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Code: R7222303

R7

II B. Tech II Semester (R07) Supplementary Examinations, April/May 2012 INSTRUMENTAL METHODS OF ANALYSIS (Biotechnology) Time: 3 hours Max Marks: 80 Answer any FIVE questions All questions carry equal marks ***** 1 Write an account on the instruments used for analysis. Add a note on their sensitivity and detection limit. Write an account on the operation and uses of the following: (a) Phase contrast microscope. (b) Flow cytometry. Explain the general principal and operation of different types of centrifugations. Answer the following: (a) Beer Lamberts law and apparent deviation. (b) UV VIS spectrophotometer. Write an account on the principle, operation and applications of mass spectroscopy. Discuss the significance of pH, temperature, dissolved oxygen and sensors. Explain the principle and operation of gas and affinity chromatography. Describe the instrumentation and applications of high resolution NMR. *****

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