Determining the protein concentration (Lowry

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Solutions Solution A: 2% Na2CO3 in 0.1N NaOH Solution B: 1% CuSO4.5H20 Solution C: 2% sodium tartrate Solution D: 1ml solution B + 1ml solution C Solution E: 49ml solution A + 1ml solution D Solution F: Folin–Ciocalteu reagent (1:1 diluted with dH20) For 1.0ml Sample solution are used 5ml fresh solution E. After 10min incubation in room temperature are added 0.5ml from Solution F. After 45min the absorption is measured at 750nm.

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