You are on page 1of 2

Anticoagulation and Preservation

Various anticoagulant-preservative solutions have been formulated for better red cell preservation, which are listed below, WHOLE BLOOD Acid Citrate Dextrose (ACD) Citrate phosphate Dextrose solution (CPD) Citrate phosphate dextrose adenine. (CPDA-1) Heparin EDTA (Ethylene diamine tetra acetic acid). RED CELLS Optimal additive solutions CPD - SAG (Saline, adenine, glucose) CPD - SAGMAN (Saline, adenine, glucose, mannitol) CPD - ADSOL (Adenine, saline, glucose, mannitol) RED CELLS - FROZEN STATE High glycerol solution Low glycerol solution Citrate Based Anticoagulants The importnat citrate based anticoagulant - preservative solutions used are ACD, CPD, CPDA (CPDA-1 and CPDA-2). A. Acid citrate dextrose (ACD): The most widely used ACD solution has the following formula : Trisodium citrate 22.Og Citric acid (monohydrate) 8.0 g Dextrose (monohydrate) 24.6 g Distilled water to make upto IL For each 100 ml of blood, 15 ml of the ACD solution (pH 5.0) is sufficient (e.g. for 450 ml, 67.5 ml of ACD solution is required). Preserves ATP level helps maintain red cells shape. prevents haemolysis maintains pH Shelf life of whole blood / red cells in ACD = 21 days (> 70% transfused cells viable after 24 hours)

B. Citrate phosphate dextrose (CPD) : CPD Solution  decreases acidosis  improves ATP synthesis Shelf-life of whole blood in CPD = 28 days

safe . Citrate Phosphate Dextrose Adenine (CPDA): Adenine .well tolerated .C.shelf-life = 35 days CPD-OPTOSOL =42 dias .helps maintain high ATP levels Blood collected in CDPA .