Word in Grey-camera view Words in black – voice over text Step 1 The view of the cell Step

2 Camera moves from the full cell view to the cell’s nucleus zooming in the nucleus pores Step 3 Camera moves from the nucleus pores view to chromosome and then zooms in part of it, so the DNA is clearly seen ‘DNA replication is a part of interphase, during which sister chromatids are created prior to cell division. ‘ Step 4 Helicase appears separating leading and lagging strands from each other and camera starts to follow moving helicase. ‘An enzyme, helicase, separates DNA strands into single strands known as the leading strand and lagging strand. ‘ Step 5 Proteins bind with the single strands of DNA. Helicase is still seen ‘To prevent them coiling, every strand binds with proteins.’ Step 6 Camera zooms out showing Polymerase encoding leading strand and DNA primase creating DNA primes. ‘The leading strand is replicated by DNA polymerase. DNA polymerase replicates the leading strand from its 5 prime end to its 3 prime end.’ ‘The lagging strand is replicated backwards which makes it more complicated to replicate.’ Step 7 Camera zooms in showing lagging strand ‘DNA primase creates RNA primers called okazaki fragments’ Step 8 Polymerase moves to lagging strand and start filling loops.

‘In between the okazaki fragments are loops, which DNA polymerase fills with DNA.

Step 9 Polymerase 1 appears and replaces DNA primes with actual DNA ‘Another polymerase now replaces the RNA primers with actual DNA and fills the gaps.’ Step 10 Ligase appears and binds okazaki fragments ‘An enzyme, ligase, then connects the extended okazaki fragments. ‘ Step 11 Camera zooms out showing all the DNA and all encoding process altogether Step 12 Camera moves to the end of the DNA, so we can see the ends of the DNA strands ‘Each time DNA replicates, one RNA primer is removed from the daughter strand’s 5 prime end. Each time DNA is duplicated it becomes one RNA primer shorter on the beginning of the leading strand and one RNA Primer short on the end of the lagging strand’ Step 13 The prime from the start of the leading strand and the prime from the lagging strand disappear. ‘Each DNA contains the protecting end known as telomeres. Telomeres do not store important genetic information.’ ‘Cellular aging occurs when telomeres are very short, making it impossible for the cell to divide and triggering senescence.’ Step 14 The view fades out