Experiment I: GIT Motility Test (ANTI-DIARRHEAL EFFECT) Charcoal Tracing Method

Acosta Advincula Afalla Agaton

Objective: To determine the effect of Atropine SO4 on the GI motility of the specimen (white rat).I. .

weigh the mice 3. Procedure (charcoal tracing method) 1. fast the mice for 12 hrs prior to test 2.II. administer appropriate amount of atropine SO4 based on the weight .

II. Procedure (charcoal tracing method) 4. 5. let it (atropine) take effect for 2030 mins. feed with atleast 1ml of the charcoal meal 6. wait for 20-30 mins to allow digestion .

Extract the SI from the pyloric end of the stomach to the distal ileum (ileo-cecal junction) 8. Procedure (charcoal tracing method) 7.II. measure the distance travelled by the charcoal meal (tracer) in the SI 9. Compute for the percentage distance travelled by the charcoal meal . dissect GIT of the mice.

Atropine A. . Drug class: Anticholinergic Antimuscarinic Parasympatholytic.III.

. depresses salivary and bronchial secretions Dilates the bronchi inhibits vagal influences on the heart Relaxes the GI and GU tracts inhibits gastric acid secretion prevents accommodation for near vision also blocks the effects of acetylcholine in the CNS. Atropine B.III. Mechanism of Action: Competitively blocks the effects of acetylcholine at muscarinic cholinergic receptors that mediate the effects of parasympathetic postganglionic impulses.

Results A. Formula: % travelled = Length of SI travelled by charcoal By charcoal meal Total length of SI .IV. Parameter that was Measured: Length of the small intestine travelled by the charcoal meal B.

45% 25-35% .IV. Results Computation: % travelled = 25 cm x 100 = 45.45 % By charcoal meal 44 cm Table 1: Effect of Atropine SO4 on GI motility % travelled by Charcoal Meal Normal values Atropine 45.

V. Discussion Atropine SO4 decreases the parasympathetic activity of all muscles and glands regulated by the PNS. Blockade of muscarinic receptors has dramatic effects on motility and some of the secretory functions of the gut. . This occurs because atropine SO4 is a competitive antagonist of the muscarinic acetylcholine receptors.

and intestinal transit time is lengthened. and both tone and propulsive movements are diminished. Therefore. Discussion However. . since local hormones and noncholinergic neurons in the enteric system also modulate GI function. GI emptying time is prolonged.V. In general. the walls of the viscera are relaxed. even complete muscarinic block cannot totally abolish activity in this organ system. GI smooth muscle motility is affected from the stomach to the colon.

This result could be possibly attributed to “mistiming” of the experiment. This result was above the expected values of 25 to 35%. Discussion In the experiment. . The group had a hard time feeding the specimen with the charcoal tracer.45% of the total length of the mouse’s small intestine. using Atropine SO4 as our drug. We started to time the 30 minute period after the completion of the 1g target dosage instead of timing it from the start of the charcoal feeding process. That is why it took us several minutes before we had fed the specimen with at least 1ml of the tracer. we have observed that the charcoal tracer has traveled 45.V.

VI. . primarily at muscarinic receptors. it decreases gastric motility. As a result. thus. It inhibits actions of acetylcholine at postganglionic parasympathetic neuroeffector sites. Conclusion Atropine sulfate is a potent parasympatholytic. it has a potent anti-diarrheal effect.

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