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Lab 1 Worksheet: Dependent and Independent Variables

Student instructions: Follow the step-by-step instructions for this exercise found on the worksheet below and in the virtual lab and record your answers in the spaces below. Submit this completed document by the assignment due date found in the Syllabus. Please make sure that your answers are typed in RED. Please type your Name and Student ID Number here: Keith Jones / Student ID: 4173049 Directions 1. Open the virtual lab titled “Dependent and Independent Variables.” 2. The screen will display the virtual lab simulation on the right side, and a “Questions” column on the left side. 3. Read all information in the Greenhouse Handbook. To open the handbook, click on “Greenhouse Handbook” on the shelf above the growth chamber in the lab simulation. 4. Read all background information and instructions in the “Questions” column. 5. If you come upon terms that are unfamiliar to you, please refer to your textbook for further explanation or search the word here: 6. Answer Lab Questions 1-5 below. 7. Follow the procedure below to complete the lab experiment. There are four seed varieties: two transgenic varieties—BT 123 and BT 456—and two non-transgenic varieties—Golden Crop and Super Harvest. You must gather data for all four varieties at levels of no infestation, low infestation, and high infestation. You may need to reset the simulation several times to see and select each of the four varieties. Record all of your data in Tables 1 and 2 below. Procedure

1. Click and drag a seed variety chambers.

to each of the two growth


You will see an animation of the calendar indicating the number of days that have passed. To calculate the Percent (%) Reduction in Yield for one seed variety use the following formula: 2 . Click and drag a level of ECB infestation the growth chambers.2. The selection will be applied to each plant in the growth chamber and the label will be updated with the information of the level of the ECB treatment. The yield of kernels from each plant is now on a weighing dish. 9. Drag a weighing dish to the balance to read the weight of the kernels. 6. a low level of infestation. Calculate the Average Yield for each seed variety at each level of infestation. the chamber doors open and the fully grown plants are evaluated. At the end of the time period. 10. Click the reset button to repeat the experiment by changing the variable of the ECB infestation level and/or seed variety. Record the weight in Table 1 below. Click on the reset button to choose from other varieties of corn. Click on the Grow button. To calculate the Average Yield for one seed variety at one level of infestation use the following formula: Average Yield = (Pot 1 yield + Pot 2 yield + Pot 3 yield)/3 *Round all average yields to the second (hundredth) decimal place. When all data are recorded in Table 1. 8. Proceed to weigh the kernel yield from each plant. or a high level of infestation for each seed type. You may choose to have no infestation. The seeds will be planted in all three pots and each growth chamber will automatically be labeled. to each set of plants in 4. 7. 5. You may choose to plant one or two different types of corn. Calculate the average kernel yield from each set of three plants that were grown under identical experimental conditions from the same type of seed. Click and drag each ear on each of the plants to a separate weighing dish. calculate the Percent (%) Reduction in Yield for each seed variety. 3.

How many days are required for a corn seed to become a mature plant with maximum weight kernels ready to be harvested? a. “BT Corn” refers to corn that: a. An insect pest that reduces corn yield d. 5. Answer Lab Questions 6-12 below. Edible corn byproducts c. yield with no infest. Is not affected by pesticides 4. yield with high infest. About 140 d. a corn plant d. A genetically engineered corn product. A bacterium that has a gene for producing Cry proteins. Record this data in Table 2 below. all of the above contribute genetic material to the production of BT corn Table 1: Average Yield (g) for each seed variety at no. About 65 c. ECB refers to which of the following? a. 11. Has been infected with bacteria c. About 23 b. Lab Questions 1. A stomach poison produced by bacteria. d. European corn borer e. and high infestation levels 3 . What is BT? a.)/avg. Has been infested with insect pests b. Is resistant to ECB d. – avg. b. About 180 3. A genetically engineered plant that is resistant to insect pests b. Bacillus thuringiensis c. yield with no infest. Both c and d 2. c. A bacterium carried by the European corn borer. low.Percent (%) Reduction in Yield = (avg. X 100 *Round all Percent (%) Reduction in Yield values to the second (hundredth) decimal place. European corn borer b. Creation of BT corn requires genetic material from all of the following except: a.

43 Avg.73 173.03 186 184.17 BT 456 None Low High Golden None Low High Super Harvest None Low High Table 2: % Reduction in yield for each seed variety at high levels of infestation (Transfer data on Average Yield (g) with No Infestation and High Infestation from Table 1 to Table 2) Seed Variety BT 123 BT 456 Golden Super Harvest Avg.77 184.9 135.9 184. Yield with High Infestation 160.67 157.97 160.3 161.5 130 Average Yield 163.6 138.03 164.2 139.3 163.8 179.3 155 129 Pot 3 Yield 164.42 21.9 157.20 128.4 164.17 % Reduction in Yield 1.2 168.45 15.6 163 183.2 172.8 162.57 4 .8 170.67 186 177 157.23 137.8 171.5 Pot 2 Yield 164.73 163.3 181.1 159.8 157.6 164.77 163.1 164 155.1 125.Seed Variety BT 123 Level of ECB Infestation None Low High Pot 1 Yield 160.77 128.6 177.77 137. Yield with No Infestation 163.6 157 182.6 159 189.1 190 178.43 157.18 25.

5g of the other? Explain your answer. He hopes this will slow the evolutionary development of BT resistant insects. Which transgenic seed variety was most resistant to the ECB at high infestation levels? BT 123 9. Which seed variety should he use to plant the 10% of his field that is not planted with BT 123 seeds? Explain your answer. No. If you compared one pot of Super Harvest and one pot of BT 123 with no infestation. Is there any significant difference in the average yield? No significant difference 11. the overall results were based on an average of three pots per seed. For each seed variety.6. Compare the yield of Super Harvest seeds and BT 123 seeds under conditions of no infestation. 12. Golden seed variety should be used because the average yield is higher than the Super Harvest variety 5 . A farmer decides to plant 90% of one field with BT 123 seeds and the remaining 10% of the field with a different seed variety. would you expect the yield of each pot to be within 0. why did you need to collect data from 3 pots for each infestation level to obtain reliable data? In order to get an average number a larger number of pots needed to be collected 7. Which seed variety has the highest yield under conditions of no infestation? BT 456 8. Which non-transgenic seed variety was most resistant to the ECB at high infestation levels? Super Harvest 10.

6 .