to Protein Structured. topology diagrams. abzymes and Enzymes : Serine proteases. thiol. Leucine zippers. chymotrypsin and elastase Unit V Methods of Protein Engineering Methods of Proteins engineering. Immunoglobulins: IgG Light chain and heavy chain architecture. micro. hydroxyl. Interaction with electromagnetic radiation (radio. Eukaryotic transcription factors.

oxygen mass transfer in bioreactors . covalent binding etc. product. Scale up criteria for bioreactors based on oxygen transfer. Douglas S. Ellis Horwood Pub BT239 BIOPROCESS ENGINEERING Credits: 4:0:0 Unit I Design and Analysis of Bioreactors Design and operation of novel bioreactors-Air-lift loop reactors. Flow injection analysis for measurement of substrates. mass transfer correlations. cross-linking. Blanch. “Biochemical Engineering Fundamentals”. packed bed reactor. “Biochemical Engineering”. State and parameter estimation techniques for biochemical processes. methods of characterization of enzymes. Clark. Overview of applications of immobilized enzyme system Unit – V Enzyme Biosensors Types of Biosensors. McGraw-Hill. “Biochemical Engineering”. Fluidized bed-bioreactors. Text Books 1. RTD and stability analysis of bioreactors Unit II Bioreactor Scale-Up Regime analysis of bioreactor processes. ph and temperature effect on enzymes & deactivation kinetics. Allosteric regulation of enzymes.  kinetics. encapsulation. 2. Bubble column reactor. design of enzyme electrodes and their application as biosensors in industry. Marcel Dekker. James M. Lee. Unit III Purification and Characterization Production and purification of crude enzyme extracts from plant. animal and microbial sources. Harvey W. microbial calorimetry .   Department of Biotechnology 471 . methods for the determination of mass transfer coefficients. power consumption and impeller tip speed Unit III Monitoring of Bioprocesses On-line data analysis for measurement of important physico-chemical and biochemical parameters. USA. . types of inhibition & models –substrate.. advantages and disadvantages of different immobilization techniques. E.microbial oxygen demands. matrix entrapment. Ollis. Methods of on-line and off-line biomass estimation. 2. PHI. References 1.examples. Inc. products and other metabolites. healthcare and environment. James. Wiseman. Bailey & David F. “Enzyme Biotechnology”. turnover number. development of enzymatic assays. Unit IV Enzyme Immobilization Physical and chemical techniques for enzyme immobilization – adsorption. Monod changeux wyman model.

Modelling of recombinant bacterial cultures. 1. Bailey & David F. 2. James E. “Bioprocess Technology”. fed batch. single cell models. models of cellular energetics and metabolism. plasmid stability in recombinant cell culture. Complement and its activation. Unit II: Antigens. Springer Verlag. Harvey W. McGraw-Hill. primary and secondary lymphoid organs – Hemopoietic stem cells. Polymorphs and Platelets – Immunological memory – Differentiation of lymphocytes. Ollis.  Unit IV Modern Biotechnological Processes Recombinant cell culture processes. Marcel Decker Inc. limits to over expression. function and biological properties of Ig classes – organization and expression of immunoglobulin genes – Generation of antibody diversity – Gene segment codes for each L and H chain. Clark. Dynamic simulation of batch. Immunoglobulins: structure. “Biochemical Engineering”. opsonisation and cytolysis. Macrophages. USA. BT240 IMMUNOLOGY AND IMMUNOTECHNOLOGY Credits: 4:0:0 Unit I: Outlines of Immunology Historical perspectives and overview of Immune system – Innate and acquired immunityhumoral and cell mediated immunity-active. steady and transient culture metabolism. Douglas S. Kinetics and Reactors”. Antibodies and Complements Antigens and antigenicity :Types. Monocytes. passive and adaptive immunity – idiotypic network. “Biochemical Engineering Fundamentals”. Interactions of antigen and antibody: Primary interaction – secondary phenomenaprecipitation. Text Books 1. Bioreactor strategies for maximizing product formation. Anton Moser. “Biochemical Engineering”. James M. B cells. 3. biological role of complement components – Sequential proteolytic cascade – complement activation – classical and alternate pathways.   Department of Biotechnology 472 . References 1. Lee. guidelines for choosing host-vector systems. agglutination. structure and requirements for immunogenecity – Antibody specificity – Antigen specific receptor of antibodies – Regional variation of antigen binding site. “Handbook of Bioreactors”. Organs and cells involved in immune responses – organs of stem cell origin. T cells. Bioprocess design considerations for plant and animal cell cultures Unit V Modelling and Simulation of Bioprocesses Study of structured models for analysis of various bioprocess – compartmental models. Atkinson. plasmid replication and plasmid stability model. Blanch. PHI.

Jonathan Brostoff. Keith Roberts and James D Watson (Eds. Dennis Bray. Garland Publishing Inc.. ELISA.Saunders Company. Immunology. Unit IV. and Stewart. tuberculosis. W. macrophages and NK cells. J. Immunocytochemistry.) . Suppressor T Cells and Regulatory T cells – cell mediated cytotoxicity – Lymphokines and Cytokines – their biological role..Mosby Publication. Reference Books: (1) Ivan Roitt . Cellularand Molecular Immunology. New York. Immunology. immunoprophylaxis and immunotherapy – immunodeficiency diseases – Transplantation and rejection – Tumor antigens and immune responses to tumours – Natural and artificial immunotherapy. Unit V. Immunopathology and Immunologic disorders Hypersensitivity :Types. 3rd Edn. Antibody Technology.). New York. Cell mediated immune response : Phagocytosis – role of T cells. typhoid. Autoimmunity:Spectrum of autoimmune diseases – Immunodeficiency states – Autoantibodies in human dise– Aetiology of autoimmune responses – HLA system and disease association – MHC and its significance – class I and class II antigens of MHC. Immunology. fungi and parasites – Immunity to infectious diseases: leprosy. D. Andrew K.. David Male (Eds. bacteria. 1994. Freeman and Company.). Flurescence antibody techniques.M. Martin Raff. mechanism and disorders of hypersensitivity.1994.H. Essential Immunology.. 1993.. 8th Edn..B.1995. rabies. Immunoblotting . Churchill Livingston. Pober (Eds. (7) Kuby. Subtypes of Tcells and their functions: Cytotoxic T cells. malaria.. (3) Weir.1997. Immunologic disorders. Helper T cells.. Lichtman & Jordan S. Principles and methods of vaccine preparation – Edible vaccines. BT318 ADVANCED ENVIRONMENTAL BIOTECHNOLOGY Credits: 4:0:0 Unit I: Environmental pollution and social Issues   Department of Biotechnology 473 . 8th Edn.  Unit III: Immune Response Humoral immune response: Antibody synthesis – clonal selection – model kinetics of primary and secondary responses – Cellular Cooperation – B cell activation for T independent and dependent antigens – isotype switching. Abbas. 3rd Edn. (2) Ivan Roitt.. Julian Lewis. Immunity to infections and Immunotechnology Immunity to viruses.New York. AIDS. hepatitis. (4) Eryl Liddell and Ian Weeks. 3rd Edn. amoebiasis. 1997 . J. Immunological techniques: RIA. Molecular Biology of the Cell. (6) Bruce Alberts.1994. BIOS ScientificPublishers. W. Active immunization – Passive immunization – Polyclonal antibody production – Hybridoma techniques in monoclonal antibody production and their applications.. Blackwell ScientificPublication. (5) Abul K.

Bailey J. Vol. lagooning. McMillan Publications. Omen G. VOC. Composting and Vermi Composting. Environmental Biotechnology. Right and Unit V: Bioremediation and Biodegradation Definition. Unit III: Biomass. Aerobic and anaerobic treatments. General Microbiology.Land filling.A. Ecosystem.. Genetic.R. D. 2006. Publication & Distributors Harlow.In situ and Ex situ. Hotspots in Biodiversity. Species.   Department of Biotechnology 474 . Animal Genes and DNA Sequences. Biotechnology and Bio degradation. Biodegradation of Hydrocarbons. Causes and Effects of Soil. 2. Environmental Biotechnology. Types. Insecticides and Xenobiotics. oil and heavy metal. Wheels M. Herbicides. Ingraham J. Engg. 1986. Energy and Solid waste Management Biomass waste as renewable source of energy. 3. Chakraborty K.Y. Stanier R. genetically Engineered Microbes for Bioremediation. 2nd Ed. Ozone depletion. 1. Bioremediation of Ground Water. Environmental Pollution.F. Biofuels and Microbial fuel cells. Conversion of Solid Waste to Methane.Sharma References 1. 1987. Plant Breeders Farmers Right.  Definition and Scope of Environmental Biotechnology. Gulf Publications Co. Unit II: Industrial Waste Water Management Purification of waste water. 3. Acid Rain.D. 1999.Green House Gases. Laboratory methods for the detection of coli form organisms in water. Values of threats to Patenting. Biogas production. nuclear accidents and holocaust. Pollution. Methods of energy production. Biodiversity. 2. Indu shekhar Thakur. Water recycling methods. Types.L. Painter R. & Ollis. Biodiversity and its Conservation. Advances in Applied Biotechnology series. Biochemical Engineering Fundamentals. Management of radioactive pollutants in water. Mc Graw Hill. London 1989.Ex situ and In situ Bioremediation. Management of Sludge and Solid waste treatment. Environmental Biotechnlogy.Ecofriendly agriculture. Text and Reference Books: 1. IPR and Patenting of Higher Plants. water.S. Cellulose and Hemi cellulose as source of energy.F.E. Alam Scragg. Pesticides. Types. Organic farming BY Dr. Loss of Biodiversity and its causes Biodiversity. Biodiversity at Global Levels. COD BOD and BOD sensors.Ellis Harwood Ltd. Social Issues. 4. 1989. Longman.L. air. Johnware D. Foster C. Global Warming. Unit IV: Biodiversity and Conservation Definition. control measures.Basic concepts and Applications.

nuclear transfer for mammalian cells. 8. Plant biotechnology In Agriculture: K. New Jersey. Johnson A. Dashek W. Regulatory and safety aspects of GMOs. Properties and applications of Hairy root cultures – Bioreactors for Plant cell and Tissue cultures . Chapman and Hall. 1998. molecular biological techniques for rapid diagnosis of genetic diseases Unit V: Applications of animal biotechnology Production of biopharmaceuticals. Elsevier. Plant Tissue Culture: Applications and Limitations. Transgenics – Ethical concerns and solutions . 3. P. restriction fragment length polymorphisms. 5.Plant cell cultures for the production of food-related chemicals: pigments perfumes. 7.M.K. Singapore.J. CRC Press. Plant Biotechnology and Transgenic Plants. xenotransplantation. World Scientific Publishing Co.product safety. 1994.   Department of Biotechnology 475 . in vitro fertilization and embryo transfer. Amsterdam. Spier and J.. Animal cell Biotechnology. Methods in Plant Biochemistry and Molecular Biology. (1998) 6. 4.Protoplast isolation. culture and fusion. Grierson & S.N.S. Gene therapy – Basics and application to cancers and Tumours – Therapeutic applications of antisense nucleic acids Text Books: 1. Pvt. Jones (1990).H. plenum press Plant Molecular biology: D.V. Madras. Marcel Dekker Inc. Ti plasmid and vectors. 9. flavors Unit III: Genetic Engineering Techniques for the insertion of foreign genes into plant cells. and Holland A Animal Biotechnology and Ethics . Retrovirus vectors. and insertional mutagenesis – Transgenic plants as sources of modified oils Unit IV: Basic techniques of animal cell culture and their application Introduction of novel genes – Transfection. transposons.E. 2002. Lever. GMFs.Gupta. Introduction to Biotechnology. transgenic animals.B Griffiths. K.K. Genome Organization and Expression in Plants: C. Hui.G. Lindsey and M. Covey Blackie.Rostogi Publication 2. production of transgenic plants. Prentice hall. S. R.Importance in Bio protection and conservation Unit II: Plant cell and tissue culture Techniques involved . (i) Transgenic plants (ii) gene cloning. Ltd.  BT 319 ADVANCED PLANT AND ANIMAL BIOTECHNOLOGY Credit :3:1:0 Unit I: Introduction Plant and Animal Biotechnology . Academic Press. public perception of Biotechnology – Legislation. London. Kirsi-Marja Oksman-Caldentey and Barz W. Bhojwani (1990). Gene therapy – From Laboratory to the Clinic.. 1997.

Unit III: Digestive System: Structure of alimentary canal. Regulation of posture. Physiology of defecations. Neural control of cardio vascular system. 3. Unit II: Nervous and Sensory Systems: Structure and function of nervous tissues. structure of lungs. 1979. Reflex action. Afferent nervous systems. General circulation. Thyroid and Parathyroid glands.V. 6. neurosis. Charles A. Syril A Kalee and Eric Neil. Hongkong. 5. Warrik C.Doyle. “Applied Physiology”.Mosby Company.K. Oxford University press. Bladder and Colon. Ovary and Testis. Animal cells . Cambridge University Press.   Department of Biotechnology 476 . BT321 BIO CHEMISTRY AND HUMAN PHYSIOLOGY LAB Credit: 0:0:2 1. Regulation of breathing. Cambridge. Physiology of urine formation. The C. Samsons Wright. Physiology of emotion. “Textbook of Anatomy and Physiology in Radiological Technology”. Circuitatory and Respiratory Systems: Structure of heart. Physiology of Perspiration. 1968. Venous return. Ostelogy and Histopathology. Tongue. 4. 2. Unit IV: Excretory Systems: Structure of Kidney. sensory end organs. Testing for Detection of Glucose. multi vacuolar nature. R. Mechanism of sight. Carrier of oxygen and carbon dioxide. Regulation of temperature. multinuclear nature. Physiology of miaturation.E. Liver. Blood. hearing and smelling. Related digestive glands. Study of rate of Conduction of Nerve Impulses. Testing of various parameters of Vision and Errors of Refraction. Unit V: Endocrine System: Pituitary gland. Capillary circulation. Sam Louis. Traches and its branchings. Mechanism of alimentary canal. Dyspnoea . Henglong. Fructose and Starch. Testing of Hearing using Tuning Fork. 1977. Isolated Frog Heart Perfusion and Effect of ionic Changes. Secretion of digestive fluids. Recording of Muscle Response to Induced Electrical Stimulation 2. pancreas. Cerebro spinal fluid . Function of liver. Living resources for Biotechnology. “Anatomy and Physiology for Radiographers”.  10. References: 1. BT320 ANATOMY AND PHYSIOLOGY Credit :4:0:0 Unit I: Basics: Basic Embryology. 3. a. Kirsop (1990).Jacob. Oxford University Press.Hay and B.

health education. Unit V Microscopy Light Microscopy – Dark field Microscopy – Phase contrast microscopy – electron microscopy. Gram Stain. protozolal. – Helminthic disease.L & Ramzi S. Fluorescent techniques. health organization in state.B. Robbins S. Testing of Urine for presence of Sugar. Infection and Immunity Autosomal and Sex linked disorders – Storage disorders – Types of hypersensitivity reactions – Immune deficiency Syndrome – Primary – HIV – Viral disease.Inflammations. W. Unit III Genetic Disorders. educational responsibilities. health insurance. Difference between benign and malignant tumors – Etiology of tumors – Spread of Tumors. Saunders Co. Orient Laongman’1998. Observation of culture. staff structure in hospital Unit II National Health Policies Need for evolving health policy. health legislation Unit III Training and Management Of Technical Staff In Hospital   Department of Biotechnology 477 . “Pathologic Basis of Diseases’.R & Jayaram Panicker C. Classification.l Shock. Anatha Narayanan. Protein Estimation using Spectrophotometer BT322 PATHOLOGY AND MICRO BIOLOGY Credit: 4:0:0 Unit I Normal Cell Structure Cell Degeneration and regeneration . Unit II Fluid Aod Heamodynamic Derangement Edema. ‘Text Book of Microbiology. Text book: 1. Unit IV General Structural Organization of Bacterial. AFB Stain. General Test for Proteins 8. apoptosis. role to be performed by them in hospital. Identification of disease producing organism. Antigen – Antibody Technique. Neoplasia. Hemorrhage – Thrombus – Embolism – Disseminated intra vascular Coagulation – Hematological disorders. Viral Cell Growth and Identification of Bacteria.C. Simple Stain. BT323 HOSPITAL MANAGEMENT Credit: 4:0:0 Unit I Need and Scopes Of Clinical Engineering Clinical engineering program. health financing system. Chlamydial – Bacterial – mycoplasma – Rickettsial disease – Fungal.  7. 1999 2.R. Bleeding Disorders – Leukemia – lymphoma.

. Beta and Gamma emission. Multi section radiography. Prentice Hall of India. nuclear angiogram. steps of training. 1996 (Unit II – V). Webster J. Unit V Radiation Safety Hazardous effect of Radiation. 1997.C. Steve Webb. levels of training. laboratory administration.Hendee. Cooling system. 2. CAT. William R. and Albert M. Image Intensifier. selection of anodes. Adam Hilger Philadelphia 1988. Goyal R. Unit III Special Radiological Equipments Principle. ICRP and other standard organization. Louis. 1979 (Unit I). Multi section Radiography. St. medical records. Radiation Monitoring. dot scanners. “Clinical Engineering Principle and Practice”.S. “Handbook of Biomedical Instrumentation”.Cook. Joint Commission of Accreditation of hospitals. Heel Pattern. Principles of Radiation therapy. Third Edition.C. Plane of Movement. developing training program. X-Ray department. Unit II Radio Diagnosis: Radiography. FDA. patient data.   Department of Biotechnology 478 .. MRI Unit IV Application Of Radioisotopes Alpha. References: 1. Englewood Cliffs. Porter Bucky system. employee appraisal method. Angiography. E. Unit V Computer in Medicine Computer application in ICU. evaluation of training.  Difference between hospital and industrial organization.Khandpur. “The Physics of Medical Imaging “. 1992. R. “Handbook of hospital personal management”. methods to monitor the standards. New Delhi. New Jersey. Unit IV Standards and Codes In Health Care Necessity for standardization. BT324 RADIOLOGICAL EQUIPMENTS Credit: 4:0:0 Unit I X-Rays Principles and production of soft and hard X-rays.. wages and salary. Scattered radiation. Text Books 1. communication.” Medical Imaging Physics”. Principle of NMR. Principle of radiation detectors. Prentice Hall Inc.Russel Ritenour. Tata McGraw-Hill Publishing Company Ltd. Fluoroscopy. simulation. Mosby Year Book. Safety Limits. Radiation protection Techniques. 3. 2.

lead system. fibrillation. RS232-C and IEEE bus standards. Micro and Macro shock.  BT325 DIAGNOSTIC AND THERAPEUTIC EQUIPMENT Credit: 4:0:0 Unit I Cardiac System ECG. pnemograph. different types of pacemakers. Brown. normal and abnormal waveform. fingerpump. roller pump. Webster. display techniques A. AC defibrillator. “Handbook of Biomedical Instrumentation”. John wiley and sons. Motherboard and its logic. Instruction set & programming. Tata McGraw-Hill. “Medical Instrumentation Application and Design”. Unit V Respiratory Measurement and Ventilator Spirometer. functioning. Unit III Ultrasonic Techniques For Diagnosis And Safety Measures Basic principles of Echo technique. References: 1. cardiac pacemaker-external pacemaker. Unit II Neurological System EEG. Unit IV Heart-Lung Machine Need for the unit. modems and networking. EMG and EEG data acquisition. Microcontrollers. Ultrasonic applied as diagnostic tool in ophthalmology. “Introduction to Biomedical equipment technology”.   Department of Biotechnology 479 . implantable pacemaker. artificial respirator – IPR type. Khandpur R. diagnosis interpretation. 1997. HDC and Post sequence. Respiratory volume measurement. New York. earthing schemes.S. 1997. system connections. functioning of bubble. synchronised and unsynchronised types. electrodes. CRT controllers. frequency bands. DC defibrillator. BT326 COMPUTERS IN MEDICINE Credit: 4:0:0 Unit I Overview of Computer Hardware Pc-At 8086 architecture. sources of ECG. storage and retrieval. 2. Patient Safety: Sources of leakage current. Echo cardiograms. FDC. epileptic discharges. 3. electronic monitoring of functional parameter. wave characteristics. disc type and membrane type oxygenerators. transmission of signal and images. M modes. diagnostic interpretation. Echo encephalogram.carr and John M. John G. genesis. John Wiley and sons. B. obstetrics and gynaecology. PC based video card. NewDelhi. defibrillator. Unit II System Design Multichannel computerised ECG. New York. monitoring circuits. 1998. spontaneous and evoked response. Joseph J.

artificially produced isotopes and its application. R. factors affecting frequency of radiation induced mutation. Tata McGraw-Hill. automated ICU. depression of Macro molecular synthesis. ANN concepts. biological effect of microwave and RF wave. “Computers in Medicine”. Principles of Nuclear Physics – Natural radioactivity. physiological system modelling and simulation. cellular effects of radiation. BT327 PATHOLOGY AND MICROBIOLOGY LAB Credit: 0:0:2 12 experiments will be notified by HOD from time to time BT328 MEDICAL PHYSICS Credit: 4:0:0 Unit I Atomic Physics . New Delhi. Unit IV Computers in Medical Systems Modeling Radiotherapy. Singapore.  Unit III Computers in Patient Monitoring Physiological monitoring. emission of light and its frequencies. Unit V Computers in Medical Research Role of expert systems. 1999. Chromosomal damage.D. 6.Hall. “Microprocessors and Interfacing : Programming and hardware”. 1999. relationship. Unit II Interaction with Living Cells Target theory. periodic system of elements. Text Books 5. computerised arrhythmia monitoring. drug delivery system. Unit III Somatic Effect of Radiation Radio sensitivity protocol of different tissues in human. McGraw Hill.   Department of Biotechnology 480 . Electromagnetic spectra. DNA damage. Traditional definition of atom. interfacing to HIS. mechanical properties of atom.Lele. Radionuclides used in Medicine and technology. pattern recognition techniques in medical image classification. blood forming organs. Douglas V. information flow in a clinical lab. single hit and multi target theory. type of radiation and their applications. lenses of eye. Gene controlled hereditary diseases. Unit IV Genetic Effect of Radiation Threshold of linear dose effect. computerised concepts. embryo and Endocrinal glands. accelerator principles. Decay series. LD 50/30 effect of radiation on skin. drug design.

hard tissue replacement implants. blood interfacing implants. adhesive. Strondsburg 1980. ceramic implant materials. exposure level. polyolefin. polyamicles .LASER PHYSICS – Characteristics of Laser radiation. internal fracture fixation devices. polymers. body response to implants. Ti-based alloys. sutures. Prosthetic Cardiac Valves. ‘Non ionising Radiation’ Adam Hilgar Brustol 1988 2. medical applications. Limb prosthesis. Plenum Press. co-based alloys.. 2. biological effects. Phototherapy. 1984. BT330 BIO REACTOR DESIGN Credit: 4:0:0   Department of Biotechnology 481 .B. joint replacements. Branski.O. woundhealing process. surgical tapes. 1980. 3. Protection standards. “Biomaterials Science and Engineering”. rubbers.P ‘Biological effects of Microwave’ Hutchinson & ROSS Inc. Moselly. medical applications. Dental Implants References: PARK J. maxillofacial augmentation. hazards and maximum permissible exposures. Unit V Artificial Organs Artificial Heart. 3 year Book Publisher Inc Chicago. Laser speckle. visco elasticity. Unit II Implant Materials Metallic implant materials. blood compatibility. Externally Powered limb Prosthesis.1. References: 1.S and Cherski. Penetration and propagation of signals effects in various vital organs.Medical Physics Vol. Unit V Photo Medicine Synthesis of Vitamin D in early and late cutaneous effects. Acryrilic. laser safety management. BT329 BIOMATERIALS & ARTIFICIAL ORGANS Credit: 4:0:0 Unit I Structure of Bio-Materials and Bio-Compatibility Definition and classification of bio-materials.  Variation in dielectric constant and specific conductivity of tissues. Unit II I Polymeric Implant Materials Polymerisation. mechanical properties. Photo hemotherapy. stainless steels. high strength thermoplastics. percutaneous and skin implants. Unit IV Tissue Replacement Implants Soft-tissue replacements. aluminum oxides. Glasser. hydroxyapatite glass ceramics carbons.

Viva Books Private Limited. hydrodynamics. ISIM (interactive simulation language). Animal cells: Animal cell bioreactors. detection and prevention of foam. 2nd edition.. oxygen transfer in a three-phase flow. time-proportional control. bioreactor sensor characteristics. non-isobaric model. oxygen transfer: isobaric method. vent gas analysis. principles of pH/redox measurement and control. mixing. analogue and digital control. fermenter content. feeding. temperature measurement and control. plant cell bioreactor requirements. Heat transfer. formulation and solution of problems by simulations. Text Books Scragg A. Edited by. “Bioreactors in Biotechnology”. agitation. plant cells: plant cell bioreactors. fault diagnosis. airflow. Unit II Bioreactor Instrumentation and Control Introduction. characteristics of plant cell suspensions. Unit IV Modeling and Simulation of Fermentation Processes Modeling. ion specific electrodes. pressure. biological control-feedback and feed forward. airlift bioreactors. plant cell bioreactor design. “Process Biotechnology Fundamentals”. digital simulation programming languages. Ellis Horwood Limited. handling noisy measurements. Unit V Plant and Animal Cell Bioreactors Introduction. animal cell bioreactor operation. principles of dissolved oxygen measurement and control. pH control. numerical solution.. England 1991 Reference Books Mukhopadhyay S. dissolved oxygen. airlift bioreactors. design and construction of the airlift-loop reactor.Introduction of airlift bioreactors. Physical control of fermentation: temperature. plant cell bioreactor operation. control algorithm-PID control. Scale up: stirred-tank bioreactors. types of bioreactors: stirred-tank bioreactors.  Unit I Introduction Introduction. determination of biomass.H. adaptive control and adaptive models. BT331 ADVANCED CHEMICAL REACTION ENGINEERING Credit: 4:0:0 Unit I Basics of Reactor Design   Department of Biotechnology 482 . three-phase flow. biosensors Unit III Methods and Strategies for Fermentation Control Introduction to control: control loop.N. Control strategy for fermentation: estimation of biomass. animal cell bioreactor design. digital simulation. alternative cultures for plant cells. Chennai 2004.

Unit V Solid Catalysts Principles of preparation. Frankel and Singer. New Delhi. replication of DNA in Eukaryotes.3rd Edn.McGraw Hill International Editions.Mions.Saville. Rate law. application to non – ideal reactors. John Wiley & Sons. 1999.1981. Translation and Gene Regulation Synthesis and processing of different forms of RNA. different models of replication in prokaryotes. Ronald. space-time and mean-resistance time Unit III Multiple Reactions Qualitative and Quantitative treatment of Series. Meselson and Stahl experiment Semi conservative replication. Singapore. Scott Fogler H.M.W. RNA splicing-retroposons. tubular and stirred tank reactors. “ Elements of Chemical Reaction Engineering”. 2.3rd Edn. Octave Levenspiel.. Concentration and temperature dependent term of a rate equation. “Chemical Engineering Kinetics”. Telomeric DNA replication Unit II Transcription. Molecular basis   Department of Biotechnology 483 . BT332 MOLECULAR BIOLOGY & GENETIC ENGINEERING Credit: 4:0:0 Unit I Structure of Nucleic Acid & Replication Structure of DNA. Hershey and Chase. Smith J. interpretation of rate data in variable and constant volume systems. different forms of DNA and RNA. “ Introduction to Chemical Reaction Operation and Kinetics”...Bradley.  Rate of a order and molecularity of a reaction. 1999. Singapore.Missen.A. 2..2nd Edn. their importance-Genetic codeTranscription-Eukaryotes & Prokaryotes.. Unit II Ideal Reactors Design of batch. McLeod and McCarty. Post translational modification-targeting and localisation of protein (endoplasmic reticulum lumen protein.A. Identification of DNA as genetic material-Griffith.Prentice Hall of India. post transcriptional modification. catalyst promoters. Parallel and Series-Parallel reactions. catalyst poison. Reference Books 1. Text Books 1. properties estimation. Translation in Prokaryotes and Eukaryotes.1995. important industrial catalyst reactions. elementary and non elementary of a reaction. “Chemical Reaction Engineering”. John Wiley and Sons.Charles. Dispersion and tanks–in-series models. Instantaneous and overall fractional yields Unit IV Non-Ideal Flow Residence time distribution: Residence time functions and relations among them. New Delhi. Avery.

Cloning vectors in streptomycetes. different types of mutation. lac.PBR322. “Molecular Biology” by Weaver. 4. Autoradiography.  of mutation. Gene transfer techniques. Pathogensis. T. transfection-Recombinant selection and sereening. Mammlian cells-SV40. Watson.Brown. DNA sequencing. Old R. DNA finger printing-RFLP Analysis-chromosome walking. PCR-different kinds-Application. Gene regulation-operon concept-gal. From Genes to Clones by Winnacker BT333 MOLECULAR BIOLOGY AND GENETIC ENGINEERING LAB Credit : 0:0:2 12 experiments will be notified by HOD from time to time   Department of Biotechnology 484 .Backwell Scientific Publications Reference Books 1. Therapeutic proteins-Vaccines Text Books 1. David Freifelder. cDNA libraryUnit V Expression Vectors & Gene Modification & Application of Recombinant Dna Technology Eukaryotic cloning vectors-Prokaryotic expression system-Eukaryotic expression vectorsYeast expression system-Retroviral vectors-Bacullovirus. Genetic diversity. Genomic DNA library.A. UV repair mechanisms.Primrose SB. Ligases-Modifying enzymes.Derivatives of pBR 322.pUC vectors-Other artifical plasmids-Phage-Lifecycle-M13-Lambda phages-Invitro packaging-CosmidsPhasmids-Cloning vectors in Gram positive bacteria. Oligonucletoide derived mutagenesis. Southern hybridization-Northern hybridization. 2. Probe preparation-Radioactive labels-nonradioactive labels-Different labeling methods.Brown 3.W. An Introduction to Genetic Engineering. Applications of rDNA technology in Diagnistics. Mutagenesis-Deletion mutagenesis. “Principles of Gene Manipulation. Site directed mutagensis-Its applications. “Essential of Molecular Biology” 3. Unit IV Cloning Strategy & Vectors of Prokaryotes Plasmid biology-Plasmids as vectors.A. Gene Cloning by T. Construction of recombinant DNA: Preparation of competent cell-Transformation. cDNA synthesis strategies -Linkers-Adapters-Homopolymer tailing. “Genetics – A molecular approach” 2. “Molecular Biology of gene” 2. trp Unit III Basic Tools in Genetic Engineering Restriction enzymes-Classification-nomenclature.

“Product Recovery in Bioprocess Technology”. process design criteria for various classes of bioproducts (high volume. centrifugation and filtration methods Unit III Enrichment Operations Membrane based separations micro and ultra filtration theory. Problems and requirements of bioproduct purification. Wankat P. Economics of downstream processing in Biotechnology. Asenjo J.C. electrophoretic separations (all electrophoresis techniques including capillary electrophoresis) hybrid separation technologies (membrane chromatography. and polymers. Wiley 1985   Department of Biotechnology 485 .M. low value products and low volume. aqueous two-phase extraction. 1993. “ Bioseparations ”. applications. dialysis. 1990. precipitation methods (with salts. organic solvents. “ Rate controlled separations ”. “ Separation processes in Biotechnology ” Marcel Dekker Inc. supercritical extraction) insitu product removal. electro chromatography etc) Unit V Product Polishing Gel Permeation Chromatography.  BT334 DOWN STREAM PROCESSING IN BIOTECHNOLOGY Credit: 4:0:0 Unit I Role of Downstream Processing in Biotechnology Role and importance of downstream processing in biotechnological processes. Crystallisation Text Books 1. physico-chemical basis of bioseparation processes Unit II Primary Separation and Recovery Processes Cell disruption methods for intracellular products. integrated bioprocesing Unit IV Product Resolution/Fractionation Adsorptive chromatographic separation processes. flocculation and sedimentation. Reference Books 1. BIOTOL Series. biomass (and particulate debris) separation techniques. characteristics of biological mixtures. 1990 2. design and configuration of membrane separation equipment. removal of insolubles.. cost-cutting strategies. high value products). 2. Belter PA and Cussler E. extractive separations. Elsevier. VCH.

  SCHOOL OF BIOTECHNOLOGY   .

metabolism-waterimportance. urea cycle. Net protein utilisation and their determinations . peptides and proteins.classification. Inborn errors of Carbohydrate metabolism . Unit III Basic concept of nutrition – Importance of nutrition and dietetics. valine. and cholesterol Biosyntheses and degradation of essential amino acids namely arginine. fortification Carbohydrates-classification.phenylalanine.bioenergetics. gluconeogenesis. metabolism. source. UnitV Nutrition for specialized purposes   School of Biotechnology 456 . energy value of carbohydrates. source. Nutrient supplementation. functions. Importance of nutrition 3..lysine.leucine. Malnutrition and its problems.methonine.Vitamins-classification. metabolism -balanced diet .TCA cycle.Lactose intolerance . source. essential amino acids.histidine. antinutritional factors.metabolism.Inborn errors of and fat metabolisms – MineralsClassification. Acceptable dietary intake – Proteins-classification. energy rich compounds. dietary fibre.isoleucine.threonine and tryptophan.  ADDITIONAL SUBJECTS   Code BC213  Subject Name Food Biochemistry and Nutrition  Credits 3:0:0  BC214 BC215 Analytical Biochemistry Lab Engineering Biochemistry 0:0:2 3:0:0 BC213 FOOD BIOCHEMISTRY AND NUTRITION Credit : 3:0:0 To enable the students to understand 1. determination of energy value. gluconeogenesis.oxidative phosphorylation.Glycolysis Embden Meyerhof Pathway (EMP).glycogenolysis. pyrimidines and nucleic acids.Recommended dietary intake . Biosynthesis and degradation of purines. Nutrients in their food and their importance. Nutritional labeling and its importance. Unit IV Fats. source-. Protein efficiency ratio. Basics of biochemistry and nutrition 2. Unit II Biosyntheses and degradation of fatty acids. Unit I Electron Transport Chain. Pentose phosphate shunt. water balance. metabolism of fat .

L. oxidative phosphorylation and triose phosphate cycle. Wahlquist M L. Alzheimer’s disease Text / Reference Books: 1. mRNA and tRNA. Functional foods and Nutraceuticals in Cancer Prevention. Principles of Human Nutrition – Second Edition. 3. purines and pyrimidines. glycogenesis and glycogenolysis. Biosynthesis and biodegradation of important amino acids.Leu. Stryer L. Unit – IV : Metabolism of Lipids. pyrimidines. Edited by Ronald Ross Watson BC214 ANALYTICAL BIOCHEMISTRY LAB Credit : 0:0:2 12 experiments will be notified by HOD from time to time BC215 ENGINEERING BIOCHEMISTRY Credit : 3:0:0 Unit . bisubstrate reactions. 1993.L. polynucleotides . Phe. Lehninger A. Second Edition. sphingolipids. glycolipids and steroids. Unit – II : Structure and Properties of Proteins and Nucleic Acids Structure and properties of amino acids. 1997 5. Trp. respiratory chain. Cox. Blackwell Publishing 6. Edited by Martin Eastwood. 1994. CBS Publications. 4. effect of pH. gluconeogenesis. M. Michaelis menton equation. peptides. phospholipids. 2. Inborn errors of their metabolism. Voet D. Proteins and Nucleic Acids Lipid Metabolism: Biosynthesis and biodegradation of fatty acids. inhibition. Principles of Biochemistry. diabetes. Rate of enzymatic reactionschemical kinetics. substrate specificity. Structure and properties of purines. Voet G.  Paediatric nutrition – geriatric nutrition – Sports nutrition – Nutrition during pregnancy Functional foods-Ageing –Theories of ageing – Nutrition and ageing – Cancer and its prevention . Tyr.M.Biodegradation of proteins and nucleic acids. Fouth edition. pentose phosphate pathway. oligo and polysaccharides. 1994.I : Structure and Properties of Carbohydrates and Lipids Structure and properties of mono.. proteins. Coenzymes. Biochemistry. Biochemistry.Urea Cycle. deoxy ribonucleic acids Unit – III : Intermediary Metabolism and Bioenergetics Bioenergetics: redox biochemistry. Nelson D. Food and Nutrition. and Cys.Age-related metabolic disorders – Nutrition in the treatment of age-related disorders like hypertension. Carbohydrate Metabolism: Glycolysis.rRNA. energy rich compounds. John Wiley and Sons. Structure and of fatty acids. TCA cycle.   School of Biotechnology 457 . di. Unit-V : Mechanism of enzyme action Enzyme – Introduction.

. Mayes. Granner.A. D. Voet and Voet. 2000.K. V.. Prentice Hall International. 1995. (2000). Murray. 3.   School of Biotechnology 458 .. A.. B. R.  Text book: Lehninger. M.K. 2nd Edition. John Wiley & Sons Inc. 2.W. Biochemistry.. New York.. Nelson. L. References: 1. and Cox. Rodwell. Principles of Biochemistry Third Edition (Freeman Publishers). M. 4th Edition. WH Freeman & Co. Lubert Stryer. L. Biochemistry. (2000). Harper’s Biochemistry. P..

plate counters. Estimating number of microorganisms. ATP determination – Rapid methods for the detection of specific organisms and toxins – Immunological methods and DNA/RNA methodology Microbiological quality control and HACCP Unit III Heat processing – Pasteurisation and appertization – determination of D and z values – heat sensitivity of micro-organisms – spoilage of canned foods – aseptic packaging Irradiation – Brief account of microwave. Carbon and Nitrogen Sources. importance of molds. water. Moisture and water activity. total cell counts and viable cell counts. Useful microorganisms – Lactic Acid Bacteria. Alternative methods – Dye-reduction tests. Gram negative facultative anaerobic Rods.: Viruses. Deuteromycetes etc. Vegetables and Vegetable products Unit V Food borne pathogens   School of Biotechnology 459 . sample size. UV and ionizing radiation High pressure processing – Pascalisation Low-temperature storage – Chilling and freezing Use of chemical preservatives. antimicrobial barriers and constituents Unit II Sources of microorganisms – Soil. Definitions Fungi as Food. mold and fungi. Plants and of animal origin. Yeast.Endospore formers Irregular non-sporing gram positive rods their role in food spoilage Molds. Fruits and Fruit products. Pulses. Natural food preservatives. temperature.  ADDITIONAL SUBJECTS   Code MB209 MB210 MB211 MB212 MB213 MB214 MB215 MB306 Subject Name Food Microbiology Fundamentals of Biological Systems Community Health and Social Awareness Cell Biology and Microbiology Lab Molecular Biology and Genetic Engineering Lab Cell Biology and Microbiology Lab Molecular Biology and Genetic Engineering Lab Molecular Biology And Genetic Engineering Lab Credits 3:0:0 3:0:0 3:0:0 0:0:1 0:0:1 0:0:1 0:0:2 0:0:2 MB209 FOOD MICROBIOLOGY Credit : 3:0:0 Unit I Micro organisms associated with foods: Bacteria – Gram negative aerobic Rods. Intestinal viruses. electrical methods. Redox potential. sampling.Factors affecting growth of bacteria. Bacterial viruses and Fungal Viruses . Nuts and Oilseeds. Definitions. pH conditions.Gram Negative anaerobic rods . aseptic collection of samples. Brief overview of Modified storage packaging Unit IV Microbiology of Food commodities Cereals. mold and Yeast – nutrition. Molds in the category of Zygomycetes.

signaling by hormones and neurotransmitters. Unit –V Molecular Biology Replication in prokaryotes and eukaryotes Transcription. Cell cycle and regulation. elongation.Frazier: Food Microbiology (II edition or later) Mcgraw Hill Book Company. Agarwal.gram staining. Adams and M. Moss. Panima Publishing corporation. acid fast and capsular staining Physical and chemical control of microorganisms. S. Verma. P. Genetics and molecular Biology. termination. nucleus). Camphylobacter. Brucella. New York (1968) 2. Bacillus cereus. cell division.S. Clostridium botulinum. Microbial biosensors Unit – IV Molecular Genetics Mendelian inheritance. M.R. Chand and company 2000. Gustavo F Gutierrez-Lopez. Salmonella. Cell Biology. Cellular membrane – Structure and functions. Gustavo V Barbosa-Canovas Food Science and Food Biotechnology: CRC Press 2003 2. Third reprint 2004 References: 1.O. G protein receptors. Bibek Ray: Fundamental Food Microbiology. C & MP and inositol phosphates as second messengers.C. Scombrotic fish poisoning. V. (Third Edition) CRC Press December 2003 MB210 FUNDAMENTALS OF BIOLOGICAL SYSTEMS Credit : 3:0:0 Unit – I Biology of cells Structure of prokaryotic and eukaryotic cells overview of organelles (Mitochondria.K.  Aeromonas hydrophila. Gastroenteritis viruses. chain elongation of gene expression – Lac operon and Try operon Text Book . Hepatitis A and B. role of membrane proteins as receptors. New Delhi. protein kinesis. Spongiform encephaolpathies Text Books 1. Escherichia coli. principle of different staining techniques. Second Edition.initiation. DNA as genetic material. Golgi. Chloroplasts. ER. crossing over prokaryotic and eukaryotic genome organization. endocytosis.transport. light and electron microscopy.mitosis and meiosis Unit –II CELL – CELL interactions and signal transductions Intercellular junctions. Staphylococcus aureus. Food Microbiology.   School of Biotechnology 460 . Unit – III Microbiology Classification and nomenclature of micro organisms. W. Difference between plant and animal cell. Translation – Initiation.

MMR. Etiology. age-specific mortality rates. Ford Foundation. ARI.  Reference Books 1. Rockefeller Foundation. Poliomyelitis. FAO. fertility rates. Zipursky SL. Molecular cell Biology. Pelzer. Vidhyapooshan Sachdava. MJ. Prevention and Control of Communicable Diseases. death rates. New Delhi 2000. MB211 COMMUNITY HEALTH AND SOCIAL AWARENESS Credit : 3:0:0 Unit – I Epidemiology. Reference Books: 1. etc. Matsudaria Kaiser CA. Unit – II Health planning in India including various committees and National Health Policy and Health Goals set from time to time. Tata Mc Graw Hill Publishers. Rabies. An introduction to Sociology MB212 CELL BIOLOGY AND MICROBIOLOGY LAB Credit: 0:0:1 6 experiments will be notified by the HOD from time to time MB213 MOLECULAR BIOLOGY AND GENETIC ENGINEERING LAB   School of Biotechnology 461 . Diarrhoea.Organized sector with reference to centre. AIDS. Tuberculosis. Unit-IV Environmental sanitation. WH Freeman and company. Viral Hepatitis. Nutrition. like Malaria. Prevention and Social medicine. UNFPA. Reproductive and Child health Unit – V Objectives and organization of important agencies. 2. ILO. Indian Red cross Society. 2004. Microbiology. fifth edition.. Measles. Kriegar M. Pathogenesis. etc. Chan ECS and Krein NR. CPR. and their role in Health care activities in India. UNICEF. Family Welfare and Planning. Scott MP. Lodish H. Darnell J. CARE. Dengue.Organization and functions of community health centers and primary health centers Unit – III Problems of Population growth. 2. New York. Park&Park. Bert A. World Bank. Asia Development Bank. Cholera. District and Block level structures and local bodies and Panchayat Raj. Leprosy. etc. State. like WHO. Birthrates.

  Credit: 0:0:1 6 experiments will be notified by the HOD from time to time MB214 CELL BIOLOGY LAB AND MICROBIOLOGY LAB Credit : 0:0:2 12 experiments will be notified by the HoD from time to time. Optimization of time of induction for ß-galactosidase expression MB306 MOLECULAR BIOLOGY AND GENETIC ENGINEERING LAB Credit : 0:0:2 1.Coli) Isolation of total RNA from eukaryotic tissue Quantitative and qualitative analysis of isolated genomic DNA using spectrophotometer and by agarose gel electrophoresis 5. Extraction of proteins from plant or animal tissue and confirmation with qualitative tests 7. 4. 2. Screening of recombinant transformants by Blue-White selection method 15. Transformation competent cell preparation 13. Ligation of fragmented pUC19 plasmid DNA and testing with agarose gel electrophoresis 12. Elution technique for separated DNA from agarose gel 6. 2. Transformation of recombinant plasmid DNA into competent cells 14.Coli 10.Coli) Isolation of total RNA from eukaryotic tissue Quantitative and qualitative analysis of isolated genomic DNA using spectrophotometer and by agarose gel electrophoresis 5. Isolation of genomic DNA from eukaryotic tissue (Plant or Animal) Isolation of genomic DNA from microorganism (E. Restriction enzyme digestion of pUC19 plasmid DNA and testing with agarose gel electrophoresis 11. 3. 4. Western blotting for tissue extracted proteins or serum proteins separated in SDS-PAGE 9. 3. Separation and identification of serum proteins by SDS-PAGE using Coomassie Brilliant Blue stain 8. Polymerase Chain Reaction (PCR) for the given standard DNA using PCR kit and verifying with agarose gel electrophoresis 16. Optimization of inducer concentration (IPTG) for ß-galactosidase expression 17. MB215 MOLECULAR BIOLOGY LAB AND GENETIC ENGINEERING LAB Credit : 0:0:2 1. Isolation of genomic DNA from eukaryotic tissue (Plant or Animal) Isolation of genomic DNA from microorganism (E. Elution technique for separated DNA from agarose gel   School of Biotechnology 462 . Isolation of plasmid DNA from E.

Separation and identification of serum proteins by SDS-PAGE using Coomassie Brilliant Blue stain 8. Ligation of fragmented pUC19 plasmid DNA and testing with agarose gel electrophoresis 12. Restriction enzyme digestion of pUC19 plasmid DNA and testing with agarose gel electrophoresis 11. Transformation competent cell preparation 13. Isolation of plasmid DNA from E. Optimization of inducer concentration (IPTG) for ß-galactosidase expression 17. Optimization of time of induction for ß-galactosidase expression   School of Biotechnology 463 . Polymerase Chain Reaction (PCR) for the given standard DNA using PCR kit and verifying with agarose gel electrophoresis 16.  6. Screening of recombinant transformants by Blue-White selection method 15.Coli 10. Extraction of proteins from plant or animal tissue and confirmation with qualitative tests 7. Transformation of recombinant plasmid DNA into competent cells 14. Western blotting for tissue extracted proteins or serum proteins separated in SDS-PAGE 9.

Convection and Radiation. SFEE. concept of work and heat transfer. dryness fraction. latent heat. Basics of Fluid mechanics and thermal flow 2. turbulent.(Simple calculations). Unit II: Introduction to thermodynamics Thermodynamic system and Control volume. Vapour pressure. Transient heat conduction – Lumped capacitance method for bodies of infinite thermal conductivity Theory of insulation. Types of fluids. Zeroth law of thermodynamics. Steady heat conduction in simple geometries – Plane wall. Unit III: Heat Transfer – Conduction Modes of heat transfer –Conduction. Equation of state-ideal gas. Bernoulli equation. pressure drops in pipes. Rotameter. surface tension. thermodynamic Properties. Develop processes with better heat efficiency and economics Unit I: Introduction to Fluid Mechanics: Fluids-Properties. (Simple problems in SFEE). sensible heat.  ADDITIONAL SUBJECTS   Code CL217 CL218 CL219 CL220 CL221 CL222 CL223 CL303 CL304 CL305 Subject Name Fundamentals of Thermal Fluid Sciences for Food Engineers Process Engineering Calculations Unit Operations in Food Processing I Unit Operations in Food Processing II Biochemical Thermodynamics Unit Operations Principle of Chemical Engineering Advanced Chemical Engineering Introduction to Biochemical Engineering Advanced Chemical Engineering Lab Credits 3:0:0 3:0:0 3:0:0 3:0:0 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 0:0:2 CL217 FUNDAMENTALS OF THERMAL FLUID SCIENCES FOR FOOD ENGINEERS Credit: 3:0:0 Objectives To enable the student to understand: 1. First law of thermodynamics –closed and open system. Venturimeter. Orificemeter. capillary effect. Importance of thermal fluid sciences in processing of food 3. Thermodynamic equilibrium. thermodynamic process. cylindrical wall without heat generation. critical radius of insulation Overall heat transfer coefficient (Derivation not required-Simple problems). Properties of steam.   School of Biotechnology 464 . Fluid flow. Pitot tube-working principles only.laminar. Second law of thermodynamics. valves and bends.

Liquids and Solids Ideal Gas relationships. “A Text book of fluid Mechanics & Hydraulic Machines”. turbulent flow over a flat plate. To understand the various food processing operations with reference to balance of materials used and energy required. 3. Robert H..K. conventions in methods of analysis and measurement. Evaluation of convection heat transfer coefficient.” Fundamentals of Thermal Fluid Science”.. To study the various units and dimensions used for measurements. Text Book: 1. Nag. Unit V: Heat Transfer with phase change: Boiling and condensation. et al. Mole unit. 2. 2. Unit I : Introduction to Food Engineering Calculations: Systems of measurements. (Simple problems). Concentration and Dehydration. Reference Books: 1. 1993. calculations of enthalpy changes. temperature. Yunus A. Laxmi Publications (1992). Heat Transfer by P. 2002. review of SI system of measurement. “A course in heat engines and thermodynamics”. basis. Vapours.. Dhanpat Rai & Sons. Forced convection inside tubes. Flow boiling.  Unit IV: Heat Transfer – Convection and Radiation: Convection heat transfer – forced and natural. heats of solution and mixing: Unsteady state material and energy balances. Pool boiling. general balance with or without reactions. R. chemical equations and stoichiometry Unit II : Gases. Tata McGraw Hill. Bansal. (Derivation not required-Simple problems).C. Tata McGraw Hill Publishing Company Ltd. CL218 PROCESS ENGINEERING CALCULATIONS Credit : 3:0:0 Objectives: 1. Kothandaraman. 3.Turner.New Delhi.Cengel. To study the basic kinetic theory during food processing.P.   School of Biotechnology 465 . New Delhi. Basics of Radiation heat transfer. Multistage processes and numerical examples Unit IV : Energy Balances Concept and units. Basic concepts multiphase flow. 3rd Edition. pressure. material balances involving condensation and vaporization. Units and Dimensions.2005.K. vapour equilibria for multi-component systems. Unit III : Material Balances Material balance of food processes involving Dilution. heat transfer coefficient for Laminar flow in a tube with constant heat flux and constant wall temperature.

To study the engineering principles involved in processing of food materials.Toledo: Fundamentals of Food Process Engineering. Single and multiple effect evaporation. L.  Unit V: Kinetics of Chemical Reactions in Foods Theory of reaction rates. and reaction rate constant. Vapour recompression. New York (1981) 3. Unit IV : Contact Equilibrium Separation Processes   School of Biotechnology 466 . Unit I : Introduction Basic principles of food processing. Thermo and mechanical systems. basic principles. 2. To understand the various unit operations in food processing. Mass transfer phenomenon applied to food systems Unit III : Evaporation Needs. temperature dependence of reaction rates Text Books 1. To study the mass transfer by gases and solvent extraction processes. New York (1991) 2. Steam distillation. types of reactions. New York CL219 UNIT OPERATIONS IN FOOD PROCESSING I Credit : 3:0:0 Objectives: 1. Clair Batty and Steven L Folkman: Food Engineering Fundamentals. and calculations involving various physical parameters.Riggs: Basic Principles and Calculations in Chemical Engineering : Prentice Hall International. Merson: Food Engineering. & J. Romeo T. enzymatic reactions. Himmelblau D.M. boiling point elevation. Unit II : Distillation Distillation. theory and examples. Calculations. London 2. applications and equipment. dimensionless ratios. J. Molecular distillation. Loncin and R. Heat economy. Principles and selected applications: Academic Press. climbing film tubular evaporators. AVI Publishing company. plate evaporators. thin film and scraped surface evaporators. Units and dimensions of engineering parameters. Van Nostrand Reinhold. 2004) Reference Books 1. 3. John Wiely & sons Publishers. (7th Edition. Falling film. B. M. Stanley E Charm: The fundamentals of Food engineering. Conservation of mass and energy.

Richardson. Saravacos: Food Process Design Marcel Dekker. Pergamen Press. Diffusion of aroma components Applications of diffusion and migration in food packaging Unit II : Mechanical Separations Filtration and sedimentation. J. Mass transfer operations. Zacharias B.Vol 2 Particle Technology and Separation Processes. AVI Publishing Company Inc. operating conditions. Solubility. Diffusion of gases in liquid and solid foods. liquid-liquid extraction. 5.Elsevier science Ltd. Gas-liquid equilibria. Supercritical Phase Equilibria. Equilibrium concentration relationships. Volatility etc. Density. extraction of Fatty acids.L. Develop processes with better efficiency and economics Unit I : Adsorption and Diffusion Basics of adsorption. Marcus Karel & Deryl B Lund: Physical Principles of Food Processing. Relative advantages. Applications. Importance separation processes in designing a process 3. Inter-phase moisture transport. Boston. D. J. J. Maroulis and G. Albert Ebarz: Unit Operations in food Processing. Hops.F. Basics of Solid-liquid extraction processes. Harker .  Concentrations. Dennis R. New York Reference Books 1. CRC Press 2. Moisture transfer in foods.H. Essential oils and Bio chemicals.Coulson & Richardson's Chemical Engineering . Examples Industrial applications and equipments for Filtration and Sedimentation   School of Biotechnology 467 . Basics of mass transfer separations 2. Extraction. Fifth Edition.Earle: Unit Operations in Food Processing.Lenigar and W. USA (2003) CL220 UNIT OPERATIONS IN FOOD PROCESSING II Credit : 3:0:0 Objectives To enable the student to understand: 1. H. 3. limitations and economics Text Books 1.Reidal Publishing Co. Diffusion in porous foods. Marcel Dekker Inc.Beverlo: Food Process Engineering.S. Viscosity. CRC Press Ltd.M. 2. gravity sedimentation. SCFE systems and components. Coulson. USA 4. D. with food application examples. (II Edition or later). (1981) 6. H. Heldman and R. Markotte: Food Processing Principles and Applications. 2002. Butterworth & Heinemann . Ramaswamy & M. R. Properties of Super critical fluids. Paul singh: Food Process Engineering (II Edition). Solid liquid equilibria.A. Crystallization Unit V : Super Critical Fluid Extraction Super critical Fluid State.A. Sedimentation of solid particles in gas media.

Applications in food industry.D. Rielly: Chemical engineering for the Food Industry. feed and discharge arrangement in each case. R. 2. Hong Kong.F. Different types of centrifuges – Basket. D. Importance of balancing of rotating masses. Tubular Bowl.L. Nozzle centrifuges. Extrusion cooking. Elsevier Publishing . Steam Cookers. Configuration of membranes.J.H. Particle Technology and Separation Processes. Nano filtration (NF). Blackie Academic and Professional. Mcgraw Hill New York 6.  Unit III : Centrifugal Separation Sedimentation and filtration centrifuges.Heid: Food Processing Operations (3 Volumes) AVI Publishing company Inc. A.S. E. Basic equations. Different kinds of Roasters. (II Edition or later).Vol 2. Elsevier Publishing 5. relative advantages and limitations. Unit IV : Membrane Filtration Definitions. Jimmy L. Richardson. Specific characteristics. D. Butters. Cross flow filtration. Decanting. Reverse Osmosis (RO). Humphry and G. Marcel Dekker Inc. 3rd Edition.Lewis: Separation processes in the Food and Biotechnology industries (Principles and applications): Woodhead publishing Ltd. Disk bowl.J. Fifth Edition. R. London.Wilke: Extraction with Supercritical Gases. 2002. Membranes and their characteristics. Text Books 1. J. E. Desludging (Self Cleaning) Bowl Centrifuges. Keller: Separation Process Technology. USA 1986 CL221 BIOCHEMICAL THERMODYNAMICS Credit : 4:0:0 Unit –I: Basic Concepts in Engineering Thermodynamics   School of Biotechnology 468 .Grandison and M. Stahl and G. Unit V : Roasting and Cooking Theory. advantages and applications. Advantages of Extrusion cooking over conventional cooking. Brennen: Food Engineering Operations (Vol 1 and 2).M. membrane materials. Harker . Reference Books 1.Schneider. and A. E.Earle: Unit Operations in Food Processing.H.L. J. Single and Twin Screw Extruders. 1990 3. J. Butterworth & Heinemann . N. Cowell. Cambridge UK 7.L. Ultra filtration (UF) and Micro filtration (MF). Coulson. Maynord Joslyn and J. Pyle and C.Elsevier science Ltd. G. Rakesh Singh and S. Lilley: Food Engineering Operations (Vol 1 and 2). V. Chennai 4. New York. 3. Verlag Chemie publishing.M. Basel 2. J. Molecular weight cut off in each case. Pumps and other membrane equipment.Rizwi: Bio Separation Process in Foods. Fryer.Coulson & Richardson's Chemical Engineering. F. Pergamen Press. Forming extruders and snack food extruders.

Abbott. 1999.V.  First and Second law of Thermodynamics. Calculation of equilibrium conversions and yields for single and multiple chemical reactions. H. 2002. equilibrium criteria for homogeneous chemical reaction.M. Unit – V: Biochemical Thermodynamics Energetic of Metabolic path ways. Concept of heat transfer by convection. yield coefficients.Smith.C. transducer combinations. Y. Biosensor development and diversification. Thermodynamics of flow process. Thermodynamics efficiency of growth. Unit –II: Thermodynamics Properties of Fluids Volumetric properties of gases exhibiting non-ideal behavior. 3. Types of convection. Liquid. Hollow spheres and hollow cylinder. Unit – II. Thermal conductivity. mean temperatures.M. Unit – IV: Biosensors Definitions. Unit –III: Phase and chemical reaction Equilibrium Criteria for phase equilibria. Calculation of Work. Oxygen consumption and heat evolution in aerobic cultures. Reference Books: 1. Elsevier. Thermodynamics.A. application of dimensional   School of Biotechnology 469 .C. Practical forms of Biosensors.steady state – unsteady state heat conduction. Stoichiometry and energetic analysis of cell Growth and PRODUCT FORMATION. specific applications of biosensors. Estimation of Thermodynamics properties using equations of state. 1983 CL222 UNIT OPERATIONS Credits: 4:0:0 Unit I Heat Transfer. Energy Coupling (ATP& NADH). Fourier’s law of heat conduction. Van Ness and M. Convection and Radiations. Rao. Maxwell relationship and their applications. Chemical Engg. Degree of reduction concepts available – electron balances. Basic considerations and conduction Importance of heat transfer in chemical and biotech operations . modes of heat transfer. J. Roels. Sixth edition. Elemental Balances. Direct Electron transfer biosensors and Optic biosensors. University press.Liquid equilibria and Solid – liquid equilibria . energy and property changes in reversible process. McGraw Hill. Calculation of flow based on actual property changes. J. Evaluation of equilibrium constant and effect of pressure and temperature on equilibrium constant. Vapour – liquid equilibrium calculations for binary mixtures. Conducto-metric biosensors. 2. Power cycle and refrigeration cycles. Residual properties. individual and overall heat transfer coefficients.Concept of heat conduction. Heat conduction through the composite wall. immobilization and membranes. Kinetic and Energetic in Biotechnology. Introduction to Chemical Engineering Thermodynamics.

power for agitation. Types of heat exchanger .1993. Molarity. Fundamentals Of Heat And Mass Transfer. limiting and excess reactant. Concept of Simpson rules and its application Unit – II. Filter aids – Filter theory .  analysis for convection: Derive the equation for laminar . Unit Operations In Chemical Engineering. Fouling factor . weight fraction.   School of Biotechnology 470 . Steam economy. Calculations of Material and Energy balances . Incropera F. Centrifugal and centrifuge. Credits: 4:0:0 Unit-I Importance of units and Basic Calculation. Viscous material and pastes.Atomic.L. LMTD.evaporation. molality and normality. specific gravity. mixing of Powder.Laws of radiations.meaning of material balance and its applications-like distillation . agitation of liquids. temperature.Concept of Radiation.2002. Visual Density.P. problems. Types of feeding.1998 CL223 PRINCIPLE OF CHEMICAL ENGINEERING. Geankoplis C. application of LMTD. Types of filter – Constant pressure filtration. mole fraction. gas-liquid systems. gas-solid suspensions. Transition and Turbulent conditions.density. Agitator scale up. 2. Mixing and Agitation Dimensional analysis.how to prevent the dirt factor. crystallization.Meaning of energy balance and its applications . selection of medium. free settling and Hindered settling.Types of evaporators.5th Edition.Mcgrawhill. Unit – V. Prentice Hall India. Transport Processes And Unit Operations.C. Batch sedimentation. Sedimentation. Ideal gas law-Ideal mixtures and solution-Dalton law of additive volumes. Heat transfer from condensing Vapours.. Conservation of energy. design of heat exchanger. Heat transfer to boiling liquids. recycle . mixtures and solutions. John Wiley. bypass and purging. Emulsifying – rpm.Evaporator.J. Reference 1.moles. McCabe W. Material Balance with and without chemical reaction Laws of conservation of mass. Smith J. constant volume filtration. drying etc Material balance with Chemical reactions.Types of filtration . Text Books 1. Stabilizers. Grey and Black bodies.parallel and counter flow heat exchanger .concept of Evaporator capacity. Particle Size. Filter media . Conversion factors. Unit-III.Molar concept. Reduction. molecular & equivalent weights. Stability. Gear motor system.Industrial filtrations. Heat transfer in packed and fluidized beds. Unit-IV. Mechanical separation Filtration . Heat Exchanger Heat exchanger – Types of flow.

  School of Biotechnology 471 . Text Books: 1.pressure measurementsDimensionless analysis and similitude. Nature of fluids .Velocity potential . 2. globe and ball valve. butterfly valve. Fluidization.Pump Characteristics. Various types of pumps. fans and blowers. Chemical process calculation –by Ghavene – Delhi publications. CL303 ADVANCED CHEMICAL ENGINEERING. velocity profile and friction factor for smooth and rough surface pipes. molality and normality-Ideal gas law-Ideal mixtures.. Unit operation of Chemical Engg “ Chattopathya” Khanna publication. Measurements of fluid flow and flow controls.Hill.gate valve . Credits: 4:0:0 Unit-I Importance of units and Basic calculation. pitot tube.properties of fluids. Transportation of Fluid Transportation of fluids – fluids moving machinery performance.continuity And mechanical energy equations. weight fraction. 2. fluid static . wires and notches. Unit. Transportation of fluids. Friction factor for packed beds. needle valve . centrifugal pumps. Air lift and diaphragm pumps positive displacement pumps.Types of fluids. Ergun equations. venturimeter.. BI Bhatt &SM vora “ Stoichiometry” Tata Mcgraw. Fluid mechanics. Conversion factors-Molar concept-moles. Fourth Edition.Concept of Simpson rules and its application. types.Tata McGraw hill – 7 edition. Transport Process and Unit operations-“Geankoplis” Prentice – Hall. mixtures and solutions. reciprocating pumps.Flow controls . Rota meter.  Unit. Selection and specification.Compressors and Blowers.Types of fluids. its application.III. Head losses for various stations.pressure measurementsDimensionless analysis and similitude-. fluid static’s.V. Fluid flow measurement Measurements of fluid flow – orifice meter.Harriot. pump characteristics. Unit Operation of Chemical Engineering by McCabe and Smith. UNIT –IV. Conservation of energy-Meaning of energy balance and its applications Unit – II Fluid mechanics. Reference: 1.properties of fluids. Concepts of compressors.volume -1. Laws of conservation of mass-its applications .Molarity.mechanism . 3. Nature of fluids . mole fraction. Unit-III Membrane Separation.

degrees of reduction of   School of Biotechnology 472 . Unit-V Mechanical Separation and mixing. centrifugal and centrifuge concept of mixing and agitations. Flow Measuring Devices. Basic design and construction of a Fermentor and its ancillaries. metabolic heat generation. CL304 INTRODUCTION TO BIOCHEMICAL ENGINEERING Credit: 4:0:0 Unit I Introduction and overview of Fermentation Processes Introduction. batch sedimentation. agitator scale up. Unit Operation of Chemical Engineering by McCabe and Smith. filter aids. available – electron balances. 2. fouling factor. cell growth and product formation. Biotechnology and engineering. Unit operation of Chemical Engg “ Chattopathya” Khanna publication. Chemical process calculation –by Ghavene – Delhi publications. Fourier’s law of heat conduction. Unit III Metabolic Stoichiometry and Bioenergetics Thermodynamics. Thermal death kinetics of microorganisms.Hill.General requirements of Industry. need for Biochemical engineering. Reference: 1.volume -1. Text Books: 1. Unit-IV Heat Transfer: Basic concept of heat transfer . examples of simple and complex media.. Vessel Geometry. Types of Flow and LMTD. Fourth Edition. types of filtration. Transport Process and Unit operations-“Geankoplis” Prentice – Hall.Tata McGraw hill – 7 edition. Filter Sterilization of liquid Media and air sterilization. industrial filtrations. Heat exchanger. 2. 3. radiation and chemical sterilization. Sedimentation. Batch and continuous Heat.  Membrane Separation process: Solid and Liquid membrane : Concept of Osmosis: Reverse osmosis: Electrodialysis: Their applications: Foam Separation process: Thermal and sweep diffusion process. Filtration . Concept of convection and radiation.Harriot. mass and energy balances in microbial metabolism. mode of heat transfer. valves (Basic features) Biochemical fermentation Materials of construction Unit II Media Design and Sterilization for Fermentation processes Medium requirements for fermentation processes.Sterilization of Liquid Media. Stoichiometry of Cell growth and product formation . Filter medium . BI Bhatt &SM vora “ Stoichiometry” Tata Mcgraw. Design and usage of commercial media for industrial fermentations. mixing power calculation –high viscous materials and paste . construction. History of Biochemical engineering. Sterilization equipment – batch and continuous. Design of exchanger.elemental balances.

Tata – McGraw Hill. scale. yield coefficients of biomass and product formation. thermal conductivity   School of Biotechnology 473 . 2. Unit V Process design and operation of Bioreactors Operational modes of reactors – batch. Biochemical Engineering Fundamentals. Text Books: 1. continuous.). Academic Press.). Bioprocess engineering. Atkinson. fluidized bed column 9. 3. Shule and Kargi. 1995 CL305 ADVANCED CHEMICAL ENGINEERING LAB Credit: 0:0:2 1. 3. oxygen consumption and heat evolution in aerobic cultures. D. Bioreaction Engineering (Volume 1). Bailey J. Biochemical Engineering and Biotechnology Handbook. reactors for waste – treatment processes. Karl Schugerl. oxygen uptake rates and determination of oxygen transfer coefficients (kla).. Biotechnology(Vol. Unit IV Transport phenomena in bioreactors Mass transfer in heterogeneous biochemical reaction system: Oxygen transfer in submerged fermentation processes.K.F.E. T. maintenance coefficients. role of aeration and agitation in oxygen transfer. plate type heat exchanger 4. packed bed column 10. Biochemical Engineering. orifice meter 8.  substrate and biomass. 4. Prentice Hall. Bioprocess Engineering Calculation. References: 1. Heat transfer processes in biological system. F. packed bed distillation 6. Clark. Pauline Doran. Process Computations in Biotechnology. 1(1994). B. H. ventur meter 7. novel bioreactors: Stirred tank. packed – bed and hollow – fibre membrane bioreactors. & Mavituna. Double pipe heat exchanger 2. screening analysis 5. Harvey W. shell and tube heat exchanger 3.up criteria for bioreactors. McGraw Hill. Bioprocessing). (1986). VCH (1993) 5. Blanch and Duoglas S.(1997) 6. and Ollis. Reed (Ed. air lift and loop reactor. John Wiley (1987). Ghose (Ed. repetitive batch. recycle and continuous cultivation. Rehm and G. fed batch.J. heat loss due to contraction and expansion 11. Marcel Dekker Inc. 2. McGraw Hill (2nd Edition) (1993). Second Indian Reprint (2004).

School of Biotechnology and Health Sciences Department of Biotechnology (BT) SCHOOL OF BIOTECHNOLOGY AND HEALTH SCIENCES Karunya University  Page 615  .

IPR and Biosafety Basics of Bioinformatics Bioinformatics Lab Chemical Thermodynamics and Bio Thermodynamics Bio Reaction Engineering Advanced Bioprocess Lab Protein Engineering Plant Biotechnology Credits 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 0:0:2 4:0:0 4:0:0 4:0:0 0:0:2 4:0:0 4:0:0 4:0:0 0:0:2 4:0:0 4:0:0 0:0:2 4:0:0 0:0:2 4:0:0 0:0:2 4:0:0 4:0:0 4:0:0 0:0:2 4:0:0 4:0:0 4:0:0 4:0:0 0:0:2 4:0:0 4:0:0 0:0:2 4:0:0 4:0:0 Page 616  .School of Biotechnology and Health Sciences Department of Biotechnology (BT) ADDITIONAL SUBJECTS Subject Code BT335 BT336 BT337 BT338 BT339 BT340 BT341 BT342 BT343 BT344 09BT201 09BT202 09BT203 09BT204 09BT205 09BT206 09BT207 09BT208 09BT209 09BT210 09BT211 09BT212 09BT213 09BT214 09BT215 09BT216 09BT217 09BT218 09BT219 09BT220 09BT221 09BT222 09BT223 09BT224 09BT225 09BT226 09BT227 09BT228 09BT229 09BT230 09BT231 09BT232 09BT233 Karunya University  Subject Name Advanced Reaction Engineering Modern Separation Processes Energy Management Unit Operations and Processes in Effluent Treatment Bio-Plant Design and Practice Biological Wastewater Treatment Molecular and Cellular Basis of Cancer Advanced Biopharmaceutical Technology Phytochemicals and Herbal Medicine Advanced Plant Biotechnology Basic Industrial Biotechnology Instrumental Methods of Analysis Principles of Chemical Engineering Chemical Engineering Lab Unit Operations Mass Transfer Operations Biochemistry Analytical Biochemistry Lab Bio Organic Chemistry Cell Biology Microbiology Microbiology Lab Molecular Biology Genetic Engineering Molecular Biology & Genetic Engineering Lab Immunology & Immunotechnology Cell Biology and Immunology lab Bioprocess Principles Bioprocess Lab Enzyme Engineering & Technology Bioprocess Engineering Downstream Processing Downstream Processing Lab Metabolic Engineering Applied Plant & Animal Biotechnology Bioethics.

School of Biotechnology and Health Sciences Department of Biotechnology (BT) 09BT234 09BT235 09BT236 09BT237 09BT238 09BT239 09BT240 09BT241 09BT242 09BT243 09BT244 09BT245 09BT246 09BT247 09BT248 09BT249 09BT250 09BT251 09BT252 09BT253 09BT254 09BT255 09BT256 09BT257 09BT258 09BT301 09BT302 09BT303 09BT304 09BT305 09BT306 09BT307 09BT308 09BT309 09BT310 09BT311 09BT312 09BT313 09BT314 09BT315 09BT316 09BT317 09BT318 09BT319 09BT320 09BT321 Karunya University  Animal Biotechnology Cancer Biology Bioprocess Economics and Plant Design Applied Environmental Biotechnology Introductory Genomics and Proteomics Bio Pharmaceutical technology Nano Biotechnology Analytical Techniques in Biotechnology Lab Introductory Biotechnology Applied Microbiology Introductory Cell Biology Introductory Genetic Engineering Role of Biotechnology in Environment Basics of Biomolecules Gene and Gene Expression Introductory Microbial Biotechnology Applied Biotechnology Instrumental Methods of Analysis Nanobiotechnology Cancer Biology Basic Research Methodology Animal and Plant Tissue Culture Lab Industrial Safety Renewable Energy System Bioremediation for Industrial Sectors Cell Biology and Advanced Biochemistry Cell Biology and Advanced Biochemistry Lab Biochemical Engineering Chemical and Biochemical Engineering Lab Advances in Genetic Engineering Genetic Engineering Lab Advanced Bioprocess Engineering Bioprocess Engineering and Downstream processing Lab Metabolic Regulations and Engineering Advanced Immunotechnology Immunotechnology Lab Advanced Biopharmaceutical Technology Downstream Processing in Biotechnology Analytical Biotechnology Lab Research Methodology Instrumentation and Biotechniques Advanced Reaction Engineering Modern Seperation Processes Energy Management Unit Operations and Processes in Effluent Treatment Bio-Plant Design and Practice 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 0:0:2 3:0:0 3:0:0 3:0:0 3:0:0 3:0:0 3:0:0 3:0:0 3:0:0 3:0:0 3:0:0 3:0:0 3:0:0 2:0:0 0:0:2 3:0:0 3:0:0 3:0:0 4:0:0 0:0:2 4:0:0 0:0:2 4:0:0 0:0:2 4:0:0 0:0:2 4:0:0 4:0:0 0:0:2 4:0:0 4:0:0 0:0:2 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 Page 617  .

School of Biotechnology and Health Sciences Department of Biotechnology (BT) 09BT322 09BT323 09BT324 09BT325 09BT326 09BT327 09BT328 Biological Waste Water Treatment Molecular and Cellular Basis of Cancer Phytochemicals and Herbal Medicine Advanced plant Biotechnology Basics of Chemical Engineering (Bridge course) Research Methodology Animal and Plant Tissue Culture Lab 4:0:0 4:0:0 4:0:0 4:0:0 3:0:0 3:0:0 0:0:2 Karunya University  Page 618  .

spiral wound and hollow fiber membrane reactors. Microfiltration and Donnan dialysis. Commercial. J. Karunya University  Page 619  . Unit III Internal Transport Processes in Porous Catalysts Inter pellet mass and heat transfer. Recent advances in separation techniques based on size. Unit IV Analysis and Design of Heterogeneous Reactors Isothermal and adiabatic fixed bed reactors. Enzyme fermentation. BT336 MODERN SEPARATION PROCESSES Credit : 4:0:0 Unit I Review of conventional processes. Unit-V Bio Chemical Reaction system.School of Biotechnology and Health Sciences Department of Biotechnology (BT) BT335 ADVANCED REACTION ENGINEERING Credit : 4:0:0 Unit I Kinetics of Heterogeneous Reactions Catalytic reactions. 1997. Froment. dual functional filter. Carberry. Chemical reaction engineering 3rd Edition. shrinking and unreacted core model. quantitative treatment of external transport effects. steady state approximation. Two – phase fluidized bed model. microbial fermentation introduction and overall picture. Ceramic membranes. Surface based solid-liquid separations involving a second liquid. John Wiley & Sons. Unit II Types and choice of membranes. pilot plant and laboratory membrane permeators involving dialysis. John Wiley & Sons. mass and heat transfer with reaction. Octave Levenspial. reverse osmosis. 1976. and Bischoff. product limiting microbial fermentation References: 1. 2nd Edition. 3. cross flow electro filtration. New York. non-isothermal and non-adiabatic fixed bed reactors. Langmuir-Hinshelwood model. Chemical Reactor Design and Analysis. Rideal-Eiley mechanism.F. G. tubular. 2. Biological reactions in fermentation processes Unit II External Diffusion Effects in Heterogeneous Reactions Mass and heat transfer coefficients in packed beds.B. rate controlling steps. ultrafiltration. non-catalytic fluid-solid reactions. Economics of membrane operations. K. Nanofiltration. slurry reactor model. evaluation of effectiveness factor. McGraw Hill. and trickle bed reactor model. centrifugal separators and their relative merits. ionic properties and other special characteristics of substances. modeling diffusion with and without reaction. 1999. substance – limiting microbial fermentation. Plate and frame.. New York. Experimental determination and evaluation of reaction kinetics for heterogeneous systems. process concept. New York.J. Chemical and Catalytic Reaction Engineering. Theory and equipment used in cross flow filtration. surface properties..

nuclear energy. commercial generation of power requirements and benefit. society and environment population and technology. 1982. Types of equipment and commercial process. liquids and gases.. Unit V Separations involving lyophilisation..R. various forms. supply and demand. need for conservation. Roussel. depletion of resources. energy storage. Commercial processes. Normal adsorption techniques. Classification. fermentation. Zone melting. Pervaporation and permeation techniques for solids. Unit IV Controlling factors. Recent advances and process economics. photosynthesis-Global warming Unit II Forecasting techniques. BT337 ENERGY MANAGEMENT Credit : 4:0:0 Unit I Energy sources. Industrial effluent treatment by modern techniques. Affinity chromatography and immuno Chromatography. References: 1. 1987. growth and change. Tata McGraw Hill. Separation Processes. 3. patterns of consumption in developing and advances countries. Supercritical fluid extraction. cooling tower. energy demand. Unit V Karunya University  Page 620  . C. food industries. hydroelectricity. conservation in unit operation such as separation. O. input and output analysis. pharmaceutical industries. ion exchange chromatography and electro dialysis. coal oil. pulp and paper. Energy and evolution. King. Types of equipment employed for electrophoresis. Adductive crystallization. Types and choice of adsorbents.W. other fossil fuels. Nakagawal. 2.V. uncertainties. New York. Unit III Bio-geo-chemical cycles. Handbook of Separation Process Technology. magnitude and pattern. drying.J. Energy. Oil spill Management . energy modeling and optimal mix of energy sources. Marcel Dekker. Industrial viability and examples. cement. Dielectrophoresis. John Wiley. geothermal. national and international issues-Ozone layer depletion. natural gas. Membrane Science and Technology. Other separation processes. 1992. Applications. Unit IV Chemical industries. conservation applied to Bio-fertilizers.School of Biotechnology and Health Sciences Department of Biotechnology (BT) Unit III Mechanisms. structural properties of environment.. conservation using optimization techniques.

3. Energy.equation. Solid waste management in Biochemical industries Unit III Filtration – classification of filters – Head loss through filters – Darcy . Unit IV Chemical precipitation – phosphate removal – Adsorption – Activated carbon – isotherms – Disinfection – Factors influencing – Breakpoint chlorination – Dechlorination.. McGraw-Hill Book Co.Screening – Mixing – Coagulation and Flocculation – Flow equalization. 1976.H. 2. G. Krentz. Cost analysis. wind . METCALF & EDDY..School of Biotechnology and Health Sciences Department of Biotechnology (BT) Sources of continuous power. input-output analysis. 1993 BT339 BIO-PLANT DESIGN AND PRACTICE Credit : 4:0:0 Unit I Plant Design Fomenter design. production rate.Principal type of Reactors. 2. Unit II Sedimentation – Type of settling – Removal ratio – Clarifier – thickener – Column flotation – air floatation. Bio-fuel.. References: 1. reactions vessels. Allyn and Bacur Inc. tariffs.J. Macmillan Publishing Co. economics. geothermal. Elements of Energy Conservation. production analysis and production using fuel inventories. 1975. 1985. fuel cells. and Reuse “. Tata McGraw-Hill. Unit II Process economics Karunya University  Page 621  . “Wastewater Engineering–Treatment. J. Energy conservation and utilization. New York. corporate models. Unit V Kinetics of biological growth – Suspended and attached growth processes – Aerobic and Anaerobic – Determination of kinetic coefficients. MHD. Materials of construction and properties. Casey. Pressure relief system.M. 1995. Third Edition.”Unit Treatment Processes in Water and Wastewater Engineering”. Disposal. tidal and solar power. system rate. References: 1.. John Wiley & Sons. BT338 UNIT OPERATIONS AND PROCESSES IN EFFLUENT TREATMENT Credit : 4:0:0 Unit I Selection of unit operations and processes. capacity. piping and valves for biotechnology. Gramlay. Utilities for plant design.K. system cost analysis.. T.. C. Energy generation from Bio-wastes. INC. Rused.Biogas-pyrolysis .

Biological Nutrient Removal.K.. 2. “ Plant Design and Economics for Chemical Engineers “. Two case studies – one traditional product and one recombinant product. Reactor Receiving Soluble Substrate. self inspection. and Timmerhaus. Multiple Microbial Activities in a Single Continuous Stirred Tank Reactor. Anaerobic Processes. when does validation occur. 5th Edition. Multiple Microbial Activities in Complex Systems.. modeling of ideal suspended Growth Reactors. References: 1. Springer. contract manufacturing and analysis. BIOTOL Series. Resources for validation. complaints and product recall. Activated Sludge. 4. Aerobic Growth of Heterotrophs in a single Continuous Stirred Tank. Documentation.JohnWiley. food and pharma industries Unit IV Validation of Biopharmaceutical Facilities Introduction. Unit III Pharmaceutical water system Grades of water. Seiler. personal. The why and How ?”. Peter.1993 3. B. Why Validation. Unit II Theory. 2001. validation of Systems And Processes including Sip And Cip. validationSanitary design principles : Biochemical. Lagoons. quality control. water distribution system. production cost estimate. etal.School of Biotechnology and Health Sciences Department of Biotechnology (BT) General fermentation process economics. “A Compendium of Good Practices in Biotechnology “. Modeling Suspended Growth Systems. water treatment system.. Equipment and Facilities”.1994. Unit III Applications: Suspended Growth Reactors.Jiing P. Unit IV Karunya University  Page 622  . Design and Evaluation of Suspended Growth Processes.1997. BT340 BIOLOGICAL WASTEWATER TREATMENT Credit : 4:0:0 Unit I Classification of Biochemical operations. McGraw Hill. Lydersen. “Good Laboratory Practice. “Bioprocess Engineering Systems. Pharma and Biotech industries. materials usage and cost. Stoichiometry and Kinetics of Biochemical Operations. capital investment estimate.Hazardous analysis: Food. Klaus D. production. Unit V Good manufacturing Practices Structure – quality management. fundamentals of biochemical operations. sanitary design. Max S. Validation Structure. Techniques for Evaluating Kinetics and Stoichiometric parameters. Introduction to GLP and its principles. Aerobic – digestion. ButterworthHeiemann. premises and equipment.

DNA methylation and epigenetic silencing of suppressor genes. UNIT IV Understanding Natural History of Cancer Development Free radicals. Growth factor receptor kinases and cytoplasmic tyrosine kinases. Cyclic AMP and activation of CREB. Submerged Attached Growth Bioreactors. Rotating Biological Contactor. Cancer immunity and strategies of anticancer immunotherapy. BT341 MOLECULAR AND CELLULAR BASIS OF CANCER Credit: 4:0:0 UNIT I Cancer Biology: the Basics Introduction. Tumour suppressor gene pathways. Mizahi A. radiation therapy. Bio-film Modeling. diet and cancer. Telomerases UNIT III Oncogenes and Tumour Suppressor Genes Apoptosis. Aerobic Growth of Heterotrophs in Rotating Disc Reactors. cell adhesion. & progression. Carcinogenesis: Theory of carcinogenesis. Metabolic oxidative stress and cancer. promotion. Retino Blastoma tumour suppressor genes and its significance. Graty. Trickling Filter. Genetic changes of selected cancers – Lung cancer. Immunomodulators in cancer therapy. growth factor receptors and Signal transduction. metabolism of carcinogenesis. Growth factors. invasion and metastasis. Natural products and cancer. Chemical carcinogenesis. Tumour supressor genes. Gastrointestinal cancers and other cancers. X-ray and UV radiation-mechanisms of radiation carcinogenesis.C. Cancer initiation. Breast cancer. principles of physical carcinogenesis. Cellcell interactions. Retroviral oncogenes.L. Aerobic Growth of Biomass in Packed Towers. angiogenesis. G and Lim. JAK-STAT pathway. Fluidized Bed Biological Reactors. References 1. Ras/MAPK pathway. Cellular proto-oncogenes and oncogene activation. 1998 2. Biological Wastewater Treatment. Tumour markers UNIT II Cell cycle regulation and Signal Transduction Cancer cell cycles. Future Challenges. Transcription factors. Tumour associated antigens and Tumour specific antigens UNIT V Current Concepts in Cancer Therapy Different forms of therapy – Chemotherapy. antioxidants and cancer. John Wiley Sons Inc 1989. surgery. Biological Waste Treatment. Fate and Effects of Xenobiotic Organic Chemicals. Unit V Applications: Attached Growth Reactors. Karunya University  Page 623  . Cell cycle control genes and mismatch repair genes. Gene therapy and cancer. 2nd Edition.P. C. Applications of Monoclonal antibodies in cancer diagnosis and therapy.Daigger. Marcel Dekker. H. screening and early detection.School of Biotechnology and Health Sciences Department of Biotechnology (BT) Theory: Modeling of Ideal Attached Growth Reactors. historical perspective and classification of cancer.

The Molecular Biology of Cancer. Biotechnology production of Secondary Metabolites UNIT. Blackwell Publishers. Purohit. Oral liquids. Bios Scientific Publishers. Drug delivery system – Formulation of proteins and peptides. Molecular Biology of Cancer. Interferons. Excretion of drugs.Mechanism of Drug Absorption. Radio immuno assay.School of Biotechnology and Health Sciences Department of Biotechnology (BT) Text Books 1.transdermal. Stella Pelengaris and Michael Khan.I FUNDAMENTALS OF PHARMACY PRACTICE Pharmaceutical Biotechnology: An introduction. Methods of Gene transfer.II PHARMACOKINETICS AND PHARMACODYNAMICS ADME properties. Capsules. Parchment. 3. Microbes in Pharmaceutical industry. Jodhpur. 2003. Nuclear magnetic resonance spectroscopy. 2006. Robert G. Aerosols. Macdonald F and Ford CHJ. Emulsions. Distribution of drugs. Gene Therapy. Electron spin resonance spectroscopy. 1997. Pharmacokinetics: Basic considerations. Karunya University  Page 624  . Infrared spectrophotometry. Scope & Importance of Biotechnology.liposomes UNIT IV PHARMACEUTICAL ANALYTICAL TECHNIQUES UV-Visible spectroscopy. Ointments. Alan O. somatostatin. human growth hormone.III PHARMACEUTICAL DOSAGE AND DRUG DELIVERY SYSTEM Materials & Formulations. Fluorimetry. Perantoni. somatotropin. Chromatographic techniques. Recombinant Human Deoxyribonuclease TEXT BOOKS 1. Drug metabolism (Biotransformation of drugs). Mechanism of drug action. Barry Pierce. Manufacture of Tablets. McKinnell. New York 2003. Protein as drug delivery system. Cambridge University Press. Pharmaceutical Biotechnology by S. Electrophoresis. Suppositories. Mass spectroscopy.K. The Biological Basis of Cancer. their applications. purification. Production of Human insulin. H. Controlled drug delivery system. Kakrani and A. aluja. 2. X-ray diffraction methods. UNIT. Flame emission spectroscopy and atomic absorption Spectroscopy. Novel drug delivery system. Ralph E. vaccines.S.establishing safety & efficacy. Monoclonal Antibody Based Pharmaceuticals. Sustained Release dosage forms. G. characterisation & analysis. UNIT V BIOLOGICAL PRODUCTS Properties of biotechnology derived therapeutic products. Origin & definition. BT342 ADVANCED BIOPHARMACEUTICAL TECHNOLOGY Credit : 4:0:0 UNIT.N. Parenteral solutions. Agrobios.

Indian System of medicine: Ayurveda. Preliminary screening. Biopesticides. B. Nutraceuticals and their significance. Volatile Oils .extraction methods (Taxus.. Assay of Drugs – Biological evaluation / assays. Industrial Pharmaceutical Biotechnology . edited by Michael J. N. 4th Ed. Biofungicides. Willard. Microscopic. Instrumental Methods of Chemical Analysis – B. Popovich. Rauwolfia serpentina. 4. Pharmacological).extraction methods (Clove. Biogenesis of Phytopharmaceuticals. Siddha and Unani and its significance Unit II: Medicinal & Aromatic Plants Cultivation and Utilization of Medicinal & Aromatic Plants in India. REFERENCES 1. Cinchona). Dioscorea. Nirali Prakashan. Pharmaceutical Dosage Forms and Drug Delivery Systems by Ansel. Karunya University  Page 625  . (7 edition). 7th Edition. Unit IV: Analysis of Phytochemicals Methods of Drug evaluation (Morphological.9th Edition. Kokate. Unit V:Applications of Phytochemicals Application of phytochemicals in industry and healthcare.extraction methods. Flavonoidsextraction methods. 2002 3. Antiinflammatory. K. Classification (Taxonomical. Mentha). L. Dioscorea sp.1999. Glycosides . Text Books: 1. Types of Phytochemicals: Carbohydrates & derived products. Modern Biotechnological tools and its influence in Medical and Aromatic plant cultivation. Andrographis paniculata. Wiley-VCH. Cultivation. Collection & processing of Crude Drugs. Allen. H. Plant Tissue Culture for enhancing secondary metabolite production (Withania somnifera. Catheranthus roseus. Physical & Chemical). A. Tannins (Hydrolysable & Condensed types). H. Sharma . Biopharmaceutics and Pharmacokinetics-A Treatise by DM Brahmankar. Aloe. Antidiabetic. Lectins. Sunil B Jaiswal 3. Secondary metabolite production in plants.).). Unit III: Tissue Culture of medicinal Plants Plant Tissue Culture as source of medicines. C. Morphological Chemical. K. Alkaloids . Pharmaceutical Biotechnology Concepts and Applications by Gary Walsh 2. Resins. Williams & Wilkins. Groves.. P. Analgesicdrugs. Second Edition 4. Anticancer. Microbiological methods. th BT343 PHYTOCHEMICALS AND HERBAL MEDICINE Credit : 4:0:0 Unit I: Crude Drugs Crude Drugs – Scope & Importance. Pharmacognosy. Papaver. Klefenz. Gokhale (1996).School of Biotechnology and Health Sciences Department of Biotechnology (BT) 2. Pharmaceutical Biotechnology. Biocides. Characterization of drugs. Instrumental Methods of Analysis – Hobert H. Genetics as applied to Medicinal herbs. Purohit & S.extraction methods (Digitalis.

U. 2.L. UNIT-V: Application of plant transformation for productivity and performance: herbicide resistance. functional sequencing of cDNA Expression libraries. Plant molecular genetics by Monica.Converting mRNA transcripts into cDNA libraries. References 1. use of ACC synthase. Dubay. RNA). (1989).C. Introduction to Plant Biotechnology by H. microinjection. Cultivation & Processing of Medicinal Plants. 3. sufonyl urea. Natural Products in medicine: A Biosynthetic approach (1997). atrazine. Hornok. Chawla. Mickee R. PCR. Edible vaccines. Physical method of gene transfer. K:J. PR proteins.A. nucleocapsid gene. blue rose. J. 2. Cold Spring Harbor Laboratory Press. Northern and western blotting. New Delhi. disease resistance.H. UNIT-III: Methods of gene transfer in plants: transient and stable gene transformation. use of Ti and Ri as vectors. antifungal proteins. 2001. London 6. insect resistance.uses of restriction enzymes in plant genetic engineering. Bt genes. Pharmacognosy – William Charles Evans. (ed. Oxford and IBH P Publishing Co. England. Wiley & Sons. polygalacturanase. Molecular Cloning: a Laboratory Manual. Golden rice. radiolabling and alternatives.) (1992). 2001 4. Pulsed field electrophoresis. Fritsch and T. . ADP glucose pyrophosphatase. binary vectors. UNIT-IV: Plant transformation technology: Agrobacterium tumefaciens as plant genetic engineer: features of Ti and Ri plasmids. 14th ed. Ltd.. New York. 1-3 beta glucanase. Karunya University  Page 626  . virus resistance. Maniatis. carbohydrate composition and storage. An introduction to genetic engineering in plants. 2002. viral vectors and their applications. Blackwell Scientific Publishers. phosphoinothricin. chemical mediated transformation. Trease & Evans. Chichister.1999. glyphosate. Pearson Education limited. BT344 ADVANCED PLANT BIOTECHNOLOGY Credit : 4:0:0 UNIT-I: Genetic engineering: Introduction to Gene Manipulation. thionins. gene knock out and silencing and RNAi. R. Hughes. chitinase. coat protein mediated.Chand & Campany Ltd. J. Wiley. ACC oxidase. Preparation of probes. E. A. electroporation. A Text Book of Biotechnology.S. UNIT-II: Methods in genetic: enzymes as molecular tools . Agarose Gel Electrophoresis.1985. DNA modifying enzymes and their applications in recombinant DNA technology. References 1. Particle bombardment.Pvt. molecular probes. Harcourt Brace & Company.F. S. Basic Techniques: Isolation and purification of Nucleic Acids (DNA.A. nematode resistance. nucleotide labling. Mantel S. Nucleic acid microarray.School of Biotechnology and Health Sciences Department of Biotechnology (BT) 2. Southern. silicon carbide mediated and floral dip method. Sambrook. Mathews 5.

Production of recombinant proteins Outcome At the end of the course. Process flow sheeting – block diagrams. (2003) Reference Books: Karunya University  Page 627  . amylases. cellulases and Enzyme Inhibitors: inhibitors of cholesterol synthesis. butanol. Glick and Pasternak. production of some commercially important modern Bioproducts. Products of plant and animal cell cultures. 2. Biofertilisers. 2001. Unit – II Production of Primary Metabolites (13) A brief outline of processes for the production of some commercially important Organic acids: citric acid. Wulf Cruger and Anneliese Crueger. 09BT201 BASIC INDUSTRIAL BIOTECHNOLOGY Credit : 4:0:0 Objective To impart the knowledge on Historical overview of Biotechnology. Products of plant and animal cell cultures. lipases. Vitamins: Vit. Industrial Microbiology. Vit. A brief survey of organisms. New Age International (P) Ltd. Biotechnology: A Textbook of Industrial Microbiology.A and Steroids..Betalactams: penicillin. Industrial Enzymes. L. Aminoglycosides: streptomycin. acetic acid. the students would have learnt about the steps involved in the production of bioproducts and methods to improve modern biotechnology Unit – I Introduction to Industrial Bioprocess (13) A historical overview of industrial fermentation process – traditional and modern biotechnology. Biopolymers: xanthan gum. Molecular Biotechnology. products relating to modern biotechnology. Total : 65 hours Text Book: 1. B2.B12. Unit – V Production of Modern Biotechnology Products (13) Production of recombinant proteins having therapeutic and diagnostic applications: vaccines. Panima Publishing Corporation. PHB and Single cell protein. Production of Biopesticides. Unit – IV Production of Enzymes and Other Bioproducts (13) Production of Industrial Enzymes: proteases.School of Biotechnology and Health Sciences Department of Biotechnology (BT) 7. Unit – III Production of Secondary Metabolites (13) Study of production processes for various classes of secondary metabolites: Antibiotics. phenyalanine. Amino acids: glutamic acid.E. Biopreservatives: Nisin. Macrolides: erythromycin. monoclonal antibodies. Cheese. Casida Jr. lactic acid. cephalosporin. (2000). pictorial representation. lysine. processes. aspartic acid and Alcohols: ethanol.

IInd edition. Murrey Moo & Young. preparations. types . Dunn. Flame Photometer and Spectrophotometer (13) Beer . adsorption. supporting materials-paper.2006 2. Unit II Colorimeter. GM counter. determination of pH using H2 electrode and glass electrode. Cambridge University press. gas and HPLC. Scintillation counter.Principle and Types. Industrial Microbiology. immuno-electrophoresis.principles. thin layer. the students would have learnt about principles of spectroscopy.. Electrophoresis – principles. Outcome At the end of this course.Lambert’s law. Pergamon. gel filtration. Autoradiography. radioactive decay and their types.thermo gravimetric. Comprehensive Biotechnology. Ratledge & Kristiansen.paper. 2004. types – disc. radioactive techniques-RIA. Basic Biotechnology. starch. description and application of Colorimeter.School of Biotechnology and Health Sciences Department of Biotechnology (BT) 1. derivation of Henderson-Hasselbalch equation and its application. Centrifugation. ion-exchange. 09BT202 INSTRUMENTAL METHODS OF ANALYSIS Credit: 4:0:0 Objective To develop skills of students in instrumentation and biological techniques. affinity. polyacrylamide. buffering systems of blood. Unit V Thermo Analytical Techniques (8) Theory of thermal analysis. Agrobios (India). nephelometry & chromatography and all biotechniques Unit I Centrifugation and pH Measurement: (13) Definitions. agarose. Flame photometer and Spectrophotometer: types– UV – visible – IR – Raman spectroscopy. Presscott. Applications in Medicine & Diagnosis. Unit IV Radio active techniques (13) Radioactive isotopes. Principle. Unit III Chromatography and Electrophoresis (18) Chromatography. Flourimeter.Basic theory.2007 3. construction and working of Differential Thermal Analysis (DTA) and Differential Scanning Calorimeter (DSC) Karunya University  Page 628  . calibration of instrumental methods. Isoelectric focussing. Flourimeter.

. fluid static’s. Morality. H.Atomic. weight fraction. Karunya University  Page 629  .continuity And mechanical energy equations.moles.Velocity potential . 2002. Reference Books: 1. Material Balance with and without chemical reaction (13) Laws of conservation of mass. McGraw Hill. Heads losses for various stations. crystallization. specific gravity. problems. molecular & equivalent weights. Instrumental Methods of Analysis. CBS Publishers & Distributors.density. bypass and purging. mixtures and solutions.pressure measurementsDimensionless analysis and similitude. Skoog.Types of fluids. McGraw Hill Book Company. 1989. drying etc Material balance with Chemical reactions.meaning of material balance and its applications-like distillation . 2. recycle .School of Biotechnology and Health Sciences Department of Biotechnology (BT) Total: 65 Hours Text Book: 1. Ideal gas law-Ideal mixtures and solution-Dalton aw of additive volumes. Concept of Simpson rules and its application Unit – II. limiting and excess reactant. Outcome To familiarize • Units and Basic calculation • Conservation and application • Study of fluids • Mechanism of Fluidization • Transportation of fluids Unit-I Importance of units and Basic Calculation (13) Conversion factors. mole fraction. Willard and Merrit. Instrumental methods of Chemical Analysis. Fluid mechanics (13) Nature of fluids .evaporation.Molar concept. modality and normality. velocity profile and friction factor for smooth and rough surface pipes. Instrumental Methods and Analysis. VI Edition.III. 2000. Introduction to Chemical Analysis. 1987 09BT203 PRINCIPLES OF CHEMICAL ENGINEERING Credits: 4:0:0 Objective To develop skills of students in principles and basic calculations of chemical Engineering. Conservation of energy. Braun.Meaning of energy balance and its applications Unit. 2.properties of fluids. D. Ewing GW.

Venkataramani and N. Sixth Edition.. 2000. 5... Screening Analysis 09BT205 UNIT OPERATIONS Credits: 4:0:0 Objective This course aims at making the students understand the fundamental principles and concepts of heat transfer and mechanical operation in biochemical processes. 2004. Pressure drop in packed bed column 6.. “Elementary Principles of Chemical Processes”. Head loses in pipe fitting 4. Prentice-Hall of India Pvt.. globe and ball valve. Fourth Edition. Fluid flow measurement (13) Measurements of fluid flow – orifice meter. 2. Selection and specification .volume -1. “Basic Principles and Calculations in Chemical Engineering”. types. V. and Rousseau R. reciprocating pumps. John Wiley and Sons. Air lift and diaphragm pumps positive displacement pumps. Total : 65 hours Text Books: 1. Third Edition. centrifugal pumps .M.mechanism . Pressure drop in fluidized bed column 5. Ltd.. its application. Process Calculations. 2003 09BT204 CHEMICAL ENGINEERING LAB Credit: 0:0:2 List of Experiments: 1.V Transportation of Fluid (13) Transportation of fluids – fluids moving machinery performance.Chemical process calculation –by Ghavene – Delhi publications. Unit. venturimeter.Unit operation of Chemical Engg “ Chattopathya” Khanna publication. Flow controls .Harriot. Flow measurement through venture meter 3. needle valve . 2004. pump characteristics. Over all heat transfer coefficient in double pipe heat exchanger 10.Tata McGraw hill – 2001.BI Bhatt &SM vora “ Stoichiometry” Tata Mcgraw. 2. wires and notechs.2007 Reference Books: 1.Anantharaman. butterfly valve.School of Biotechnology and Health Sciences Department of Biotechnology (BT) UNIT –IV. Inc. Concepts of compressors .Unit Operation of Chemical Engineering by McCabe and Smith. Flow measurement through orifice meter 2. Fluidization. Over all heat transfer coefficient in shell& tube heat exchanger 9. Friction factor for packed beds. Flow through helical coil of different diameter pipe 7.Hill. fans and blowers. Himmelblau D.gate valve ..2002 3.M. Ergun equations. Felder R.W. Over all heat transfer coefficient in plate heat exchanger 8. Karunya University  Page 630  . 4. pitot tube. Rota meter.

Fouling factor . mean temperatures.McCabe W. Emulsifying – rpm. Unit – V. Fourier’s law of heat conduction. individual and overall heat transfer coefficients. Heat conduction thro the composite wall .Geankoplis C.Types of evaporators. gas-liquid systems. application of LMTD. Types of feeding. Steam economy. Filter aids – Filter theory .C.L. Sedimentation. Unit-IV. Karunya University  Page 631  .Mcgrawhill.J. agitation of liquids. Unit Operations In Chemical Engineering.steady state – unsteady state heat conduction. Thermal conductivity.5th Edition. Unit-III. Prentice Hall India. Batch sedimentation. design of heat exchanger. Concept of heat conduction.2002.. Smith J.1993. Agitator scale up. selection of medium. free settling and Hindered settling. gas-solid suspensions. Particle Size. Grey and Block bodies. Filter media . temperature. Mixing and Agitation (13) Dimensional analysis. Reduction. Visual Density.Types of filtration . Heat transfer in packed and fluidized beds. Heat Exchanger (13) Heat exchanger – Types of flow. Centrifugal and centrifuge. Total : 65 hours Text Books: 1. Stability. Unit – II. Convection and Radiations (13) Concept of heat transfer by convection. mixing of Powder. 2. Concept of Radiation. Evaporator. Heat transfer to boiling liquids. Industrial filtrations. constant volume filtration.School of Biotechnology and Health Sciences Department of Biotechnology (BT) Outcome To expose the students of biotechnology to • Principles and mechanism of heat transfer • Fundamentals of convectional heat transfer • Basic principles of heat exchanger design • Concepts of drying and thermal processing of biological materials Unit–I Heat Transfer. Gear motor system. Types of convection. Transport Processes And Unit Operations. Heat transfer from condensing Vapours. Types of heat exchanger . Mechanical separation (13) Filtration .how to prevent the dirt factor. hallo spheres and hallo cylinder. modes of heat transfer. power for agitation. Stabilizers. Transition and Turbulent conditions. Viscous material and pastes.parallel and counter flow heat exchanger .Laws of radiations. LMTD. Types of filter – Constant pressure filtration. Basic considerations and conduction (13) Importance of heat transfer in chemical and biotech operations . application of dimensional analysis for convection: Derive the equation for laminar . Calculations of Material and Energy balances .concept of Evaporator capacity.

P. Principles of Ion –Exchange. Unit – V Leaching and Extraction (13) Solid – liquid equilibrium. Theory of mass transfer . Total : 65 hours Karunya University  Page 632  . Fundamentals Of Heat And Mass Transfer. Desgn of absorption towers . Design calculations of by McCabe –Thiele method and Ponchon Savarite methods. Leaching equipment. overall mass transfer coefficient . John Wiley. Equlibrium in ternary systems . different contact Extractors. Azeotropic distillation. molecular distillation. Industrial equipments .School of Biotechnology and Health Sciences Department of Biotechnology (BT) Reference Books: 1. Ordinary diffusion in multicomponents gaseous mixtures. Fractional distillation.Batch and continuous types: Calculations of number of stages. Types of distillation – Simple distillation . Unit –III Absorption (13) Theories of gas absorption. Unit – IV Adsorption Ion-Exchange (13) Theories of adsorption of gases and liquids : industrial adsorbents. Types of packing and Characteristics . Various industrial distillation – Extractive distillation . absorption with chemical reaction. Concept of mass transfer coefficient. diffusion in solids . Raoults law derivations from ideality . Adsorption equipments for batch and continuous operation.liquid equilibrium • To study in detail the principles of adsorption • To introduce the concept of membrane separation Outcome At the end of the course the students would have learnt about the basics of the mass transfer process in biotechnological process. Interface mass transfer . methods of distillation – Batch and continuous distillation. steam distillation. flash distillation. Steady state diffusion under stagnant and laminar flow condition. Analgies between momentum and mass transfer coefficients. Concept of NTU and HTU. batch and continuous extractors. Unit-I Diffusion in fluids (13) Molecular and Eddy diffusion in a gas and liquid . Unit-II Distillation (13) Vapour – Liquid equilibrium diagram.1998 09BT206 MASS TRANSFER OPERATIONS Credits: 4:0:0 Objective • To introduce the mass transfer principles • To study the vapor liquid equilibrium • To teach the concept s liquid . Diffusion measurement and calculations. Incropera F.

Mass transfer –II K. Bioenergetics: redox biochemistry. Lys. sphingolipids. conjugated proteins.Mc Cabe & Smith. 7th edn. modified amino acids.gavhane. International edition.I: Structure and Properties of Carbohydrates and Lipids (13) Structure and properties of mono. phospholipids.badger and banchero. peptides.R. di. composition and function of various biomolecules Outcome At the end of the semester the students will enable to understand the metabolism of carbohydrates. respiratory chain. nucleosides. nucleoprotein complexes.W. Phe.Unit operations of chemical engg. Trp. lipids. Unit-V: Metabolism Of Proteins And Nucleic Acids (13) Biodegradation of proteins. Unit . 2001 3. “ Mass Transfer Operations” McGraw Hill-2003 2. complex carbohydratesStructural and functional relationships.2007 Reference books: 1. Biosynthesis and biodegradation of purines and pyrimidines. 7th edn. Structure and properties of purines. 2005 09BT207 BIOCHEMISTRY Credit: 4:0:0 Objective To study about the structure.Leu.2003 5. Tyr. Tata McGraw Hill. oligo and polysaccharides. Biosynthesis and biodegradation of important amino acids. Tata McGraw Hill. Inborn errors of carbohydrate metabolism. proteins. Glutamate and Cys Transamination and Urea Cycle. Unit – II : Structure and Properties of Proteins and Nucleic Acids (13) Structure and properties of amino acids. energy rich compounds.Unit operation of Chemical Engineering – Chattophathya – Khanna publication-2005 2.A. Unit – III : Intermediary Metabolism And Bioenergetics (13) Overview of intermediary metabolism: Interconnections of metabolic pathways. Total: 65 Hours Karunya University  Page 633  . Inborn errors of their metabolism. pyrimidines. Structure and properties of fatty acids.E Treybal. Unit – IV : Metabolism Of Carbohydrates and Lipids (13) Lipid Metabolism: Biosynthesis and biodegradation of fatty acids and cholesterol.ribonucleic acids and deoxy ribonucleic acids.L McCabe Smith and Harriot P “ Unit Operations of Chemical Engineering” Sixth edition McGraw Hill .coulsion Richardson. Carbohydrate Metabolism: Glycolysis. Thr. nucleotides. glycogenesis and glycogenolysis. 2005 4.School of Biotechnology and Health Sciences Department of Biotechnology (BT) Text books: 1.vol-2. glycolipids and cholesterol. proteins and the bioenergetics.Introduction to chemical engineering.Chemical engineering. polynucleotides .Nirali publications. TCA cycle. pentose phosphate pathway. gluconeogenesis. Met. oxidative phosphorylation and triose phosphate cycle.

Mechanism of SN1 and SN2 reactions. Lubert Stryer. 2007. Estimation of Amino Acid By Ninhydrin Method 7.. Extraction of Total Lipids B. Unit – II Chemistry of enzymes (13) Karunya University  Page 634  .Qualitative Analysis of Carbohydrates 3. A. Enantiomers. Rodwell. M. New York. Study of Biochemical Units And Measurements 2. B. Principles of Biochemistry Third Edition (Freeman Publishers).K. 3. Qualitative Analysis of Amino Acids 5. 2000. L.R. and Cox. proteins and kinetics of various biochemical reactions Outcome At the end of the semester the students will understand various biochemical synthesis. 2nd Edition. Estimation of Cholesterol By Zak’s Method 10.... P. M. 2. Estimation of Ascorbic Acid By Titration Method 11.K. . A. Saponification Characteristic of Lipids 12. Estimation of Protein By Lowry’s Method 6. E. Voet and Voet. WH Freeman & Co. Lehninger. 5th edition 2008 Reference Books: 1. (2000). V. Z Isomerism. L.W. D. Harper’s Biochemistry.. S notation. Biochemistry. Murray. Mechanisms of E1 and E2 reactions.Estimation of Glucose by Glucose Oxidase Method 4. optical activity and optical rotation-polarimeter. 4th Edition. Granner. Dry Ashing of Food Materials And Colorimetric estimation of Phosphorus 09BT209 BIOORGANIC CHEMISTRY Credit: 4:0:0 Objective To impart knowledge about biocatalyst.. Nelson.3rd edition 2008. protein kinetics and the mechanism involved in biochemical reactions Unit – I Concepts in organic chemistry (13) Stereochemistry. R. Estimation of DNA By Burton’s Colorimetric Method 9. Biochemistry. Prentice Hall International.. Assay of Acid Phosphatase enzyme in Potato Extract 8. 27th edition. Optical isomers.School of Biotechnology and Health Sciences Department of Biotechnology (BT) Text book: 1. Mayes. John Wiley & Sons Inc. 09BT208 ANALYTICAL BIOCHEMISTRY LAB Credit: 0:0:2 1..A.

Organic Chemistry. Jain & Sunjay Jain. Text. molecules that control cell cycle. 2.an overview. Concept of Free energy and its importance in biology. 09BT210 CELL BIOLOGY Credit: 4:0:0 Objective: To know the morphology and functional aspects of cell Outcome: The students will be able to understand type of cell. Finar.School of Biotechnology and Health Sciences Department of Biotechnology (BT) Structure of atom.H. symport. New Delhi. Carboxy peptidase. its morphology. Stereochemistry of amino acids. antiport. Unit – III Case studies of enzyme structure and mechanism (13) Acid base and covalent catalysis. Application of enzymes in industry and medicine. Total: 65 Hours Text books: 1. Ca2+ ATPase pump.L. New Delhi. Biochemistry by Mathew . Organic chemistry by Paula yurkaris Bruice. components and applications in Biology. extra cellular matrix. permeases. cytoskeletal proteins. cell culture and transport of various nutrients across the cell wall Unit – I Structure and Function of the Cell and Its Organelles (13) Eukaryotic and prokaryotic cells: principles of membrane organization . lysozyme. 6th Edition. Cell division: mitosis & meiosis. Structure and mechanism of pyruvate dehydrogenase. cell cycle. 2000. Text book of Bio-organic Chemistry. New Delhi. Chemistry of active oxygen species. 5th edition. lysosomal and vacuolar membrane ATP dependent proton pumps. transKarunya University  Page 635  . signaling. Basic methods of protein folding – two state kineticsmultistate kinetics-in protein folding. stability of proteins and molecular chaperones. 5. sodium potassium pump. Bioorganic chemistry. Unit IV: Hydrolysis in chemical synthesis (13) Ester hydrolysis. types. Pearson P Ltd. Trevor Palmer. 3. Amide hydrolysis. Reference Books: 1. 2002. Springer Verlay Publishers.Principles. Vol:2. 2007. peptide synthesis. UNIT V : Protein folding kinetics and folding pathways (13) Structure of proteins. NAD. proteases. I. 2005. East West Press P Ltd. Van Holde.Micelles. 2004. 4. its dynamics. Unit – II Transport Across Cell Membranes (13) Passive & active transport. structure and mechanism of FAD THF coenzymes. Chemical bonding involved in protein structure. . ribonucleases. 3rd edition. contractile proteins – actin & myosin. H. Dependent oxidation and reduction reactions. 2002. Fundamental of Biochemistry by J. . Athern . membrane proteins. Protein folding. co transport. Enzymes. chemical bonding and its characteristics. Dugas.Dugas . free radicals systems. NMR. L. Pearson Publishers Ltd. Circular dichorism . Pearson Education. Specificity of enzymes. 1999. 2. Springer Verlag Publishers.

Geoffrey M. role of matrix in cell growth. phosphorylation and regulation of protein kinases in signalling. serine – threonine kinases in signalling. autocrine. The paper also throws light on multifarious habitats of microbes. Inc. immunochemistry. biosynthesis of inositol tri phosphates and their role as messengers. De Robertis & De Robertis. role of cyclic AMP. examples of receptors. Kimball T. Unit – I Introduction (13) Basic of microbial existence. contamination. primary cultures. Outcome To impart knowledge on classification of microbes and to improve the knowledge on genomic structure of microbes and applications of genetic engineering. different models of signal amplifications. principles of different staining techniques like gram staining. Cell Biology.Watson. cyclic GMP and G proteins in signal transduction. Total:65 Hours Text Books: 1.W. 2005 09BT211 MICROBIOLOGY Credit: 4:0:0 Objective This paper enables the students to identify any microorganisms and its applications. explant cultures. ion-selective gated channel against neuronal cell membrane. This paper deals with various types of classification of microbes. characterization of cells. techniques in cell culture. New York.School of Biotechnology and Health Sciences Department of Biotechnology (BT) membrane potential coupled ATP generation.2007 2. Cooper and Robert E. capsular staining. 4nd edition. calcium ion flux and its role in cell signaling. maintenance of stock cells. Transport into prokaryotic cells. Alan R.. ASM Press and Sinauer Associates. Culture of Animal Cells. endocytosis and exocytosis. microscopic examination of microorganisms.. three dimensional cultures. Hausman The Cell: A Molecular Approach. history of microbiology. Unit – IV Signal Transduction (13) Signal amplification. Entry of virus and toxins into cells. Fourth Edition. quantitation and characterisation of receptors.. paracrine and endocrine models of action. Wesley Publishers. morphological analysis. Unit – V Cell Culture (13) Techniques for the propagation of eukaryotic and prokaryotic cells. 2006 3. 3rd Edition. 2007 2. Ian Freshney R. classification and nomenclature of microorganism. 4th Edition. Unit – III Receptors and Models of Extra Cellular Signalling (13) Cytosolic. Unit – II Microbes-Structure and Multiplication Karunya University  (13) Page 636  .USA. light and electron microscopy. 2005 Reference Books: 1. nuclear and membrane bound receptors. Cell line: generation of cell lines. Cell Biology. Molecular Biology of the Cell. flagellar staining. James D. acid fast. differentiation. Liss. Inc.

Nepal. Casita. 2001. 2003. Growth And Metabolism (13) Nutritional requirements of bacteria and different media used for bacterial culture. Foundations in Microbiology. clinically important microorganisms.N. Spore stainig 8. Phenol coefficient test 10. yeast. microorganisms and pollution control. anti-bacterial. Wm.. G.. 2001.N. Prescott LM. Klein DA. Reference Books: 1. secondary metabolites and their applications. biosensors. Sterilization and Disinfection 3. India.O. New Delhi. Microbiology. leaching of ores by microorganisms. Motility test. alcohol.B12.Brown Publishers. Oxford & IBH. mycorrhizae (AMFungi) and biopesticides. Box 20195. Pelczar MJ. mode of action and resistance to antibiotics. Kathmandu. utilization of energy for biosynthesis of important molecules. aerobic and anaerobic bioenergetics. Microscopy 2. Vit. 3rd Edition. 3.Hanging drop method and soft agar inoculation Karunya University  Page 637  . Unit – V Industrial and Environmental Microbiology (13) Primary metabolites. Unit – IV Control of Microorganisms (13) Physical and chemical control of microorganisms. viruses. Prasad B. Gram staining 7. bioremediation.C. cyanobacteria. C. Inoculation of microorganisms 5. production of penicillin. Unit – III Microbial Nutrition. Pelczar and Reid. Total:65 Hours Text Book 1. W. Isolation of pure culture by streak plate technique 6. Chan ECS And Krein NR. biogas. Harley JP. antifungal and anti-viral agents.P. “A Text Book of Biotechnology”.. 3. Tata McGraw Hill Edition. 2. 2003. Microbiology. host-microbe interactions. algae and fungi with a special mention of life history of actinomycetes. Talaron K. preservation of food. New Delhi. “Biotechnology in Sustainable Biodiversity and Food Security”. Culture Media – Types & Preparation of Agar medium and Nutrient Broth 4. 09BT212 MICROBIOLOGY LAB Credit: 0:0:2 List of Experiments 1.2007 2. mycoplasma and bacteriophage. Talaron A. Budha Academic Enterprises. Negative stainig 9.School of Biotechnology and Health Sciences Department of Biotechnology (BT) Structural organization and multiplication of bacteria. Prasad B. Brown Publishers. growth curve and different methods to quantitate bacterial growth. biofertilizers rhizobia.

UNIT – I Classical Genetics (13) Mendelian genetics. Total: 65 Hours Text Books 1. inhibitors. Benjamin Lewin. 2008 Karunya University  Page 638  . transcription and translation processes in prokaryotes and eukaryotes.RNA splicing. UNIT – V Regulation of Gene Expression (13) Regulation at various stages of gene expression in eukaryotes and prokaryotes . post-transcriptional modification . Eighth Edition.David Friefelder. DNA repair. features of promoters and enhancers. Oxford University Press.Lac and trp operons. mutation. transcription factors. classical experiments – Hershey and chase. process of translation in prokaryotes and eukaryotes. post-translational modifications. Molecular Biology. UNIT – II DNA Replication (13) Replication in prokaryotes and eukaryotes. D-loop and rolling circle mode of replication. crossing over. Outcome The major objective of the paper is to provide knowledge of molecular biology and genetics of prokaryotic and eukaryotic organisms to the students. Enumeration of microorganisms from soil 12. RNA editing.2005 Reference books 1. suppressor mutation.School of Biotechnology and Health Sciences Department of Biotechnology (BT) 11. replication of linear viral DNA. Gene IX. Bacterial conjugation. John Wiley. Narosa Publ. various mutations. Gardner / Simmons / Snustad. This paper provides insight on replication. Principles of Genetics. House. Organization of eukaryotic chromosome – cot value. ribozyme. linkage. repair in eukaryotes and prokaryotes and in cancer. 1999 2. Enumeration of microorganisms from water 09BT213 MOLECULAR BIOLOGY Credit : 4:0:0 Objective Helps the student to understand and apply this knowledge in research to study the molecular mechanism of DNA and RNA synthesis and Protein synthesis. inhibitors of protein synthesis. transduction and transformation. UNIT – III Transcription (13) Prokaryotic and eukaryotic transcription. their repair mechanisms and cancer genetics. UNIT – IV Translation (13) Elucidation of genetic code. replication of telomeres in eukaryotes. Avery McLeod & McCarty.

gene-therapy. UNIT – V Applications of Recombinant DNA Technology (13) Cloning in plants. Molecular Biology of the Gene. safety guidelines of recombinant DNA research. Moore DD. genetic elements that control gene expression. 2. genomic library. PCR and other applications of genetic engineering UNIT – I Basics of Recombinant DNA Technology (13) Role of genes within cells. Academic Press. Yeast and Mammalian vectors. "Principles Of Gene Manipulation. Recombinant cytokines and antibodies. embryo transfer technology. An Introduction To Genetic Engineering ". 1993. Hopkins WH. Brent R. Diagnostic importance. Weiner AM. Karunya University  Page 639  .. (Kunkel’s Method). Watson JD. Kimmer AR. UNIT – IV Polymerase Chain Reaction (13) Inverse PCR. 1-3. Berger Sl. stem cell therapy. “Molecular Cloning” 1. UNIT – III Construction of Libraries (13) Construction of cDNA and genomic libraries. Insect.School of Biotechnology and Health Sciences Department of Biotechnology (BT) 2. Kingston RE. "Current Protocols In Molecular Biology ". design of linkers and adaptors. Roberts JW. Invitro fertilization.Sanger’s method. vaccines. NY. Steitz JA. RACE PCR. Nested PCR. Reference book: 1. r-DNA technology. UNIT – II Creation of Recombinant Molecules (13) Restriction mapping. Screening of libraries with DNA probes and with antisera. 1987 3. Greene Publishing Associates. "Methods In Enzymology". Site Directed Mutagenesis. RAPD. mutation. restriction and modifying enzymes. Molecular beacons. Vol 152. prokaryotic and eukaryotic expression vectors. 1988. 1987 06BT214 GENETIC ENGINEERING Credit : 4:0:0 Objective Helps the student to understand and apply this knowledge in research to study the molecular mechanism of DNA and RNA synthesis and Protein synthesis. Total : 65 hours Text Book: Old RW. Primrose SB. Blackwell Science Publications. Taqman assay. Sambrook et al Vol. methods of nucleic acid sequencing. Ansubel FM. Characteristics of plasmid and phage vectors. Outcome At the end of the semester the students would have learnt about r-DNA technology. transgenic and knockout animals.

Unit I: Outlines of Immunology (13) Types of Immunity . Immunodeficiency diseases. also familiarize themselves with immunopathology and immunotherapy. B Cells. Karunya University  Page 640  . DNA Ligation 9. immunodiagonosis. Restriction enzyme digestion 8. Isolation of genomic DNA from animal tissue 4. Immunoglobulin Genes. NK Cells. antigen antibody response. Antibodies and Complements (13) Antigens. Molecular weight analysis using Agarose Gel electrophoresis 10. Autoimmunity. immunopathology etc.Primary and Secondary . Antigen Antibody Interaction. Suppressor T cells and Regulatory T cells. Cytokines and Cytokines. Transformation of cells 11.Humoral and Cell mediated Immune Response. Subtypes of T Cells and their functions: Cytotoxic T cells.Types. MHC and its significance. Tumour ImmunologyTumour Associated antigens and Tumour Specific Antigens. Antigenicity and Immunogenicity. Students.Macrophages. Agarose Gel Electrophoresis-genomic DNA 6. Mast cells. Isolation of genomic DNA from plant tissue 2. mechanisms and disorders. Helper T cells. Transplantation Immunology. function and biological properties of different classes of Immunoglobulins. Isolation of plasmid DNA from E. Immunoglobulins – Structure.their biological role. Phagocytosis. Lymphoid Organs.Innate and acquired immunity. Isolation of DNA and spectrophotometric analysis 5.School of Biotechnology and Health Sciences Department of Biotechnology (BT) 09BT215 MOLECULAR BIOLOGY AND GENETIC ENGINEERING LAB Credit: 0:0:2 List of Experiments: 1. Unit II: Immue Response (13) Cells of Immune system.coli 7. Unit III: Antigens. SDS-PAGE electrophoresis. Unit IV: Immunopathology and Immunologic disorders (15) Hypersensitivity: Types. Isolation of genomic DNA from Micro-organisms 3. Competent Cell preparation 12. T cells. 09BT216 IMMUNOLOGY AND IMMUNOTECHNOLOGY Credits: 4:0:0 Objective This course aims to develop the skills of the students in Immunotechnology. Outcome At the end of the course would have learnt about the mechanisms by which a human body interacts with a pathogenic microbe & how it eliminates it. Complement activation – Classical and alternative pathways. Immunotherapy.

Keith Roberts and James D Watson (Eds.Essential Immunology. Cellular and Molecular Immunolgy. Brudce Alberts. Dennis Bray. New York. W.. 2000. Lichtman & Jordan S. J. Freeman and Company. Monoclonal and Polyclonal Antibody Production. Immunology. Immunofluorescence. J. W. Immunocytochemistry. virus. Jonathan Brostoff. 2002. 10th Edn. Martin Raff. Churchill Livingston. 2 3 4 Weir DM and Stewart. Immunology. ELISA and Elispot Assay. Text Book: 1 Kuby. 3rd Edn. Immunolgy.H.. Immunological Techniques – RIA.. Blackwell Scientific Publication. 5th Edn. . Andrew K. 2000. Pober (Eds. Abbas. Julian Lewis. fungi and parasites. 10th Edn. Saunders Company. New York. David Male (Eds. Mosby Publication. Active Immunization and Passive Immunization. 2002 2 Abul K.). Vaccines. Ivan Roitt.).) Molecular Biology of the Cell (5th Edn).B.School of Biotechnology and Health Sciences Department of Biotechnology (BT) Unit V: Immunity to Infections and Immunotechnology (15) Immunity to bacteria.. 2001 Reference Books: 1 Ivan Roitt. 09BT217 CELL BIOLOGY LAB AND IMMUNOLOGY LAB Credit: 0:0:2 List of Experiments: 1 Study of Microscopy 2 Microscopically Identification of Cells in Permanent Fixed Slides 3 Staining for Various Stages of Mitosis in Allium cepa (Onion) 4 Osmosis and Tonicity Studies Using Red Blood Corpuscles 5 Differentiation of Blood Cells Using Giemsa Staining 6 Separation of Peripheral Blood Mononuclear Cells and Trypan Blue Assay for Live Cell 7 Blood Grouping and Rh typing 8 Preparation of Plasma and Serum 9 Single Radial Immunodiffusion 10 Double Immunodiffusion – Ouchterlony Method 11 Immunoelectrophoresis 12 Counter Current Immunoelectrophoresis 13 Rocket Immunoelectrophoresis – Laurell method 14 Immunodiagnosis of Typhoid fever – Widal Test 15 Enzyme Linked Immunosorbent Assay Karunya University  Page 641  . 2002.

design of various commercial media for industrial fermentations – medium optimization methods UNIT – III Sterilization Kinetics (13) Thermal death kinetics of microorganisms. Karunya University  Page 642  . oxygen requirements. general requirements of fermentation processes. Metabolic stoichiometry. air sterilization and design of depth filters. medium formulation of optimal growth and product formation. UNIT – IV Metabolic Stoichiometry and Energetics (13) Stoichiometry of cell growth and product formation. carbon.School of Biotechnology and Health Sciences Department of Biotechnology (BT) 09BT218 BIOPROCESS PRINCIPLES Credit : 4:0:0 Objective To develop skills of the students in the area of Bio process Technology with emphasis on Bioprocess principles. Kinetics of microbial growth etc. maintenance coefficients energetic analysis of microbial growth and product formation. examples of simple and complex media. yield coefficients of biomass and product formation. elemental balances. minerals. UNIT – I Overview of Fermentation Processes (13) Overview of fermentation industry. This will serve as an effective course to understand certain specialized electives in Bioprocess related fields. batch and continuous heat sterilization of liquid media. the students would have learnt about fermentation processes. Energetics. This is a pre-requisite for courses on Bioprocess Engineering offered in the subsequent semesters. vitamins and other complex nutrients. nitrogen.batch and continuous. medium requirements for fermentation processes. design of sterilization equipment . degrees of reduction of substrate and biomass. main parameters to be monitored and controlled in fermentation processes. Outcome At the end of the course. oxygen consumption and heat evolution in aerobic cultures. basic configuration of fermentor and ancillaries. filter sterilization of liquid media. UNIT – II Raw Materials and Media Design for Fermentation Process (13) Criteria for good medium. available electron balances.

Biochemical Engineering. Principles of Fermentation Technology. 2002 2 Peter F. Production of Wine 12. and Kargi. Culturing of Different Types of Microorganism 2. Total : 65 hours Text books: Shuler. extraction and purification of enzymes and biosensors.batch. Blanch. Enzyme Assay.School of Biotechnology and Health Sciences Department of Biotechnology (BT) UNIT – V Kinetics of Microbial Growth and Product Formation (13) Modes of operation .2007. Components of Fermentor 8. Effect of Substrate Concentration on Growth Of E-coli 4. Ltd. Harvey W. Douglas S.Starch Plate Assay 10. growth of filamentous organisms. product formation kinetics .leudeking-piret models. Ltd. 1986. Simple unstructured kinetic models for microbial growth. Production of Amylase From Bacillus subtilis and Assaying for its Activity 09BT220 ENZYME ENGINEERING AND TECHNOLOGY Credits: 4:0:0 Objective To develop skills of the students in the area of Enzyme Engineering with emphasis on Bioreactor operation and design.2005. Effect of Temperature on Enzyme Activity 6. Immobilization of ∞ . Stephen J. Effect of pH on Enzyme Activity 5. M.. immobilization. Stanbury. the students would have learnt about classification of enzymes.. 1999. Quantitative Enzyme Assay 11. substrate and product inhibition on cell growth and product formation.Basic concepts – Second Edition Prentice Hall of India Pvt. Karunya University  Page 643  . Hall & A. Inc. Pauline Doran. Clark. Determination of Volumetric Mass Transfer Coefficient 9.Amylase Enzyme 7. " Bioprocess Engineering . 09BT219 BIOPROCESS LAB 1 Credit : 0:0:2 Title of the Experiments: 1. Whitaker. Butterworth – Heinemann An Imprint of Elsevier India Pvt.L. Second Edition. Reference Books: 1 2 3 Bailey and Ollis. Monod model.). McGraw Hill (2nd Ed. Estimation of Biomass Production 3.F. Bioprocess Engineering Calculation. Blackwell Scientific Publications. Marcel Dekker. Outcome At the end of the course. fed batch and continuous cultivation. " Biochemical Engineering Fundamentals".

development of enzymatic assays. kinetics of single substrate reactions. Jens Høiriis Nielsen. pharmaceutical and other industries. Contributor Jens Høiriis Nielsen. turnover number. 2001 2. Intracellular and Extracellular enzymes. advantages and disadvantages of different immobilization techniques. encapsulation. animal and microbial sources. Types of enzymes-Constitutive enzyme. specificity of enzyme action . estimation of Michelis – Menten parameters. 2005 2. multi substrate reactions.examples. Fundamentals of Enzymology by Price and Stevens Oxford University Press. UNIT IV ENZYME IMMOBILIZATION (13) Physical and chemical techniques for enzyme immobilization – adsorption.Enzymes in Industry: Production and Applications. UNIT II MECHANISMS AND KINETICS OF ENZYME ACTION (13) Mechanisms of enzyme action. 2005 3. healthcare and environment. Overview of applications of immobilized enzyme system UNIT – V ENZYME BIOSENSORS (13) Types of Biosensors.Wolfgang Aehle Contributor Wolfgang Aehle.Text book 'Enzyme Technology. types of inhibition & models –substrate. Monod changeux wyman model. product. Biocatalysts and enzyme technology. UNIT III PURIFICATION AND CHARACTERIZATION (13) Production and purification of crude enzyme extracts from plant. cross-linking. Klaus Buchholz.School of Biotechnology and Health Sciences Department of Biotechnology (BT) UNIT I INTRODUCTION AND APPLICATION OF ENZYMES (13) Classification of enzyme.Edition: 2. covalent binding etc.mechanisms and kinetics. Cambridge University Press.Published by Wiley-VCH. Sabine Arnold. matrix entrapment. 2002 Reference Books: 1. Dec 2004 Karunya University  Page 644  . Volker Kasche. induced enzymes. methods of characterization of enzymes. 2004 4. Uwe Theo Bornscheuer .Published by Wiley-VCH. . Martin Chaplin and Christopher Bucke. Allosteric regulation of enzymes.Published by Springer. ph and temperature effect on enzymes & deactivation kinetics.Biotechnology for the Future.. Enzymes by Palmer Horwood Publishing Series. concept of active site and energetics of enzyme substrate complex formation. Application of enzymes in food. Total : 65 hours Text Books: 1. design of enzyme electrodes and their application as biosensors in industry. Enzymes for analytical and diagnostic applications.

single cell models. scale-up. State and parameter estimation techniques for biochemical processes UNIT IV MODERN BIOTECHNOLOGICAL PROCESSES (13) Recombinant cell culture processes. Bioprocess design considerations for plant and animal cell cultures UNIT V MODELLING AND SIMULATION OF BIOPROCESSES (13) Study of structured models for analysis of various bioprocess – compartmental models. Total : 65 hours Karunya University  Page 645  . products and other metabolites. plasmid replication and plasmid stability model. power consumption and impeller tip speed UNIT III MONITORING OF BIOPROCESSES (13) On-line data analysis for measurement of important physico-chemical and biochemical parameters. mass transfer correlations. models of cellular energetics and metabolism.School of Biotechnology and Health Sciences Department of Biotechnology (BT) 09BT221 BIOPROCESS ENGINEERING Credits: 4:0:0 Objective To develop skills of the students in the area of Bio process Engineering with emphasis on Bioreactor operation and design. Bubble column reactor. methods for the determination of mass transfer coefficients. plasmid stability in recombinant cell culture. fed batch. Methods of on-line and off-line biomass estimation. limits to over expression. Modelling of recombinant bacterial cultures.microbial oxygen demands. the students would have learnt about Bioreactor operation and design. guidelines for choosing host-vector systems. microbial calorimetry . Outcome At the end of the course. Dynamic simulation of batch. Flow injection analysis for measurement of substrates. oxygen mass transfer in bioreactors . RTD and stability analysis of bioreactors UNIT II BIOREACTOR SCALE-UP (13) Regime analysis of bioreactor processes. packed bed reactor. steady and transient culture metabolism. Bioreactor strategies for maximizing product formation. modeling and simulation . Fluidized bed-bioreactors. This will serve as an effective course to understand certain specialized electives in Bioprocess related fields UNIT I DESIGN AND ANALYSIS OF BIOREACTORS (13) Design and operation of novel bioreactors-Air-lift loop reactors. Scale up criteria for bioreactors based on oxygen transfer.

2002 2. Outcome At the end of the course. Bjorn K. “Bioprocess Engineering Principles”.”Bioprocess engineering (handcover)”. the students would have learnt about various methods of separation and purification of bioproducts. Nancy Ade’lia and Kim Nelson. Academic Press. enzymatic and chemical methods. bioaffinity and pseudo affinity chromatographic techniques. Elsvier Pergamon Press.1994 09BT222 DOWNSTREAM PROCESSING Credit : 4:0:0 Objective To develop skills of the students in the area of Downstream processing with emphasis on purification of products. ion-exchange.School of Biotechnology and Health Sciences Department of Biotechnology (BT) Text Books: 1. dialysis.1995 2. Arno P.SJ Hall “Principles of fermentation technology”. size exclusion.Whitaker. (13) UNIT – III Isolation of Products (13) Adsorption. adsorption. Cell disruption for product release – mechanical.1999 Reference Books: 1.Second edition.filtration and centrifugation. Fikret Kargi.Biwer. reverse phase. Pauline Doran.Stanbury. 3. Pretreatment and stabilisation of bioproducts UNIT – II Physical Methods of Separation Unit operations for solid-liquid separation . 2007. UNIT – I Downstream Processing (13) Introduction to downstream processing principles characteristics of biomolecules and bioprocesses. Elmar Heinzle. instruments and practice. precipitation of proteins by different methods. membrane separation – ultrafiltration and reverse osmosis. Wiley.Wiley Interscience. Karunya University  Page 646  .Second edition. hydrophobic interaction. UNIT – IV Product Purification (13) Chromatography – principles. P. aqueous two-phase extraction. Prentice Hall. A. “Development of Sustainable Bioprocess: Modelling and Assessment”.Lyderson. liquid-liquid extraction. Michael Shuler.”Bioprocess Engineering Principles”.

Haleem J. Regnier. Ltd. Drying of solids by light source 10. Fred E. B. 2006 2.. Gooding. High-performance liquid chromatography (HPLC) 09BT224 METABOLIC ENGINEERING Credit : 4:0:0 Objectives To develop skills of the students in the area of metabolic engineering to alter the metabolic pathway to enhance the product yield.Published by PHI Learning Pvt. Liquid – liquid extraction 8. Roger G. 2002 2. Plate and frame filter press 9. Adsorption 5. Trevor G.Mishra Neeraj. Fred E.Published by CRC Press. Solid recovery by centrifugation 7. Batch Sedimentation 2. 2002 4. Regnier. Karunya University  Page 647  . Gooding.Published by CRC Press. Batch Distillation 4.Published by Academic Press. Isolation and Purification of Proteins.. A Century of Separation Science.Bioseparation Technology. Contributor Karen M.Published by CRC Press.Rajni Hatti-Kaul.. 2000 3.2008 Reference Books: 1. 2002 09BT223 DOWNSTREAM PROCESSING LAB Credit : 0:0:2 List of Experiments: 1. the students would have learnt about various methods synthesis of primary and secondary metabolites and bioconversion.Day. drying and lyophilization in final product formulation. USA.Satinder Ahuja. Bioseperations: Principles and Techniques. Bo Mattiasson. Flocculation 12. Issaq. Sivasankar. Lyophilization ( Freeze drying of culture) 11. Scott R. Publisher: Oxford University Press.. Cell disruption by Homogenizer 3.Bioseparations Science and Engineering . 2003 5.Karen M..Handbook of Bioseparations. and Harrison. (13) Total : 65 hours Text book 1..HPLC of Biological Macromolecules.School of Biotechnology and Health Sciences Department of Biotechnology (BT) UNIT – V Final Product Formulation and Finishing Operations Crystallization.CRC Press. and Rudge. Protein purification by salting –out method (ammonium sulphate) 6. Outcome At the end of the course.

mutants resistant to repression. catabolite regulation by passing control of secondary metabolism. Regulation of RNA synthesis by amino acid. 2005 Reference Books: 1. Lilly M. co-metabolism. recognising growth cycle peak. conversion of insoluble substances. permeability. Ltd. UNIT – II Synthesis Of Primary Metabolites (13) Alteration of feed back regulation. feedback regulation. specificity. Cooney C. Peter F.I. UNIT . induction. Zubay G.. factors important to bioconversion. Total : 65 hours Text books 1. " Fermentation And Enzyme Technology ". " Biochemistry ". Principles of Fermentation Technology. catabolite repression. mixed or sequential bioconversions.IV Bioconversions (13) Advantages of bioconversions. Hall & A. Karunya University  Page 648  .School of Biotechnology and Health Sciences Department of Biotechnology (BT) UNIT – I Introduction (13) Jacob Monod model for gene expression regulation-Lac operon. 1989. improving fermentation. enzyme induction.. catabolite regulation. avoidance of product inhibition. cumulative feedback regulation. Precursor effects.D. Second Edition... Macmillan Publishers. feedback resistant mutants.. limiting accumulation of end products.. regulation of enzyme synthesis.back repression. active transport. UNIT . Butterworth – Heinemann An Imprint of Elsevier India Pvt. alteration of permeability for metabolites. group transportation.C. trophophase. 1980. permeability control: passive diffusion.glucose effect. gene dosage. feed. Feedback regulation.P.L. John Wiley And Sons. regulation in branched pathways.V Regulation Of Enzyme Production (13) Strain selection. Demain A. mutation. UNIT – III Biosynthesis Of Secondary Metabolites (13) producers of secondary metabolites. concerted feedback regulation.differential regulation by isoenzymes.cAMP deficiency. Stephen J. Humphery A..E. 2. yields.L. idiophase relationship. Dunnil. Stanbury... Wang D. Whitaker.

Clonal Propogation of elite species (Micro Propagation). practical application of somatic hybridization and cybridization. Applications of transgenic plants. biological fluids tissue extract. perfumes. Transgenic plants Technique of transformation – Agrobacterium mediated and physical Methods. Plant cell cultures for the production of useful chemicals : pigments. isolation of tissue. UNIT –I Introduction to Plant Biotechnology (13) In-vitro Methods in plant tissue culture. horticulture and forestry. Outcome At the end of the course. and use of growth regulators. Importance of Serum in media 2. Production of recombinant Vaccines and hormones Interleukines. somatic hybridization.Somaclonal Variation and their significance. In-Vitro production of secondary metabolites – Techniques and significance. Embryo and Endosperm culture and their applications Organogenesis and Somatic Embryogenesis – Techniques and applications. Nutrient media. insecticides. Electroporation and Biolistics(Microprojectile bombardment ) UNIT – IV Animal Cell Culture and Gene Cloning (10) Culture Media for growth of animal cells 1. methods of protoplast fusion –chemical and electro fusion. b) Secondary Culture – transformation and transfection of animal cell lines. Edible Vaccines from plants. Herbicide resistance and stress tolerance in plants. Organ Culture – Anther. Chemically defined media a) Primary Culture – Cell lines. HAT selection. flavors. maintenance of continuous cell lines. and mechanical disaggregation. Bioinsecticdes and Biofertilizers . and cloning disaggregation of tissue.School of Biotechnology and Health Sciences Department of Biotechnology (BT) 09BT225 APPLIED PLANT AND ANIMAL BIOTECHNOLOGY Credit: 4:0:0 Objective To develop skills of the students in the area of plant and animal biotechnology and its applications. the students would have learnt about various Plant and animal tissue culture methods and its applications. Karunya University  Page 649  . regeneration and viability test. Natural media –Plasma Clot. enzyme disaggregation. Aseptic Techniques. Stem cell Culture and applications . UNIT – V Animal Genetic Engineering (10) In vitro fertilization and embryo transfer. anticancer agents and pharmacologically important compounds. UNIT – III Plant Genetic Engineering (13) In-Vitro fertilization – Ovary and Ovule culture. UNIT – II Plant Tissue Culture (19) Methods in Biotechnology Protoplast Culture – Isolation. Selectable Makers etc. Role of tissue culture in agriculture.Techniques for gene transfer in animals.

. Walker and Rapley. 2000 09BT226 BIOETHICS.K.2003 5.theories about right action . Gupta P. Dermal. Plant Biotechnology. Meerut.Gilligan's theory . Cytotoxicity. Pvt. Chand & Co. Panima.. S.C. Eye. 2006 Reference Books: 1. Molecular Biology and Biotechnology. Neurobehaviour. 2006 4.J. Oxford University Press. Unit-III Intellectual Property Rights (13) TRIPS. Reproduction. Elements of Biotechnology. R. Oxford University press. LD50.Self-interest . Bioremediation. Carcinogenicity and Mutagenicity. Text Book of Biotechnology. Mucous. 2005 3. Oxford University press. IPR AND BIOSAFETY Credits: 4:0:0 Objective To impart knowledge on moral issues. Outcome: At the end of the course. 2006 2. Identification of directions for yield effect in agriculture.R.W.Kohlberg's theory . Masters J.consensus and controversy – Models of Professional Roles . Karunya University  Page 650  .Ignacimuthu. Ltd. Rastogi Publications. International conventions patents and methods application of patents.S. Total : 65 hours Text Books 1.. Dubey. Legal implications.variety of moral issued .School of Biotechnology and Health Sciences Department of Biotechnology (BT) xenotransplantation. IPR and biosafety.uses of ethical theories Unit-II Environmental Aspects Of Biotech Applications (13) Use of genetically modified organisms and their release in environment Special procedures for r-DNA based product production.customs and religion . LC50. Oxford and IBH Publishing Co.moral dilemmas moral autonomy .2008 2. Animal Cell Culture: Practical Approach. Bains Biotechnology from Ato Z. Biodiversity and farmers rights. • Moral and Social Values and Loyalty • The rights of others Unit-I Engineering ethics (13) Senses of “Engineering Ethics”' .types of inquiry . Beneficial applications and development of research focus to the need of the poor. Satler et al Plant Biotechnology. aquaculture etc. the students would have learnt about • Engineering Ethics and Human Values. S.

TrEMBL _ structural databases – PDB.School of Biotechnology and Health Sciences Department of Biotechnology (BT) Unit-IV Global Issues (13) Multinational corporations . Databanks – nucleotide databanks – Genbank. UNIT II : Sequencing Alignment and Dynamic Programming (13) Introduction – Strings – Edit distance two strings – string similarity local alignment -gaps – Parametric sequence alignments – suboptimal alignments – multiple alignment – common multiple alignment methods. SCOP. DDBJ – protein databanks – sequence databanks – PIR. 1993.2007. “Professional Ethics and Human Values”.Dhinesh Babu. UNESCO Publications. Reference Books: S. Unit-V The Legal And Socioeconomic Impacts Of Biotechnology (13) Public education of the process of the processes of biotechnology involved in generating new forms of life for informed decision making Biosafety regulation and National and international guidelines r-DNA guidelines.weapons development engineers as managers-consulting engineers-engineers as expert witnesses and advisors -moral leadership-sample code of Ethics (Specific to a particular Engineering Discipline). 1988. telnet. 09BT227 BASICS OF BIOINFORMATICS Credit : 4:0:0 Objective: To impart knowledge on basic techniques of Bioinformatics Outcome: At the end of the course. UNESCO Publishers. EMBL. NCBI.Environmental ethics . Sasson A. “Biotechnologies and Development ". Unit III : Sequence Databases and Their Uses Karunya University  (13) Page 651  . levels of containment Total : 65 hours Text Books: 1. the students would have learnt about • Sequencing Alignment and Dynamic Programming • Sequence Databases • Evolutionary Trees and Phylogeny Unit I Introduction to Bioinformatics (13) Scope of Bioinformatics – Elementary commands and Protocols. Lakshmi Publications Pvt. Biosafety. 2. " Biotechnologies in developing countries present and future ". http. Experimental protocol approvals.computer ethics . ftp. Ltd. SWISSPROT. Sasson A. CATH. Printer on information theory.

1997. Reference Books 1.Parry Smith. EMBL – Nucleotide sequence database 4. Cambridge University Press. Total : 65 hours Text Book 1.2002. Introduction to Bioinformatics. Eukaryotic gene prediction 8. Identification of disease gene 9.Pearson Education.R. Biological Sequence analysis.Attwood and D. SWISSPROT/TREMBL – Protein sequence database 5. Lesk. R. 2.School of Biotechnology and Health Sciences Department of Biotechnology (BT) Introduction to databases – database search – Algorithms issues in database search – sequence database search – FASTA – BLAST – Amino acid substitution matrices PAM and BLOSUM.2001. 09BT228 BIOINFORMATICS LAB Credit : 0:0:2 List of Experiments: 1.Durbin.Cambridge University Press. NCBI .eddy. 1997 2. Algorithms On Strings Trees And Sequences. UNIT V : Special Topics in Bioinformatics (13) DNA Mapping and sequencing – Map alignment – Large scale sequencing and alignment – Shotgun – DNA sequencing – Sequence assembly – Gene predictions – Molecular predictions with DNA strings. T. BLAST – Similar DNA sequences search 3.K. Analysis of Protein sequence using PIR database 6. Analysis of structural features of proteins using protein data bank and SWISS PDB viewer 7. Protein sequence analysis tools 10.Viral and bacterial genome analysis 2.Graeme. Dan Gusfield.K.Probabilistic models of proteins and Nucliec acids. GCG Sequence Analysis(Basic concepts only) UNIT IV : Evolutionary Trees and Phylogeny (13) Ultrasonic trees – parsimony – Ultrametric problem – Perfect phylogeny – Phylogenetic alignment – connection between multiple alignment and tree construction.Anders and M. Multiple sequence alignment and phylogenetic trees Karunya University  Page 652  . Oxford university press . Introduction to Bioinformatics.

Gibbs Duhem equation. ideal and non-ideal solutions. concepts of chemical potential and fugacity. Karunya University  Page 653  . thermodynamics efficiency of growth. Maxwell’s relations and applications. stochiometric and energetic analysis of cell growth and product formation-elemental balances. Chemical And Engineering Thermodynamics. yield coefficients. v-l-e calculations for binary and multi component systems. degree of reduction concepts-available –electron balance. power cycle. composition models. concepts and applications of excess properties of mixtures. 2001. UNIT – IV Thermodynamic Analysis of Processes (13) Concept of lost work. Narayanan K. UNIT – I Thermodynamic Properties of Fluids (13) Volumetric properties of fluids exhibiting non ideal behavior. liquidliquid equilibria and solid-solid equilibria. Total: 65 Hours Text Books 1. Sandler S. UNIT – II Solution Thermodynamics (13) Partial molar properties. UNIT – V Biochemical Thermodynamics ( 13) Energetics of metabolic pathways energy coupling (ATP and NADH). residual properties. Prentice Hall India. John Wiley. Equilibrium criteria for homogeneous chemical reactions. UNIT – III Phase and Chemical Reaction Equilibria (13) Criteria for phase equilibria. activity coefficient.1989. effect of temperature and pressure on equilibrium constant. calculations involving actual property exchanges. A Text Book Of Chemical Engineering Thermodynamics.I. Reference books 1. oxygen consumption and heat evolution in aerobic cultures. calculation of equilibrium conversion and yields for single and multiple reactions. entropy generation. aim advantage will be to deal with power production and refrigeration processes.School of Biotechnology and Health Sciences Department of Biotechnology (BT) 09BT229 CHEMICAL THERMODYNAMICS AND BIOTHERMODYNAMICS Credit: 4:0:0 Objective The course aims at making the students understand the fundamental principles and concepts of chemical and biothermodynamics. The study further provides a comprehensive exposition to theory and application of solution thermodynamics. evaluation of equilibrium constant. estimation of thermodynamic properties using equations of state.V. Outcome The students will be well versed with the behavior of fluids under PVT conditions and also apply them for practical purpose. liquefaction. calculation of real irreversible processes.

variable – volume reactor [Integral method. 6th Edition. McGraw-Hill. Abbot M. definition of reaction rate. Smith J. CSTR and PFR.C. Collision Theory]. Unit-4 Multiple reactions (13) Irreversible reaction & reversible reactions. variable affecting the rate of reaction. Outcome To expose the fundamental of • Kinetics of homogeneous reaction in batch reactors • Design of ideal reactor • Design principles in non isothermal reactions • Non ideal flow reactor modules • Design equation in biochemical reactors Unit-1 Homogeneous Reactions (13) Introduction. Half life time. Total: 65 Hours Text Books 1. Multiple reactor. first-order followed by zero-order reaction.School of Biotechnology and Health Sciences Department of Biotechnology (BT) 2. reactor design.M. Kinetics and Energetics in Biotechnology. Dispersion model. CSTR. Tank in series model]. Third edition – John Willey. CSTR. Unit-2 Ideal reactors Types of reactor – Batch. Differential method]. molecularity & order of reaction.Rate equations . Batch reactor data / Kinetic data – Constant – volume reactor. Elseviers.Design Equations.Cell growth . Octave Levenspiel-“Chemical Reaction Engineering”.M. temperature dependent term of a rate equation [Arrhenius law. (13) Unit-3 Non – Ideal reactors (13) Non-ideal flow introduction & various models [Compartment model. mixed reactors. Denbigh reactions-Batch. Homogeneous reactions. 2001.. JA. rate expression. Karunya University  Page 654  . RTD studies. PFR connected in series & parallel. 1999.Stoichiometry . PFR. Unit-5 Biochemical Reactions (13) Biochemical Reactions . Van Ness H. Royels. 1983 09BT230 BIO REACTION ENGINEERING Credit : 4:0:0 Objective This deal with the study of kinetics of chemical and bio chemical process and exposes the design of several types of reactions. rate constants. 3. Chemical Engineering Thermodynamics. series & parallel reactions.. classification of chemical reactions.Mass Balance . zero-order followed by first-order reaction.

Karunya University  Page 655  . 1995. M. Application of knowledge of protein in biotechnology. Enzyme Kinetics 2. Reference Books 1. 1986. Growth Kinetics of E. Appreciate the application of this knowledge in protein engineering.School of Biotechnology and Health Sciences Department of Biotechnology (BT) 2. 4. Charles D. Outcome Upon successful completion of this course.. Design Equation of Batch Reactor 9.coli in Batch Reactor 8. 1990. 1992. Shule & Karg – “Bioprocess Engineering” – Prentice Hall. the student should be well versed in the following: Chemical bonds and their influence in protein structure formation. H – “ Elements of Chemical Reaction Engineering” – second edition – Prentice Hall of India Pvt. Design Equation of PFR 11. – “Chemical Engineering Kinetics” – Second edition – McGraw Hill. Scott Fogler. Ltd. 3. Medium Optimization by Plackett-Burman Method 6. 09BT231 ADVANCED BIOPROCESS LAB Credits : 0:0:2 List of Experiments: 1. second edition – McGraw Hill. Amino Acid Production 5. Holland – “Fundamentals of chemical Reaction Engineering” Second edition – John willey & sons. 2. Immobilization of Cells or Enzymes by Encapsulation 09BT232 PROTEIN ENGINEERING Credits: 4:0:0 Objective To develop skills of students in chemical bonds and their interactivity. Thermal Death Kinetics 12. 1981. Bailey & ollis – “Biochemical Engineering Fundamentals”. Enzyme Production by SMF 3. Comprehensive knowledge of 3-D structure in relation to its functions. protein architecture and its influence on protein function. Enzyme Production by SSF 4. Determination of kLa by Dynamic Gassing Out Method 7. Design Equation of CSTR 10. Smith J.

“Protein Engineering Handbook”.2003 09BT233 PLANT BIOTECHNOLOGY Credits: 4:0:0 Objectives To develop skills of the students in the area of plant biotechnology and its applications. alpha-beta-alpha. Drug Designing (13) Total : 65 hours Text Books: 1.”Protein Engineering and Design”. infrared.Tooze. formation of complexes.2009 4.Carey.Wiley VCH. International Edition. chymotrypsin and elastase UNIT V METHODS OF PROTEIN ENGINEERING Methods of Proteins engineering. Stefan/Bornschever. Secondary structure: Alpha. Quaternary structure: Modular nature. solubility. Immunoglobulins: IgG Light chain and heavy chain architecture. thiol.1996 3. Helix-turn-Helix motif in DNA binding. Paul R.1993.School of Biotechnology and Health Sciences Department of Biotechnology (BT) UNIT I BONDS AND ENERGIES IN PROTEIN MAKEUP (13) Covalent. Membrane proteins. Leucine zippers.Academic Press. “Proteins” Freeman WH Second edition. Creighton TE. UNIT III PROTEIN ARCHITECTURE (13) Primary structure. carboxyl. up and down & TIM barrel structures nucleotide binding folds. micro. hydrophobic and Vander walls interactions in protein structure. beta-sheets. folding. Karunya University  Page 656  . Ionic. Lutz. visible. Chemical reactivity in relation to posttranslational modification (involving amino. ultraviolet. charge.“Harpers’s Illustrated Biochemistry” 26th Edition. denaturation and renaturation. abzymes and Enzymes : Serine proteases. Tertiary structure: Domains.1993 2. Interaction with electromagnetic radiation (radio. Immunotoxins. X-ray) and elucidation of protein structure. UNIT II AMINO ACIDS AND THEIR CHARACTERISTICS (13) Amino acids (the students should be thorough with three and single letter codes) and their molecular properties (size. Moody PCE and Wilkinson AJ “Protein Engineering” IRL Press. topology diagrams. imidazole groups) and peptide synthesis. Garland. Reference books: 1. understanding catalytic design by engineering trypsin. Zn fingers. Coordinate. Robert Murray. beta and loop structures and methods to determine Super-secondary structure: Alpha-turn-alpha. Oxford.1990 2. Daryl Granner. pKa). Hydrogen. beta-turn-beta (hairpin). “Introduction to protein structure”. UNIT IV STRUCTURE-FUNCTION RELATIONSHIP (13) DNA-binding proteins: prokaryotic transcription factors. Branden C. Eukaryotic transcription factors. helix-turn helix motifs in homeodomain. hydroxyl.

Germplasm conservation. Micro propagation. Mathew JA.. Nigel Scott. 2. Pests and insects resistance.S. Ltd.. Regulation of NIF and NOD Genes. Mickee RA. Camv. Chawala. factors influencing disease resistance and susceptibility RFLP Unit 5: Transgenics (13) Stress tolerance-Biotic and a biotic temperature. New Delhi 2002. Pvt. legume symbiosis. Oxford and IBH Publishing Co. Gene Banks. BBTV. Pvt. major plant viruses. Gemim viruses etc. and Mark Fowler. Plant as Bioreactors. The genetic manipulation of plants. Types of Methods in plant Biotechnology. Introduction to Plant Biotechnology.development of disease resistance plants by introducing Bacillus thuringiensis genes. S. Callus Culture.viral resistance. 2004. Ignacimuthu S.edible Vaccines. An Introduction to Genetic Engineering in Plants. Culture Medias. N2 Fixation. Principles of Plant Biotechnology. Plant viruses and Genetic Engineered viruses as a tool of deliver foreign DNA. H. Reference Books 1. Blackwell Scientific Publication. salinity. Agro bacterium types. Plant Vectors and plant Viruses (13) Vectors and Genetic Engineering. Hairy Root Culture. Adrian Slater. Somatic Embryogenesis. drought etc. Unit 3: Application of Plant Biotechnology (13) Hairy Root Cultures and Secondary Metabolite production.. Total : 65 hours Text Books 1. Mantal SH. Oxford and IBH Publishing Co. 2000. TMV. Crop improvement.School of Biotechnology and Health Sciences Department of Biotechnology (BT) Outcome At the end of the course. Oxford University Press 2008. Unit 4: Molecular Aspects of Disease Susceptibility and Resistance (13) Transposable elements. Ltd. 2. Unit 2: Plant Genetic Engineering Tools. Agro bacterium mediated gene transfer and cloning. Cell and Tissue Culture.. the students would have learnt about various Plant tissue culture methods and its applications Unit 1: Cell and Tissue Culture (13) Definition and need. Karunya University  Page 657  .J. New Delhi. Plant Biotechnology. Plant Biotechnology.

animal diseases. UNIT IV Micromanipulation Of Embryos (13) What is micromanipulation technology. stem cell cultures in the production of transgenic animals. UNIT V Transgenic Animals (13) Concepts of transgenic animal technology. Total : 65 hours Karunya University  Page 658  . equipments used in micromanipulation. micromanipulation technology and breeding of farm animals. Outcome At the end of the course. molecular diagnostic techniques like PCR. UNIT II Animal Diseases And Their Diagnosis (13) Bacterial and viral diseases in animals. strategies for the production of transgenic animals and their importance in biotechnology. the students would have learnt about various animal tissue culture methods. RFLP. transgenic animals and its applications UNIT I Animal Cell Culture (13) Introduction to basic tissue culture techniques. chemically defined and serum free media. in-situ hybridization.School of Biotechnology and Health Sciences Department of Biotechnology (BT) 3. Dodds JH. 09BT234 ANIMAL BIOTECHNOLOGY Credit : 4:0:0 Objective To develop skills of the students in the area of animal biotechnology and its applications. gene therapy for animal diseases. northern and southern blotting. in vitro fertilization and embryo transfer. UNIT III Therapy Of Animal Diseases (13) Recombinant cytokines and their use in the treatment of animal infections. their maintenance and preservation. animal cell cultures. monoclonal antibodies in therapy. somatic cell fusion. artificial insemination and germ cell manipulations. enrichment of x and y bearing sperms from semen samples of animals. micromanipulation. monoclonal antibodies and their use in diagnosis. Cambridge University Press. organ cultures. various types of cultures. continuous flow cultures. therapy. 2001.suspension cultures. immobilized cultures. cell cultures as a source of valuable products. Plant Genetic Engineering. vaccines and their applications in animal infections.

Macdonald F and Ford CHJ. 2.W. Cancer screening and early detection. principles of physical carcinogenesis. activation of kinases. Blackwell Publishers.School of Biotechnology and Health Sciences Department of Biotechnology (BT) Text Books 1. UNIT III : Principles Of Molecular Cell Biology Of Cancer (13) Signal targets and cancer. mutations that cause changes in signal molecules. modulation of cell cycle in cancer. The Biological Basis of Cancer. tumor markers. IRLl Press. effects on receptor. New York 2003. UNIT II : Principles Of Carcinogenesis (13) Theory of carcinogenesis. identification of oncogenes. Barry Pierce. Blackwell Scientific Publications. Molecular Biology of Cancer. Parchment. Ralph E. Karunya University  Page 659  . The Molecular Biology of Cancer. Chemical carcinogenesis. Perantoni. Oncogenes.J. Stella Pelengaris and Michael Khan. Telomerases. detection of oncogenes.2002. 2001. Alan O. signal switches. G. Bios Scientific publishers. Detection using biochemical assays. Cambridge University Press. An Introduction to Cellular and Molecular Biology of Cancer. diet and cancer. 2003. UNIT I : Fundamentals Of Cancer Biology (13) Regulation of cell cycle. 09BT235 CANCER BIOLOGY Credit : 4:0:0 Objectives To develop skills of the students in the area of cancer biology Outcome At the end of the course.B. x-ray radiation-mechanisms of radiation carcinogenesis. 3. J Oxford Medical Publications. retroviruses and oncogenes. Oncogenes/proto oncogene activity. the students would have learnt about fundamentals of cancer biology. 2006. metastasis and cancer therapy. molecular tools for early diagnosis of cancer. carcinogenesis. Dunmock N. Robert G.. “Introduction to Modern Virology”. Reference Books: 1. tumour suppressor genes. metabolism of carcinogenesis. different forms of cancers. Maly B. Oxford. Oxford. McKinnell.J And Primrose S. 2. 2002 4. “Virology A Practical Approach”. Growth factors related to transformation.

East West Affiliated Press.Project profile concept-details. detection of cancers. Preparation of Budgetory investment and production costs. A Text book of Biotechnology. Process Economics. 2002 2. S. prediction of aggressiveness of cancer. Gupta P.D. Ramadass P.W. heterogeneity of metastatic phenotype. Fixing most economic processes. Market Survey. Akshara Printers. Dubey. Gene therapy. three step theory of invasion.R. 2004. Kumar H. Sourcing of Processes. Chand & Co. Oxford University Press. 2003. proteinases and tumour cell invasion. Agrobios India Limited. R. Meerut. Elements of Biotechnology. Structure and Types of Organizations UNIT II : Project Design And Development (13) Choosing a Project. advances in cancer detection. 2000. Reference Books: 1. metastatic cascade. Animal Biotechnology. Importance of various M-inputs-Globalization concept-Competition by Dumping-It’s effect on Plant size-Status of India. Meera Rani S. 2. 4.School of Biotechnology and Health Sciences Department of Biotechnology (BT) UNIT IV : Principles Of Cancer Metastasis (13) Clinical significances of invasion. Process alternatives. Karunya University  Page 660  .C. Ranga M. 09BT236 BIOPROCESS ECONOMICS AND PLANT DESIGN Credit : 4:0:0 Objective To know about the various Bioprocess and the ways to enhance the production of industrially important bioproducts Outcome At the end of the semester the students will be able to analyze various bioprocess. reactor design and control .M. 2004. Process Flow sheets. Importance of Techno-Economic-Feasibility Studies. Use of signal targets towards therapy of cancer. chemotherapy. Text Book of Biotechnology. UNIT V :New Molecules For Cancer Therapy (13) Different forms of therapy. radiation therapy. Masters J.K. Plant Location Principles. Animal Cell Culture: Practical Approach. Total : 65 hours Text Books 1. Plant Lay out. TechnologyScanning. 2000. 3. basement membrane disruption. Text Book of Animal Biotechnology. UNIT I : Process Economics And Business Organizations (13) Definition of Process. Rastogi Publications.

Economic-Balance. the students would have learnt about the advantages of deriving fuel through fermentation rather than from fossil sources. To know about water treatment and sludge treatment methods. Describe the part played by bacteria in the process of decay and recycling of carbon and nitrogen. Simple SPC concept details. Environmental Pollution . Types and causes and its effects on environment. Unit I : Introduction (13) Origin and Definition of old and new biotechnology. which can be exploited to save environment from eventual deterioration. Lakshmi Publications.1991 2. Rudd Watson. Estimation of Capital costs. Peter MS . Concept of time-Value of money.”Stratergy for Process Engineering”. Balance Sheets. Annual reports. Project profitability evaluation. Depreciation.1987 09BT237 APPLIED ENVIRONMENTAL BIOTECHNOLOGY Credit : 4:0:0 Objective: To develop skills of students in various biological phenomena. Senapathy. Source Sink concept of Profitability. UNIT V : Quality And Quality Control (13) th Current good manufacturing practices.Wiley Publications.”Textbook of Principles of Management and Industrial Psychology. Fundamental Concepts of ISO 9000 Quality System and the various requirements for ISO certification. Profitablity Standards. scope and importance of Biotechnology in India.soil. Manufacturing Costs. Elements of quality control envisaged by BIS since 1947. Alternative investments and Replacements. water air. Profitability And Accounting (13) Capital investment.School of Biotechnology and Health Sciences Department of Biotechnology (BT) UNIT III : Cost Estimation.Chemical Engineering Series.Klaus D. oil and heavy metal pollution.Mc Graw Hill. Performance Analysis. Karunya University  Page 661  . Six sigma concept in Quality control Total : 65 hours Text Books: 1. Concepts of Quality Control in 20 century. Emergence of Statistical Process Control (SPC).2001 Reference Books: 1. Microbial flora of soils . UNIT IV : Process Optimization Techniques (13) Optimum design-Design Strategy. Design of Variables. Capital Costs. “Plant Design and Economics for Chemical Engineers. Biotechnology a multidisciplinary growing tree. integrate prior knowledge of how biophysical systems work so as to better understand the constraints and opportunities for naturalresource and environmental management.International Edition. Working Capital. To understand the process of bioremediation Outcome: At the end of the course.

S. Biotechnology and Biodegradation. microbes in leaching of metals – leaching of copper and uranium. cellulose as a source of energy. 4th edition. W. Biological nitrogen and phosphate removal . Ellis.2008 2. Vol. London 2007.Environmental Biotechnology : Principles and Applications. Loss of biodiversity and its causes. waste as renewable source of energy.waste water treatment in diary. Unit IV : Bioremediation and biodegradation (13) Pseudomonas for bioremediation. Karunya University  Page 662  . Chakrabarty. types and reactions of bioremediation. Honwood Ltd. 2007 Reference Books: 1. Chakrabarty K. proteomics and its application This will be very useful for the student to undertake research /project work inbioinformatics. biodegradation of hydrocarbons and pesticides. Unit III : Biomass (13) Types and composition of biomass. methods of energy production.Chand publications. R. 2. Unit V : Biodiversity (13) Biodiversity at global level. Control of air pollution.A. Springer. control devices for gaseous pollutants. Anderson and A. sewage disposal and treatment – physical and biological treatment. tannery. Dubey A Textbook of Biotechnology. Intellectual Property Rights (IPR) and patenting – patents for higher plants and animals Total : 65 hours Text Books 1. 2007 09BT238 INTRODUCTORY GENOMICS AND PROTEOMICS Credit : 4:0:0 Objective To develop skills of the students in the area of genomics and proteomics.A. Moo-Young. Foster C. effluent treatment – primary and secondary treatment. energy and fuel using microorganisms. the student would learnt about various aspects of genomics. .New Delhi. Environmental Biotechnology.S. species diversity.Biodiversity and its conservation – insitu and exsitu conservation.M.F. John ware D. conversion of methane to synthetic gas. Outcome At the end of the course.Karnely D. Gulf Publications Co. volatile organic pollutants. Advances in Applied Biotechnology series. M. sugar and industry pulp.School of Biotechnology and Health Sciences Department of Biotechnology (BT) Unit II : Industrial waste water management (13) Aerobic and anaerobic waste water treatment. C. conservation of energy. Ovnen G. distillery.

Unit IV: (13) Introduction to Proteome . T. 2nd Edition. chromosome walking. BIOS Scientific Publishers Ltd. Some specific metabolic databases Total: 65 hours Text Book: 1. Contig assembly. Viva Books Ltd. BLOCKS. Expression analysis – micro array approach. high through put method – Automated sequencing methods Unit II: (13) Various Approaches in sequencing genome – shotgun. PROSITE. Outcome At the end of this course. David W Mount. quality control and quality assurance of pharma products etc. Unit V: (13) Proteome databases: protein sequence database.information and the proteome – Primary attributes for protein identification . 2002 Reference Book: 1. 2nd edition. specialized protein sequence databases. Radiation hybrids – Sequence markers. chromosome jumping. directed short gun & Clone contig approach – Primer walking. 2002 2. Human Genome Project Transcriptome– structural & functional Genomics – comparative & population genomicsPharmacogenomics – Phylogenetics. biopharmaceutical products. Genome Marking and mapping techniques Unit III: (13) Mapping and sequence assembly – genetic marker– Physical Marker –. Metabolic databases. 2-D PAGE databases.A Brown .Protein N.protein species of origin . 2004 09BT239 BIO PHARMACEUTICAL TECHNOLOGY Credit: 4:0:0 Objective To develop skills of students in the area of biopharmaceutical technology. the students would have learnt about pharmacokinetics and pharmacodynamics principles.cross species protein identification . important unit operations. SWISS-PROT.and C-terminal sequence tags . OMIM. Karunya University  Page 663  . genomic databases. Nucleotide & protein sequencing methods – Chemical. Pennington. 2nd edition.Modifications that influence protein change on 2-D PAGE . Proteomics from Protein Sequence to Function. TrEMBL. enzymatic.School of Biotechnology and Health Sciences Department of Biotechnology (BT) Unit I: (13) Overview –Variation and regulation in Prokaryotic & Eukaryotic genome expression profile– Brief outlook of various genome projects and their outcome.proteome and technology . PDB. Bioinformatics: sequence and genome analysis. CBS publishers. Genome.

Gilman. UNIT III Preparation Of Semisolid And Liquid Dosage Forms (13) Manufacturing Principles .Anamaya Publishers.Drug Metabolism – Physico-Chemical Principles –Pharmacodynamics – Action of drugs on human bodies. Popovich.2005 . Chemoinformatics. Packaging of semisolid and liquid dosage forms UNIT IV Pharmaceutical Products (13) Pharmaceutical Products . Drug Metabolism and Pharmacokinetics .Clinical Trials – Design. Good manufacturing practice. 2005. Limbird.highlight. UNIT II Preparation of Solid Dosage Forms (13) Manufacturing Principles . Longman.Vitamins – Cold remedies – Laxatives –Analgesics –External Antiseptics – Antacids. Essential of Medical pharmacology by. N. double blind studies. Recent advances in the manufacture of drugs using r-DNA technology.2005 A.Pharmaceutical Dosage Forms and Drug Delivery Systems. L. 1. 4. Biopharmaceutics and Pharmacokinetics-A Treatise..Lee E.Compressed tablets – wet granulation – Dry granulation – Direct compression – Tablet presses formulation – Coating – Pills – Capsules sustained action dosage forms. QSAR and Molecular Modeling. 5. lippin cott williams & wilkins publishers. FDA regulations (General) and Indian Drug regulations. Alfred G. UNIT V Clinical Trails And Regulations.Parental solutions – Oral liquids – injections – Ointments. 2. 1. Karunya University  Page 664  . Satya Prakash Gupta. Sunil B Jaiswal . Allen. Packaging of solid dosage forms. Goodman & Gilman's The pharmacological basis of therapeutics by Joel Griffith Hardman . Springer ..Leach. 1999.Development of Drugs and Pharmaceutical Industry.. Cerius 2 and QSAR to Discover and Design New Molecules. Molecular Modelling Principles and Application. Williams & Wilkins.R. Reference books Remington’s Science and Practice of Pharmacy. 2003 09BT240 NANOBIOTECHNOLOGY Credit: 4:0:0 Objective To develop skills of the students in the field of nanobiotechnology and its applications in various fields. Ansel. Quality control tests for semisolid and liquid dosage forms. Bioinformatics (13) Clinical Trials & Regulations . 2. 3. 2008.School of Biotechnology and Health Sciences Department of Biotechnology (BT) UNIT I Basic principles of Pharmacokinetics and Pharmacodynamics (13) Introduction . Quality control tests for tablets and capsules. placebo effects. Vallabh prakashan. Total: 65 hours Text books DM Brahmankar. Tripathi KD. Antibiotics – Biologicals – Hormones. 1996. H. The Use of Molecular Modelling.

UNIT 2 (13) Nano Particles :Introduction. Dental care. Ratner M. Social and Ethical Issues. Soft Lithography. Benefits and challenges in Molecular manufacturing: The Molecular assembler concept. Scope and Overview.Applications in cancer biology. Alumina. Synthetic retinyl chips based on bacteriorhodopsins. Nanotechnology and Future Socio-economic Challenges Total:65Hours Text Books 1. Reference Books Karunya University  Page 665  . Economic Impacts. Fabrication of Novel Biomaterials through molecular self assembly. History of Nanotechnology. Dental care. Silica. Nanosurgical devices. Length scales . Nanocomputers. Ethical Issues in Nanotechnology: With Especial Reference to Nanomedicine. Atomic Force Microscopy. 2004. Nano wires and Nanotubes. 2. Cobalt. Nalwa – “Encyclopedia of Nanoscience & Nanotechnology” McGraw Hill. Toxic effects of Nanomaterials. Nanocomputers. Iron oxide. Materials. Zinc oxide. UNIT 1 (13) Introduction. Visions and Objective of Nanotechnology. Socioeconomic Challenges. Nanocrystals in biological detection. Gold. PDMS Molding. Other Issues. Power storage. Social Issues Relating to Nanomedicine. 2003. Nanobiotechnology. Artificial neurons. Ratner D – “Nanotechnology: A Gentle Introduction to the next Big idea”. Characterization of Nanoparticles. Fields: Automobile. Techniques to Synthesize Nanoparticles. Nano-Biodevices and Systems. Nano Particles. UNIT 3 (13) Application: Nanomedicine. Types of Nanoparticles. the students would have learnt about Automobile. S. Significance of Nanoparticles Nanofabrications. Nanobiotechnology. Understanding advanced capabilities. Electronics. Real-time nanosensors. Electronics. Silver. Electrochemical DNA sensors and Integrated Nanoliter systems. Future of Nanotechnology: Nano Technology Revolution. Nanotechnology products Latest Developments in Nanotechnology etc. Importance of Nanoscale and Technology.MEMS/NEMS.Future nanomachine. Prentice Hall. Materials. Silicon based Technology.School of Biotechnology and Health Sciences Department of Biotechnology (BT) Outcome At the end of the course. High throughput DNA sequencing with nanocarbon tubules. Nanobiocensor and Nanofludics. Medicine. Controversies and confusions. Nanomedicine Applied in Nonmedical Contexts. Pure Metal. Nanomedicine. Titania. UNIT 5 (13) Ethical Issues in Nanotechnology: Introduction. UNIT 4 (13) Nanobiotechnology: Clinical applications of nanodevices.pen Nanolithography. Applications. Metal Oxides. Self assembled monolayers/ Dip. Silicon. Nanotechnology in Different.Small scale systems for in vivo drug delivery. Medicine. This will serve as an effective course to understand Socio-economic issues of nanobiotechnology. H.

gene bank.. Paper Chromatography Differential Scanning Colorimetry 09BT242 INTRODUCTORY BIOTECHNOLOGY Credit 3:0:0 Objective To develop skills of the students in the field of biotechnology and its applications in various fields. Greco. John Willey & Sons Ltd. Agarose gel Electrophoresis 10. X-ray Diffraction instrument 12. criminology & taxonomy. Finding the Concentration of Sodium and Calcium using Flamephotometer 4. .. 4.Gene cloning vectors – Plasmids PBR 322. finger printing. CNR Rao – “ The chemistry of Nanomaterials: Synthesis. Lyophilizer 9. molecular characterization etc. PUC 8 – cosmids PLFR 5. PJB 8 – SV40. 2008. UV spectra of Nucleic Acids 2. Third edition. Goodsell – “ Nanobiotechnology”. Western Blotting 6. This will serve as an effective course to understand Socio-economic issues of Biotechnology UNIT – I Definition – Scope – Biotechnology in India – Applications of biotechnology: disputed parenthood. Outcome At the end of the course. Viola Vogel –“ Nanomedicine & Nanotechnology”. Ralph S. Karunya University  Page 666  . HPLC 7. CRC Press.School of Biotechnology and Health Sciences Department of Biotechnology (BT) 1. the students would have learnt about the cloning. 2005 09BT241 ANALYTICAL TECHNIQUES IN BIOTECHNOLOGY LAB Credit: 2:0:0 List of Experiments 1. 2004.“Nanoscale Technology in Bilogical systems”. Chromatography analysis using TLC 3. UNIT – II Principles and Methods of gene cloning – application. 3.amplification – expression vectors. John Willey & Sons Ltd. Properties & Application” Springer 2006 2. GC 8. transgenics. BPV – their salient features and uses – enzymes in gene cloning – transfection . PCR 5. Nephlometer 11.

S. M. Phosphorous.Robert Koch.History and recent developments . S. New Delhi. Genetic Engineering. R. 2004. This will serve as an effective course to understand bioremediation of microbes and manipulation of microbes for our benefit. Chand & Co. 1989. Text Book of Biotechnology. Unit I : Definition and scope of Microbiology . University Press (India) Ltd. S. history. Biological Databases and Websites. UNIT – IV Integrated nutrient supply and IPM system. Chopra.School of Biotechnology and Health Sciences Department of Biotechnology (BT) UNIT – III Transgenic plants – herbicide. Text book: 1. microbial interaction. Chand & Co.C. U. R. Sulphur – Solubilization.. John Tyndall. Reference Books: 1. Concepts of Biotechnology. fishes and poultry.Leewenboek. Biological nitrogen fixation – basic requirements – symbiotic and nonsymbiotic nitrogen fixation – production and application of rhizobium as a biofertilizer – production and application of ethanol as bio--fuel. 3. D. Outcome At the end of the course. 1996. enzyme production etc.spontaneous generation . biofertilizers. 2004. 4. Joseh Lister . insecticide and virus resistant plants – Transgenic animals mice. Genetic Engineering and Biotechnology. Viswanathan & Co.C. Potash. Unit II : Karunya University  Page 667  . Hyderabad.Biogenesis-contributions of Louis Pasteur . UNIT – V Gene Banks – importance – DNA finger printing – methodology and application –methods of gene therapy – biosensors – types and application of biochips. 2. Lazaro Spallanzani. Balasubramania. 1996. 09BT243 APPLIED MICROBIOLOGY Credits: 3:0:0 Objective To develop skills of the students in the field of microbiology to understand the basics and recent developments. Culturing methodology and sterilization techniques. Oxford & IBH. A. Socio-economic issues of Biotechnology. Dubey. Dharmarajan. and Narim.L. Text Book of Biotechnology. the students would have learnt about the scope. Dubey. cattle.

Kalyani Publishers.. cytoplamic membrane and cytoplasmic inclusions.L.mode of action .Applied Microbiology. organization & reproduction of bacteria. Reference Books: 1. S.Phase contrast . Ingrahm. WCB Mc Graw Hill. M. Microbiology 5th Edition. slime layer. New York2002. Vitamins – Cyanocobalamin. Preservation of food.A. the students would have learnt about the functions of organelles and structure.C. 2006 2. Harley.classification . fungi. R.P.source . A Text Book of Microbiology . 1998 5.R Kreig Microbiology 5 th edition.S Chan and N. 4. Unit V: Sewage disposal and treatment-Physical and biological. viral. Outcome At the end of the course.J.Applied Microbiology. M. fungal pathogens.. yeast. Singh.structure and function of cell wall. New Delhi.R. flagella.Dark ground .Binomial nomenclature of microbes Unit IV: Host microbe interaction. capsule.Y.Antimicrobial resistance .Fluorescence and Electron Microscopy.A.Prescott. 2007. Himalaya Publishing House. General Microbiology by H. pili. cilia. D. A Text Book of Microbiology . M. E. Painter.R. sporulation.Five kingdoms . J. bacterial. R.J. Wheel is & P. Microbes in the production of organicacids – Lactic acid. plant Karunya University  Page 668  .Tests for sensitivity to antimicrobial agents and its quality control. 2007. Reddy.A. M. Methods of purification of air. antibiotics -Penicillin and alcoholic beverages – Beer and Wine. Tat McGraw Hill Publishing Company. 09BT244 INTRODUCTORY CELL BIOLOGY Credits: 3:0:0 Objective To develop skills of the students in the field of cell biology and its applications in various fields. New Delhi 2003 2.P and Klein. S. Pelczar M. Applied Microbiology. 2007 3.School of Biotechnology and Health Sciences Department of Biotechnology (BT) Anatomy of prokayotes and eukaryotes . Mumbai.L. Microbial kingdoms . Schlegel (7 th Edition) Cambridge University Press.R.. General Microbiology by Stanier. and Singara Charya. J.cell theory . compound light microscopy . Himalaya Publishing House. Enzyme – Amylase Text Book: 1. Antimicrobial chemotherapy Antibiotics . and Singara Charya. bacteriophage. Unit III : Microscopy: simple.G. Mumbai.Microbes in air. algae. Reddy.

E.Dornel J.Gerald Karp and Nancy L Puritt.School of Biotechnology and Health Sciences Department of Biotechnology (BT) viruses. 1999. cyclic AMP. E. Wesely Publishers. 1990 2. yeast. Saunders College.Toxins and their mode of entry into cell-Cholera and Diphtheria UNIT IV Receptors And Models Of Extra Cellular Signaling Cytosolic – Nuclear and membrane bound receptors Autocrine.D. 09BT245 INTRODUCTORY GENETIC ENGINEERING Credits: 3:0:0 Objective To develop skills of the students in the field of genetic engineering concepts and applications to build their knowledge mainly in manipulation of DNA. “Cell Biology”. Philadelphia. fungi. Freeman. “Cell and Molecular Biology” 6th Edition.. This will serve as an effective course to understand signal transduction and cyclic pathway of cells UNIT I Cell Structure And Functions Of Organelles Eukaryotic and Prokaryotic cells – Principles of membrane organization – membrane proteins (Internal and external) .F. Baltimore D. 3. Structure.Concepts and Experiment”. algae. role of inosital phosphates.Kimball I. Reference Books: 1. 1989.De Robertis. 1990. Inc.M. Lodish H. 2. paracrine and endocrine models of action – Quantitation and characterization of receptors UNIT V Signal Transduction Signal amplification pathways. John Wiley and Sons. Organization & reproduction of bacteria. 1998. “Molecular and Cellular Biochemistry”. UNIT II Cell division – mitosis and meiosis – cell cycle and factors that control cell cycle. G proteins and calcium ion role in cell signaling Text Books 1. “Molecular Cell Biology”. W. cyclic GMP. bacteriophage. UNIT III Transport Across Cell Membranes Passive and active transport – permeases – sodium – potassium pump ..W.P and De Robertis.H.. animal viruses etc. Stephen L.Wolfe. Outcome Karunya University  Page 669  . Inc. Wadsworts. plant viruses and animal viruses. “Cell and Molecular Biology . California.

W.Yeast expression system-Retroviral vectors-Bacullovirus. 2004 2. An Introduction to Genetic Engineering. Gene transfer techniques.A.1993 09BT246 ROLE OF BIOTECHNOLOGY IN ENVIRONMENT Credits: 3:0:0 Objective To develop skills of the students in the field of environmental biotechnology and its applications. DNA finger printing. This will serve as an effective course to understand bioremediation and biodegradation of wastes. 2004. the students would have learnt about the social issues of pollution.Primrose SB. S. Applications of rDNA technology in Diagnostics.Old R. Oligonucletoide derived mutagenesis. DNA sequencing. UNIT II Cloning Vectors-Prokaryotes Plasmid biology-Plasmids as vectors. blotting.Brown. transfectionRecombinant selection and sereening.cDNA libraryUNIT IV Expression Vectors Eukaryotic cloning vectors.C.1998 2. the students would have learnt about the cloning vectors. This will serve as an effective course to understand concepts and applications of recombinant DNA technology. Meerut.2001 3. DNA finger printing-RFLP Analysis-chromosome walking.PBR322.Ligases-Modifying enzymes Southern hybridization-Northern hybridization. . Site directed mutagensis-Its applications.pUC vectors-Other artifical plasmids-Phage-Lifecycle-M13-Lambda phages-Invitro packaging-Cosmids-Phasmids UNIT III Cloning Strategy Construction of recombinant DNA: Preparation of competent cell-Transformation. Outcome At the end of the course. Rastogi Publications. Mammlian cells-SV40.Gupta P.Genetic Engineering by Nicholas. Pathogensis. Chand & Co. Autoradiography. Text Book of Biotechnology. R. Genetic diversity. “Principles of Gene Manipulation.School of Biotechnology and Health Sciences Department of Biotechnology (BT) At the end of the course. UNIT I Basic Tools In Genetic Engineering Restriction enzymes.Eukaryotic expression vectors. waste water management. UNIT V Gene Modification & Application Of Recombinant DNA Technology Mutagenesis-Deletion mutagenesis.Backwell Scientific Publications. biodiversity and conservation etc.KElements of Biotechnology.Dubey. Reference Books: 1.Gene Cloning by T. Genomic DNA library. PCR-different kinds-Application.Derivatives of pBR 322. Karunya University  Page 670  . Therapeutic proteins-Vaccines Text Books: 1.. autoradiography etc.

Pollution. Biodiversity at Global Levels. diagnostic and therapeutic applications of enzymes. Unit I: Structure and Properties of Carbohydrates and Lipids Karunya University  Page 671  . 2006 2 Foster C. Ecosystem. Types. Johnware D. lagooning. Environmental Pollution. Environmental Biotechnlogy. COD and BOD sensors. proteins etc. Global Warming. Ozone depletion. Management of Sludge and Solid waste treatmentLand filling.F. lipids. Text Book: 1. Environmental Biotechnology. Hotspots in Biodiversity. Conversion of Solid Waste to Methane.Ellis Harwood Ltd. Species. Genetic. Social IssuesGreen House Gases. 09BT247 BASICS OF BIOMOLECULES Credits: 3:0:0 Objective To develop skills of the students in the field of structure and properties of biomolecules. 3 Gupta P. air. Acid Rain. S. Types. nuclic acids. 2004. oil and heavy metal. Biogas production. Causes and Effects of Soil.Ex situ and In situ Bioremediation.School of Biotechnology and Health Sciences Department of Biotechnology (BT) Unit 1: Environmental pollution and social Issues Definition and Scope of Environmental Biotechnology. Meerut. R. the students would have learnt about the structure and properties of carbohydrates. Unit 3: Biomass. control measures.C.Basic concepts and Applications. Reference Books: 1 Indu shekhar Thakur. Methods of energy production. Biodiversity and its Conservation. Types. Unit 2: Industrial Waste Water Management Purification of waste water. genetically Engineered Microbes for Bioremediation. Aerobic and anaerobic treatments. . Rastogi Publications. Elements of Biotechnology. Composting and Vermi Composting. Values of Biodiversity. 2004. This will serve as an effective course to understand the classification. Text Book of Biotechnology. 1987. Dubey. nuclear accidents and holocaust. Loss of Biodiversity and its causes threats to Biodiversity. Outcome At the end of the course. Chand & Co.In situ and Ex situ bio conservation Unit 5: Bioremediation and Biodegradation Definition. Management of radioactive pollutants in water. water. Energy and Solid waste Management Biomass waste as renewable source of energy. Unit 4: Biodiversity and Conservation Definition.A.K.

Prentice Hall International. 2nd Edition. UNIT I Structure Of Nucleic Acid Structure of DNA.School of Biotechnology and Health Sciences Department of Biotechnology (BT) Structure and properties of mono. dietary sources.. Outcome At the end of the course. R. This will serve as an effective course to understand the molecular basis of mutations. gluconeogenesis. Voet and Voet. Unit II: Structure and Properties of Proteins and Nucleic Acids Structure. AL. Avery. nucleic acids. the students would have learnt about the genetic materials.K. Vitamins: Classification. Hershey and Chase. classification. translational modifications.K. Text book: 1. Bioenergetics: redox biochemistry. Biochemistry. 2000. . oligo and polysaccharides. genetic code. Biochemistry. respiratory chain.. different forms of DNA and RNA.. Rodwell. androgen. P. Satyanarayana.A. Structure and properties of purines. Urea Cycle. Unit IV: Enzymes and Vitamins Classification. biochemical action and biochemical functions of estrogen. transcription. V.27th edition 2007. Unit III: Intermediary Metabolism and Bioenergetics Carbohydrate Metabolism: Glycolysis. glycogenesis.5th edition 2008 2. Structure. 4th Edition. Uppala Author Publishers Interlinks 2003 Reference Books: 1.. replication of DNA in eukaryotes. oxidative phosphorylation . Frankel and Singer. WH Freeman & Co. Principles of Biochemistry. energy rich compounds.U. 3. calcitrol (group I hormones). 2nd edition. diagnostic and therapeutic applications of enzymes.. 2. Dr.W. Lubert Stryer. Identification of DNA as genetic materialGriffith. TCA cycle. pyrimidines. properties. 09BT248 GENE AND GENE EXPRESSION Credits: 3:0:0 Objective To develop skills of the students in the field of genetics. Murray. Mayes. Lehninger. and properties of amino acids and proteins. cAMP. localization of proteins etc.. McLeod and McCarty. Granner.. FSH and LH (group II hormones). daily requirements and clinical orientation of water soluble and fat soluble vitamins Unit V:Hormones Hormones: Classification. di. Biochemistry. B. Meselson and Stahl experiment Karunya University  Page 672  . functions. Nelson DL and Cox MM. John Wiley & Sons Inc. classification and properties of fatty acids and steroids. 3rd edition 2008. Harper’s Biochemistry.

DavidFriefelder. beverages (beer. Telomeric DNA replication UNIT III Expression .S. Gupta P. recover/uses and mode of action – enzymes. different types of mutation. 2004. Steitz JA.Transcription And Translation Synthesis and processing of different forms of RNA. aerobic and anaerobic bacteria. algae and fungi. citric acid). 2004. Hopkins WH. S. lactic acid.A Brown. alcohol (ethanol). UV repair mechanisms. S.School of Biotechnology and Health Sciences Department of Biotechnology (BT) UNIT II Replication Of Nucleic Acids Semi conservative replication. Molecular basis of mutation. 2002. Text Book of Biotechnology. Unit – II: Microbes in pharmaceutical. Karunya University  Page 673  . 2nd Edition. Elements of Biotechnology. Reference Books 1. lac.C.Ignacimuthu. Watson JD. 3. Dubey.K. different models of replication in prokaryotes.NarosaPubl. BIOS Scientific Publishers Ltd.J. biofertilizer. viruses. Ltd. amino acids. Outcome At the end of the course. UNIT IV Splicing And Mutation RNA splicing-retroposons. the students would have learnt about the interaction of micro organisms in the field of pharmaceutical. 2004.”MolecularBiology”. replication of DNA in Eukaryotes. agricultural. their importance-Genetic codeTranscription-Eukaryotes & Prokaryotes. bacterial growth curve. Pvt. This will serve as an effective course to understand the biomass energy production Unit – I: Introduction to Microbiology Basic of microbial existence: history of microbiology. single cell protein (SCP). T. 2. Chand & Co.. R. Oxford and IBH Publishing Co. 2. Translation in Prokaryotes and Eukaryotes. wine). Meerut. Multiplication of bacteria. Weiner AM. biopesticide.House. trp Text Books 1. harvest. Plant Biotechnology. Roberts JW. UNIT V Gene Regulation Gene regulation-operon concept-gal. Nutrient requirements of bacteria. Rastogi Publications. 1987 3. food industries etc.1999 09BT249 INTRODUCTORY MICROBIAL BIOTECHNOLOGY Credits: 3:0:0 Objective To develop skills of the students in the field of microbial biotechnology and its applications. Preservation and maintenance of microbes. vitamins (B12). food industries and Agriculture Production. Molecular Biology of the Gene. antibiotics. organic acids (acetic acid. Genome.

Karunya University  Page 674  . 09BT250 APPLIED BIOTECHNOLOGY Credits: 3:0:0 Objective To develop skills of the students in the field of applications in biotechnology. P. 1995. the organization of cells into tissues and organs. bioethanol and biodiesel. 3 Das H K. animal feed. New York. Himalaya publishing house. Text Book of Biotechnology. UNIT 1: Cells. cloning. and Nikaido. W.Bioreactors for waste water treatmentActivated sludge process. Outcome At the end of the course. H. DNA. and Organs Introduction to the basic principles of cell structure. Unit-V: Biomass energy Energy sources – A general account. biofertilizers.. Principles of Fermentation Technology.definition and modes for utilization of biomass.Composting. A. New Delhi. Biotechnology: A text book of industrial microbiology. Microbial Biotechnology. Bombay 1995. 4 Microbial biotechnology: Glazer. Text Books: 1 Pelczar MJ. biomass. India. P. Wiley Dreamtech Pvt Ltd. vernalisation. biomedical waste treatment. Aditya Books (P) Ltd. 5 Jogdand SN. Unit – IV: Biotechnology for solid waste management Solid waste treatment . bioreactors. 2007.School of Biotechnology and Health Sciences Department of Biotechnology (BT) Unit – III: Bioremediation Xenobiotics-microbial mechanism. Fluidized bed reactors (FBR) Use of immobilized enzymes and microbial cells. Rotating biological contactors (RBC). . oil spill remediation. New Delhi. UNIT 2: Cell and Molecular Biology Introduction to the basic principles of biochemistry and molecular biology.. Tissue. Aavishkar Publisher 2004. 2nd Edition. Biological filters. biogas production. mushroom cultivation. microbial mining. Panima publishing corporation. 2001.J. the students would have learnt about the tissue culture.F.C. A. Whitaker. useful features of biofuel. Environmental Biotechnology. Tata McGraw Hill Edition. and Hall. This will serve as an effective course to understand the gene expression and human biology.H. 2 Trivedi. Microbiology. 2 Wolf Cruger and Anneliese Cruger. Vermicomposting. 2005. Chan ECS and Krein NR. genetic code. 2003 Reference Books: 1 Stanbury. .N. S. 1st Edition. Freeman &Company. photo periodism etc.

Biofertilizers..C. Chand & Co. Trevan. 2002.Trivedi. history and scope. Biotechnology. Elements of Biotechnology.D. the students would have learnt about principles of spectroscopy. 2nd Edition. Transgenics.. nephelometry & chromatography and all biotechniques Unit I Buffer Solutions (13) Definitions. Tata Mc Graw Hill Publishing Co. Outcome At the end of this course.A Brown .media. BIOS Scientific Publishers Ltd. Meerut. preparations. UNIT 5: Human biology Nervous system. brain. S. Freeman and Company. W. passive and adaptive immunity. determination of pH using H2 electrode and glass electrode. 1st Edition. calibration of instrumental methods Karunya University  Page 675  . UNIT 4: Plant Biology Biotechnology definition. Isolation and cultivation of economically important microbes.. callus formation and protoplast fusion.Production of primary and secondary metabolites. Genome. Micropropogation. New Delhi. Overview of Immune system -active.H. J. 2. GMOs. Microbial Biotechnology. Tissue culture. parts of brain and their functions.C. 2004 2. derivation of Henderson-Hasselbalch equation and its application.K. Dubey. Lamarckism Lysenrko. Immunology. M.. Single cell protein. circulatory system. Stem cells and applications. 1993 09BT251 INSTRUMENTAL METHODS OF ANALYSIS Credit:3:0:0 Objective To develop skills of students in instrumentation and biological techniques.School of Biotechnology and Health Sciences Department of Biotechnology (BT) UNIT 3: Genetics and Genetic engineering Principles of Mendelian inheritance and chromosomal basis of heredity. 3.. Gupta P. Text Book of Biotechnology. Aavishkar Publisher(2004) 4. buffering systems of blood. The Biological Principles. P. T. Rastogi Publications. Kuby.. New York1994. R.. heart and its function. 2004 Reference Books: 1. Organs and cells involved in immune responses Text books: 1. gene therapy.

gel filtration. types . VI Edition.thermo gravimetric. Flourimeter. 2000. Isoelectric focussing.Lambert’s law. Instrumental Methods and Analysis. Skoog. Flame Photometer and Spectrophotometer (13) Beer . GM counter. Flourimeter.principles. History of Nanotechnology. 2 Braun. radioactive techniques-RIA. Length scales . Instrumental methods of Chemical Analysis. affinity. Principle. Flame photometer and Spectrophotometer: types– UV – visible – IR – Raman spectroscopy.paper. McGraw Hill Book Company. 1989. adsorption. McGraw Hill. thin layer. construction and working of Differential Thermal Analysis (DTA) and Differential Scanning Calorimeter (DSC) Total: 65 Hours Text Books 1 Willard and Merrit. Introduction to Chemical Analysis. Unit III Chromatography and Electrophoresis (13) Chromatography.Basic theory. 2 Instrumental Methods of Analysis. Scope and Overview. clinical application and ethical issues of nanobiotechnology UNIT 1 Introduction.School of Biotechnology and Health Sciences Department of Biotechnology (BT) Unit II Colorimeter. D. ion-exchange. radioactive decay and their types. 2002. Applications in Medicine & Diagnosis. Reference Books 1 Ewing GW. description and application of Colorimeter. 1987 BT252 NANOBIOTECHNOLOGY Credit: 3:0:0 Objective: The aim of the course is to provide basic knowledge on nanobiotechnology Outcome: At the end of the semester the students would have learnt about Nanoparticles. Future of Nanotechnology: Nano Technology Revolution. gas and HPLC. Unit V Thermo Analytical Techniques (13) Theory of thermal analysis. Electrophoresis – principles. types – disc. Importance of Nanoscale and Technology. H. CBS Publishers & Distributors. Scintillation counter. Unit IV Radio active techniques (13) Radioactive isotopes. Silicon based Technology Karunya University  Page 676  . immuno-electrophoresis.

Prentice Hall. 4. Ratner D – “Nanotechnology: A Gentle Introduction to the next Big idea”. Third edition. Silica. Goodsell – “ Nanobiotechnology”. Reference Books 1. Karunya University  Page 677  . Nano-Biodevices and Systems. UNIT 3 Application: Nanomedicine. Cobalt. UNIT 5 Ethical Issues in Nanotechnology: Introduction. Types of Nanoparticles. H. John Willey & Sons Ltd. Nanomedicine. Artificial neurons. CNR Rao – “ The chemistry of Nanomaterials: Synthesis. Metal Oxides. 2004. Social and Ethical Issues. Properties & Application” Springer 2006 2. Nanocrystals in biological detection. High throughput DNA sequencing with nanocarbon tubules. Nanobiosensor and Nanofludics.MEMS/NEMS. Socioeconomic Challenges. Silver. 3. Early diagnosis of cancer and about treatment methods to cure cancer. Real-time nanosensors... 2003. 2. Nano wires and Nanotubes. Fabrication of Novel Biomaterials UNIT 4 Nanobiotechnology: Clinical applications of nanodevices.“Nanoscale Technology in Bilogical systems”. Future Socio-economic Challenges Text Books 1.School of Biotechnology and Health Sciences Department of Biotechnology (BT) UNIT 2 Nano Particles :Introduction. Ralph S. Nanosurgical devices. Ethical Issues in Nanotechnology: With Especial Reference to Nanomedicine. Nano Particles. Synthetic retinyl chips based on bacteriorhodopsins. Nalwa – “Encyclopedia of Nanoscience & Nanotechnology” McGraw Hill. 2005 BT253 CANCER BIOLOGY Credit : 3:0:0 Objective: The aim of the course is to provide basic knowledge on cancer biology Outcome: At the end of the semester the students would have learnt about Carcinogenisis. Greco. S. Silicon. 2004. Pure Metal. Economic Impacts. . Toxic effects of Nanomaterials. Electrochemical DNA sensors and Integrated Nanoliter systems. Gold. Iron oxide. Significance of Nanoparticles Nanofabrications. 2008. Applications. Alumina. Ratner M. Zinc oxide. Viola Vogel –“ Nanomedicine & Nanotechnology”.Applications in cancer biology. Characterization of Nanoparticles. John Willey & Sons Ltd. Techniques to Synthesize Nanoparticles. CRC Press. Titania.

tumor markers. The Molecular Biology of Cancer. 2001.J. IRLl Press. Robert G. Maly B. Barry Pierce. UNIT IV : Principles Of Cancer Metastasis Clinical significances of invasion. metabolism of carcinogenesis. chemotherapy. “Virology A Practical Approach”. 2. UNIT III : Principles Of Molecular Cell Biology Of Cancer Signal targets and cancer. Perantoni. UNIT II : Principles Of Carcinogenesis Theory of carcinogenesis. heterogeneity of metastatic phenotype. retroviruses and oncogenes.W. 2. advances in cancer detection. detection of oncogenes. Ralph E. 2002. x-ray radiation-mechanisms of radiation carcinogenesis. proteinases and tumour cell invasion. Gene therapy. Karunya University  Page 678  . G. detection of cancers.School of Biotechnology and Health Sciences Department of Biotechnology (BT) UNIT I : Fundamentals Of Cancer Biology Different forms of cancers. prediction of aggressiveness of cancer. radiation therapy. Use of signal targets towards therapy of cancer. UNIT V :New Molecules For Cancer Therapy Different forms of therapy. Oxford. Molecular Biology of Cancer. Text Books 1. 2006. Parchment. molecular tools for early diagnosis of cancer. The Biological Basis of Cancer. Chemical carcinogenesis. Reference Books: 1. three step theory of invasion. Alan O. identification of oncogenes. diet and cancer. McKinnell. Macdonald F and Ford CHJ. principles of physical carcinogenesis. New York 2003. Cancer screening and early detection. Oncogenes. metastatic cascade. Oncogenes/proto oncogene activity. 09BT254 BASIC RESEARCH METHODOLOGY Credit: 2:0:0 Objective To impart the knowledge about the method by which the research has to be carried out. basement membrane disruption. Stella Pelengaris and Michael Khan. activation of kinases. Detection using biochemical assays. Bios Scientific Publishers. Cambridge University Press. Blackwell Publishers.

Probability analysis – Testing of significance – Goodness of fit (X2 test) – Student’s ‘t’ test Analysis of Variance. Biotechnology’s impact on society. Review monographs. London. 3. 2001. Al. patent. recombinant material safety. Research/Experimental design. London. Methods and techniques. Quantification of cells by trypan blue exclusion dye 4. Raymond – Alain Thie’ tart. et. the Genetic revolution: Ethical issues. Jerrod H.R. IPR. Graphs. Ranjit Kumar. Reference Books: 1. Sage Publications. UNIT IV: Biostatistics Definition and scope. UNIT II: Laboratory safety Measures Laboratory safety. 2. Manuscript/research article preparation. Ltd. C. Donald H. Singapore. Sage publications. New Delhi. defining the research problem. UNIT III Experimental Design Essential steps in research. London. Thomson Asia Pvt. McBurney. Diagrams). Literature citation. Bibliography and Reference. Significance of research. Primary cell culture from chick embryo fibroblast 6. Press. Prentice Hall International. Inc. Cryopreservation Karunya University  Page 679  . Publishers. Text Books: 1. New Age Internation (P) Ltd. The future of bioethical biotechnology. Kothari.. 2002 2. Literature collection. Zar (1999) Biostatistical analysis by. II edition (2004) Research methodology. Types of biological data – Collection and presentation of data (Table. Isolation of macrophages and cultivation of macrophages 5. Format of thesis and dissertation. Packing and sterilization of glass and plastic wares for tissue culture 2. research methods. New Delhi. Genetic engineering and biowarfare. Preparation of reagents and media for cell tissue culture 3. Research methodology. 09BT255 ANIMAL AND PLANT TISSUE CULTURE LAB Credits 0:0:2 List of Experiments 1. Doing Management research. Standard operation protocol. 2006.School of Biotechnology and Health Sciences Department of Biotechnology (BT) Outcome The students will be familiar with experimental design data collection and Biostatics. bio safety. UNIT I Introduction to Ethical Issues Safety and risk of bioethical concerns. UNIT V: Manuscript/Thesis Formulation Research report: components.

Inspection and Checklists. Karunya University  Page 680  . Callus and Sub culturing from explants 11. 3. Safety and Health training. Unit – 4 Electrical Hazards and Hazards in Construction Industry Overload and Short circuit protection. Unit. I. John R. injuries.School of Biotechnology and Health Sciences Department of Biotechnology (BT) 7. burns. Rescue and Transport of Casualty. Masters.W. Animal Cell Culture Techniques. accidents caused by electricity. Rooting media preparation. Advantages of good housekeeping Unit – 3 First aid and Safety equipments First aid . Freshney. Safety and good housekeeping. Cleaning methods. Use of colour as an aid of housekeeping. Sterilization and inoculation of explants 10. Genetic transformation by using Plasmid References 1. Earthing standards. Preparation and Sterilization of MS media 9. Disposal of scrap and other trade wastes. Protection against voltage fluctuations. Safety committee and Function Unit. 09BT256 INDUSTRIAL SAFETY Credits 3:0:0 Objective: To provide basic knowledge of hazards and therefore the necessary safety measure in industrial planning and function. Ergonomics. Electrical equipment in hazardous atmosphere.2006. Micro grafting and hardening of explants 13.fracture and dislocation. Outcome: At the end of the semester student would have learnt about occupational health. Wiley-Liss. industrial hygiene. sterilization and rooting of explants 12. accidental prevention techniques to the students and risk assessment and management. Clynes. Effects of shock on human body Hazards from Borrowed neutrals. Culture of Animal Cells. 2. role of safety department. 5th Edition. Isolation and culture of T lymphocytes from Thymus 8. Respiratory problems.2 Plant design and Housekeeping Plant layout. Promoting Safety. 2006. Air conditioning. Spillage prevention. R. 3rd Edition. design. M.1 Safety and Health Management Occupational Health Hazards. Oxford. Ventilation. Personal Protective Equipments and devices. Animal Cell Culture: Practical Approach. bleeding. Importance of Industrial safety. Springer. 2ndt Edition.2005.

New Delhi (2001) 09BT257 RENEWABLE ENERGY SYSTEM Credits 3:0:0 Objective: To provide Engineers with inter-disciplinary skills and an understanding of energy systems because they will be in demand in energy supply companies. Industrial Safety . Karunya University  Page 681  . Industrial Visit: Report – Mandatory Text Book: 1.Child Labour and Women Employee Act. Health and Environment Management Systems.School of Biotechnology and Health Sciences Department of Biotechnology (BT) Unit – 5 Legislative measures in industrial safety Factories Act. Unit 1 INTRODUCTION World energy use-reserves of energy resources-energy cycle of the earth-environmental aspects of energy utilisation-renewable energy resources and their importance. The factories rules and regulations. AITBS Publishers . energy intensive manufacturing and process industries.Environmental Aspects-Potential impacts of harnessing the different renewable energy resources. GEO THERMAL AND HYDRO ENERGY SOURCES Introduction-basic theory-types of turbines-applications-Geothermal EnergyIntroduction-geothermal resource types-resource base-applications for heating and electricity generation-Hydropower-introduction-basic conceptssite selection-types of turbines-small scale hydropower. K. R. Introduction-Open and Closed OTEC cycles-biophotolysis-Ocean Currents-Salinity Gradient Devices. energy consulting and financing companies. Outcome The background provided in this course will equip students with the tools and techniques required to analyze and improve conventional energy systems and design the sustainable energy systems of the future. Employees State Insurance Act. Grimaldi and Simonds . New Delhi (2006) Reference Book: 1. 1948. 1948. Unit -3 WIND. Safety Management.radiation at ground level-collectors-solar cells-applications of solar energy-Biomass Energy-Introduction-Biomass Conversion-Biogas Production-Ethanol Production-Pyrolysis and Gasification-Direct Combustion-Applications. Unit -4 TIDAL ENERGY OTHER RENEWABLE ENERGY SOURCES Introduction-origin of tides-power generation schemes-Wave Energy-Introduction-basic theory-wave power Devices. Rao . energy equipment manufacturers. Unit – 2 SOLAR ENERGY Introduction -extraterrestrial solar radiation . Khanna publishers . Jain and Sunil S. Workman’s Compensation Act. 1943.

School of Biotechnology and Health Sciences

Department of Biotechnology (BT)

Unit-5 ENERGY AUDITING Energy auditing: short term, medium term, long term schemes, energy conversion, energy index, energy cost, representation of energy consumption, Sankey diagram, energy auditing. Text Books: 1. W.R.Murphy, G.Mc.Kay- Energy Management, 1st edition – Butterwolfer &Co.Ltd. (2001) 2.Conventional & non conventional energy resources by G.K.Roy Tata McGraw-Hill (2003) References: 1. A.Duffie and W.A.Beckmann, Solar Engineering of Thermal Processes-John Wiley (1980) 2. T.N.Veziroglu, Alternative Energy Sources, Vol 5 and 6, McGraw-Hill (1978) 3. Conventional energy technology, Fuel and chemical energy by Tata McGraw- Hill book Co.Ltd. (1987) Websites: 1. http ://www.solstice.crest.orgl ,2. http ://www.res-.ltd-com,3. http ://www.mnes.mic.in 4. http ://www.ireada.org,5. http://sundancepower.com

09BT258 BIOREMEDIATION FOR INDUSTRIAL SECTORS Credits: 3:0:0 Objective: This course is intended to provide beginning engineering students with a clear overview of the field of biotechnology in various industrial sectors. Outcome At the end of the semester the student will know about the various products and industries of biotechnology Unit-1 Pulp & Paper Industry Problems related with pulp and paper industry, Environmental impact of pulp and paper mills, Treatment of pulp and paper mill pollutants, Ex-situ and In-situ bioremediation, Bioconversion and bioutilisation of effluent for products. Unit-2 Distillery Industry Environmental impact and treatment of distillery industry effluents-Primary, secondary & alternative treatments, wetland method, Prospects of Bioutilisation for products-foods, feeds, Soil and land applications, Fertilizers, Production of fuels. Unit-3 Leather Tanning Industry Environmental impact of tannery effluents-hazards and prevention, Treatment of tannery effluents-chemical & biological methods, Advanced methods-phytoremediation, Bioreactors, Microbial heavy metal accumulation.

Karunya University 

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Department of Biotechnology (BT)

Unit- 4 Petroleum industry Crude oil desalting, Petroleum wastes and their impact on environment, Treatment of petroleum waste, Biodegradation and bioremediation, Microbial recovery and up gradation, Biological treatment of dyes. Unit- 5 Food and Beverages Industry Prospects of utilization of food and beverages wastes-biochemical modification, enzymatic treatment, Treatment of waste water, solid waste and contaminated soil, Industry specific bioremediation, Text Book: 1) Indu Shekhar Thakur, Industrial Biotechnology –Problems and Remedies, 2006, I.K. International Pvt. Ltd, New Delhi. References: 1) Pradipta Kumar Mohapatra, Textbook of Environmental Biotechnology, 2006, I.K. International Pvt. Ltd, New Delhi. 2) A. H. Patel, Industrial Microbiology, 2007, Macmillan India Ltd, New Delhi. 3)Dunmock N.J And Primrose S.B., “Introduction to Modern Virology”, Blackwell Scientific Publications, Oxford, 2002. 4)An Introduction to Cellular and Molecular Biology of Cancer, J Oxford Medical Publications, 2003. 09BT301 CELL BIOLOGY AND ADVANCED BIOCHEMISTRY Credit: 4:0:0 Objective To enable the students to understand the various metabolic pathway and cell signalling

Outcome The students will be familiar in transport mechanism and various metabolic pathways and their significance UNIT I Structure, Function and Transport Mechanisms of the Cell (13) Structure and function of Eukaryotic and prokaryotic cells- its membrane- Micelles- different types of proteins & extra cellular matrix. Transport in eukaryotic cells-Passive & active transport, trans-membrane potential coupled ATP generation, endocytosis and exocytosis. Transport into prokaryotic cells-group transportation. Entry of virus and toxins into cells. UNIT II Cell-Cell Signalling and Apoptosis (13) Receptors - endocrine models of action- signal amplification. Different models of signal transduction-signalling by protein kinases-serine/threonine kinase –tyrosine kinase-MAP kinases. Cell cycle –it’s control molecules & mechanism-apoptosis-oncogenes.

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School of Biotechnology and Health Sciences

Department of Biotechnology (BT)

UNIT III Introduction to Biomolecules (13) Biomolecules: Occurrence, classification, structure, properties and functions of carbohydrates, proteins, lipids and vitamins. stabilization of proteins and nucleic acids. Structural and functional relationships in complex carbohydrates, proteins and nucleic acids. UNIT IV Enzymes (13) Enzymes-action, specificity, classification, structure, properties and function of enzymes. Enzyme kinetics: Michales- Menton equation, enzyme inhibition, kinetics of competitive, non – competitive and mixed inhibitors, allosterism, application of enzymes. UNIT V Metabolic Pathways and Bioenergetics (13) Energy yielding biochemical pathways: Biodegradation of Leu, Thr, Met, Lys, Tyr, Phe, Trp, Glutamate, Arg and Cys, fatty acids, cholesterol, purines and pyrimidines. Glycolysis, TCA cycle, pentose phosphate pathway. Bioenergetics: Redox biochemistry, energy rich compounds, respiratory chain, oxidative phosphorylation and triose phosphate cycle. Total: 65 Hours Text Books 1. Lehninger, A. L., Nelson, D. L. and Cox, M. M. Principles of Biochemistry Third Edition (Freeman Publishers), New York. 2000. 2. Darnell J, Lodish H, Baltimore D, Molecular Cell Biology, W.H.Freeman Fourth edition 2002. Reference Books 1. Harper’s Biochemistry – Robert K., Md Murray,Daryl K. Granner,Peter A. Mayes, Victor W. Rodwell,Appleton and Lange, Robert K. Murray 2000. 2. Donald Voet and Judith G. Voet .Biochemistry – Volume 1 Biomolecules, Mechanisms of Enzyme Action, & Metabolism; 2004. 3. A Text Book of Biotechnology by B. N. Prasad Budha Academic Publishers Pv.Ltd.G. P.O.Box 20195 Kathmandu,Nepal 2003.

09BT302 CELL BIOLOGY AND ADVANCED BIOCHEMISTRY LAB Credit: 0:0:2 List of Experiments: 1. Estimation of Protein by Lowry Method 2. Estimation of Total Carbohydrates by Anthrone Method 3. Estimation of Aminoacid by Ninhydrin Method 4. Estimation of Cholesterol by Zak’s Method 5. Estimation of Ascorbic Acid by volumetric Method 6. Differentiation of blood cells using Giemsa staining 7. Estimation of DNA 8. Study of phase contrast Microscope 9. Estimation of Chlorophylls 10. Staining for various stages of mitosis in Allium cepa (onion) 09BT303 BIOCHEMICAL ENGINEERING Credit: 4:0:0 Karunya University  Page 684 

School of Biotechnology and Health Sciences

Department of Biotechnology (BT)

Objective To acquire the knowledge about the various biochemical reactions and the ways to alter the biochemical pathways and about design and operation of bioreactors. Outcome At the end of the course the student will be familiar in fermentation process and metabolic stoichiometry and the way to enhance the product of interest UNIT I Media Design and Media Optimization Methods: (13) Criteria for good medium, medium requirements for Industrial fermentation processes, carbon, nitrogen, minerals, vitamins and other complex nutrients, oxygen requirements, medium formulation of optimal growth and product formation, examples of simple and complex media, design of various commercial media for industrial fermentations – medium optimization methods: Plakett Burman Design, Response Surface Optimization and Simplex Optimization methods. UNIT II Sterilization for Fermentation processes (13) Thermal death kinetics of microorganisms; Batch and continuous Heat- Sterilization of Liquid Media; Sterilization equipment – batch and continuous - air sterilization- sterilization by radiation and chemical sterilization-Filter Sterilization of liquid Media –Design of Depth filters UNIT III Metabolic Stoichiometry and Bioenergetics (13) Thermodynamics efficiency of gowth, cell growth and product formation; metabolic heat generation. Stoichiometry of Cell growth and product formation - elemental balances, available – electron balances, degrees of reduction of substrate and biomass; yield coefficients of biomass and product formation; maintenance coefficients; oxygen consumption and heat evolution in aerobic cultures. UNIT IV Transport phenomena in bioreactors (13) Mass transfer in heterogeneous biochemical reaction system: Oxygen transfer in submerged fermentation processes; oxygen uptake rates and determination of oxygen transfer coefficients (kla); role of aeration and agitation in oxygen transfer. Heat transfer processes in biological system. UNIT V Process design and operation of Bioreactors (13) Operational modes of reactors – batch, continuous, fed batch, repetitive batch, recycle and continuous cultivation; novel bioreactors: Stirred tank, air lift and loop reactor, packed – bed and hollow – fibre membrane bioreactors; reactors for waste – treatment processes; scale- up criteria for bioreactors. Total:65 hours Text Books: 1 Shuler and Kargi, Bioprocess engineering, Prentice Hall, Second Indian Reprint 2002. 2 Peter F. Stanbury, Stephen J. Hall & A. Whitaker, Principles of Fermentation Technology, Second Edition, Butterworth – Heinemann An Imprint of Elsevier India Pvt. Ltd., 1999 Reference Books: 1 Atkinson, B. & Mavituna . F., Biochemical Engineering and Biotechnology Handbook, McGraw Hill (2nd Edition) 1993. Karunya University  Page 685 

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2

Harvey W. Blanch and Duoglas S. Clark, Biochemical Engineering, Marcel Dekker Inc.1997 3 Bailey J.E. and Ollis, D.F. Biochemical Engineering Fundamentals, McGraw Hill, 1986. 4 Bioprocess Engineering Principles – Pauline M Doran, Academic Press, 1995

09BT304 CHEMICAL AND BIOCHEMICAL ENGINEERING LAB Credit: 0:0:2 List of Experiments: 1 Over all heat transfer coefficient in Heat Exchangers 2 Darcy’s Friction Factor 3 Pressure drop in fluidized bed column / Packed Bed column 4 Flow measurement through orifice meter/ Venturi meter 5 Minor losses due to sudden expansion, contraction and elbow 6 Screening analysis 7 Citric Acid Production by Solid State Fermentation 8 Enzyme Kinetics 9 Media Optimization by Plackett burman Design 10 Production of enzymes 11 Production of Amino acids 12 Comparitive study on rate of product formation using Immobilized enzyme & Free enzyme. 09BT305 ADVANCES IN GENETIC ENGINEERING Credit: 4:0:0 Objectives : The student will be familiar in the demonstrate the basic mechanisms of gene expression, methods of cloning , basics of sequencing and blotting techniques., various techniques in genetic engineering and the applications of genetic engineering with caution on their hazards Outcome : At the end of the semester the students would have learnt about the basic tools of genetic engineering, the steps in gene expression, the use of and the essence of genetic regulation control mechanisms in both prokaryotes and eukaryotes, Isolate DNA using agarose gel electrophoresis, Perform DNA probing, Perform DNA recombination and mapping analysis. UNIT- I Molecular Biology (15) Prokaryotic and Eukaryotic gene expression -Transcription and Translation, control and regulatory elements , Eukaryotic gene expression in E.coli – fused genes, unfused genes, Gene expression in transgenic animals and plants UNIT- II Gene Cloning (13)

Introduction to Cloning-methods .- how to isolate a gene – plasmid , marker techniques, Gene library – construction and applications of genomic library, cDNA library, phage lambda versus Karunya University  Page 686 

School of Biotechnology and Health Sciences

Department of Biotechnology (BT)

cosmids for gene libraries, Mapping the DNA- restriction mapping DNA foot printing, chromosome walking, chromosome jumping. UNIT- III Analysis of Nucleic Acids and Proteins (12)

DNA sequencing – MAXAM and GILBERT chemical method, SANGER and COULSON Enzymatic chain termination method – the primer, the template, the dideoxynucleotide termination and deoxynucleotides, the polymerase etc. , Messing’s shotgun method, using computers for sequencing .Blotting techniques- Southern , Northern and Western. UNIT- IV Techniques in Genetic Engineering (12) DNA Engineering Techniques – oligonucleotide synthesis, linkers, adapters and connectors, Mutagenesis, Site Directed Mutagenesis; transposon mutagenesis, transposon tagging., Gene targeting, Repetitive DNA Sequences - Variable Number of Tandem Repeats (VNTRs)/ Minisatellite sequences, Short Tandem Repeats (STRs)/ Microsatellite sequences, DNA finger printing, Hybridization based DNA fingerprinting,, RFLP, RAPD- PCR-based DNA fingerprinting, UNIT- V Applications and Hazards of Genetic Engineering (13) Applications of DNA fingerprinting and Genetic Engineering – -Criminal investigation, personal identification, Immigration, Paternity disputes , Exploitation of genetic engineering, Transgenic plants and Transgenic animals Genetically Engineered Microbes, . stem cells and their applications, gene banks, Hazards and Impact of Genetic Engineering on society. Total:65 hours Text books : 1. Genetic Engineering Principles and Practice by Sandhya Mitra Macmillan Publ.2008. 2. Gene Biotechnology, by S.N. Jogdand Himalaya Publishing House 2003 Reference books: 1. Text book of Biotechnology edited by H.K. Das 2nd edn. Wiley India (P) Ltd. 2006 2. Principles & Techniques of Biochemistry and Molecular Biology Wilson & Walker 2006. 3. Molecular Biology and Biotechnology Walker and Rapley 4th edn. 2nd Indian reprint Panima Publishing Corpn. 2003 4. Biotechnological Innovations In Crop Improvement, Biotol Book series. ButteworthHeinemann- An imprint of Elsevier 2004. 5.Text book of Biotechnology R. C. Dubey. 2nd edn. S. Chand & Co. New Delhi. Publ. Jasper Rees. 2005 09BT306 GENETIC ENGINEERING LAB Credit: 0:0:2 List of Experiments: Isolation of genomic dna from plant tissue Isolation of genomic dna from animal tissue Quantitative and qualitative analysis of isolated genomic dna using spectrophotometer Elution technique for separated dna from agarose gel Extraction of proteins from plant or animal tissue and confirmation with qualitative tests Separation and identification of proteins by sds-page using coomassie brilliant blue stain Isolation of plasmid dna from e.coli Karunya University  Page 687 

1. 2. 3. 4. 5. 6. 7.

Transformation competent cell preparation 11.mass transfer in biological systems Unit. Unit. Prey.Application of Artificial Intelligence. basic configuration of fermentor and ancillaries.M kinetics.IV Bacterial growth kinetics (13) Simple unstructured and structured kinetic models for growth of bacterial and filamentous organisms. Total:65 hours Textbooks: Karunya University  Page 688  . Ligation of fragmented puc19 plasmid dna and testing with agarose gel electrophoresis 10. minimizing mass transfer effects.dynamics of multiple interacting microbial species. Unit. Case studies on bioreactor control problems. main parameters to be monitored and controlled in fermentation processes. Role of endogeneous metabolism in substrate utilization and growth Unit. mass transfer correlations. enzyme deactivation kinetics.M. internal mass transfer in heterogenous reactions. heterogenous reactions in bioprocessing. general requirements of fermentation processes.Predator interaction oscillations.I Overview of Fermentation Processes and Scale-up Criteria (13) Overview of fermentation industry. growth associated product formation kinetics-Monod. Fluid flow and mixing. Restriction enzyme digestion of puc19 plasmid dna and testing with agarose gel electrophoresis 9.School of Biotechnology and Health Sciences Department of Biotechnology (BT) 8. Thiele modules . They will be able to know the various factors that will the kinetics of the microorganism Unit. Polymerase chain reaction (pcr) for the given standard dna using pcr kit and verifying with agarose gel electrophoresis 09BT307 ADVANCED BIOPROCESS ENGINEERING Credit: 4:0:0 Objective To know about the various Bioprocess and the ways to enhance the production of industrially important bioproducts Outcome At the end of the semester the students will be able to analyze various bioprocess and it control. Fuzzy logic and neural networks in bioprocess control.solid – liquid mass transfer correlations. Transformation of recombinant plasmid dna into competent cells 12.power requirements for agitation. Leude king piret modelsformulation of models for various biochemical processing systems in steady and unsteady state conditions. Concepts of process control. mass and energy balances for steady state and unsteady state biochemical systems. Scale-up criteria for mixing.II Data Analysis in bioprocess (13) Collection and analysis of data on bioprocess engineering.V Process Control (13) Online data analysis of physico chemical parameter measurements for biochemical processes.concentration gradients and reaction rates in solid catalysts.III Reaction Kinetics in bioprocess (13) Reaction kinetics for biological systems.

Ammonium Sulphate precipitation 8. the students would have learnt about various methods synthesis of primary and secondary metabolites and bioconversion." Bio-process Engineering". Batch Sedimentation 5. 5 Roger Harrison et al. Reference Books: Books 1 Biochemical Engineering by James M. 3 Bioreactor Design and Product Yield . Biotol Series. 4 Colin Ratledge. Estimation of Biomass 09BT309 METABOLIC REGULATIONS AND ENGINEERING Credit: 4:0:0 Objective To develop skills of the students in the area of metabolic engineering to alter the metabolic pathway to enhance the product yield. 6 Bioprocess Engineering Principles – Pauline M Doran. 2nd Edition.coli 10. 2nd Edition.L. Immobilization of Enzyme-α amylase 11. Outcome At the end of the course. 1995 09BT308 BIOPROCESS ENGINEERING & DOWNSTREAM PROCESSING LAB Credit: 0:0:2 List of Experiments: 1. 1993. Estimation of MM parameters 9.H. Production of wine 14.School of Biotechnology and Health Sciences Department of Biotechnology (BT) 1 Biochemical Engineering Fundamentals – 2nd edition Bailey and Ollis. 2 M. Liquid-Liquid extraction 6. Academic Press. Mcgraw Hill 1986. 2001. 2. Butterworth – Heinemann. Centrifugation 4. Prentice Hall 1992 2 Bioreactors in Biotechnology – A practical approach by A. 2002. Karunya University  Page 689  .Oxford University Press. Effect of temperature on enzyme activity 12. Effect of substrate concentration on growth of E. Prentice Hall of India. Determination of KL a by sodium sulphite oxidation method 3. Batch Distillation 7.Scragg.New Delhi. Effect of pH on Enzyme activity 13. Bjorn Kristiansen.Lee . Shuler and F. Demonstration of a Fermentor and its components. Basic Biotechnology. 2003. Cambridge University Press. Bioseparations Science and Engineering.. 1991. Kargi.

School of Biotechnology and Health Sciences

Department of Biotechnology (BT)

UNIT - I Overview of Primary and Secondary Metabolic Pathways (13) A brief outline of processes for the production of some commercially important primary metabolites such as amino acids and alcohols, various classes of secondary metabolites such as secondary metabolites: antibiotics: beta-lactams (penicillin, cephalosporin etc.), aminoglycosides (streptomycin etc.,) macrolides (erythromycin), vitamins and steroids, Industrial enzymes such as proteases, amylases, lipases, cellulases etc.,and enzyme inhibitors such as inhibitors of cholesterol synthesis. UNIT – II Metabolic Regulations (13) Operon concept-Regulation of gene expression in prokaryotes-Lac operon. Regulation of gene expression in eukaryotes- catabolite regulation. Regulation of RNA synthesis by amino acid. Feedback regulation, regulation in branched pathways- differential regulation by isoenzymes, concerted feedback regulation, cumulative feedback regulation. Permeability control: passive diffusion, active transport, group transportation. UNIT –III Metabolic Engineering of Primary Metabolites and enzymes (13) Primary metabolites: Alteration of feedback regulation, limiting accumulation of end products, feedback resistant mutants, alteration of permeability for metabolites. Enzymes: Strain selection, improving fermentation, recognising growth cycle peak, induction, feed- back repression, catabolite repression, mutants resistant to repression, gene dosage. UNIT – IV Metabolic Engineering of Secondary Metabolites (13) Producers of secondary metabolites, Precursor effects, trophophase, idiophase relationship, enzyme induction, feedback regulation, catabolite regulation by passing control of secondary metabolism. UNIT - V Metabolic Engineering in Bioconversions (13) Metabolic bioconversion pathways, Advantages of bioconversions, specificity, yields, factors important to bioconversion, regulation of enzyme synthesis, mutation, permeability, cometabolism, avoidance of product inhibition, mixed or sequential bioconversions, conversion of insoluble substances. Total:65 hours Text book 1. Peter F. Stanbury, Stephen J. Hall & A. Whitaker, Principles of Fermentation Technology, Second Edition, Butterworth – Heinemann An Imprint of Elsevier India Pvt. Ltd., 2005 Reference Books: 1. Wang D.I.C., Cooney C.L., Demain A.L., Dunnil.P., Humphery A.E., Lilly M.D., " Fermentation And Enzyme Technology ", John Wiley And Sons., 1980. 2. Zubay G., " Biochemistry ", Macmillan Publishers, 1989.

09BT310 ADVANCED IMMUNOTECHNOLOGY Credit: 4:0:0 Objective This course aims to develop the skills of the students in Immunotechnology, antigen antibody response, immunodiagonosis, immunopathology etc. Karunya University  Page 690 

School of Biotechnology and Health Sciences

Department of Biotechnology (BT)

Outcome At the end of the course would have learnt about the mechanisms by which a human body interacts with a pathogenic microbe & how it eliminates it. Students, also familiarize themselves with immunopathology and immunotherapy. UNIT I Antigens (13) Types of antigen, their structure, preparation of antigen for raising antibodies, peptide antigens preparation, handling of animals, adjuvants and their mode of action, identification of antigens by molecular techniques UNIT II Antibodies and Immunodiagnosis (13) Recombinant monoclonal and polyclonal antibodies – their production and characterization, Western Blot analysis, Immunoeletrophoresis, SDS-PAGE, Purification and synthesis of antigens; ELISA – Principle and application; Radio Immuno Assay (RIA) – Principles and applications; non isotopic methods of detection of antigens – enhanced chemiluminescence assay. UNIT III Techniques to assay Cell Mediated Immunity (13) Identification of lymphocytes and their subsets in blood, T-cell activation parameters, estimation of cytokines, macrophages activation, macrophage microbicidal assays, invitro experimentation; application of the above technology to understand the pathogenesis of infectious diseases and treatment; cytotoxicity assay; helper T- cell assay; cytotoxic T- cell assay. UNIT IV Immunopathology (13) Preparation of storage of tissues, identification of various cell types and antigens in tissues, isolation and characterization of cell types from inflammatory sites and infected tissues, functional studies on isolated cells, immunocytochemistry – immunofluorescence, immunoenzymatic and immunoferrtine techniques, immuno electron microscopy, ELISA and ELISPOT UNIIT V Molecular Immunology (13) Preparation of vaccines, application of recombinant DNA technology for the study of the immune system production anti idiotypic antibodies, catalytic antibodies, application of PCR technology to produce antibodies, immunotherapy with genetically engineered antibodies. Total: 65 hours Text Books: 1 Talwar G. P., and Gupta S.K., A Hand book of Practical and Clinical Immunology, Vol. 1 & 2, CBS Publications, 2004. 2 Weir D.M., Practical Immunology, Blackwell Publications, 2000. Reference Books: 1 Austin J.M. and Wood K.J., Principles of Cellular and Molecular Immunology, Oxford University Press, Oxford, 2003. 2 Frank C. Hay, Olwyn M. R. Westwood, Paul N. Nelson, Hudson Practical Immunology, Blackwell Publishers; 1st edition, 2002. 3 Ivan Roitt, Essential Immunology, 10th Edn. Blackwell Scientific Publication, 2002. 4 Weir DM and Stewart, J, Immunology, 10th Edn. Churchill Livingston, New York. 2000. Karunya University  Page 691 

School of Biotechnology and Health Sciences

Department of Biotechnology (BT)

09BT311 IMMUNOTECHNOLOGY LAB Credit: 0:0:2 List of Experiments: 1. Blood Grouping and Rh typing 2. Preparation of Plasma and Serum 3. Single Radial Immunodiffusion 4. Double Immunodiffusion – Ouchterlony Method 5. Immunoelectrophoresis 6. Counter Current Immunoelectrophoresis 7. Rocket Immunoelectrophoresis – Laurell method 8. Immunodiagnosis of Typhoid fever – Widal Test 9. Enzyme Linked Immunosorbent Assay 10. Preparation of Antigen and Various Routes of Immunization 11. Isolation of PBMC from blood and checking the viability using Trypan Blue assay 12. Quantitative precipitin assay 13. Serum Electrophoresis 09BT312 ADVANCED BIOPHARMACEUTICAL TECHNOLOGY Credit : 4:0:0 Objective To develop skills of students in Biopharmaceutical technology. Outcome At the end of this course, the students would have learnt about principles of pharmacokinetics, pharmacodynamics, drug delivery, analytical techniques and bioproducts. Unit- I Fundamentals Of Pharmacy Practice Pharmaceutical Biotechnology: An introduction, Origin & definition, Scope & Importance of Biotechnology; their applications, Microbes in Pharmaceutical industry, Methods of Gene transfer, Biotechnology production of Secondary Metabolites Unit- II Pharmacokinetics And Pharmacodynamics ADME properties- Mechanism of Drug Absorption, Distribution of drugs, Drug metabolism (Biotransformation of drugs), Excretion of drugs, Pharmacokinetics: Basic considerations. Mechanism of drug action. Unit- III Pharmaceutical Dosage And Drug Delivery System Materials & Formulations, Manufacture of Tablets, Capsules, Sustained Release dosage forms, Parenteral solutions, Oral liquids, Emulsions, Ointments, Suppositories, Aerosols. Drug delivery system – Formulation of proteins and peptides. Protein as drug delivery system. Controlled drug delivery system- transdermal. Novel drug delivery system- liposomes

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School of Biotechnology and Health Sciences

Department of Biotechnology (BT)

Unit IV Pharmaceutical Analytical Techniques UV-Visible spectroscopy, Flame emission spectroscopy and atomic absorption Spectroscopy, Fluorimetry, Infrared spectrophotometry, Nuclear magnetic resonance spectroscopy, Electron spin resonance spectroscopy, Mass spectroscopy, Chromatographic techniques, Electrophoresis, Radio immuno assay, X-ray diffraction methods. Unit V Biological Products Properties of biotechnology derived therapeutic products. Production of Human insulin, Interferons, somatotropin, human growth hormone, somatostatin, purification, characterisation & analysis- establishing safety & efficacy. Gene Therapy, vaccines, Monoclonal Antibody Based Pharmaceuticals, Recombinant Human Deoxyribonuclease Text Books 1 Pharmaceutical Biotechnology by S.S. Purohit, H.N. Kakrani and A.K. aluja. Jodhpur, Agrobios, 2003. 2 Biopharmaceutics and Pharmacokinetics-A Treatise by DM Brahmankar, Sunil B Jaiswal,2005. Reference books: 1. Gary Walsh “Pharmaceutical Biotechnology Concepts and Applications” by,2007 2. Pharmaceutical Dosage Forms and Drug Delivery Systems by Ansel, H., Allen, L., Popovich, N.. Williams & Wilkins. 1999. 3. Instrumental Methods of Chemical Analysis – B. K. Sharma - 2004. 4. Pharmaceutical Biotechnology, edited by Michael J. Groves, Second Edition,2006. 5. Willard and Merrit, Instrumental Methods and Analysis. VI Edition, CBS Publishers & Distributors; 1992 09BT313 DOWN STREAM PROCESSING IN BIOTECHNOLOGY Credit: 4:0:0 Objective To develop skills of the students in the area of Downstream processing with emphasis on purification of products. Outcome At the end of the course, the students would have learnt about various methods of separation and purification of bioproducts. Unit I Role of Downstream Processing In Biotechnology (13) Role and importance of downstream processing in biotechnological processes. Problems and requirements of bioproduct purification. Economics of downstream processing in Biotechnology, cost-cutting strategies, characteristics of biological mixtures, process design criteria for various classes of bioproducts (high volume, low value products and low volume, high value products), physico-chemical basis of bioseparation processes Unit I I Primary Separation and Recovery Processes Karunya University  (13) Page 693 

School of Biotechnology and Health Sciences

Department of Biotechnology (BT)

Cell disruption methods for intracellular products, removal of insolubles, biomass (and particulate debris) separation techniques, flocculation and sedimentation, centrifugation and filtration methods Unit III Enrichment Operations (13) Membrane based separations micro and ultra filtration theory, design and configuration of membrane separation equipment, applications, precipitation methods (with salts, organic solvents, and polymers, extractive separations, aqueous two-phase extraction, supercritical extraction) insitu product removal, integrated bioprocesing Unit IV Product Resolution/Fractionation (13)

Adsorptive chromatographic separation processes, electrophoretic separations (all electrophoresis techniques including capillary electrophoresis) hybrid separation technologies (membrane chromatography, electro chromatography etc) Unit V Product Polishing Gel Permeation Chromatography, dialysis, Crystallisation (13)

Text book 1. Bioseperations: Principles and Techniques, B. Sivasankar,Published by PHI Learning Pvt. Ltd., 2006 2. Bioseparation Technology,Mishra Neeraj,Publisher: CRC Press,2008 Reference Books: 1. Bioseparations Science and Engineering ,Day, Trevor G, and Harrison, Roger G, and Rudge, Scott R,Publisher: Oxford University Press, USA, 2002 3. Handbook of Bioseparations,Satinder Ahuja,Published by Academic Press, 2000 4. HPLC of Biological Macromolecules,Karen M. Gooding, Fred E. Regnier, Contributor Karen M. Gooding, Fred E. Regnier,Published by CRC Press, 2002 5. Isolation and Purification of Proteins,Rajni Hatti-Kaul, Bo Mattiasson,Published by CRC Press, 2003 6. A Century of Separation Science, Haleem J. Issaq,Published by CRC Press, 2002

BT314 ANALYTICAL BIOTECHNOLOGY LAB Credit 0:0:2 List of Experiments 1. 2. 3. 4. 5. 6. 7. 8. 9. TLC Paper Chromatography HPLC GC SDS PAGE Electrophoresis ELISA PCR Lyophilizer Agarose gel Electrophoresis Page 694 

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Department of Biotechnology (BT)

10. X-ray Diffraction instrument 11. Differential Scanning Colorimetry. 12. Nephlometry 09BT315 RESEARCH METHODOLOGY Credit: 4:0:0 Objective To impart the knowledge about the method by which the research has to be carried out. Outcome The students will be familiar with experimental design data collection and Biostatics. UNIT- I: Introduction to research (13) The hallmarks of scientific research – the building blocks of science in research – relevance to preliminary information, the research process for applied and basic research. Hypothesis development. Laboratory safety, bio safety, recombinant material safety. UNIT- II: Experimental designs (13) The laboratory and the field experiment – internal and external validity – factors affecting internal validity. Measurement of variables – scales and measurements of variables. Developing scales: rating scale and attitudinal scales. Validity testing of scales developed. Reliability concept in the scales being developed. Stability measures. In vitro, in vivo and clinical trial designs, rules and regulation for animal and human experiments. UNIT- III: Data collection methods (13) Interviewing questionnaires etc. secondary sources of data collection. Guidelines for questionnaire design – electronic questionnaire design and surveys. Special date sources: focus groups, static and dynamic panels. Review of the advantages and disadvantages of various data collection methods and when to use each. Sampling techniques. Probabilistic and non – probabilistic samples. Issues of precision and confidence in determining sample size. Hypothesis testing. Determination of optimal sample size. Data relevance to intellectual property rights (IPR), bookkeeping. UNIT- IV: Biostatistics (13) Definition and scope, Types of biological data – Collection and presentation of data (Table, Graphs, Diagrams). Measures of central tendency, Dispersion : Skewness and Kurtosis; Probability analysis – Testing of significance – Goodness of fit (X2 test) – Student’s – test – Simple & multiple regression – Correlation: Canonical Correlation – Correlation coefficient– ANOVA (one way and two way analysis). Factor analysis – Cluster analysis – Discrimnant analysis. Application of SPSS package. UNIT- V: The Research Report (13) The purpose of the written report – concept of audience – Basics of written reports. The integral parts of a report – the little of a report, the table of contents, the synopsis, the introductory section, method section, results section – discussion section – recommendations and implementation section and reference section. Total: 65 hours Karunya University  Page 695 

2000 09BT316 INSTRUMENTATION AND BIOTECHNIQUES Credit: 4:0:0 Objective To develop skills of students in instrumentation and biological techniques. G. Flowcytometry Unit-II Separation and Analysis (13) Principles of Chromatography: lon exchange. Unit-IV Cell Culture and Molecular Biological Techniques (13) Preparation of Culture media. Designs: Fluidized bed. Kothari. Sage publications. 2002 2. Immunoelectrophoresis. Autoradiography. CD. Uma Sekaran. Isoelectrofoccusing Unit-III Structural Elucidation and Analysis (13) Principles and applications of the following: Microscope – Flourescence.School of Biotechnology and Health Sciences Department of Biotechnology (BT) Text Books: 1. C. Research methodology. Raymond – Alain Thie’ tart. Unit-I Quantitative analysis (13) Introduction . New Delhi. Immunoblotting. 5. Immunotechniques – ELISA. Immobilization of enzyme and cells. Cooper and remela S. 1999.J. John Wiley and Sons Inc. London.W. NMR. Business Research Methods. Electrophoresis – Polyacrylamide. et. Pulsed field electrophoresis. New Delhi. Donald R. Wishva Prakashan. size exclusion. Modes of sterilization. Thomson Asia Pvt. Nucleic acid isolation – Karunya University  Page 696  . Electron (Scanning and Transmission) –Radio isotopic techniques-X ray diffraction. Characterization of chromosomes by various banding techniques. Research Methodology.Spectrophotometer – Principle and application of Spectrophotometer – Visible. Schindler. Culture of microbes. Ranjit Kumar. 3. UV.R. GLC: GCMS.. 1999. IR. 2001. Tata McGraw Hill publishing company limited. Doing Management research. New Delhi. 4. Plant and animal cell and Tissue. New York. Confocal. 1999. the students would have learnt about principles of spectroscopy. Packed bed. and Affinity column. Denaturing gradient gel electrophoresis. nephelometry & chromatography and all biotechniques. Singapore. Ticehurst and A. Veal. scintillation counter. Fed batch. 2000. Batch. Al. Ltd. GM counter. Business research methods. London. Atomic absorption spectrophotometer – fluorimetry. McBurney. DSC. Reference Books: 1. Agarose. HPLC and Gas chromatography. Sage Publications.. 2. Longman. Phase contrast. Bioprocess Engineering: Operation types: Continuous. Centrifugation high speed and ultra. Outcome At the end of this course. research methods. Donald H. Research Methods for Business. RIA.

the students would have learnt about kinetics of heterogeneous reactions. Willard and Merrit. effects of diffusion. Instrumental Methods and Analysis. Ltd. 2005 2. (2007) 2. Langmuir-Hinshelwood model.Zar Biostatistical analysis. (2002) 09BT317 ADVANCED REACTION ENGINEERING Credit : 4:0:0 Objective To develop skills of students in the area of reaction engineering. Jerrold H. Singapore. Whitaker. Rideal-Eiley mechanism. Butterworth – Heinemann An Imprint of Elsevier India Pvt. bioinformatics and drug discovery. Principles of Fermentation Technology. Unit III Internal Transport Processes in Porous Catalysts Inter pellet mass and heat transfer.School of Biotechnology and Health Sciences Department of Biotechnology (BT) Isolation of genomic DNA. Inc.. protein-structural and sequencial data base. Total: 65 hours Text Books 1. Second Edition.K and Parry-Smith D. analysis and design of heterogeneous reactors. Unit I Kinetics of Heterogeneous Reactions Catalytic reactions. Biological reactions in fermentation processes Unit II External Diffusion Effects in Heterogeneous Reactions Mass and heat transfer coefficients in packed beds. Stanbury. shrinking and unreacted core model.. Upper Saddle River. evaluation of effectiveness factor. modeling diffusion with and without reaction.J. Plasmid DNA. Karunya University  Page 697  . Sequence analysis-BLAST. FASTA: Sequence comparision. Stephen J. Gene and protein expression data. Hall & A. Molecular visualization tools study protein structure. CBS Publishers & Distributors. VI Edition. Introduction to Bioinformatics. quantitative treatment of external transport effects. building phylogenetic trees. mass and heat transfer with reaction.biochemical reaction system. RNA. PCR and. 5edn. Outcome At the end of this course. Prentice-Hall. T. Peter F. non-catalytic fluid-solid reactions. Attwood. Unit-V Bioinformatics (13) Biological data base-nucleotide. Use of microarrays to study gene expression and protein expression. Pearson education. alignment. NJ. Blotting techniques and Hybridization techniques. steady state approximation. 1992 Reference Books: 1. rate controlling steps. USA.

process concept. B. and Bischoff K. substance – limiting microbial fermentation. pilot plant and laboratory membrane permeators involving dialysis. J – “Chemical & Catalytic Reaction Engineering”. and trickle bed reactor model. Ceoberry J. 1996 4. McGraw Hill. 1997. Nanofiltration. Surface based solid-liquid separations involving a second liquid. Froment G. Scott Fogler. Octave Levenspiel-“Chemical Reaction Engineering”. H – “ Elements of Chemical Reaction Engineering” – second edition – Prentice Hall of India Pvt. ionic properties and other special characteristics of substances. dual functional filter. centrifugal separators and their relative merits. Charles D. surface properties. John Willey & Sons. F. Theory and equipment used in cross flow filtration. 1999.. Fourth edition. Ceramic membranes. Unit II Types and choice of membranes. Holland – “Fundamentals of chemical Reaction Engineering” Second edition – John willey & sons. Ltd. slurry reactor model. cross flow electro filtration. 2. Outcome At the end of this course. 1995. second edition. – “ Chemical Reactor Design & Analysis”. Third edition – John Willey. 09BT318 MODERN SEPARATION PROCESSES Credit : 4:0:0 Objective To develop skills of students in the area of modern separation processes. Two – phase fluidized bed model. product limiting microbial fermentation Text Books 1. 1986. Reference Books 1. spiral wound and hollow fiber membrane reactors. Economics of membrane operations. second edition – McGraw Hill. the students would have learnt about Recent advances in separation techniques. non-isothermal and non-adiabatic fixed bed reactors. Microfiltration and Donnan dialysis. 3. tubular. Types and choice of membranes. Unit III Karunya University  Page 698  .School of Biotechnology and Health Sciences Department of Biotechnology (BT) Unit IV Analysis and Design of Heterogeneous Reactors Isothermal and adiabatic fixed bed reactors. ultrafiltration. Unit-V Bio Chemical Reaction system. Experimental determination and evaluation of reaction kinetics for heterogeneous systems. Recent advances in separation techniques based on size. reverse osmosis. Plate and frame. Commercial. Commercial processes and its applications Unit I Review of conventional processes. Bailey & ollis – “Biochemical Engineering Fundamentals”. 2. microbial fermentation introduction and overall picture. Enzyme fermentation. 1990.

hydroelectricity. supply and demand. Industrial viability and examples. Unit V Separations involving lyophilisation. Recent advances and process economics. 1982 Reference Books: 1. C.. coal oil. energy storage. Affinity chromatography and immuno Chromatography. Dielectrophoresis. Roussel. Commercial processes. Unit III Karunya University  Page 699  . Pervaporation and permeation techniques for solids. Tata McGraw Hill.W. 1987. Outcome At the end of this course.. Supercritical fluid extraction. structural properties of environment. Types of equipment employed for electrophoresis. Unit IV Controlling factors. uncertainties. King. 1992. various forms. Unit I Energy sources.R. liquids and gases. 2. geothermal. depletion of resources. Handbook of Separation Process Technology. O. ion exchange chromatography and electro dialysis. Bio-geo-chemical cycles and Sources of continuous power. Industrial effluent treatment by modern techniques. Text Book: 1. energy storage. John Wiley. natural gas. other fossil fuels. energy modeling and optimal mix of energy sources. Other separation processes. Zone melting. national and international issues-Ozone layer depletion. Normal adsorption techniques. magnitude and pattern. Adductive crystallization.photosynthesis-Global warming Unit II Forecasting techniques. various forms. input and output analysis. Oil spill Management . Separation Processes. need for conservation. 09BT319 ENERGY MANAGEMENT Credit : 4:0:0 Objective To develop skills of students in the area of energy management. Types and choice of adsorbents. Applications. energy demand. Energy. Types of equipment and commercial process. New York.School of Biotechnology and Health Sciences Department of Biotechnology (BT) Mechanisms.V. Marcel Dekker. the students would have learnt about Energy sources.J. Membrane Science and Technology. Nakagawal. nuclear energy..

MHD.Biogas-pyrolysis . Energy and evolution. Outcome At the end of this course. 1985. conservation applied to Bio-fertilizers. 09BT320 UNIT OPERATIONS AND PROCESSES IN EFFLUENT TREATMENT Credit : 4:0:0 Objective To develop skills of students in unit operations and processes in effluent treatment. capacity. 2. Allyn and Bacur Inc. wind . Solid waste management in Biochemical industries Unit III Filtration – classification of filters – Head loss through filters – Darcy . the students would have learnt about principles of various unit operations used in effluent treatment.. conservation using optimization techniques..H.. growth and change. tariffs. New York. Text Book: 1. food industries. commercial generation of power requirements and benefit.equation. McGraw-Hill Book Co.School of Biotechnology and Health Sciences Department of Biotechnology (BT) Bio-geo-chemical cycles. system cost analysis. 1975. economics. fermentation. Rused.. Energy conservation and utilization. Unit IV Chemical precipitation – phosphate removal – Adsorption – Activated carbon – isotherms – Disinfection – Factors influencing – Breakpoint chlorination – Dechlorination. pharmaceutical industries. cooling tower. Energy generation from Bio-wastes. drying. Unit II Sedimentation – Type of settling – Removal ratio – Clarifier – thickener – Column flotation – air floatation. Cost analysis. cement.. fuel cells. society and environment population and technology. Unit I Selection of unit operations and processes. J. G.. patterns of consumption in developing and advances countries.Screening – Mixing – Coagulation and Flocculation – Flow equalization. Unit V Sources of continuous power. C. Unit V Karunya University  Page 700  . pulp and paper. geothermal. production analysis and production using fuel inventories. Classification. corporate models. Krentz.K. 1976 Reference Books: 1.M. Macmillan Publishing Co. system rate. Energy.Principal type of Reactors. tidal and solar power. Unit IV Chemical industries. input-output analysis. Elements of Energy Conservation. conservation in unit operation such as separation. production rate. Bio-fuel. Gramlay.

quality control. Inc. Disposal. food and pharma industries Unit IV Validation of Biopharmaceutical Facilities Introduction. validationSanitary design principles : Biochemical. Unit III Pharmaceutical water system Grades of water. and Reuse “. water treatment system. water distribution system. Pharma and Biotech industries. Reference Books: 3. Tata McGraw-Hill. John Wiley & Sons. complaints and product recall. Unit II Process economics General fermentation process economics. Materials of construction and properties. Text Book: 1. Casey. Validation Structure. Introduction to GLP and its principles. personal. the students would have learnt about plant design. Third Edition. Unit I Plant Design Fomenter design. reactions vessels. Outcome At the end of this course. capital investment estimate. “Wastewater Engineering–Treatment. 1993. piping and valves for biotechnology. Metcalf & Eddy.School of Biotechnology and Health Sciences Department of Biotechnology (BT) Kinetics of biological growth – Suspended and attached growth processes – Aerobic and Anaerobic – Determination of kinetic coefficients. Why Validation. Utilities for plant design. 1995. production. Unit V Good manufacturing Practices Structure – quality management. Pressure relief system. Text Book: Karunya University  Page 701  . pharmaceutical water system. T.J. Documentation. contract manufacturing and analysis. good manufacturing practices. 09BT321 BIO-PLANT DESIGN AND PRACTICE Credit : 4:0:0 Objective To develop skills of students in bio-plant design and practice. self inspection. sanitary design. Resources for validation. production cost estimate. validation of Systems And Processes including Sip And Cip. when does validation occur. materials usage and cost. process economics.”Unit Treatment Processes in Water and Wastewater Engineering”.Hazardous analysis: Food. Two case studies – one traditional product and one recombinant product. premises and equipment. validation of biopharmaceutical facilities.

etal.. Equipment and Facilities”.1997. Biological Nutrient Removal. Multiple Microbial Activities in Complex Systems. ButterworthHeiemann. the students would have learnt about principles of operation of various reactors used in waste water treatment.Jiing P. Lagoons. 2 Lydersen. “ Plant Design and Economics for Chemical Engineers “. Outcome At the end of this course. Design and Evaluation of Suspended Growth Processes. Unit IV Theory: Modeling of Ideal Attached Growth Reactors. Bio-film Modeling.1994. 2001. Springer. Modeling Suspended Growth Systems. Aerobic Growth of Heterotrophs in a single Continuous Stirred Tank. McGraw Hill.JohnWiley. The why and How ?”. Klaus D. modeling of ideal suspended Growth Reactors. Unit II Theory. Multiple Microbial Activities in a Single Continuous Stirred Tank Reactor. 5th Edition.School of Biotechnology and Health Sciences Department of Biotechnology (BT) 1 Peter. Max S. Techniques for Evaluating Kinetics and Stoichiometric parameters.1993 Reference Books: 1 Seiler. Reactor Receiving Soluble Substrate. Unit III Applications: Suspended Growth Reactors. Stoichiometry and Kinetics of Biochemical Operations. Aerobic – digestion. Unit I Classification of Biochemical operations. B. Aerobic Growth of Biomass in Packed Towers. Unit V Karunya University  Page 702  . fundamentals of biochemical operations. BIOTOL Series. 2 “A Compendium of Good Practices in Biotechnology “.K.. 09BT322 BIOLOGICAL WASTEWATER TREATMENT Credit : 4:0:0 Objective To develop skills of students in instrumentation and biological techniques in waste water treatment. Fluidized Bed Biological Reactors. “Bioprocess Engineering Systems.. and Timmerhaus. Aerobic Growth of Heterotrophs in Rotating Disc Reactors. Activated Sludge. Anaerobic Processes. “Good Laboratory Practice.

Tumour markers UNIT II Cell cycle regulation and Signal Transduction Cancer cell cycles. Metabolic oxidative stress and cancer. JAK-STAT pathway. Rotating Biological Contactor. Genetic changes of selected cancers – Lung cancer. oncogenes and tumour suppressor genes and current concepts in cancer therapy.Daigger. Retroviral oncogenes. Cell cycle control genes and mismatch repair genes.School of Biotechnology and Health Sciences Department of Biotechnology (BT) Applications: Attached Growth Reactors. Biological Wastewater Treatment. Cyclic AMP and activation of CREB. metabolism of carcinogenesis. Graty. Ras/MAPK pathway. principles of physical carcinogenesis. Gastrointestinal cancers and other cancers. G and Lim.P. Retino Blastoma tumour suppressor genes and its significance. cell adhesion. Growth factor receptor kinases and cytoplasmic tyrosine kinases. 09BT323 MOLECULAR AND CELLULAR BASIS OF CANCER Credit: 4:0:0 Objective To develop skills of students in the area of cancer Outcome At the end of this course. 1998 Reference Book: 1. 2nd Edition. DNA methylation and epigenetic silencing of suppressor genes. the students would have learnt about basic principles of cancer biology. angiogenesis. Tumour suppressor gene pathways. C. screening and early detection. Trickling Filter. John Wiley Sons Inc 1989.C. Telomerases UNIT III Oncogenes and Tumour Suppressor Genes Apoptosis. Chemical carcinogenesis.L. UNIT I Cancer Biology: the Basics Introduction. Cellular proto-oncogenes and oncogene activation. promotion. historical perspective and classification of cancer. UNIT IV Understanding Natural History of Cancer Development Free radicals. Text Book: 1. Submerged Attached Growth Bioreactors. Biological Waste Treatment. growth factor receptors and Signal transduction.signal transduction. antioxidants and cancer. Tumour associated antigens and Tumour specific antigens UNIT V Current Concepts in Cancer Therapy Page 703  Karunya University  . Cancer initiation. Marcel D ekker. H. Mizahi A. Growth factors. Breast cancer. Transcription factors. Carcinogenesis: Theory of carcinogenesis. Tumour supressor genes. X-ray and UV radiation-mechanisms of radiation carcinogenesis. Future Challenges. invasion and metastasis. Cellcell interactions. & progression. Fate and Effects of Xenobiotic Organic Chemicals.

Outcome At the end of this course. G. Barry Pierce. Bios Scientific Publishers. The Molecular Biology of Cancer. 2006. diet and cancer. Cambridge University Press. Molecular Biology of Cancer. Cultivation. 4 An Introduction to Cellular and Molecular Biology of Cancer. 1 Reference Books: 1 Macdonald F and Ford CHJ. Modern Biotechnological tools and its influence in Medical and Aromatic plant cultivation. IRLl Press. Natural products and cancer. Genetics as applied to Medicinal herbs. Cancer immunity and strategies of anticancer immunotherapy. 2002. Collection & processing of Crude Drugs.W. surgery. 2002. radiation therapy. Siddha and Unani and its significance Unit II: Medicinal & Aromatic Plants Cultivation and Utilization of Medicinal & Aromatic Plants in India. Immunomodulators in cancer therapy. Applications of Monoclonal antibodies in cancer diagnosis and therapy. Unit III: Tissue Culture of medicinal Plants Plant Tissue Culture as source of medicines. “Virology A Practical Approach”. 2001. 2 Robert G. tissue culture. Ralph E. McKinnell.. Secondary metabolite production in plants. The Biological Basis of Cancer. 09BT324 PHYTOCHEMICALS AND HERBAL MEDICINE Credit : 4:0:0 Objective To develop skills of students in Phyotochemical and herbal medicine. Classification (Taxonomical. Oxford. 2003. Indian System of medicine: Ayurveda. Plant Tissue Culture for enhancing secondary metabolite production (Withania somnifera. “Introduction to Modern Virology”. Text Books Stella Pelengaris and Michael Khan. Blackwell Scientific Publications. the students would have learnt about principles of crude drugs. phytochemical analysis and its applications Unit I: Crude Drugs Crude Drugs – Scope & Importance. Rauwolfia Karunya University  Page 704  . Morphological Chemical. Perantoni. Parchment. Pharmacological). Alan O. Gene therapy and cancer.J And Primrose S. Blackwell Publishers.B. 2 Maly B. New York 2003. 3 Dunmock N. Oxford. J Oxford Medical Publications.School of Biotechnology and Health Sciences Department of Biotechnology (BT) Different forms of therapy – Chemotherapy. medicinal and aromatic plants.J.

L.extraction methods. gene transfer methods. Text Books: 1 Pharmacognosy. Biocides. K:J.School of Biotechnology and Health Sciences Department of Biotechnology (BT) serpentina. Anticancer. PCR. Microscopic. enzymes as molecular tools. B. Antiinflammatory. 4th Ed. Biofungicides. Biogenesis of Phytopharmaceuticals. Antidiabetic.). Pulsed field electrophoresis. A. 09BT325 ADVANCED PLANT BIOTECHNOLOGY Credit : 4:0:0 Objective To develop skills of students in the area of Plant biotechnology. Agarose Gel Electrophoresis. Reference Books: 1 Hornok. Gokhale Nirali Prakashan. Purohit & S. C.). . Lectins. Glycosides . Pharmacognosy – William Charles Evans. Papaver. U.plant transformation technology and its applications. Andrographis paniculata. K. the students would have learnt about principles of basic techniques of genetic engineering.extraction methods (Taxus. Characterization of drugs. 2 Trease & Evans. Outcome At the end of this course. Kokate. Aloe. Southern. Analgesicdrugs. Alkaloids . Catheranthus roseus.extraction methods (Digitalis. Karunya University  Page 705  . Assay of Drugs – Biological evaluation / assays. Resins. Types of Phytochemicals: Carbohydrates & derived products. Flavonoids. Preliminary screening. Microbiological methods. UNIT-I: Genetic engineering: Introduction to Gene Manipulation. Cinchona). Nutraceuticals and their significance. Volatile Oils . functional sequencing of cDNA Expression libraries. Tannins (Hydrolysable & Condensed types). Physical & Chemical). RNA).extraction methods. Mentha). Unit V:Applications of Phytochemicals Application of phytochemicals in industry and healthcare. Dioscorea sp.Converting mRNA transcripts into cDNA libraries. 1996 2 Natural Products in medicine: A Biosynthetic approach Wiley 1997. Unit IV: Analysis of Phytochemicals Methods of Drug evaluation (Morphological. Chichister. (ed. P. Dioscorea. Biopesticides. Northern and western blotting. Wiley & Sons 1992. Harcourt Brace & Company 1989.extraction methods (Clove. 14th ed.) Cultivation & Processing of Medicinal Plants. Basic Techniques: Isolation and purification of Nucleic Acids (DNA.

microinjection. Ltd. viral vectors and their applications. 5 Adrian Slater. Molecular Biotechnology. blue rose.School of Biotechnology and Health Sciences Department of Biotechnology (BT) UNIT-II: Methods in genetic: enzymes as molecular tools . England. Nucleic acid microarray. Sambrook. Outcome: 1 Karunya University  Page 706  . radiolabling and alternatives. Edible vaccines. use of ACC synthase. Pearson Education limited. 2008. Hughes. Reference Books: Plant molecular genetics by Monica. nucleocapsid gene. Fritsch and T.C. 09BT326 BASICS OF CHEMICAL ENGINEERING Credits 3:0:0 Objective: This course is intended to provide beginning engineering students with a clear overview of the field of chemical engineering so they can confidently decide if they want to pursue chemical engineering as a profession. Particle bombardment. and Mark Fowler. Ignacimuthu S. Physical method of gene transfer. UNIT-III: Methods of gene transfer in plants: transient and stable gene transformation. Pvt. electroporation. glyphosate. molecular probes. PR proteins. use of Ti and Ri as vectors. 2001. J. UNIT-V: Application of plant transformation for productivity and performance: herbicide resistance. S. coat protein mediated. binary vectors. Cold Spring Harbor Laboratory Press. UNIT-IV: Plant transformation technology: Agrobacterium tumefaciens as plant genetic engineer: features of Ti and Ri plasmids. chemical mediated transformation. 1-3 beta glucanase. atrazine. 2 Molecular Cloning: a Laboratory Manual. Introduction to Plant Biotechnology. DNA modifying enzymes and their applications in recombinant DNA technology.J. ACC oxidase. nucleotide labling. A Text Book of Biotechnology. Nigel Scott.. Bt genes.. antifungal proteins. disease resistance. polygalacturanase.uses of restriction enzymes in plant genetic engineering. 2004. Oxford and IBH Publishing Co. 2001. Plant Biotechnology.S. 4 Glick and Pasternak. E. phosphoinothricin. 1999. ADP glucose pyrophosphatase. Dubay. Chawala. New Delhi. nematode resistance.Chand & Campany Ltd. insect resistance. Maniatis. silicon carbide mediated and floral dip method. New York. Ltd. The genetic manipulation of plants. sufonyl urea. Golden rice. Pvt. Oxford and IBH Publishing Co. thionins.. Preparation of probes. Text Books 1 H. virus resistance. carbohydrate composition and storage.F. gene knock out and silencing and RNAi. chitinase. A. Oxford University Press . 2 S. Plant Biotechnology. New Delhi 2002. 2001 3 R.

weight. rate equation. order of reaction. fifth edition.C. Basics gas calculations-mole. and Vora S. design calculation – Mc Cabe Thiele method. 2000... Sixth Edition. Felder R. Ideal gas laws. 2004. weight percentage molarity. fluid flow pattern. John Wiley and Sons. Prentice-Hall of India Pvt. 2004. Ltd.concept. John Wiley. molality. Himmelblau D. concept of NTU and HTU. mole percentage. and Rousseau R. performance equation for CSTR. 2006 Reference books: 1.M. flow control. Distillation. Unit-V Chemical Reaction Engineering Law of mass action. Octave levenspiel. “Basic Principles and Calculations in Chemical Engineering”.PFR. drying and leaching.. Inc. “Unit Operations in Chemical Engineering ". concept of evaporator. Material balance. types of fluid.L. heat transfer coefficients. definition of space time. poncho savant method. heat transfer – modes of heat transfer.W.M.. normality. energy balance.I. 2.School of Biotechnology and Health Sciences Department of Biotechnology (BT) At the end of the semester the student will develop basic skills in problem solving. Tata McGraw-Hill Pub. adsorption. seventh Edition. Mccabe. fluid flow. computation. W. Third Edition. fluid flow measurements and transportation of fluid. McGraw-Hill. Chemical reaction engineering. Fourth Edition.. equipments – heat exchanger (double pipe. elementary and non elementary reactions. Unit-II Fluid mechanics Concept of fluid. types. Bhatt B. Ltd.types of reactions and reactors. 3.2006 09BT327 RESEARCH METHODOLOGY Credit: 3:0:0 Karunya University  Page 707  . Smith J. plate type). Unit-I Process calculations Basic units and dimensions. “Elementary Principles of Chemical Processes”. filtration and mechanical separation Unit-IV Mass transfer operations Diffusion in fluids – molecular and eddy diffusion. “Stoichiometry”. process design and communication that will help them in all future engineering courses. Unit-III Unit operations Concept of unit operations. 2. properties of fluid.M.. shell& tube. LMTD. Basic concept of absorption. mean residence time Text books: 1. Co.

recombinant material safety. bio safety. UNIT IV: Biostatistics Definition and scope. Microscopic techniques. New Delhi. Bibliography and Reference. Significance of research. Genetic engineering and biowarfare. Biotechnology’s impact on society. Ranjit Kumar. New Delhi. UNIT V: Manuscript/ Thesis Writing Research report: components. the Genetic revolution: Ethical issues. 2006. Jerrod H. 2002 2. Methods and techniques. Graphs. Kothari. UNIT I Ethical issues and Biosafety Safety and risk of bioethical concerns. Donald H. The future of bioethical biotechnology. 3. McBurney. Diagrams). Sage publications. Types of biological data – Collection and presentation of data (Table. Text Books: 1. Singapore. Inc. Sage Publications. Ltd. Outcome The students will be familiar with experimental design data collection and Biostatics. London. UNIT II: Research Problems and Experimental Design Essential steps in research. Literature collection. Immunotechnoques and chromatographic techniques. Manuscript/research article preparation. Packing and sterilization of glass and plastic wares for animal tissue culture Karunya University  Page 708  . patent. Publishers. Al. defining the research problem. Press. Literature citation. Zar (1999) Biostatistical analysis by. Doing Management research. et. research methods. Citation index of journals UNIT III Instrumentation Techniques Spectrophotometric methods. 09BT328 ANIMAL AND PLANT TISSUE CULTURE LAB Credits 0:0:2 List of Experiments 1.School of Biotechnology and Health Sciences Department of Biotechnology (BT) Objective To impart the knowledge about the method by which the research has to be carried out. Reference Books: 1..R. Probability analysis – Testing of significance – Goodness of fit (X2 test) – Student’s ‘t’ test – Simple & multiple regression – Correlation: Canonical Correlation – Correlation coefficient– ANOVA (one way and two way analysis of variance). London. London. Research/Experimental design. New Age Internation (P) Ltd. Review monographs. Thomson Asia Pvt. Standard operation protocol. Impact factor of journals. 2001. Format of thesis and dissertation. 2. C. Raymond – Alain Thie’ tart. Laboratory safety. IPR. Prentice Hall International. IInd edition (2004) Research methodology. Research methodology.

2006. Oxford. Animal Cell Culture: Practical Approach. 3. 5th Edition. John R. Genetic transformation and hairy root production 13. Culture of Animal Cells. 2006.2005. organogenesis and rooting media 8. Masters. Preparation of reagents and media for cell. Protoplast isolation and protoplast fusion 12. Cryopreservation 7. Karunya University  Page 709  . M. Sterilization and inoculation of leaf.School of Biotechnology and Health Sciences Department of Biotechnology (BT) 2. Bone marrow cell isolation. Clynes. Springer. R. 2ndt Edition. Haploid production References 1. processing and culture 5. Macrophage culture and phagocytosis assay 6.W. Callus and Sub culturing and etiolation of explants 10. Rooting. Freshney. 2. Spleen cell processing and culture of splenocytes 4. Animal Cell Culture Techniques. Preparation and Sterilization of Basal. tissue culture 3. 3rd Edition. cotyledon and nodal explants 9. Wiley-Liss. Micro grafting and hardening of explants 11. I.

BIOTECHNOLOGY 0 Karunya University .

Unit II Structure and Properties of Carbohydrates. Buffer-definition. Unit III Structure and Properties of Proteins and Nucleic Acids Structure and properties of amino acids. lipids. glycogenesis and glycogenolysis. classification. water as reactant in living organisms. peptides. stereospecificity. Fitness of aqueous environment for living organisms. Introduction to Enzymes-Nomenclature. action-units. enzyme reaction rates. nucleoprotein complexes. structure. pyrimidines. Structure and properties of fatty acids. lipid aggregates. enzyme catalysis. oligo and polysaccharides. biological role of enzymes. sphingolipids. buffering in biological system. specificity. phospholipids. 3D structure. Water-Ideal biological solvent. nucleosides. properties and functions of vitamins. chemistry of nucleic acids. proteins. modified amino acids. Classification. gluconeogenesis. biomedical . composition and function of various biomolecules Outcome At the end of the semester the students will enable to understand the metabolism of carbohydrates. nucleotides. di. Structure and properties of mono. glycolipids and cholesterol. macromolecules. molality. Inborn errors of 1 Karunya University . percentage dilution. buffer preparation Normality. Buffer and pH and –pH definition and scale. Unit I Introduction to Biomolecules Biomolecules-functional groups. proteins and the bioenergetics. glyoxylate cycle. Vitamins-occurrence. Structure properties and functions of purines. pentose phosphate pathway. Unit IV Metabolism Of Carbohydrates. ribonucleic acids and deoxy ribonucleic acids.Effects on dissolved biomolecules. Lipids and Bioenergetics Lipid Metabolism: Biosynthesis and biodegradation of fatty acids and cholesterol. Carbohydrate Metabolism: Glycolysis-fate of pyruvate.ADDITIONAL SUBJECTS Code 10BT201 10BT202 10BT203 Name of the Subject Basic Engineering Biochemistry Genetic Engineering and Bioethics Cancer Biology Credits 4:0:0 4:0:0 3:0:0 10BT201 BASIC ENGINEERING BIOCHEMISTRY Credits: 4:0:0 Objective To study about the structure. molarity. chemical reactivity. Lipids and Enzymes Classification of carbohydrates. TCA cycle. polynucleotides. important biological buffers. Ionization of water. Classification of lipids.

. Biochemistry. Inborn errors of their metabolism. energy rich compounds. A. prokaryotic and eukaryotic expression vectors.. genomic library. Tyr. Rodwell. Taqman assay. Construction of cDNA and genomic libraries. 1995. 2. Met. P. RAPD. Lehninger. Reference Books: 1. Bioenergetics: redox biochemistry. Prentice Hall International. M. (2000). Insect.. Harper’s Biochemistry. Nelson. R.K. Restriction mapping. Invitro fertilization.A. D.. transgenic and knockout animals. and Cox. L. gene-therapy. New York. respiratory chain and oxidative phosphorylation Unit V Metabolism Of Proteins And Nucleic Acids Biodegradation of proteins and nucleic acids. Lys. methods of nucleic acid sequencing.W. design of linkers and adaptors. Screening of libraries with DNA probes and with antisera.Leu. Applications. Nested PCR. r-DNA technology. Diagnostic importance of PCR. 2nd Edition. Chand publication. WH Freeman & Co. Granner. Biosynthesis and biodegradation of important amino acids. L. Jain and Jain (2008). M.Steps. Text book: 1. Glutamate and Cys-Urea Cycle. Trp. 2000..Recombinant cytokines and antibodies. 3. V. Mayes. stem cell therapy. triose phosphate cycle in plants. 2 Karunya University . Principles of Biochemistry Third Edition (Freeman Publishers). embryo transfer technology. RACE PCR.metabolism. mutation. PCR and other applications of genetic engineering and also ethical issues relating to genetically manipulated products Unit I Basics of Recombinant DNA Technology Overview of recombinant DNA technology . (2000). Yeast and Mammalian vectors. 10BT202 GENETIC ENGINEERING AND BIOETHICS Credits: 4:0:0 Objective Helps the student to understand and apply this knowledge in research to study the molecular mechanism of DNA and RNA synthesis and Protein synthesis. Thr. Phe. transamination. Voet and Voet. B. Restriction and modifying enzymes. Site Directed Mutagenesis. vaccines. Outcome At the end of the semester the students would have learnt about r-DNA technology. Biochemistry. John Wiley & Sons Inc. 4th Edition.K. Unit II: Vectors and Cloning Characteristics of plasmid and phage vectors. Murray. Biochemistry. Unit III Polymerase Chain Reaction & Construction of Libraries Inverse PCR. Lubert Stryer. purines and pyrimidines. 2.. Cloning in plants.. Molecular beacons.

2008. An Introduction To Genetic Engineering ". 2.risk analysis and assessment. Three step theory of invasion. Cell cycle. RCGM. 1-3. 2002. Moore DD. Definition of GMO & LMO. Oncogenes. Cancer: Types of cancer. Sambrook et al Vol. Biodiversity and farmers rights. Check points in cell cycle. International conventions. Patents and methods application of patents. Tumour specific antigens. Tissue Inhibitors of Metalloproteases (TIMPs).Unit IV Bioethics & IPR Bioethics: The legal and socioeconomic impacts of Biotechnology. 1987 3. Saleesha A. Blackwell Science Publications. Cancer screening and early detection. Ansubel FM. GEAC etc for GMO applications in food and agriculture. Environmental release of GMOs. Academic Press. Unit V Biosafety and rDNA guidelines Biosafety regulation and National and international guidelines. cancer metastasis and angiogenesis. Reference books: 1. Intellectual property rights. “Molecular Cloning” 10 BT 203 CANCER BIOLOGY Credits: 3:0:0 Objective: The aim of the course is to provide basic knowledge on cancer biology Outcome: At the end of the semester the students would have learnt about Carcinogenisis. Tumour markers. Unit I Fundamentals of Cancer Biology Mitosis and Meiosis. 3 Karunya University . Brent R. GATT. Tumour suppressor genes.Stanely. Old RW. Kimmer AR. Greene Publishing Associates. Unit II Carcinogenesis Environmental factors in cancer development. Kingston RE. Berger Sl. Roles of Institutional Biosafety committee. r-DNA guidelines. Unit IV Cancer development Metalloproteases (MMPs). Experimental protocol approvals. TRIPS. 2. Theory of carcinogenesis. Vol 152. Total : 65 hours Text Books: 1. NY. levels of containment. "Principles Of Gene Manipulation. physical and chemical carcinogens. "Current Protocols In Molecular Biology ". “Bioethics”. Immunohistochemistry. Wisdom educational service. Early diagnosis of cancer and about treatment methods to cure cancer. Apoptosis and cancer: Basic concepts. 1988. Radiation and cancer. Legal implications. Primrose SB. "Methods In Enzymology". free radicals in carcinogenesis Unit III Basics of Molecular biology of cancer Tumour associated antigens.

Oxford Medical Publications. Molecular Biology of cancer: Mechanisms. Robert A Weinberg. 1991. 2006 2. USA. Reference Book: 1. Gene Therapy. Lauren Pecorino. “An Introduction to Cellular And Molecular Biology of Cancer”. Targets and Therapeutics. Surgery. Text Books 1. New York. Garland Science Publishers. Oxford University Press Inc. 2008. The Biology of Cancer.Unit V Cancer Therapy Treatment modalities available for cancer: Radiation Therapy. Chemotherapy. 4 Karunya University .

SCHOOL OF BIOTECHNOLOGY  & HEALTH SCIENCES  DEPARTMENT OF BIOTECHNOLOGY        .

REVISED & NEW SUBJECTS Code 09BT329 09BT330 09BT331 09BT332 10MB201 10MB202 10MB203 10BT301 10BT302 10BT303 10BT304 10BT305 10BT306 10BT307 10MB301 10MB302 10MB303 10MB304 10MB305 10MB306 10MB307 10MB308 10MB309 10MB310 10MB311 10MB312 10MB313 10MB314 10MB315 10MB316 10MB317 10MB318 10MB319 10MB320 10MB321 10MB322 10MB323 10MB324 Subject Molecular Biology and Nanotechnology applications in Cell Biology Cancer Chemotherapy Mental Health Care Biofuel Cells Cell Biology Microbiology and Immunology Biotechnology Lab – I Advanced Engineering Biochemistry Advanced Biochemical and Bioprocess Engineering Process Equipment Design and Drawing Metabolic Regulations and Engineering Advanced Immunotechnology Industrial Safety Engineering Process Instrumentation. Dynamics and control Nutritional Biochemistry and Physiology Advanced Intermediary Metabolism Medical Microbiology Medical Bioinformatics Molecular Cell Biology Nutritional Biochemistry and Physiology lab Microbiology lab Biomedical Instrumentation Advanced Clinical Biochemistry Advanced Endocrinology Medical Biotechnology Genetic Engineering and Medical ethics Clinical Biochemistry and Experimental Hematology lab Endocrinology lab Immunology and Immunotechnology Clinical Pathology Immunology Lab Medical Lab Technology Lab Neuroscience Medical Lab Technology Complementary and Alternative Medicine Molecular Medicine Cell Culture Techniques Pharmacology and Pharmacognosy Credit 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 0:0:2 4:0:0 4:0:0 4:0:0 4:0:0 4:0:0 3:0:0 3:0:0 4:0:0 4:0:0 4:0:0 3:0:0 3:0:0 0:0:2 0:0:2 3:0:0 4:0:0 4:0:0 3:0:0 3:0:0 0:0:2 0:0:2 4:0:0 4:0:0 0:0:2 0:0:2 3:0:0 3:0:0 3:0:0 3:0:0 3:0:0 3:0:0 1  Department of Biotechnology   .

use of PCR in molecular diagnosis. Unit IV: Nanotechnology Introduction and scope of nanotechnology in medicine. ELISA. IPR. Transport mechanisms. Outcome: The Student will learn the principle of application of molecular and nanotechniques and its exploitation through cell biology. ethical issues in nanotechnology. RIA. molecular tools for early diagnosis of cancer. in-situ hybridization. maintenance and preservation of various types of culture suspensions. Molecular techniques. detection and identification of oncogenes. immune diffusion and immune electrophoresis. monoclonal and polyclonal antibodies production and application. immune histochemical analysis. Signal transduction. Department of Biotechnology   2  . AFLP. Unit I: Cell Culture Introduction to basic tissue culture techniques. Synthesis and characterization of nanoparticles. blotting techniques. application of nanotechnology. Different forms of cancers. cultures as a source of valuable products. drug delivery system using nanoparticles.RAPD. animal cell culture. Unit II : Cell Biology Structure and functions of cell organelles. establishment of primary cell culture. RFLP. Unit III: Molecular Biology And Immunotechnology Gene sequencing. detection mechanism for cancer using biochemical assay. types of nanoparticles. Unit V: Therapy Gene therapy. Cell signalling. chemically defined and serum free media.10MB325 10MB326 10MB327 10MB328 10MB329 10MB330 Molecular Biotechnology Molecular Physiology Biomaterials Biological and Biomedical Nanotechnology Biotechnology Lab – II Biotechnology Lab – III 4:0:0 4:0:0 4:0:0 4:0:0 0:0:2 0:0:2 09BT329 MOLECULAR BIOLOGY AND NANOTECHNOLOGY APPLICATIONS IN CELL BIOLOGY Credit: 4:0:0 Objective: The aim of this course is to provide knowledge in the applications of molecular aspects and nanotechnology in cell biology. toxic effects of nanoparticles. nanobiosensors.

Ionizing radiations. Bios. Physical and chemical carcinogenesis.Text Books 1. Rantner M. Role of flavonoids and polyphenols in cancer. 3. Different types of cancer therapy: Surgery. vegetables and cancer. 3. Blackwell Publishers. 2006. Unit II: Food and Cancer Diet and cancer prevention. 4. Molecular biology of cancer. Cancer initiation. Unit IV: Cancer Chemotherapy Department of Biotechnology   3  . P Ramadass – Animal Biotechnology “Recent concepts and developments” MJP Publishers. Oxford Medical Publication. 2002. 2003. Radiation therapy and chemotherapy. Types and process of carcinogenesis. Unit I: Carcinogenesis Carcinogenesis: Steps in carcinogenesis. Reference Books: 1. Stella Pelengaris and Michael Khan. Prentice Hall. Combination chemotherapy. Fruits. 2. Viola vogel –“ Nanomedicine and Nanotechnology” John willey and Sons Ltd. An introduction to cellular and molecular biology of cancer. Outcome At the end of this course. 2008. J. promotion and progression stages.. Third edition . Adjuvant chemotherapy. The Molecular Biology of Cancer. 2004. Unit III: Cancer Therapy Various types of cancer treatment. Environmental factors and causes for different types of cancer. Good Sell – “Nanobiotechnology”. Role of stem cells in cancer. b. Role of Drugs in Cancer Treatment – a. John willey and Sons Ltd. Food. Gene therapy. Macdonal F and Ford CHJ. 09BT330 CANCER CHEMOTHERAPY Credit: 4:0:0 Objective To develop interest and knowledge of students in the area of cancer chemotherapy.. Scientific publishers. the students would have learnt about basic principles of cancer therapy. 2. 2008. Ratner D – “Nanotechnology: A Gentle Introduction to the next Big idea”. Types of cancer in men and women. screening procedures and current concepts in cervical cancer and therapy. Various carcinogens present in the environment.

Role of flavonoids and polyphenols in cervical cancer. Reference Books: 1. Natural Products in medicine: A Biosynthetic approach Wiley 1997. surgery and chemotherapy in cervical cancer. Stella Pelengaris and Michael Khan. Parchment. McKinnell. 2006. Detection techniques. Alan O.General principles of cancer chemotherapy. The Biological Basis of Cancer. Mental disorders. Prevention. chemotherapy of lung cancer. Ralph E. Cervical cancer vaccines. Cervical cancer therapy. New York 2003. Perantoni. Screening procedures – types. 09BT331 MENTAL HEALTH CARE Credit: 4:0:0 Objective To develop skills of students in the area of Mental health care Outcome At the end of this course. Barry Pierce. Significance of nutrients and Neurotransmitters and current concepts in herbal therapy. Blackwell Publishers. Awareness of screening procedures in developing and developed countries. 2. Robert G. Chemotherapy induced nausea and vomiting. HPV and its role. Life cycle of HPV. commonly used cytotoxic drugs. 2003. Unit I : Introduction to Mental health and Classification of mental disorders Definition and needs of mental health. Side effects of cancer chemotherapeutic drugs. The Molecular Biology of Cancer. Types of HPV. the students would have learnt about basic principles of Mental health care. G. Text Books 1. breast cancer and cervical cancer. Life style. J Oxford Medical Publications. An Introduction to Cellular and Molecular Biology of Cancer. 2. Cambridge University Press. Unit V: Cervical Cancer Cervical cancer. Causes and Etiology of cervical cancer. Abnormality-Introduction-What is abnormality? Models: Biological: Interpersonel Classification of mental disorders-DSM-IV-ICD-10 Unit II : Mental disorder and Biological causes Information about mental illness and brain. Brain function and alteration Mental stress-Types and causes Mood disorders-Depression-Major depressive disorders-Types of depression-Biological causes of Depression Department of Biotechnology   4  . Global incidence and status in India.

W. Text books: 1.Comer (1996). 09BT332 BIOFUEL CELLS Credit: 4:0:0 Objective: To develop interest and knowledge of students in the area of Biofuel cells Outcome: At the end of this course.Short review of Hormonal imbalance in depression Unit IV : Nutrient role in mental health.NIMH. 1.Freemann company edition. 2. the students would have learnt about basic principles of Biofuel cells and current concepts in Biofuel cells Unit I : Introduction to Biofuel cells and Biomass Biofuel cells – A general account – Environmental Impact – Technology aspects of biofuel cells – Biofuel cell classification – Biocatalyst . Monday.The chemical imbalance in mental health problems.New Delhi 3. 16 February 2009.Second Generation biofuels – Importance of biofuel energy – Potential biomass for fuel generation – Types and composition of biomass – Pretreatment – Biomass feedstocks – Biomass to energy conversion technologies – Energy and fuel using microorganisms Unit II : Construction of Biofuel cells Department of Biotechnology   5  .Fundamentals of Abnormal psychology.H.Sarason I.The science of mental illness. 3.P.Ronoldo. Joseph M.James Lake-2006 References: 1.The problem of Maladaptive behavior .Essential Fatty acids and importance.2000 2.Necessity of Herbal therapy in mental health Antidepressive medicinal plants. The chemical imbalance in mental health problems Neurotransmitters level and emotional health Dopamine-Serotonin-Nor epinephrine-GABA levels .Importance of balanced diet – Minerals and their needs-Vitamins and implications.R(1998). 2.G and Sarason B.New Delhi 2. Teacher’s guide.eighth edition.F.The vital role of Hypericum perforatum Active constituents and functions of Hypericum perforatumSuccessive therapy to heal depression .Abnormal psychology .hd .Pentile hallof India .Schizophrenic Disorder –Characteristic and probable causes –Major Subtypes of Schizophrenia-causes-Biological causes of Schizophrenia Unit III : Neurotransmitters in mental health Neurotransmitters-Importance and implications.Carver. 1.Nutritional deficiency in depressive and Schizophrenic Disorder Unit V : Herbal therapy for mental health.Text book of Integrative medicine for Mental Health care.

1992 References: 1. Settle. Elsevier’s Science & Technology Rights Department in Oxford. U. T. “Fuel cell fundamentals”.SIMS. ”Encyclopaedia of Materials Characterisation”.. UK 2009 2. a division of Reed Publishing(USA) Inc. Operations and Performance of biofuel cells – Proton exchange membrane fuel cell – Nanocomposite membrane – Fabrication of nanofibre and nanowire – Microfabricated fuel cells – Microchips based fuel cell design – Microencapsulation – Enzymatic biofuel cell designs – Nanomaterials in enzymatic fuel cell designs Unit III : Voltage and Power Generation Voltage and current – Maximum voltage based on thermodynamic relationships – Anode potentials and enzyme potentials – Voltage generation by fermentative bacteria – Calculating Power – Coulombic and energy efficiency – Polarization and Power density curves Unit IV : Characterization Techniques Fundamental Electrochemical Variables: Voltage. New Jersey.Biohydrogen – Waste water treatment – Water desalination – Biosensors – Robots – Renewable energy generation . “Microbial fuel cells”. “Electrochemical Methods : Fundamentals and Applications”.TEM instrumentation and analysis – Chemical Determinations: AES.Porosity determination by BET surface area determination – Gas Permeability – Structure Determinations: XRD. Frank A. 2006 2.Biofuels for transportation sector – Biofuel cells in the future Text Books: 1.R. Logan. ISBN: 0-471-04372-9. Hobart H.SEM. Dean. Bruce E. Inc. Jr. Shaun Wilson. Lynne L. ISBN: 978-0-12-374713-6. 2008 3. John A. Current and Time – Basic fuel cell test station requirements – Electrochemical Impedance Spectroscopy – Current interrupt Measurement – Cyclic Voltammetry . Ryan o’ Hyre. Bard and Larry. New Jersey. Willard. 1986 4. “Instrumental Methods of Analysis”. John Wiley & Sons NY. Inc. ISBN-10 047174148-5 John wiley & Sons. Charles A.S.XPS instrumentation and analysis Unit V : Applications of Biofuel cells Bioelectricity generation the process of electrogenesis – Energy sustainability of the water infrastructure . ISBN-1397-0-471-74148-0.faulkner. Meritt.. ISBN-0-7506-9168-9 Butterworth Heinemann.Construction.S. 2nd Ed.A. Allen J. ISBN-81-239-0943-8 Wadsworth Publishing Company. Hoboken. Zhao “Micro Fuel cells Principles and Applications”.Richard Brundle. Evans. Hoboken.. 2001 Department of Biotechnology   6  . ISBN-978-0-470-23948-3 John Wiley & Sons. C.

Cell adhesion functional aspects of cell adhesion molecules and its diseases. self-renewable and regenerating tissues –Stem cells in diseases and therapeutic uses of growth factors Unit II: Cytoskeleton and cell nucleus Introduction. Biotechnology Advances.Zhuwei Du a. Microtubules and associated proteins . Unit IV: Transmission signals Recognition of extra-cellular stimuli .Control on formation of second messenger molecules.CDKs and Growth control – Apoptosis: common molecular mechanism in diseases . Tingyue Gu. Cell surface .Germ Cells and Fertilization Primodal germ cells-origin of stem cells. ICS-UNIDO.Eggs and Sperm .Actin and its associated molecules. Ultrastructure and differences between prokaryotic and eukaryotic cells Cytoskeleton.Cell organelles and envelopes. Course Outcome: The candidates will be familiar with the basics of the cell both prokaryotic and eukaryotic cells and its analysis by molecular techniques.Integrins as trans-membrane heterodimers . Myosin . Sivasamy Arumugam. Department of Biotechnology   7  . 2007 4.Cell nucleus: chromatin and chromosomes .Sorting in the golgi complex. ”A state of the art review on microbial fuel cells: A promising technology for wastewater treatment and bioenergy”. Extra-cellular matrix and plasma membrane .Elastins and tissue elasticity . role of cyclin dependent kinases – cell cycles. ”Biofuel Production Technologies” Area of chemistry. Unit III: Cell reproduction and apoptosis Mitosis and meiosis.Regulated endocytosis: receptors for EGF and transferrin –cystic fibrosis.Determination of cell shapes Intermediate filaments . Haoran Li a. 25: 464–482.Functions of genetic materials in heredity.Extra-cellular matrix and cell shape during cell proliferation . Unit V: Applications of cell biology The extra-cellular matrix of animals: Glycosaminoglycans (GAGs) . and cell surface ion channels. 10MB201 CELL BIOLOGY Credit: 4:0:0 Course Objective: To study the morphology and functions of the cell and its components and to analyse signalling pathways. 3. 2007. Sergey Zinoviev.Cells and organization of collagen fibrils . Unit I: Cells and stem cells Introduction. and Stanislav Miertus.Transmission of receptor signals to the cell interior: General principles . sustainability and regeneration and application.

Johnson.Staphylococcus (MRSA). Viral. Zipursky. Hepatitis.Krieger.APC. classification and mode of action of antiboditics – Antimicrobial resistance – Tests for sensitivity to antimicrobial agents –SCP. 1991 2. 2008 2.Ed. Pseudomonas (Urinary track Infection). Matsudairia. Cano. K.HIV. California.S. Innate and Acquired immunity – Humoral and Cell mediated immunity – Organs and cells involved in immune responses – Hemopoitic stem cells. Scott.2003 Reference books: 1. antibodies and complements Types of antigens and its characteristics. This paper will give vast idea of antigens.5. N. cilia and flagella – Structure and function of pili and capsule – Sporulation and microbial nutrition. 2009 3. Light microscopy types and application. S. clonal selection (Monoclonal. Cell and molecular biology. Karp. Darnell.J.6. Wadsworts Inc.Amebiosis. Lodish. Wolfe. W. USA.Wine and ethanol production. Department of Biotechnology   8  . Poly clonal) – Antibody specificity – Antigen specific receptor of antibodies – Structure and functions of immunoglobulins –Complement system. Puritt.Candidiosis. Molecular cell biology. Molecular and cellular biochemistry.L. R.1999 10MB202 MICROBIOLOGY AND IMMUNOLOGY Credit: 4:0:0 Objective: To study the basics of microbiology and immunology.Text books: 1. Malaria. Unit III: Outlines of immunology Overview of immune system. Unit IV: Antigens. Activation and maturation of lymphocytes and monocytes. Stansfield.– Antimicrobial chemotherapy – Source. Fungal. Molecular and Cell Biology. McGraw Hill. Outcome: The candidates will have background and can narrow down to their stream of research in immunology and microbiology. Ed.D. Histology and cell biology. Kaiser.Bacterial.Microbe interaction Microbial diseases.6. Unit II Application of Microbes Host.L. G. Antibody synthesis. John Wiley &sons. Berk. Protozoan.concepts and experiments. aspergillosis.Ed. Colome. Unit I: General Microbiology History and scope of microbiology – Classification of microbes and binomial nomenclature Culturing methodology – Sterilization and handling of microbes – Structure and functions of cell wall. J.2.E. Ed. Williams and Wilkins publishers. antibodies and analysis by various immunotechniques.

Chan ECS. blood grouping. and Reid. Immunology. Weir and J. cell signaling. Inc.. Bruce Alberts. Microbiology.N. 2010 2.Talaron.Immune disorders and Autoimmunity – Major Histo-compatibility complexes – Immunodeficiency and immunotherapy – Hypersensitivity –– Immunological techniques: RIA. W. Keith Roberts. Molecular biology of the cell. 2003 4. Ed. Churchill Livingston.B.. Immunocytochemistry.Unit V: Immune System Antibody synthesis. Pelczar MJ. Ed. Fluorescence antibody technique.Primary and secondary interactions of antigens and antibodies.K. Ivian Roitt.7. Pober. Cellular and molecular immunology. Prasad. Nepal. A. New Delhi. 2007 10MB203 BIOTECHNOLOGY LAB-I Credit : 0:0:2 12 experiments will be notified by the HoD from time to time 10BT301 ADVANCED ENGINEERING BIOCHEMISTRY Credit: 4:0:0 Objective To enable the students to understand the various metabolic pathway and cell signaling Outcome The students will be familiar in transport mechanism and various metabolic pathways and their significance Unit I :Complex Carbohydrate and Regulation of Carbohydrate Metabolism Complex Carbohydrates-Structure.3.C. Kathmandu. 1997 Reference 1. Blackwell Scientifics. and Krein NR. Lichtman.8. Abbas. Ed. India. New York. 8. Foundations in microbiology.4. ELISA. Saunders Co. glycolipids and lipopolysaccarides. K. Ed.M. A. Structural and functional relationships in complex carbohydrates and their role in cell-cell interaction. Ed. 3. W. Essential immunology. Brigham Narins. 2001 2. Garland pubs. Ed.1996 3. Thomas Gale publishers. D. A. Tata McGraw Hill. properties and function of proteoglycan. Interlinkage of Department of Biotechnology   9  . recognition markers and cell-cell adhesion. 1994 3. Immunoblotting.11 4. glycosaminoglycan.Casita. James D Watson.K. A Text book of biotechnology.S. B.. Martin Raff. Julian Lewis. Dennis Bray. J. clonal selection . 8. Stewart. Ed. Brown publishers. glycoprotein. Ed. Budha academic enterprises. World of microbiology and immunology. 3. Ed. Text books: 1. New York.

M.Peter A. quinones.glycosylation. isolation. Department of Biotechnology   10  . Harper’s Biochemistry – (2000).Nepal . glutathione reductase.Ltd.O. Homologous proteins.Daryl K. 2004. Victor W. L.Krebs bicycle. Md Murray. Ketone bodies production and their role. Coenzymes-role of vitamins as co-enzymes-B-complex. Lehninger.. physiological role and mechaninsm of action of Hormones that bind to intracellular receptors.. hydrogen peroxidase. Desaturation of fatty acids. Biochemistry. Unit II: Proteins Characterization and Structural elucidation of proteins. Budha Academic Publishers Pv. Jain and Jain (2008). steroid hormones.Appleton and Lange. & Metabolism. L. glucocorticoids. Biosynthesis of membrane phospholipids. Lipid extraction. dolichols and fat soluble vitamins. Structural and functional relationships in proteins. haemoglobin and lysozyme. 4. 5. parathyroid hormone. Proteolytic activation and protein sequencing.metabolic pathways. and Cox. (2000). Zymogen activation. NewDelhi. eicosanoids. co-factors and prosthetic groups. Unit III: Lipids Lipid digestion. transport and storage. protein folding and chaperons. Voet . Mayes. Antioxidant enzymes and its physiological role-SOD. triacyl glycerol. thyroid hormones. purification and quantification. Principles of Biochemistry Third Edition (Freeman Publishers). methods of protein extraction.glucagon. Reference Books 1. Regulation of carbohydrate metabolism-catabolism and anabolism in animals.Biochemistry – Volume 1 Biomolecules.androgens. P. (2005). Unit V: Hormone and its mechanism of action Structure. mineralocorticoids and Hormones that bind to cell surface receptors-FSH. glucose 6 phosphate dehydrogenase. mobilization. somatostatin. separation and structural analysis.Biochemistry by Rawn. Nelson. N. Granner. Rodwell. plasmalogen. Mechanisms of Enzyme Action. estrogens. Donald Voet and Judith G. Protein targeting in eukaryotes and prokaryotes. Prasad (2003). Characteristics and 3 D structure of enzymes. Unit IV: Enzymes and Co-enzymes Enzyme catalysis. Robert K. Conjugated proteins-Myoglobin. D. Chand publication 5.Panima Publications.phosphoinositides. Murray 2. Robert K. 3. Lipids with specific biological activities.G. glycerophospholipids and eicosanoids. luteinizing hormone. Text Books 1. separation.Box 20195 Kathmandu. A. A Text Book of Biotechnology by B. New York. M.

scale. recycle and continuous cultivation. air lift and loop reactor.M. Concepts of process control. Department of Biotechnology   11  . Shuler and Kargi. Unit. Second Indian Reprint (2004).V Process Control Online data analysis of physico chemical parameter measurements for biochemical processes. Text Books: 1. Prentice Hall. oxygen uptake rates and determination of oxygen transfer coefficients (kla). PIPIB Controllers. Unit . role of aeration and agitation in oxygen transfer. Bioprocess engineering. mass transfer in biological systems Unit. internal mass transfer in heterogenous reactions. TQ. Heat transfer processes in biological system. Unit. continuous.IV Process design and operation of Bioreactors Operational modes of reactors – batch. minimizing mass transfer effects. IQ protocols. heterogenous reactions in bioprocessing.up criteria for bioreactors. Outcome At the end of the course the student will be familiar in fermentation process and metabolic stoichiometry and the way to enhance the product of interest. packed – bed and hollow – fibre membrane bioreactors. novel bioreactors: Stirred tank.10BT302 ADVANCED BIOCHEMICAL & BIOPROCESS ENGINEERING Credit:4:0:0 Objective To acquire the knowledge about the various biochemical reactions and the ways to alter the biochemical pathways. reactors for waste – treatment processes. Thiele modules . enzyme deactivation kinetics. Unit I Transport phenomena in bioreactors Mass transfer in heterogeneous biochemical reaction system: Oxygen transfer in submerged fermentation processes. GLP.concentration gradients and reaction rates in solid catalysts. Fuzzy logic and neural networks in bioprocess control. mass and energy balances for steady state and unsteady state biochemical systems.solid – liquid mass transfer correlations. Case studies on bioreactor control problems.II Data Analysis in bioprocess Collection and analysis of data on bioprocess engineering. fed batch.M kinetics. GMP Practicers. Fluid flow and mixing. repetitive batch.III Reaction Kinetics in bioprocess Reaction kinetics for biological systems.Application of Artificial Intelligence.

McGraw Hill. Whitaker. unfired pressure vessels with internal pressure. Unit I: Engineering Properties Introduction to various mechanical properties of materials to be used as material of construction. Filtration Equipment and Crystallizers. and Ollis. Piping and Instrumentation Diagrams. reboilers & related equipment.E. Hall & A. Unit II: Storage Tanks Design of non-pressure storage vessel. 2.F. Biochemical Engineering and Biotechnology Handbook. Unit IV: Heat Transfer Equipments Detailed design and drawing of heat exchangers (shell & tube..Working stresses. fed batch. 4. 1993. Ltd. 1995. Peter F. Biochemical Engineering Fundamentals. Butterworth – Heinemann An Imprint of Elsevier India Pvt. resistance of metals to corrosion under varying conditions of temperature and pressure etc. Biotol Series. Factor of safety .2. continuous and airlift Fermentor. D. B. 2005 References: 1. tall vertical vessels. This course focus on plant layout and design of piping systems. Bailey J. Principles of Fermentation Technology. McGraw Hill (2nd Edition) (1993)..Lee . Bioprocess Engineering Principles – Pauline M Doran. & Mavituna . Stephen J. (1986). 10BT303 PROCESS EQUIPMENT DESIGN AND DRAWING Credit: 4:0:0 Objective: To design safe and dependable processing facilities in a cost effective manner. Biochemical Engineering by James M. Second Edition. 3. Bioreactor Design and Product Yield . Condensers. evaporator. Atkinson. Design of Centrifuge. exchanger). Academic Press. Unit III: Design of Fermentor & Separation Equipment Design of batch. Department of Biotechnology   12  . Butterworth – Heinemann. Application and use of various codes and standards in design. Outcome: At the end of the course the student will be familiar in design and engineering principles involved in process plant layout and piping design. Prentice Hall 1992 5. Design of unfired pressure vessels with external pressures. Stanbury. F.

2. Fabrication and Erection of Mild Steel Cylindrical Welded Oil Storage Tanks. (Published by Bureau of Indian Standards. Texas.1979: Specifications for formed ends for Tanks and Pressure vessels. dryers –rotary dryers and tray dryers and extractors. Peters Max. 10BT304 METABOLIC REGULATIONS AND ENGINEERING Credit: 4:0:0 Objectives The paper aims to give the students the different methods of metabolic regulations.E. (c) IS : 4179 . (e) IS : 4503 . 4. I. (2nd Ed. Text Books: 1. R. (f) IS : 803 . Gulf Publishing Company.Unit V: Mass Transfer Equipments Detailed design and drawing of Distillation columns.1969: Code for Unfired Pressure Vessels. Unit I: Introduction to Metabolic Engineering: Basic concepts of Metabolic Engineering – Overview of cellular metabolism: Metabolic flux integration of anabolism and catabolism. McGraw Hill (ISE).: Perry’s Chemical Engineers' Handbook. & Green.1968: Specifications for Shell Flanges for Vessels and Equipment. (2000).F.: Applied Process Design for Chemical & Petrochemical Plants. 1983). Ludwig. Indian Standard Codes: (a) IS : 2825 .1967: Specifications for Shell & Tube Heat Exchangers. Limiting accumulation of end products. 2. (2000). Gulf Publishing Company.and Ronald E West “Plant Design and Economics for Chemical Engineers”.H. Strain Department of Biotechnology   13  .1962: Code of practice for Design.W. E.). different methods of synthesis of primary and secondary methods and different methods of bioconversion that can be used in industry. 3. New Delhi. R. II & III.1967: Sizes of Process Vessels & their Leading Dimensions. New Delhi). Timmerhaus Klaus D. (b) IS : 4049 . Vols. (d) IS: 4864 to 4870 . (7th Ed. References: 1.: Process Equipment Design. S.). 2.: Random Packings & Packed Towers (Design & Application). (2000).. Texas. 1979. L.H.E. Strigle. Brownell. D. & Young. E. Wiley Eastern.2003 V Edition McGraw Hill. the students would have learnt about metabolic regulation and various methods of synthesis of primary and secondary metabolites and bioconversion. Perry. (1977. Outcome At the end of the course.

D. cephalosporin. W. RNA based metabolic regulationmicroRNA’s.Crueger. Zubay G. concerted feed back regulation. Lilly M. mixed or sequential bioconversions. Specificity...E. Vitamins (Vit B12. chemical bioprocess.pharmaceuticals. Dunnil.C. trophophaseidiophase relationship. organic acids (citric acids. gene dosage. Enzymes.L.. Vit A. acetic acid). lysine and phenylalanine). Regulation of RNA synthesis by amino acid. Narosa Publ. 1980. 2.. lactic acid. 3.. factors influencing growth cycle peak.protease. Biotechnology. Product inhibition. Stephen J.L. Factors affecting bioconversions. group translocation.P. Butterworth – Heinemann An Imprint of Elsevier India Pvt. Stanbury. recognizing growth cycle peak. Co metabolism.I. repressor mutants. Principles of Fermentation Technology. Hall & A.. " Fermentation And Enzyme Technology ". Alteration of feed back regulation. Metabolic pathways and regulation of production processes. “Biochemistry ". cumulative feed back regulation. agriculture. glutamic acid. amylase. Regulation of gene expression in eukaryotes. Vit B2.selection.A Text Book of Industrial Microbiology 2. regulation in branched pathways. Crueger and A. Riboflavin). precursor effects. Ltd. Unit II: Regulation of Gene Expression and Metabolic Pathways: Regulation of gene expression in prokaryotes -Lac operon. active transport. alcohol (Ethanol). Humphery A. 1989. Whitaker. Unit III: Regulation of Primary Metabolites and Enzymes: Producers of primary metabolites. Feedback regulation. Macmillan Publishers. Cooney C.. cellulase. Applications of Metabolic Engineering.antibiotics (penicillin.differential regulation by isoenzymes. John Wiley And Sons.amino acids (Tryptophan. Peter F. food technology. 2005 References Books: 1. House. permeability control: passive diffusion. erythromycin.. Second Edition. Demain A. Wang D.. applications of secondary metabolites. environmental bioremediation and biomass conversion. Steroids. Conversion of insoluble substances. Mycotoxins. 1999 Department of Biotechnology   14  . Unit IV: Regulation of Secondary Metabolites: Producers of secondary metabolites. lipase. Molecular Biology. David Friefelder. aspartic acid. Applications of Bioconversions in food industry. Mutants resistant to repression Unit V: Regulations of Bioconversions: Bioconversions. streptomycin). Yields. Text Books: 1. Genetic improvement of strains. agriculture. Metabolic pathways and regulation of production processes.

characterization and applications. chemiluminescence assay. T-cell activation parameters. Applications of ELISA and Radio Immuno Assay (RIA) in immunodiagnosis. ELISPOT assay. immunotherapy. 2004. Outcome At the end of the course would have learnt about the mechanisms by which a human body interacts with a pathogenic microbe & how it eliminates it. Unit II: Antibodies and Immunodiagnosis Recombinant monoclonal and polyclonal antibodies – their production. Unit I: Basics of Immunotechnology Antigens: Types of antigen.. Talwar G. Applications of cytotoxic T.10BT305 ADVANCED IMMUNOTECHNOLOGY Credit: 4:0:0 Objective This course aims to develop the skills of the students in Immunotechnology. Students. Unit IV: Immunopathology Preparation of storage of tissues. Text Books: 1. macrophages activation. and Gupta S. Vol. Western Blot analysis. isolation and characterization of cell types from inflammatory sites and infected tissues.K. phage display.. Unit V: Molecular Immunology Recombinant vaccines. immunodiagonosis. Department of Biotechnology   15  . 1 & 2. also familiarize themselves with immunopathology and immunotherapy.cell assay in cell mediated immune response. immunoenzymatic and immunoferrtin techniques for disease diagnosis. P. preparation of antigens for raising antibodies: peptide antigens and their preparation. Immunoeletrophoresis. production anti idiotypic antibodies. immunopathology etc. A Hand book of Practical and Clinical Immunology. application of PCR technology to produce antibodies. cytotoxicity assay. Immunohistochemistry. identification of antigens in tissues.cell assay. helper T. CBS Publications. viral coat proteins as recombinant adjuvants and their mode of action. adjuvants. Unit III: Techniques to assay Cell Mediated Immunity Identification of lymphocytes and their subsets in blood. application of recombinant DNA technology for the study of the immune system. estimation of cytokines. handling of animals. antigen antibody response. catalytic antibodies. functional studies on isolated cells. their structure. macrophage microbicidal assays.

6th Ed. and the effect of mode of administration upon program effectiveness. Department of Biotechnology   16  . 2009 2.2009. Unit III: Safety Performance Work environment-noise-effect of noise-unit of sound-noise levels in industries-control of noise-lighting-intensity of light for various operations in industry-measurement of intensity of light-industrial illumination-requirements for good lighting-industrial ventilation and exhaust systems.1988. Flixborough in UK. NewYork. UK. Unit IV: Accidents Identification and analysis of causes of injury to men and machineries-accident preventionaccident proneness-vocational guidance. 2009. Chakravarthy AK. in Bhopal (India). Portland Press Ltd. Outcome: At the end of the course the student will be familiar in safety program in terms of effectiveness to improve safe work practices and good housekeeping. 10BT306 INDUSTRIAL SAFETY ENGINEERING Credit: 3:0:0 Objectives: To develop highly qualified professional manpower the basic requirement lies on Systematic quality based coaching and training in Advanced Science and Technologies. Antibody Laboratory Manual by Ed Harlow. Pandian. Unit I: Introduction Major industrial accidents in India and in other countries-case studies of the accidents. Immunology & Immunotechnology. Unit V: Health Hazard Health hazard–occupational-industrial health hazards –health standards and rules-safe working environments. Immunotechnology.dangerous and toxic chemicals . Oxford University Publishers. Seveso in Italy.highly radioactive materials-safe handling and operation of materials and machineries-planning layout-safety slogans. Panima Publishers. 3. Cold Spring Harbor. Gosling JP. Reen DJ. Reference Books: 1.2. fire prevention and fire protection-personal protective equipments. 2nd Ed. Unit II: Industrial Safety High pressure-high temperature operation. Immunology and Immunotechnology. 2nd Ed.

DYNAMICS AND CONTROL Credit: 3:0:0 Objectives: The fundamental background for understanding and controlling the dynamics of chemical processes. Note: Industrial Visit: Report – Mandatory Text Book: 1.H. New York(2000) 10BT307 PROCESS INSTRUMENTATION.S.on site and off site safety provisions. Jaico Publishers. AITBS Publishers . principles of pneumatic and electronic controllers. New Delhi (2001) 2. R. Occupational Safety and Health Management. Safety Management in Industry.. Unit III: Frequency Response Department of Biotechnology   17  . McGraw Hill Co. New Delhi. Unit. first order systems and their transient response for standard input functions. Through it the students acquire the ability to apply mathematical and computational tools and engineering knowledge to formulate and solve process dynamics problems and to design appropriate control schemes to regulate the processes. Safety and Accident Prevention in Chemical Operations. Wood 4. linearization and its application in process control. Jain and Sunil S. servo and regulatory problems. Unit II: Closed Loop Systems Closed loop control systems. management and trade unions in promoting industrial safety. New Delhi (2006) Reference Books: 1. K. first order systems in series. development of blsock diagram for feed-back control systems. Grimaldi and Simonds . Safety Management. Anton. Khanna publishers .I: Open Loop Systems Open-loop systems. 3. Health and Environment Management Systems.Role of Government. H. safety organization. transportation lag. Fawcet and W. Outcome: At the end of the course the student will be familiar in process parameters and the effectiveness of controller designs. Krishnan. transient response of closed-loop control systems and their stability. Industrial Safety . Rao . second order systems and their dynamics. transfer function for controllers and final control element.

Unit. body weight body composition. Macro and micro Department of Biotechnology   18  . Unit II: Vitamins and Minerals (13) Outlines of vitamins and minerals.. cascade control. T. McGraw Hill. BMR.New York. 2000 10MB301 NUTRITIONAL BIOCHEMISTRY AND PHYSIOLOGY Credits: 4:0:0 Objective To impart the knowledge on Historical overview of Nutrition. introduction to computer control of Biochemical processes.V: Advanced Control Systems-II Control of distillation towers and heat exchangers. New York. 2003. feed forward control. and Corripio. stability criterion. 3. Ahuja Publishers.. the students would have learnt about the correlation between nutrition and physiology. New York. B. Smith. Prentice Hall of India. Outcome At the end of the course. overall metabolism of ingested nutrients. “Process Control “. 2. Stephanopoulos.S.. C. A. 2.. RDA. G. tuning of controller settings Unit IV: Advanced Control Systems -I Introduction to advanced control systems. factors influencing metabolism of nutrients and nutritional biochemistry. nutrients. model predictive control. “Process Dynamics and Control”. essential nutrients for metabolism. E. SDA and RQ. Physico chemical properties and physiological actions of Dietary fibre protein energy malnutrition. “Process Systems Analysis and Control “. structures. D. McGraw Hill. New Delhi. A. Dietary source. Macro and micro elements – Dietary source. functions and deficiency states.“Chemical Process Control “. control system design by frequency response techniques. 2000. 2000. Classification of vitamins – Fat soluble and water soluble. Unit I: Nutrition and its physiological role (12) Definition for nutrition. structures. Bode diagram. measurement of energy expenditure – calorimeter. Text Books: 1. 2nd Edn. Sundaram. “Principles and Practice of Automatic Process Control”. John Wiley. Coughnowr.Introduction to frequency response of closed-loop systems. 2002 References: 1. RDA. Marlin. functions and deficiency ststes.

Excretory system – structure of nephron.elements_ Dietary sources. Calcium. introduction to systemic anatomy – types of bone – joints – classification of joints – innervations of joints – muscle tissue and muscular system – types of muscle – blood vessels and cardiovascular system – lymph and lymphatic system – functions of the lymphatics. Martin Eastwood. formation of urine. diseases associated with liver. visual auditory pathway. structures. Department of Biotechnology   19  . Out Come: Students would learn the metabolism of biomolecules and their metabolic relationships (integrated metabolism). acidosis and alkalosis. Text Book: 1. Reference Book: 1. kidney. obesity. Unit III: Metabolic Disorders and its Dietary Management (13) Dietary management with special reference to Diabetes mellitus. Cardiovascular diseases. infants children and adolescent. tubular reabsorption and secretion: Nervous system – organization. XI edition (1994). Dietary requirement in pregnancy. ‘Human Physiology’ Volume I. lipids. proteins etc. Respiratory system components and their function – transport of oxygen and carbondioxide. lactation. transfer of blood gases. Unit V: Circulatory system and its physiological role (15) Circulatory system – composition and functions of plasma. action potential. Integrate the various aspects of metabolism & their regulatory pathways. RDA function and deficiency of Iron.’ Principles of Human Nutrition’ Blackwell publishing. Unit IV: Anatomy (12) Overview of Anatomy – medical and anatomical terminology – sections of the body – anatomical variations – diagnostic imaging. 10MB302 ADVANCED INTERMEDIARY METABOLISM Credits: 4:0:0 Objective: To learn the metabolism and integration of biomolecules that takes place in human system. Water and electrolyte balance and regulation. phosphorus and magnesium. Iodine. blood coagulation. nerve impulses and neurotransmission. Medical Allied Agency.. II edition (2003). Chandi Charan Chatterjee. Physiology of pregnancy and lactation. Acid base balance: regulation. intestine (Steatorhoea) and Cancer. Digestive system – composition and functions: Digestion and absorption of carbohydrates. zinc and copper. Structure and functions of Reproductive system. gastric (peptic ulcer).

and hypouricemias. energy production & regulation. enzymes. Cellular metabolism of lipids as a basic source of energy.reactions. fructosuria. glycogenic and ketogenic amino acids. NH3 formation . Glycolysis in erythrocytes. localization and main steps.replication and transcription of DNA. Specificity of metabolism of the RBC. enzymes. b-oxidation . coupling of glycolysis with other pathways. Inhibitors of proteosynthesis. oxidation of FA and ATP production. decarboxylation. galactosemia.Bile acids and salts.atherosclerosis Unit III: Metabolism Of Proteins. Synthesis of vitamin D from cholesterol.reactions. absorption and transport. metabolic control.Metabolic changes during starvation. Regulation of nucleotide production. Unit IV: Metabolism Of Nucleic Acids Metabolism of nucleotides . bypasses. major functions.Unit I: Metabolism Of Carbohydrates Carbohydrate metabolism . And Amino Acids Nitrogen balance (positive. coenzymes. synthesis of deoxyribonucleotides. Inhibitors of purine and pyrimidine biosynthesis. Disorders in glucose metabolism. Gluconeogenesis . metabolism of aminosugars. biosynthesis of unsaturated FA Metabolism of prostaglandins . metabolite. Biosynthesis and degradation of heme and molecular disorders (porphyria. ketogenesis and ketones bodies Biosynthesis of FA. oxidative deamination. Lesh-Nyhan syndrome. glycolysis .. Synthesis of nonessential amino acids. Pentose phosphate pathway . Uronic acid pathway . Biosynthesis of cholesterol and its role in organism. FA synthase complex. elongation of FA chain. nonessential). catabolic reaction of purines and pyrimidines . lipoproteins. disorders in fatty acids metabolism. Xenobiotics Department of Biotechnology   20  .purine and pyrimidine synthesis. metabolism of galactose and fructose. intermediates. coenzymes. mRNA.lycogenesis and glycogenolysis. biosynthesis of tRNA.. the role of malonyl-CoA and acetyl-CoA. enzymes and coenzymes in glycolysis.general view. mobilization of stored fat.pentosuria. rRNA. Macro & micro – elements & their role in health & disease. disorders in amino acid metabolism. Nucleic acids and proteosynthesis . water metabolism & its regulation. fatty acid oxidation . reducing equivalents (NADPH) in biosynthesis. steroid hormones biosynthesis.the role and function. hyperbilirubinemia . lipid myopatia. amino acids (essential. enzymes. hyper. ketonuria. negative). intracellular degradation of proteins. Salvage pathways.metabolic disorders (gout. Unit V: Metabolic Interrelationships & Regulatory Mechanisms Metabolism of Hemoglobin. glycogenesis Unit II: Metabolism Of Lipids The role of lipids in the organism. ketonemia.).steps. urolithiasis). Metabolism of glycogen .localization. Metabolic transformation of individual amino acids. regulation of the processes.reactions. b-oxidation of odd numbered FA and unsaturated FA. Fat digestion. alcoholic fermentation.the urea cycle reactions. catabolism of carbon skeletons of amino acids. transamination. regulation and inhibition of proteosynthesis.

or other therapy is likely to be the most appropriate response. To learn the scientific approaches that are used to investigate these processes such as how do microbial pathogens modulate host cells to expedite virulence. D. 2003. and distinguish when a vaccine. treatment and control measures of diseases caused by Staphylococcus aureus. 2) Textbook Of Medical Biochemistry .M.K. Infection and infectious process routes of transmission of microbes in the body.Rawn.L. Biochemical reactions. John Willey Sons. antibiotic. VI edition. P.Stumph.W. Text Books: 1) Harpers Biochemistry Ed. Lehninger . 3) Biochemistry. host-microbe interactions.H.E. Streptococcus. Murray . Mycobacterium tuberculosis. J. 2003. Corynebacterium diphtheria. 2007. Haemophilus influenza.H. 2004.K. Antigenic structure. mode of transmission.Perry.E. G. Enterobacteiaceae. 2009. R. D. The Mc Graw-Hill Company.J. Clostridium tetani.Bruening and R.Moran and G. Treponema pallidum. Unit I: Bacteriology Normal microbial flora of human body. Reference books: 1) Biochemistry. Inc. Prent Hall. Proteus. L. types of basic biotransformation reactions. Freeman and Co. Pseudomonas aeruginosa.Voet.A.A. 2006. VI edition.Doi. Nelson & Cox .R.N Chatterjee.Horton. V edition. D.M. pathogenicity.E. Bacillus anthrax.K.Stryer.Scrimgeour.Conn. 6th Edition.D. Granner. 2) 2)Principles of Biochemistry .and their biotransformations. Ed. and the mechanisms of pathogenesis.Voet and J. Also to compare and contrast therapeutic treatments for microbial infections. Vibrio cholera. Salmonella typhi.Rodwell. P. Lab diagnosis. Mayes &V. Cultural characteristics. Chlamydia and Mycoplasma Department of Biotechnology   21  . 4) Principles of Biochemistry.Tymocko and L. 5) Out lines of Biochemistry. VI edition. III edition. 10MB303 MEDICAL MICROBIOLOGY Credits: 4:0:0 Objectives: This course will focus on mechanisms of microbial pathogenesis and the host response. Rana Shinde. Klebsiella pneumoniae.Berg.coli. Jaypee publishers. how do host cells respond to microbial pathogens Outcome: At the end of this course students will be able to compare and contrast different microbial diseases.CBS publishers & distributers. 2006.G. the role of liver cells in detoxification of xenobiotics. Shigella. John Willey and Sons. including the properties of different types of pathogens.

Helminthes.. Ananthanarayan. herpes virus (Chicken Poxzoster). Text Book of Microbiology. viruses affecting nervous system. HIV virus. 2007. 2005. Sulfonamides. Department of Biotechnology   22  . Pidia Unit -III: Parasitology Parasitology – Morphology. R. 2004 3. Academic Press. Emerging viral diseases like Chicken Guinea virus. Systemic Mycosis – Histoplasmosis. Mode of antibiotics: Penicillin. Problems of drug resistance and drug sensitivity. Biochemical reactions. Elsevier-Mosby Reference Books 1. reovirus.Unit II: Medical Mycology Medical Mycology – Culture characteristics. Medical Microbiology. Fenner. and Polymyxins Antifungal drugs (Nystatin). treatment and control measures of diseases caused by Cutaneousmycosis – Dermatophytosis. Lehman and George Manuselis. Induction of interferon. Rosenthal. opportunistic mycosis – Candidiasis. viral hepatitis. All India Publishers & Distributors. human adenovirus. types of inducers. adenovirus. other respiratory viruses. Donald C. 1995. 6th edit. Antiviral agents . Text Book of Medical Mycology. P. lytic and lysogenic cycle. lambda phage. Medical Virology. Mode of transmission. enterovirus. Subcutaneousmycosis – mycetoma. Oncogenic viruses (tumor viruses) DNA containing oncogenic viruses.A. Superficial mycosis – Tinea. Antigenic structure. H1N1 virus and Japanese Encephalitis virus Text Books 1. 1994. And Paniker C. X 174 phages. pathogenicity. Lab diagnosis. treatment and control measures of diseases caused by Coccidian – Toxoplasma. Lab diagnosis. 2004 2.chemical and biological agents . taenia solium.R. Antiviral agents. Unit V: Virology (12) Viral diseases: Description and pathology of diseases caused by pox viruses. Ascaris lumbricoides. 3rd edition. Trypanasoma. pathogenicity.K. Drug resistance in bacteria. Amoebic dysentery. Interprint / Mehta Publishers. White David O. Principles of chemotherapy. Bacterial phages & viruses . Jagdish Chander. retroviruses (Onco RNA viruses). Textbook of Diagnostic Microbiology. Mode of action of anti. by Connie R. Subash Chandra Parija. types and classification.S. myxo and paramyxo viruses. AIDS virus.protozoan agents.Interferon Nomenclature. Murray.Structure and replication of RNA and DNA bacteriophages. Pfaller. Dengue virus. Antigenic structure. 2. 4. Orient Longman. and M. Giardiasis. RNA containing oncogenic viruses. infection on host. Fasiola hepatica. Textbook of Medical Parasitology. Cryptosporidium. Mahon. Streptomycin. K. Unit IV: Molecular Virology Animal viruses. Mode of transmission. Hepatitis viruses.

for which genomic or proteomic testing or therapies could then be applied for confirmation and/or treatment.Internet – facilities used in the internet web browsers STTP 5. Text Book Department of Biotechnology   23  . Unit I: Biomedical Systems Coding -Common health care language .HTTP. and to characterize those resources in terms of search and display functionality. Unit V: Clinical Data Analysis (11) Alignment techniques – BLAST.10MB304 MEDICAL BIOINFORMATICS Credits: 3:0:0 Objective: The objectives of this study are to determine.comparison of two sequences– identity and similarity – global and global similarity – global and local alignment.Biological data basics – primary secondary data basics – Protein pattern data basics – DNA sequences data basics . Impact of chromosomes on genes.P Data base searching . Protein information resources .coding techniques – coded and quasi coded data Medical vocabulary – industry wide communication standards HL7 – unified medical language system – quality of care paradigms.multiple sequence alignment – data basis of multiple alignment – secondary data base. Unit IV: Cell And Molecular Bioinformatics Basic Genetic Science : Study of cell. CLUSTA. nucleus. URL – European molecular biology network – national centre for biotechnology information.integration of decision support in clinical processors. risk management bioethics. elements that are common across a range of bio-health information resources. and to better understand. gene study.DNA analysis . HTML. These models may point to an underlying genomic or proteomic cause. Expert system-Principles of expert system – phylogenetic tree construction .Electronic patient record – models or ERP –environmental services – metrics – telemedicine – community networks –telemedicine peripherals and equipment selection – anatomy of video conferencing technology. Outcome: Students will learn the computer-generated models that indicate the presence of a disease or the relative impact of a particular treatment. Unit III: Bioclinical Transcription Patient record maintenance .Genes structure and DNA sequences – interpretation of EST structures – different approach to EST analysis. chromosomes and their components Evaluation of chromosomes. N. Unit II: Biological Information Information networks . Our ultimate goal is to better define the role of bio-health information in clinical practice and in biological research.

evolution. yeast. 2. structure. electrochemical protein gradient. metabolic exchange by chloroplast membrane.elegans. carbohydrates. including gene expression. lysosomes and protein sorting and transporting. light absorption and conversion of CO2 to carbohydrate. Unit IV: Functional Aspects of Cell Division Nucleus. eukaryotes. chromosome structure and dynamics. control of cell division.. cell fractionation. SpringerVerlag". signal transduction. Experimental models: E.1. structure. T. carbon fixation. Nuclear envelope. Attwood. Coiera E. G2. cell growth and division. 1999 Reference Books 1. Bernser. slime mold. respiratory chain. endocytosis.K.coli. 10MB305 MOLECULAR CELL BIOLOGY Credits: 3:0:0 Objectives: Molecular and Cellular Biology (MCB) to be an authoritative source of fundamental knowledge and new developments in all aspects of the molecular biology of eukaryotic cells and prokaryotic cells. The internet and telemedicine. Chajsman & Hall medical". continuous and synchronous cell culture. chemosmotic theory. cytosol. Drosophila and Arabidopsis thaliana. Outcome: Students can understand about the microbial as well as higher organisms and on viral systems where the emphasis is clearly on the cell. Pearson Education. Single cell to multicellular organisms. D. events in phases: G1. New York. Michael L.. 1999. M. Cytoskeleton and cell movement. "Fundamental Concepts of Bioinformatics". rough and smooth endoplasmic reticulum. electron transport chain.J. cell cycle. E. Raymer. " Clinical decision support systems. intracellular trafficking. 3. transport of small and macro molecules-ectocytosis. membrane proteins. Theory and practice. Golgi. Unit II: Cellmembrane and Transport System Molecular organization of cell: plasma membrane. and mammalian genetic models. Pearson Education. "Guide to medical informatics. lipid bilayer. C. Unit I: Basics and Functions of the Cells Cell structure of archae and eubacteria. Krane . Unit III: Metabolisms of Cell Organelles Mitochondria-origin.S. chloroplast: origin and evolution. Parry-Smith. 2002. "Introduction to Bioinformatics". redox potential. Dan E. ribosomes. London 1997. Department of Biotechnology   24  . S.

EEG. (2nd edition). bioelectric signals and their recording. Washington. 8th edition. characteristics and selection of transducers for biomedical instrumentation. DeRobertis EDP and DeRobertis EMF Jr. Genes VIII. Bioelectric signals ( ECG. electrode tissue interface. (2004) Cell and Molecular Biology. Lewin B (2004). Philadelphia.Unit V: Cell Cycle and Cancer Regulations Concept of oncogenes. USA 2. tumour suppressor genes and their role in cell cycle. Transducers for biomedical application. Si RNA and its role in gene therapy. Diagnostic. bioelectrode – types. Reference Books 1. ASM Press. contact impedance. neoplastic cell growth. 10MB306 NUTRITIONAL BIOCHEMISTRY AND PHYSIOLOGY LAB Credits: 0:0:2 12 Experiments will be notified by the HOD from Time to Time. Types. EMG and EEG) and their characteristics. 10MB308 BIOMEDICAL INSTRUMENTATION Credits: 3:0:0 Objective: To learn about the various medical instruments and latest techniques used in the hospital for diagnostic purpose Outcome: Students are expected to be thorough with latest biomedical instruments and their handling techniques Unit I: Biomedical Equipments Classification of biomedical equipment. New Jersey. EMG. Cooper GM (2000) The Cell: A Molecular Approach. USA. USA. Text Book 1. Lippincott Williams & Wilkins. Unit II: Therapeutic Components Department of Biotechnology   25  . Pearson Prentice Hall. electrodes for ECG. 10MB307 MICROBIOLOGY LAB Credits: 0:0:2 12 Experiments will be notified by the HOD from Time to Time. therapeutic and clinical equipment.

Autoanalyser – types and application. angiography.Biosensor – Mechanism and types. CO2 laser. Biomedical Instrumentation by Cromwell. argon laser and helium neon laser. Identify biochemical diagnostic agents or tests useful in: diagnosis and monitoring response to therapy. classification. Physics of ultra sonic waves. Handbook of medical instruments by R. life time. Outcome At the end of the course students will be well versed in the medical terminology. MRI scan and echocardiography.2007 2. Text books: 1.S.ray imaging. Characteristics. 2009 Reference Books: 1. medical ultrasound. therapeutic application of endoscope.YAG scan. techniques and acquire knowledge on diseases and disorders. Department of Biotechnology   26  . Unit V: Therapeutic Instruments Therapeutic instruments – Introduction. merits. demerits. computer tomography and linear tomography. ND. power source and electrodes of cardiac pacemaker and defibrillators. Unit IV: Laser Techniques Introduction. Laproscope and cardioscope. metabolic functions. Introduction. B scan. 2002. types. 2. diagnostic. Basic NMR components and its application in medicine. 2001 10MB309 ADVANCED CLINICAL BIOCHEMISTRY Credits: 4:0:0 Objectives To interpret biochemical diagnostic assays. CT scan. limitations. Principle and application of Sphygmomanometer. Conventional X. different mode of operation of ultrasound – A scan.Khandpur. disease state. Principles of applied Biomedical instrumentation by Goddes and Baker –John Wiley.Computer application in medicine – computerized catheterization laboratory – computerized patient monitoring system. Unit III: Scanning Techniques X-ray machine. fluoroscopy. radiography. Automatic tissue processing and application of microtome. Biomedical instrumentation and measurement by Carr and Brown Pearson. diagnostics and therapeutic application and advantage of pulsed ray laser. application of ultra sound scan. Ultra sonic imaging system. types. Application of surgical diathermy equipment and haemodialysis in medicine. Pulse oximetry – introduction. Magnetic resonance imaging system.

immunological test for pregnancy. and clinical significance of transaminases. amylase. isocitrate dehydrogenase. fatty liver and gall stones.reactive protein test.Unit I: Specimen Collection and Processing Collection of blood vein puncture. Radioisotopes & their clinical applications. prothrombin test. Enzyme pattern in diseases:. Hemoglobinopathies. chymotrypsin.chemical examination and infections. Free radicals in diseases:. Colorectal cance. Glomerular disease. Amniotic fluid:. Lesh Nyham syndrome. Types of free radicals. Porphyrins and porphyrias. phosphatases. ESR.Introduction . Clinical significance of urinary components with reference to sugars. leukemia. skin puncture. erythrocyte enzyme disorders. Unit V: Cancer Biology Oncology:. collection. Catalase. 5’nuclotidase. 1994 Department of Biotechnology   27  . creatine kinase. breast cancer and gastrointestinal tract cancer. Gastric and pancreatic function test:. Prostate cancer. W. Galactosemia. albinism. Thalasemia. glutamate dehydrogenase. alkaptonuria. Unit IV: Organs Associated with Disease Diagnosis Liver function test and related disorders:-Jaundice. composition and analysis of amniotic fluid.. Renal function test and related disorder:. choline esterase. Coagulation test. tubular diseases.Saunders company. Principle. Hemoglobin: Normal and abnormal Hb.Myocardial infarction. cirrhosis. hepatitis. GPX and GSHs. Niemann Pick’s diseae. hepatobiliary diseases. Alpha fetoproteins. Text Books: 1. Rhumatoid arthritis (RA) test. carcino embryonic antigens. proteins. Hunters syndrome. gamma –glutamyl transferase. Erythrocyte metabolic pathways. assay. spinal cord infections. Fundamentals of clinical chemistry – Teitz. Collection of urine:-Timed urine specimens. Disorder of erythrocyte metabolic pathways. Enzymes in inborn errors of metabolism – Phenyl ketonuria. Unit III: Clinical Enzymology Principles of Diagnostic Enzymology Factors affecting enzyme levels in blood.Composition and collection .B. throsinosis.Hyper and hypo lipoproteinemias and diagnostic test for lipoprotein disorders. trypsin.Acute renal failiure. Seperation of hemoglobin. urinary tract obstruction. Hartnups disease. urine preservatives.Origin. Test for urinary compounds. Taysacch’s disease. collection with syringe. bilirubin and porphyrins. CSF:.Cancer markers for oral cancer. collection with evacuated tube. Unit II: Serology and Hematology C. analysis of urinary calculi. glucose -6-phosphate dehydrogenase and ceruloplasmin. lipase. Free radical induced lipid peroxidation and antioxidant enzymes – SOD. arterial puncture and anticoagulants. lactate Dehydrogenase. ketone bodies.

control of hypothalamic hormone secretion.Synthesis and chemistry of hormones. Cytosolic hormone receptors: Eicosonoids and hormone action. hematopoietic growth factors.second messengers. Clinical chemistry – principles. Ltd. Clinical biochemistry – Metabolic and clinical aspects.structure. hormones of the pituitary. its specific secretions and also the disorder and pathophysiology. Hormone bioassay-RIA. Growth hormones: somatotropins and somatomedins. circulation and metabolism. pathophysiology. Feed back mechanisms. pathophysiology. Unit Iii : Glands and Hormone Synthesis Thyroid gland:. Unit I: Basics Of Endocrinology Hormones. chemical structure. Marshall. Pathophysiology. Hormone secretion. William Heinmann medical books. hypophysiotropic hormones. Teitz text book of clinical biochemistry 3rd edition – Burtis et al. Transport and clearance. Pathophysiology.. vasopressin.2. Pearson Professional Ltd. Neuroendocrine integration in homeostasis. 5th edition – Varley et. Parathyroid gland: synthesis. epidermal growth factor. Feed back control of secretion .Introduction. Neurohypophysis: Synthesis and chemistry of N. chemistry and metabolism of parathyroid gland hormones . mechanism of action. hormone. physiological roles.1995 4. Endocrine hypothalamus. associated diseases and the diagnostic evaluation. 2000 10MB310 ADVANCED ENDOCRINOLOGY Credits: 4:0:0 Objective: The aim of the course is to provide knowledge on the endocrine system. 1999 3. control of thyroid hormone secretion. Bishop. Clinical chemistry in diagnosis and treatment 6th edition – Mayne. Roles and mechanism of action of oxytocin.al.Mechanisim of hormone action –receptors . 1980. Practical clinical biochemistry. mechanism of action. Hormones and homeostasis.. procedures and correlations.. ELISA. References 1. 2004 5. Growth factors: neurotropic growth factors. control of neurohypophyseal hormone secretion. Physiological role and vitamin D. Classes of chemical messeCngers. Lipppincott. CBS Publishers. calmodulin. Unit Ii: Hormones and Growth Factors Pituitary hormones. volume I and II. 1994 2.Anatomy of pituitary gland. Outcome: At the end of the semester the students would have learnt about the endocrine regulation.control of secretion. ELBS Publications. Clinical chemistry 5th edition – Mosby. Mechanism of action of calcium Department of Biotechnology   28  . Hormones and behavior.H.

Neurohormones:. Gain of Department of Biotechnology   29  . control of secretion. Unit Iv: Endocrine Secretions and Neurohormones Pancreas:. William’s Textbook of endocrinology 8th edition – Wilson.(2009). synthesis. 2. physiological roles. Functional cloning – eg.source. Smith et al. Endocrinology of pregnancy.endorphins-source. pathophysiology. Foster (2002) 10MB311 MEDICAL BIOTECHNOLOGY Credits: 3:0:0 Objective: To develop skills of the students in the field of medical biotechnology and its applications in various fields like diagnostics and therapeutics. physiological roles and mechanim of action. This will serve as a tool to understand the concepts in medical biotechnology. Catecholamines .Ovarian steroid hormone synthesis. melatonin secretion and circulation. 4th Ed. gene therapy and therapeutic products in medicine. Unit:I Classification of Genetic Diseases Chromosomal disorders – Numerical disorders e. Basic Medical Endocrinology. Chromosomal instability syndromes. deletions. Academic Press. Gene controlled diseases – Autosomal and X-linked disorders. sex differentiation and development.. insulin. Unit V: Reproductive Endocrinology Male reproductive system:.4thEd.Pineal gland . References: 1. diagnosis. proposed role of pineal. Melanotropic hormones.g. Pancreatic peptide – chemistry.chemistry.Endocrine pancreas. Goodman HM. chemistry and metabolism. role of MSH and mechanism of action. Identification of disease genes. Structural disorders e. pathophysiology. Female reproductive system:. Pathophysiology. the students would have learnt about genetic diseases. Molecular basis of human diseases .homeostasis. puberty and hormone control. Pathophysiology. therapy and treatment. glucagons. Principles of biochemistry. Mechanism of action and pathophysiology. Robert F.synthesis. mechanism of action. Text Books: 1. parturition and lactation. haemophilia gene . translocations & inversions. physiological role. Human infertility – reasons. 7th edition –McGraw Hill (2003) 2. Dons. chemistry. Physiological roles and mechanism of action. (2008). trisomies & monosomies. chemistry and metabolism of androgens. somatostatin.CRCPress. duplications.Endocrine and Metabolic Disorders: Clinical Lab Testing Manual.Pathogenic mutations. Neuroendocrine control of organ function. Outcome: At the end of the course.g.melatonin hypothesis. Mitochondrial disorders and Multifactorial conditions.

Medical Biotechnology.CD 4 receptor. maternal serum and fetal cells in maternal blood. Gene therapy trials – Cystic Fibrosis. Attenuated Vaccines– Cholera. Growth Hormone/Somatostatin. Vectors used in gene therapy Biological vectors – retrovirus. receptor mediated gene transfer. Genomic Imprinting -Mechanisms. Unit II : Diagnostics Prenatal diagnosis . Non-invasive techniques . Nanomedicine and Nanosurgery – for cancers. Loss of function . essentials of osteopathic principles. nanorobotics. Hypothyroidism. Elsevier Health Sciences. drug delivery. human genome.C.I Clinical management and Metabolic manipulation – PKU. Medical Biotechnology.ISBN Reference Books 1. Synthetic vectors– liposomes. Yuan Kun Lee Editor Yuan Kun LeeEdition2. 2009 3. Artificial Cells. Mary Keen Editors Judit Pongracz.Erythropoietin. Mary Keen Edition illustrated. monoclonal antibodies.Ex-vivo.DNA/RNA based diagnosis Hepatitis. Interferon. 2005 2. HIV . United Nations University Press. Familial Hypercholesterolemia. production of therapeutical monoclonal antibodies and their uses. Insitu gene therapy.Invasive techniques . Nanodevices.Vector vaccines – Cholera and Salmonella Text Book 1.II Conventional medical biotechnology.. detection of gene mutations. Fetoscopy. Microbial biotechnology: principles and applications. Gene therapy .genomic and c DNA arrays. Diagnosis using protein and enzyme markers. Invivo.Embryonic and adult Stem Cells. DNA based vaccines. role of organ transplantation in curing and new organ production..Ultrasonography. the principles and practices of transfusion techniques. neurological disorders. Medical biotechnology: achievements. Rickets.For Haemophilia. Unit V: Gene Products in Medicine Functional cloning – anti-haemophilic factor . Huntingtons Disease. 2008. Microarray technology.Amniocentesis. Judit Pongracz. tissue engineering and transplantation. Ribozymes. Positional cloning. P. 2006 Department of Biotechnology   30  . CFTR Pro drug therapy/ Antisense therapy. X-ray. Phenyl Ketonuria. illustrated. Pointer Publishers. Encapsulation technology and therapeuticsDiabetes. subunit vaccines – Herpes Simplex Virus.function mutations: Oncogenes.Trivedi. Diabetes Stem cell therapy . Albert Sasson. Bioartificial organs. World Scientific. Potential use of stem cells – Cell based therapies– Nanoparticles.Tumour Suppressor Genes. contemporary issues in medical biotechnology. Gene products in medicine – Humulin. Unit IV: Therapeutics . human and veterinary osteopathy and bone grafting. Congenital hypothyroidism. Beckwith Weidemann Syndrome. prospects and perceptions. preparation of blood components and their use. tPA. ADA. use of transgenic animals in therapy. application to diseases Unit III : Therapeutics .Dystrophin.

Diagnostic importance. Historical Background. stem cell therapy. safety guidelines of recombinant DNA research. RAPD.Basic principles and general requirements of patent lawbiotechnological inventions and patent law-Legal development-Patentable subjects and protection in biotechnology-The patenting living organisms. restriction and modifying enzymes. embryo transfer technology. Unit II : DNA Manipulation Techniques Inverse PCR. Taqman assay. mutation.Sanger’s method. Screening of libraries with DNA probes and with antisera Unit III Applications of rDNA and Bioethics Cloning in plants. prokaryotic and eukaryotic expression vectors.International conventions patents and methods of application of patents-Legal implications-Biodiversity and farmer rights. methods of nucleic acid sequencing. Primary Containment for Biohazards. To impart knowledge on moral issues. gene-therapy. (Kunkel’s Method). vaccines.Restriction mapping. Characteristics of plasmid and phage vectors. Nested PCR. genetic elements that control gene expression. Recombinant cytokines and antibodies. r-DNA technology. Introduction to Biological Safety Cabinets. Unit – V Biosafety Introduction. Biosafety Levels.ethical concerns of biotechnology research and innovation Unit – IV IPR and PATENTS Intellectual property rights -TRIP. Biosafety Levels of Specific Department of Biotechnology   31  . Yeast and Mammalian vectors.GATT. • Moral and Social Values and Loyalty • The rights of others Unit I : Basics of Recombinant DNA Technology Role of genes within cells. PCR and other applications of genetic engineering also • Engineering Ethics and Human Values. Outcome At the end of the semester the students would have learnt about r-DNA technology. design of linkers and adaptors. Site Directed Mutagenesis. transgenic and knockout animals. genomic library. Construction of cDNA and genomic libraries. RACE PCR. What is bioethics? The legal and socioeconomic impacts of biotechnology-Public education of the process of biotechnology involved in generating new forms of life for informed decision-making .10MB312 GENETIC ENGINEERING AND MEDICAL ETHICS Credits: 3:0:0 Objective Helps the student to understand and apply this knowledge in research to study the molecular mechanism of DNA and RNA synthesis and Protein synthesis. Molecular beacons. Invitro fertilization. PATENTS: Objectives of the patent system . Insect. IPR and biosafety.

Sasson A. 3. 1988. Reference books 1. An introduction”.K. "Current Protocols In Molecular Biology ". Risk Analysis. Sasson A. Biosafety guidelines . Text Book 1. Greene Publishing Associates. F. 10MB313 CLINICAL BIOCHEMISTRY AND EXPERIMENTAL HEMATOLOGY LAB Credits: 0:0:2 12 Experiments will be notified by the HOD from Time to Time 10MB314 ENDOCRINOLOGY LAB Credits: 0:0:2 12 Experiments will be notified by the HOD from Time to Time 10MB315 IMMUNOLOGY AND IMMUNOTECHNOLOGY Credits: 4:0:0 Objective: To enable the students to acquire knowledge in the field of infectious diseases and interaction with the host’s immune system. Sambrook et al Vol. 2006. Kingston RE. 1993. IV edition. “Gene Cloning.Government of India. Academic Press. UNESCO Publications. for GMO applications in food and agriculture. Recommended Biosafety Levels for Infectious Agents and Infected Animals. Overview of National Regulations and relevant International Agreements including Cartegana Protocol. Sixth edition. Primrose SB. also various mechanisms by which our immune system protects our body from microbial pathogens. RCGM. T. Crespi. 2001. Risk management and communication.. Roles of Institutional Biosafety Committee.S. Old RW. Brent R. Blackwell Science Publications. Berger Sl.Microorganisms. 2001. R. and Straus. GEAC etc. Environmental release of GMOs. An Introduction To Genetic Engineering ". Moore DD. Beier. Kimmer AR. 1987 3. Vol 152. 2. 5."Biotechnologies in developing countries present and future". Risk Assessment. “Molecular Cloning”. 4.2002. “Biotechnologies and Development ". Ansubel FM. Department of Biotechnology   32  . NY. Biotechnology and Patent protection-Oxford and IBH Publishing Co. "Principles Of Gene Manipulation. 1988. "Methods In Enzymology". Definition of GMOs & LMOs. 2. Black well publications. 1-3. UNESCO Publishers. New Delhi.

AIDS: Structure of HIV. MHC: Organization. recombinant vector vaccines. therapy and bone-marrow transplants. DNA vaccines. Transplantation immunology: clinical manifestation. RIA. antibody diversity. Monoclonal Antibodies. mechanism for induction of autoimmunity. Also can learn the modern techniques in immunology Unit I: Cells Of Immune System Experimental Animal Models: inbred strains.antibody interaction invivo -. Null cells. III & IV. Therapy. gene rearrangements in immunoglobulins.cross reactivity Antigen . animal models. immunological symptoms of AIDS. adjuant technology. Cytokines related diseases. multivalant sub-units vaccines. SCID mice. Cell mediated immunity: CTL mediated cytotoxicity. MHC molecules and genes. Cellular distribution. Antigen processing and presentation. ELISA . tumor evasion. Cancer immuno therapy. nude. whole organism vaccines. 2000 2. Unit II : Antibody Diversity and Therapeutics Antigens : B cell epitopes.precipitants. T cell epitopes. destruction of T cells. agglutinants. Cells of the immune system: haematopoiesis. Cancer immunology: Tumor antigens.Multigene family. NK cell mediated toxicity. Haptens : viral and bacterial antigens. complement deficiencies.antibody interactions invivo . Biological consequence of complement activation. Cytokines: IL. CSF. Immunoglobulins superfamily. Antigen . Immunoglobulin genes . proliferation & differentiation Unit IV: Hypersensitivity Reactions Hypersensitivity reactions .Paul Travels Department of Biotechnology   33  . Synthetic peptide vaccines. expression of immunoglobin genes. factor influencing immunogencity. Immunogloblins: domains classes and biological active antigenic determinants on lmmunoglobins. II. Cytokine. granulocytes adhesion molecules. Immunodeficiency diseases. gene therapy for treatment. Regulation of haematorpoiesis. Cytokine antagonists. Immunology V-The immune system in health and disease.Type I. delayed type hypersensitivity. Unit V: Autoimmunity Autoimmunity: Autoimmune disease in human. Immunological tolerance. Janeway Jr.Outcome: Students can understand how much the immune system important to the humans. B&T cell maturation. T cell membrane molecules. Unit III : Complement Complement Activation: Pathways regulation of complement system. Kuby immunology 4th edition – Goldsby et al. receptors. T-cell receptors. AIDS vaccine. hemolytic plaque assay. MHC and disease. knockout mice. regulation of MHC and immune responsiveness. TNF. Freeman and Co. haematopoietic growth factors. clinical uses of stem cells. Lymphoid cells – Lymphoblasts CD antigens.. Hypersensitivity disease. organ-transplants. activation. Text books: 1. immune response to tumors. Vaccines: Active and passive immunization.techniques and applications. IFN.

Hepato-biliary system. Virella. WBC disorders: Leukopenia. Immunology 3rd edition – Roitt et al. 2007 4. Leukocytosis. inflammation. clinical and chemical pathology.. aging and degeneration. Atopic and Anaphylactic reactions. 2002 10MB316 CLINICAL PATHOLOGY Credits: 4:0:0 Objective: The aim of the course is to provide basic knowledge on clinical pathology. Lung and Pleura : Effects of other systemic disorders. 2008 3. pathogenesis of tumours and tumour like lesions and their diagnosis. delayed hypersensitivity granulomatous reactions. Immunology 3rd edition Tizard.. Unit IV: Immunopathology Immune responses. Marcel Dekker Ltd. Outcome: At the end of the semester the student would have learnt about the basics of systemic. immuno. immune complex reactions. Leukemias and Multiple myeloma. Inflammation and Repair. Male and female genital system. W. Coagulation disorders. pigments. current topics. body fluids and stool. Haemoglobin estimation. cell injury and cellular adaptations. intracellular accumulations. obstructive and restrictive disorders. Diseases of the ear and eye. neoplasia and their effects causing morbidity and mortality.. 2001 Reference: 1. Introduction to Medical immunology 4th edition. Molecular events in cell growth and differentiation. Autoimmunity and Autoimmune diseases.S. Cellular and molecular immunology 2nd edition Abbas et al. Disorders of Kidney and Urinary tract: Congenital disorders. Mechanisms and general effects of cell injury. neoplasia. Tissue and cellular adaptations. Blood banking system and transfusion reactions. degeneration. inflammation. Apoptosis and Gangrene.saunders. Examination of urine. Mosby publishers 2009. immunological influences. environmental and immunological factors. Breast : Inflammation.. infections. Acquired Immunodeficiency Syndrome & Amyloidosis. inflammation. Classification and laboratory investigation. Preparation of smears and special staining methods. agranulocytosis. Blood group. Necrosis. 2. Unit II: Systemic pathology Cardiovascular system : Disorders of development. Gastrointestinal system. Primary Immunodeficiencies.and Co. Unit I : General Pathology Development of Pathology – Important milestones. Cytotoxic reaction. Department of Biotechnology   34  . Unit III : Clinical and chemical pathology RBC disorders – Anaemias. The structure of the cell.

Outcome: The students are expected to know a basic knowledge of neurology and the disorders of public interest related to it. David M Allen. Robbin’s Clinical Pathology. Focusing on risks to health is a key to preventing any disease onset. Health promotion and disease prevention are closely related. Oxford University Press. Screening techniques. 2000. James Carton. Peripheral nervous system – Autonomous nervous system – Sympathetic and parasympathetic. Neurological disorders are a significant and increasing public health problem. General Books Publishers. Laposata. Morphology of the brain and spinal cord. Preparations. 2. References: 1.Unit V: Histopathology Receiving of specimens. Unit I: Introduction to Nervous System Central nervous system – components. Quantitative Clinical Pathology.Mode of action. Pramila Ramani. Clinical Pathology. Text Books 1. 2009. Elsevier Science Health Science Div. Blackwell Publishers. Grossing Techniques. Fixatives . 2008. 10MB317 IMMUNOLOGY LAB Credits: 0:0:2 12 Experiments will be notified by the HOD from Time to Time 10MB318 MEDICAL LAB TECHNOLOGY LAB Credits: 0:0:2 12 Experiments will be notified by the HOD from Time to Time 10MB319 NEUROSCIENCE Credits: 3:0:0 Objective: Neurological services and resources are disproportionately scarce due to lack of knowledge and awareness. P. Indications. 2009 2. Clinical Pathology of the Blood. Staining principles. James Ewing. Hamilton. Processing of tissues for routine paraffin sections and other methods of embedding. Richard Daly. preparation of reagents and techniques. Decalcification of calcified tissue before sectioning. Department of Biotechnology   35  .

Neuroinfection. Neurological disorders associated with malnutrition. Esterase – inhibitors – neuro toxins.C. Automatic tissue processor. Unit II: Neurotransmission Transmission of nerve impulse – resting and action potentials – Polarization. Hematology. Geoffrey. Sodium/ potassium pump – Role of calcium. Gerald Karp. Chemical transmission – neurotransmitters – types. Traumatic brain injuries. (2008): Fundamentals of Anatomy and Physiology (prentice Hall. Guyton. mictotomes (various Department of Biotechnology   36  ..Structure and function of the nervous tissue. depolarization and repolarisation . Pain associated with neurological disorders. A. in Blood Banking. Unit IV : Neuromuscular Coordination Neuromuscular transmission. Multiple Sclerosis. Unit 1 : Medical Lab management Laboratory management and planning. (2007): Biochemistry – 4th Edition Reference 1. Zubay.E. Regulation of body temperature. (2008): Cell and molecular Biology. CSF. J. Interaction between sense organs and neurons. Saunders Co) 2. Knowledge of maintenance and use of the following : Microscope. Enzymatic inactivation of neurotransmitters. Forces involved in ligand – receptor interaction. Text Books 1. (2005): Textbook of Medical Physiology (W. (John Welly and Sons) 2.B.e. and Hall. Unit III : Neurotransmitters Receptors – adrenergic α and β receptors and cholinergic receptors. Ach. head ache. reflex action and reflex arc. Frederic Martini. vacuum embedding bath. New Jersey) 10MB320 MEDICAL LAB TECHNOLOGY Credits: 3:0:0 Objective Medical Laboratory Technology is an allied health profession which is concerned with the diagnosis and prevention of disease through the use of clinical laboratory tests. Synaptic cleft and neuromuscular junction. Epilepsy. Stroke. Unit V : Neurological Disorders Dementia –Alzheimer’s disease. Immunology and Microbiology. Regulation of transmission. synthesis and secretion of neurotransmitters. Parkinson’s disease. Types of neurons.I. Outcome The students are expected to be thorough in five major areas of the laboratory i. Clinical Chemistry.

2009. Criteria used while selecting a blood donor. ovens. Mukherjee. Kolkata. Examination of body fluids—pleural. II & III – K. vacuum embedding. Text books 1. Haemostasis. New Delhi. pericardial. Urine—its formation. Transfusion reactions—recognition and investigations. refrigerators. Inheritance and nomenclature of ABO and Rh blood group systems. cryostat. Peripheral blood smears. Salient features and investigations for iron deficiency anemia megaloblastic anaemia and haemolytic anaemia. 2. cerebrospinal fluid and semen examination.2008. hot plates. Staining procedures in Histopathology and haematology. 6/e. Examination of sputum. autoclaves Unit II: Histopathology Tissue processing —details of paraffin embedding. References 1. Different methods of estimation of hemoglobin. complementary and homeopathic medicine in managing health. CBS Publications &Distributors. Tata McGrawHill Publishing company.L. Leukaemias. Outcome: Department of Biotechnology   37  . I. Frozen section — uses and techniques. refrigerated centrifuge. Unit III: Haemotology Blood —Collection of haematological specimens. Decalcification. Unit V: Blood bank management Blood bank management and planning the receiving and recording of blood samples. 10MB321 COMPLEMENTARY AND ALTERNATIVE MEDICINE Credits: 3:0:0 Objective: The objective of the present subject is to provide a clinical application of alternative medicine practices including alternative. normal and abnormal constituents.Section cutting and different types of microtomes. Unit IV: Body fluids & excretory products Examination of stool. indexing.R. Maity.types with working of each). Walking coolers. Haemorrhagic disorders. Medical Laboratory Technology – C. 2005. ascitic. Medical Laboratory Technology Vol. Blood Bank refrigerators. incubators. Varley’s Practical Clinical Biochemistry –Alan H Gowenlock. New Central Book Agency Pvt Ltd. New Delhi. other blood group systems.

References 1. dance Movement therapy. MD. mud therapy. reflexology. Siddha system. Unani system. gem essence therapy. Ayurvedic medicine. Psychotherapy. Core concept. Department of Biotechnology   38  . Herron R. attitudes and skills necessary to communicate effectively with practitioners and users of complementary and alternative medicine. UNIT : II Traditional Indian System of Medicine Advantages and limitations of Ayurveda System. philosophies. UNIT : V Exercise and therapy Alcoholism . Levin. Jeffrey S. Colour therapy. Gandhi: Nature cure. Counseling. Sep/Oct 2002. Acupuncture. Faith healing.The paper will help the candidate to study about the healing paradigms. 2. Diet – health food –Herbal therapy -flower therapy – Megavitamin therapy –Fasting as therapy. 1999. Hypnotherapy. Chopra A. National Academic Press. Pyramid power.86(1):75-89. UNIT : I Basics of complementary and alternative medicine Health : The traditional approach -Approach in various systems of medicines and healing Emerging new visions from systems and holistic approach. reichian body therapy. UNIT : III Alternative medicines and therapy I Acupressure. relaxation technique. Textbooks 1. Med Clin North Am. and current relevance. therapeutic principles. Lipophil-mediated reduction of toxicants in humans: an evaluation of an Ayurvedic detoxification procedure. Music therapy. electro therapy. M. Wayne B. addictions and deaddication techniques -Responsible Parenthood -do’s and Don’t do’s for a health life – Prayer and spiritual dimensions of Healing –Place of Yoga and meditation. Primal therapy. Art therapy. PhD. theoretical basis and techniques of various complementary and alternative medicines. cymatics. Jonas. Charismatic healing Bioenergetics. 8(5):40-52. The Western System of (Allopathic) Medicine and its advantages and limitations.K.therapeutic touch . Essentials of Complementary and Alternative Medicine. This paper also describe the knowledge. shiatsu. UNIT : IV Alternative medicines and therapy II Hydro therapy. Polarity therapy. geopathic therapy. behavioral Therapy. MPH. Physical and psychic exercises . Doiphode V. Alternative Therapies in Health & Medicine. Homeopathy system. sound therapy. 2005. Complementary and Alternative medicine in the United States. 2002 Jan. 3. Naturopathy and Folk & Traditional system. Magneto therapy. Massage Techniques. Fagan J. Stephen Barrett. 2003 2. floatation therapy.

molecular approaches to cancer. and immune deficiency disease. RNAi. RDA. Transgenic animals as models for human disease.and Mendelian genetics. Pathophysiology & Mechanisms of Human Disease Unit II: From Gene to Protein to Disease Moleculaar diagnosis. Proteomics and Functional Genomics.Genetically engineered stem cells and experimental therapies. types of mutations and maintenance of genome integrity. Cellular and Molecular Biology of Disease. positional cloning. CGH. Stem cell based therapies: stem cells and repair of heart and nervous system. Experimental identification of oncogenes and tumor suppressor genes. brain cell transplantation and stem cells in aging Unit V: Personalized Medicine and the Right to Privacy(11) Method & Logic of Translational Biology. To Understand molecular medicine from a historical perspective as a simple extension of the mandate of science to improve the lot of humans and other life on earth. Genetic basis of cancer. and proteins. SKY. RNA. genetics of human psychiatric disorders. RNA. and virus studies Cytogenetics and cancer. mutation. Proteins and Cells.10MB322 MOLECULAR MEDICINE Credit:3:0:0 Objective To Recognize the relationship between technological advances and the effects arising as technologies become widely used. Microarrays. inborn errors of metabolism. Some commonly used techniques: the 2-hybrid system. G-banding. Ethics. Conduct & Practical Aspects of Clinical Research. Overview of the basic techniques used to study DNA. Text Books: Department of Biotechnology   39  . animal. LOH. Unit I : Historical Perspectives – Technology & Society The Molecular World: DNA. Introductory Biostatistics for Translational Research. autoimmune disease. regeneration strategies. and FISH. Unit III: Oncogenes The basic biology of cancer. cardiovascular disease.variation. Highthroughput sequencing. human. chromosomal rearrangements. Skin replacement. and genomics-based methods Unit IV: Genetic Disease – Genomic Considerations: General principles of inheritance. Outcome To learn the possible ways in which modern biochemistry and biotechnology could help in molecular diagnosis and treatment. Inherited predisposition to cancer Mutation and cancer. The structure and expression of prokaryotic and eukaryotic genes.

2002 Molecular Biology of the Cell. Seventh edition.1. 2. Jean Brachet and Alfred E Mirsky. 10MB323 CELL CULTURE TECHNIQUES Credits: 3:0:0 Objective: The aim of the course is to provide basic knowledge on cell and tissue culture. gene therapy for diseases. Peter. 2008. Keith. Lewis. Murphy. Roberts. Harvey Lodish et al. NY. Janeway’s Immunobiology the immune system in health and disease. immobilized cultures. Unit II: Human diseases and their diagnosis Bacterial and viral diseases in Humans. monoclonal antibodies and their use in diagnosis.equipments used in micromanipulation. in vitro fertilization and embryo transfer. strategies for the production of transgenic animals Department of Biotechnology   40  . Unit V : Transgenic Animals Concepts of transgenic animal technology.. Martin. Unit III: Therapy of Human Diseases Recombinant cytokines and their use in the treatment of infections. cell cultures as a source of valuable products. New York. somatic cell fusion. 2000. chemically defined and serum free media. Walter. enrichment of x and y bearing sperms from semen samples. various types of cultures. Johnson. Physiology and Morphology. organ cultures. vaccines and their applications in infections. Alexander. Unit IV: Micromanipulation of Embryos Micromanipulation technology . their maintenance and preservation. Outcome: At the end of the semester the student would have learnt about the basics and various methods of cell cultures. 3. Garland Science. Molecular Cell Biology – 4th ed. Alberts. molecular diagnostic techniques like PCR. W H Freeman. suspension cultures. Unit I: Basics of Cell culture Introduction to basic tissue culture techniques. animal cell cultures. Garland Science Publisher Reference: 1. Academic Press. Raff. continuous flow cultures. monoclonal antibodies in therapy. northern and southern blotting. Bruce. Traver & Walport. Julian. in-situ hybridization. 2003 The Cell Biochemistry. RFLP. artificial insemination and germcell manipulations.

(2002). stem cell cultures in the production of transgenic animals. Unit IV: Pharmacognosy 41  Department of Biotechnology   . 2.New Delhi. Biotechnology. ADME process. Gangal Sudha (2007). Respiratory System. Basic Biotechnology.(1995). Biotechnology. Ian (2006). Gastrointestinal System and Nutrition. Satyanarayana U. Integumentary system and Eyes/Ears. drug action. Culture of Animal Cells:A Manual of Basic Techniques. Inc. Ignacimuthu S. Ltd. Animal handling and animal care. (2007). Unit I : Basics of Pharmacology General concepts of Pharmacology. Methods of anaesthetising animals and methods of euthanasia. Antineoplastic agents. Arora M. Rastogi Pub. Biotechnology and Genomics. Freshney R.mechanism. factors affecting drug action. New York. 4.Himalaya Publishing House. 3. Text books 1. Chemotherapy of microbial diseases.Agrobios India.Meerut.Agricultural Biotechnology.Tata McGraw Hill Publishing Co.Ltd. Immune system. ex vivo). Dose-effect relationship Unit II : Systemic Pharmacology Drugs Affecting the Central Nervous System. Universities Press India Pvt. Chemotherapy of parasite infections. Drugs acting on blood and blood forming organs Unit III : Experimental Pharmacology Experimental methodologies involved in the discovery of drugs (in vivo. 2. Books and Allied Pvt. Gupta P.and their importance in biotechnology.P. Jodhpur. John Wiley and Sons. Outcome At the end of the semester the students would have learnt about basics of drug action in various system and natural sources as medicine.(2003). Principles and Practice of Animal Tissue Culture. Endocrine System. Drugs affecting uterine motility.. References 1.S. Kolkata 10MB324 PHARMACOLOGY AND PHARMACOGNOSY Credit 3:0:0 Objective Helps the student to understand and apply this knowledge in research to pharmacology and basics of pharmacognosy. Immunomodulators. 5. Cardiovascular and Renal Systems.. Restraining and blood collecting methods. Mumbai.K (2004). Purohit S.Ltd. in vitro.

Lippincott's illustrated reviews: Pharmacology by Richard a Harvey.E.Robbers. 2006. Luigi X Cubeddu (Editor) › Visit Amazon's Luigi X Cubeddu Page Find all the books. See search results for this author Are you an author? Learn about Author Central . Luigi X Cubeddu . B001IGSJLC 10MB325 MOLECULAR BIOTECHNOLOGY Credits: 4:0:0 Course Objective: A wide aspect of recombinant DNA technology. their merits and Demerit -a. Delhi. 2002. Pharmacognosy by G. Methods used to improve the Quality and d. R. General cultivation of medicinal plants. methods of isolation. General aspects.General aspects of sources of natural medicinal products. 4th edition. Wallis. 1988. c. ELBS. Pamela C Champe B000APHLSY Pamela C Champe (Editor) › Visit Amazon's Pamela C Champe Page Find all the books. Pharmacognosy by Varro E. Reference 1. 2008 2.E. Evans. Katzung. Text Books: 1. Text Book of Pharmacognosy by T.Brady. Role of natural pesticides – Preparation and uses. b. Factors involved. Richard Finkel. and more. Trease. CBS Pub. vaccine production with respect to quality analysis and quality control. 3. Course Outcome: The candidates can have an industrially applied knowledge on genetic engineering.1985. Unit I: Basic Molecular Biology Department of Biotechnology   42  . read about the author. Lynn. W. Gene expression – Analysis and vaccine production. Marine Pharmacognosy and its applications.C. Unit V: Isolation and Characterization of Phytochemicals General methods and Principles of extraction methods. 2. and more. types of extraction and their merits and demerits.Tyler. James E. purification and identification of phytoconstituents. read about the author. See search results for this author Are you an author? Learn about Author Central michelle a clarke. Basic & clinical pharmacology By Bertram G. Selection and purification of solvents for extraction. Pest control.

Transgenic animal (mice). Unit II: Recombinant DNA technology Introduction to rDNA technology. Panima. Gene therapy.W.PCR and realtime PCR.protein engineering by DNA shuffling to increase enzyme activity and protein stability. Principles of gene manipulation.Translation and post translation modification. 1998 References 1.yeast vectors.A. O.Knock out animal. Primrose. Brown. inhibition of protein synthesis – Regulation of Gene expression-lac operon. Unit I: Endocrine System General concept on Exocrine secretions. Department of Biotechnology   43  . Unit IV: Immunological diagnostic tools.D. 2.restriction endonucleases. Unit V: Applications of recombinant DNA technology.eukaryotic expression vectors. T. Ed. General concept on Endocrine Secretions – Structure and function of kidney – Basics of Animal Tissue culture. Blackwell Scientific publications. 2000 2. 2001 10MB326 MOLECULAR PHYSIOLOGY Credit: 4:0:0 Course Objective: Basic physiology of the human system is brought into limelight and their functioning is studied for application of nanotechnology. Genetics – a molecular approach.R.cloning. Affiliated East West Press Private Ltd. 2. 3 2003 2. Ed. stem cell therapy.Transcription. Unit III: Directed mutagenesis and protein Engineering Random and site directed mutagenesis(Kunkels method). Regulation of transcription .insitu vectors.FISH. A test book on Biotechnology. an introduction to genetic engineering. Recombinant antibodies and vaccines. Kumar H. Molecular and serological diagnostic tools-Monoclonal antibodies.vectors. Text books: 1.DNA replication . post-transcriptional modifications.mammalian and plant vectors. Molecular Biology and Biotechnology. New Delhi. Ed. Walker and Raplery. Liver and Pancreatic secretions. Course Outcome: This paper will be a bench marking for students proposing to do their higher studies in nanotechnology and biomedical implants design based on their physiological functions.

hypo and hypertension. conduction of nerve impulse.blood brain barrier. resting and action potential. Synthesis of collagen and their matrix components – elastin and other fibres. Nervous system: General organization – function units. Integrated control of mineral metabolism. electrophysiology of heart. Calcitonin and PTH. Interrelationship of calcium. L.D. G.Unit II: Muscular System Excitation and contraction of muscles – molecular organization of muscle. Unit – V: Metabolic System Mineral metabolism: organization of Bone-Matrix.M. Respiratory response to acid-base balance.Types of implants . Buffer systems of body fluids and their role. E. Talwar. Enkephalins and endorphins. K. epilepsy and psychoses. Unit I: Implant materials Steps in the fabrication of implants .S and Todd. Markers of bone metabolism. and abnormal electrolyte composition of blood – metabolic acidosis and alkalosis..: Text book of Biochemistry and Human Physiology. Course Outcome: The students will have a clear picture on the synthesis of biomateirals and their application in human physiological systems. Medical Physiology (WB Saunder’s Company) References 1. W. and Momdgill. metabolism and metabolic adaptation. Energy for contraction. Cardiac physiology – circulation. neuromuscular junction.Currently available degradable Department of Biotechnology   44  .P. compounds affecting synaptic transmission. Acid – base balance and status. synaptic transmission. Unit – IV: Nervous System Brain: chemical composition.R (1985) : Text book of Biochemistry (McMillan) 2. Respiratory mechanisms in acid-base balance. Unit – III: Circulatory System Blood gas analysis. Biochemical aspects of learning and memory. neurotransmitter and cAMP. Srivastava. Textbooks: 1. States of brain waves. Measurement of blood pressure. vitamin D. 10MB327 BIOMATERIALS Credit: 4:0:0 Course Objective: To give a broader perspective on biomaterials and their functions with human systems. Zubay (1998): Biochemistry – 4th Edition (WMC Brown Publishers) 2. Cells of bone and their biochemical characterization. Guyton (1999).Microstructures and properties of implant metals Hydrogen – properties and application . their organization and role in contraction. West. protein of contractile element.

5.targeting to specific organs or tumour types – tumour specific targeting – breast cancer.6.Types of bio-ceramics: Tissue attachment .liver cancer.Chemical and biochemical degradation of polymers .N. bioconjucation.targeting tumor vasculature for imaging delivery of specific anticancer agents such as Paclitaxel. Fujita.polymers . Association of implants with human and animal tumors . New York.M. antibody based and metabolism based targeting – Nanoparticles surface modifications. Kluwer academic publishers.Liposomes for pharmaceutical and cometics applications Liposomal drug carriers in cancer therapy– Lipid based drug delivery systems for peptide and protein drug delivery. “Molecular Modelling Principles and Application”. soft and rigid contact lenses .Mechanism of bioerotion and chemical degradation.targeting through angiogenesis. future directions Dental implant systems .Pathologic calciferation of biomaterial .Native Collagen and Elastin Chemical modifications of collagen Unit II: Biomaterial implants and biomaterial degradation.Contact lenses: Principles. Pomogailo. Ed.Leach. Plenum press. 2003 2. Pacemakers. Mitra Dutta.Implants of Cornea and Glaucoma .Nanoscaffold systems –Biocompatibility. Vena-cava filters and Total artificial heart .Water penetration controlled systems . Biomaterials.Virus based nanoparticles Unit V: Drug Carriers Nanoparticle and targeted systems for cancer diagnosis and therapy Synthesis.Targeted delivery through enhanced permeability and retention – folate receptors.Assessing calciferation of biomaterial . Narosa publishers. 2009 2. Micromachines as tools for nanotechnology. H.Haile. 2004 3. Michael Stroscio.Chemically controlled devices for drug delivery systems. 5. Springer. Unit IV: Principles of drug Delivery systems: Modes of Drug Delivery. Department of Biotechnology   45  .Ed.Blood substitutes. 2002 References 1. Nanocomposites. SV Bhat.nanoscale containers.Liposomal anticancer and antifungal agents. John Wiley and Sons. Text books: 1. Springer. Delivery of Drugs.Controlled drug delivery. multi-fuctional gold nanoparticles for drug delivery. 2.Prevention of calciferation. 1997. J. Kestelman. pegylation.In vitro and in vivo assessment of tissue compatibility . Longman. JB Park. Biomaterials Science and engineering.fluorouracil.ADME Hypothesis . 2005 4.Implant associated infection .Vascular grafts.D.Biocathetars. A. Biological nanostructures and applications of nanostructures in biology.Oxidative biodegradation . A.Pathobiology of foreign body tumorigenesis . “Molecular Dynamics Simulation Elementary Methods”. Hear valves . doxorubin.R. V. Unit III: Application of biomaterials in medicine Cardiovascular applications: Cardiopulmonary Bypass. 1996.

6. Satya Prakash Gupta, “QSAR and Molecular Modeling”, Springer - Anamaya Publishers, 2008.

10MB328 BIOLOGICAL AND BIOMEDICAL NANOTECHNOLOGY Credit: 4:0:0 Course Objective The principles and synthesis of nano scaffold preparations are offered in this paper. Course Outcome A detailed account of Drug development and binding mechanisms would be appreciated and furthur research cab. Unit I: Microspheres for drug delivery Introduction, Background - Fabrication of polymer micro and nanoparticles, Fabricating uniform microspheres - Percision particle fabrication technology - Fabricating uniform coreshell microparticles - Electrodynamic spraying - Controlled in vitro release from precision microspheres and mixtures of uniform microspheres - In vitro release with double-wall microspheres - Release from monodispense microspheres - Current status of using microspheres: a few case study Unit II: Inorganic binding polypeptides in bio-nanotechnology Selection of inorganic-binding polypeptide - Binding affinity: Molecular adsorption of GEPI - Physical specificity and molecular modeling - Potential applications of molecular biomaterials - Bio-nanofabrication: Silica synthesis using inorganic binding polypeptide Future prospects and applications in nanotechnology - Polymer design for non-viral gene delivery - Synthetic and Natural polymer genes carriers: examples - Biomaterials approach to gene delivery Unit III: Dynamic nanodevices based on protein molecular motors Protein molecular motors: Biophysical aspects - Nanodevices based on protein molecular motors, Motility assays and single molecule techniques - Interaction of motor protein with the device environment - Design, fabrication of protein molecular motor based nanodevices – Lateral confinement of movement - protein molecular motors. Unit IV: Nanoscale polymer fabrication for biomedical applications Biomedical applications of polymer nanostructures - Mold making and prototyping Assembly and bonding - Future directions in polymer fabrication - 3D microfabrication – definition, 3D-microparts for trans dermal drug delivery system - Emerging methods for 3D micro and nanofabrication, two photon assisted micro-fabrication – Nano imprint and soft lithography - Nanogaps for molecular conductivity measurements Unit V: Nanodevices in biomedical applications
Department of Biotechnology   46 

Integrating biomolecules into devices - Low throughput construction methods and “one-off” nanostructure - Biomolecules in therapeutic nanodevices, self assembly and orthogonal conjugation - Delimiting nano-therapeutic action in three dimensional space - Spatially and temporally delimiting nano-therapeutic action - Sensing approaches - Imaging using nanotherapeutic contrast agents - Hybrid nanotherapeutic devices in oncology – Nanotherapeutics for cardiovascular applications - Hybrid nano-therapeutics and specific host immune responses. Text books: 1. Biological nanostructures and applications of nanostructures in biology, Michael Stroscio, Mitra Dutta, Kluwer academic publishers, 2004 2. Nanomaterials for nanoscience and nanotechnology, Zhong Lin Wang, Wiley-VCH, 2000 References: 1. Biomolecules meet nanotechnology, Review article, Angew. Chem. Int. Ed., 2001, 40, 4128 -4158 2. Nanobiotechnology, C.M. Niemeyer, C.A. Mirkin, Wiley-VCH, 2004 3. The handbook of nanotechnology, J.C. Miller, R.M. Serrato, J.M.R. Cardenas, G.A. Kundahl, John Wiley & sons, 2004

10MB329 BIOTECHNOLOGY LAB - II Credit: 0:0: 2 12 Experiments will be notified by the HOD from Time to Time

10MB330 BIOTECHNOLOGY LAB - III Credit: 0:0: 2 12 Experiments will be notified by the HOD from Time to Time

Department of Biotechnology  

47 

Karunya University

Department of Biotechnology

SCHOOL OF BIOTECHNOLOGY AND HEALTH SCIENCES

Academic Information  

Karunya University

Department of Biotechnology

ADDITIONAL SUBJECTS

Sub. Code 11BT201 11BT301 11BT302 11BT303 11BT304 Biostatistics

Name of the Subject

Credits 4:0:0 4:0:0 3:0:0 3:0:0 3:0:0

Process Equipment Design and Drawing Enzyme Technology and Industrial Applications Advanced Environmental Biotechnology Advanced Animal Biotechnology

11BT201 BIOSTATISTICS Credits: 4:0:0 Course Objective To provide knowledge and skills sufficient to allow students to understand the role of statistics in biology research. To develop skill in the basic methods of data gathering and analysis. Course Outcome To provide sufficient background to be able to interpret statistical results in research papers. To develop sufficient knowledge of probability and probability distributions to support further studies in biostatistics and research. UNIT I Organization of Statistical investigation- object and scope, Classification and tabulation of dataIndividual, discrete and continuous series, Diagrammatic and graphical presentation of data using line, simple and multiple bar diagram, Pie diagram. Histogram and frequency polygon UNIT II Methods of sampling- Random and non-random sampling, Sampling distribution and test of significance: Testing of hypothesis and tests of significance, Standard error, Student's 't’ test, Chi - square test & goodness of fit- Simple problems. UNIT III Measures of central tendency: arithmetic mean, median, mode for individual, discrete and continuous data. Measures of Dispersion: standard deviation and coefficient of variance for individual, discrete and continuous data. Correlation analysis- Scatter diagram, Karl Peason's coefficient of correlation and Spearman's rank method, simple problems UNIT IV Regression analysis: Definitions, Graphical method and algebraic methods, Regression equations- Simple problems. Probability: definition, concepts, theorems (proof of the theorems not necessary) and calculations of probability- Simple problems.
Academic Information   1 

Karunya University

Department of Biotechnology

UNIT V Analysis of variance, Techniques of analysis of variance: one way and two-way classification of ANOVA. Text Book: 1. Biostatistics, Ramakrishnan, R. 2010, Saras Publications. Reference Books: 1. Statistical methods for Biologists, S. Palanichamy and M. Manoharan, 2009, Paramount Publications. 2. Statistical Methods, S. P. Gupta, 2008, S.Chand & Sons. 11BT301 PROCESS EQUIPMENT DESIGN AND DRAWING Credits 4:0:0 Course Objective To design safe and dependable processing facilities in a cost effective manner. This course focus on plant layout and design of piping systems. Course Outcome At the end of the course the student will be familiar in design and engineering principles involved in process plant layout and piping design. UNIT I: ENGINEERING PROPERTIES Selection of materials, Engineering properties of various materials at different temperatures. Factor of safety - Working stresses. Application and use of various codes and standards in design. Piping and Instrumentation Diagrams. UNIT II: STORAGE TANKS Design of tall vertical and horizontal vessel storage tanks. Foundations and supports for equipments and tanks. Internal and external pressure vessel calculations. UNIT III: FERMENTOR DESIGN Introduction to fermentor, types of fermentor, fermentor accessories and various parameters for design, Design of airlift fermentor. UNIT IV: SEPARATION EQUIPMENT Types of centrifuge, Design of top suspended bottom discharge centrifuge; types of filtration, design of plate and frame filter press; design of swenson walker crystallizer. UNIT V: HEAT & MASS TRANSFER EQUIPMENTS

Academic Information  

Karunya University

Department of Biotechnology

Introduction to heat transfer and mass transfer, Design of 1-1shell and tube heat exchanger; design of single and multiple evaporator; design of counter current extraction; types of dryer and design of rotary dryer.
TEXT BOOKS:

1. Indian Standard Codes: (a) IS : 2825 - 1969: Code for Unfired Pressure Vessels. (b) IS : 4049 - 1979: Specifications for formed ends for Tanks and Pressure vessels. (c) IS : 4179 - 1967: Sizes of Process Vessels & their Leading Dimensions. (d)IS: 4864 to 4870 - 1968: Specifications for Shell Flanges for Vessels and Equipment. (e) IS : 4503 - 1967: Specifications for Shell & Tube Heat Exchangers. (f) IS : 803 - 1962: Code of practice for Design, Fabrication and Erection of Mild Steel Cylindrical Welded Oil Storage Tanks. (Published by Bureau of Indian Standards, New Delhi). 2. Joshi, M.V. Process Equipment Design, IIIrd Edn 2004 , MacMillan, India,.
REFERENCE BOOKS:

1. Perry, R.H. & Green, D.W.: Perry’s Chemical Engineers' Handbook,7th Edn 2000, McGraw Hill. 2. Coulson, J.M., Richardson, J.F. & Sinnott, R.K. Chemical Engineering, Vol. VI 1999, MaxwellMacmillan, New York. 3. Brownell, L.E, & Young, E.H. Process Equipment Design 2004, Wiley Eastern, New Delhi

11BT302 ENZYME TECHNOLOGY AND INDUSTRIAL APPLICATIONS Credit: 3:0:0 Course Objective To understand the mechanism of Biocatalyst and the kinetics behind its extraction and purification procedures, immobilization techniques etc. Course Outcome The students will be able to understand the concept of immobilization extraction and purification and the inhibition kinetics of the enzymatic reactions UNIT-I CLASSIFICATION AND MECHANISM OF ENZYME ACTION Classification of enzymes, quantification of enzyme activity and specific activity. Effect of pH and temperature on enzyme activity. UNIT-II KINETICS OF ENZYME ACTIONS Estimation of Michaelis Menten parameters, Enzyme Inhibition – Substarte, Product and Toxic compound inhibition, types and derivation. UNIT-III APPLICATION OF ENZYMES Extraction of commercially important enzymes from natural sources; Commercial applications of enzymes in food, pharmaceutical and other industries; enzymes for diagnostic applications.

Academic Information  

which can be exploited to save environment from eventual deterioration.F.Kyoto protocol. D. Bonner.Karunya University Department of Biotechnology UNIT-IV MASS TRANSFER EFFECTS IN IMMOBILISED ENZYME SYSTEMS Techniques of enzyme immobilisation Analysis of film and Pore diffusion Effects on kinetics of immobilised enzyme reactions. T. Bailey J. 2nd Ed 2010. the students would have learnt about the environmental related issues and the Problem solving ability UNIT I ENVIRONMENTAL POLLUTION AND SOCIAL ISSUES Definition and Scope of Environmental Biotechnology. Conversion of Solid Waste to Methane. Biochemical Engineering Fundamentals. Clinical chemistry. ENERGY AND SOLID WASTE MANAGEMENT Biomass waste as renewable source of energy. Palmer. Social Issues. acid rain and nuclear holocaust. Methods of energy production. P. Water recycling methods. Management of Academic Information   4  .E. Design of enzyme electrodes and case studies on their application as biosensors in industry. UNIT II INDUSTRIAL WASTE WATER MANAGEMENT Purification of waste water. Harwood Publishing Ltd. Enzymes: Biochemistry. Control measures of environmental pollution. Course Outcome At the end of the course.Green House Gases. & Ollis. Management of radioactive pollutants. Aerobic and anaerobic treatments. 2nd Edn 2007. McGraw Hill. healthcare and environment. Biotechnology. Biofuels and Microbial fuel cells. Global Warming-Climate change. Biogas production.L. 11BT303 ADVANCED ENVIRONMENTAL BIOTECHNOLOGY Credits: 3:0:0 Course Objective To develop skills of students in various biological phenomena. Laboratory methods for the detection of coli form organisms in water. Text Books: 1. UNIT III BIOMASS. Reference Book 1. Formulation of dimensionless groups and calculation of Effectiveness Factors UNIT-V ENZYME BIOSENSORS Applications of enzymes in analysis.

animal and microbial DNA Sequences. fluidized beds. Primary and Secondary culture. Text Books: 1. Bailey J. Mc Graw Hill. Microencapsulation. Environmental Biotechnology 2005. 2006. IPR and Patenting.F. UNIT IV BIODIVERSITY AND CONSERVATION Definition. MacMillan Publications. Production of Vaccines and pharmaceutically important proteins in animal cells. Biochemical Engineering Fundamentals 2010. UNIT 1: CELL CULTURE CONCEPTS Aseptic techniques. Suspension culture.. Organ culture. Pesticides. UNIT III APPLICATIONS OF ANIMAL BIOTECHNOLOGY Xenotransplantation. media and reagents. maintanance. embryo culture. General Microbiology.Y. Biodiversity at Global Levels. Indu shekhar Thakur. Types. genetically Engineered Microbes for Bioremediation Biodegradation of Hydrocarbons.Land filling.R. Oxford university press.. D. Painter R. Patenting of plants. xenotransplantation. Dubey. Human Gene therapy (including the principle and strategies followed in si RNA and ribozyme methods) –vectors used. Ecosystem. C. UNIT II: LARGE SCALE CELL CULTURE OPERATIONS Scale up in monolayers. contamination. Course Outcome At the end of the course the students would have learnt about strategies and methodologies. limitations and applications.E. Types. Cell culture reactors.. Stanier R. I. USA 11BT304 ADVANCED ANIMAL BIOTECHNOLOGY Credits 3:0:0 Course Objective To impart knowledge on techniques involved in animal biotechnology. Herbicides. Species. International Pvt. Composting and Vermi Composting. Chand & company Ltd 2. K. perfusion. types. Reference Books: 1. Mixing and aeration. Characterization.Basic concepts and Applications 2006. preservation. Expression of recombinant proteins in Academic Information   5  . Wheels M. Plant Breeders Right and Farmers Right. R. Environmental Biotechnology.Ex situ and In situ Bioremediation. UNIT V BIOREMEDIATION AND BIODEGRADATION Definition. Alam Scragg. Ltd. Insecticides and Xenobiotics. cloning.Ecofriendly agriculture. Textbook of Biotechnology. S. strategies involved in stem cell culture and their therapeutic applications.L. Ingraham J. & Ollis. 2005.L. transgenic animals etc. Genetic. 2nd Ed.Karunya University Department of Biotechnology Sludge and Solid waste treatment. 3. lagooning. 2.

Manjula Shenoy. Spier RE and Griffiths JB. Reference Books: 1. mice (biopharming in animals) –as bioreactors UNIT V: IPR. human gene therapy and xenotransplantation Text Books: 1.applications UNIT IV: ANIMALS AS BIOREACTORS Transgenic animals – Production and applications of commercially important proteins. 2. Freshney. 2007. Portner R. 2008. fish. Vol VI. Gene knock-outs in mice. Academic Information   6  . 2009. Academic Press. cattle. Wiley-Liss. moral and ethical considerations in Animal Biotechnology. 2006. BIOSAFTEY AND ETHICAL ISSUES IN ANIMAL BIOTECHNOLOGY Patents and Patentable Inventions. Culture of Animal cells. over production and downstream processing of the expressed proteins. Animal Cell Biotechnology. Humuna Press. 2. Animal Biotechnology. ELSI (Ethical. 6th Ed. 4th Ed.Karunya University Department of Biotechnology insect (baculovirus) and animal cell – Expression vector. Biosaftey and levels. Social. Animal Cell Biotechnology. silk worm. legal and social impacts) related with stem cell technology. Laxmi Publishers.

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