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Quiz Microbiology

Quiz Microbiology

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1.1) Which of the following is most likely caused by Legionella?

a) Bloody diarrhea

b) Fever lasting less than 24 hours

c) Atypical pneumonia

d) UTI

e) Meningitis

1.2) Which of the following is the most likely location to find Legionella pneumophila?

a) Water

b) Soil

c) Food

d) Plants

e) Desert

2.1) What kind of media is most commonly used to grow Legionella?

a) Chocolate (CHOC)

b) Blood agar plate (BAP)

c) Bile Esculin agar (BEA)

d) MacConkey agar (MAC)

e) Buffered charcoal yeast extract agar (BCYE)

2.2) What growth factors are required for Legionella?

a) Heme (X)

b) NAD (V)

c) Heme and NAD (X and V)

d) Cysteine and iron

e) Cysteine and heme (X)

2.3) Which of the following laboratory tests for Legionella uses indirect fluorescent

antibody (IFA)?

a) Culture

b) Urine antigen test

c) Nucleic acid amplification assay

d) Serology

e) Identification (microscopy)

Match the following clinical illness with the organism:

3.1) Periodontitis, endocarditis, bacteremia from dog or cat bites a) Streptobacillus

3.2) Rat-bite fever (irregular fever)

b) Capnocytophagia

3.3) Subacute endocarditis

c) Cardiobacterium

3.4) Trench fever (severe headache), cat-scratch disease, angiomatosis d) Bartonella

Microbiology

Quiz

Version: 03Jun2008

Page 49 of 66

40 – Anaerobic, Spore Forming, Gram-positive Rods

1) Which of the following is NOT true (in general) for Clostridium?

a) Gram-negative

b) Bacilli shaped

c) Spore-forming

d) Obligate anaerobes

e) Unable to reduce sulfate to sulfite

2.1) A teenage girl enters the emergency room suffering painful muscle spasms.

Throughout her examination, she sustains a facial sneer, a stiff arched back, and clamped

palms. Her father is anxious about the fact that she has also experienced difficulty eating,

probably due to a stiff jaw. The father affirms that her daughter is usually quite active and

boasts how, a week ago, she continued a soccer game even after falling on a nail in the

field. Which of the following is the most likely cause?

a) C. difficile

b) C. perfingens

c) C. septicum

d) C. botulinum

e) C. tetani

2.2) A woman straggles into the emergency room with a marked paralysis of her upper

body. She describes the paralysis as a weakness that began in her neck and spread to her

arms. She also complains of blurred double vision and requests water to soothe her dry

throat. Though she has no fever, she appears quite dizzy and her eyelids are drooping.

The day before, she returned from a camping trip where she insists she maintained good

hygiene, limiting her diet to canned food only. Which of the following is the most likely

cause?

a) C. difficile

b) C. perfingens

c) C. septicum

d) C. botulinum

e) C. tetani

2.3) An old woman comes to the doctor with a fever and loose bowels. Her diarrhea

occurs in tremendous volumes, she complains, though she doesn’t remember seeing

blood. She has an unremarkable recent past medical history, except for an infection a few

weeks earlier that was treated with clindamycin. Sigmoidoscopy of her colon reveals

yellow-white plaques which the doctor predicted after analyzing her stools for toxins.

Which of the following is the most likely cause?

a) C. difficile

b) C. perfingens

c) C. septicum

d) C. botulinum

e) C. tetani

2.4) A man enters the emergency room claiming to have been stabbed two days earlier.

Muscles in his arm hurt, and on palpation small air bubbles are felt below the skin. The

wound area exudes a blackish, ill-smelling fluid that generates a crackling sound when

touched. The patient has a fever, a low blood pressure, marked tachycardia, and urinated

Microbiology

Quiz

Version: 03Jun2008

Page 50 of 66

very little since his injury. The doctor decides to amputate the arm, as well as monitor the

patient for shock and renal failure Which of the following is the most likely cause?

a) C. difficile

b) C. perfingens

c) C. septicum

d) C. botulinum

e) C. tetani

2.5) Which of the following is a cause of nontraumatic myonecrosis and often exists in

patients with occult colon cancer, acute leukemia, or diabetes?

a) C. difficile

b) C. perfingens

c) C. septicum

d) C. botulinum

e) C. tetani

2.6) Which of the following is associated with Floppy Baby Syndrome?

a) C. difficile

b) C. perfingens

c) C. septicum

d) C. botulinum

e) C. tetani

2.7) Which of the following is associated with Pseudomembrane Colitis (PMC)?

a) C. difficile

b) C. perfingens

c) C. septicum

d) C. botulinum

e) C. tetani

3.1) Which of the following describes the effect of tetanus toxin?

a) Unregulated excitatory synaptic activity in the motor neurons

b) Blocks neurotransmission at peripheral cholinergic synapses

c) Induces depolymerization of actin with loss of cellular cytoskeleton

d) Produces chemotaxis; induces cytokine production with hypersecretion of

fluid; produces hemorrhagic necrosis

e) C and D

3.2) Which of the following describes the effect of botulinum toxin?

a) Unregulated excitatory synaptic activity in the motor neurons

b) Blocks neurotransmission at peripheral cholinergic synapses

c) Induces depolymerization of actin with loss of cellular cytoskeleton

d) Produces chemotaxis; induces cytokine production with hypersecretion of

fluid; produces hemorrhagic necrosis

e) C and D

3.3) Which of the following describes the effect of C. difficile toxin?

a) Unregulated excitatory synaptic activity in the motor neurons

b) Blocks neurotransmission at peripheral cholinergic synapses

c) Induces depolymerization of actin with loss of cellular cytoskeleton

d) Produces chemotaxis; induces cytokine production with hypersecretion of

fluid; produces hemorrhagic necrosis

Microbiology

Quiz

Version: 03Jun2008

Page 51 of 66

e) C and D

41 – Anaerobic, Non Spore Forming, Gram-positive Rods

1.1) What is the best description of the shape of Actinomyces?

a) Coccus

b) Bacillus

c) Spirillum

d) Diplococcus

e) Filamentous

1.2) Actinomyces grow ____ in culture and are facultatively anaerobic or strictly ____.

a) Quickly; Aerobic

b) Quickly; Anaerobic

c) Slowly; Aerobic

d) Slowly; Anaerobic

2) Comparing the two, Acinomyces ____ sulfur granules and Nocardia is ____.

a) Form; Acid-fast

b) Form; Not acid-fast

c) Does not form; Acid-fast

d) Does not form; Not acid-fast

3) Which of the following would lead to a clinical diagnosis for Actinomyces?

a) Gram-negative, beaded filaments, not acid fast, obligate anaerobe

b) Gram-positive, beaded filaments, not acid fast, obligate anaerobe

c) Gram-negative, rods, acid fast, obligate anaerobe

d) Gram-positive, rods, acid fast, obligate aerobe

e) Gram-negative, cocci, not acid fast, obligate anaerobe

4.1) Which of the following forms of actinomycosis (painful abscesses, growing larger as

the disease progresses) is the most common?

a) Cervicofacial

b) Thoracic

c) Abdominal

d) Pelvic

e) Central nervous system

4.2) Which of the following disease is often confused with actinomycosis?

a) Endocarditis

b) Herpes

c) Neoplasms

d) Osteomyelitis

e) Chagas disease

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