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Abstract: 6105

A25 - Mechanisms of immune dysfunction 14 A25


IMMUNOGENETIC FACTORS RELATED TO CERVICAL INJURIES IN A GROUP OF MEXICAN HIV-
POSITIVE WOMEN

M.d.R. Rocha Rodriguez


Autonomous University of San Luis Potosí, Faculty of Nursing., Biologic Molecular, san luis potosi, Mexico

Background: The incidence of cervical cancer constitutes one of the main health problems in developing countries, including
Mexico, where this disease represents the most common gynecological cancer. Multiple types of Human Papiloma Virus (HPV)
have been detected in patients with the Human Immunodeficiency Virus (HIV), increasing the risk of developing cervical injuries
in these women.
Methods: We selected 40 patients with HIV-1 who belonged to the COGUSIDA (State Aids Council in Guanajuato) and 26
women without HIV-1, selected from the State Aids Council in San Luis Potosí, Mexico. The data collection instrument
contained 42 items that explored personal data and information related to the acquisition, installation and progression of
cervical injuries. HPV typing was performed through the PCR-RFLP method, using the restriction enzymes Ava II, Rsa I, Ava I,
Bgl II, Acc I. For the TNF polymorphisms in regions 238 and 308, we used oligonucleotide probes with specific sequences,
hybridized with DNA amplified through the polymerase chain reaction, using the PCR-SSP technique.
Results: The 66 patients selected for the study were between 16 and 60 years old. Forty (66.6%) women were infected with
HIV-1 and 26 (43.3%) were not. Eighty percent of HIV-1 were asymptomatic and 20.0% had aids. All patients were infected by
HPV and all women reported that they already been infected with some kind of Sexually Transmitted Disease. For TNF-238,
the allele TNF-238*G was significantly increased among HIV-1 patients in comparison with patients without HIV-1 (p=0.00163,
OR= 0.7335).
Conclusions: This study suggests that the immunogenetic factors related to the magnitude of the TNF expression and
infection by HPV are associated in the development of cervical injuries in a group of Mexican women with or without HIV-1.

Country(ies) of Research: Mexico


Presenter: Ms. IMMUNOGENETIC FACTORS RELATED TO CERVICAL INJURIES Ma. del Rocio Rocha Rodriguez,
Autonomous University of San Luis Potosí, Faculty of Nursing., san luis potosi [Mexico]
Prizes/Awards: Women's Prize, ANRS/IAS prize, IAS Young Investigator Award