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Dr.

Niranjan Murthy H L
Asst. Prof., Dept. of Physiology
Sree Siddhartha Medical College & Hospital, Tumkur
STRUCTURE OF SKELETAL
MUSCLE

• Forms 40% of body weight


• Locomotion
• Striated or voluntary
• Multinucleated
Sarcolemma-
• Consists of an inner plasma membrane and
outer collagenous layer
• Invaginates at numerous points to form T-
tubules
• Carries action potential
Sarcoplasm-
• Consists of myofibrils
• Numerous mitochondriae
lying parallel to myofibrils
Sarcoplasmic reticulum-
• L-tubules
• Storage of calcium ions
• Skeletal muscle
• Fasciculus
• Muscle fibers
• Myofibrils
• Myofilaments
• Muscle fiber- 10 to 80μ in diameter each is
composed of 1000s of myofibrils

• Each myofibril is in turn made up of


myofilaments

• Myofilaments
(i) contractile- myosin II, actin
(ii) modulatory- tropomyosin, troponin
LIGHT MICROSCOPIC STRUCTURE
• Cross striations due to alternate dark and light bands
• Light band- Isotropic band- I band- thin filaments
• Dark band- Anisotropic band- A band- birefringent
• H zone- lighter zone in A band
• Z line- in the center of I band
• M line- in the center of A band
• Sarcomere- between two z lines- basic unit of muscle
ULTRASTRUCTURE
• Myofilaments- thick & thin filaments
• Myosin-10 to 14nm wide & 1.6μm long
1500 in each myofibril
Myosin filament made of around 200molecules of myosin
• Myosin molecule- composed of 6 polypeptide chains- 2 heavy
chains and 4 light chains
A tail, an arm and 2 globular heads
Arm and head form cross-bridge
Two hinges
Head has sites for ATP and actin binding
• Actin filament- 7nm wide and 1.0μm long
Extend on both sides of z-lines
F-actin forms a double helix
Made of 300 G-actin molecules (MW 42,000)
G-actin has active sites for interaction with
myosin heads
• Tropomyosin- 40nm length and MW of
70,000.
Wrapped around actin helix & covers
active sites in resting phase

• Troponin- made of 3 protein subunits


(Tn I, Tn C & Tn T)
OTHER PROTEINS

3) Actinin- binds actin to Z lines

2) Desmin- binds Z lines to the plasma


membrane

3) Titin- connects Z lines to M lines and provide


scaffolding for sarcomere. Provides elasticity

4) Dystrophin, dystroglycans and sarcoglycans