National Algal Biofuels
Technology Roadmap

MAY 2010

National Algal Biofuels
Technology Roadmap
A technology roadmap resulting from the National Algal Biofuels Workshop
December 9-10, 2008
College Park, Maryland

Workshop and Roadmap sponsored by the U.S. Department of Energy
Office of Energy Efficiency and Renewable Energy
Office of the Biomass Program
Publication Date: May 2010

John Ferrell Valerie Sarisky-Reed
Office of Energy Efficiency Office of Energy Efficiency
and Renewable Energy and Renewable Energy
Office of the Biomass Program Office of the Biomass Program
(202)586-5340 (202)586-5340

Roadmap Editors:
Daniel Fishman, Rajita Majumdar,1 Joanne Morello,2 Ron Pate,3 and Joyce Yang2

Workshop Organizers:
Al Darzins, Grant Heffelfinger, Ron Pate, Leslie Pezzullo,2 Phil Pienkos,4 Kathy Roach,5 Valerie Sarisky-Reed,2 and the
4 3 3

Oak Ridge Institute for Science and Education (ORISE)

A complete list of workshop participants and roadmap contributors is available in the appendix.

Suggested Citation for This Roadmap:
U.S. DOE 2010. National Algal Biofuels Technology Roadmap. U.S. Department of Energy, Office of Energy Efficiency and
Renewable Energy, Biomass Program.
Visit for more information

BCS, Incorporated 2Office of the Biomass Program 3Sandia National Laboratories

National Renewable Energy Laboratory 5MurphyTate LLC

2 of the Bulletin. this report could be “influential scientific information” as that term is defined in the Office of Management and Budget’s Information Quality Bulletin for Peer Review (Bulletin). 106-554) and information quality guidelines issued by the Department of energy. . the document was prepared in compliance with Section 515 of the Treasury and General Government Appropriations Act for Fiscal Year 2001 (Public Law No. As such. Further. This report has been peer reviewed pursuant to section II.This report is being disseminated by the Department of Energy.

DOE looks forward to continuing its work with diverse partners in evaluating renewable energy options and facilitating development of those that carry the greatest benefits today and in the years to come. Meanwhile. Prepared with the input of more than 200 scientists. this document represents the synthesis of the Biomass Program’s National Algal Biofuels Technology Roadmap Workshop. research managers. and demonstration (RD&D) projects that seek to tackle key technical hurdles associated with commercializing algal biofuels. These national challenges require near-term solutions as well as investments in nascent technologies that show promise for the future.Preface Welcome to the U. Through appropriated dollars and the American Recovery and Reinvestment Act of 2009. Biomass Program U. This Roadmap is intended to summarize the state of technology for algae-based fuels and document the research and development challenges associated with producing them at a commercial scale. while DOE works to deploy renewable energy and energy-efficient projects across the country today. as well as the recognition that we will not likely achieve these goals via one technology pathway. and supporting scientific literature. and the stability of our global climate. engineers. and other stakeholders. industry representatives. our economic wellbeing. Therefore. development. it remains committed to fostering technologies that could yield substantial benefits over time. environmentally sound biofuels. Department of Energy Preface  i . DOE has recently revived its investment in algal biofuels in response to the increased urgency of lowering greenhouse gas emissions and producing affordable.S. comments gathered during a public comment period. and the academic community are also increasing their efforts to optimize and commercialize this renewable energy source. sustainable algal biofuels will entail years of research and careful planning. It is intended to serve as a resource for researchers. Renewable energy lies at the intersection of some of our nation’s most pressing issues: our energy security. This Roadmap lays the groundwork for identifying challenges that will likely need to be surmounted for algae and cyanobacteria to be used in the production of economically viable. and we hope it fosters and informs participation from existing and new stakeholders as the next steps are taken to advance algal biofuels. engineers. private companies. DOE is investing in a variety of research. Achieving cost-competitive. reliable energy. Valerie Sarisky-Reed Acting Manager. and decision-makers by providing a summary of progress to date and a direction for future algae RD&D activities. but their significant potential to serve as renewable transportation fuels warrants our thorough consideration of what efforts are necessary to make them a commercial-scale reality. other federal agencies. Department of Energy (DOE) Biomass Program’s National Algal Biofuels Technology Roadmap.S.

which exposes the country to the risk of critical disruptions in fuel supply. dependence on foreign research both in EERE and other government agencies oil. a number of next generation have combined to capture the interest of researchers and biofuels show significant promise in helping to achieve entrepreneurs around the world. 5) production of both biofuels and valuable Energy Steven Chu announced the investment of $800M co-products. These include: the goal. The term algae can refer to microalgae. 2008.S. some microalgae and renewable source of biomass feedstock for biofuels have the potential to accumulate significant amounts has been explored previously. Photo courtesy of the our national security. creates economic and social uncertainties for businesses and individuals. a variety of different biofuels and gallons of renewable fuels. and 6) potential recycling of CO2 and other in new research on biofuels in the American Recovery nutrient waste streams. Renewable Fuel Standard (RFS) requiring transportation sugars from cyanobacteria and macroalgae. sustainable of lipids (more than 50% of their ash-free cell dry and commercially viable system has yet to emerge. biomass-derived fuels have received increasing attention as one solution to our nation’s continued and growing dependence on imported oil. fungible Workshop. cellulosic biofuels and biomass-based diesel. by 2022. President Obama and Secretary of wastewater). opportunities across the nation have renewed interest in developing algal feedstocks for biofuels production. Under certain conditions. while at the same time moving the nation ever closer to non-arable land. engineers. and impacts A culture of the microalgae Botryococcus. the end of the program. This announcement included funds for the Department of Energy (DOE) Office of Energy The DOE-supported Aquatic Species Program. meet the new RFS feedstock resources. to contain a minimum of 36 billion to fungible biofuels. The Energy Independence and Provasoli-Guillard National Center for Culture of Marine Phytoplankton Security Act of 2007 (EISA) established a mandatory (CCMP). biofuels derived from algae 1) high per-acre productivity. and macroalgae (or While the basic concept of using algae as an alternative seaweed). including starches and brought together more than 200 scientists. and of algae as a biofuel feedstock.S. and create economic and programs. and advanced biofuels. announcing $800 million in new biofuel research activities I n recent years. 3) use of otherwise non-productive. 2009. illustrated the potential Program to invest in the research. While cellulosic ethanol is expected to play a large role There are several aspects of algal biofuel production that in meeting the EISA goals. Much has changed since deployment of commercial algae-to-biofuel processes. including advanced and products can be generated using algae precursors. provide environmental benefits. 4) utilization of a wide variety of water energy independence. To accelerate the deployment of sources (fresh. weight).S. Of these candidates. development. ii Executive Summary  . Rising petroleum prices and a Additional funding is being directed to algae-to-biofuel national mandate to reduce U. These characteristics give great potential for The National Algal Biofuels Technology Roadmap an immediate pathway to high energy density.Executive Summary “ ” Developing the next generation of biofuels is key to our effort to end our dependence on foreign oil and address the climate crisis – while creating millions of new jobs that can’t be outsourced — Secretary of Energy Steven Chu at the White House ceremony on May 5. held December 9-10. marine. and Renewal Act. brackish. an effort Efficiency and Renewable Energy’s (EERE) Biomass undertaken from 1978 to 1996. 2) non-food based have the potential to help the U. saline. produced. In addition fuel sold in the U. a scalable. was convened fuels. cyanobacteria (the so called “blue-green algae”). The two-day event feedstocks and intermediates. These fuels can also be produced using other algae by DOE-EERE’s Biomass Program.

algal biomass production systems at commercial financiers. algal feedstocks and to document the feasibility and scalable. and regulators from across the country to scale.research managers. The ability techno-economic challenges associated with scaling to quickly test and implement new and innovative up of processes. this document will serve to commercial scale. By documenting the challenges across the algal discuss and identify the critical challenges currently biomass supply chain and highlighting research and hindering the economical production of algal biofuels at coordination needs and gaps. supporting scientific literature. lawyers. Executive Summary  iii . This document also seeks to explore technologies in an integrated process will be a key the economic and environmental impacts of deploying component to accelerating progress. development. guide researchers and engineers. and deployment efforts. The Roadmap In summary. research. federal agencies. and comments received during a public comment period. policymakers. and the private sector in implementing national This document represents the output from the Workshop. industry representatives. the Roadmap Workshop effort suggests document is intended to provide a comprehensive state that many years of both basic and applied science and of technology summary for fuels and co-products from engineering will likely be needed to achieve affordable. and sustainable algal-based fuels.

and sustainability of algae to biofuel systems. and hyper-saline environments to soil and in symbiotic associations with other organisms.FROM ALGAE FROM TO TO ALGAE BIOFUELS BIOFUELS An Integrated Systems An Integrated Approach Systems to Renewable Approach Energy to Renewable that is E nergy ALGAE FEEDSTOCKS CULTIVATION Microalgae and cyanobacteria can be cultivated via photoautotrophic methods (where algae require light to grow and create new biomass) in open or closed ponds or via heterotrophic methods (where algae are grown without light and are fed a carbon source. Open Ponds Example Cultivation Systems POLICY SITING AND RESOURCES iv Executive Summary  Development Systems and Techno-Economic Analysis: Guiding Path Toward the Research and a . and cyanobacteria (formerly called “blue-green algae”). macroalgae (seaweed). Isolating new strains directly from unique environments will ensure versatile and robust strains for mass culture needed in biofuels applications. brackish waters. Choices made for the cultivation system are key to the affordability. and taking advantage of the biology of algal strains selected for use in production systems is the foundation for processing feedstocks into fuels and products. Algae occur in a variety of natural aqueous and terrestial habitats ranging from freshwater. managing. Fermentation Tanks MICROALGAE CYANOBACTERIA MACROALGAE Algae as feedstocks for bioenergy refers to a diverse group of Closed Photobioreactors organisms that include microalgae. to generate new biomass). Designing an optimum cultivation system involves leveraging the biology of the algal strain used and inegrating it with the best suited downstream processing options. Understanding. marine. scalability. such as sugars. Macroalgae (or seaweed) has different cultivation needs that typically require open off-shore or coastal facilities.

. Affordable. 3) Processing of direct algal secretions. While lipids and carbohydrates are fuel precursors (e. These steps can be energy-intensive and can entail siting Conversion technology options include chemical. or a 2-O-C EXTRACTION combination of these approaches.g.. biochemical. and proteins. animal feed. optimizing extraction systems that consume less energy than contained in the algal products is a challenge due to the high energy needs associated with both handling and drying algal biomass as well as separating out desirable products.g. in water and can require process steps to concentrate 2) Extraction of algal metabolites. animal/fish feeds). issues. CH-O-C R2 O CH2-O-C R3 Bio-Crude Algal Lipid: Precursor to Biofuels Three major components can be extracted from algal biomass: lipids (including triglycerides and fatty acids). Algal cultures are mainly grown 1) Conversion of whole algal biomass. or harvested algal biomass prior to extraction and conversion. Most challenges in extraction are associated with the industrial scale up of integrated extraction systems. proteins can be used for co-products (e. gasoline. fertilizers. and surfactants) REGULATIONS AND STANDARDS Executive Summary  v Commercially Viable Algal Biofuel Industry . The end products vary depending on the conversion technology utilized. End Uses: • Biodiesel • Biogas • Renewable Hydrocarbons • Co-products • Alcohols (e. industrial enzymes. though these are also subject to a number of unique scale-up challenges. bioplastics. biodiesel and jet fuel). Focusing on biofuels as the end-product O poses challenges due to the high volumes and relative low CH2-O-C R1 values associated with bulk commodities like gasoline and O diesel fuels. and thermochemical processes.g. Abundant. While many analytical techniques exist.. carbohydrates. and Sustainable HARVESTING / DEWATERING CONVERSION Some processes for the conversion of algae to liquid Conversion to fuels and products is predicated on a basic transportation fuels require pre-processing steps such as process decision point: harvesting and dewatering. Some algal biomass production processes are investigating options to bypass extraction.

vi Executive Summary  .

.................................................. Contents 1.............................................................................................8 Isolation and Characterization of Naturally Occurring Algae ....................1 About the Roadmap................... 11 Photosynthesis and Light Utilization.....................................................20 Considerations of Genetic Modifications.......................................................1 America’s Energy Challenges............................................................... 29 Macroalgae.................................................................................................................................................................................................................................................. 16 The Genetic Toolbox....................................................................................................... 19 Applications of Biotechnology to Algal Bioenergy...................................................................................................................................................................................................................................................................................................................................................................................................1 Strain Isolation..................................10 2.............................................................7 References.....................................................................................................................................3 Early Work to 1996........................................... 16 Enabling Technologies: “Omics” Approaches and Bioinformatics.....................................................................................................................5 1............1 Cultivation Pathways......................................11 Carbon Partitioning and Metabolism.................................................................................7 Public-Private Partnerships..........................................................30 3......................................................7 2......................................................................2 Scale-Up Challenges...................................................................................8 2.............................................3 Algae-to-Biofuels: Opportunity and Challenges Today..................................................................................................................................................................................................................................................................................................................................................... Overview................. Algal Cultivation................................................................................................................................................ Screening and Selection ................................................................... 31 Contents  vii ................11 Algal Carbohydrates..............................................................................................1 1......................................................................8 Role of Culture Collections as National Algae Data Resource Centers.....2 A History of Domestic Algal Biofuels Development................................... Algal Biology...................................... 12 Lipid Synthesis and Regulation......................................................29 3............................................................... 15 2.....................................................................................................5 Regulations and Standards...........................................................................................................................................................................................3 Algal Biotechnology..........................................................................................................................................9 Selecting Algal Model Systems for Study.................................................................................3 1........................................ 31 Stability of Large-Scale Cultures ....................................... 12 Biohydrogen ................................................................................................. ..................... 23 3.............................................. 29 Microalgae and Cyanobacteria...................................................................................................................5 Technology and Analysis Challenges..........................2 Algal Physiology and Biochemistry..............................................................................................................................................................3 Research from 1996 to Present................................. 22 References............................................................................................................................................................................................1 Advantages of Algal Feedstocks..

.......46 6......................................................................................................................................................................................................................................................................................................................................42 5....................................................... 52 Supercritical Processing............................................................... 42 Organic Co-solvent Mixtures................................................................................................................................................................................37 Drying......................................................................................................................................................................................................................................................50 6........................................................................................................................... Conservation...................... 45 Heterotrophic Production....................................................................................................................................................2 Approaches for Macroalgae .................... 53 Chemical Transesterification....................................................................................................................................................................................................................... 38 4..............................................................e.........................................................................................................................................................................................................................................................44 Subcritical Water Extraction...................1 Approaches for Microalgae.....................................................................................................................................................................................50 Pyrolysis.................... Cell Rupture).....................................................40 Preliminary Look at Energy Balance ................................................ ...................................................44 Selective Extraction............................................................................................................................................................................................................................................ 32 Water Management................................................................................................................ 39 References.......................................................................................1 Current Practices for Lipid Extraction................................... 32 Nutrient Sources.............................. 38 Preprocessing..................................................................................................................................................................................................................... 53 Anaerobic Digestion of Whole Algae......................................48 Alcohols............................................................................................ Sustainability.......................... and Management.............................................................................................. 43 Accelerated Solvent Extraction.........................................................50 Gasification......................... 35 4........................................................................... 39 4................................................................................................................................................ 33 References..2 Processing of Whole Algae............................................................................................................................................................................................................. 45 Supercritical Fluid Extraction........................................................................48 6......... 53 6................. 42 Mechanical Disruption (i....... 37 4.............1 Direct Production of Biofuels from Algae................40 5................................................................................................................................................................................. 54 viii Contents  ..................................... and Recycling......................................................................2 Challenges.............................................................................................................................................................................................. 49 Alkanes...................37 Harvesting................................ System Productivity.............. 45 5.................................................................................................................................................................................................................................. 49 Hydrogen................ Extraction of Products from Algae ............................ 51 Liquefaction..............................3 Conversion of Algal Extracts................................................................................................ .................................................... Algal Biofuel Conversion Technologies .............................................. 38 Harvesting.................................................3 Systems Engineering .....46 References................................... Downstream Processing: Harvesting and Dewatering...........................................................................................................................................

............................................................. .......................... 70 Algal Blendstocks to Replace Middle-Distillate Petroleum Products....................................................................................75 Climate ....................................................................................................................................................................................................................... 55 Conversion to Renewable Diesel................................... 55 Catalytic Cracking...................75 Water............................ .................................................................................................................. 56 6................................................................80 Land...................................................................................................................................................................................................74 Photoautotrophic Macroalgae Approach...............................................................57 References..............................2 Commercial Products from Macroalgae.................................................................................4 Processing of Algal Remnants after Extraction......................................................................... 88 References.................................................................................... 83 Algae Production Techniques for Wastewater Treatment Plants............64 7...4 Integration with Water Treatment Facilities .......................... 61 7................ and Jet Fuel ................................................................................................................................................ 61 7.....................................................................................................................................................................................................................5 Co-location of Algal Cultivation Facilities with CO2-Emitting Industries.......................................................................................77 Carbon Dioxide...1 Resource Requirements for Different Cultivation Approaches........................................................................................................................................73 Heterotrophic Microalgae Approach.................. 88 Directions for Research and Development................................................................................................................................................................................................................. 70 Algal Blendstocks for Alcohol and Gasoline-Range Petroleum Products...................................................................................................................................................................................................................... 67 8.....................................2 Utilization..............71 References..............1 Distribution.64 References..........................................................1 Commercial Products from Microalgae and Cyanobacteria..............2 Resources Overview...................................................................69 8..............74 9.................. Resources and Siting ............................................................... Co-products ....................................................90 Contents  ix .........................................................................72 9................................................ Gasoline....................................................................................................... 86 9............................................................................................................................................................................................................. 58 7.............................................................. 69 8... 73 9.................................................................................................................................... Distribution and Utilization........................................................................................ 87 Barriers to Co-Location of Algae Production with Stationary Industrial CO2 Sources ........................................................... 73 Photoautotrophic Microalgae Approach...... 86 The Opportunity in Co-Locating with CO2-Emitting Sources...................... 84 Main Research Needs for Algae Production with Wastewater ................................................ 83 Wastewater Treatment and Recycling Applications... 81 9........................................................................................ Biochemical (Enzymatic) Conversion.........................................3 Potential Options for the Recovery of Co-products...............................................................................................

2 Components of Successful Public-Private Partnerships.................................... 104 10......................................................................................................... 104 10......................... 104 10..................110 11..........................................................................................................................10.......................... 109 11................ 105 11...............99 Engineering Analyses and GIS Assessments..................................................................115 Appendix 2: Contributors.............................110 11......................................5 Modeling Partnerships for Technology Development.................................99 Overview....7 Potential Directions for R&D Effort ...................................................3 Forums for Public-Private Partnerships......................................................... Public-Private Partnerships .............. 93 10................................................................................................................124 x Contents  ..................114 Appendices ................................................................................................................. Systems and Techno-Economic Analysis.......................................................................................................... 115 Appendix 1 : Workshop Participants....................................................6 Preliminary System Dynamics Modeling .................................................94 10.............................................................................. 111 References......................................................................3 Suggestions for TEA and LCA Approach (Workshop Results and Discussion).............................. 97 10...............................................................................................122 Appendix 3: Respondents to Request for Information on the Draft Roadmap...............................................................................................................................................................................4 Partnership Challenges in the Algal Biofuels Industry.........................................................................................................................................................1 The Benefits of Algal Biofuels Public-Private Partnerships.........................102 Life Cycle Analysis.........................1 Objectives of Techno-Economic Analysis .......................................93 10.............. 100 Impact of Geographic Variability of Inputs on Algal Biofuel Production Costs.................................................................................. 111 11........................................................ 109 11......................................................................................................................................................................................................................2 TEA Scope and Methods.....................4 Systems Analysis......................5 Algae Production Costs and Uncertainties..............

60% of 1. the United The framework for National Algal Biofuels Technology States is becoming increasingly dependent upon foreign Roadmap was constructed at the Algal Biofuels sources of crude oil. The which is used for producing transportation fuels. government and new energy technology research and development. the Energy Independence and Security Act (EISA) was enacted. and creates be found at http://www. energy supply for transportation liquid transportation fuels. including the Energy Independence and Security Act a 60-day public comment period was held to allow of 2007. the combustion of petroleum- Workshop was to gather the necessary information based fuels has created serious concerns about climate to produce an algal biofuels technology roadmap change from the greenhouse gas (GHG) emissions. held December 9-10. and guide RD&D investment in algal biofuels.1. the production of economically viable biofuels. as well as made provisions that Workshop. economic opportunities across the nation. and non-governmental organizations. the Program has undertaken a diverse portfolio of research. it lays for producing algal biofuels is regarded by many in the groundwork for identifying challenges that likely the field to be in its infancy and there is a considerable need to be overcome for algal biomass to be used in amount of RD&D is needed to achieve affordable. and industry. the state of technology the current status of algae RD&D. In doing so. reduces our dependence Roadmap. bioproducts and biopower. and criticisms not captured during the Workshop. While Biofuels derived from algae can meet these l onger-term addressing the potential economic and environmental needs of the RFS and represent a significant opportunity benefits of using algal biomass for the production of to impact the U. provides environmental benefits. which set new standards for More than 200 stakeholders were convened at the vehicle fuel economy. academia. at the University of Maryland-College Park.1). Despite their potential. The United States currently imports Technology Roadmap Workshop. and sustainable algal-based biofuels. Renewable Fuels Standard (RFS) mandate established within EISA.S. gallons of renewable fuels by 2022. with national laboratories.S. The RFS mandates blending of 36 billion This Roadmap presents information from a scientific. energy efficiency. and policy perspectives that can support billion gallons can be produced from corn-based ethanol. approximately two-thirds of its petroleum. that both assesses the current state of technology and provides direction to the Program’s RD&D efforts. agencies. Standard (RFS) that increase over time (Exhibit 1. the Roadmap describes fuels. The The legislation establishes production requirements for Workshop provided a stimulating environment to explore domestic alternative fuels under the Renewable Fuels topics affecting the development of algal biofuels industry.1 America’s Energy Challenges About the Roadmap As global petroleum supplies diminish. Algal biofuels offer great promise in contributing to Every attempt was made to ensure that the Roadmap the Program’s vision. as well as helping to meet the development process was transparent and inclusive. All comments are noted in the Appendix of this document. To accomplish its goals. The full proceedings of the Workshop can on oil.S. In 2007. energy security. of a viable. A major objective of the reserves. Overview The Workshop was able to capture the participants’ The Biomass Program (Program) of the U. Department experience and expertise during a series of technical of Energy (DOE) Office of Energy Efficiency and break-out sessions that spanned critical aspects of the Renewable Energy is committed to advancing the vision algal biomass supply chain and cross-cutting issues. The Program’s goals are driven by various federal policies and laws. The Workshop was organized by the Biomass Program to rising energy demand in many rapidly developing discuss and identify the critical challenges currently countries around the world is beginning to create hindering the development of a domestic. Furthermore. viewpoints. to the development of this Algal Biofuels Technology enhances U. representing a diverse range of expertise from promote the use of renewable fuels. Following the release of the initial draft of the Roadmap. intense competition for the world’s dwindling petroleum scale algal biofuels industry. scalable. academia. commercial. Overview  1 . development. in partnership fidelity and incorporate new information. industry. 1. the national laboratories. Workshop participants to evaluate the Roadmap for and deployment (RD&D) activities. sustainable domestic biomass industry that The outcomes from the Workshop provided key inputs produces renewable biofuels. of which only 15 economic.

0 2020 10.25 0.0 33. Potential oil yields from certain algae production of biofuels and bioproducts from components strains are projected to be at least 60 times higher than of biomass. microalgae have demonstrated potential oil yields that are significantly higher than the yields of oilseed crops Many pathways are currently under consideration for (Exhibit 1. For example.5 a 18.65 0.0 2.0 2023 b b b b a To be determined by EPA through a future rulemaking. Significant acreage and productivity will be required • Minimizes competition with conventional agriculture for biomass production to generate sufficient feedstock • Utilizes a wide variety of water sources to meet the RFS mandates.5 20. advanced biofuels must demonstrate GHG emissions UNIQUE ADVANTAGES OF ALGAL FEEDSTOCK FOR across their life cycle that are at least 50% less than GHG ADVANCED BIOFUELS emissions produced by petroleum-based transportation • High area productivity fuels. 2005).2 2013 1. approximately 15 times more productive to advanced biofuels such as high energy density fungible than jatropha.0 2021 13.95 12.0 a 11.0 a 21.1 2010 0.1 0.2).5 a 15.5 a 13.55 2014 1. The most promising among these are routes from soybeans.35 13.. b To be determined by EPA through a future rulemaking.15 2015 3.95 2012 0.5 1.0 15.6 11.0 a 5. oleaginous Exhibit 1. the study did not explore the potential of algae. However.75 18. while algae may co-products within biorefineries offer comparable biomass productivity as lignocellulosic feedstocks – the key biomass resource factored in the study.80 1.25 2017 5.0 billion gallons.75 16.0 a 2.0 26.0 24. offer significant advantages that complement traditional feedstocks towards these fuels. Cellulosic feedstocks were • Recycles stationary emissions of carbon dioxide identified by the Billion Ton Study as a significant source • Compatible with integrated production of fuels and of biomass (Perlack et al. 2009).0 2018 7. Overview  .1 Renewable Fuel Standard volume requirements (billion gallons) Cellulosic biofuels and biomass-based diesel are included in the advanced biofuel requirement. and approximately 5 times that of oil palm fuels for aviation and ground transport.75 a 3.25 a 7.5 0.0 36.5 2016 4. As required by EISA.0 2022 16.5 a 9. 2 1.0 30. CELLULOSIC BIOMASS-BASED ADVANCED BIOFUEL TOTAL RENEWABLE BIOFUEL DIESEL REQUIREMENT FUEL REQUIREMENT REQUIREMENT REQUIREMENT 2009 N/A 0. but no less than 1.Advanced biofuels face significant challenges in meeting the ambitious targets set by EISA. Algal biomass may per acre of land on an annual basis (Rodolfi et al.0 2019 8.0 28.95 2011 0.25 22..

The concept of utilizing the lipid stores as a source of • Algae can recycle carbon from CO2-rich flue emissions energy. Proposals to use algae as a means of producing energy fungible liquid transportation fuels. freshwater supplies. have been apparent since the mid-twentieth century. but the focus changed to Camelina 62 liquid fuels (biodiesel) in the early 1980s. and and 1960s indicated that starvation for key nutrients. Various reports during the 1950s • Algae can utilize waste water. gained serious attention only during from stationary sources.Advantages of Algal Feedstocks Early Work to 1996 Algae can be preferred feedstock for high energy density. Sunflower 102 studies of algal physiology and biochemistry. Techno-economic analyses Oil palm 635 and resource assessments were also important aspects of the program. A detailed engineering analysis by Benemann et al.500b of the project was completed (Sheehan et al. this idea ultimately became a major push of DOE’s Aquatic Species Program. pH. surges through the decade. The program lasted from 1978 until 1996 and supported research primarily at DOE’s National Exhibit 1.2 A History of Domestic and southeastern U.000-6. a comprehensive overview Algae 1. produced water. representing a diversity of aquatic Algal Biofuels Development environments and water types. genetic Jatropha 202 engineering. There are several started in the late 1950s when Meier (1955) and Oswald aspects of algal biofuel production that have combined to and Golueke (1960) suggested the utilization of the capture the interest of researchers and entrepreneurs around carbohydrate fraction of algal cells for the production of the world: methane gas via anaerobic digestion. a scalable. 1. inland saline habitats that typically The advantages of algae as a feedstock for bioenergy undergo substantial swings in temperature and salinity. and also for their ability not yet emerged. Many of the strains were isolated from shallow. The collection was narrowed approaches to the technologies being explored today. a Adapted from Chisti (2007) b Estimated yields. Advances were made through algal strain isolation and characterization. • Algal biomass is compatible with the integrated biorefinery vision of producing a variety of fuels and The Aquatic Species Program represents one of the most valuable co-products. Overview  3 . dates back to the 1940s. primarily green algae (Chlorophyceae) and diatoms (Bacillariophyceae). northwestern. The isolates were screened for their tolerance to variations Although. 1998) was invested during the 18-year Soybean 48 program. comprehensive research efforts to date on fuels from microalgae. In 1998. thereby reducing competition for limited as nitrogen or silicon. this report The Aquatic Species Program researchers collected more than 3. including power plants and the oil embargo of the early 1970s and the energy price other industrial emitters. with projected costs for fossil fuels. to the 300 most promising strains.000 strains of microalgae over a seven-year period from various sites in the western. (1978) indicated that algal • Algal productivity can offer high biomass yields per systems could produce methane gas at prices competitive acre of cultivation. The Aquatic Species OIL YIELD Program also funded research at many academic CROP (GALLONS/ACRE/YR) institutions through subcontracts. formerly the Solar Energy Research Institute). process development. and temperature. During the early years. Approximately $25 million (Sheehan. The discovery that • Algae cultivation strategies can minimize or avoid many species of microalgae can produce large amounts of competition with arable land and nutrients used for lipid as cellular oil droplets under certain growth conditions conventional agriculture. earlier studies have laid foundational to produce neutral lipids.. however.S. commercially viable system has in salinity. 1.2 Comparison of oil yields from biomass feedstocksa Renewable Energy Laboratory (NREL. the emphasis was on using algae to produce hydrogen. such saline water. and demonstration- scale algal mass culture. Some of the highlights are described briefly below. could lead to this phenomenon. 1998).

conversion of lipids to gasoline) species in the collection were shown to accumulate as were evaluated (Milne et al. harvesting via flocculation even with optimistic assumptions about CO2 credits and was deemed particularly encouraging (Sheehan et al.or methyl-esters (biodiesel) was overall rate of oil production in a culture (because of the successfully demonstrated in the Aquatic Species concomitant decrease in the cell growth rate). However. 2007). In this species. Different cost process step that is highly energy. estimated costs for unextracted 1998). ACCase activity was found to increase deep). Inc..S. Of particular note was the Outdoor the cell wall) is a trigger for increased oil production. cost. 1999. Cost-effective methods of harvesting the necessity for very low-cost culture systems. This catalyzes the first step in the biosynthesis of fatty acids used facility utilized two 1.and capital-intensive. scale-up. but lower oil producing. was the focus demonstration-scale outdoor microalgal cultivation of many of the biochemical studies. 1989). primarily triacylglycerides (TAGs) (Chisti. but no further fuel much as 60% of their dry weight in the form of lipid. 1998). primarily in C.$186 per purification of the oil are also cost-intensive steps. issues associated with such methods. and conversion to fuel are critical to successful In addition. it became clear that novel solutions would be of aquifers. Harvesting is a the single largest influence on fuel cost. chrysolaminarin saline water. Extraction of oil droplets from the cells and algal oil were determined to range from $59 .After promising microalgae were identified. A Test Facility in Roswell. an oleaginous diatom. purification. Under Aquatic Species Program subcontracts. 1988) with the hope of eventually turning down the Sufficient resources were identified for the production flow of carbon through these pathways. 1990). These initial to be more than 90% with careful pH control. productivity improvements. Under inducing conditions.. and environmental lipid accumulation under conditions of nutrient stress.000 m2 outdoor. Sheehan et al. cryptica were characterized (Roessler. such and dewatering algal biomass and lipid extraction. stable production of algal successful transformation of microalgae (Dunahay et al. depth research. the commercially viable strains for oil production.. strains from the wild. In addition.. production. Conversion of Although nutrient deficiency actually reduces the algal oils to ethyl. 1988). Detailed techno-economic analyses underlined process steps. it became possible to manipulate the expression of utilization (bubbled through the algae culture) was shown ACCase in an attempt to increase oil yields. shallow (10-20 cm for TAG synthesis. smaller ponds for inocula production. Hawaii. paddlewheel-mixed raceway ponds.. and the efficiency of CO2 1995). as biosynthesis of these compounds competes for fixed carbon units that might otherwise be used for Several resource assessments were conducted under the lipid formation. the Roswell area. petroleum consumption. plus several under the nutrient stress conditions (Roessler. Cyclotella cryptica. Studies focused on suitable land.. 1990). characterization. analyses led to differing conclusions on fuel cost.. operated by key enzyme is acetyl-CoA carboxylase (ACCase). 1996). however. costs of these resources can vary widely depending During the course of the Aquatic Species Program upon such factors as land leveling requirements. growth was conducted in California. Low attempts at metabolic engineering identified a pathway to nighttime and winter temperatures limited productivity in modify the gene encoding in the ACCase enzyme. 1987 and in desert regions of the Southwest (Maxwell et al. suggesting that the of the Aquatic Species Program in 1996 halted further technology could have the potential to have a significant development of these potentially promising paths to impact on U. but studies examined the ability of many strains to induce failed to fully address the scale. New Mexico. biosynthesis of the storage carbohydrate. (Weissman et al. but overall biomass productivity averaged no effect was seen on lipid production in these preliminary around 10 g/m2/day with occasional periods approaching experiments (Jarvis et al. biomass was demonstrated. 50 g/m2/day. as unlined open ponds (Benemann and Oswald. and other needed for biological productivity and various problematic issues. and New Mexico under conditions of insufficient silicon (a component of (Sheehan et al. and thus the enzyme was extensively University of California-Berkeley. Overview  .g. distance from CO2 point sources. further Aquatic Species Program focused on solvent systems. biological productivity was shown to have commercialization of the technology.. and CO2 resources (power plants). enzymes involved in the Aquatic Species Program. studying Program and shown to be one of the less challenging this response led to valuable insights into the mechanisms aspects of the technology.. The 4 1. but Among various techniques. The termination of many billions of gallons of fuel. With the advent of the first successful in that long-term. 1985). The raceway design suggesting that it may play a role as a “spigot” controlling was based on the “high rate pond” system developed at lipid synthesis. which Microbial Products. 1998). or engine testing was carried out. The systems were characterized (Roessler. some process options (e. other biofuel of lipid biosynthesis. One serious problem encountered was that the desired starting strain was often outgrown by faster Additional studies focused on storage carbohydrate reproducing. For example.

the Aquatic Species Program was successful in demonstrating the feasibility of algal culture as a source of oil and resulted in important advances in the technology. needed to support commercialization activities. such as a major Defense Advanced Research Projects Agency solicitation. Overview  5 . In contrast to the development of cellulosic biofuels which benefit from a direct agricultural and process Research from 1996 to Present engineering A sizable and strategically structured investment from DOE. In particular. macroalgae for biofuels and beneficial re-use of CO2. DOE sponsored the Marine Biomass 1. Similar to the findings of the Aquatic lifeblood of the burgeoning biofuels industry today. Detailed results from the Aquatic Species Program research investment are available to the public in more than 100 electronic documents on the NREL Web site at www. federal agencies. cyanobacteria. and the Department of Agriculture. funding levels are beginning to increase. and Biomass Program are all funding research activities By reviewing the technology gaps and cross-cutting needs. the National to tackle the challenges of algal biofuels is thus Science Foundation.3 Algae-to-Biofuels: Program. which was trading at less than $20/barrel in 1995. increasingly focusing on algal biofuels research. Additionally. Recent initiatives. and the private sector in implementing a nationally coordinated effort toward Additionally. State funding programs and research support from private Technology and Analysis Challenges industry also make up a significant proportion of research funding. a number of U. 1. national labs are developing a viable and sustainable algal biofuel industry. Also from 1968-1990. Courtesey of CCMP. researchers concluded that algal-derived Algae must be considered as part and parcel of the SNG would not be cost-competitive with fossil fuel gas. Further. significantly outpacing government funding. Cyclotella cells.nrel. 1987). and the Roadmap aims to guide researchers and engineers. and sustainable feedstocks are the California kelp. 1998). and several DOE Small Business Innovative to reduce the level of risk and uncertainty associated with Research (SBIR) request for proposals. affordable. cost-effective solutions for the culture and process engineering challenges. from algal feedstocks and to document the feasibility and techno-economic challenges associated with commercial scaling up of processes. that include investigating microalgae. it was Office of Scientific Research (AFOSR) algal bio-jet determined that a great deal of RD&D is still necessary program. Overall. the work highlighted the need to understand and optimize the biological mechanisms of algal lipid accumulation and to find creative. particularly to substitute natural gas Today (SNG) via anaerobic digestion (Bird and Benson. it also became clear that significant barriers would need to be overcome in order to achieve an economically feasible process.barrel (Sheehan et al. a research initiative to determine the technical and economic feasibility of macroalgae cultivation and Opportunity and Challenges conversion to fuels. federal funding for algal research has come scale.. Office of Fossil Energy. However. Private investment in algal biofuels This Roadmap seeks to lay down the first comprehensive has been increasing at a dramatic rate over the last few state of technology summary for fuels and co-products years. policymakers.S. Office of Science. suggest that the algae-to-biofuels process so it can be commercialized. there is no parallel agricultural Since the end of DOE’s Aquatic Species Program in enterprise equivalent for cultivating algae at a similar 1996. and public-private partnerships. feedstock mix for producing advanced biofuels. It was concluded that algal biofuels would not be cost-competitive with petroleum. the Air Force Based on the information provided at the Workshop. regulations and standards. Species Program. Primary efforts were focused on open ocean culture of Abundant. these activities must be accompanied with DOE’s Advanced Research Projects Agency-Energy conducive developments on the non-technical fronts – (ARPA-E). the Department of Defense.

and nutrient resource requirements for siting of microalgae Resources (heterotrophic & photoautotrophic) and macroalgae production systems and Siting • Integrate with wastewater treatment and/or CO2 emitter industries (in the case of heterotrophic approach) • Address salt balance. water. heterotrophic. and mechanized seaweed harvesting) Harvesting and • Minimize process energy intensity Dewatering • Lower capital and operating costs • Assess each technology option in terms of overall system compatibility and sustainability • Investigate multiple approaches (e. direct fuel production. open. subcritical water. dissolved air floatation. fertilizers. engine performance and material compatibility) • Assess and characterize land. and nutrients • Identify and address environmental risks and impacts • Investigate multiple harvesting approaches (e..g. microwave. and anaerobic digestion) Fuel • Improve catalyst specificity. and thermal management PURSUING STRATEGIC R&D: TECHNO-ECONOMIC MODELING AND ANALYSIS Given the multiple technology and system options and their interdependency. closed. sonication. weather impacts.g. energy balance. phototrophic.g.g.g. and materials from algal remnants (e. sedimentation. such as operational temperature. hybrid. and durability Conversion • Reduce contaminants and reaction inhibitors • Minimize process energy intensity and emissions over the life cycle • Achieve high conversion rates under scale-up conditions • Identify and evaluate the co-production of value-added chemicals.g. carrying capacity. including quality and safety trials to meet applicable standards • Characterize algal biomass. and surfactants) Co-products • Optimize co-product extraction and recovery • Conduct market analyses. activity. and secretion) Extraction and • Achieve high yield of desired intermediates. biogas. high-throughput screening technologies Algal Biology • Develop open-access database and collections of existing strains with detailed characterization • Investigate genetics and biochemical pathways for production of fuel precursors • Improve on strains for desired criteria by gene manipulation techniques or breeding FEEDSTOCK • Investigate multiple approaches (i. energy. flocculation. water & nutrient reuse.. side reactions. biofuel. and end-product variability and Utilization • Optimize distribution for energy and costs in the context of facility siting • Comply with all regulatory and customer requirements for utilization (e. preserve co-products Fractionation • Minimize process energy intensity • Investigate recycling mechanisms to minimize waste • Address scaling challenges. bioplastics. and coastal/off-shore systems. filtration.. an integrated techno-economic modeling and analysis spanning the entire algae to biofuels supply chain is crucial in guiding research efforts along select pathways that offer the most opportunity to practically enable a viable and sustainable algae-based biofuels and co-products industry. animal/fish feeds. centrifugation. industrial enzymes. FEEDSTOCK and mixotrophic growth) Algal • Achieve robust and stable cultures at a commercial scale Cultivation • Optimize system for algal productivity of fuel precursors (e. OVERCOMING BARRIERS TO ALGAL BIOFUELS: TECHNOLOGY GOALS PROCESS R&D CHALLENGES STEP • Sample strains from a wide variety of environments for maximum diversity • Develop small-scale. and bioproducts under different storage and transport scenarios for INFRASTRUCTURE INFRASTRUCTURE Distribution contamination. water.. energy. supercritical fluid.. selective extraction. and separations CONVERSION CONVERSION • Investigate multiple approaches to liquid transportation fuels (e. intermediates. 6 1. solvent systems. thermochemical/catalytic conversion. lipids) • Sustainably and cost-effectively manage the use of land. biochemical conversion. stability. climate..e. pressure.. Overview  .

& Nagle. 179–183. Microbiol.D. such as research and development process to help ensure algae intellectual property rights. RD&D activities can inform further development of applicable laws and standards. & Ohlrogge. Appl. 1605(UC-61).. 1.. Chisti. Adv. 1990. Dunahay.J. with shape-selective zeolites. supporting education are legally and safely developed and the end-products will be critical to create intellectual talent and the (i. T. 11. J. R.. W. regulation. 1978. However. 21(3).. Journal of Phycology. Roessler. E. it is recognized economic. Roessler. Engineering Design and Cost Analysis of a Large-Scale Microalgae Biomass System. there are no existing standards for various aspects of algal biofuels production. 1004–1011. NTIS# H CP/ T. structuring public- biofuel developers need to foresee and understand the private partnerships for successful ventures is a challenge potentially applicable legal requirements early on in the in itself given the myriad issues and interests.. P. Being a nascent industry.R. Overview  7 . 223–242. 1993.e. P. Meier.US Patent 5928932 Abstract. Available at: [Accessed November 3. 2007. Algal from multiple entities. W. J. Biomass as feedstock for a bioenergy and bioproducts industry: the technical feasibility of a billion-ton annual supply.E. P. Perlack. P.. N.. 1995. 2009]. Journal of Biological Chemistry. 1988. 1955. Evans. ORNL/TM-2006/66. References Benemann. et al.. Catalytic conversion of microalgae and vegetable oils to premium gasoline. 219-232. 2008. et al. Jarvis.G. P. 100–112.Regulations and Standards Public-Private Partnerships While the Roadmap’s primary objective is to highlight A collaborative framework of public-private partnerships the technical challenges and opportunities associated offers an opportunity to jointly address the technological. Biomass. Microalgae for oil: strain selection. Biodiesel from microalgae. 91.G.G.. Phycol.B. Rodolfi. 2005.J. Biotechnology Advances. and policy. E. L. Jarvis... 102(1).. biofuels and co-products) comply with applicable workforce needed to allow this industry to grow. R. & Roessler. Oak Ridge National Laboratory. However.. Effects of silicon deficiency on lipid composition and metabolism in the diatom Cyclotella cryptica. & Roessler. 1999.A. Biological cycles in the transformation of solar energy into useful fuels.. and policy and regulatory challenges as that RD&D activities need to be carried out under a resolution of these issues will likely require participation framework of standards. Biological transformation of solar energy. Genetic transformation of the diatoms Cyclotella cryptica and Navicula saprophila. T. Solar Energy Research.G. TN. consumption standards. Pursoff. Isolated gene encoding an enzyme with UDP-glucose pyrophosphorylase and phosphoglucomutase activities from Cyclotella cryptica .L. 24. induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor. Oak Ridge. Biotechnol Bioeng. Milne. Cloning and characterization of the gene that encodes acetyl-coenzyme A carboxylase in the alga Cyclotella cryptica. 394–400.. R. J. 25(3). C. Final Report to the US Energy Department.G. Y. with algal biofuels commercialization. 294–306.. 31(6). Also. & Oswald.J. & Golueke. 1960. Oswald.

Because it is not yet known how algae will be cultivated on a mass scale. rivers. within an aqueous habitat. (seaweeds). actual strain identification should be based on The goals of algae isolation and screening efforts are to molecular methods such as rRNA sequence comparison. For large-scale sampling and isolation efforts.. versatility possible. 2. the development and hyper-saline environments. distribution but also for the temporal succession brought about by seasonal variations of algae in their habitats. and soil (Round. In all cases. Geographic Information Systems (GIS) data and and carbohydrates. 2004). Furthermore. ponds. Planktonic algae may be used photosynthesis and live in aquatic habitats.or eukaryotic. 1984). products. salinity. The exact screens employed would depend geothermal springs. Algal Biology The term “algae” commonly refers to a diverse mix of Additionally. 2005). be isolated by traditional methods (for a comprehensive review of algal culturing techniques. Collection sites and would provide fatty acid profiles.2. and cyanobacteria (historically known as traditional cultivation techniques may be used such as blue-green algae). The selection of specific locations can be determined by sophisticated site Screening for metabolite production may involve selection criteria through the combined use of dynamic determining the cellular composition of proteins. oceans. of automated systems that provide information regarding Furthermore. high-throughput automated isolation techniques involving Screening and Selection fluorescence-activated cell sorting (FACS) have proven to be extremely useful (Sieracki et al. which includes fresh. lipids. marine require significant experimental effort. tolerance to environmental variables (temperature. a helpful screen for oil production would terrestrial environments in a variety of geographical allow for distinction between neutral and polar lipids. Because all of these parameters habitats ranging from freshwater to brackish water. Algae include Isolation Techniques microalgae (unicellular eukaryotic organisms). whereas benthic algae may single or multi-cellular and pro. and though find application in biofilm-based production facilities. 8 2. and new approaches are needed to develop Sampling strategies should not only account for spatial screening methods for extracellular materials. Algae can be in suspended mass cultures. and generation. oxygen levels. questions medium. Because of Isolation and Characterization of morphological similarities when comparing many algal Naturally Occurring Algae species. coordinated to ensure broadest coverage of environments and to avoid duplication of efforts.e. streams. 2005). they typically live in aquatic environments and are capable of photosynthesis. typically found in planktonic (free floating) and benthic algae have been unified based on their ability to carry out (attached) environments. large-scale sampling efforts should be all parameters simultaneously would be helpful. locations to maximize genetic diversity. Algal Biology  . hypersaline. some algal strains take weeks to months to affect every step of the algae to biofuels supply chain. on the cultivation approaches and fuel precursor desired. this is not always the case. lakes. macroalgae For isolation of new strains from natural habitats. Ecosystems to be sampled could include the organism regarding metabolites useful for biofuels aquatic (i. Due to their diverse characteristics. see Anderson. and Algae can be isolated from a variety of natural aqueous nutrient requirements. other gene markers.1 Strain Isolation. or identify and maintain promising algal specimens for in the case of closely related strains. metabolite production.. The term growth physiology encompasses a number of parameters such as maximum specific growth rate. and strain robustness. type and strain of algae being cultivated will ultimately However. pH. Traditionally. Natural Habitats maximum cell density. can include public lands as well as various sites within many strains also secrete metabolites into the growth our national and state park systems. brackish. Screening Criteria and Methods new strains should be isolated from a wide variety of An ideal screen would cover three major areas: growth environments to provide the largest range in metabolic physiology. and measuring the productivity of analysis tools. the enrichment cultures (Andersen and Kawachi. CO2 levels). maps. acidic and alkaline environments) and For example. cultivation and strain development. algae are organisms from different kingdoms of life. Some of these could prove to be valuable co- of ownership of isolated strains should be considered.

) (Bligh and Dyer. germination conditions cryopreservation. valuable strains. et al. Omics data (Genomics. and phylogeny either by themselves 8. Mutants biology. Environment and strain history (specific habitat. Some minimal growth information is available from includes parameters such as culture consistency. To obtain versatile and robust strains common method for determination of lipid content in algae. Strain administration (number in collection.000 strains. toxicity) Currently... However. They may also 7. only a few major collector) algal collection centers exist in the United States and 4. It could also reduce dissemination of detailed information about potentially the number of redundant screens of algal species. but it is very difficult to obtain resilience. with technologies is an important step in enabling the testing about 3. or in connection with outside collaborators. metabolites. but it requires a significant quantity of biomass (Ahlgren et it would be prudent to consider the isolation of new. Practical applications (general and industrial) information on the characteristics of currently available 10. and provide 3. that can be used for mass culture in biofuels applications. Growth conditions (media. For both direct using lipid-soluble dyes have also been described. pH) & support research on determining strain characteristics. The function of a culture collection often 6. National Center for Culture of Marine Phytoplankton at the Bigelow Laboratory for Ocean Sciences in West Boothbay At this time. algal strains. The culture collections such as UTEX (The Culture Collection development of small-scale but high-throughput screening of Algae at the University of Texas at Austin. de la Jara et al. algae stems from a lack of high-throughput methodologies because many of the strains in these collections have been that would allow simultaneous screening of multiple cultivated for several decades. community stability. Previous robustness of particular existing strains. Information could include: 1. they could potentially are rapid screening procedures necessary for the biofuels serve as nuclei for the development of national algae field. Therefore. Proteomics. temperature. Strain properties: Cytological. open access repository could accelerate do not always perform similarly in outdoor mass R&D of algae-based biofuels production systems. such as growth rate and metabolite properties. molecular. it is also industry has further exacerbated the flow of relevant strain important to consider the strain’s robustness. some may have lost original phenotypes. and breeding and metabolic engineering approaches to improve though these methods require much less biomass (as little biofuels production. 2003). taxonomy. which data. Transcriptomics. biochemical. At present. and susceptibility to more detailed information on growth. basic research resources. The establishment studies revealed that algae strains tested in the laboratory of a central strain. and predators present in a given environment. Role of Culture Collections as National reference) Algae Data Resource Centers 2. subspecies name. but could prove extremely useful for the identification resource centers. 1998).500 strains. 1991. 1959). Protection of intellectual property in private or Metabolomics) 2. Culture collections are necessary to preserve the diversity preservation) of natural habitats. Culture collection organizations of species (particularly in mixed field samples) necessary could be responsible for the gathering and for the future of algal ecology. other countries. Not only document data on strains. is the most nutrient requirements. Fluorescent methods strains directly from unique environments. small-scale simulations of mass A number of algal strains are currently available from culture conditions will need to be performed. Plasmids and Phages transcends simple depository functions. Maine). 2007. for example. and CCMP (The Provasoli-Guillard of hundreds to thousands of different algal isolates. They currently maintain thousands & screening results of different algal strains and support the research and industrial community with their expertise in algae 5. pathogenicity. it has not yet been established if these variety of algae for assembly into a culture collection that methods are valid across a wide range of algal strains (Elsey will serve as a bioresource for further biofuels research. cultures (Sheehan et al. no central database exists that provides global 9. Solvent extraction. protect genetic material. to determine a strain’s robustness. it will be important to isolate a wide as a single cell). Strain name (species. Further improvements in analytical methodology could be made through the As the major culture collections already collect and development of solid-state screening methods. Texas).. existing culture collections. native al.For mass culture of a given algal strain.. Biological interaction (symbiosis. Algal Biology  9 . such as mating capability or versatility regarding productivity. the bottleneck in screening large numbers of Harbor. with more than 2.

cost commodities such as lysine and ethanol. which will necessitate the use or strains with characteristics useful for large-scale of closed bioreactors. 2006). The ability of an algal species to secrete fuel precursors may be attractive because it could reduce or skip the cell In relation to biofuels. transported into the cell (Ceron Garcia et al. to providing information on basic cellular processes then collection of the atmosphere above the culture will regarding the synthesis of fuel precursors. For example. source. If the carbon source is utilized by the cell. Resistance to predators and be attractive attributes of algal strains.. intermediate. Finally. Even that other strains will be chosen for production. but we cannot be certain that laboratory strains unfavorably alter fluidics properties or provide a carbon and production strains will be sufficiently related to allow source favoring growth of contaminants. A reasonable first approach to identify model species that are optimal for the production of a desired fuel is through a survey of the literature. or methane (via anaerobic that are currently deployed worldwide at massive scale to digestion). However. carbohydrates. Species with sequenced genomes and transgenic secretion actually makes the product more readily capabilities are the most amenable to investigating cellular available. viruses could also be a useful phenotype. if the desired product is volatile. of either intermediates or products into the growth medium could make these compounds vulnerable to contaminating Useful Algal Characteristics microbes for catabolism. Also to be considered is whether growth. there are two types of algal model harvesting step. they are still associated with the cells in a lipid strains that grow well in the laboratory are suitable for biofilm matrix. Co-products could include pharmaceuticals manufacture everything from cancer drugs to high. Other traits like the ability to grow to Mixotrophic Growth high cell density in continuous culture may allow a strain to be maintained while at the same time reducing the amount Heterotrophic or mixotrophic growth capabilities may of water to be processed daily. contamination by undesired microbes living off the carbon Possible fuel types of interest could include H2. 1998). this is not generally a problem with well- isoprenoids. Rapid growth is important both for overall productivity and the ability to compete with Capability for Heterotrophic or contaminating strains.g.. there may be practical problems systems to consider studying: species or strains amenable to consider. supplements. and high cell densities can be achieved. cellular metabolism is already geared toward production. For example. Algal Biology  . even under mixotrophic agents could reduce the costs of harvest as long as it could conditions where the substrate is not known to be be controlled to avoid settling in the cultivation system.Selecting Algal Model Systems that naturally make abundant amounts of the desired for Study product. an abundance of exported lipids could feasible. it to secrete long-chain hydrocarbons (e. or co-product to be produced. in a practical sense. so it is possible hydrocarbon phase in solution (Banerjee et al.volume/ (therapeutic proteins and secondary metabolites). a large number of model and possible strain development for production. 2002). In some species. However. or materials for nanotechnology (in the case of the silica cell wall of diatoms). 2006). Pilot-scale experimentation and further metabolic engineering is required to evaluate Culture stability over long periods will be a key to low cost possible advantages and disadvantages of secretion. the number of algal systems that can be studied in depth has to be limited because a critical mass of researchers Secretion of Products or Intermediates is required to make progress on a given species. which simplifies characterization Given the diversity of algae. and species be necessary to isolate it. alcohols (either directly or established fully-heterotrophic fermentation technologies through biomass conversion). such as.. systems could be studied. Botryococcus was shown in the Aquatic Species Program that not all braunii). and thus are not free to form an organic large-scale culturing (Sheehan. growth in both Targeting Desired Fuel Product or Intermediate light and dark periods is possible. food low. However. Adapting if sustainable secretion could be achieved. However. it is not clear the lessons learned from laboratory model strains to what the effect of a lipid emulsion in an algal culture would strains known to be capable of large-scale growth may be be. or a screen of environmental isolates for species 10 2. production of biofuels. secretion for lessons from the former to be applied to the latter. A potential disadvantage of the addition One consideration in choosing model systems is the of external carbon sources is the possibility of increased type of fuel. Also the ability addition of supplemental carbon results in increased lipid to flocculate without addition of chemical flocculating accumulation (Xu et al. lipids. although there are algae known processes since the basic tools are in place. In such a strain.

algae. Certain microalgal species also naturally the efficiency of light utilization (Polle et al. accumulate large amounts of TAG (30-60% of dry weight). and potentially. algal photosystems have built-in More recently. 1994.. there remains be inter-convertible. starch transiently accumulated culture at greater than laboratory scale is not utilized.e. carbon partitioning in algae is less understood biomass productivity values in the range of 100-200 g/ and research on how algal cells control the flux and m2/day have been presented (Chisti. 2003. although concentrations approaching 20% lipid have been reported Carbon Partitioning and Metabolism in some species (Chu et al.2 Algal Physiology strategies to prevent the over-absorption of light energy. 2008. proteins. biomass 2007. Schwender et al. 1994. 2008). the percent of biomass and Fernie. It may be useful to carry out photosynthetic of carbon between alternative storage products will efficiency studies in algae similar to those that have be important for metabolic engineering of algae. 2008). Ruuska tend to absorb all the incoming light.. however. A fundamental understanding of both cultivation process design and strain improvement ‘global’ regulatory networks that control the partitioning projects. 2006.. and Niyogi.. some microalgae accumulate lipids In an effort to overcome this barrier. 2005). Research has also When algae are cultivated photosynthetically. photoinhibition or the reduction O2 and organic macromolecules such as carbohydrates of photosynthesis due to light damage can still occur and lipids. that is the desired fuel precursor. Foyer et al. Cyanobacteria and macroalgae. a link between starch and lipid metabolism has There are many good reviews available that cover basic been established. In young Arabidopsis seeds The majority of light that falls on a photosynthetic algal and Brassica embryos. preventing it from et al.. 2007). It is.. in their productivity. Theoretical productivity is an important concept. a potentially important implication limitations of algal photosystems regarding light utilization. It is. and shares the Eberhard et al. and exhibit photosynthetic efficiency and lipid production There is still much to learn about the dynamics and at least an order of magnitude greater than terrestrial crop regulation of the photosynthetic apparatus (Eberhard et al. A large majority of and Biochemistry absorbed incident light is dissipated as heat and could be Many algae are photosynthetic organisms capable of considered “wasted.1). Starch is a common carbon and energy algal photosynthetic processes (Nelson et al. Algal Biology  11 . 1994. there is no partitioning of photosynthetically fixed carbon into various current consensus on the true maximum productivity of groups of major macromolecules (i. 1999).. 2004. More emphasis should be placed on understanding a general rule. 1994. 1991). Nelson and Yocum. 2007). Libourel and Shachar-Hill. carbohydrates.. and predictive Photosynthesis and Light Utilization modeling (Vemuri and Aristidou. been carried out for plants (Zhu et al. Even when compound and its synthesis precedes oil accumulation. 2009.. affecting growth rate. Regardless of the cultivation practices used 2. the been done in plant systems to understand carbon flux in efficiency of photosynthesis is a crucial determinate biosynthetic and degradative pathways (Lytovchenko et al. storage compound in plants and algae. for biofuel production. Lipids and carbohydrates.. knowledge of metabolic pathways. are all potential biofuels lipids and/or carbohydrates could be very useful for biofuels or biofuel precursors..2.” Even with this photoprotection. nutrient starvation. exposed to high light. 2007 and 2008) Further... as 2008). under harvesting solar energy and converting CO2 and water to certain light regimes. Krause and same precursors with the storage lipid TAG (Exhibit Weis. possible that TAG and starch could to maximize light exposure (see Chapter 3). total dry weight (Mcdermid and Stuercke. Under stress conditions such as high light or (Long et al. because it can be used to set achievable goals for 2009. important to strain development and designing cultivation strategies. In and starch metabolism was most active before the lipid high cell density cultures. 2003). studies in higher plants showed that when 2. therefore. cells nearer to the light source accumulation phase (Kang and Rawsthorne. which can lead to oxidative damage. 2005.. Yang et al. it was shown that such as triacylglycerols (TAG) as their main carbon storage reducing the size of the chlorophyll antenna can increase compounds. 2002). Allen et al. Mcdermid and Stuercke. with lipid these processes if we are to better engineer the capture accumulation in macroalgae typically being less than 5% of and utilization of light energy for biomass production. and lipids) is critically needed (Boyle and Morgan. accumulate mostly carbohydrates. pathway analysis. 2003). Metabolic networks have been reconstructed in various microbes from genomic and transcriptomic data.. Sweetlove production. plants (Hu et al. indicating that starch is an important storage reaching more distant cells (Christi. therefore. understand the metabolic pathways and processes that Understanding carbon partitioning will require extensive generate them in order to advance biofuels production. along with biologically Knowing how and when carbon is partitioned in a cell into produced hydrogen and alcohols. 2002). 2002). Though theoretical However.

which are important cellulose-based cell walls and accumulating starch as potential fuel precursors. (Roessler. Several formation of TAG in plants first involves algae commonly use starch for energy storage. There are also many algae that completely lack 2000. Athenstaedt and Daum. Cell walls can also be a technical barrier. but like those from plants. Microorganisms capable of fermenting laminarin and TAG biosynthesis in algae has been proposed to occur mannitol from Laminaria hyperborea to ethanol have via the above Kennedy pathway described in plants. or efficiently extracting biofuel precursors of TAG (Hu et al. transferred from CoA to positions 1 and 2 of glycerol- for example alginate found in many brown algae. use 16 or carbohydrates such as laminaran. Other algae. 2000). Some algae.. the redox balance favors formation of pyruvate as the 1995). Yoo et al. for specific acyl CoA molecules. and and interaction with lipid metabolism in algae. it may be of interest to research these TAG intermediates. naturally or under stress conditions. Since polysaccharides may still prove useful as intermediates diglycerides are usually present in high amounts in rapidly to other types of conversion processes and final fuels. though their physical structure and the presence in an acyl CoA-independent way (Arabolaza et al. degradation. 1971. via three sequential acyl transfers (from acyl CoA) These major storage polysaccharides represent potential in the endoplasmic reticulum (ER) (Exhibit 2. 12 2. as example many brown algae and diatoms. While these results provide cellulose and have other polymers that provide structure an indication that starch synthesis is linked to lipid to the cell (Raven et al. biochemical feedstocks for conversion to liquid fuels. it is important to Primary Pathway for TAG Synthesis determine the predominate types that are present.. an enzyme wall. accumulate well as of neutral storage lipids such as TAG.. The major pathway for the their main carbohydrate storage compound. these phosphatase releases diacylglycerol (DAG). featuring rigid such as triacyglycerols (TAG). mannitol. or fucoidin as 18 carbon fatty acids as precursors. It could. These structures can be an important source of that is unique to TAG biosynthesis (Lung and Weselake. and the Algal Carbohydrates technical challenges these structures may present in strain Algae are incredibly diverse in the kind of simple manipulation. for example for fermentation to produce alcohols. recently been demonstrated in bacteria. Diatoms are also unique among algae for the presence of silica in their cell walls. envelope similar to those of gram-negative bacteria. Other abundant polysaccharides. be fruitful to Most cyanobacteria have a peptidoglycan layer and cell further research de novo starch synthesis. some red algae and dinoflagellates.. Fatty acids produced in the chloroplast are sequentially 2000a and 2000b). and complex carbohydrates that they use for carbon storage and cell structure. The acyltransferases be broken down into simpler sugars before conversion involved in TAG synthesis may exhibit preferences into biofuels. TAG is formed by food reserves. Some red In microalgae. 1987). Dephosphorylation of PA catalyzed by a specific end product (Bird and Benson. therefore. 2002). Algal Biology  . and thus may play an for example. 2004).. Another important consideration in algal strains is the a third fatty acid is transferred to the vacant position 3 composition and structure of the polysaccharide cell of DAG by diacylglycerol acyltransferase. while some algae lack synthesis.1).. many membrane lipids and/or carbohydrates to TAG have algal cell walls from different groupings are cellulose. Vigeolas et al. must typically 2006. including de novo fatty acid synthesis in the stroma of plastids. As mentioned above. plants and yeast based.. such an interaction has been also algae also have a thick extracellular matrix composed indicated by studies on the diatom Cyclotella cryptica of important products such as agar or carrageenan. carbohydrates. In the final step of TAG synthesis. plant embryos or absence of other structural polysaccharides varies or seeds accumulated 40% less oil (Periappuram et al. Alternative pathways to convert from cells in mass culture. If carbohydrates Lipid Synthesis and Regulation are to be used as fuel precursors. resulting in the formation of the central considered less suitable for ethanol fermentation because metabolite phosphatidic acid (PA) (Ohlrogge and Browse. for The synthesis of cellular and organelle membranes. However. greatly. when trying to access DNA for genetic important role in determining the final acyl composition manipulations. 1992). are encased in a polysaccharide sheath (Hoiczyk and Hansel. cell walls entirely. 2008.starch synthesis was impaired or inhibited. growing cultures. been identified and partially characterized (Horn et al. the nature of the interaction is unknown. 2006). accumulate significant quantities of neutral storage lipids Many green microalgae are plant-like. An important lesson is the recognition of the diversity of algal polysaccharides and cell walls. 1988). Cyanobacteria often store large quantities incorporation of the fatty acid into a glycerol backbone of glycogen (Chao and Bowen. and extraction processes. are 3-phosphate. 2008). feedstock potential.

Thus. 2000. Because fatty acids are common precursors for the Alternative Pathways to Storage Lipids synthesis of both membrane lipids and TAG. Diacylglycerol (DAG) ER: Endoplasmic reticulum. Glc6P: Phospholipids glucose-6-phosphate. These pathways are worth investigating to use information regarding lipid biosynthesis in plants when developing strains for improved lipid production. This has revealed that algal lipid metabolism may be different lack of understanding may contribute to why the lipid from that in plants. Algal Biology  13 . PDH: pyruvate dehydrogenase (putative pathways were proposed in dashed lines). ACP: acyl carrier protein. involved in lipid metabolism in algae...1 Major pathways for fatty acid Phosphatidic acid and TAG synthesis in plants and algae 3-PGA: 3-phosphoglycerate. 2008.. Moreover. it will be difficult galactolipids. algal cell coordinates the distribution of the precursors to and the relative contribution of these individual pathways distinct destinations or how the inter-conversion between to overall TAG formation may depend on environmental the two types of lipids occurs needs to be elucidated. ER AGPPase: ADP glucose pyrophosphorylase. CO 2 Glc6P Starch AGPPase Central Starch Glc6P 3-PGA 3-PGA Amylases Metabolic Pyruvate Mitochondria PDH CO 2 Pyruvate Oxaloacetate Acetyl -CoA Chloroplast ACCase TCA cycle Acetyl-CoA KAS I KAS II Glycolipids SAD Galactolipase? C16 . If the or culture conditions. the potential pathway for lipid basal lipid and TAG content may represent an intrinsic formation from starch reserves mentioned earlier. 2004).C18 CoA Figure 2. Triacylglycerol Lipid Body Dahlqvist et al. enzymes and new scenarios for lipid production and strain improvement. Stahl et al. As an example. Accase: acetyl GDAT? CoA carboxylase. to develop hypotheses for strain improvement in algae. if any. the annotation of genes involved in lipid The regulation of the synthesis of fatty acids and metabolism in the green alga Chlamydomonas reinhardtii TAG in algae is relatively poorly understood.. Such pathways property of individual species or strains. 1998). GDAT: putative Acetyl- glycolipids: DAG acyltransferase. KAS: 3-ketoacyl-ACP. how the Algae may possess multiple pathways for TAG synthesis. 2005). Analyzing different algae could help ability to control the fate of fatty acids varies among algal to elucidate the possible pathways of TAG synthesis: the taxonomic groups or even between isolates or strains.. as indicated by the presence and/or yields obtained from algal mass culture efforts fall absence of certain pathways and by the size of the gene short of the high values (50 to 60%) observed in the families that relate to various activities (Riekhof et al. pathways. DAGT PDAT? PDAT: Phospholipids: DAG acyltransferase. and other 2. laboratory (Hu et al. If this proves to have not yet been studied in algae. Sheehan et al. it could be a challenge to extrapolate information DAG can also be used directly as substrates for synthesis learned about lipid biosynthesis and regulation in laboratory of polar lipids. de novo fatty acid and lipid synthesis should Understanding lipid regulation can help to maximize be studied in order to identify key genes. PA and be true. Similarly. the de-novo Kennedy Pathway. such as phosphatidylcholine (PC) and strains to production strains.

as an electron sink under photo-oxidative stress (discussed 2002). phatidylethanolamine or stress conditions as degradation of chloroplasts occurs. lutein. therefore. or astaxanthin) produced in cells under favorable growth conditions. Synthesis of Lipid bodies may dock with different regions of the ER TAG and deposition of TAG into cytosolic lipid bodies and plasma membrane. Algal chloroplasts the carotenoid pathway are sequestered into cytosolic contain monogalactosyldiacylglycerol as their main lipid lipid bodies. and thus relax the over-reduced electron related to storage lipid formation requires further study. stress conditions on lipid production in algae. cell division. 2002). an important aspect of membrane formation the role of oleosins (Murphy. some algal cells stop division and accumulate TAG as the main carbon storage compound. Such work is also important in algae to distribution and their great abundance in many lipid- better understand the interaction among organelles as storing seeds. including vitamins. and et al. the TAG synthesis pathway body accumulation occurs. thereby serving as a mechanism to been proposed that these alternative pathways that convert “detoxify” these compounds and deposit them in the starch. 1998. 1998). and spatial. the structure and function of lipid However. In oil-producing microorganisms. Because of the potential importance of TAG play an important role for cell survival under stress. a close relationship is often may also play a more active and diverse role in the stress found between neutral lipids like TAG and the membrane response. It has been suggested that oleosins may be primarily Oxidative Stress and Storage Lipids associated with the stabilization of storage lipid bodies during the severe hydrodynamic stresses of dehydration Under environmental stress conditions (such as nutrient and rehydration that occurs in many seeds (Murphy. the default pathway in some mitochondria and glyoxysomes/peroxisomes. Current work in plants is understandable in view of their exclusive localization is focused on deciphering lipid transport across plastid on lipid-body surfaces. the formation of fatty acids could help consume bodies. transport chain under high light or other stress conditions. Some acids. with acyl earlier). It has as acyl donors.. This and lipid fate (Benning.g. This relationship may be both metabolic.lipids (Alvarez and Steinbuchel. Carotenoid-rich lipid bodies serve as a (~50%). In addition to load or discharge their lipid cargo (Zehmer et al. their apparently widespread envelopes. The study the plastid where they are assimilated. and their interactions with other organelles excess electrons. and other components into form of TAG. excess electrons that and glycerol moieties exchanged between the different accumulate in the photosynthetic electron transport chain lipid classes.. Chloroplast membranes control the exchange of metabolites between the plastid and the cytoplasm. TAG synthesis may also phosphatidlyglycerol ( ~15%) (Harwood. 1992). which direct physical continuities between lipid bodies and may in turn cause inhibition of photosynthesis and damage bilayer membranes. the role of oleosins in the biogenesis of plant lipid bodies. membranes or they can be exported to the ER and hydrocarbons and very long-chain ω-3 and ω -6 fatty assembled into lipids for cellular membranes. as rapid lipid energy storage compound. starvation). and other macromolecules. 14 2. metabolism in algae. Algal Biology  . in order algae under stress conditions (Hu et al. sulfoquinovosyldiacylglycerol (~15%) and the chloroplast under stress. Lipid trafficking of lipid-body biogenesis in plants has focused largely on is. Nevertheless. with growing evidence of induce over-production of reactive oxygen species. 2008). Huang. Organelle Interactions and storage lipid formation warrants further study.. the chloroplast stroma is the primary location to Other Organelles for fatty acid biosynthesis in plants.potential pathways to convert membrane phospholipids and The TAG synthesis pathway is also often coordinated glycolipids into TAG. As mentioned Lipid Body Formation and Relationship earlier. excess membrane lipids. β-carotene.and glyco. there are now doubts about it relates to lipid formation and lipid trafficking. 2008). proteins. 2001).. The molecules their lipid composition dominates extracts obtained from (e. to the obvious physiological role of TAG as a carbon and 2009). with smaller amounts of digalactosyldiacylglycerol ‘sunscreen’ to prevent or reduce excess light from striking ( ~20%). 1993. The thylakoids of chloroplasts are with secondary carotenoid synthesis in algae (Rabbani the main intracellular membranes of eukaryotic algae. there have been relatively few studies on lipid glycerolipids assembled at the ER are then returned to bodies in algae compared with plants and fungi. In order to better understand lipid to membrane lipids. toxic fatty acids excluded from the membrane system the fate of these abundant lipids remains unclear. Zhekisheva et al. Fatty acids can then Despite the economic importance of algae as a source of be either assembled into glycerolipids at chloroplast a wide range of lipophilic products. Under utilize phosphatidylcholine. or with other organelles such as may be. The de novo TAG synthesis pathway can serve phospho. with exceptions. Under high light stress. the exact relationship between oxidative stress..

(b) competition for photosynthetic reductant at the level of ferredoxin. Gfeller the photosynthetic membrane for decreased efficiency of and Gibbs. and (d) inefficiencies in Biohydrogen the utilization of solar light energy (Seibert et al.. One of the most important characteristics also help provide information about photosynthetic of hydrogenases is that they are O2 sensitive. As a substrate. compete with hydrogenases for photosynthetic reductant. light. Either O2+2H+ water or starch can be the electron donor.2 P680 Two are driven by light and the third occurs in the dark. in the following reactions: 2H2O hydrogenases with improved tolerance to O2 (Cohen + light energy → O2 + 4H+ + 4e.0 PQ Cyt b CO2 Cyt f 0. (Drawing courtesy of Prof.2 Three different pathways for H2 production 1. metabolic Four biological challenges limiting biohydrogen production engineering through genetic manipulation represents in algae have been identified: (a) the O2 sensitivity of yet another strategy for the production of algal oils. 2000. those and carbohydrates. ferredoxin (FD) is the primary electron donor to the hydrogenase enzyme. or Sequence Tag (EST) analysis. Hydrogenases are the enzymes responsible for releasing molecular H2 (Ghirardi et al. efficiency of solar light utilization (Polle et al.Besides biochemical analysis to study algal lipids 2007). 2008). fermentative H2 pathway coupled to starch production (Mathews and Wang. and engineering their down-regulation during H2 independent. but the electron acceptor is switched at the level of ferredoxin (FD) from CO2 to protons under conditions that lead to H2 production. carbon partitioning and lipid/carbohydrate synthesis in algae. 2009). the light-driven pathways photosynthetic-electron-transport-coupled ATP production can either employ water (through photosystems II and (ATP is not required for H2 production). There are two major types of hydrogenases. (c) regulatory issues associated with the over production of ATP.4 Pheo QA QB 2H+ Photosystem II 0.2) (Melis et al. and a third. Three et al.2 P700* A0 A1 -0.. Posewitz. 2003). for example. a These challenges could be addressed by (a) engineering potential fuel product. studies involving Expressed containing iron (which are generally H2-evolving). (c) engineering degradation (see Exhibit 2. M.8 Fx H2 Photosystem I P680* FA/FB FD H2ase Energy (Volts) -0. (b) identifying metabolic pathways that pathways have been described in green algae: two light.4 PC Chl fixation P700 0. and (d) engineering I) or NADH from the glycolytic breakdown of stored the photosynthetic antenna pigment content for increased carbohydrate (through photosystem I). In all pathways.→ O2 + 2H2. Some microalgae and cyanobacteria can produce H2. and proteomic studies.. Colorado School of Mines ) 2. driven H2-photoproduction pathways. 2005). cDNA microarray both nickel and iron (which are generally H2-uptake analysis.8 Chl 2H2O Figure 2. Carbon is fixed under Photosynthesis normal photosynthesis with water as the donor. 1984).. hydrogenases. would enzymes). Glycolysis ADP ATP starch pyruvate Acetyl-CoA + CO2 NAD++H+ NADH PFOR NPQR NAD++H+ FD(ox) FD (red) -1. Algal Biology  15 . Based on such information..

2005). (b) remove or down-regulate competing fermentative pathways thus directing more of the cellular flux toward H2 Mutagenesis production. accounting for their ability to adapt quickly of hypotheses for strain improvements.. the importance of being able to harness and synthetic components) and synthetic photosynthetic biotechnology approaches to generate algae with desirable systems that mimic the fuel-producing processes of properties for the production of biofuels and bioproducts photosynthetic organisms. Thus. human health. Similarly economic viability. questions to consider in the context of mass production. Algal Biology  .. the limited algal to changing environmental conditions (Stal and Krumbein. formate. and 3) highlights a few via nitrogenase or hydrogenase (Stal and Moezelaar. to delete or disrupt genes.3 Algal Biotechnology produce H2 (Tamagnini et al. Using hydrogenase as the catalyst. From here. 2008). While a better understanding of the basic for H2 production at night. 16 2. several challenges generations and the possibility of unintended horizontal must be addressed: (a) improve photosynthetic efficiency gene transfer to other organisms are important research to increase the yield of carbohydrate accumulation. engineered than eukaryotic algae and have more O2. hexose) (Turner et al. methods to manipulate of photosynthesis. Methods to cultivate and process algae in photosynthesis has been demonstrated in the unicellular commercial settings are no less important to biotechnology. al. A possibility enabled new multi-billion dollar commercial enterprises to improve the efficiency of biological H2 production for agriculture. Prince and Kheshgi. 1987). for commonly used bacteria. Some of these methods can also be used to study the It is well established that dark fermentation suffers from localization of gene products (mRNAs and proteins) low H2 molar yield (less than 4 moles of H2 per mol within cells. cyanobacteria Cyanothece sp.. more knowledge cannot be overlooked. Genetic approaches are commonly also excretes typical fermentation byproducts including used to introduce genes. hydrogen evolution pathways. 1989). and CO2 (van der Oost et al. and (c) learn to express multiple hydrogenases The generation and characterization of mutants is a so that electrons from both ferredoxin (Fd) and NAD(P) powerful approach to understand gene function and H can serve as electron donors to support H2 production.. In order to fully realize the potential of H2 approaches. using the carbon reserves biology of algal growth and metabolite accumulation produced during the day. 1998). In all cases. However. Efforts should be undertaken to understand the fundamental To circumvent the inhibition of hydrogenase by O2. the unicellular non-N2-fixing cyanobacterium The Genetic Toolbox Gloeocapsa alpicola can evolve H2 from the fermentation Because biological productivity is a key driver for of stored glycogen (Serebryakova et al. examples of how algal biotechnology has been applied 1997). For algae that undergo sexual reproduction.. All cyanobacteria examined thus far employ the relevant strains remains a significant technical hurdle. fermentation is using modern analytical approaches will provide a wealth constitutive. using nitrogenase as the catalyst. fungi and higher plants. 2) describes were found to use pyruvate-ferredoxin oxidoreductase. the ability to improve on native strains under non-N2 fixing condition. they are typically more easily years of basic biology and genetics R&D. This is due to the production the toolbox allows traits to be recombined into a single of organic waste by-products described above along individual by mating parental strains. the stability of the desirable trait through many production via indirect biophotolysis. potentially generate strains with desirable characteristics. This temporal separation of H2 production from to date. enabling technologies and analyses that can be applied which reduces ferredoxin for subsequent H2 production for biofuels and bioproducts. 2009). 2008) and Oscillatoria (Stal and Krumbein. Collectively. genetic and cellular processes involved in the synthesis another option for H2 production is to take advantage and regulation of potential fuel precursors from diverse of the fermentation pathways that exist in some algae species of algae. genetic toolbox that can be used to modify process- 1987). While many of the challenges described above The biotechnology industry grew from more than 100 exist in these organisms. biological process engineering breakthroughs directly tolerant hydrogenases (Ghirardi et al. ATCC 51142 (Toepel et and these are the subjects of Chapters 3 and 4. several cyanobacteria tools available to modify algal strains. this subsection seeks to 1) address the genetic of glucose to pyruvate. and the production of includes developing biohybrid (those with biological chemicals. and to acetate. For all of these with ethanol. In cyanobacteria. Embden-Meyerhof-Parnas (EMP) pathway for degradation Thus.Recently there has been a focus on using cyanobacteria to 2. and algae genetically remain far behind those developed hydrogenase structure and function is required. the hydrogenase from is a potentially important element in the research effort Cyanothece PCC 7822 produces H2 in the dark and toward algal biofuels. 2002. modify genes or gene expression in a particular organism.

As long as an appropriate screening process is antibiotic use. diatoms (Cyclotella.. simplified identification of the mutated gene.. A direct comparison of the by introducing a selectable marker randomly into two has shown that the nourseothricin system generates the genome (Adams et al.. 1995). Nostoc. The mechanism of antibiotic native gene is to be entirely replaced). 1994). 1989. changes in fluorescence properties. Marker systems that take advantage of the ability to genetically complement Transformation Methods auxotrophic and metabolism mutants have also been Gene transfer systems have been established in many achieved (Kindle et al. whereas nourseothricin in gene expression. other approach is limited by the low frequency of naturally methods may need to be considered for production scale.. use of transposons (Miller and Kirk. Phaeodactylum. in the presence of an antibiotic).. Dunahay et al.. Dunahay et al. dinoflagellates (Amphidinium. which necessitates a large amount of screening. there marker (Hasnain et al. This the DNA is present. this resistance is a powerful tool for research. presumably because requirements for expression levels of the gene are lower and less taxing to the cells. Certain strategies can also enable changes antibiotic by the resistance protein... 1999). iterative be replaced in order to generate the phenotypic effect.g. Antibiotic in DNA replication can be identified. Arthrospira). large-scale algal production. 2. generated by conserved ribosomal protein mutations have e. Mutants are more readily generated by For research purposes. 1985.. constructs must be A powerful way to manipulate genomes is the ability to made to place the gene under control of an expression introduce DNA into the cell. Any mutagenesis approach requires an appropriate screening technique to enrich for and isolate mutants. or through the larger numbers of transformants (Poulsen et al.. 1993. Most of the current selectable markers are derived to exhibit a characteristic phenotypic change that is easily from bacterial genes. However. including resistance to neomycin. first concern is the cost of the antibiotic. Tagging can be accomplished is inactivated enzymatically. including cyanobacteria (Synechococcus. but markers based on resistance assayed. 1994). Dunaliella. are examples of control elements that work across along with the DNA of interest on an extra-chromosomal evolutionarily diverse species (Dunahay et al. and to select for cells in which element that functions in the species of interest. Several in mapping the locus responsible for the phenotype. occurring mutations. An algal strains. important consideration is that the use of antibiotics in Synechocystis. However. Debuchy et al. appropriate mutations must without the need to generate genetically engineered be generated in the species of interest. Typically.. Once an appropriate antibiotic resistance or Selectable Markers complementing gene is identified. large-scale production has two major drawbacks. to be expressed at a higher level than the native gene (Nelson et al. spontaneous mutants arising from errors with increased antibiotic-resistant microbes. reduced chlorophyll fluorescence (Polle et al. 1995).. For the latter.g. also been successful (Del Pozo et al. element called a plasmid. a modified copy of the gene that inserts elsewhere into zeocin resistance requires stoichiometric binding of the the genome. 2006). concern is the environmental implications of widespread Thalassiosira). Nelson et or increased neutral lipid accumulation via Nile Red al. Hallmann and Rappel. 2005). 1995). 1987) can be screening criteria.. For selection could be used to generate useful algal strains complementation approaches. Caveats are that the mutated gene may need staining (Cooksey et al. or that the native gene may need to Given a well-developed screening approach. selection marker to use includes whether the antibiotic Drawbacks of this approach include the introduction compound is sensitive to light and whether its potency is of multiple mutations in a genome and the difficulty modulated by the salinity of the growth medium. zeocin (Apt et al. the decision as to which antibiotic standard chemical or UV-based mutagenesis approaches.. Navicula. or markers. which could exacerbate current problems developed.. For example. The green algae (Chlamydomonas. 1985. 1999). and nourseothricin approaches rely on homologous recombination (if the (Poulsen et al. 2006). 2002). kanamycin (Hasnain Targeted or tagged mutagenesis offers the advantage of et al. however. Chlorella. 1989).. This Sophisticated metabolic engineering could require the can include either a requirement for mutants to grow under introduction of multiple selectable or complementary certain conditions (e. Targeted 1996.. The second Volvox). ideally in well- (GE) algae—something that could be desirable for characterized genes that can be easily complemented. or introduction of resistance can also be an important factor. Anabaena.. antibiotic markers have been developed for microalgae. this is accomplished by typically involves using control elements from a introducing an antibiotic resistance gene as a selectable highly expressed gene in that species. Algal Biology  17 .

Chlamydomonas reinhardtii. Algal Biology  . then chemically based methods of transformation useful in this regard in microalgae. 2005). Gene Expression Control Elements Porphyridium. which is useful for both nuclear and chloroplast slightly elevated. challenge to introducing DNA into a cell is the nature of allowing for precise control over the timing of gene the cell wall. (Deng and Capecchi. electroporation. Many transformation methods also exist with nitrate in the growth medium. RNAi operates “breed true” though a series of sexual crosses. only a small via homologous recombination is more desirable than percentage of selected transformants will have functional random integration because it can overcome phenotypic RNAi.. 1988. The selection process was based on a gene is present. Algal strains often contain expression. brown algae Gene expression control elements (also known as (Laminaria. Porphyra).Symbiodinium). traits... classical genetic approaches using from the ribosome (Valencia-Sanchez et al. One solution to this problem was developed in dominance issues when more than one copy of the C.. but considering the is the biolistic (“gene gun”) approach (Armaleo et al. Control element strength can be evaluated Other successful methods include electroporation by monitoring mRNA levels by quantitative PCR or (Shimogawara et al.. 1992). For most of these approaches.. Two general types of RNAi vectors can be constructed but this methodology could prove to be extremely – one containing an inverted repeat sequence from the important. The nitrate reductase promoter has proven cell wall. 2009). (Kindle et al. 1987). 1996). 1990).e. Identification of and biolistic approaches (Matsunaga and Takeyama. small sizes and used to target suppression of specific and traditional mutagenesis and breeding has been used genes by base pairing. If methods exist to remove or perforate the expression. use of single stranded DNA (Zorin et al. be desirable. 1991) or silicon carbide whiskers (Dunahay.. because it is induced could be applied. sexual crossing are not well developed in microalgae. 1995). Even on vectors containing both the genome (Dunahay et al.. 2005) and control translation by either 1950s (Bird and Benson. including conjugation. 1993)... Frequently. and another in which bidirectional only for a limited number of generations and must be transcription generates the double stranded RNA. selecting for functional RNAi can be problematic of microalgal cells generally integrates randomly into (Fuhrmann et al. Sexual Crossing Breeding of desired characteristics from a number of RNA Interference (RNAi) phenotypic variants can allow for strain development RNAi can be a useful tool to down-regulate gene without creating GE algae. red algae (Cyanidioschyzon. and repressed with for cyanobacteria. 1995). or reduced levels of expression may transformation (Boynton et al. and can be used to knockout genes. 2006). transgenes are overexpressed by A common method for introducing DNA into algal cells using strong control elements. 18 2. and so recessive genotypes or when dealing with redundant genes where traditional may not manifest unless that genotype is allowed to genetic manipulations are difficult. Many through double-stranded RNAs that are cut down to macroalgae species are capable of sexual reproduction. other inducible or repressible control elements would be useful for both research and commercial applications. 2004). RNAi can inhibit transcription to improve commercial varieties of seaweed since the (Storz et al. Gracilaria. ammonium (Poulsen and Kroger. crossed periodically to maintain culture viability. Undaria) and euglenoids (Euglena). RNAi approaches have been investigated primarily Homologous Recombination in the model green alga. a fundamental can be actuated by simple manipulations are desirable. used in a red alga (Ohnuma et al. transcriptional regulators) can modulate the levels Hallmann (2007) provides a comprehensive review of of mRNA. or co-introduction of recombinase genes with the transforming DNA (Reiss et al. With the exception of cleaving specific mRNAs or sequestering them away Chlamydomonas. Homologous recombination-based gene integration although there is a recent report of the method being approaches are common in many strains of cyanobacteria. inducible and repressible promoters that 1993). Gene replacement a selectable marker and an RNAi construct. DNA introduced into the nucleus sense. Dunahay. reinhardtii. 2005). especially in the study of polyploid organisms multiple copies of their genome. which can then subsequently affect algae algal transgenics and implications for biotechnology. high-throughput phenotypic screen for functional RNAi Successful recombination approaches have included the by co-targeting an amino acid synthesis pathway along addition of long flanking regions to the gene of interest with the desired gene of interest (Rohr et al. vortexing with glass beads high throughput transcriptomics (i. In a practical but less so in microalgae. 1998). 2001). intermediate. Some diatoms can be propagated vegetatively gene to be silenced. need for balance in cellular metabolism.. In addition.. microarrays).

Quality standards and appropriate data. Osteococcus tauris. Except for cyanobacteria. 2006). A genomic survey of representatives from all major algal classes is Approaches and Bioinformatics desirable. the macroalga Porphyra purpurea at the Joint Genome Institute characterization of these cellular processes will prove (U.Centre National de Séquençage (France). 2005. creating the new field of bioinformatics unicellular green algae (C.). Gaietta et al. Even though new sequencing technologies determining their intracellular location and can provide readily enable accumulation of data for large genomes. Bathycoccus sp. These biological of only a handful of microalgal species have been fully advancements have been complemented by developments in or partially sequenced to date. Together. and proteomics. Genome size in algae can vary substantially. Department of Energy) and for Ectocarpus siliculosus useful for applications. With the development of more powerful sequencing training should be established at the onset of activities methods. useful in monitoring intracellular metabolic processes associated with biofuel precursor production. pussilla. such as genomics. especially at the basic level of gene annotation. 2004). species within classes that make abundant fuel precursors. distribution of DNA between the plastid and nucleus (Wilhelm et al. It must be noted level. obtaining a genome sequence should Regarding core cellular metabolic processes. Algal species are being analyzed using these analytical and several diatoms (Phaeodactylum tricornutum. 2006). at least semi-quantitative evaluation of their abundance assembly of such data (especially with short read lengths) in a simple measurement. Micromonas in ways and timescales that were never before possible. Bioinformatics analysis of sequenced genomes. approaches to better understand the underlying Thalassiosira pseudonana. Indeed. including allosteric activation and metabolic though. cellular processes and regulation involved in Genome sequencing projects are also underway for the defining the attributes of the strain. a be strongly considered for any strains being developed substantial amount of regulation occurs at the protein for biofuels research or production.Directed Evolution of Enzymes/Proteins reduced and more coverage is obtained in a shorter period of time. forming the foundation for at Genoscope . transcriptomics.. Although time families). reinhardtii. visit http://genome.. Volvox carteri). Fragilariopsis cylindrus). 2008). for which over 20 completed these methods have revolutionized the study of organisms genome sequences are available. will be essential Sequencing and Annotating Algal Genomes to make sequence data usable. engineering so that they function more efficiently or have other favorable characteristics could be valuable for the development of algal biofuels technology. Algal Biology  19 . If not properly done. with a special focus on classes or individual High throughput approaches. applied research and technology development.jgi-psf.1 These species include computer sciences. Green Eukaryotic algae constitute members from at least eight fluorescent protein and its derivatives are the most major phyla.S. even in closely related species (Connolly et al. so it will be important to provide sufficient consuming.. but others have and secondary endosymbioses (Falkowski et al. that the genomic data are only as useful as feedback. 2002). This could 1 For a listing. a where powerful new databases and search algorithms are red alga (Cyanidioschizon merolae). Sequenced genomes are an essential basis of information bioinformatics can represent the largest stumbling block for the interpretation of transcriptomic and proteomic to achieving that goal. the nuclear genomes both in culture and in natural habitats. a coccolithophore (Emiliania huxleyi). all featuring a complex series of primary widely used and versatile protein tags. have enabled in-depth analyses to be performed in a whole cell context.. This information could be can be more challenging in repeat-laden genomes. in which costs have been substantially to ensure consistent and useful annotation.. a pelagophyte (Aureococcus annophageferrens).org/euk_cur1. approaches to modify proteins by genetic resources to allow for detailed analysis of the data.html 2. which could impact the regulation Enabling Technologies: “Omics” and processes of fuel precursor production. this level of regulation integrates the annotation (the assignment of gene functions or the proteome with the metabolome. One reason for this variation is likely to be the accumulation Protein Tagging Technologies of repeated sequences in the larger genomes (Hawkins Tagging proteins with fluorescent markers is useful in et al. several picoeukaryotes helping biologists share and build upon experimental results (Osteococcus lucimarinus. Undoubtedly. demonstrated utility and some possible advantages It is likely that the different symbioses have affected the (Regoes and Hehl.

Nannochloropsis sp. Dettmer et al.. as well as spectrometry. Cerutti more advanced than that of eukaryotic algae because and Casas-Mollano. Tanner et al... and function. These approaches not only enable protein atmospheric nitrogen and produce hydrogen. 2007. at least10 other algal genomes. identifying final fuel produced. transformation. and at least half a dozen selectable markers Proteomics are available for Synechocystis (Vermaas. Moreover. Algal Biology  . 2007) and should be applicable in cyanobacteria. however. but also allow for protein quantification and many can be genetically manipulated. which is the quantitative Therefore. approaches between related organisms and organisms maintenance. spontaneous considered in investigations of gene expression regulation. In the absence of genomic information. are underway. Lipid analysis is done using mass spectrometry of any algal biofuels technology development effort. and proteins. high-throughput sequencing technologies enable to Algal Bioenergy comprehensive coverage of transcripts and quantification of their relative abundance. response of living systems to pathophysiological such as Dunaliella sp. was sequenced over a decade ago. Comparative genomics weight compounds in a cell that are involved in growth. but they are prolific carbohydrate and secondary approaches allow for robust evaluation of soluble and metabolite producers. approaches (Han and Gross. Applications of Biotechnology New.. 1998). the first among photosynthetic organisms (Kaneko et al. Most transcriptomics approaches Cyanobacteria evaluate mRNA levels. there are still a large number of useful algal measurement of the dynamic multi-parametric metabolic species to sequence that are related to biofuels production. not related to lipid production or other biofuels efforts. proteomic Synechocystis has been used extensively for the study of approaches can also help assess the metabolic potential of carbon metabolism toward production of hydrocarbon organisms that may be difficult to isolate. Mass-spectrometry lipids. 2007). 1999).. Some strains can double membrane-associated proteins in the form of protein quickly (less than 10 hours). Transcriptomics Lipids are a subset of the molecular repertoire of the While genome sequencing will be an important component algae cell. Chlorococcum sp.. small RNAs also play Genetic manipulation of cyanobacteria is generally major regulatory roles in algae (Bartel. 2004..include the standardization of using a particular sequencing Metabolomics and Lipidomics approach that provides sufficient coverage of ESTs to The metabolome is the collection of small molecular ensure accurate gene modeling. in response to particular environmental conditions. but it is also emerging as a variety of different cultivation regimes can also help inform robust approach for genome-wide expression analysis process engineering and improve yields (Yu et al. double-homologous recombination. It is and nuclear magnetic resonance (Dunn et al. another dozen or so EST projects. and metabonomics. high Quantitative comparison of lipid type and abundance are throughput sequencing technologies will also become critical components of lipid-based biofuels approaches as increasingly important because it will not only help lipid characteristics can determine the suitability of the with genome annotation (for example. which survey.. Because the chemical that carry out similar functions can also help assign gene nature of metabolites varies more than for mRNA function and identify metabolic pathways of interest. notable that while these projects represent a very useful There is a distinction between metabolomics. making them detection of post-translational modifications (Domon and attractive organisms for biofuels production. or can determine fuels and intermediates. identification. Monitoring lipid characteristics under a coding regions of DNA). and ultimately determines how a cell functions Cyanobacteria generally do not accumulate storage in response to the environment. and Pseudochlorococcum sp. 2006). Many 20 2. 2009). 2006. and some strains can fix peptides. sp. Small RNAs have been many of the tools developed for bacterial genetics are identified in microalgae (Zhao et al. gas chromatography mass spectrometry. quantitative transcriptome profiling using new. 2005). Castellana et al.. and protein tagging are routine in some cyanobacterial systems. Scenedesmus stimuli or genetic modification (Nicholson et al. Both metabolomics and metabonomics may be important in terms of algal biofuels research. 2008). different metabolomic analysis tools are applied.. 1996).. the rationale for sequencing these organisms was involves the identification and analysis of metabolites. For example. especially with regard to translational regulation. including liquid chromatography mass Currently. Aebersold. The cellular complement of proteins reflects its metabolic potential. The genome of this strain functional diversity of a community of organisms.. 2005.

pathways and regulation of fuel precursor synthesis. is at least partly regulated by nuclear-encoded given their ability to accumulate high amounts of lipids gene products.. 2009) in Synechococcus. reported (Neupert et al. The genome sequence pathways can be manipulated for the benefit of biofuels of Chlorella NC64A was recently completed. 2009. In can accumulate under salt stress (Yoo et al. 2004).. strategies to address these issues are production pathway. Ferrante et al.. However. with specialized structure and metabolism that function some of which await elucidation and development. 2006. and chloroplast (PHB).2 Several production. salina is occurred during the evolution of microalgae.. 2002). Eisenhut et al. Synechocystis. Diatoms are responsible for 20% the interaction between the nucleus and chloroplast in of the total global carbon fixation (Armbrust et al. productivity under various environmental conditions. and isobutanol (Atsumi lipid producer.. proteome. 2007). reinhardtii can serve et al..photosynthesis and carbon metabolism mutants have been diversity of algae is important to identify species that generated. protothecoides.. 2007 ). the addition of an external carbon source Xiaoling Miao et al. it is known that glycogen accumulates under normal growth conditions in Chlorella is another well-studied genus of green algae..jgi-psf. It produces abundant Microalgae lipids (Weldy and Huesemann. whereas glucosylglycerol and sucrose and some species are abundant lipid producers. Synechococcus as a model system for understanding the fundamentals of and Anabaena strains have been studied for their hydrogen lipid synthesis and regulation. It is not an abundant ethanol (Deng and Coleman. It has also been shown that induces heterotrophic growth. and events likely had significant effects on the metabolic transgenic strains have been reported (Li et al. and metabolome (Singh et al. Continued exploration of the evolutionary A distinguishing feature of diatoms is their silica cell walls. but may also serve to guide the development of other types of algae for biofuels production. like the production potential (Tamagnini et al. 2007) and well developed transgenic secretion of cellulose.. it can be sexually crossed. 2008. fatty acid synthesis. 2007). 2008). Koksharova et al. Fulda et al. including glycogen. which occurs in the Diatoms were a major focus in the Aquatic Species Program chloroplast. and polyhydroxybutyrate Chloroplast transformants are also stable. anaerobically (important for the production of hydrogen). The genome sequence of D.. In order to redirect carbon to a fuel al. It has not been shown how these dry weight lipid (Xu et al.. which increases both growth PHB accumulates under nitrogen depleting conditions rate and lipid production. 2008). a comprehensive understanding due to codon bias (Heitzer et al.. RNA silencing of carbon metabolism and regulation is not yet available (Cerutti et al.. and stable and successful expression characterize the dominant carbon storage compounds of foreign genes in C. and because it has outstanding salt tolerance (from 0.. capabilities. C. and high-throughput analytical techniques are adept at making fuel precursors and with high have been applied to the study of its transcriptome. 1999). For example. 2006). 2008). which are cells is also often dependent on environmental conditions. Dunaliella salina has several useful characteristics for large-scale biofuels production. physiological conditions.. Relatedly. These studies can not only serve to advance examples of Chlorella transformation have been reported. Algal Biology  21 . sucrose (Nobles and Brown. and the conditions that trigger carbohydrate protein expression systems are well developed. reinhardtii is the fact that nuclear expression of foreign genes is still problematic Despite all of the progress. 2008). et al. accumulation. it can be grown under extreme conditions over 3 billion years of evolution. the understanding of how the production of different although the stability of the expression of the foreign carbon storage molecules are controlled in response to genes is still questionable (Leon and Fernandez. 2007). and positional effects (Ferrante et in all cyanobacteria.. Multiple endosymbiotic events organisms. 1998). resulting in greater than 50% (Miyake et al.. A possible drawback of C. algae with different extents of endosymbiosis (Wilhelm suggesting favorable growth rates for biomass production. The latter is a production of other carbohydrate-based storage compounds. For example. and there are fundamental differences in (Sheehan et al.. it will be necessary to further being developed.home. 2 http://genome. 2006). filamentous strain that can form heterocysts. 1997)... In addition to having a sequenced nuclear genome Transgenic approaches have enabled the production and (Merchant et al. 2000). Chlamydomonas reinhardtii is the most studied eukaryotic algae. 2003). reinhardtii has been recently been (sinks) in cyanobacteria. 2007.. and are highly diverse that could reduce the growth of possible contaminating (Falkowski et al.html 2. These currently being determined (estimated size 130 Mb). sucrose. 2004). glucosylglycerol.1 M to near Unicellular eukaryotic microalgae are the product of saturation). 2006. Lipid production. but nevertheless.

and choice of cultivation methods In general.doe. and holdfast for anchoring the entire structure to Despite the great promise of GE algae. Modifications They are typically comprised of a blade or lamina. species. Niwa et al. 2003). 2004. and expression profiling (Collen et al. and alternation of generations. Unlike microalgae. induction response. 1999.. Zaslavskaia (Jacobsen et al. 1996. 3 http://www. and regulatory gene expression control 2005). are well established for several diatom species (Dunahay et 1999. GE algae are defined as strains life histories. affinity with corresponding microalgal forms. which includes diatoms. Baldauf. They have low lipid content as a engineering of seaweed genes to create valuable products. Two diatom genome sequences are complete are used in seaweed population genetics (Alberto et al. Red macroalgae such as Porphyra spp. potential for large-scale blooms and subsequent is in a state of flux (Ali et al. Algal Biology  . anoxic zone formation.3 None of the sequencing projects has thus far et al. constructing detailed pathways evolutionarily diverse and abundant in the world’s oceans for secondary metabolite production. Moulin et al. 1997. 2001. However. Dutcher and Kapraun. taxonomic affinities are under re-examination 2008). Roeder et al. because silicon metabolism is not restriction fragment length polymorphisms (RFLPs) and believed to be tightly coupled with the metabolism of other random amplified polymorphic DNA (RAPD) analysis nutrients. each of the major macroalgal groups has in terms of organism containment. For the species displaying variations of annual and perennial purpose of this report. Advances in seaweed cell and molecular biology are Silicon limitation is one trigger for lipid accumulation currently being applied toward a better understanding in diatoms. Chlorophyta (green algae). 2005).and their requirement for silicon as a nutrient for growth. combinations of sexual and asexual carrying coding sequences obtained from a foreign reproductive strategies. and four more 1999.. the brown macroalgae such as the kelps are GE algae as production strains. 1995. Meneses and Santelices. et 22 2. being applied to understand cell function in representatives With the development of robust gene silencing approaches of red.. potential for toxin markers. 2003. Currently.. (Armbrust et al. general rule but are high in carbohydrates that can be converted to various fuels. brown. 2006) are elements have been identified (Poulsen and Kroger. there is nevertheless hard substrates in marine environments. Green macroalga such as Ulva (also known as sea lettuce) are classified together with common green microalgae such as Chlamydomonas and Chlorella as Chlorophyta. Niwa et al.. As such. such as the unicellular alga Porphyridium cruentum. Since the beginning of the deployment of GE organisms. represent a broad group of projects will facilitate efforts such as global genomic eukaryotic photosynthetic marine organisms. Ho are underway. For Despite the uncertainty regarding the development of example. Use of gene-specific probes al.. also have microalgal counterparts. Bowler et al. Understanding the basic biology that will inform with the use of molecular tools and phylogenetic such aspects as lateral gene transfer. 1995. 2008). and green seaweeds. the stipe. tools is still imperative from a research standpoint.. 2006. of GE organisms over the past 15+ years (GAO report. The life cycles a great deal of uncertainty regarding the need for or of macroalgae are complex and diverse. and strain selection and been focused explicitly on biofuels. there have usually been built in safeguards to prevent the release of GE organisms to avoid potential Macroalgae are historically classified as Phaeophyta disruption of ecosystems. development of genetic classified as Heterokonta within the Chromalveolata.. and Rhodophyta safeguards. 1999. 2000). there have been several unintended releases (red algae) on the basis of their predominant pigments. even with these (brown algae). Transgenic techniques characterization (Jin et al.. This is advantageous for studying the lipid of seaweed genetics and cell function.. 2005). with different the appropriateness of deploying these strains.jgi. the gene existing genes through selection and procedures such manipulation toolkit for diatoms will be fairly complete. Recombination of and possibly with homologous recombination.. 1994. 2005).. the status of macroalgal systematics production. they are Considerations of Genetic multicellular and possess plant-like structural features. and metabolic and coastal waters.. Genome sequencing Macroalgae. Fischer et al... are very important. Bouza et al. as protoplast fusion will be the basis for new strain creation where outplanting of individuals for growth Macroalgae in natural environments is a goal. For example. They are and proteomic profiling. Apt et al. or seaweeds..

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g. it is important to 3. Both types of cultivation systems must contend with require light to grow and create new biomass. Algal Cultivation  29 . photobioreactors). reducing the amount of water that Photoautotrophic Cultivation must be processed and thus the cost of harvest (Christi. However.1 compares the readily introduced. are unlikely to be sterilizable and may require periodic cleaning due to biofilm formation. Photobioreactors to identify the best-suited cultivation system. Photobioreactors can also provide a various features of both approaches). algae can be cultivated via maintenance is likely to be superior to that in open ponds photoautotrophic or heterotrophic methods. photobioreactors have suffered from and cyanobacteria. Algal Cultivation than for open ponds raceways.. but long-term culture Broadly speaking.. open gas exchange (both CO2 and O2). important that cultivation R&D they do not receive the benefit of evaporative cooling projects are closely associated with techno-economic and so temperature must be carefully maintained. industry.3. especially in terms of mixing and algal cultivation in closed (e. (TE) analysis that can evaluate the cultivation system Open ponds. capital costs maximizing light exposure. it is premature to predict whether problems of scalability. however. Microalgae and Cyanobacteria Given the multiple pathways to cultivating microalgae Traditionally. therefore. For photoautotrophic cultivation strategies where algae 2007).g.1 Cultivation Pathways acknowledge the advantages and disadvantages of both photoautotrophic cultivation approaches. both varying in where contamination and “foreign” algae are more their challenges and advantages (Exhibit 3. higher surface to volume ratio and so can support higher volumetric cell densities.1 Comparative features of microalgal cultivation approaches ADVANTAGES CHALLENGES • Less loss of water than open ponds • Scalability problems • Superior long-term culture • Require temperature maintenance as they Closed maintenance do not have evaporative cooling Photobioreactors • Higher surface to volume ratio can • May require periodic cleaning due to support higher volumetric cell biofilm formation Photoautotrophic densities • Need maximum light exposure Cultivation • Subject to daily and seasonal changes in • Evaporative cooling maintains temperature and humidity Open Ponds temperature • Inherently difficult to maintain • Lower capital costs monocultures • Need maximum light exposure • Easier to maintain optimal conditions for production and contamination prevention • Cost and availability of suitable Heterotrophic feedstocks such as lignocellulosic sugars • Opportunity to utilize inexpensive Cultivation • Competes for feedstocks with other lignocellulosic sugars for growth biofuel technologies • Achieves high biomass concentrations 3. Though photobioreactors (e. Many of these issues are being for closed photobioreactor construction are currently higher addressed through improved material usage and enhanced Exhibit 3. are subject to daily and seasonal in the context of upstream and downstream processing changes in temperature and humidity. It is. open ponds) or hybrid systems will prevail in the lose much less water than open ponds due to evaporation.

While it was determined that such cultivation presents a different set of advantages and structures would support growth of kelp. and culturalists to advance the vegetative propagation disease.. Hanisak. 2006) and field Additionally. Typically. 2005. environmental regulations from other sources. 3) ease of nutrient application without dilution. it remains to be seen which system protoplasts is possible in both the laboratory (Dipakkore will be preferred at scale over long periods of operation. they could compete for feedstocks and popular resistance against use of coastal regions for with other biofuel technologies. using artificially upwelled water produce a variety of products at large scale. 2004. appears especially open raceways. and major technological 1991). Dai et al. 2004. A related approach aquaculture represent challenges that will need to be is mixotrophic cultivation. seeds grown spp. such as sugars. This approach takes advantage of mature on growth structures in deep waters off the coast of industrial fermentation technology. Macroalgae 1987). 2006). Saccharina latissima and Nereocystis luetkeana have in greenhouses are attached to substrates (usually rope been successfully co-cultured with salmonid fish species structures). In sugars. which harnesses both the overcome due to the conflicting uses of coastal zones. important seaweeds such as Undaria (Kawashima and 30 3. 2005). availability of suitable feedstocks such as lignocellulosic which have viable seaweed aquaculture industries. (Chynoweth.. near-shore... Heterotrophic cultivation also achieves high successfully tested in the North Sea (Buck and Buchholz. Porphyra been in practice since the 1950s. 1993. Laminaria hyperborean. Buck. Macroalgae can be cultivated in off-shore. Sargassum. 2008). and predation. thus providing optimism for future the infrastructure required to grow the algae (Xu.. linked turbidostats or chemostats (Benson et al. considerable scale-up from current activities. Kumar et al. and 5) possibility of farm operations of seaweeds through cell and tissue culture techniques. then reared to plantlet size and transplanted (Bruton et al. to generate Biomass Program through several deployments of kelp new biomass. efforts. been adopted by seaweed biologists (Garcia-Reina et al. providing high cell density algal inocula promising for growing plants from protoplasts (Dai et for production ponds (Ben-Amotz. 2005). and presented (see Chapter 10). or Heterotrophic Cultivation in open pond facilities. already widely used to Southern California.. 2008. over those in water have been listed as 1) ease of plant management.. In the latter. Demonstrations of successful callus formation and improvements in efficiency of water movements and plantlet regeneration have been reported in commercially pond construction costs are needed (Friedlander. algae are grown without light seaweed farms was initially tested by the Marine and are fed a carbon source. 2007). located in close proximity to conversion operations Although the field is still at an early stage of development. wastes from the other species of artificially produced seed as a source of propagules has represent a nutrient supply for the macroalgae. photoautotrophic and heterotrophic ability of algae. 1993) and the phycocolloid-producing seaweeds pond and photobioreactor systems have been published or Gracilaria. Hypnea. For contribution to a biofuels the micropropagation of plants is a concept that has marketplace. encountered with the stability of either the structures Optimal conditions for production and contamination themselves or the stability of the attachment of kelp to prevention are often easier to maintain. Because these systems rely on primary productivity the United States and Europe. 2) use of plants with or without holdfast Modern tools developed in the larger plant breeding structures.. 1995) or a series of al.. 2007). As a result. improvement in strain selection. modern prototypes for offshore potential to utilize inexpensive lignocellulosic sugars for growth of the kelp. 2004. biomass concentrations that reduces the extent and cost of 2004. 1993). Algal Cultivation  . have been algal growth. Turbinaria. Near-shore coastal environments are already The primary challenges with this approach are the cost and being exploited by countries like China. difficulties were challenges compared with photoautotrophic methods. and al. and there is the the structures. 2004. 2009). Modern integrated aquaculture scenario in co-culture with finfish macroalgal cultivation technology that is based on the use and mollusks. Advantages of the land-based systems to coastal farms for grow-out to harvestable size. in hybrid systems. for these analyses is based on assumptions or proprietary Kumar et al. Land-based pond systems have also been considered for macroalgal cultivation (Friedlander.. Though TE analyses for both open Tokuda. 2002).. community are now available to seaweed biologists 4) avoidance of open sea problems such as bad weather. The operation of large offshore In heterotrophic cultivation.. Dipakkore et al. Dai et al. et al. Growth of plantlets regenerated from data. Japan. in particular. However.. much of the information used Gelidiella (Collantes et designs. photobioreactors could (Dai et al. both as free-standing algal farms and in an Macroalgae require unique cultivation strategies. Recent studies have play a critical role as breeder/feeder systems linked to shown that Porphyra. Heterotrophic as a nutrient source. Reddy et al.

. Some members of the a mixed or natural assemblage of organisms in an attempt community will be of positive value. little is known about might limit such takeovers? artificial pond ecology or pathology. limited.. 2006 ). 2009). predators. an investment in “open source” test bed facilities for public sector Important questions concerning this threat RD&D may foster more cultivation research. to large-scale algal cultures include: Nutrient sources and water treatment/recycling are • Are agricultural or municipal waste streams—a technically trivial and inexpensive at small scales and potentially significant source of nutrients for algal yet represent major technical and economic problems at cultivation—actually a liability because of significant commercial scales.. Nutrients. such as those that to maximize total harvested biomass.g. and lipids still others will cause culture disruption. monocultures are inherently difficult to maintain combined. Because of the and even less is known about host resistance mechanisms. and investigation into these areas will be important for the development of large- scale cultivation risk mitigation and remediation strategies. 2005. as well as potentially provide • Culture stability. e. Tapping into existing agricultural or reservoirs of algal pathogens and predators? municipal waste streams will lower nutrient costs but could • To what extent will local “weedy” algae invade introduce unacceptable pathogens. Wilson et al. 2008) productivity analysis. 1994. Others will compete for shared resources. and the use of algal mat or biofilm cultivation must be developed on scales that are orders of magnitude schemes. The methods must be sensitive. 2008. 2008. automated or semi-automated biological and chemical monitoring in production settings will Four broad cultivation challenges have emerged be essential for assessing the health and compositional that are important to address for economically dynamics of algal cultures. TSA- • Nutrient source scaling. although current has yet to consider the intricacies of biological carbon understanding of their diversity and host range is very concentration mechanisms (Wang and Spalding.. sustainability and FISH (Marie et al. Chytrid fungi have also been known to cause The inherent difficulties of scaling up from laboratory the collapse of industrial algal cultivation ponds (Hoffman to commercial operations present both technical et al.Other options for algal cultivation are being investigated Stability of Large-Scale Cultures including the harvesting of naturally occurring marine algal Systems for large-scale production of biofuels from algae blooms.. proteins. 1999. it will be and require significant investment in methods for detection challenging to maintain algal monocultures on this scale.. competitors.. Methods for rapid. chemical compounds. including worrisome components of large-scale algae cultivation CO2. 2006. “Environmental” DNA sequence analysis can contribute to the development of PCR-based • Standardized metrics for system-level (Zhu et al. 2009). One of the more into a variety of useful products. pervasiveness of issues related to scale. and pathogens. 2005). Fungal of supplemental CO2 in large-scale algal cultivation and viral pathogens are common. Continuous monitoring will management... • What prevention or treatment measures Wilson et al. especially in open larger than all current worldwide algal culturing facilities systems. Viprey et al.. or flow-cytometry-based taxonomic assays. and recycling.2 Scale-Up Challenges species. The cost-benefit analysis al. and pathogens is expected (Hoffman et al. and simple and complex carbohydrates. for real-time monitoring. In certain cultivation systems. or and take over bioreactors and open ponds? heavy metals into the biomass stream (Hoffman et al. 2008). Additionally. Wilson et al. Rittmann et al.000 and several million phytoplankton 3. must also be managed in a way that balances is the fact that algal predators and pathogens are both productivity and pathogen sensitivity with the plasticity of pervasive and little understood (Becker. 2004. management. there have only been 150 formal descriptions of phycoviruses. and management of competitors. Cheng et al. commercial-scale algal cultivation: selective. and inexpensive. Algal Cultivation  31 . 2008. 2004).. predators. viable.. Brussaard. it may become necessary to understand and manage the One possible approach to contend with this is to cultivate communities that will be present. It should be noted that. 3. (2009) point out that though there may be between 40.. but very little is known about host specificity and economic barriers to success. This model would can scavenge and recycle nutrients or synthesize essential require a downstream biorefinery capable of processing vitamins. Boutte et al. Honda et algal physiological adaptation. and be necessary in open systems since seasonal variation in • Water conservation..

developing an understanding of pond transportation and the volatile market for carbon credits will speciation.. Algal Cultivation  . invasive species.Furthermore. 2008. The frequency of contamination screening in algae have been developed and applied to events that require decontamination/restarts will be an many different types of phytoplankton with mixed results important parameter in the cost of production because of (Cooksey et al.. The cost of CO2 based nutrients for heterotrophic growth will also affect the economics and sustainability of such systems. Research at the interface between basic algal biology and chemical. trace metals. out (Abelson. 32 3. predator-prey relationships. automated. Along with standards. fluorescent indicators other than Nile Red necessary. and iron additions represent a long been known to increase algal growth rate. as siting requirements for efficient considered.S. result in increases in lipid content (Azachi et al. This calculation takes into account a 50% between using algae to scrub CO2 from emission gasses rate of nutrient recycle. accounting for 6-8 cents per this area is receiving new attention from the search for gallon of algal fuel in 1987 U. densities. metabolites. Reed et al. 2008). molecular-level algal ecology will be an important component of the investment There is an immediate need to standardize productivity portfolio required for developing the potential of algae.. nitrogen nutrition has long been known to affect lipid accumulation in phytoplankton Nutrient Sources. research in multi-trophic. and pathogens will be a key to the success of remedial actions and for determining Better methods to detect the amount of desired fuel when decontamination and subsequent restart procedures precursor produced will be required to assess the represent the only alternative. sustainable fuels. dollars (Benemann and renewable. Benemann and Oswald. but vary depending upon the specific strain and algal cultivation may rarely coincide with high-volume water source chosen. supplemental CO2 has Nitrogen. light stress in some marine phytoplankton may also The primary focus is the major nutrients – nitrogen. fit-for-purpose fuels. predators. 1999). civil and mechanical engineers is needed for cultivation science and engineering will yield significant rapid progress. and silicon (in the case of diatoms). and products. 1999. 1996. derivatives of the Bodipy for both wild-type and genetically modified algae. productivity lost during down time. For example. as well as others such potential need to either discard or treat the contaminated as Near Infra Red spectroscopy. and genetic.. 2009) and a focus especially important issue as there have been calculations on better understanding the mechanisms of biological that the world’s supply of phosphate is in danger of running CO2 concentration from ambient air (Lapointe et al. phosphorous. Eltgroth et al. coordinated research amongst analytical System Productivity chemists. rates. More recent data suggest that high salt and high impact on cost. and production siting. 1987. Nutrient supplies for algal cultivation have a sizeable 1998). New approaches are split Oswald. These tools. Spalding 2008). and normalization. molecule with higher lipophilicity or lower quantum yields whose competitiveness in the field cannot be accurately in aqueous solvent may prove to be more reliable indicators predicted. and Management 1981. need to be more rigorously culture prior to water recycle. diverging dynamics will be important. Gao et al. cultivation schemes should lead to enhancements in productivity. Thus. must also be R&D in both areas. 1938.. a significant investment toward basic of algal lipid contents (Gocze and Freeman. Utilization of existing and in the development of an algal biofuel industry could new knowledge related to the physiological regulation of facilitate the deployment of algal based-biofuels by lipid or carbohydrate accumulation coupled with scalable ensuring consistent. phosphorous. The development of studied. Phosphorous appears to be an (Rosenberg et al. There is justification to carry out such as sulfur. and significant operating cost. The synthesis of new non- genetic modifications of production strains may become toxic. permeable. Sheehan et al.. iron. inexpensive chemical treatments or physiological adaptations and high-throughput monitoring. From a productivity standpoint. National and international efforts toward improvements in productivity while at the same time generating quality assurance policy standards early on lower the cost of production. Fluorescent and Nuclear also inform efforts at developing robust. (Ketchum and Redfield. Early detection schemes for business models are already apparent on these issues. 1994). Shifrin and Chisholm Sustainability. competitive Magnetic Resonance-based methods for rapid lipid content production strains. vitamins. Douskova et al. sustainability. 2002). biochemists. and because of the 2005. The use and availability of carbon- point sources of CO2 (see Chapter 9). etc. and adapted for rapid. Dynamic pond monitoring will be important are also important. Requirements for additional nutrients. This information will productivity of potential strains. models and establish protocols for measurement of yields.. Strain selection should take nutrient requirements into account. physiologists. 1996). For example. 2008.. and ecology be a major challenge for TE feasibility studies..

the culture medium pose a problem for waste water energy. or silicon) as a economics. a certain amount • Geographic Information System (GIS) analyses of of “blowdown” water must be removed to prevent salt wastewater resources to understand availability. or industrial unsuitable for land crops. gallons per day from the 1 ha pond. phosphorous. Water Management. From a to fossil fuel prices.g. unused nutrients in • TE and life cycle analysis to understand the cost. biogas. from anaerobic digestion sludges can feed subsequent algal growth).. and it is not practical to transport wastewater In desert areas. nitrogen. The final fuel product from algal oil biology. however. 1989). for example. and the very large required energy sustainability perspective. 1998). also become necessary to expand the limits of analysis to include recycling of nutrients from animal waste. means to induce oil accumulation in the cells (Sheehan Important research areas therefore include: et al. evaporative losses can exceed 0. these nutrients utilize fossil fuels (particularly natural gas). At the same time. which is provide some of the nitrogen and sulfur needed from a component of the diatom cell walls. Douskova et al. For example. to be more valuable than using the spent biomass for It is possible to consider the use of nitrogen-fixing products such as animal feed. produced 3.. A potential problem with this approach however is the impact on facility siting. real economic or sustainability problems or because of Utilizing agricultural run-off also poses economic benefits loss of public support due to perceived problems. and sustainability implications of various discharge. for example. disposal will become a problem impact of such sources on algal biofuels production due to concerns of eutrophication of surface waters. Careful control of nutrient levels is also critical.. The processes through be diverted from carbon fixation to nitrogen fixation. by preventing eutrophication. The ideal form of nitrogen Another approach to reduce nutrient costs is to pursue is a function of relative costs and the specific strain’s a diligent recycle. Agricultural- or commodity-grade nutrients are more applicable. Finding inexpensive sources of nutrients will be important. If the biomass residues are. On the other hand. With large cultivation systems. too much of a particular nutrient may prove toxic. buildup. Therefore. Although economics dictate that the bulk of nutrient sources and recycling scenarios water derived from the harvesting step must be returned • Studies to explore the mechanisms of nutrient to the cultivation system (where remaining nutrients recycling. but One of the main advantages of using algae for their costs are still significant.5 cm per over long distances. 1996). In the future. which these nutrients are re-mobilized and made available which may or may not be compensated for by the “free” for algal growth are not well understood. productivity. aquifers and seawater.000 and compatibility of wastewater resources is warranted. which is a loss of 13. 20 metropolitan areas with high land prices and limited land cm deep open pond will require 530. nutrient recycle may prove inputs should be accounted for in life cycle analyses. water breakdown of organic materials and to sequester nitrogen can easily become a “show-stopper.. This may be nitrogen. treated by anaerobic digestion to produce perhaps in co-culture with eukaryotic algae. costs are tied end up primarily in the spent algal biomass. tend to be near enormous. brackish. Note also that flue gas fed to algal cultures may particularly problematic for recycling of silicon. Limitation of a key nutrient will have serious impacts on biomass Nutrient sourcing and the control of nutrient productivity. If this blowdown water contains substantial compatibility with cultivation sites. 2009). However. Currently. Though the water used to initially fill the pond can be saline. Because synthetic nitrogen fixation processes contains no nitrogen. Also. utilizing the biofuels production is their ability to thrive in water nutrient content of municipal. If not addressed adequately.” either because of and phosphorous into biomass for water clean-up. Further research into the availability day (Weissman and Tillet. a hypothetical 1 hectare (ha). agricultural. Reagent grade sources of nutrients could make the price Conservation. or iron. e. and sustainability issues.Nitrogen is typically supplied in one of three forms: ammonia. Algal Cultivation  33 . or urea. algae are used in some wastewater treatment facilities water management poses some of the largest issues because of their ability to provide oxygen for the bacterial for algal biofuels. but it may also be desirable to use nutrient levels are vitally important factors for cultivation limitation (e. phosphorous.g. then most of the nutrients will remain in the such a scheme will certainly have some impact on overall digestor sludge and can be returned to the growth system productivity levels as photosynthetic energy could (Benemann and Oswald. water demands will be Wastewater treatment facilities. and Recycling of a gallon of algal fuel cost-prohibitive.000 gallons to fill. and potential nitrogen and phosphorous. nitrate. 2005. availability. such as saline water from waste streams is a very attractive alternative. cyanobacteria as a way to provide nitrogen biologically. it may NOx and SOx (Doucha.

moving around such large volumes of water is very energy-intensive and can impose a significant cost. and treatment (e. accumulated salts. which could contain salts. or may require decontamination. This is an enormous amount of water (260. however. the water being lost to evaporation practiced by the oil industry. by the Environmental Protection Agency and already quality water stream. it is desirable to recycle most of that water back to the culture. The blowdown water exiting the process will also most likely require treatment.1999). process. residual nitrogen and phosphorous fertilizer. Note however • Analysis and definition of the regulatory landscape that evaporation plays a critical role in temperature surrounding discharge of water containing various maintenance under hot conditions through evaporative levels of salt. but live cells could adversely is fresh water. materials in the culture. from flue gas).” The amount of blowdown required for Because of the importance of issues surrounding the use salinity control is dependent upon the acceptable salt level of water.. Incoming water (surface water. makeup water/recycle. and water double their biomass on a daily basis. • GIS analysis of water resources. by disposing of a portion of the pond volume each day as “blowdown. toxins. flocculants. and residual live algal cells. heavy metals (e. Algal Cultivation  .000 gallons per day in the 1 ha example above). meaning that half movement (pumping costs) the culture volume must be processed daily. • Investigating ways to reduce the cost of water and location. Surface disposal and reinjection into wells may be an option as regulated 34 3. To contain costs.g. Treatment may be essential for water entering and exiting the process.) of the recycled stream could be cost-prohibitive.. toxins (including heavy cooling. or biological inhibitors produced by the strains themselves could impair growth if recycled to the culture. water. or other remediation before use. A critical water consumption. This could include reducing part of the analysis that goes into siting an algal facility evaporative cooling loads through such strategies will be to analyze the “pan evaporation” rates at specific as selecting thermotolerant strains of algae sites in conjunction with water cost and availability. An advantage of sites that may have lower pan evaporation rates closed photobioreactors over open ponds is a reduced • Understanding the long-term effects of drawing rate of evaporation.water from oil wells. However. municipal wastewater. or seawater) may be suitable as is. Some actively growing algal cultures can treatment. could pose a serious problem. wastewater. and other in the dissemination of genetically modified organisms. This can be prevented by adding Sterilization of blowdown water. and live cells or employ cooling towers to keep cultures cool will lose some if not possibly all of the water savings of such • Developing cultivation systems with minimal systems under these conditions (Flickinger. would be a fresh water—a costly and often unsustainable option—or very costly and energy-intensive proposition. and their proximity to utilizable cultivation through facility design and siting. disinfection. activated charcoal filtration. or other low. desalination. etc. while effectively Water recycling is essential. accumulated toxics. The added cost of such systems must down saline aquifers. chemical flocculants used in harvesting. flocculants. including the geology of these be balanced against the cost savings and sustainability aquifers and associations with freshwater systems analysis for water usage for a given location. Disposal of the spent water. but the amount that can managing the accumulation of salt and other inhibitors be recycled depends on the algal strain. including saline Conservation of water can be addressed to some extent aquifers. • Studying water recycle and methods to maximize recycle (and minimize blowdown). Furthermore. and continually making up the volume with affect biodiversity of neighboring ecosystems or result low-quality water will concentrate salts. Closed systems that spray water on the surfaces metals). research in the following areas is warranted: in the culture and the salinity of the replacement water.g. groundwater.

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and recycling issues. energy costs climb steeply. however. at least 1% algae given the known processing strategies (Borowitzka. Flocculation is used to increase the additives that have been studied. Culture manipulation techniques. Poleman and Pauw. 1988. water form of forced flocculation usually involving chemical recycling and flocculate recovery are required. electroflocculation and electrocoagulation offer with as low as 0. Pan et al. 1993. lime. well-managed high growth rate cultures due to their • Bioflocculation. Botes and Vanvuuren. the presence of flocculant in 4. water quality. Chemical additives that bind algae or Flocculation and Dissolved Air Flotation (DAF) was otherwise affect the physiochemical interaction between established for sewage treatment and later studied in algae are known to promote flocculation (Lee et al. 2004. and small size (~1 to 30 µm). 2006.. additives. consider the imagine selecting/designing strains to aggregate on cue energy costs and siting issues associated with harvesting or designed with a particular flocculant interaction in and dewatering. 1988). Knuckey et al. Bare et al. therefore.. circulation. electroflocculation. algae harvesting (Sim et al. and off-shore pH with and without additives is also effective.. in recycled water on culture health and stability and lipid production must be understood and controlled. combined with RD&D to be useful for promoting flocculation.. the mechanism slurry concentration also impacts plant location because of algae-flocculant interactions. Bioflocculation where algae are co- dewatering. and then air is bubbled 4. impact the required energy input. 1971). is required to promote sedimentation at harvest. mixtures. Future research in develop specific processing technologies to support those flocculation chemistry must take into account the following: designs and a fundamental understanding of how algal biology can impact harvesting and dewatering strategies. Koopman and chitosan. 1991. Manipulating suspension size of the algae aggregates. being considered. 1998. Flocculation and Dissolved Air Flotation A number of different forms of forced flocculation have been employed.. type. A determinations in particular processes. 2003. and chemistry to maximize algae recovery of dry biomass increases. only now being recognized as critically important. and on empirical of transportation. Kwak et al. McGarry and Tongkasa.. Cultures Finally. surfactants. and Shelaf. Processes that pertain to unicellular algae are quite different from the approaches applicable • The effect of residual flocculant or pH manipulation to macroalgae. Downstream Processing: Harvesting and Dewatering Conversion of algae in ponds.02 .4.1 Approaches for Microalgae further downstream extraction and fuel conversion processes must be understood and controlled. 1983). This facilitates the transport of electrocoagulation must be scaled-up with cost and cells to the photoactive zone through pond or bioreactor energy analysis. bioreactors. Alum. 1975. and other man-made fibers are some chemical Lincoln. Likewise. Flocculation leading to sedimentation occurs • Optimized sedimentation tank designs with integration naturally in many older cultures. and fuel Microalgae and cyanobacteria remain in suspension in conversion and use must be considered. 2005. lipids cultured with another organism that promotes sedimentation and carbohydrates).07% algae (~ 1 gm algae/5000 the advantages of no added chemicals (Chen. 2001). It is possible to feasible algae-to-fuel strategy must. salts. Phochinda and White. CO2 depletion for pH control has been studied (Sukenik Butterfi and Jones. Final will very likely depend on strain selection.0. make harvesting more difficult. The final slurry concentration will depend on the extraction methods employed and will Optimizing flocculation methods. some into further downstream dewatering techniques. cellulose. and systems to liquid transportation fuels requires processing autoflocculation in the form of photosynthetically driven steps such as harvesting (Dodd and Anderson.. 2004. therefore. These energy-intensive processes are has also been considered (Lavoie and Delanoue. In managed cultures. Edzwald. 1969. Downstream Processing: Harvesting and Dewatering  37 . may analysis of engineering designs. 1987). Addressing these issues requires careful mind. 1984). and extraction of fuel precursors (e. 1997).. gm water) must be concentrated to slurries containing Mollah et al.g. • Chemical flocculant recovery techniques are required Processing technologies depend on the algal feedstocks to minimize cost and control water effluent purity. polyacrylamide polymers. 1977. As the desired percentage concentrations. Their small sizes. Harvesting • The environmental impact of flocculant or pH Flocculation and Sedimentation manipulation in released water effluent.

• An important step is recovering the algal biomass from which can involve mowing with rotating blades. The flocculant efficiency. methane drum size. 2009). however. the growth substrate can be easily removed. 1996). energy savings must be realized before any widespread implementation of this approach can be carried out. For instance. have also been developed. leads to blinding. and mechanized harvesting methods. but doing or dredging with cutters. 2003). Filtration Solid/liquid filtration technologies are well studied. about 90% is (Oswald. Durability and 4. Air-drying is possible size will affect filtration efficiency and blinding rates. In addition to processing of large quantities of water and algae. or ships for operation. design. the filter. These include. Application of mechanical 38 4. such mechanized harvesters require boats should consider minimal or no washing requirements. and high throughput encountered in flocculation and DAF. in low-humidity climates.2 Approaches for Macroalgae blinding are also issues. Solutions involving either solar or wind efficiency. and can be optimized through are harvested from higher organisms (e. Other Harvesting Techniques and filtration without prior flocculation can be used A number of other techniques at various stages of to harvest and dewater algae (Ferguson et al. Saidam and Butler.g. and flocculant reclamation) are also investments. and have the ability to remove the algae later.through the suspension causing the algal clusters Centrifugation to float to the surface. suction. Filtration designs Invariably. Decreasing pore Because drying generally requires heat. shrimp and further understanding of several issues: tilapia) grown with algae (Johnson and Wen. but will require extra space and • Filter material also influences filtration and recovery considerable time. Downstream Processing: Harvesting and Dewatering  . The algae-rich top layer is Centrifugation is widely used in industrial suspension scraped off to a slurry tank for further processing. acoustic focusing to concentrate algae at nodes. operating costs. 2008). manipulation of Filtration is conceptually simple but potentially electric fields. recovery is largely dependent on current level of centrifugation technology makes this bubble size and distribution through the suspension.. water and technologies must consider large initial capital equipment algae purity. • The filter pore size is critically important as it is defined by the size of the algae species and algae Drying aggregation rate. approach cost-prohibitive for most of the envisioned DAF facilities require optimized integration with any large-scale algae biorefineries. Culture purity However. of the roughly 1. Modern seaweed harvesting vessels can be equipped with pumps to direct harvested seaweeds directly into nets or other containment structures (Ugarte and Sharp. Significant cost and engineered design for further downstream processing. 1995. the costs climb steeply with incremental becomes important as a distribution of microorganism temperature and/or time increases. Moving filters Currently. floc optimization. 2002.g. Centrifugation for sedimentation (e. the Microalgae and cyanobacteria present unique filtration use of organisms growing on immobilized substrates challenges because most strains considered for energy where the amount of initial water is controlled and feedstocks have cell diameters less than 10 µm. • Filtration design is an important variable with both Harvesting static and dynamic filtering operations. pores. pores decreasing filter efficiency. where fuel precursors very expensive.. 1991).. filter materials with controlled hydrophobicity and/or algae affinity can be developed. R&D have been proposed to harvest and dewater Downing et al. Manual harvesting is common for both cultivated and natural systems. Materials can be used that optimize filtration energy are also possible. Small algae pass through larger Drying is required to achieve high biomass concentrations.. but are not limited to. and reduction of filtering rates. The efficiency is dependent on All of the issues arising from the use of flocculants the selected species (as related to size). separations and has been investigated in algal harvesting (Molina et al. and bioharvesting. so leads to re-dilution of the product. 2001). Washing the filter is one practice.. microalgae. Power costs will certainly influence derived from cultivated sources (Roesijadi et al. the blocking of filter dryers and other oven-type dryers have been used.6 million dry metric tons have been used in drum and cylinder press designs of total seaweed harvested worldwide..

Algae that are grown in suspension The cost of harvesting and dewatering will depend on the culture. and hydrothermal liquefaction have either as Macrocystis. adhering epifauna and other forms of Ultimately. may be amenable to mowing. be harvested and the intervals between harvests to allow growth and recovery of biomass (Ugarte and Sharp.. could be cultivated in may be more important as a method to increase shelf-life floating pens. 2004). stones. Further. Although have been put in place in some countries (Pringle and some dewatering of seaweeds whose water content Tseng. 2009). litter. will have higher reaction efficiency during anaerobic digestion for biogas. a unit operations analysis of energy input debris that should be removed before further processing. To manage seaweed harvests. and low growing attached forms such as and reduce weight and associated transportation costs if Gracilaria will require different approaches compatible with algae are to be transported from sites of harvest to distant their growth characteristics. e. 2008).. Anaerobic digestion. by washing A critical gap is the energy requirements of these processes • Milling are not only largely unknown but unbounded. with needs is required with consideration of capital equipment the degree of screening dependent on the mode of investments. In forms such as Laminaria processing plants (Bruton et al. The exact nature of such mechanization will obviously depend on the form Macroalgae have less of a demand for dewatering as of cultivation and type of algae being cultured. new strategies should be developed to combine and integrate Preprocessing these processes in order to take an algae culture and The general preprocessing requirements for macroalgal convert it into slurry of a specific concentration. influence. This has important implications for plant design to answer simple • Dewatering questions like “What percentage of the total plant energy requirements or what percentage of that made available by Seaweeds immediately following harvest can have algae must be directed toward harvesting and dewatering?”. the exact ratio of water stipulated the percentages of harvestable stock allowed to to solids for marine biomass remains to be determined. laws have approaches 90% may be necessary. anaerobic digestion and fermentation. shredded or milled macroalgal biomass can go directly into either reactions As a result of growing concern about the potential or extractions. strict regulations 80 . and hydrothermal liquefaction associated biorefineries has from the outset included for bio-oils. For part of the pretreatment process.30% water content is noted to have a stabilizing harvesting may require retrieval and transport to shore. biomass prior to extraction or direct conversion have This has yet to be demonstrated on a commercially been categorized as follows (Bruton et al. operations. Hydrothermal conversions are suited for environmental consequences of harvesting natural wet biomass and become efficient at 15 . Dewatering seaweeds such as Sargassum spp.. mechanized harvesting techniques. given the issues related to sustainability and potential environmental importance as well as current cost and achievable consequences will need to be carefully evaluated. The concept of large off-shore macroalgae farms and fermentation for alcohols. which is beneficial for transportation and other Similarly.g. 2009). Dewatering to grown on off-shore rings (Buck and Buchholz. 2001). example. • Removal of foreign objects and debris.20% solids or populations of seaweed near-shore.. cultivation in land-based pond systems will processes requiring further drying (Bruton et al.85% water content (Peterson et al. final algae concentration needed for the chosen extraction will likely have less debris. about 20 .. and the amount of debris method. with higher a biofuels feedstock in Ireland (Bruton et al. 2009). In require technology appropriate for that mode of culture. Smaller particles. maintenance. scale of harvesting and dewatering. as opposed to attached to the bottom culture. for a range of dry weight content based on extraction Screening for debris is considered mandatory. significant fraction of the total amount of energy available 4.. Downstream Processing: Harvesting and Dewatering  39 . The establishment of large offshore seaweeds may alleviate 4.3 Systems Engineering pressure from near-shore environments and create market While specific process technologies have been studied. 2009): relevant scale and remains a significant challenge. 1989). attached forms that tend to stand upright. culture and end-use. 2009).. This will likely be a significant fraction of the will likely have less impact in procedures that can total energy cost of any algae-to-fuel process and a utilize whole seaweeds (Bruton et al. surface area to volume ratios.harvesters in European seaweed operations have been Milling is used to reduce seaweeds to particle sizes that are described in a recent feasibility analysis for seaweeds as \more efficiently processed. and depreciation. opportunities for products apart from fuels. although breakthroughs are still needed in each. such fermentation. sand. Floating a high tolerance or requirement for water.

47(1).H.. energy example shown below provides some food for thought balance can become an issue in systems that propose regarding harvesting and dewatering technologies.54 watt-hours/gram.B. I. 631-635. 250. Knuckey. Autoflotation harvesting of algae from high-rate pond effluents.. 612. Scragg. A. Stanley. D. Ferguson. Borowitzka. 95. Progress in Water Technology. algal biofuel production schemes at scale will of 5 watt-hours/gram if the energy content of lipids. Integrated high-rate pond algae harvesting system.C. H. (2006). V. (38). Transactions-American Geophysical Union. and proteins and the typical percentage integrated systems in order to overcome this challenge. (1975). Anderson. Response of offshore cultivated laminaria saccharina to hydrodynamic forcing in the north sea. Kwak. J.R. Water Supply: IWSA/IAWPRC Joint Specialized Conference on Coagulation Flocculation. S. 153.. Algae removal using dissolved air flotation. 85(3). (2009). Algae. (1983). Lincoln. et al. Production of microalgal concentrations by flocculation and their assessment as aquaculture feeds. 5(4). D. Ku..from algae. 133. J. Harvesting of algae grown in agricultural wastewaters.J. E. 357-394. and C. B. Micro-Algal Biotechnology. C. Rasmussen. Bracco. However. and algae with much higher energy content than available drying as a function of the volume percentage of algae today. Shales. Dublin. (2004).J. Ferguson. Lavoie. Jung.J. Edzwald. 9(3). 31(3-4). Filtration. Middlebrooks.. B.Y. 525-534. Harvesting of scenedesmus-obliquus in wastewaters-auto-flocculation or bioflocculation. Aquaculture. A. 113. bubbles. Low cost reclamation using the advanced integrated wastewater pond systems technology and reverse osmosis. flocculation and sedimentation and the belt filter press are expected to be minimal. to take algal biomass and concentrate / dry it to enable downstream processing and extraction because of the high volumes of water that must be evaporated away. Large-scale algae culture systems. Botes. 300. (2002). G.J. (1969). Possible approaches may include developing strains of hours/gram of algae for harvesting. T. Bruton. of each in algae are considered (Illman et al. Lerat. L. J. (1977).. W.F Frampton. Koopman.A. Sedimentation and Flotation.K. (1987). 6(1).S. H. Development of an attached microalgal growth system for biofuel production. E. (1991). Oswald. The energy requirements for energy intensity of harvesting and drying algae. (1995).M. and dissolved air flotation. Downing. Brown. M. (27).. 9(1). and M. (2005). Logsdon. Enzyme and Microbial Technology. 30 (7). Water Pollution Control Federation Research Journal. 11. Ireland Buck. B. 40 4. Dodd. Kim.J. H. Chen. Sustainable Energy Ireland. Zubieta. Separation and Purification Technology.. Illman. G. Lundquist. Johnson. Aquacultural Engineering. (1988).H. Comparison of dissolved air flotation and direct filtration. & Wen.L. likely need to implement innovative technologies and carbohydrates. C. de-watering. R. Removal of clay and blue-green algae particles through zeta potential and particle size distribution in the dissolved air flotation process. Jones. J.J. 674-691. coagulants. 2000). 117.. 27(10). D. N. Borowitzka. (1993). Y. S. Downstream Processing: Harvesting and Dewatering  . Vanvuuren. Water Science and Technology.. It is possible to estimate the energy requirements in watt. Green. 50(11). Agricultural Wastes.P. Water Science and Technology. J. Jones.B. 67. Applied Microbiology and Biotechnology.R. (2005).X. Butterfi. Delanoue.R. M. W. M. (2010). Curley.B. T. A quick and preliminary energy balance of vaporization of water at 0. Electrochemical technologies in wastewater treatment. A review of the potential of marine algae as a source of biofuel in Ireland. Kwon.. based on the latent heat References Bare. Water Science and Technology. J.M. C. B. Cambridge University Press.. 231. R.B. Buchholz. 852-859. Increase in chlorella strains calorific values when grown in low nitrogen media. along with innovative solutions to lower the in harvested biomass. Dissolved air flotation for the removal of algae and inorganic turbidity on a large-scale. F. Preliminary Look at Energy Balance In spite of gaps in data precluding more detailed The energy content of most algae cells is of the order analyses. Lyons. Won. E. M. 45(1). Robert. 35(3). Water Science and Technology: Water Supply. 713.F. Biotechnology and Bioengineering. (2000). Z.

Forster. White. H.and supercritical water technologies. (1984). S. Conservation. (1971).. dissolved air flotation and drum filtration techniques for harvesting sewage-grown algae. W. 24(1). Pauw. 24(5). Pan. Techno-economic feasibility analysis of offshore seaweed farming for bioenergy and biobased products. Tongkasa. A. C. 14. 4. R. Butler. Sukenik. Biomass. J. (27).G. Potential of electrolytic flocculation for recovery of micro- algae. (2001). 185. Pringle. 824. C. and Recycling. W. D. D. Mollah. Froling. (2003). Antal. P. Biotechnology and Bioengineering. et al. (1991). Y. R. Battelle Pacific Northwest Division Report Number PNWD-3931.C. Becker. 150(1-3). S. (19).S. 327.. B. Tester. N. Journal of Hazardous Materials. F. Molina Grima E. Wu. 16(1). 63-70. (1). 1987. Journal of Biotechnology Advances. Poelman. J. Water reclamation and algae harvesting. H. Huesemann. Roesijadi. Dewatering characteristics of algae-containing alum sludge..J. and J. and J.. Phoochinda. T. Yoon. Chuang. present and future perspectives of electrocoagulation.. E-H Belarbi. (1989). J. 1-7. Fundamentals. Chisti. Sharp.. Robles Medina. J.. 32-65.M. Overview of a workshop on production and utilization of commercial seaweeds . Recovery of microalgal biomass and metabolites: process options and economics. Water Pollution Control Federation Research Journal . E. A new approach to seaweed management in Eastern Canada: The case of ascophyllum nodosum.Y. China. Thermochemical biofuel production in hydrothermal media: A review of sub. G. M. Copping. Colloids and Surfaces A-Physicochemical and Engineering Aspects. 83-89.. M.A. M. K. 142. Algal autoflocculation-verification and proposed mechanism. G. Sim. W. Cah. R.P. A. Advances in Slow Sand and Alternative Biological Filtration. Y.. C. Kwon.W.G. (26). Energy & Environmental Science. (2008).C.J. Saidam. The Journal of Applied Phycology. B114. E. 51. Removal of algae using froth flotation. (2008). Oswald. 199. A... (1998). Peterson. (1996). 491–515. 1. Downstream Processing: Harvesting and Dewatering  41 . Lachance. Science and Technology. C. McGarry. Environmental Technology. Goh.P. A. G. G. Comparison of centrifugation. (2003). Tseng... E. (1988). Benemann. M. 1. M. Algae removal by horizontal flow rock filters.B. M. Resources. Ugarte. Kim. Introduction to advanced integrated wastewater ponding systems. Oh. (1997). (2004). A. Huang. 87. Mar 42.Qingdao. D.Lee. Letters in Applied Microbiology. Vogel.. Acién Fernández. 43(5). Biol. (20). Water. F. (2001). A.D. Effects of harvesting method and growth stage on the flocculation of the green alga botryococcus braunii. Shelaf.R.S.

pulverized biomass. and enzyme reactions (Mata macroalgae (Aresta. Many effective of the biomass must be disrupted prior to extraction. Laboratory-scale comparisons of process such as freezing. different Algal biofuel schemes that rely on the accumulation of harvest process operations (discussed in the Chapter 4) intra-cellular lipids are the focus of this discussion.. To operations could affect extraction processes. Extraction of Products from Algae   . and (3) solvate the >40%. microalgae and cyanobacteria may be cultivated lipid. and acid. pretreatment of (Zhang et al. which is dependant on the algal species and growth status. While macroalgae are more like traditional and cell walls may present formidable barriers to solvent feedstocks. or for the sonication for cell disruption. The use of microwaves to disrupt cells and have been carried out. shown to improve methane gas production and biosolids reduction in sludge digestion (Rittman et al. 2008.. to use elevated temperature and pressure processes that some algae-to-biofuels processes attempt to bypass the force the solvent into contact with desired biopolymers. Additionally. Virot knowledge exists for the separation of plant biomass et al. osmotic shock. base. any extracting solvent must be able to (1) fuel conversion process.. thus a high degree of concentration may be necessary before Effective mechanical disruption can help offset the need some types of extraction can begin. 2008). Lipid Extraction sonication. While many terrestrial feedstocks penetrate through the matrix enclosing the lipid material. Extraction depends on identifying the particular biological Mechanical Disruption (i.. 2010). extraction bypass schemes that attempt to engineer algal systems techniques employed must be efficient and effective. and extraction algal biofuels as competitive bulk commodity. extraction techniques require concentrated substrates. autoclaving. 2010).. algal biomass extraction in aqueous phase systems serves as a starting suffers from a lack of well-defined and demonstrated place for industrial-scale extraction operations.. A shortfall of relevant information on efficient extraction bead mills (or bead-beating). largely in the realm of laboratory-scale processes that serve analytical rather than biofuel production goals. and microwaves and suggested that microwave disruption prior to solvent The basis for lipid extraction from algal biomass is extraction is the most efficient method (Lee et al. While considerable is a promising development (Cravotto et al. Mechanical disruption can include cell homogenizers.1 Current Practices for types of oleaginous microalgae compared bead beating. though applications outside of analytical lipid extracts and preparation for conversion to biodiesel labs are unclear. Cell Rupture) component for extraction. sewage sludge with “focused pulse” sonication has been up separation challenges for extracted algal lipids. For this reason. Recent work on extraction of lipids from three different 5. prior to the application of the extraction solvents. different logistical challenges arise from access. some systems 42 5. For waste treatment. 2008). industrial-scale methods for extracting and separating of oils and lipids required for enabling biofuel production.5. application of organic solvents. 2003). can be removed from their environment at total solids (2) physically contact the lipid material. 2010) and osmotic shock. Dibenedetto. Assuming a system that requires extraction of oils and lipids from harvested biomass—as noted. 2005) et al. Non-mechanical methods include biofuel development. As such the development of any extraction process as single cells suspended in water at concentrations below must also account for the fact that the tissue structure 1% solids. extraction step by either converting whole algal biomass or Different methods can be used to disrupt the cell membrane by inducing the secretion of the desired product directly. and autoclaving of lipids and oils at larger-scale is limiting the algal-based (Mata et al. ultrasounds..e.and laboratory-scale procedures.. little is known about the scale. Lipid extraction includes the following approaches: Existing extraction techniques are mainly suitable for solvent-based extraction relying on microwaves and or analytical. but these techniques often rely increase efficiencies of vegetable lipid and oil extraction on freeze dried. as well as the be successful. 2010). that secrete products directly into the growth medium.. extraction of lipids from microalgae (Lee et al. Extraction of Products from Algae While relatively limited volumes of bioproducts are are attempting to bypass this step—the dynamics of currently produced from algal feedstocks. To produce cells without disrupting cellular functions. using solvents to “milk” algal recovery/removal of high-value products. and Barberio. This generally requires that the native structure their offshore production and harvesting.

. In general. this approach achieved by adding both hexane and water in proportions suggested that a one-step reaction that added the alcohol that create a two phase system (hexane and an aqueous (e. chloroform. solubilize. disruption of the cell Malcata. lipids: hexane/isopropanol for tissue (Hara & Radin.. other combinations lipids and cell structure. They explained lipids (i. Fatty Acid Methyl Esters (FAMES) One example is the hexane/ethanol extraction co-solvent system (Grima et al. Separation is then improved recovery of fatty acids.g. 2007). (1) the ability of a more polar co-solvent to disrupt the cell membrane and thus make it sufficiently porous and Continuing efforts along this path. the alcohol adipose tissue without prior extraction or purification for is first added as the extracting solvent. 2000). 1959). 5. these findings suggest that membrane may be necessary. acetyl chloride). biomass in a modified approach to include hexane in the reaction phase in order to avoid a final purification These results suggest that the two most important criteria step (Rodriguez-Ruiz et al. pure alcohol solutions of eliminate the need to use antioxidants to protect unsaturated greater carbon length (such as butanol) have not compared lipids. 1990). has been promoted because hexane and alcohol will It is also noteworthy that the extraction efficiency was not readily separate into two separate phases when water is improved (when water was added first). 1994). 1998). prior to their dissolution in the of co-solvents have been proposed for the extraction of monophasic system of water. it was found that when (2) the ability of a second less polar co-solvent to better applying direct transesterification using an acid catalyst match the polarity of the lipids being extracted. Consequently. (i. methanol) and acid catalyst (e. and Dyer co-solvent method.e. The sequence of solvent addition can also affect two immiscible layers.. single alcohol To increase analytical efficiency. 2007). less volatile and toxic alcohols (e. give relatively high hexane mixtures.. 2000).. agitation in the form of sonication or additional methanol. These important results have a key impact on liquid phase 1978). if not slightly better than alcohol/ Bligh and Dyer co-solvent system).. but never more than 90% of the Bligh and recoveries of volatile medium chain triglycerides. it has been demonstrated that the extraction of lipids was significantly more efficient when solvents were added in order of increasing polarity (i. The lipids mainly separate to the extraction (Lewis et al. chloroform.g.. Starting from freeze dried chloroform layer and can then be recovered for analysis. Chloroform will extract more than just the saphonifiable methanol. and hexane/isopropanol for lipids. In these applications. More. thereby improving downstream separations. It was found that the when selecting a co-solvent system to extract lipids are: entire reaction could be shortened to 10 minutes if the mixture was incubated at 100˚C under a sealed cap. hexane/ethanol for microalgae suggest that the water will form a solvent shell around the (Cartens et al. Extraction of Products from Algae   43 .g.e. water (which is not miscible with chloroform) is marine tissue that contained more than 2% lipids but added to the co-solvent mixture until a two-phase system worked well for samples that contained less than 2% develops in which water and chloroform separate into lipids... After the extraction reaction is grossly underestimated the lipid content in samples of complete. dimethyl sulfoxide/petroleum ether for yeast extraction systems applied to “wet” biomass because they (Park et al. and then water) (Lewis. the efficiency of the reaction increased when a second “less polar” solvent such as To avoid the use of elevated temperature and pressure to diethyl ether or toluene was mixed with the methanol to push the solvent into contact with the analyte (at the cost modify the polarity of the reaction medium (Carvalho and of a very high input of energy). 1-butanol and ethanol) solvents have been tried proposed the direct transesterification of human milk and (Nagle & Lemke. 2007). despite the added added. In other cases. Similarly.e. (2001) found that the Bligh and Dyer method (Bligh and Dyer. 2005).. 1996). ethanol and Direct Transesterification of Lipids into isopropanol) have been suggested in place of methanol. and methanol. their results in terms of initial contact of the biomass with lipoproteins.. and other lipid and non-lipid contaminants) nonpolar solvents weakening the association between the (Fajardo et al... This method was applied to dried microalgal well against the hexane/ethanol co-solvent system. making it more difficult for less polar solvents such microalgae (Nagle & Lemke. In general. biomass.Organic Co-solvent Mixtures Application of Organic Two-Solvent Systems for The concept of like dissolves like is the basis behind the Lipid Extraction from Microalgae: earliest and well-known co-solvent extraction procedure Iverson et al. and extract the lipids. at 100˚C for an hour under sealed cap would increase applications using pure alcohol (ethanol and 1-butanol) fatty acid concentrations measured (as compared to performed similarly. The hexane system as chloroform to contact. acetyl chloride) hydroalcoholic) that partition the extracted lipids into directly to the biomass sample and followed with heating the nonpolar hexane (Fajardo et al. In general.g. 1990). the unsaponifiable lipids such as pigments. Lepage and Roy (1984) (e.

Denery et al. the cells can be returned extractable material from various processes. break down the cell membrane.e. extraction temperature. a step that also requires energy input. extracted 85 . For to fractionate lipids from biological samples (ref). The single solvent systems at elevated temperature and ASE . 2004).e. separate out desirable by analogy. screening viability (500 . 2004) and lipids from microalgae (Schäfer. can be retained in the cell during the extraction phase (using a solvent front pumped through the sample at the This work has served as the basis for the development of appropriate temperature and pressure).. Compressed gas is used to purge the different organic phases indicated that cells remained sample extract from the cell into a collection vessel. Denery and coworkers optimized the extraction of carotenoids from Dunaliella salina All the preceding co-solvent systems. ASE of higher hydrophobicity. (2002) proposed the two-phase system of aqueous and organic phases for the selective extraction of carotenoids from the microalgae Dunaliella salina.3000 psi) conditions for short time periods and activity tests of Dunaliella salina in the presence of (5 .55% of the fatty acids extracted by rate of mass transfer and degree of solvent access the Folch method in the first solvent extraction cycle.. Their Accelerated Solvent Extraction observations were that solvents with lower hydrophobicity Accelerated solvent extraction (ASE) was first proposed reach critical concentrations more easily and in the in the mid 1990s (Richter et al. and optimize the energy cost. What remains unclear is the effectiveness of such • the elevated pressures can reduce the dielectric an approach at large scale in terms of how to handle constant of an otherwise immiscible solvent (and large amounts of biomass. must use dried Consequently.. 1996). the issue of solvent access to the material biomass. In general. depending on the solvent. and time have been optimized lipids coupled with its miscibility with methanol. By using solvents technique on 1 . 2006). some have proposed a modified technique to “milk” oils or neutral lipids from algae using biocompatible solvents and applied sonication. and showed that higher or equal extraction efficiencies remain largely bench-scale methods that are difficult (compared to traditional solvent technology) could be to scale up to industrial processes due to the actual achieved with the use of less solvent and shorter extraction solvent toxicity and the low carrying capacity of the times. In addition to improving yields and reducing loss of cell viability and extraction of membrane-bound extraction time. to all pores within the biomass matrix and. sample-solvent medium) depends upon its ability to solubilize the target ratio. reaction ASE is more efficient if extracting solvent. is enclosed in a sample cartridge that is filled with an By applying a measurement of solvent hydrophobicity extraction fluid and used to statically extract the sample based on the partition coefficient of the solvent in a two- under elevated temperature (50 . and dodecane in the presence of live microalgal cells. being extracted is as important as the miscibility of the analyte in the solvent. to selectively to their original bioreactor for continued growth and extract polar compounds from lipid-rich samples. 1996.30 g samples of dried biomass.95% pressure have gained favor for two principle reasons: of the fatty acid content in the harvested microalgae • the elevated temperature and pressure increase the compared to 44 . ASE can also be applied to remove co. This observation is a key driving force behind the consideration of solvent extraction Selective Extraction systems at elevated temperature and pressure. it is only efficient on biomass samples to that of traditional Folch method for microalgae grown containing less than 2% w/w lipids).. Accordingly. Conceptually. using the process. Extraction of Products from Algae   .. If this process can be 44 5. Hejazi et al. 2009). the polarity) to values that match the lipids.the effectiveness of the second co-solvent (i. on dairy manure effluent (Mulbry et al.10 min). (Denery et al.. viable and active in the presence of organic solvents with a log P (octanol ) > 6 and that β-carotene can be extracted ASE is applicable to solid and semi-solid samples that more easily than chlorophyll by biocompatible solvents. noteworthy in the context that ASE by definition uses non-aqueous solvents and therefore. The latter is also polarity of the lipids (Herrero et al. example. It has been proposed a technology that proposes to use solvents such as decane for the extraction of liquid extracts (Richter et al..200˚C) and pressure phase system of octanol and water. however. the effect of the solvent on uses organic solvents at high pressure and temperatures the membrane decreased and the extraction efficiency above their boiling point. The performance of ASE extraction was compared solvents (i.. free fatty acids. and production of triglycerides for biofuels production. concentrated for the extraction of triglycerides without 1998). a solid sample for both chlorophyll and β-carotene decreased as well.

water. 2007). creating additional energy use challenges.50˚C and at lower temperatures and can be easily separated. whether grown has the effect of converting lipids.applied to microalgae slurries with suspended solid extraction techniques have been used in the commercial concentrations as low as 1 wt%. In this 2006). which can moderate critical temperature of 31.379 bar (Chueng. and saponins from oil-seeds (Güçlü-Üstündağ et case. however. Some pressures of 241 . The majority of applications have used the extraction of lipids that cannot be transesterified. Use of methanol as the solvating fluid With respect to microalgae.. In this system up to subcritical temperatures. as the water is from microalgae (Mendes et al. easily separated downstream once the temperature and functional ingredients from microalgae (Herrero et al.. pressure are lowered to atmospheric conditions. products miscible Largeau. under these conditions. al. The basic premise to subcritical water extraction the extracted product remains as a liquid or solid. More recently. but other fluids used have included ethane.. as with accelerated solvent Heterotrophic Production extraction. the solvent and product can be of essential oils from plant matter (Eikani et al.. one of the more to biodiesel (see Chapter 6 for more detail). In addition.. Metzger and cooled back down to room temperature..1˚C and pressure of be difficult to separate from the desired lipids. phototrophically or heterotrophically.g.g.. thereby eliminating the need for the dewatering step. 2006). private companies have engineered algae that secrete oil into the fermentation media that can be recovered and later refined into a biofuel.. for more than two decades suitable for biofuels (e. Lipids have been selectively extracted at the high temperature and pressure become immiscible from macroalgae at temperatures of 40 . and pressure high enough to keep the liquid state (Soto and Luque de Castro.. 1992). CO2 because of its preferred critical properties (i. been completed and the extracted material dissolved into however. 2001) . is that water. as well as pigments (such as chlorophyll). However. by the same issues of energy-intensive processing and and environmental compatibility (Herrero et al. 2007). nitrous oxide. is the difficulty with designing a system at Other methods for extraction and fractionation include large scale and the high-energy load required to heat the the production of oils using heterotrophic algae. 1999). it has been applied for the selective extraction the supercritical fluid. as well as functional ingredients and lipids temperatures as discussed above. higher microalgae via supercritical processing is challenged quality of extracts. of the more important advantages described for subcritical economical production of biofuels from oleaginous water extraction include shorter extract times. One of the more attractive points to supercritical hemicellulose as a pretreatment prior to its use as a fluid extraction is that after the extraction reaction has fermentation substrate (Mok et. just below the critical temperature. higher productivity Castro et al. ease of scale-up. liquid feeds (Reverchon et al. the fluid returns to its original gaseous state while al. 2006). sulfur Subcritical Water Extraction hexafluoride. 2005). at temperatures point can be adjusted as can the time of the extraction. 2006). 1999).. Large-scale design scenario. lower costs of the extracting agent. Supercritical extraction is often employed in batch mode. 2005). The temperature and pressure above the critical liquefaction) is based on the use of water.e. this method may provide extraction of substances from solid substrates. 2006). scaling up the process that is developed mainly for analytical usage.. becomes less polar and organic compounds are more soluble than at room Supercritical fluid extraction has been applied for the temperature. There is also the added benefit of solvent extraction of essential oils from plants (Reverchon et access into the biomass matrix that occurs at the higher al. methanol.9 bar).. The potential benefits of this approach are the power of fluids above their critical point (Luque de use of standard fermentation systems. ethane. this approach significantly Supercritical Fluid Extraction reduces the capital and operating cost for an extraction Supercritical fluid extraction utilizes the enhanced solvating process. 1999). water extraction. attractive aspects is the use of water as the solvent. The technique. Some degradation. 1994).” was initially applied to whole biomass 2005). e. Supercritical fluid avoidance of expensive extraction scheme(s). 1994. triglycerides) that excludes (Brunner. Extraction of Products from Algae   45 . It can be processed using solid and compared to photosynthetic systems. 73. non-photosynthetic algae are grown using will also require a significant cooling system to cool sugars as energy source and using standard industrial the product down to room temperature to avoid product fermentation equipment (Barclay et al. via transesterification... A major constraint. however. low toxicity. a unique avenue for the selective extraction of lipids caffeine from coffee beans. originally termed “pressurized hot but the process can also be operated continuously (Brunner. as well as n-butane and pentane (Herrero et Subcritical water extraction (also known as hydrothermal al. the ability to 5. and chemical inertness (Luque de Castro et al.

et al. thereby improving the References Barclay. principal motivation behind the application of extraction but it does serve to highlight the need for innovation techniques at elevated temperatures and pressures. & Mazza. As discussed in access to the material being extracted is as important Section 4. Increasing the temperature helps to disrupt the solute- matrix interactions and to reduce the viscosity and surface tension of the water. J. J. Lipid-Fett.). Cartens.X. 2007.R. Brunner. K. Denery.maintain the integrity of the fermentation catalyst and use contact between the solvent and the solute.. A. Analytica Chimica Acta. G. a preliminary look at the energy balance as the miscibility of the analyte in the solvent. G. Pressurized low polarity water extraction of saponins from cow cockle seed. G. water can either promote the formation of emulsions in the presence of ruptured cells or participate For sustainable biofuels production. 123-129. S. Fajardo. 735–740. Comparison between extraction of lipids and fatty acids from microalgal biomass.. Supercritical fluids: technology and application to food processing. 120–126. 898-902. Very little information is available on this critical The extraction process is affected by the choice of step that is necessary before converting the algal upstream and downstream unit operations and vice biocrude into finished fuels and products. 2007. Meager. Eicosapentaenoic acid from the marine microalga Phaeodactylum tricornutum. At the cellular level. F. 1996. Extraction of Products from Algae   . reduce the pressure and temperature requirements. et al. et al.. W. M.. M. versa. J. Journal of Food Engineering. 73(8). & Malcata.R. Increased of sugar-based feedstocks.. the issue of solvent energy load. 67(1-2). Presence of Water Associated with the Biomass such as chlorophyll and non. 501(2)..2 Challenges Extraction processes can yield undesirable components.. 15(5).3. Ultrasonics Sonochemistry. 80(2). Balsevich. 2005. 175–181. This is a does not suggest that this is an insurmountable barrier.. & Abril. Journal of the American Oil Chemists' Society. Güçlü-Üstündag. to achieve sustainable commercial-scale systems. Preparation of fatty acid methyl esters for gas-chromatographic analysis of marine lipids: Insight studies. 1994. intracellular benchmark can be considered: the extraction process water can prove to be a barrier between the solvent per day should consume no more than 10% of the total and the solute. 1994. 21-33. 2008. 2004. Separation of Desired Extracts from Solvent Stream 5. 955–959. 71(9). 1025–1031. Pressurized fluid extraction of carotenoids from Haematococcus pluvialis and Dunaliella salina and kavalactones from Piper methysticum.. 80(2).. When present in the bulk Energy Consumption and Water Recycle solution. However. 6(2). 53(13). as Btu.. Grima. J. 2005. 2007. Eikani. significant downsides pressure facilitates enhancing the transport of the solvent to this approach include many of the same feedstock to the analytes that have been trapped in pores. & Rowshanzamir. Journal of Applied Phycology.H. et al. Subcritical water extraction of essential oils from coriander seeds (Coriandrum sativum L. Chief amongst is the logistical challenge of that would normally not be contacted by solvents under securing a sustainable biomass feedstock to supply to feed atmospheric conditions. Agric. Journal of the American Oil Chemists' Society. E. et al. Heterotrophic production of long chain omega-3 fatty acids utilizing algae and algae-like microorganisms. produced per day. Golmohammad. 619–630. F. Mechanical disruption can large-scale heterotrophic “algal-refinery” operations. The logistics challenges faced by the nascent lignocellulosic pressure also helps to force the solvent into matrices industry. 5049–5059.P.M. Journal of Food Engineering.transesterifiable lipids. Food Chem. In this context. Cravotto. A. Carvalho.. 46 5. the following in side reactions. 109(2). Improved extraction of vegetable oils under high-intensity ultrasound and/or microwaves. Journal of Food Engineering. The presence of water can cause problems at both ends at larger scales. Lipid extraction from the microalga Phaeodactylum tricornutum.

Hara, A. & Radin, N.S., 1978. Lipid extraction of tissues with a low-toxicity solvent. Analytical Biochemistry, 90(1), 420–426.
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natural sources: Plants, food-by-products, algae and microalgae A review. Food chemistry, 98(1), 136–148.
Iverson, S.J., Lang, S.L. & Cooper, M.H., 2001. Comparison of the Bligh and Dyer and Folch methods for
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from microalgae. Bioresource Technology, 101(1), S75-S77.
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5. Extraction of Products from Algae   47

6. Algal Biofuel Conversion Technologies
Potentially viable fuels that can be produced from algae products, and this conservation law must also hold
range from gaseous compounds like hydrogen and methane, true for the algae biorefineries of the future if they
to alcohols and conventional liquid hydrocarbons, to are to achieve significant market penetration.
pyrolysis oil and coke. Attractive targets for this effort,
however, are the liquid transportation fuels of gasoline, A large number of potential pathways exist for the
diesel, and jet fuel. These fuel classes were selected as the conversion from algal biomass to fuels. These pathways can
best-value targets because 1) they are the primary products be classified into the following three general categories:
that are currently created from imported crude oil for the
bulk of the transportation sector, 2) they have the potential 1) Those that focus on the direct algal production
to be more compatible than other biomass-based fuels with of recoverable fuel molecules (e.g., ethanol,
the existing fuel-distribution infrastructure in the U.S., and hydrogen, methane, and alkanes) from
3) adequate specifications for these fuels already exist. algae without the need for extraction;

The primary objective of this chapter is to summarize a 2) Those that process whole algal biomass
number of potentially viable strategies for converting algal to yield fuel molecules; and
biomass into replacements for petroleum gasoline, diesel, 3) Those that process algal extracts (e.g., lipids,
and jet fuel. When a fuel meets all customer requirements, carbohydrates) to yield fuel molecules.
it is referred to as “fit for purpose.” While a successful These technologies are primarily based on similar
fuel-conversion strategy will address the full range of methods developed for the conversion of terrestrial
desired fit-for-purpose properties (e.g., distillation range, plant-based oils and products into biofuels, although the
ignition characteristics, energy density, etc.), these desired compositional complexities of the output streams from
fuel characteristics are driven primarily by customer algae must be dealt with before these can be applied
requirements and are discussed later in Chapter 8. This effectively. Pros and cons of these pathways within each
chapter focuses on fuel conversion strategies from a variety of these categories are discussed below, and a summary
of perspectives to establish the current state-of-the-art, of each fuel-conversion technology is given. Inputs,
as well as identify critical challenges and roadblocks. complexity, cost, and yields are provided (where known),
and key barriers and RD&D opportunities are listed.
Several emerged the Algal Roadmap Workshop in
relation to conversion of algal feedstocks to fuels. These
are noted here to help establish a reasonable framework 6.1 Direct Production of
for the most promising concepts for algal biofuels.
Biofuels from Algae
• First, the feedstock, conversion process, and
The direct production of biofuel through heterotrophic
final fuel specifications are highly interdependent
fermentation and growth from algal biomass has certain
and must be considered together if an optimal
advantages in terms of process cost because it can
process is to be identified. As a result, accurate
eliminate several process steps (e.g., oil extraction)
and detailed feedstock characterization (including
and their associated costs in the overall fuel production
both composition and variability) is essential,
process. Heterotrophic growth also allows for maintaining
since this is an upstream boundary condition for
highly controlled conditions, which first could be oriented
the entire downstream fuel-conversion process.
toward biomass production and then oil production. Such
• Second, life cycle analysis of energy and carbon will a system can generate extremely high biomass (hundreds
be a key tool in selecting the preferred fuel conversion of grams per liter) and a high percentage of that biomass
technologies from those discussed in this chapter. as lipid (well over 50%). The system is readily scaled up
• Third, the greatest challenge in algal fuel conversion is and there is an enormous potential to use various fixed
not likely to be how to convert lipids or carbohydrates carbon feedstocks (which would bring down the cost of
to fuels most efficiently, but rather how best to use production). These approaches are quite different from
the algal remnants after the lipids or other desirable the usual algal biofuel processes that use algae to produce
fuel precursors have been extracted. All of the biological oils which is subsequently extracted and used as
petroleum feedstock that enters a conventional a feedstock for liquid fuel production, typically biodiesel.
petroleum refinery must leave as marketable There are several biofuels that can be produced directly
from algae, including alcohols, alkanes, and hydrogen.

48 6. Algal Biofuel Conversion Technologies  

Alcohols In addition to ethanol, it is possible to use algae to produce
other alcohols, such as methanol and butanol, using a
Algae, such as Chlorella vulgaris and Chlamydomonas
similar process technology, although the recovery of
perigranulata, are capable of producing ethanol and other
heavier alcohols may prove problematic and will need
alcohols through heterotrophic fermentation of starch
further R&D. The larger alcohols have energy densities
(Hon-Nami, 2006; Hirayama et al., 1998). This can be
closer to that of gasoline but are not typically produced
accomplished through the production and storage of
at the yields that are necessary for commercial viability.
starch via photosynthesis within the algae, or by feeding
sugar to the algae directly, and subsequent anaerobic
fermentation of these carbon sources to produce ethanol Alkanes
under dark conditions. If these alcohols can be extracted In addition to alcohols, alkanes may be produced directly
directly from the algal culture media, the process by heterotrophic metabolic pathways using algae. Rather
may be drastically less capital- and energy-intensive than growing algae in ponds or enclosed in plastic
than competitive algal biofuel processes. The process tubes that utilize sunlight and photosynthesis, algae can
would essentially eliminate the need to separate the be grown inside closed reactors without sunlight. The
biomass from water and extract and process the oils. algae are fed sugars, the cheap availability of which
is a key consideration for cost-effective production of
This process typically consists of closed photobioreactors biofuels; these sugars are themselves available from
utilizing sea-water with metabolically enhanced renewable feedstocks such as lignocellulosic biomass,
cyanobacteria that produce ethanol or other alcohols while in a pressure and heat-controlled environment. This
being resistant to high temperature, high salinity, and process can use different strains of algae to produce
high ethanol levels—previous barriers to commercial- different types of alkanes; some algae produce a mix of
scale volumes (Hirano et al., 1997). There have been hydrocarbons similar to light crude petroleum. These
reports of preliminary engineered systems, consisting of alkanes can theoretically be secreted and recovered
tubular photobioreactors (Hirano et al., 1997). One key directly without the need for dewatering and extraction,
aspect of the system is that a source of cheap carbon, such but more often are associated with the algae and thus must
as a power plant, is typically used to supply CO2 to the be recovered through dewatering and extraction. With
bioreactors to accelerate the algae growth. An example further processing, a wide variety of fuels can be made.
of this process technology links sugar production to The process of growing the algae heterotrophically may
algal photosynthesis. There are claims that this process present some advantages over typical photoautotrophic-
may consume more than 90% of the system’s CO2 based technologies. First, keeping the algae “in the dark”
through photosynthesis, wherein a portion of the carbon causes them to produce more alkanes than they do in the
in these sugars is converted into ethanol. The ethanol presence of sunlight. While their photosynthetic processes
is secreted into the culture media and is collected in are suppressed, other metabolic processes that convert
the headspace of the reactor, purified, and stored. sugar into alkanes can become active. Second, some have
shown the growth rate of the algae to be much higher
This technology is estimated to yield 4,000 - 6,000 gallons than traditional methods (Xu et al., 2006). This is possible
per acre per year, with potential increases up to 10,000 because instead of getting energy for growth from sunlight,
gallons per acre per year within the next 3 to 4 years the algae get concentrated energy from the sugars fed into
with significant R&D. It is theoretically estimated that the process. These higher cell concentrations reduce the
one ton of CO2 is converted into approximately 60 - 70 amount of infrastructure needed to grow the algae, and
gallons of ethanol with this technology. With such yields, enable more efficient dewatering if is actually required.
the price of captured CO2 becomes significant, and may
require a price less than or equal to $10 per ton to remain Using algae to convert cellulosic materials, such as
cost-competitive. Further breakthroughs that enable more switchgrass or wood chips, to oil may have an advantage
efficient production systems and the development of new over many other microorganisms under development
process technologies may be critical in terms of long-term for advanced biofuel production. When lignocellulosic
commercial viability. Scaling of these systems to large- biomass is pretreated to allow for enzymatic hydrolysis
scale commercial biorefineries will also require significant for production of sugars, many toxic byproducts are
advances in process engineering and systems engineering. released including acetate, furans, and lignin monomers.
Metabolic pathway engineering within these algae, enabled In most other processes, these toxic compounds can
by metabolic flux analysis and modern genomics tools, may add process costs by requiring additional conditioning
further help in producing a commercially viable organism.

6. Algal Biofuel Conversion Technologies   49

social acceptance.steps or the concentration of biomass hydrolysate There are four major categories of conversion in the conversion step. place in their presence. competitive inhibition of photosynthetic hydrogen production by CO2. can result in a wide range of and dark-fermentation (see Chapter 2). but algae. and detailed side-by-side comparison was found in 2003). in that it is extremely fast. gasification. it appears fast pyrolysis to create bio-oil.. where finely on economic considerations. There are several reports on the pyrolysis of algae in the scientific literature (Demirbas. 50 6. It is unclear exactly how much more difficult and fucoidin. The thermochemical treatment of indirect biophotolysis. 2006. This is one area where algae have a major advantage over other biomass sources because it exists fundamentally in small 6. Brazil. the whole algae can be processed into fuels bio-oil from pyrolysis is not a commercial product at the instead of first extracting oils and post-processing. Although synthetic diesel photosynthetic activity. such as mannitol. Algal Biofuel Conversion Technologies   .500˚C for development of a robust hydrogen infrastructure less than 2 seconds.. and significant dehydration crops. Even with the increased methods benefit from reduced costs associated with interest in converting biomass into liquid transportation the extraction process. products. depending on the reaction parameters. Algae may prove to be more technologies that are capable of processing whole resistant to these compounds and sugar conversion. These current time (Bridgwater. certain species are suitable for gasification (Ross et must be performed upstream for the process to work al. 2003.e. These include the restriction of photosynthetic conversion methods. biohydrogen) substance by heating. and the ground feedstock is quickly heated to 350 . anaerobic digestion. biological hydrogen production can be considered a viable 2006). to other biomass sources due to uncertainties in the Lipid content of a variety of macroalgal species is typically ability to dehydrate the feedstock. efficiently.. feedstocks must be ground into fine particles. 2004).. including direct biophotolysis. photo-fermentation. and the United States. improvement in hydrocracking. McDermid & Stuercke. higher efficiency can be achieved development of advanced photobioreactors. gradient. produce a suitable feedstock for generating efficiency through genetically engineered algae and/or the standard diesel fuel. the algae. too low for conversion to biodiesel. but also by the so-called “flash pyrolysis” technology. typical biomass throughout the country.2 Processing of Whole Algae units and has no fiber tissue to deal with. is a relatively new process (Bridgwater. fast There are several challenges that remain before heating rates. Pyrolysis is the chemical decomposition of a condensed Biological production of hydrogen (i. and the added benefit of being fuels.1). Hydrogen The production of hydrogen derived from algae has Pyrolysis received significant attention over several decades. or other biomass. with some hydrotreating and not only on research advances. represent the main macroalgae biochemical it would be to convert algae into a bio-oil compared feedstocks for conversion to liquid fuels (McHugh. especially amenable to processing a diverse range of algae. Several pilot plants for fast pyrolysis of biomass have been built in the In addition to the direct production of biofuels from past years in Germany. though from algae. although the scientific literature. 2003). Macroalgae has specifically received some attention as a gasification feedstock and initial work shows that while A significant roadblock in using pyrolysis for algae some key differences exist as compared to terrestrial conversion is moisture content. 2008). Polysaccharides. The bio-oil has an advantage that it can enter directly The future of biological hydrogen production depends into the refinery stream and. and competitive drainage of fuel cannot yet be produced directly by pyrolysis of algae. 2003). 2007). and moderate temperatures (Huber et al. 2004).e. and supercritical processing (Exhibit 6. It does not involve reactions with technologies provide a wide range of approaches to oxygen or any other reagents but can frequently take generate hydrogen. Also. Miao and Wu. degradable alternative liquid called bio-oil can be produced. For flash pyrolysis. Liquid product yield tends to favor short residence times. Pyrolysis has one major advantage over other technology. i. requirement for Pyrolysis is being investigated for producing fuel from bicarbonate binding at photosystem II (PSII) for efficient biomass sources other than algae. with hydrogen production by accumulation of a proton reaction times of the order of seconds to minutes. algae: pyrolysis. laminarin. at least some level of dewatering is still required. no comprehensive less than 5% of total dry weight (McDermid & Stuercke. a electrons by oxygen in algal hydrogen production.. It appears that pyrolysis will concentrations approaching 20% are reported in some not be cost-competitive over the short-term unless an species (Chu et al.

2007. bio-syngas is a versatile feedstock and it studied. and it of compounds and compositions depending on the is reasonable to expect that once water content is adjusted type of algae and upstream processing conditions for. 2006). there are also significant gaps in the information available about the specifications for Conversion of bio-syngas has several advantages over other converting algal bio-oil and the resulting products. the process more flexible.inexpensive dewatering or extraction process is also Gasification developed. Work also needs to be performed to understand the can be used to produce a number of products. it is expected flexible way to produce different liquid fuels. The synthesis While algal bio-oil may be similar to bio-oil from of mixed alcohols using gasification of lignocellulose is other biomass sources. and impurities) are cleaned and upgraded to usable algal growth conditions (Miao and Wu. of algae can be tailored by carefully controlling the H2O. Balat.. 2009). Srinivas et al. Yung et al. Exhibit 6. Additionally.1 Schematic of the potential conversion routes for whole algae into biofuels Algae Anaerobic Supercritical Pyrolysis Gasification Digestion Fluids SynGas Liquid or Fischer Higher Alcohol Vapor Fuel Tropsch Synthesis Catalytic Catalytic Upgrading Upgrading Transportation Liquid MeOH Biogas Fuels . FTS is also a relatively demonstrated that the bio-oil produced by pyrolysis mature technology where the syngas components (CO. 1994). Another research paper comparatively straightforward. H2. so the bio-oil may be more investment required. it may have a different range relatively mature (Phillips... address the issue of availability easily transported throughout the upgrading process. the gasification of algae to these biofuels would be (Zhang et al. It may be possible to feed development of stabilizers for the viscosity of the bio-oil algae into a coal gasification plant to reduce the capital and acid neutralizing agents. 6. liquid fuels through a water-gas shift and CO hydrogenation (Okabe et al. First and foremost.Liquid or Hydrogen Gas Hydrogen Fuels EtOH. 2009. etc. Algal Biofuel Conversion Technologies   51 . The methods.. primarily that only incremental improvements will occur and a through Fischer-Tropsch Synthesis (FTS) or mixed breakthrough in conversion efficiency appears unlikely. making detailed molecular composition of the resulting bio-oils. it is possible to create a wide optimal residence time and temperature to produce different variety of fuels with acceptable and known properties. algal bio-oils from different feedstocks need to be carefully Additionally. 2007. research is needed on the catalytic conversion possibility to integrate an algal feedstock into an existing of the resulting algal bio-oils. CO2. 2004). Unfortunately. since pyrolysis is already a Gasification of the algal biomass may provide an extremely relatively mature process technology. Another area of interest is the thermochemical infrastructure. alcohol synthesis of the resulting syngas. Another advantage is the Additionally.

36 kJ g−1.. the most significant problem al. with the exception of any upstream process steps that may be Direct hydrothermal liquefaction in subcritical water a source of contaminants which will need to be removed (defined as water held in a liquid state above 100oC by prior to reaching the FT catalyst.330 mPas and a calorific value of Tar formation can be minimized or avoided via entrained. While this technology requires sub-millimeter a net energy producer from the overall process energy sized particles. and improve the process for indirect gasification of algae. 2005). it is difficult to reach such braunii as a feedstock has also been reported. France in 2006. and there are a few reports that used algal biomass with catalytic cracking of the tar is currently being as a feedstock.. Liquefaction of algae is two successive reactors. The key roadblocks to using FTS for algae are thought Liquefaction to be similar to those for coal (Yang et al. Liquefaction of Dunaliella tertiolecta built in Finland in a joint venture of the Technical with a moisture content of 78. with oxygen at high temperature. Even though FTS is a mature technology. 2005).. Also. In the same flow gasification at high temperatures (Hallgren et al. algae may have a unique advantage balance. considered a promising technological approach but due temperature gasifier that broke down the biomass into to limited information in hydrothermal liquefaction volatiles and solid char. it is necessary to determine the optimum conditions 52 6. as high as 75%. 2006). A solvent tar removal demonstration was (Minowa et al.4 wt% produced an oil Research Centre of Finland (VTT). However. Prior work in direct liquefaction of biomass was very A demonstration plant for gasification of wood chips active. Additionally. Neste Oil. The tar-rich gas was then passed of algae to date. The tars cause coking of the shortened time scales. Another approach for tar-free syngas was liquefaction technique was more effective for extraction demonstrated in a pilot plant in Freiberg. It would be desirable to efficiency through economy of scale. The main product of this liquefaction process is a “bio-crude” • Fluidized-bed gasification and catalytic reforming that typically accounts for 45% wt. The oil obtained at a reaction installed in a plant in Moissannes. The pilot plant used dioxide (Aresta et al. since determine the feasibility of using the oxygen generated FTS is an exothermic process. of the feedstock on a • Fluidized-bed gasification and solvent tar removal dry ash free basis and has energy content that is comparable • Fluidized-bed gasification and to diesel and can be upgraded further. These technologies harness the high synthesis catalyst and any other catalysts used in the activity of water in subcritical environments that is capable syngas cleanup process and must be removed.. FTS tends to require applying pressure) is a technology that can be employed to production at a very large scale to make the process convert wet algal biomass to a range of liquid fuels (Patil et efficient overall. First. 1995). Algal Biofuel Conversion Technologies   . there are still several areas that should be investigated and require R&D. There are reports subsequent thermal tar cracker (Goudriaan et al. The a small size with other biomass sources and doing so algal biomass was processed by liquefaction at 300˚C.. usually requires pretreatment. 2000) that claim the thermal efficiency. comparable to those of fuel oil. The four of decomposing the algal biomass into smaller molecules basic mechanisms to deal with tar-related problems are: of higher energy density or more valuable chemicals. of microalgal biodiesel than using the supercritical carbon built by Choren Industries GmbH. it would be useful to leverage dewatering process with a regenerative heat exchanger.. Germany. This technology is a representation of the natural with FTS is the cost of clean-up and tar reforming. but certain species of catalyzed by sodium carbonate (Sawayama et al. 1995). • Entrained-flow gasification at high temperature defined as the ratio of heating values of bio-crude products and feedstock plus external heat input. more research in this area is needed through an entrained-flow gasifier where it was reacted before it can become a commercially viable option. A maximum oil yield of 64% using Botryococcus in this process.. report it was concluded that liquefaction technique was 1993). temperature of 340˚C and holding time of 60 min had a viscosity of 150 .. (Raffelt et al. 2008). and yield of about 37% (organic basis) at 300˚C and 10 MPa Stora Enso. algae may not require pretreatment due to their inherent There have also been comparative studies that report the small size. The first reactor was a low. Typically. it should be possible to use by algae for use in the gasifier to reduce or eliminate the some of the heat for drying the algae during a harvesting/ need for a tar reformer.for dedicated biomass plants. Tars geological processes known to be involved in the formation have high molecular weight and can develop during of petroleum-based fossil fuels realized over greatly the gasification process. ongoing syngas-to-ethanol research using cellulosic.

there are no organic solvent applied in the processing of algae. There is to the transesterification of the algal oil extracts.4 ml/g-d of biogas can be realized using a two- efficiency. cost. While the occurrence of A recent study indicated that biogas production levels water in the reaction medium appears as a factor in process of 180.. gal vs. Additionally. in particular. which supercritical process to produce biodiesel from vegetable 6. an obvious and critical link between the type of extraction process used and the product composition.. with a conversion cost-effective methodology. however. Vieitez et al. is to demonstrate are selective. triacylglycerides. and this be mitigated through the addition of a transesterification technique has been demonstrated to be extremely powerful conversion process. 2008. 2006). In for example. 2009). Additionally. raw. oil extract. unprocessed algae and that there is no negative thereby increasing the efficiency of the process. further study will also 6. the typical mode of biofuel production from algae. and as such. Supercritical fluids The clear immediate priority.. e. it is envisioned as being applicable for the processing biofuel production. could become cost-competitive with fossil fuels. the decomposition of fatty acids is the main stage anaerobic digestion process with different strains factor that limited the attainable ester content (Vieitez et of algae. 2009). and as such may be a process is capable of tolerating water. the overall algal biorefinery in the extraction of other components within algae (Mendes.Supercritical Processing oils in one step using alcohols (Anitescu et al. Similar results have been Fernández et al. it has been demonstrated that this extraction. 2007). supercritical methanol in the United States (Hanssen et al.. In the case of combined extraction and transesterification of algae. with similar yields and efficiencies at a Extraction is efficient at modest operating temperatures. including drying. $0.51/gal). $0. It was found that the processing cost of the proposed Supercritical processing is a recent addition to the portfolio supercritical technology could be near half of that of the of techniques capable of simultaneously extracting actual conventional transesterification methods (i. In the case of catalyst-free supercritical ethanol costs associated with algal biofuels. it must be demonstrated that this process can product stability and quality. This supercritical transesterification approach can also be applied for algal oil extracts.. transesterification. It also remains to be seen whether the processing of whole algae in this The conversion of extracts derived from algal sources is fashion is superior.. key obstacles that are responsible for the current high 2004). conversion of vegetable oils. therefore. It is. in place. and efficiency. theoretically possible Supercritical fluid extraction of algal oil is far more efficient that if the other upstream algal processing costs could than traditional solvent separation methods. in terms of yield.. further work should be focused on the using strains not optimized for lipid production. level that can be scaled to commercial production. ensuring maximum particular. The supercritical extraction process can be coupled with a transesterification reaction scheme to enable a “one Anaerobic Digestion of Whole Algae pot” approach to biofuel production (Anitescu et al. a consequence of temperature and pressure conditions where algae are grown under uncontrolled conditions used in this study.. If this approach can be modified observed for supercritical methanol processing of vegetable for the use of microalgae. Algal Biofuel Conversion Technologies   53 .. Although it has been only demonstrated for the of macroalgae. 1987). fundamental and exhaustive understanding of the different at least in the case of vegetable oil processing. In this process variant. such as Laminaria hyperbore and simultaneous extraction and transesterification of vegetable Laminaria saccharina. it may be very effective oils (Hawash et al. effect of milder process conditions. The use of or ethanol is employed as both the oil extraction medium this conversion technology eliminates several of the and the catalyst for transesterification (Warabi et al. 2008).3 Conversion of Algal Extracts be needed to avoid saponification. The most common type of algal extracts under One economic analysis has been conducted based on a consideration are lipid-based. a The economics of supercritical transesterification process. and fuel conversion. 2009). supercritical tolerate the complex compositions that are found with fluids can be used on whole algae without dewatering. thus providing high purity and product that these supercritical process technologies can be concentrations. Several studies have been yield similar to that of the anhydrous process in the carried out that demonstrate the potential of this approach. impact due to the presence of other small metabolites. and one that receives scant attention of algae. lower reaction temperatures.26/ and converting oils into biofuels (Demirbas. The production of biogas from the anaerobic digestion 2008). with a methane concentration of 65% (Vergara- al. either whole or its residues in the extract or spent biomass (Demirbas. have been types of inputs to the conversion technologies must be shown to be very favorable for large-scale deployment. is an interesting mode of gaseous oils. Because decomposition was for situations like integrated wastewater treatment. 2008). at less than 50˚C.e.g.

a drastic reduction in the quantity of co-products the acidic catalysts are less sensitive to the presence of and a short separation time are obtained (Lertsathapornsuk Exhibit 6. In triacylglycerols extracted from algae to FAMEs (fatty order to compensate for this. Biodiesel is then separated processing variants that appear promising. Transesterification (Alsalme et al. Though acid catalysts have these advantages.. 2008). 2008). 2006). catalyzed methanol approach. Olefin Diesel Diesel Aromatics 54 6. acid methyl esters).. strength resulted in lower pretreatment temperatures. enhancing the product recovery be employed to convert algal extracts. there are other solution and mixing well. This technology is relatively mineral acids due to their higher acid strength (L. it was can be performed via catalytic or non-catalytic reaction shown that HPA-catalyzed transesterification of vegetable systems using different heating systems that are required oil achieves higher reaction rates than conventional to initiate the reaction. Diesel. This section discusses water and free acids. (Exhibit 6. HCl is accelerated and requires shorter reaction times. and therefore. (Ataya et al. 2007).. they are not currently preferred due to their lower activity than the conventional transesterification Chemical Transesterification alkaline catalysts. 2008). Higher temperatures and longer reaction The transesterification reaction is employed to convert times are. Algal Biofuel Conversion Technologies   . 2008). therefore. 2009).2). the reaction soluble bases by liquid acid catalysts such as H2SO4. In addition to the classic base.. heteropolyacids (HPA). biochemical.. it has been shown that One recommended research focus would be to further transesterification of algal oil can be achieved with ethanol develop these homogeneous catalysts to tolerate the and sodium ethanolate serving as the catalyst (Zhou & contaminants expected to be present in algal extracts. 2008. heating system that can be used to enhance the kinetics of transesterification involves the use of microwaves (Refaat Another route is found in acid-catalyzed transesterification & El Sheltawy. have been shown to lower the displacement of an alcohol group from an ester by required temperatures and decrease the reaction times another alcohol (Demirbas. Kerosene. The products of these reactions are typically separated by adding ether and salt water to the In addition to alternative catalysts.can be converted into biodiesel. mitigate saponification chemical. As a or H3PO4 is also considered an attractive alternative as result. When the transesterification reaction reactions (Wahlen et al. and catalytic processes that can and emulsification. Recently. Cao et al. The apparent higher activity of certain standard” in the conversion of vegetable oils into biodiesel HPAs with respect to polyoxometallates of higher (Hossain et al. which is simply a process of such as H3PW12O40/Nb2O5.. 2008). The replacement of is carried out in the presence of microwaves.2 Schematic of the various conversion strategies of algal extracts into biofuels Algal Oil Extracts Chemical Enzymatic Catalytic Transesterfication Conversion Cracking Liquid or Biodiesel Vapor Fuel (ME) Catalytic Catalytic Upgrading Upgrading Gasoline. Xu mature and has been demonstrated to be the “gold et al. Boocock. An alternative from the ether by a vaporizer under a high vacuum. generally required as a result.

Although very effective and relatively economical. 2007)... Again. indicates that the use of this co-solvent mixture in the In al.. they have not been demonstrated at large scale mainly due to the relatively high price of lipase and its short operational life caused by the negative Catalytic Cracking effects of excessive methanol and co-product glycerol. reaction temperatures. These preliminary results indicate that that a co-solvent mixture may be critical in defining microwave processing may be cost-competitive with the the optimal reaction medium for the lipases. 2006). in many cases.. Other important issues that need further exploration and require removal of alkaline catalyst from the product are developing enzymes that can lyse the algal and treatment of alkaline wastewater. Use of biocatalysts cell walls. much R&D is needed in the discovery. In particular. 2008).. have been proven as a viable means of triacylglycerols for biodiesel production (Su and Wei. 2008). ultrasonic waves cause (b) high reaction rates and conversion can be obtained. Liao et al. 2008. 2008). as with other approaches. novel enzymes with desired characteristics that are more suitable for industrial applications (Guncheva et al. these catalysts still require purification One critical area that needs to be addressed is the solvent and removal from the product stream. (a) both the negative effects caused by excessive methanol and co-product glycerol can be eliminated completely. This work more mature conversion processes currently available. It is capable of producing these reactions in a variety of estimated that industrial-scale ultrasonic devices can allow environments and on different types of oil feedstocks for the processing of several thousand barrels per day. catalysts may be used to enhance the enzymatic biodiesel production has several advantages: impact of microwave irradiation (Yuan et al. Lewis acid catalysts. production. both types of sites could be solvents to varying degrees to enable cost-effective present and it is not easy to evaluate the relative importance biofuel production (Fang et al. Enzyme immobilization may also play Biochemical (Enzymatic) Conversion a key role in developing an economic method of Chemical processes give high conversion of triacylglycerols biocatalytic transesterification (Yamane et al. inexpensive (McNeff et al. There have been of the two types of sites in the overall reaction in terms some recent reports of using a solvent engineering of efficiency and cost. and (d) the operational stability of the catalyst is the mixing and heating required to carry out the high. Bioprospecting for will require further innovation to reach production levels the enzymes in extreme environments may produce sufficient for massive and scalable biofuel production.. it has been noted 6. converting triacylglycerols into fatty acid methyl esters. sugars. but (Lopez-Hernandez et al.. The presence of development of immobilized heterogeneous and/ solvents is sometimes necessary to enhance the solubility or homogeneous catalysts that are very efficient and of the triacylglycerols during the extraction process. 2003). 2007). optimizing specific enzyme activity to (lipases) in transesterification of triacylglycerols for function using heterogeneous feedstocks. such as method to enhance the lipase-catalyzed methanolysis of AlCl3 or ZnCl2. which increases and temperature tolerance of the enzymes in order to the overall costs. one of the most transesterification process (Armenta et al. One potential solution to this is the enable efficient biocatalytic processing. Hence the process of transesterification can run inline rather than using the To that end. and 2008). time-consuming batch process used in traditional base. This cavitation provides simultaneously reuse. Acid and basic catalysts and the enzymes used in the downstream conversion could be classified as Brönsted or Lewis catalysts. catalyzing nucleic acid hydrolysis. Thus significant roadblocks to demonstrating the validity of using an ultrasonic reactor for biodiesel production this approach lies in the conversion of algal oil extracts drastically reduces the reaction time. Algal Biofuel Conversion Technologies   55 . 2008)... increasingly attractive. 2005). process must be able to function in the presence of these However. at a commercial scale and at competitive prices. In the ultrasonic reactor method. and optimization of enzymes that are catalyzed transesterification (Stavarache et al. Although enzymatic approaches have become converting lipids into a suitable diesel surrogate. the reaction mixture to produce and collapse bubbles (c) no catalyst regeneration steps are needed for lipase constantly. 1998). engineering. and energy input (Kalva et al. defining biodiesel production addresses these problems and necessary enzyme reactions (cell wall deconstruction offers an environmentally more attractive option to the and autolysin). The transesterification catalysts presented above are These factors must be addressed before a commercially very strong and relatively mature in the field of biofuel viable biochemical conversion process can be realized. converting carbohydrates into conventional processes (Svensson and Adlercreutz. to their corresponding esters but have drawbacks such as being energy-intensive. difficulty in removing the glycerol.. 2008).

for whale oil. and give high glycerol yield with high activities.. such as tehtrahydrofuran. Fatty acids are well suited to conversion to diesel and jet fuel with few processing steps. using autoignition characteristics (as measured by octane number HTiNbO3 as the catalyst. jet fuel. The catalysts are active feedstock. be completed before these catalysts are fully understood. and x is an and process steps. temperatures of about 170˚C). the predominant feedstock for the industry is crude oil. the with TiVO4 being the most active (Cozzolino et al.The presence of a co-solvent. is largely a process of eliminating oxygen and temperatures than the ones currently used (~220 . and now it is apparent that it is beginning to These catalysts are Lewis acids. probably due to the “renewable” or “green” if it is derived from a biological hydrophobicity of their surface. but have essentially in the esterification reaction. the process development but must resist poisoning and the feedstock is considered fungible with petroleum and leaching of active components. Other based analog. and diesel are generally described as amounts of free fatty acids and water. which translates to very costly plant design of those operations already in place: thermal or catalytic and construction requirements. Conversion to Renewable Diesel. presented above seem to be good candidates for industrial and catalytic structural isomerization. gasoline. One particular concern is the stability and longevity of Various refiners and catalyst developers have already the catalysts in a representative reaction environment. begun to explore the conversion of vegetable oils and waste animal fats into renewable fuels. reducing the concentration the same performance specifications as the petroleum- of free fatty acids in non-refined oil or in used oil. 2009). maximizing the final energy content. and they are better defined by their integer not greater than 7. hydrophobic (at reaction return to biological feedstocks to keep the pipelines full. heterogeneous catalyst that has comparable activity Conversion of biological feedstocks to renewable fuels. 56 6. obtained in a fixed-bed reactor at 200˚C and 35 bar. Vanadate metal compounds or cetane number). initial and final boiling point. catalysts derived from the titanium and modification of the components in crude oil to yield compound possessing the general formula ATixMO. On the other hand. Typical biological molecules One of the most difficult challenges is finding an ideal have very high oxygen contents as compared to crude oil. A typical diesel must meet a multitude of performance specifications FAME yield of 91% and glycerol yield of 91% were that include volatility. and insoluble. As noted in chapter 8. oil industry has begun to cast a wider net and has spent 2006). From a refinery’s At these temperatures. and Jet Fuel efficiencies of up to 98% (Soriano et al. active catalysts during transesterification.240°C). It is worth noting that the Double-metal cyanide Fe-Zn proved to be promising petroleum industry began by developing a replacement catalysts resulting in active transesterification of oil. straight chain alkanes are poor starting materials for gasoline because they provide low octane numbers. in comparison to the homogenous catalyst at lower therefore. CaO. The fuel products are a which A represents a hydrogen atom or an alkaline metal mixture of components that vary based on input stream atom. In this way. demanding additional isomerization steps or high octane blendstocks. catalytic hydrocracking and hydrotreating. The catalysts obtained are stable and molecules. and cloud point. separation In another example. M a niobium atom or a tantalum atom. Many of the catalysts cracking.60 bar). Algal Biofuel Conversion Technologies   . All of the processes that take place in a modern petroleum refinery can be divided into two categories. fuels is high energy content which is a function of a near-zero oxygen content. were employed in vegetable oil performance specifications than by the sum of specific transesterification. This catalyst is also more active than HTiNbO3. such as oil shale and tar sands.. a great deal of resources developing additional inputs producing the same yields with lower residence times. There remain significant can be used for the production of typical fuels without fundamental studies and unanswered questions that must disruptive changes in processes or infrastructure. can play a vital role in achieving high conversion Gasoline. This process has already provided the renewable jet fuel blends (derived from oils obtained from jatropha and algae) used in recent commercial jet test flights. the process pressure is high perspective. the ideal conversion process would make use (40 . they can be used even with oils containing significant Gasoline. Moreover. such as biomass or plant oil. in an assortment of end products. A major characteristic of petroleum-derived catalyst examples include MgO. Although are stable. jet fuel. flash point. and Al2O3.

Catalysts in any recoverable polysaccharides into biofuels. the attack on the oxygen-bearing carbon atoms will minimize organic fractions of the algae remaining after oil extraction the amount of CO and CO2 lost. There will also likely be a high metals (especially magnesium) from chlorophyll. or One other critical aspect in developing a conversion carbon monoxide.. In particular. It will amount of polysaccharides and other oligosaccharides be necessary to optimize both the level of purification of present in the algal remnants that are well suited for algal lipid as well as the tolerance of the catalyst for the traditional fermentation into ethanol and other biofuels. The liquid effluent and nitrogen heteroatoms) without becoming poisoned. The biogas product typically contains 60% found within the petroleum stream (e. carbon dioxide. Refinery catalysts have also been developed to the algae has been harvested. and reduce double bonds to yield technology that derives benefit from every potential hydrocarbons. Referred to as hydrotreating. a chemical reductive process).g. contaminants to arrive at the most cost-effective process. once H2 used. 6. this process will convert the carboxylic Remnants after Extraction acid moiety to a mixture of water. and for recycle to the culture. nitrogen from extracted proteins. contains soluble nitrogen from the original algal proteins. Algal Biofuel Conversion Technologies   57 .4 Processing of Algal (basically. little if any pretreatment function within a certain range of chemical components is required. current use have been optimized for existing petroleum feedstocks and have the appropriate specificity and activity Anaerobic digestion can be effectively used as a means to carry out the expected reactions in a cost-effective of producing biogas from algae and algal remnants after manner. Crude algal oil may contain high levels of phosphorous the nitrogen can be recovered in the form of ammonia from phospholipids. It will be desirable to tune catalysts such that the extraction (Ashare and Wilson. and sulfur methane and 40% CO2 by volume.Algal lipids can be processed by hydrothermal treatment 6. In addition. Glycerin can be converted to propane input is the conversion of algal remnants after which can be used for liquefied petroleum gas. 1979). as well as the fermentation of renewable fuels is catalyst development. This includes the anaerobic digestion of algal remnants The primary barrier to utilizing algae oils to make to produce biogas. conversion of algal feedstock into fuel. metals. as well as the amount of are amenable to anaerobic digestion.

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Co-products   61 .. generation will inevitably compete for carbon. This chapter will of algal biomass becomes feasible (Mooibroek et al. novel technologies with increased coloring substances. all primary components of algal biomass – carbohydrates. primarily by the derived from microalgae and cyanobacteria. without invoking competing co. and can be produced at a volume of roughly 100 . The use of terms such as “high volume” or “high value” This chapter will also address within the context of the can be extremely subjective. Indeed. these include products for human and animal region according to the cost of the raw material (Willke and nutrition. etc. reductant. Exhibit 7. high-value and high-volume chemicals from biomass raw material must be considered as a means to amino acids. this chapter will focus on co-products must be correspondingly low since their the non-fuel co-products. $0. fertilizers and soil conditioners.1 An overview of the biorefinery concept Biorefinery Aquaculture Primary Refinery Industry Conversion Carbohydrates Raw biomass. 2007) address these options and discuss how some of them and must be considered in determining the economics of are better opportunities as they will not readily saturate the process. proteins and a variety of inorganic and complex organic molecules – 7. to be developed to handle the large amount of waste that biodegradable polymers. For the purposes of the Roadmap.1). Heat. the concept of Under the biorefinery concept (Exhibit 7. as a “high value” product biorefinery the possibility of coupling biomass cultivation to a fine chemical producer might be well over several with CO2 mitigation (for carbon credits) and wastewater dollars/lb. but considerably under a dollar for a commodity treatment (for nutrient removal) to provide additional producer. fats (oils). and efficiencies and reduced environmental impacts may have a variety of specialty products such as bioflocculants.30 . and energy from photosynthesis. a reasonably benefits to the technology. pharmaceuticals. the generation of other been discussed (see Chapter 6). the production a biorefinery for utilization of every component of the of industrial.7.2. and stable isotopes for research purposes. anti-oxidants.$1. Vorlop. corresponding markets in the long term. valued chemical is defined as one that will cost roughly products. As summarized economics of the system. hormones. wastewater. CO2 Products and Complex Fats residues from Substances conversion Special substances: processes pigments. enzymatic or microbial conversion (see Chapter 6). and nitrogen-containing components enhance the economics of the process. aromatic essences.500 x106 lbs/yr. 2007 7. cosmetics. The topic of polysaccharides. and they may vary from region to in Exhibit 7.00/lb. Moreover. either through Microalgae and Cyanobacteria chemical. Inorganics Proteins enzymes. electricity Energy Modified from Kamm and Kamm. The nature of the end products and of the technologies A large number of different commercial products have been to be employed will be determined. 2004). is predicted to be generated by the process. Using appropriate technologies.1 Commercial Products from can be converted into different products. flavors. Co-products The “guiding truth” is that if biofuel production is conversion of algal biomass to other biofuels has already considered to be the primary goal. dyes. poly-unsaturated fatty acids. glycerol.

these existing markets (and associated Spirulina. 2004. γ-linolenic acid (GLA). horses. not commodity acids determine the nutritional value of microalgal products. etc.By definition. 280 million US$. weight gain. have also been produced by microalgae (Borowtizka.g. Although small.. and EPA by microalgal biomass as a human health food supplement Nannochloropsis. The most very large potential markets are developed. 500 t/yr Aphanizomenon. Although it is clear that co-products may other toxic elements. ca. Anti-Oxidants The market. poultry. the goal of the industry is to produce animal feed. is expected to expand significantly. Radmer. al. Skeletonema. and far on a commercial scale by microalgae. ca. 2. commonly considered PUFAs are arachidonic acid (AA). Radmer. crustaceans (shrimp). and soil fertilizer. estimated Therein lies a major conundrum associated with the to be about 300 million US$. Scenedesmus.000 t/yr Spirulina. This situation. Co-products   .. The market for microalgal animal feeds. 1990. Isochrysis. particularly given the fact that algae transportation fuels below their market price. Phaeodactylum. from 300 to 3. Chlorella. 2006). significant amounts of lead. gas from coal-fired power plants that will be used co-products of higher value in the marketplace must be to supply carbon dioxide to the cultures will contain pursued in order to offset the costs of production of algal. having presently a retail value of 1. Spolaore et (e.) as market for microalgal biomass for animal nutrition and a feed additive for cows. appearance. Chlorella and Scenesdesmus. • Aquaculture: Microalgae are also used as feed in the Benemann.. sold for the health food market. Pulz and Gross. Chlorella. Malcolm et al. market size for β–carotene is estimated to be greater than Pavlova. 600 t/yr Nostoc. Tetraselmis. animal feed are Spirulina.e. 1996). US$.g. 1996. The production includes ca. yet in the short term. 2006). 1990. currently at about 2. etc. Nannochloropsis. DHA by Crypthecodinium • Human Health Food Supplement: The consumption of and Schizochytrium. which may be added to human food and production at volumes and pricepoints that meet consumer animal feed for their health promoting properties demands or 2) new co-products that are low cost and have (Benemann. is quickly growing. is expected to grow in the future. Both the production plants and distribution channels) are for high. 1990...5 billion A number of anti-oxidants. Most as an extract or as a whole cell powder ranging in price frequently used species are Chaetoceros. Nitzschia. and is β–carotene from Dunaliella salina. Dunalliella. GLA from evening primrose oil). supplant the fossil fuels market) must be commodities • Animal Feed Additive: Microalgal biomass has also based to meet required volumes at price points acceptable been used with good results (i. arsenic. and ca.000 t/yr Chlorella. the biofuels markets will involve volumes even dogs and cats. better immune to the consumer. docohexaenoic acid (DHA). However. et al. and Thalassiosira. product. and eicosapentaenoic acid (EPA). currently at ~700 future algal-based biofuels market (designed in part to million US$... pigs. nascent algal-derived biofuels market: in the long term. The market size.. the DHA oil market is quickly growing. microalgal (of biomass. GLA by Arthrospira. which is sold either fish (Benemann. fertility. protein content and the level of unsaturated fatty value products or co-products from algae. In poultry rations. 2006). ca.10% (wt. e. 1994 and 1996). it is important to note that since the flue massive lipid production dominates. The Dunaliella.200 t/yr Dunaliella. 1. 1986. 3.5 billion US$.000 US$ per kg (Spolaore et al. 62 7. AA has been shown to be Food and Feed synthesized by Porphyridium. Nostoc and Aphanizomenon are more cost-effectively produced from non-algal sources (Radmer. Phaeodactylum and Nitzschia (Spolaore is currently restricted to only a few species. cadmium and derived biofuels. 1999). Yet the existing fossil fuels market is and the aquaculture feeds. thereby are known to be effective at metal absorption. With the possible exception of the existing response. is anticipated to continue until 1) a sufficient Polyunsaturated Fatty Acids (PUFAs) number of the challenges outlined earlier in the Roadmap for biofuel production have been overcome and associated Microalgae can also be cultured for their high content life cycle costs are reduced to realize sustainable biofuel in PUFAs. only DHA has been produced thus Spirulina (Arthospira). All other PUFAs to a lesser extent.) can be safely of commercial plants) that are significantly less than those used as a partial replacement for conventional proteins associated with the existing high-value algae-derived (Spoalore et al. However. The most prominent aquaculture of mollusks.) and scales (sizes and numbers biomass up to a level of 5 . the resulting non-oil algal improve the economic viability of some algae processes biomass is very likely to be unsuitable for use as an in the short-term. 2006). increasing fuel supplies without drastically increasing price. The main species used in products.

Phycobiliproteins. phycoerythrin and phycocyanin. have also been used in the feed of carp and even chicken (Puls and Microalgae-produced coloring agents are used as natural Gross. growth promoters.000 soil conditioners Metting&Pyne (1986) 7..500 Spolaore et al. 2006). has been successfully used as a salmon feed to cosmetics. and Exhibit 7. 1990). Benemann. pigments in pluvialis... are used as food dyes. cosmetics.. Spolarore et al.. 2006).2 Summary of microalgae commercial products market MARKET SIZE SALES VOLUME COMMERCIAL PRODUCT REFERENCE (TONS/YR) (MILLION $US/YR) BIOMASS Health Food 7. no current commercial market Spolaore et al. dyes for food. Pulz&Gross (2004) No available information 5. a carotenoid produced by Hematococcus Porphyridium. (2006) PUFA Extracts No available information 10 Pulz&Gross (2004) GLA Potential product. (2006) Animal Feed Additive No available information 300 Pulz&Gross (2004) POLY-UNSATURATED FATTY ACIDS (PUFAs) ARA No available information 20 Pulz&Gross (2004) Pulz&Gross (2004) DHA <300 1. 1986. 2003.500 Pulz&Gross (2004) Pulz&Gross (2004) Aquaculture 1. (2006) Phycocyanin No available information >10 Pulz&Gross (2004) Phycoerythrin No available information >2 Pulz&Gross (2004) FERTILIZERS/SOIL CONDITIONERS Fertilizers.. and research. Spolaore et al. Co-products   63 . (2006) ANTI-OXIDANTS Pulz&Gross (2004) Beta-Carotene 1. no current commercial market Spolaore et al. 2006). 2004. (2006) EPA Potential product.e.. or as pigments i..000 700 Spolaore et al.Coloring Agents the related carotenoids lutein and zeaxantin. (2006) Tocopherol CO2 Extract No available information 100-150 Pulz&Gross (2004) COLORING SUBSTANCES Pulz&Gross (2004) Astaxanthin < 300 (biomass) < 150 Spolaore et al. (Olaizola.200 >280 Spolaore et al.000 2. produced by in animal feed (Borowitzka. Astaxanthin.. and as fluorescent reagents in clinical or research give the fish meat a pink color preferred by the consumers laboratories (Spolaore et al. the cyanobacterium Arthrospira and the rhodophyte Astaxanthin..

3 Potential Options for the derived from microalgae (Metting and Pyne. plant growth regulators could be 7. the only issue is price. 1987). 2003. 1990.. Metting and Pyne. medical) $213 million Carrageenen (Food additive. pharmaceutical. Wu et al. Co-products   . 1986).. Radmer. 2001).3 Global value of seaweed products per annum (McHugh. etc.. 1990. wakame.e. The production bioactive compounds (Burja et al. Identical in functional performance to an existing chemical. Furthermore. is considered recalcitrant to ethanol fermentation since Currently. Exhibit 7. Pulz and Gross.. food additive. 2004). Recovery of Co-products Other Specialty Products Co-products from algal refineries should address one There are a number of specialty products and chemicals of these three criteria to be commercially viable and that can be obtained from microalgae. pharmaceutical. This occurs with natural oils which manufacturers in many cases would prefer if the costs Macroalgae possess high levels of structural were comparable. or with replacements such as algal polysaccharides that are extracted for their commercial proteins for distillers dried grains from corn dry grind value (Exhibit 7. pharmaceuticals and In this instance. Alginate. 1994.2 Commercial Products factor. mineral composition of depleted soils (Metting et al. but the source of the material can often provide from Macroalgae some advantage. but has been achieved for some chemicals and proteins/ 2004). the product can be labeled “organic” and thus saleable at a premium. and stable isotopes for material (shutdown economics). Olaizola. polysaccharides (Benemann. biological/microbiological) $132 million Alginate (Textile printing. seaweeds) are used as a plant the redox balance favors formation of pyruvate as the end fertilizer and to improve the water-binding capacity and product (Forro. 2001. 1990). Here price is a major 7. cosmetics (Spolaore et al. must be produced at a cost 30% lower than the existing 1986. Price becomes less of an issue if algae and agar and carrageenen from red algae. or other product. toothpaste) $240 million Other uses of seaweeds Fertilizers and conditioners $5 million Animal Feed $5 million Macroalgal Biofuels Negligible TOTAL $5. kombu. The market for these specialty products is likely to nutritional products. be very small due to their specialized applications. material it replaces and to be competitive typically. 1986). cost of the new product must be equivalent to the 1986.3).. fuel or other product. 1. Microalgal biomass could in principle serve the same purpose. fuel. Borowitzka. macroalgae (i. These include acceptable: bioflocculants (Borowitzka. Identical to an existing chemical. They include alginate from brown ethanol processing.5-6 BILLION 64 7.Fertilizers which occurs in high concentrations in brown seaweeds. pet food. 2005.) $5 billion Algal hydrocolloids Agar (Food ingredient. This sets a high bar research (Benemann.. 2003) PRODUCT VALUE Human Food (Nori. Singh et al. 2006). biopolymers and biodegradable plastics (Philip et al. 2. aonori. Pulz and Gross. Pulz and Gross.. it 2004). 2007.

and Benemann. could also be potentially considered for the recovery of energy from the lipid-extracted biomass Option 1.4 Overview of the five Algal Biomass potential options for the recovery and use of co-products Option 1 Extract Lipids Energy for Fuel Burn Residue Ash/ Soil Amendments Option 5 Soil Fertilizer/ Conditioners Dry Residue Processing Proteins as Option 2 Co-Products Food/ Feed Supplement Processing Option 3 Non-Fuel Surfactants/ Bioplastics Lipids Chemical/ Biological Conversion Option 4 Carbohydrates Ethanol/ Butanol/ Glycerol Chemical/ Biological Conversion 7.3. with Potential Use of a much larger fraction of biomass into fuels. These technologies are able to convert Lipid-Extracted Biomass. and combustion. as soil fertilizer and conditioner Each option and its associated technologies and future In addition to anaerobic digestion. The residuals • Option 4 – Recovery and utilization of carbohydrates remaining after anaerobic digestion could either be recycled from lipid-extracted biomass.pdf). 2009). conversion technologies. gasification.e. Recycle Nitrogen Gases Exhibit 7. which is significant in terms of reducing the overall cost of liquid biofuels production. with potential whole (i. The economic value of the produced extracted biomass for use in food and feed methane is equivalent to about $100 per ton of digested • Option 3 – Recovery and utilization of non-fuel lipids biomass. thermochemical research needs are discussed below. Nevertheless.unh. it may be difficult to identify large performance characteristics. Co-products   65 . such as pyrolysis. as is currently already done for • Option 5 – Recovery/extraction of fuel certain waste water treatment sludges (see http://www. lipids only. non-extracted) micro and macroalgal biomass use of residuals as soil amendments has been successfully demonstrated. the issues enough markets for potential co-products. In this case. New material with unique and useful functional production systems. Residuals as Soil Amendments these technologies are still in the testing and development Given the large amounts of lipid-extracted biomass residues stage. 2009). Therefore..4). and the glycerol as nutrients for algal cultivation or could be sold as soil from the transesterification of lipids to biodiesel fertilizers and conditioners. is the anaerobic • Option 1 – Maximum energy recovery from digestion of lipid-extracted biomass. the open market or used on-site in the various biorefinery operations. There are at least five different options for recovering economic value from the lipid-extracted microalgal biomass The most promising energy recovery technology. However. These are: from a practical and economic perspective. both (Exhibit 7. with reported methane yields of about 0. with use of the residual biomass edu/p2/biodiesel/pdf/algae_salton_sea.3 l per gram volatile solids (Huesemann • Option 2 – Recovery of protein from the lipid. could have poor or even negative energy balances (Huesemann and Benemann. and because of their large energy inputs (temperature that will likely be generated in future microalgal biofuels and pressure). Anaerobic digestion of the lipid extracted biomass. one are less related to costs and more to the functional option would be to convert as much of the lipid-extracted performance and potentially enhanced performance of biomass into energy. Maximum Energy Recovery from the (see Chapter 6). which could then be either sold on the new product.

Furthermore. (b) approximately 100 grams which is used in the formulation of a variety of industrial of cellulase are needed per gallon of ethanol. i. and could be advantageous.. polycarbonates and polyurethanes.5 billion gallons of lignocellulosic ethanol will be solvents. which contains proteins. 1995). this option (Datta et al. Recovery of Protein from the Lipid-Extracted It may be worthwhile. it may be possible to recover important industrial production of green solvents. to separate specific Biomass for Use in Food and Feed lipids present in the algal oil that have utility as chemical Following the extraction of lipids from the microalgal feedstocks for the manufacture of surfactants. supplementation with microalgal proteins to hydrogen. and (c) at products such as polymers. and butanol). it is also used in the In addition.the thermochemical conversion of lipid-extracted biomass Option 3.. Furthermore. solvents (acetone. Choi. ethanol. however. poultry. etc. might ethanol could be used as biofuel. acetic. Recovery and Utilization of Carbohydrates pets) is already very large and growing (estimated to rise from Lipid-Extracted Biomass. and as a renewable substitute (Zerlov et al. the protein fraction and specialty products such as urethanes. (Hu et al. relative derived ethylene and propylene. Assuming that (a) (Yazdani and Gonzalez. Similarly. aliphatic polyesters. and lactic) (Huesemann and Benemann. byproduct markets are likely to be overwhelmed. Lactic and byproduct prices will be greatly depressed versus acid. is the starting material for the production of polyester.txt). and the Glycerol from the to approximately 60 million tons per year for distillers dry Transesterification of Lipids to Biodiesel grains plus soluble (DDGS)) (Berger and Good. Furthermore. green solvents and a result. fish. Since biomass may contain sufficient levels of carbohydrates that protein is generally the key and often limiting ingredient could be converted through anaerobic dark fermentations in animal feed.150 per ton After the extraction of lipids. 1 billion kg. the market for animal feed (cattle. As of industrial surfactants. epoxies. the mineral-rich ash generated by these thermochemical and diesel substitutes will require specific ranges of carbon processes could possibly be used for nutrient recycle or as a chain length. butyric. thus butanol could serve to the amount of byproduct that would be produced. least 10.e. Option 2. Recovery and Utilization of Non-fuel Lipids has the potential advantage that the resulting nitrogen- It is well known that microalgae can synthesize a variety containing gases (e.. 2008). be recycled into the microalgal culture ponds. for chemical synthesis of a variety of products. thereby depending on the algal species and culturing conditions reducing the expense for nitrogen fertilizers. generate electricity directly in biofuel cells (Yildiz and 66 7. Hydrogen and The byproduct material. produced by 2020. ammonia and nitrous oxides) could of fatty acids with carbon numbers ranging from C10 to C24. antifreeze. biomass for liquid biofuel production. which can be converted into polypropylene oxide. adhesives. bioplastics. the projected market for cellulases is 2007. The Glycerol. Since the generation of gasoline. biodegradable plastics (Kamm and Kamm. As was pointed out above.. while butanol and organic make a useful animal feed. 2001). Furthermore.usda. pigs. Finally.3-propanediol. Co-products   . glycerol microalgal cellulases could be provided at a cost of less could be converted by certain bacteria to 1. Kawaguchi et al. the polymers that are currently produced from fossil oil- overall size of the animal feed market is small. than $0. from the residual biomass could be extracted and used as lubricants. 2007. 2007). 2009. due to issues such as taste of the meat or eggs. However. and its applications enzymes such as cellulases or other industrial enzymes include the pharmaceutical and agrochemical industries from the lipid-extracted biomass. it will be necessary to either separate the soil amendment. is potentially very large. a byproduct of the transesterification of market for industrial enzymes. human nutrition may organic acids (formic. However. feeding studies acids could serve as renewable feedstocks for the chemicals indicate that algae cannot be used as a high percentage industry. detergents. glycerol could be used to potentially very large. succinic. 2008). and paint (Yazdani and Gonzalez.20 per gallon ethanol. and Option 4. product into the appropriate range or rearrange the carbon chains through catalytic cracking and catalytic reforming. 2007). a food and feed supplement. including and interactions with animal specifically cellulases for microalgal lipids to biodiesel. The current price for DDGS ranges from $110 . For example. current levels. the individual local markets for animal feed are often not succinate is an intermediate in the production of a variety located adjacent to areas where algae may be produced. butanol is a valuable C4 compound of feed.. 2007). 2006). the residual microalgal (http://www. could also be anaerobically pretreating lignocellulosic feedstocks prior to fermentation fermented to the above mentioned and other end products to fuel ethanol. would require the use of specially selected or engineered microalgal strains capable of producing these enzymes.. jet fuel. also benefit from supplementation with microalgal proteins.g. propionic.ams. Kamm and Kamm.

(2008). these are the development of high throughput technologies for the quantitative characterization of References Benemann. Forro. polysaccharides for use as emulsifiers in food and industrial non-fuel lipids.000 tons of glycerol (http:// tools to improve yields of desired products. it applications (Mooibroek et al. Kamm. and the development of genetic engineering the formation of more than 400. Biologically active compounds from microalgae. J. Glycerol-based biorefinery for fuels and chemicals. including www. Developments in industrial microbiology. 9347-9377.S. P. Agronomic uses of seaweed and microalgae. I.J. Tsai. (8). and carbohydrates is not cost-effective. Metting. additional capacity from algal lipids may find it Use of the Residual Biomass as Soil Fertilizer exceedingly difficult to find uses. Recent Patents on Biotechnology. Microalgal triacylglycerols as feedstocks for biofuel production: Perspectives and advances. Hu. with saturated. Good. 5.. Food and Agriculture Organization. D. Recovery (Extraction) of Fuel Lipids Only.L. Kawaguchi H. and biotechnology.. Journal of Biosciences Bioengineering. A Report from the Department of Agricultural and Consumer Economics.. Science Publishers. Co-products   67 . Datta. (16). (2007). Frank. 2007). (1987). 3(12). Zimmerman. A. (2001). Distillers dried grains plus solubles utilization by livestock and poultry in corn-based ethanol in Illinois and the U.Kadirgan. M.biodieselmagazine.R. Biofuels from microalgae: Review of products. including sugars and complex Production of 1 billion gallons of biodiesel will result in carbohydrates. A. W. (91). Jarvis. (2).. The alga dunaliella: biodiversity. B.. Technological and economic potential of poly(lactic acid) and lactic acid derivatives.R. Crouch. FEMS Microbiological Review.. Moon. Pyne.A. Q. Developments in Aquaculture and Fisheries Science (Netherlands). M. R&D efforts as discussed under Chapter 2. (105). Wright. this option will also require and potentially growing. (2001). Banaigs.H. Benemann. Tetrahedon. 621-63. J. organic matter as soil fertilizer and conditioners.e. (31). van Staden. H2 production from algal biomass by a mixed culture of rhodobium marinum A-501 and lactobacillus amylovorus. Algal Biology. Hashimoto. Miyamoto.G.R. A guide to the seaweed industry. No. microalgal metabolites. (2007). M. J. (54). M. the recovery and use of energy. M. Burgess. J. Huesemann. M.175-204. (2009). and Conditioner In case none of the above mentioned four options are It may also be possible to extract microalgal economical.M. Choi. the market for organic fertilizer is large As was the case with Option 3. L. Microbiological Sciences.jsp?article_id=377). As was mentioned above. K. New Hampshire. Abou-Mansour. S. with special focus on Dunaliella sp. (1986). 372-375. Champaign-Urbana. Seibert.C. carbohydrates. As the current production levels for biodiesel (700 million gallons in 2008) already has the market for glycerol Option 5. The Hague. 277-282. proteins.H. Ghirardi. (1990). specifically. physiology. Enzyme Microbial Technology. Kamm. 173-180. 247-256. Hirata. carbohydrates could be recycled into pulp and paper which involves the extraction of only fuel lipids and the streams. Berger. B. processes. (2006). J. 386-394. B. Once again. i. Microbial degradation of marine biomass. Introduction to Applied Phycology. and P. 7. 1994). Posewitz. E. K. Marine cyanobacteria – A prolific source of natural products. M. The Plant Journal. Microalgae as sources of fine chemicals. Biorefineries – multi product processes. R. (57). Suppl..W. (1990). Bonsignore. Netherlands.. the issue of scale enters in. Borowitzka.. (1986). Metting. Advances in Biochemical Engineering/Biotechnology. S.P. L. substituting for lignocellulosic materials derived subsequent use of the biomass residues rich in nitrogen and from forestry resources. University of Illinois. microalgal is possible to revert to the most simple option (Option 5). W.J. J. Sommerfeld. Journal of Industrial Microbiology. McHugh. 589-627. J. Burja. Darzins. if desired. J. genomics. 221-231. B. SPB Academic Publishing. E. K. Finally. and potential. (2008). (2003). Microalgae products and production: An overview.

R. 635-648. 85-90. 723-730. Current Opinion Biotechnology. W. Applied Microbiology and Biotechnology.Mooibroek. (2007). Zerlov. C.J. (76). Bacterial acetone and butanol production by industrial fermentation in the Soviet Union: Use of hydrolyzed agricultural waste for biorefinery.C.. Journal of Electrochemical Society. (2006). (6). P. M.. Willke. Journal of Bioscience and Bioengineering. Algal diversity and commercial algal products. Parker. Toonen. Shen.F. Accumulation of poly-b-hydroxybutyrate in cyanobacterium synechocystis sp. Giuseppin. (1996).A. Roy. V. Sanders.. Yildiz. (2007). Olaizola.V. Co-products   .J. Berezina. Scott. Wu. Journal of Applied Phycology. Journal of Chemical Technology and Biotechnology. T. (1994).Valuable products from biotechnology of microalgae. Wu.Y.H. Bioactive compounds from cyanobacteria and microalgae: An overview. 87-96. Philip.Y. Steinbüchel. (2004). G. Yazdani. Banerjee. 101(2). Radmer. Gonzalez. (65).. J. E. S. Commercial applications of algae: Opportunities and Constraints. (2006). 459-466..D. A. S. 213-219.. 257-267. Kate. Industrial bioconversion of renewable resources as an alternative to conventional chemistry. Kadirgan. B. Schwarz. Pulz. (2001). (71).H. 233-247. 68 7. H. A. B. 263-270.. (20). I. F. Electrocatalytic oxidation of glycerol. Applied Microbiology and Biotechnology. Singh. Applied Microbiology and Biotechnology. (2005). Q. W.. PCC6803. Joannis-Cassan.. Polyhydroxyalkanoates: Biodegradable polymers with a range of applications. 46(4). Assessment of technological options and economical feasibility for cyanophycin biopolymer and high-value amino acid production. (2003). 131-142. M. Isambert. S. M.C. (18). Velikodvorskaya. Franssen. Radmer. Gross. 73-95. Vorlop. R.N. N. (2007).. R. (77). Duran. (1994). U. (141). H. Applied Microbiology Biotechnology. (2004). G. E. Spolaore. Bioresource Technology. K. Keshavarz. O. G. 93-98. O. Biomolecular Engineering. Critical Reviews in Biotechnology. BioScience.S. (66). Anaerobic fermentation of glycerol: A path to economic liability for the biofuels industry. Commercial applications of microalgae. (82). (25). 587-597. T. Z.. Oosterhuis. Commercial development of microalgal biotechnology: From the test tube to the marketplace.

and completely compatible with the existing liquid transportation fuel infrastructure. fuels intermediates. Although the biofuel product(s) from algae will not require significant distribution system from algal biomass would ideally be energy-dense modifications during or after processing in the refinery. which carries infrastructure costs can be lowered in four ways: implications for the producers of algae and selection • Minimizing transport distance between process units. blending. 1990). container dimensions.1 Distribution of algal lipid intermediates have not yet been established. • Maximizing compatibility with existing infrastructure 1995). have been shown biofuels as well. biodiesel. and transport scenarios is these logistics through end-use issues influence siting. Some of these fuels are to be sited. 2009). Thus... the stability of the algal biomass under different decision points. high capacity. and the ultimate economics and operations of that natural bacterial communities increase the an integrated algal biofuels refinery. 8. It is conceivable that these “bio-crudes” Lowering costs associated with the delivery of raw will be compatible with current pipeline and tanker biomass. it is known that the presence feedstock production center to the ultimate consumer of unsaturated fatty acids causes auto-oxidation of are common goals for all biofuels.g. depending on whether it will be dewatered/ • Optimizing the scale of operations to the parameters densified biomass and/or fuel intermediates that are to stated above. diesel. jet-fuel blends from a variety can be expected to influence the economics of algal of oil-rich feedstocks. transporting. Each of these different fuels has where the algae production and biorefinery facilities different implications for distributions. products – ethanol. fuel intermediates. and bioproducts. poorly characterized. fuel intermediates. combusting. conforming to existing standards (e. Ethanol. and end-use vehicles). In turn. dispensing equipment. and hydroreformed biofuels – are being considered harvesting conditions differing in salinity. In all cases. be transported to the refinery. Specifically. These have been challenging issues appear to be more compatible with the existing petroleum for lignocellulosic feedstocks (Hess et al. and different fuels from algae are being considered. implications for biodiesel stability (Bondioli et al. storage. As a variety of fuel rate of algae decomposition (Rieper-Kirchner.. biofuels. similar to plant lipids may be possibly taken into pipelines. Distribution and Utilization Distribution and utilization are challenges associated with It is also anticipated that gasoline and diesel range fuels virtually all biofuels. pyrolysis oil. 1986). However. 2009) and infrastructure. Efstathiou and Credeur. it is difficult to predict how utilization challenges associated with each of these possible these factors will influence biomass storage and opportunities is discussed. 2008). hazardous Distribution is complicated by the fact that several materials and associated human health impacts. the specific distribution and dewatering levels. biofuels oils (Miyashita and Takagi. to be compatible for use in select demonstration flights (Buckman and Backs. few studies exist that While the demonstration flights mitigate some address outstanding issues of storing. account for the storage of algae lipids (Hu et al.. and jet fuels are all possible products for energy-inputs and costs. such as cultivation and harvesting. materials and temperature considerations (e. In the context of a variety of culturing and syngas. for ideal lipid compositions. other distribution aspects pipelining. and the quality of the final fuel product. as corrosivity) for trucks. production. Distribution and Utilization  69 . pH and from algal biomass resources. renewable transport method(s) should be analyzed and optimized gasoline. including algae. biodiesel. biogas. with some evidence suggesting scalability. • Second. infrastructure concerns. distribution and utilization need to be discussed in the context of earlier • First. higher alcohols. remain poorly studied: Being intermediate steps in the supply chain. The optimal Technologies). and final fuels biomass. an issue that impacts oleaginous microalgae feedstocks is that the transport and storage mechanisms 8. and final fuels from the systems. within the context of from algal biomass. It is also known that temperature and storage material have important • Maximizing the substrate energy-density and stability. transport. rails. storage tanks. and • Third. delivery vehicles. and dispensing algal concerning algal biomass. and barges is critical discussed in detail in Chapter 6 (Algal Biofuel Conversion to minimizing infrastructure impacts.g.8. 2009.

Ethanol is another likely fuel from algae. With over Typically, compliance with specifications promulgated by
10 billion gallons per year produced and consumed organizations such as ASTM International ensures that a
domestically, distribution-related issues for ethanol has fuel is fit for purpose (ASTM International, 2009a, 2009b,
been studied for some time, and algal ethanol can benefit 2009c, 2009d, and 2009e). Failure of a fuel to comply with
from these analyses. While not as energy dense as purely even one of the many allowable property ranges within
petroleum-derived fuels, ethanol is an important fuel the prevailing specifications can lead to severe problems
oxygenate that can be used in regular passenger vehicles in the field. Some notable examples included: elastomer-
and special flex-fuel vehicles at up to 10% and 85% gasohol compatibility issues that led to fuel-system leaks when
blends, respectively. However, considerable infrastructure blending of ethanol with gasoline was initiated; cold-
investments need to be made for higher ethanol blends weather performance problems that crippled fleets when
to become even more attractive and widespread. One blending biodiesel with diesel was initiated in Minnesota
issue is that ethanol is not considered a fungible fuel; it in the winter; and prohibiting or limiting the use of the
can pick up excessive water associated with petroleum oxygenated gasoline additive MTBE in 25 states because
products in the pipeline and during storage, which causes it has contaminated drinking-water supplies (McCarthy
a phase separation when blended with gasoline (Wakeley and Tiemann, 2000). In addition to meeting fuel standard
et al., 2008). One possible way to address this is to build specifications, algal biofuels, as with all transportation
dedicated ethanol pipelines; however, at an estimated fuels, must meet Environmental Protection Agency
cost of $1 million/mile of pipeline, this approach is not regulations on combustion engine emissions.
generally considered to be economically viable (Reyold,
2000). Another possibility is to distribute ethanol blends by As is true of any new fuel, it is unlikely that new
rail, barge, and/or trucks. Trucking is currently the primary specifications will be developed for algal fuels in the near
mode to transport ethanol blends at an estimated rate of term (i.e., at least not until significant market penetration
$0.15/ton/kilometer (Morrow et al., 2006). This amount is has occurred); hence, producers of algal fuels should
a static number for low levels of ethanol in the blends (5% strive to meet prevailing petroleum-fuel specifications.
to 15%); as the ethanol content in the blend increases, the Nevertheless, research and technology advancements
transport costs will also increase due to the lower energy may one day yield optimized conversion processes which
density of the fuel. can deliver algae-derived compounds with improved
performance, handling, and environmental characteristics
8.2 Utilization relative to their petroleum-derived hydrocarbon
counterparts. If significant benefits can be demonstrated,
new specifications can be developed (e.g., ASTM D6751
The last remaining hurdle to creating a marketable new
and D7467).
fuel after it has been successfully delivered to the refueling
location is that the fuel must meet regulatory and customer
requirements. As mentioned in Chapter 6, such a fuel is The discussion below is divided into separate sections that
said to be “fit for purpose.” Many physical and chemical deal with algal blendstocks to replace gasoline-boiling-
properties are important in determining whether a fuel is fit range and middle-distillate-range petroleum products,
for purpose; some of these are energy density, oxidative and respectively. These classifications were selected because
biological stability, lubricity, cold-weather performance, the compounds comprising them are largely distinct and
elastomer compatibility, corrosivity, emissions (regulated non-overlapping. Within each of these classifications,
and unregulated), viscosity, distillation curve, ignition hydrocarbon compounds and oxygenated compounds are
quality, flash point, low-temperature heat release, metal treated separately, since their production processes and in-
content, odor/taste thresholds, water tolerance, specific use characteristics are generally different.
heat, latent heat, toxicity, environmental fate, and sulfur
and phosphorus content. Petroleum refiners have shown Algal Blendstocks to Replace
remarkable flexibility in producing fit-for-purpose fuels
Middle-Distillate Petroleum Products
from feedstocks ranging from light crude to heavy crude,
oil shales, tar sands, gasified coal, and chicken fat, and are Petroleum “middle distillates” are typically used to create
thus key stakeholders in reducing the uncertainty about the diesel and jet fuels. The primary algae-derived blendstocks
suitability of algal lipids and carbohydrates as a feedstock that are suitable for use in this product range are biodiesel
for fuel production. (oxygenated molecules) and renewable diesel (hydrocarbon
molecules). The known and anticipated end-use problem
areas for each are briefly surveyed below.

70 8. Distribution and Utilization 

Oxygenates: Biodiesel isomerized (i.e., transformed from straight- to branched-
chain paraffins), renewable diesel and SPK will have
Biodiesel is defined as “mono-alkyl esters of long
cold-weather performance problems comparable to those
chain fatty acids derived from vegetable oils or animal
experienced with biodiesel. Also, as was the case with algal
fats” (ASTM International, 2009b). Biodiesel has been
biodiesel, contaminants and end-product variability are
demonstrated to be a viable fuel for compression-ignition
engines, both when used as a blend with petroleum-derived
diesel and when used in its neat form (i.e., 100% esters)
(Graboski and McCormick, 1998). The primary end-use Algal Blendstocks for Alcohol and
issues for plant-derived biodiesel are: lower oxidative Gasoline-Range Petroleum Products
stability than petroleum diesel, higher emissions of nitrogen
oxides (NOx), and cold-weather performance problems While much of the attention paid to algae is focused on
(Knothe, 2007). The oxidative-stability and cold-weather producing lipids and the subsequent conversion of the lipids
performance issues of biodiesel preclude it from use as a to diesel-range blending components (discussed above),
jet fuel. The anticipated issues with algae-derived biodiesel algae are already capable of producing alcohol (ethanol)
are similar, with added potential difficulties including: directly, and there are several other potential gasoline-range
1) contamination of the esters with chlorophyll, metals, products that could be produced by algae-based technology/
toxins, or catalyst poisons (e.g., sulfur and phosphorus) biorefineries. Petroleum products in the alcohols and
from the algal biomass and/or growth medium; 2) undesired gasoline range provide the major volume of fuels used by
performance effects due to different chemical compositions; transportation vehicles and small combustion engines in
and 3) end-product variability. the United States. Ethanol or butanol are the most common
biofuels currently being considered for use in gasoline,
and these alcohols can be produced from fermentation of
Hydrocarbons: Renewable Diesel and starches and other carbohydrates contained in algae.
Synthetic Paraffinic Kerosene
The hydrocarbon analog to biodiesel is renewable diesel,
which is a non-oxygenated, paraffinic fuel produced Additionally, the hydro-treating of bio-derived fats or
by hydrotreating bio-derived fats or oils in a refinery oils in a refinery will typically yield a modest amount of
(Aatola et al., 2009). Algal lipids can be used to produce gasoline-boiling-range hydrocarbon molecules. Refiners
renewable diesel or synthetic paraffinic kerosene (SPK), refer to this material as “hydro-cracked naphtha.” This
a blendstock for jet fuel. These blendstocks do not have naphtha tends to have a very low blending octane and
oxidative-stability problems as severe as those of biodiesel, would normally be “reformed” in a catalytic reformer
and renewable diesel actually tends to decrease engine- within the refinery to increase its blending octane value
out NOx emissions. Nevertheless, unless they are heavily prior to use in a gasoline blend.

8. Distribution and Utilization  71

Aatola, H., Larmi, M., Sarjovaara, T., and Mikkonen, S. (2009). Hydrotreated vegetable oil (HVO) as a renewable diesel fuel: Trade-off
between NOx particulate emission and fuel consumption of a heavy duty engine. SAE International Journal of Engines. 1(1), 1251-
ASTM International. (2009a). Standard specification for diesel fuel oils. West Conshohocken, PA: ASTM International. Available online
ASTM International. (2009b). Standard specification for biodiesel fuel blend stock (B100) for middle distillate fuels. West Conshohocken,
PA: ASTM International. Available online at
ASTM International. (2009c). Standard specification for aviation turbine fuels. West Conshohocken, PA: ASTM International. Available
online at
ASTM International. (2009d). Standard Specification for Automotive Spark-Ignition Engine Fuel. West Conshohocken, PA: ASTM
International. Available online at
ASTM International. (2009e). Standard specification for diesel fuel oil, biodiesel blend (B6 to B20). West Conshohocken, PA: ASTM
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72 8. Distribution and Utilization 

Critical requirements—such as suitable land and climate. as with any biomass-based technology and industry. sourcing of nutrients from both the input wastewater Additional resources include materials. land-based photoautotrophic microalgal terms of their geo-location. CO2. treatment and necessary technical improvements for co-producing algal biofuels are described.S. The status of algae-based wastewater and conducting operations and maintenance. the major resource and environmental parameters that Further. nutrients. Resources and Siting   73 . off-shore marine environment CO2 sources with algae production are outlined. For each parameter. capital. The sustainability and environmental impacts biofuel could be supported if sufficiently high algae of national algae production capacity build-up and productivities could be achieved affordably at scale.. In addition to coastal and inland photoautotrophic the challenges associated with coupling industrial microalgae production. a for algal biofuel production. as Different Cultivation Approaches well as from a resource availability and use perspective. Maxwell et al. transportation fuel needs and displacing petroleum use. required Photoautotrophic microalgae production energy inputs. This chapter provides an overview of the issues associated with site location and key resources for various microalgae Exhibit 9. availability. other nutrients. needs to be analyzed from a site location. water. Lansford et al. To achieve success regarding both Program (Sheehan et al.. and land Sourcing of suitable organic carbon feedstock. and conditions of the particular algae species being cultivated CO2 resources were identified at the time to suggest and the engineered growth systems being developed and that the production of billions of gallons of algal deployed. microalgae to significantly contribute to meeting U. both of which influence siting decisions production efficiency. Resources and Siting The development and scale-up of algal biofuels 9. energy.. These parameters with wastewater treatment and industrial sources of are of greatest importance to siting. Sufficient land. the siting and resource factors (e. and costs. an in-depth discussion is included on the potential pertain to the algae biofuels production inputs of climate. focusing primarily on technical and economic performance without adverse the Southwest and southern tier of the United States environmental impacts. operation over time will be important complementary Many of the findings of these earlier assessments still aspects of the siting and resources issues that will also apply today and the potential remains for biofuels need careful consideration and analysis using tools and other co-products derived from photoautotrophic and methodologies discussed later in chapter 10.. supplemental CO2 supply.1). This scenario Exhibit 9.1 Key resource issues for different algae systems ALGAE PRODUCTION APPROACH KEY RESOURCE REQUIREMENTS Climate. labor. cultivation were conducted during the Aquatic Species and affordability. concentrated CO2. CO2. facilities design.g.1 Resource Requirements for production. must also be appropriately matched to the required growth Feinberg et al. to couple land-based microalgae biomass production water. 1990. and land. water. Assessments of resource requirements and availability and other nutrients—must be appropriately aligned in for large-scale.. characteristics. 1990). concepts are also being proposed.9. energy. Photoautotrophic Microalgae Approach sustainable water resources. Integration with wastewater variety of conditions may be more or less cost-effective treatment can play an additional important role in the for the siting and operation of algal biomass production. and from possible nutrient recycling from residual and other inputs associated with facilities infrastructure algal biomass. and other inputs required for siting and operating industrial Heterotrophic microalgae production bioreactor-based algae production and post-processing to fuels and other co-products Availability of suitable coastal and off-shore marine site loca- Photoautotrophic macroalgae production tions 9.2 provides a simple high-level overview of and macroalgae production approaches (Exhibit 9. Similarly. water. 1985. 1998).

whereas photoautotrophic Photoautotrophic Macroalgae Approach production. Use of sugars from cane.g. selected to meet production needs. and lignocellulosic conceptually include the equivalence of land and biomass. The Resource issues for the heterotrophic approach are more merits of each should be carefully evaluated. beets. Achieving affordable scale-up and has the greatest feedstock scale-up potential and is being successful commercial expansion using the heterotrophic pursued and reported elsewhere through bioenergy approach relies on the cost-effective availability of organic programs under DOE and Department of Agriculture carbon feedstock—a resource that ultimately links back to (USDA. that is expected to be capable of national scale-up to Heterotrophic production can be characterized as more of over one billion tons annually (Perlack et al. DOE. Climate CO2 Nutrients Nutrient Recycle Liquid and Gasous Fuels Processing Algal Harvesting and Production Conversion Secondary Products Water Land Climate Water Land Resources and Insolation Location Topograghy Enviromental Temperature Supply/ Demand Use/ Cover Exhibit 9. and land-based ponds. This a photosynthetic origin (Exhibit 9. thereby sharing many of the same feedstock culture facilities with off-shore areas and structures. an industrial operation with a significant upstream logistics trail associated with the sourcing of the needed biomass- derived input feedstocks. The algae are adverse food/feed market impacts would be based on cultivated in the dark in closed industrial bioreactors the use of carbon-rich waste streams and the successful that could potentially be established in many locations deconstruction of lignocellulosic materials. Heterotrophic and includes siting and resource issues that are closely photoautotrophic approaches to microalgae production coupled with the production.and second-generation biofuels. similar conceptual extension holds for the application of Exhibit 9. The preferred source of sugars production is based on the use of organic carbon feedstock and other appropriate organic carbon feedstocks for in the form of sugars or other relatively simple organic greatest sustainable scale-up potential and avoidance of compounds instead of photosynthesis. lead to the Heterotrophic Microalgae Approach problem of linking biofuel production with competing Heterotrophic microalgae biomass and metabolite food and feed markets. have different siting and resource input implications and pretreatment of lignocellulosic biomass feedstock and thus present synergistic integration opportunities.. The latter throughout the country..2 to off-shore macroalgae production. cost and availability of space 74 9.2 to organic carbon-rich waste streams. in terms of cultivation and harvesting. 2005). is more Options for siting macroalgae (also known as seaweed akin to agriculture and serves as the point of origin for the or kelp) biomass production include offshore farms. 1985) can be represented by extension of Exhibit 9. 2006a). near-shore coastal farms. availability. supply logistics. biomass feedstock supply for the downstream value chain. other sugar crops. e. Perlack et al. Resources and Siting   . A supply issues with first.2 Key siting and resource Parameters Evaporation Salinity Ownership elements for algae biofuel production Severe Storms Allocation Soils Geology (Adapted from Maxwell et al. taking into largely associated with the upstream supply of organic consideration factors such as the scale of farms required carbon feedstock derived from commodity crops. and from the hydrolysis of starch grain crops can.3). after sufficient scale-up of production and demand.. 2005..

2004) factors include solar ponds CO2 . P. weather conditions. particularly in the case of land-based with other uses. are less sensitive to climate variability than open ponds Based on the scale of macroalgae cultivation practices due to the more controlled environment that closed currently being used to meet non-fuel product demand systems can provide. and favorable regulatory Heterotrophic algal cultures can be one to two orders of framework for coastal and offshore environment use could magnitude higher in biomass density than possible with help enable cultivation at scales to meet production goals. Heterotrophic algae production A major challenge lies in finding and developing new in closed industrial bioreactor systems utilize mature environments and cultivation approaches that will support bioreactor technology that can provide relatively clean production of macroalgae at the larger scales and lower and protected environmental control needed to optimize costs needed to supply the biofuels market.power plants . has also been proposed as a way of leveraging other evaporation. As illustrated in Exhibit 9. Ligno-Cellulosic N. temperature. precipitation. rivers Biomass Harvesting or Other Non-Fresh Waters Lignin Dewatering Extraction & CHP Seperation High-Density Algal Biomass FHarvesting Exhibit 9. K. the level of production affect productivity. other Water Treatment Deconstruction . From a resource use perspective. whereas precipitation.4 million dry sunlight.lake. research. This is done at the expense of Various climate elements affect photoautotrophic algae 9. will most directly metric tonnes for food products). Synergistic Project Intergration Opportunities Ligno-Cellulosic Autotrophic CO2 Sourceess Plant Material algae production .2 Resources Overview reduce both water and energy-use intensity in the algae production operation. and severe weather. Additional growth conditions for higher biomass density CAFO Low-Density Algal . Closed photobioreactors technologies to facilitate the cultivation of macroalgae. key other structures such as windfarms (Buck et al. and severe weather will affect water supply and water quality in open systems. and competition production. Temperature and availability of (annual global production of about 1.hybrid mix Nutrient-Rich Water. The co-siting of macroalgal farms with microalgae production. Resources and Siting   75 .3 High-level Oil Feedstock illustration of heterotrophic Biomass Residue and photoautotrophic Sugars and Other Heterotropic algae approaches to microalgal Organic Carbon production in Fuels biomass and biofuels Feedstock closed bioreactors production Co-Products and nutrients. evaporation. would need to be greatly expanded for biofuels.other industrial . and day/night light and temperature cycles.. both seasonally and annually.closed PBRs .muni WWT & Seperation . technology development. environmental impacts. Closed heterotrophic bioreactor Climate systems are decoupled from the variable ambient Sunlight and Temperature Needs outdoor climate.4. open photoautotrophic systems that typically achieve no more than about 1 gram per liter culture density. the heterotrophic approach can 9.

having gross qualitative similarity to Exhibit 9. annual average daily temperature offshore marine macroalgae production. currents. Wisconsin. from the PNNL study show clear patterns relating climate Texas. A significant portion Laboratory (PNNL) using higher GIS resolution and of the United States is suitable for algae production more information-rich climate and meteorological data. and enclosures requiring geographical regions were: annual average cumulative energy input for environmental control. The more to total biomass growth. This could fresh water. The climate criteria used to narrow down the production at higher latitudes might be possible with Exhibit 9.4 presents some simple GIS-based scoping solar insolation. from the standpoint of having adequate solar radiation Projections of annual average algae biomass production (with parts of Hawaii. and Florida being most promising). lower elevation sites.high-cost systems. with the major climate element affecting algal It was recognized from the outset that the correlation growth being the availability of sunlight. and annual average freeze-free days ≥ 200.4 Rough scoping assessment of preferred site locations for outdoor algae production a) Regions with annual average climate conditions meeting b) Fossil-fired power plant sources of CO2 within 20 miles of selected criteria: ≥ 2800 hour annual sunshine. California. and ≥ 200 freeze-free days region 76 9.. Resources and Siting   . In Exhibit 9. controls. This is likely due to Preferred Geographic Regions for Algae Production more moderate annual average temperatures despite lower Exhibit 9. but suggest was to provide a preliminary high-level assessment preference for lower latitude. identifying preferred regions of the United States for photoautotrophic microalgae production based on Additional factors could conceivably overcome what the application of selected filter criteria on annual might otherwise appear to be uneconomical site and average climate conditions.4 algae is technically feasible in many parts of the United (a). New Mexico. with the higher growth regions northern latitude states. Growth of based on the modeling assumptions used.4 and the Michigan. would have southern tier states showing greatest productivity potential very low productivity in the winter months. For the case of sun hours ≥ 2800. Arizona. Storm and ocean of these annual average climate criteria with what dynamics affecting waves. the lack of attractiveness of the Gulf Coast region States.e. productivity in large-scale deployed systems could only be taken as a gross indicator. Within that context. the ocean or sea ≥ 55°F. resource conditions for algae production. and marine nutrient might actually be achieved with annual average algae transport cause additional impacts on productivity. These results should be considered simply conducted by Sandia National Laboratories. whereas PNNL study suggests projected productivities in this region could be relatively high. the results are suggestive and consistent with with both open and closed cultivation systems is the the more recent assessment by Pacific Northwest National availability of abundant sunlight. i. including Minnesota. the availability of non. environment provides a buffer that moderates temperature variation. and the availability of concentrated sources include situations where co-location of inland microalgae of CO2. resource factors that will determine economic feasibility. and the New England states. but the availability of adequate sunlight and the from southeast Texas to northwest Florida is attributed suitability of climate and temperature are key siting and to the lower annual average solar insolation available. annual municipal wastewater facilities in the preferred climate average temperature ≥ 55˚ F. Equally important for photoautotrophic microalgae growth however. The goal indicative at this point rather than predictive.

Such analyses should include assessment of the monthly or Water Requirements seasonal solar radiation and ambient temperature ranges. integrated with co-located industries providing synergistic particularly extremes in temperature. and currents that can cause the lower elevation regions of the lower latitude states disruption or damage to physical structures and operations. both high and low. For closed photobioreactors. but also with analysis for systems that would likely be closed and highly algae that grow well under severe climate conditions. Evaporation is a Therefore. and hurricanes pose serious concerns in the inland regions of the central states. Thorough analysis (preferably on a state-by-state basis) with detailed data is needed to assess areas most suitable Water for algae production based on this climate factor. Georgia. implications for energy and cost needed to maintain internal temperature sustainable production scale-up. cooling in the summer. Southeast. These weather length of growing season are latitude and elevation. locations for open pond production. although evaporative cooling is often viable growing season(s) for the particular strains of algae considered as a means to reduce culture temperature. of available sunlight per day and the daily and seasonal and need to be taken into account when looking at temperature variations. Arizona. the Northwest. This would. and New the conventional crop analogy for this is the length of time Mexico) and Hawaii have the highest evaporation rates between the last killing frost in the spring and the first in the United States. different algal species capable of good productivity in cold season and hot season conditions. is a wide range of temperatures. 1985). such as heavy rain and flooding. tornadoes. Offshore marine algae insolation and average daily temperatures above the lower production will be subject to severe weather impacts threshold needed for economically viable growth) are in the form of wind. however. Seasonal Considerations are desirable for algae production from the standpoint Various species of microalgae have potential for biofuel of long-term availability and sustainability of water feedstock production and are capable of growing under supply. discussed later in this section. Florida. throughout the seasons. Discovery and development of algae species capable of increased General Water Balance and Management Needs productivity under wider ranges of light and temperature One of the major benefits of growing algae is that. Other local to materials and usually demands both the use of higher climate and weather conditions will also have influence. represented by specific regions of Hawaii. of Hawaii. unlike conditions can also potentially lead to increased annual most terrestrial agriculture. waves. require a more refined biomass productivity and oil content yield. precipitation (more than 40 inches). Evaporative however. and parts of Louisiana. Evaporation. respectively. would Severe Weather Events and Elements provide greater flexibility and could extend otherwise Severe weather events. Resources and Siting   77 . Availability and rotation of Evaporative water loss is discussed in a later subsection. will require that suitable climatic conditions loss can be a critical factor to consider when choosing exist for a major part of the year (Maxwell et al. Arizona. dust storms. for closed reactors. and the Southeast. quality and more costly materials and greater maintenance.industrial operations capable of providing excess heat and locations. The southwestern states (California. It is encouraging that researchers today power for cost-effective environmental control of the algae are not only concerned with finding algae with high cultivation. a critical climate issue for both open and closed less important concern for selecting locations of closed photobioreactor systems is the length of economically photobioreactors. and California. hail storms. Precipitation affects water availability (both surface it should also establish minimum economically feasible and groundwater) at a given location within a given operational requirement values for heating in the winter watershed region. Areas with higher annual average and. limited periods of commercially viable algae production. High annual production closely coupled with climate and will increase water for a given species grown photoautotrophically outdoors. For outdoor ponds. and overall economics. The marine environment can also be highly corrosive Texas. events can contaminate an open system environment or which have major influence on the hours and intensity cause physical damage to both open and closed systems. and coastal areas. Areas with relatively long growing prospects for algae production in both inland and coastal seasons (for example. killing frost in the fall. algal culture can potentially average productivities in more geographically diverse utilize non-fresh water sources having few competing 9. The primary geographical location factors for determining Southwest. with more than 60 inches annually. requirements for an open algae growth system. 240 days or more of adequate solar regions of the United States. the A thorough evaluation of evaporative water loss is conventional crop analog is the greenhouse and the limiting needed to assess water requirements.. opportunities for utilizing waste heat and energy. available for productive cultivation. New Mexico.

were for thermoelectric-power generation. 2008. Hightower et al.. water-sparse locations. can also become an environmental problem for inland where a growing number of counties are experiencing locations. such as saline and brackish ground water. ). 2002). from both a resource perspective could have major effect on all sectors of water resources and an algal cultivation perspective. A rough upper bound estimate of evaporative water loss 78 9. hybrid combination). or “co. At the national scale.. 1999. following the more than doubling of methane wells (Reynolds. Many of the nations’ fresh ground water aquifers are under up. population increase has thus far been offset by increased down of ponds after salinity build-up. 2006b. the thermoelectric-power industry in the coastal states whether evaporative cooling is used for closed systems. or from desalination operations. Mgal/d and were the second-largest category of water and wind conditions (Al-Shammiri. nearly and the site-specific details of climate. wind. for irrigation of crops and other lands totaled 128. Climate change is also recognized as a factor that management issues.. Saline water (seawater and heterotrophic assessment. Mao. the application of desalination treatment to control increasing stress. Kenny et al. humidity. However. 2003. Growing demand for limited will change with evaporative water loss. Water balance and supply from precipitation (DOE.000 ambient temperature variations. is used by microalgae (open vs.. This will require periodic blow. Kenny et al. Water Supply and Management Water use and consumption for algae-based biofuels will Total combined fresh and saline water withdrawals in the depend on the cultivation approach (photoautotrophic/ United States as of the year 2005 were estimated at 410. Whether or not this is a problem for sustainable billion gallons per day in 1980. 2009).000 acre-feet carbon feedstock production needing to be part of the per year (Kenny et al. Water use will also depend on brackish coastal water) withdrawals were about 15% of the type of growth systems used for photoautotrophic the total. all of cascading sequence of increasingly saline ponds each with which have increasing energy demand implications (DOE. with water use in upstream organic million gallons per day (Mgal/d). representing etc. one-half of the total U. for future research.. or 460. with the majority (85%) resources. Open algal ponds may from the desalination of saline or brackish water sources have to periodically be drained and re-filled. Kokya et al. and locally available water over 400 billion gallons per day. energy. withdrawals (201. USGS. modeling. Oroud. which also tend to be in the more arid and higher solar resource regions like the Southwest. and coal-bed the past 25 years. Total withdrawals since algal industry scale-up will depend on the geographic the mid-1980s have remained relatively flat at slightly location. 61% of all surface water. solar insolation. 2006b. and 37% of all freshwater withdrawals A significant source of water demand with inland algae (Kenny et al. of non-saline make-up water to dilute the salinity build. Disposal of high not restricted to the more arid western half of the country. Hutchison et use. natural gas. Resources and Siting   .. Almost all saline water. thereby changing fresh water supplies in support of development and the culture conditions. Some salt byproducts may have commercial net fresh water withdrawals that exceed the sustainable value. solar insolation. from pond but is also becoming a local and regional concern at drainage and replacement. 2009). about 95%. and the future expansion of fresh water salinity build-up. 2004. different dominant algae species and growth conditions. or highly adaptive algae that can thrive supplies for non-agricultural use must increasingly come under widely varying conditions. humidity. to cool electricity-generating equipment. Withdrawals water used for cultivation and the local latitude.. withdrawals. This will be of greatest impact and concern in being fresh (Hutson et al. various locations throughout the eastern half of the country.. Also a complicating factor for evaporative water 41% of all freshwater. and field assessment.000 heterotrophic). depending on the chemistry. and 95% loss in open systems will be the degree of salinity of the of all saline-water withdrawals in 2005. water demand over the 30 years prior to that. In 2005. along with associated salt build-up and 2008. salt content effluent or solid byproducts.. 2009).S.S. elevation. Hightower et al. 2002. Irrigation withdrawals represented 31% of all water al. reflects for open pond systems in more arid environments with the fact that fresh water sources in the Untied States are high rates of evaporation. 2008. and operational costs. Implementing water desalination would impose additional The stress on fresh water supplies in the United States is capital. Scoping Out Water Requirements for Algae Production Analysis of U.000 Mgal/d) and weather conditions (cloud cover. periodic addition conservation and by the increased re-use of wastewater. salinity and water chemistry approaching full allocation. closed vs. climate conditions. 2009).uses. management.). 1978. total combined production operations could be for the replacement of water fresh and saline water withdrawals more than doubled continuously lost to evaporation from open cultivation from about 180 billion gallons per day in 1950 to over 400 systems. are important areas supply and management in the future (USGS. or staged as a and from the treatment and reuse of wastewater. 2009). 1995). The relatively flat national water withdrawal trend over produced water” from oil.

held in December 2008. the effects of their extraction on the environment (Alley. quantity. The notional scale-up scenarios and assumptions made are discussed later in this chapter (see Exhibit 9. brackish average algal biomass and oil productivities of nearly and saline groundwater resources in terms of their extent. Dennehy. 2004). reclaimed. 1984) to large area scale-up of algae cultivation (DOE. where brackish or saline groundwater supplies may be The most optimistic production scenario was for the both ample and unused or underutilized. 31 g/m2 per day with 50% dry weight oil content. are also of Along the way. as with other forms of Depth to groundwater is pertinent to the economics of bioenergy (NAS. algal cultivation through harvesting and post-processing particularly deeper aquifers that are largely unregulated into fuels and other products will also be important. warrants aquifers located closer to the surface and nearer to the closer attention and assessment to better understand and cultivation site would provide a more cost-effective source refine water resource requirements. depth information determines the cost of drilling and operating In summary. the use of non-fresh water sources will need open horizontal pan evaporation data (Farnsworth et to be emphasized in the face of the growing competition al. and evaluated under a depth. the estimated capacity. The maps of saline groundwater 9. needed to understand how best to leverage these resources.. Woolhiser and demands on limited sustainable fresh water supplies et al. and may well also be saved. Additional analysis. by state engineers and water authorities. and sustainable withdrawal optimistic productivities are assumed. 1982a and 1982b. and of greater investment. and were based on simply applying fresh water production. Hightower et al.S. Suitable overall input supply needs and consumption. and to predict can be significantly reduced if closed systems are used. limited fresh water supplies.. analysis for algal biofuels.7). other inland could be a consequence of open system operation parts of the country can be targeted for algae production in the more arid and sunny regions of the country. 1985).with open systems was done as part of a hypothetical co-produced water. NAS. southwestern United States. Shuttleworth. and field trials at larger scales of operation are Algae Roadmap Workshop. depending on systems and process specifics. additional water will be used and increasing interest and potential competition for access as consumed. Water requirements of water for algae production than deeper sources located information will not be well characterized until larger scale longer distances from the cultivation site.. Extrapolations based on fresh water From a resource use standpoint. which assumed annual quantitative information remains limited on U. Open bodies of productively using non-fresh wastewater resources brackish and saline water will usually experience less for renewable fuels. resource development. in gallons of water per gallon of algal biofuel produced. with open systems. both in terms of well in a given location (Maxwell et al. discussed be an over-estimate of what can be expected under later in this chapter. There is considerable in the United States are areas of investigation in need untapped potential for utilizing brackish. 2007. and residential development needs in high growth rate Water must be considered a key element of life cycle water-sparse regions like the Southwest (Clark. The location. addition to coastal and possible off-shore areas. Gerbens-Leenes et al. Unfortunately. physical and chemical characteristics. 2006b. define the spatial distribution. If less water quality and chemistry. reducing eutrophication of natural water bodies. Developing and Mapping Water Resources for Algae Production The evaporative loss estimates from the notional scenarios When considering the water resources needed for the provide an indication of the potential magnitude of the future development and expansion of algal biofuel issue. An improved knowledge base is needed to better evaporative water loss intensities will be greater. and sustainable withdrawal rates Evaporative water loss associated with algae cultivation for these non-fresh ground water resources. 2009).. monitored. water utilization for algal biomass and (including energy input requirements for pumping) a downstream production of biofuels. integrating algae pan evaporation data will be worst-case and will likely production with wastewater treatment. has the potential benefits of actual operating conditions in the field. or several hundreds of non-fresh water over a range of salinities means that. depth. and recycling nutrients.. The evaporation estimates suggest that water loss on the order of several tens of gallons of water per kilogram The unique ability of many species of algae to grow in of dry weight biomass produced. 2009). and a source of water for treatment and use to meet commercial recycled. saline. experimental scale-up scenario study in preparation for the National investigation. Along with geological data. Resources and Siting   79 . 1993. and chemical characterization of saline aquifers range of site locations and conditions. Evaluation of water use for the overall value chain from 2003. systems are implemented. as noted earlier. 2008). 2007. Saline groundwater resources. putting less additional demand on evaporative loss than fresh water.

. 2004. longer distance transport and distribution. and analyze this information. 2009). biomass growth rate. content. Flue gas. 2008) characterization of the CO2 emissions stream (e. 2002). separating CO2 in large industrial plants. algae production does possible broader use for carbon capture and storage (CCS) not actually sequester fossil carbon. including Exhibit 9. using coal) represents over 40% of the total. or more than 1965. represent over half of the more than 6 billion metric tons rates and quantities of CO2 produced. The geographical location. including natural Algae production could provide excellent opportunities for gas treatment plants and ammonia production facilities. potential As an example. information is needed to improve our understanding of this resource in support of algae production siting Barriers to Viable CO2 Capture and Utilization analyses (Dennehy. 2005. gas abatement credits would come from the substitution The rate of effective CO2 uptake will also vary with of renewable fuels and other co-products that displace or the algae species. 2008 and 2009). parasitic losses from flue gas the requirements for CO2 supply to enhance algae treatment.000 to 2500 MWe of power would each require many tens of thousands of acres of Carbon Dioxide algae production and large volumes of water to provide a similar effective offset of 20% to 30% of the CO2 emitted. sustainability of supply.resources are based on incomplete data that was largely EIA. Applications fuel-fired power plants. identify. Photoautotrophic algae will only utilize products derived from the algae biomass. local aquifers may be due to CO2 off-gassing during non-sunlight hours and the available through state agencies and private engineering unavoidable parasitic losses of algae production (Brune companies. NATCARB. description of substances toxic to algal growth) and the local availability and distance to suitable land for algae 80 9. 2008 and 2009). at some large growth system and incident light conditions. In addition. typically output a steady 1. Therefore.. However. Resources and Siting   . The sources shown (NATCARB. A limited plant can only be effectively used by the algae during amount of this information is available for major the photosynthetically active sunlight hours. Power generation alone (mainly developed by the USGS prior to the mid-1960s (Feth. a recent analysis suggests that for algae yield. Produced The degree to which stationary CO2 emissions can water from petroleum.. such as from fossil. 2006). recharge rate. 2009). Dennehy. but a significant effort will still be needed to et al. scale of algae production. Any greenhouse CO2 during daylight hours when photosynthesis is active. and coal bed methane be captured and used affordably for algae production wells is another closely related and underutilized water will be limited by the operational logistics and resource that can range in quality from nearly fresh efficiencies. Likely Stationary CO2 Emission Sources Major stationary CO2 emission sources that could An inventory of stationary industrial CO2 sources in potentially be used for algae production are shown in the more promising regions of the country. but rather provides in response to climate change (Rubin. and details of reduce fossil fuel consumption. and the availability of land and water to hyper-saline (Reynolds 2003.. natural gas. for algae cultivation scale-up within reasonable geographic proximity of stationary sources.g. addition. However. Data on small. the utilization of fossil carbon emissions and complement are already in operation today and under consideration for subsurface sequestration. detailed analysis is difficult estimated 20% to 30% of the total power plant emissions and often lacking. collect. aquifers. and the matching of CO2 source availability energy input than the output energy displacement value with algal cultivation facilities. including algae production. USGS. 2008b). and distribution could require more production.200 acres of algae cultivation resource availability and suitability for economic uses area (Brune et al. spatial extent. Campbell carbon capture and reuse in the form of fuels and other et al. if these aquifers are spread over the greenhouse gas emissions offset will be limited to an large geographic areas. Larger coal-fired base-load generators that locate. it will be necessary to provide a CO2 source that is suitably free of materials potentially toxic to algae. The CO2 generated by the power (Shannon. The Carbon Capture Opportunity in Algae Production Efficient algae production requires enriched sources of CO2 The distance for pumping flue gas to algae cultivation since the rate of supply from the atmosphere is limited by systems will become a limiting factor that requires diffusion rates through the surface resistance of the water capture and concentration of CO2 from the flue gas for in the cultivation system. transport. depth. and of CO2 emitted annually in the United States (EPA.5. 2009. 2008). More detailed and up-to-date 2 billion metric tons per year (EIA. In represented by the algae biofuels and other co-products. As a result. would be a good source of CO2. is not a simple issue. and quality production to fully utilize the CO2 in the flue gas emitted (chemical components and characteristics) are important from a 50-MWe semi-base load natural-gas-fired power information for assessing non-fresh groundwater aquifer plant would require about 2. 2004).

6.5 Major stationary CO2 sources in the United States (NATCARB..g. Resources and Siting   81 . Exhibit (USDA. any given region unless other factors drive the investment in expanding the nation’s CO2 pipeline infrastructure.796 9. 2006 and 2009).production. Soils. This of land for algae production (i. and particularly their porosity and permeability Land characteristics.5) within practical transport distances of suitable algae and relatively barren desert land. Publicly and privately owned lands are algae culture surface area to produce 10 million gallons of subject to variable use.3 140 Cement Plants 86. and purchase requirements. 9.e. 2008a) CO2 EMISSIONS CATEGORY NUMBER OF SOURCES (Million Metric Ton/Year) Ag Processing 6.2 475 Gas Processing Refineries/Chemical 196. affect the construction costs and design Factors for Evaluating Land for Algal Production of open systems by virtue of the need for pond lining or Land availability will be important for algae production sealing. is needed for making refined assessments for Land availability is influenced by various physical. For example. Topography would be a limiting factor for these because either open or closed systems will require systems because the installation of large shallow ponds relatively large areas for implementation. For a realistic appraisal production sites in a given geographical region. target annual average biomass production of 30 to 60 g/m2 per day with 30% to 50% Land ownership information provides valuable insights neutral lipid content on a dry weight basis).5 3.6 53 Petroleum and Natural 90. such systems on which policies and stakeholders could affect project would require in the range of roughly 800 to 2600 acres of development. grassland.. algae production siting and CO2 sourcing.g. Even slope could well be eliminated from consideration due to at levels of photoautotrophic microalgae biomass and oil the high cost that would be required for site productivity that would stretch the limits of an aggressive preparation and leveling. including pasture. Hundreds of millions of acres of relatively low- the limited quantity of CO2 that would likely be available productivity. social. several characteristics need to be considered. as will be discussed further in chapter 10.0 13 Industrial 141.. Physical characteristics. lower-value land exists in the United States from stationary industrial point sources (e. which means that environmental assessments and/ Exhibit 9. such as topography and soil.276. R&D program (e.9 665 Other 3.1 4.9 173 Total 3. One outcome economic. could limit the land available for open pond algae farming.002 Ethanol Plants 41. as is expected requires relatively flat terrain. oil feedstock. as illustrated in of a hypothetical algae production scale-up scenario is Exhibit 9.3 163 Fertilizer 7. and political factors. lease.3 112 Electricity Generation 2. land that would be both can be expected to constrain the extent to which algal suitable and potentially available for siting algae production biofuels production can be affordably scaled up within facilities). Areas with more than 5% with any photosynthesis-based biomass feedstock. legal. much of the land in the West is government owned.702.

Loss mechanisms that often not considered or is relatively small compared to other capital cost. given that large-scale algae biomass production The result will be theoretical and practical upper limits on intended for bioenergy feedstock does not yet exist. 1985 ) 82 9. Optimizing light utilization in algae production projections for biofuel feedstock of 30 . system structures.. the dissipative heat loading Land Availability Constraints can be a benefit in moderating culture temperatures and Land use and land value affect land affordability. Resources and Siting   . can represent and impose political and regulatory Despite the practical upper limits that will naturally exist constraints on land availability (Maxwell et al. such systems have not been optimized for energy losses that occur when incident photons that cannot higher-volume. lower-value production with algal strains otherwise be effectively captured and used by the algae developed and improved over time to be more suitable and in photosynthesis are instead converted to thermal (heat) productive for biofuel feedstock rather than those used energy in the culture media and surrounding cultivation for today’s high-value algae biomass product markets. systems includes the challenge of managing dissipative However. Indian reservations also effectively used to drive photosynthesis ultimately places comprise a significant portion of this land. 2009). Land ownership practical upper limits on the biomass productivity. algae productivity crops. reviewing the more recent economic analyses for algae or can lead to overheating and loss of productivity biomass and projected oil production. By improving productivity under colder ambient conditions. It must be stressed that “potential” will be constrained by the available energy density algal biomass and bio-oil production projections at in sunlight and the relatively low efficiencies of commercial scale currently remain hypothetical rather photosynthetic processes coupled with other systems losses. the amount of biomass growth that can be achieved per unit Relatively large-scale commercial algae production of illuminated surface (Weyer et al. but currently reflect lower biomass fact that algal cells nearest the illuminated surface absorb productivities in the range of 10 . the cost of land is during hotter ambient conditions. than real. 1985).. as with any form of biomass.6 Factors and process steps for evaluating and constraining the available and appropriately suitable land for algae production (Adapted from Maxwell et al. This the light and shade their neighbors farther from the light is significantly lower than the more optimistic target source. (chapter 10). Contributing to with open ponds for high-value products can serve as a productivity limits per unit of illuminated surface area is the baseline reference..60 g/m2 per day. for algae productivity.20 g/m2 per day. Depending on the algae strain and culture system approach used.or environmental impact statements could be required restrict the fraction of incident photon flux that can be as part of the approval process. Land that is highly desirable for development and other set-asides for publically beneficial reasons may Typography Land Ownership Current Land Value Systems Engineering All Physically Politically Land Suitable Affordable Available Available Parcels Land Land Land Land Enviromental Restrictions Current Land Use Legislation Water and and Regualtions Climate Resources Exhibit 9. the potential remains for high algae biomass production relative to more conventional Further.

excess nutrients (e. phosphorus. Beyond economics. treatment has the following advantages: and other historical sites. On the other hand. • Imported wastewater provides nutrients and water to make-up inevitable losses. wildlife areas. and potassium) be a key issue for achieving affordable and Algae can be useful in the treatment of waters polluted with sustainable scale-up of algae biofuels production. Resources and Siting   83 . organic matter.g. biomass production facilities 1985). wastewater treatment and recycling • Potential to be integrated with power plant must be incorporated with algae biofuel production. Examples of this type of constraint include technologies. The imported wastewater Wastewater Treatment and would be treated as part of the algae production.. Supply and cost of nutrients (nitrogen. Recycling Applications • Algae-based wastewater treatment provides a needed Municipal wastewater treatment facilities and agricultural service. synthetic organic compounds. archaeological sites. nitrogen. or other CO2-emitting industry operations The main connections of algae production and • Potential to treat agricultural drainage wastewater treatment are the following: and eutrophic water bodies • Treatment technology is needed to recycle nutrients and water from algae biofuel processing residuals for use in algae production. Two main types of algae production facility are wastewater (municipal and agricultural) would be captured envisioned: dedicated facilities. represent potential co-location sites for algae training and experience in large-scale algae operations where nutrient-rich wastewater could be cultivation that would translate to future dedicated used for algae production. for additional rounds of algae production. help provide nutrient removal service in the wastewater For large-scale algae biofuel production. as noted earlier. The same applies to land highly suited for higher-value agricultural POTENTIAL BENEFITS OF ALGAE PRODUCTION use. A subset of wastewater treatment Inevitable nutrients losses during algae production facilities consist of evaporation facilities. 9. particularly in the eastern half of the deployed in the near-term and provides workforce country. phosphorus.not be seen as suitable for algae production. and potassium). and the algae production can algae feedstock production facilities. some land • Early opportunity to develop large-scale cover characteristics could present excellent opportunities algae production infrastructure for algae farming. offsets algae production costs • Lower capital and O&M costs than 9. in suitable climates. The availability and sustainability of water supplies • Wastewater treatment revenue that in the West will also be a key consideration. nutrients from treatment. Land cover categories such as barren and scrubland cover a large portion of the West and may • Development of skilled algae production workforce provide an area free from other food-based agriculture • Potential for nutrient recycling at algae where algae growth systems could be sited (Maxwell et al. biofuels industry are discussed in this subsection. metals.. nutrient recycling.. and wastewater treatment facilities. algae-based parks. dairy and feedlot operations located throughout the • Algae-based wastewater treatment can be United States. Sensitive environmental or cultural land constraints Although algae-based wastewater treatment requires will also reduce the overall land availability (Maxwell many times more land area than mechanical treatment et al. with the main purpose of by algae and then recycled from the oil extraction residuals biomass production. The roles of nutrients. Dedicated biomass production the United States are insufficient to support large-scale facilities will also require wastewater treatment and algae production on the basis of a single use of nutrients. which are and processing could be made up with wastewater used to dispose of wastewater or brines. 1985). this also avoids the perception WITH WASTEWATER TREATMENT and potential conflict of food and feed production versus fuel. monuments. Nutrient which produce algal biomass as a consequence of the recycling would be needed since wastewater flows in wastewater treatment.4 Integration with Water conventional wastewater treatment Treatment Facilities • Lower energy intensity than conventional wastewater treatment (a greenhouse gas benefit) Inevitably. which can also help supplement and off-set these facility types in the development of an algae the cost of commercial fertilizers for algae production.

natural disinfection and remove trace contaminants. and naturally occurring organic catalysts (Sinton et al. 1988. followed by algae separation in dissolved treatment are aligned.. and lakes). Borde et al. The (e. food processing). harming higher organisms. organic nutrients.g. to oxidize waste organic matter (Exhibit 9. 1998. coupled with the tremendous need for expanded and improved wastewater treatment in the United Treatment of Organic Wastewaters for Algae Production States (EPA. of chemical addition ($0. cost savings.. This nutrients (e. These highly productive systems use shallow reactors. but high productivity is not crucial to the (Borde et al. the method is chemical addition to achieve coagulation and objectives of biofuel feedstock production and wastewater flocculation. need high algae from animal facilities) is an area in need of study. High rates of algae production lead to high rates crucial step in wastewater treatment systems.. confined animal facilities). including wastewater treatment ponds. Finally.10 .$0. eventually unlikely when wastewater contaminants are present. 2008) and throughout the world. Algae are similar to in conjunction with wastewater treatment.17 per m3 treated) or manipulation of culture conditions to promote lipid (Maglion. needed if algae are to make a substantial contribution to Less well-known are the ability of algal systems to provide national biofuel production. high-productivity algae and water treatment are those contaminated with organic ponds have a total cost that is about 70% less than activated matter and nutrients (e.and potentially endocrine disrupting compounds (Oswald. 2009).7). endocrine disrupting matter and pathogens only).g. the major types to build and to operate than conventional mechanical of wastewaters available for combined algae production treatment facilities. Resources and Siting   . Mechanical treatment technologies typically hold either high rate ponds (~30 cm) or algal turf scrubbers the wastewater for less than 12 hours. organic agricultural mechanisms of wastewater treatment by algae. 2003.. 2005). The standard of nutrient removal and wastewater treatment. 2009.. The treatment goals of these facilities (removal of organic removal of trace contaminants (e. and eutrophic waters dissolved oxygen algae release is used by treatment bacteria with low organic content but high nutrient content (e. to make up for nutrient losses (Brune et al. productivities (as does biofuels feedstock production).. Heavy metals may be removed Algae Production Techniques for by adsorption to algal cells. recycling of concentrations is useful in wastewater treatment. which is the leading water treatment technology and wastewaters mainly contaminated with inorganic used in the United States (Downing et al. 2002. These reactions are also the best-known industrial (e. The cost biomass production. The bioaccumulation of trace contaminants in high-value products (>$100/kg biomass). lakes. the is illustrated schematically in Exhibit 9. 2002). As noted above. harvesting is a 2002). whereas pond (~1 cm). Existing interaction of algae and bacteria in wastewater cultures algae-based treatment facilities use relatively deep leads to degradation of a wide variety of synthetic ponds (1-6 m). As with other algae production systems. agricultural drainage. Muñoz et al. Maintenance of lipid-rich strains. The great depths contribute to low algae organic compounds such as phenol and acetonitrile productivity. 2008) is high for biofuel production... Ponds for more advanced compounds such as human hormones and antibiotics treatment. Woertz et al.. might be prevented to a 84 9.g. Thus. municipal and industrial sources) sludge. For example. dissolved oxygen. and rivers). Even with internal recycling. which will be a benefit as Wastewater Treatment Plants long as the resulting metals concentrations in the algae biomass are not excessive or inhibitive for later use in Integration of algae production with wastewater treatment the processing of fuel and other co-products.g. which is algae that would occur in the receiving waters.7. Closed photobioreactors are not emphasized technologies hold the wastewater for at least several days in this wastewater treatment discussion since they are and in an environment similar to many natural receiving likely to be economical only when also producing waters. The ability agricultural drainage. at least in terms of maximizing air flotation units or sedimentation clarifiers. autoflocculation) are far less costly. Non- production. rivers. Kohn et al. provides a Organic-rich wastewaters usually also contain nutrients.. practical opportunity to install algae production facilities requiring two types of treatment. The major plants in that they both produce oxygen and assimilate classes of wastewaters to be treated are municipal. if the nutrients and carbon at algae production facilities will be nutrient-rich algae are then also removed from the water. Despite a seeming of algae to assimilate dissolved nutrients down to trace abundance of wastewater and waste nutrients.... have yet to be demonstrated consistently chemical flocculation processes (bioflocculation and for ponds..g. including nutrient removal. 2007). but research is needed to improve the reliability of these processes (as Algae-based treatment facilities are typically less expensive discussed in chapter 4). Aksu.g. importation of wastes and/or wastewater will still be Disinfection is promoted via the production of oxygen needed in dedicated algae biomass production facilities radicals in the presence of sunlight.

High Rate Clarifier Clarifier Pond Raw Sewage Treated Effluent Sludge CO2 Digestor Generator kWh Food Algae Biomass Production Inputs Option Liquid Biofuel Products Reclaimed Water Fuels Fertilizer To Fertilizer b) Simplified conceptual system block diagram 9.Exhibit 9.7 Integration of algae production with wastewater treatment for nutrient removal and biomass production: Sun CO2 Reclaimed Algae Water CO2 N P Waste CO2 O2 N Biomass Water P Organics Bacteria a) Basic principles of operation. Resources and Siting   85 .

Salton Sea.. and potential algal turf scrubbers without CO2-addition and high opportunities for the federal government. For all biofuel wastes for algae production feedstocks. etc..g. market from decomposition of organic matter would not be drivers. 2002).g. but further investigation is techniques. calls. as follows: • Large-scale (3-5 acre) demonstration of CO2- enhanced high rate ponds for nutrient removal • Determine CO2 biofixation efficiency 86 9. co-products. etc. of several issues. Resources and Siting   . research efforts and policy evaluations would likely need to focus on Main Research Needs for Algae both carbon capture and the production of biofuels and Production with Wastewater co-products to overcome barriers (technical. low-organic content wastewaters will expand Cultivation Facilities with the opportunities for algae production systems. Furthermore. CO2-Emitting Industries Agricultural drainage and eutrophic water bodies (e. Lundquist et al. technical and market challenges. such as bioflocculation. waters is likely to occur in more rural settings than and additional input sought from managers at major treatment of municipal wastewaters. Mulbry et al. routes of such contamination should be • Determine the cost savings and greenhouse gas studied and preventative measures developed. These follow-on efforts were coordinated with the Electric Power Research Institute (EPRI). Findings from rate ponds with CO2 addition has been demonstrated in these interviews and conference calls were integrated California’s Central Valley and elsewhere (Craggs et with the Workshop inputs in developing this section. al.. The high rate ponds could create theme was that electric utilities primarily view algae an algal product while performing the service of water cultivation as a means of CO2 capture as opposed to a evaporation... constraints significant. needed to confirm this. Treatment of agricultural drainage with on large-scale development. Calif. mine drainage. potentially increasing • Demonstrate recycling of biofuel processing the cost of waste disposal or recycling. any heavy metals autoflocculation. Thus. contaminating the algal biomass likely would remain in the waste from biofuel processing.) are examples of such waters This section includes findings from discussions held at the (Benemann et al. desired absorption is essential since inorganic carbon generation outcomes.. regulatory Successful use of high rate ponds specifically for and economic) for algae facilities that are co-located nutrient removal/recycling requires resolution with electric utilities and other industrial CO2 sources. integration opportunities and challenges. 2004). Treatment of nutrient-rich National Algal Roadmap Workshop break-out sessions. As with any evaporation pond technology companies and fuel refiners/distributers with system. hazards to wildlife from toxic compounds (e. their ability to treat high. very different business models and goals for algae selenium. Evaporation ponds are currently used to method for producing biofuels and co-products. micro-screening. production in order for this type of co-location to be widely commercialized. potentially leading electric utilities through later meetings and conference to greater land availability and savings in land costs. It is important to point out that amongst the numerous barriers to co-location of algal cultivation facilities with High rate ponds might be used as components of industrial CO2 sources identified at the Workshop and evaporation systems needed to dispose of blow-down subsequent discussions with electric utilities. an overriding or other wastewater. However. 9. The processing of the algal biomass for fuel • Develop algae grazer control strategies and other co-products would presumably destroy and • Develop reliable low-cost algae harvesting neutralize the contaminants. chromium) must be carefully evaluated. dispose of agricultural drainage. The topics wastewaters. 2008. Treatment of Inorganic Wastewaters for Algae Production In addition to the ability of algae systems to treat organic-rich wastewaters. 1996. CO2 addition or slow atmospheric of discussion included the value proposition. (GHG) emissions avoidance benefits compared to conventional wastewater treatment technologies. and included For algae-based treatment of low-organic content several large municipal electric utilities.great extend by pond treatment followed by algae • Determine growth model parameters harvesting.5 Co-location of Algal nutrient. oil field produced electric utilities may need to partner with algae cultivation/ water.

but economic viability under a broader range of geographic and climate must be determined case by case. fossil fuel require establishing a U. This higher concentration would affect Coal-fired power plants may be a convenient source of the sizing and operations of algae production facilities— CO2 for algae production. introduced earlier in Exhibit on the phasing of the intermittency with respect to the 9. Co-location of algal cultivation with industrial available. shows that fossil-fired power plants represent daylight hours when photosynthesis is active. while providing While the information in this section focuses on fossil. However.S.15%) than natural gas plants (5 . fermentation-based industries. The Techno-Economic Assessment section of this report. A number of large coal-burning power plants with baseload coal-fired plants. and distance of transport to algae management of algae cultivation systems – this cultivation sites. it is also relevant to other CO2-intensive • Potential carbon credit for utilities. Costs are expected to be lower than for will allow developing algal cultivation facilities pure commercially supplied CO2. The growth. fired power plants.g. The emissions from fuel in lieu of permanent sequestration. coal-fired plant flue gas typically expected to result in a net reduction of overall GHG has about a factor of two greater CO2 concentration emissions.The Opportunity in Co-Locating with CO2-Emitting Sources ADVANTAGES OF CO-LOCATION OF Since photoautotropic algae growth requires CO2. carbon sequestration. some absorption and re-use as transportation geothermal power production. reason is that coal-fired power plants have higher CO2 emissions per unit energy produced than natural gas-fired An important policy question to consider is the value power plants. which typically ranges from about 5% to about 15%. The regulatory implications transport. the CO2 would be emitted to the atmosphere if not captured and sequestered by other means (Rubin. and ALGAE PRODUCTION WITH STATIONARY productivity can be enhanced by supplementing the INDUSTRIAL CO2 SOURCES limited CO2 available from the atmosphere. gas-fired power plants and fuel companies are likely to widely adopt algal that operate as peaking plants rather than base-load cultivation co-located with industrial CO2 sources. Resources and Siting   87 . the algae through photosynthesis. but. using algae to capture the maximum of CO2 absorption by algae in any carbon-credit or amount of CO2 emissions from coal-fired plants would cap and trade framework. During those times. generators will have intermittent operation that would A variety of stationary industrial sources of CO2 introduce intermittency in the supply of CO2 for algae are distributed throughout the United States.).5. beneficial re-use of cooling water and wastewater.. many of these facilities have higher CO2 concentrations compared to power plant flue gas. plants – overcoming a primary resource challenge for algae cultivation at scale. Offset of fossil fuel conditions on or near a year-round basis. cement manufacturing. This will industries (e. 9. Gas-fired the majority of CO2 emissions from stationary baseload generators would not be intermittent. depending on the type of plant and fuel used. conditioning. construction of algae systems at natural models described in more detail in the Systems and gas-fired power plants may be a better investment. While algae biofuels can be produced. concentrated • Abundant quantities of concentrated CO2 available sources flue gas from fossil-fuel burning power plants from stationary industrial sources can supplement and other CO2-emitting industrial sources can be low concentration CO2 from the atmosphere. etc. the process of capturing flue-gas CO2 to make (10 . Resulting costs for • Excess heat or power may be available to provide CO2 will be site-specific and dependent on methods of heating or cooling for improved thermal capture. would also emit CO2 distributed across the southern tier states provide during periods of darkness when it cannot be utilized by ample sources for algal growth on a large scale. found often in proximity of power CO2 sources is a promising area for further research. The impact on algae production would depend quantitative breakdown. in that the carbon will be require proportionally larger algae production systems re-released to the atmosphere when algal-derived per unit energy produced and higher costs per unit energy fuels are combusted.6%). 2005). beneficially used in algae production. Thus. as sources. and delivery of CO2 from the power plant to of this will need to be addressed before utilities the algae cultivation site. but from an emissions control an aspect that could be incorporated into engineering perspective. which can transportation fuels may not rigorously be considered bring some advantage in terms of efficiency of capture. consumption by algae biofuel and other co-products must be done with approaches that provide a net GHG emissions • Excess wastewater or cooling water may be reduction. Also. policy on carbon extraction/refining.

mechanisms to encourage partnering CO2 sources and nutrient sources such as wastewater between utilities and algae/fuel companies will be treatment facilities and agricultural waste streams.). Thus.10 acres). and new business models will be needed to • Unclear regulatory framework for carbon-capture commercialize this approach. These costs and losses must be parasitic losses of CO2 transport. ethanol plants or other and optimize algae production will not be “free”. universities. The use of flue gas to cultivate algae will involve non-point source emissions at ground level. minimized and compared to other approaches for the ▪▪ Evaluation of temperature control (power capture and sequestration or reuse of carbon.. or in some cases algal to capture CO2 as opposed to producing biofuels and cultivation could be co-located with both stationary co-products. Specific test-bed R&D absorbed during periods when sunlight is available topics relevant to power and wastewater utilities and photosynthesis is active in the algae. and facilities that will aid the following: are based upon point-source emissions from high ▪▪ Rapid and consistent engineering design elevations. Therefore nutrients must be production arena. Current plant cooling and algae pond heating) estimates are that approximately 20% . The same generally holds true for other cultivation companies. and agricultural • Too much CO2 near plants for realistic absorption: drainage/water body restoration sites that also represent Large power plants release too much CO2 to a range geographical locations. etc. credits: Until there are regulations in place that quantify carbon credits from algal growth facilities. advantages to capture and deliver industrial CO2 to ponds and of decentralized production considering grow/harvest algae. will be required for commercialization of may not be available adjacent to or near major algal cultivation facilities co-located with industrial power plants CO2 sources and/or wastewater treatment facilities. required. ethanol plants. to conduct research at the pilot scale (3 . Although often referred to as a “free” resource. stationary industrial sources as a supplement to enhance wastewater treatment facilities. the capture and delivery of concentrated CO2 from The test-beds would ideally be located at power plants. distributors. CO2 emitting industry facilities. Several areas for research. K) supply. Also. ▪▪ Techno-economic analyses • Capital costs and operational costs: There exists a ▪▪ Life Cycle Analysis and GHG abatement analysis need to evaluate capital costs and parasitic operational ▪▪ National inventory of potential production sites losses (and costs) for infrastructure and power required ▪▪ Evaluation of economies of scale vs. solar resource. P. • Emissions from ponds are at ground level: Regulatory The following directions have been identified: requirements from power plants and other stationary • Develop computer models of algae production sources are governed by the Clean Air Act. • Establish national algae biomass production test-beds 2009). the uncertainty may pose a barrier for wide commercial Directions for Research and Development adoption of the technology. algal facility.30% of a power plant’s greenhouse gas emissions can be offset ▪▪ Development of efficient test-bed facilities by algae biofuel and protein production (Brune et al. Resources and Siting   . as well as policy-development • Land availability: Suitable and affordable vacant land efforts. Their fundamental objective will be brought in from other sources. algal technology companies. sources of concentrated CO2 can potentially provide • Resistance from electric utilities: Electric utilities are ample carbon for photosynthesis-driven algal growth. This effort could involve a likely to be possible near the power plant consortium of R&D organizations. etc. stationary industrial sources of CO2 (cement refiners. and other participants coordinated plants.Barriers to Co-Location of Algae • Maintaining cultivation facilities during utility outages Production with Stationary Industrial and through seasonal changes in algal growth rates: Detailed models will be needed to develop and evaluate CO2 Sources approaches for managing the variable nature of both • Need for nutrient sources: While stationary industrial CO2 emissions and algal growth rates/CO2 uptake. CO2 is only at the national level. not in the fuels business and regulated public utility in most cases there will not be a complementary commissions will be constrained in entering the fuel nutrient (N. include: ▪▪ Technology evaluation at larger scales ▪▪ Determination of algae production facility model parameters 88 9. and be absorbed by algal ponds at a realistic scale climate conditions.

proteins: carbohydrates and C:N:P) ▪▪ Power plant cooling with treated wastewater in ▪▪ Methods of nutrient and water recycling within conjunction with algae production production facilities. etc. and absorption/biofixation efficiency emissions from ponds including plume modeling and algal biomass productivity given seasonal and and regulatory analysis diel variations in photosynthesis and various ▪▪ Effects of various flue gases on algae production water chemistries and co-product quality ▪▪ Control of algal biomass quality (ratios of lipids: ▪▪ Scrubbing of flue gas for NOx. flocculation harvesting. SOx. crop fertilization. costs. Develop university training programs. salinity and blowdown • Evaluate policies that would encourage partnering management. between public utilities/other industrial CO2 sources ▪▪ Algal biomass handling. Resources and Siting   89 . and processing and algal cultivation/technology companies prior to fuel extraction. ▪▪ Development of algal strains and their cultivation techniques 9. and refiners/distributors.▪▪ Flue gas or other industrial source CO2 supply ▪▪ Investigate the safety of ground-level flue gas logistics. and other sites. storage. pathogen safety • Develop and train the future algae production/algae ▪▪ Beneficial management of residuals for soil carbon biomass processing workforce at the national test-bed development. etc.

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Reynolds.. Ahmadzadeh B. Impact of chemistry on sustainable water with its nexus to the economy. National Academy of Sciences. 9. Treatment of dairy manure effluent using freshwater algae: Algal productivity and recovery of manure nutrients using pilot-scale algal turf scrubbers. 2005-2012. and Meyer. E. N.K. (2008)...Y. Proposing a formula for evaporation measurement from salt water resources.A. Hydrological Processes.K. Microalgae and wastewater treatment.E.). Hightower. I. J. Graham.L. E. National Academy of Sciences (NAS). (2004). (SAND 2007-1349C). Association with natural organic matter enhances the sunlight-mediated inactivation of MS2 coliphage by singlet oxygen. J. F. Photosynthetically oxygenated acetonitrile biodegradation by an algal- bacterial microcosm: A pilot-scale study. 99. (1965). and Mattiasson..F. Oklahoma Geological Survey.. T.C.R.. Muñoz. 12). Green. Davidson.. (2004). Hutson.L. Cambridge University Press. NM. C. A. Pate. (2008). Modelling the water and salt balance in a shallow lake. B. Carbon Dioxide Capture and Storage. W. K. Washington. and Terry. Folger. McNeill. Estimated Use of Water in the United States in 2000. Pizarro. S.. 22.. Water implications of biofuels production in the United States. Feinberg. energy.J.000 parts per million dissolved solids. (2008). (SERI/SP-231-2550). Rubin. (Atlas HA-199). The energy challenge: Commentary.. (1982). C... (1986). Lumia. Oroud. and research requirements. M.natcarb. (SERI/TR- 215-2484). Washington. Turhollow. and de Coninck. and Maupin. The National Academies Press. 41(13). Kondrad. Feth. Department of Agriculture. Kokya. (2003). R. Hutchison. D. Geological Survey Hydrologic Investigations. (2007). Erbach (2005). de Connick.. (2006). CO2 Source Map of US . B. and Nelson. and Oswald. (2007). K. 51(12).A. Theoretical and Applied Climatology. (SERI/TP. Golden. Biomass as feedstock for a bioenergy and bioproducts industry: The technical feasibility of a billion-ton annual supply. L.. (1990).natcarb. Nature. S. CO. and Einfeld. R..J. Hightower. E. E. and Maupin M. R. Feinberg. Linsey. M. M.. S.. (DOE/GO-102005-2135. and Kebede-Westhead. Los Alamos. University of Illinois.. Solar Energy Research Institute. (1995). R.. J.CO2 sources were compiled by NATCARB http://www. Kenny. potential. CO2 sources for microalgae-based liquid fuel production. S. (2005). Preliminary map of the conterminous United States showing depth to and quality of shallowest ground water containing more than 1. Denver. M. (USGS Circular 1268). N. U. 4. Resources and Siting   91 . Available online at: http://pubs. CO. Lynn L. Stokes.S.. California Department of Water Resources.. NATCARB (2008a). B.E. T.html Neehan.S. Cambridge University Press. Fuels from microalgae: Technology status.F. K. Rolvering.. Effects of salinity upon evaporation from pans and shallow lakes near the dead sea. Technical summary...S. Bryce J.J. H... U. Barber.W.H. McIntosh. E. and Hogg. Quinn. 452. Wright. 4626-4632. California. Center of Advanced Materials for the Purification of Water with Systems. D..K. Borglin. and Donald C. (1988). In Metz. R.. Microalgal Biotechnology (chap. Solar Energy Research Institute. 211-235. Hutson. Water Science Technology.. CO.C. Sandia National Laboratories. and Kokya. (1978). Golden.G. K. Solar Energy Research Institute. Golden.usgs... Kohn. S.html NATCARB (2008b). Kenny. R. D.. Department of Energy. Cameron.. 8137-8142. L.. Linsey. C.Farnsworth. Development of Drainage Treatment for the San Joaquin River Water Quality Improvement Project. (Eds).P. T. I. Environmental Science & Technology.S. 261-265. Maxwell. Hsieh. Robin L. D. Shannon.G. N. Robert D. Thompson. W. M.L.. K. Produced water and associated issues. Mulbry. and Peck. H. and Karpuk. United States Geological Survey. Lundquist.. M and Pierce.S.L.T. D. M. Overview of energy-water interrdependencies and the emerging energy demands on water resources. UK. Loos. (USGS Circular 1334). 52. Resource evaluation and site selection for microalgae production systems. OK. (2009).A.. and Midgley.L. Bioresource Technology. Norman. Saline aquifer data 231-240. In Borowitzka and Borowitzka (Eds. (OPEN-FILE REPORT 6-2003). A. O. and the environment. J. DC.B. S. Lovelace... (1985). (2005). Fresno. Guieysse. Evaporation atlas for the contiguous 48 United States. W. B..S. W.M.S. D.232-3820).C. Grandbois.. ORNL/TM-2005/66). 285-286. Perlack. Ecological Modelling. (2007). Estimated use of water in the United States in CO. Barber.. Hill. Oswald.. Meyer. C. (NOAA Technical Report NWS 33) Washington. Anthony F.

H.J. I. (ANL/ ESD/09-1) U.S. 135(11). Bush. 92 9. R. and Davies-Colley. Feffer.. and management: Major uses of land. 1–10.S. Argonne National Laboratory.R.J.S. Y.A. Estimated use of water in the United States in 1995. M.. (1984).usgs.. Weyer. and Perlman. J. Solley.. Applied Environmental Microbiology. 1115-1122. C. (2002). Land use.S. (Ed). 110(2).ers. Benemann. Shuttleworth. Produced Water Quality Map of US http://energy. (2009). 246-250.S. Mintz... 68(3). Hall. T. M. (2009.. (1993).S. (2009).usda. Evaporation. R..). T. K. McGraw-Hill. Climate change and water resources management: A federal perspective. (Economic Information Bulletin Number 14). (U. W. (2009). Department of Agriculture (USDA).W. value.. USDA Economic Research Service..htm U. Darzins. Department of Energy’s aquatic species program--biodiesel from algae. Lynch. Lundquist. Resources and Siting   . 1993. (NREL/TP-580-24190).A. W. Woolhiser. (USGS Circular 1331). Golden..H. (2002). (1998). R. (2006).J. D. Handbook of Hydrology (chap. Dunahay. CO.. B. 4).A. Geological Survey Circular 1200).E. Available online at: http://water. National Renewable Energy Laboratory. Theoretical Maximum Algal Oil Production. BioEnergy Research.. A. Wu. Lipid productivity of algae grown during treatment of dairy and municipal wastewaters.G. Geological Survey. J.htm U.R. M. Sunlight inactivation of fecal indicator bacteria and bacteriophages from waste stabilization pond effluent in fresh and saline waters. 2002. Wang.usgs.1122-1131. and Wallace. and Arora. P. D. Geological Survey. A. Pierce.. Major uses of land in the United States.. U. USDA Economic Research Service On-Line Briefing Room Available online at: http://www. Sinton. Journal of Irrigation and Drainage Engineering. (2009). Consumptive water use in the production of ethanol and petroleum gasoline. Woertz. L. and Roessler. Department of Agriculture (USDA). US Geological Survey. D. Mapping average daily pan evaporation. M. (1998). In Maidment.. A look back at the U. & Willson.M. Journal of Environmental Engineering.. D. and Nelson.

systems. can commercialization of the still relatively immature provide informed guidance for targeting investments in field of algal biofuels also requires the confidence and select technologies. e. comparative cost per Techno-Economic Analysis gallon of biofuel for different pathways Given the numerous potential approaches and pathways available for algae biofuels and co-products (as illustrated in Exhibit 10.2.g.1 High level illustration of various approaches and pathways to developing algae-derived biofuelsand co-products ALGAE Species Unicellular Multicellular Selection & Cyanobacteria Microalgae Macroalgae Biology Open Terrestrial Closed Terrestrial Open Offshore Cultivation Systems Systems Systems System: Hybrid . techno-economic modeling and analysis can across the overall algae-to-biofuels supply chain. Systems modeling and analyses technical innovations in systems and processes. comparative yield and energy balance for different pathways • Assess economic impact of R&D strategies and 10. processes. significant investment is expected to be required to overcome the various technical and economic Exhibit 10. coupled applied across this space and done at different levels of with economic feasibility in the practical implementation precision and granularity. environmental. tradeoffs among different technologies. and market conditions and constraints. Toward this end. It can also help track performance and they can make necessary investments over time to reduce progress (technical.Harvesting Systems . as discussed co-products industry requires the optimum combination of throughout the Roadmap. Enabling successful advancement and or national resource and supply chain assessment. Systems and Techno-Economic Analysis Successful development of an algae-based biofuels and challenges along each of these pathways.Separation Hydrocarbons Consolidated Intermediate Lipids Carbohydrates Proteins & Alcohols* Biomass Constituents * Biologically produced in-situ by the organism Biochemical Thermochemical Direct Anaerobic Conversion Conversion Conversion Synthesis Digestion Processes Renewable End Use Fuels Biodiesel Alcohols Biogas Co-Products & Products Hydrocarbons 10. to an overall regional and marketing. and processes. e. economic. Such serve a range of purposes and goals that include: analyses can provide improved understanding and insight to help guide successful industry development within the • Assess technical performance and cost/benefit real-world context of technical. ranging from individual unit and integrated scale-up for commercial production operations to an integrated enterprise..1 Objectives of investments. Systems and Techno-Economic Analysis  93 .Dewatering . infrastructural.10. and environmental) critical technical risks and overcome challenges to developing to meeting performance objectives and reaching goals for an algal biofuels industry.g..Fractionation Phototrophic Mixotrophic Phototrophic Heterotrophic Phototrophic . objective successful commercialization. as illustrated in Exhibit 10. and deployment engagement of key public and private stakeholders so approaches. systems. political.1). and quantitative modeling and analyses of systems and processes are needed that span different enterprise levels In short.

• Assess environmental impact of R&D strategies 10.2 TEA Scope and Methods
and investments, e.g., comparative net greenhouse
gas (GHG) emissions per gallon of biofuel for Exhibit 10.3 illustrates at a high level the essential
different pathways elements of techno-economic modeling and analysis.
• Assess consequences and constraints of alternative These intersecting elements present a broad systems
pathways for algal feedstock, biofuels, and co- perspective that integrates the interdependent science
products industry build-up, e.g., land, water, CO2, and engineering aspects specific to developing algae
other nutrients, energy, and infrastructure. biofuels, keeping environmental, economic, and
policy aspects in view to provide critical insight
• Inform R&D and business development investment
and information needed for decision-support.
• Inform policy decisions and explore “what if” Techno-economic assessment for algae biofuels is
scenarios highly interdisciplinary and will range from GIS-based
• Provide insight and actionable information needed resource and siting assessment, to engineering modeling
to successfully guide technical advancement, of systems and processes at the unit operations level, to
integrated system scale-up, and commercialization interdependency analyses of the entire supply chain. Exhibit
10.4 illustrates a broad systems perspective of issues and
DOE’s Biomass Program employs a wide range of approaches for the overall algae biofuels supply chain from
analytical tools, data, and methodologies to support siting and resources to product end-use. The categories
decision-making, guide research, and demonstrate shown follow the process steps in the algal biofuel supply
progress toward goals. In conjunction with the Algae chain sequence. Such summarization of key areas to be
Roadmap, Biomass Program’s strategic and portfolio addressed to enable algal biofuel production provides
analysis efforts are expanding to include algae with guidance for the scope of content that should be integrated
techno-economic modeling and analysis (TEA), life cycle into systems techno-economic modeling and analysis.
assessment (LCA), and geographic information system
(GIS) based resource assessments.

Exhibit 10.2 Systems and Techno-Economic Modeling and Analysis as a decision-support tool
It can track and assess performance, and evaluate investment cost/benefit/risk tradeoffs to inform both technical and programmatic decisions

S&T, Systems & Processes Areas
Biofuel Conversion
Cost, Performance, and Risk Metrics

Extraction & Fractionation
Harvesting & Dewatering
Algae Science & Biomass Production
Stage-I Advances Systems Infrastructure & Integration
Uncertainty Range

Cost & Risk
Reductions Stage-II Advances
Improvements Commercialization Threshold

Cost & Risk

T0 (current status) T1 T2 (e.g., 5-15 years)

Roadmap Timeframe Multi-Stage R&D,
Demonstration, Commercializtion, Deployment

94 10. Systems and Techno-Economic Analysis 

Energy & Fuel Economics; Algal Biomass & Biofuel
Energy-Water-Environment Production Systems,
Interdepencies; Process Engineering
Carbon Emissions & & Other Sciences

Policy Analysis

Exhibit 10.3 Techno-Economic Modeling and
Analysis addresses interdependent issues

The scope of TEA will ideally include the capability to level, which in turn must function within climate and
apply and integrate detailed process engineering and weather conditions that vary with geographical location,
physics-based modeling at the unit operations level the approach needs to be both multi-level and multi-scale.
with multi-scale and multi-path systems modeling and
comparative tradeoff assessment. This can include An algae biofuels and co-products supply chain will be a
geospatial (GIS-based) data collection, analysis and complex interdependent system with numerous alternative
visualization, systems and processes optimization, pathways and functional elements and feedbacks at various
and dynamic interdependency analyses of the overall spatial and temporal scales and resolutions, as suggested
supply chain at a local, regional, national, or global in Exhibit 10.5. System and process simulation and
scale. A major environmental impact element will optimization under a system engineering framework can
be life cycle analysis (LCA) to assess GHG impacts prove very beneficial for system design and operation. For
and other resource use footprint (e.g., water). example, optimizing conditions that produce the highest
neutral lipid content of the algal biomass under a range
The overall goal is to capitalize on the power and insight of design conditions, when bio-oil feedstock production
available through application of well developed computer is the primary pathway and objective, may not lead to the
modeling and analysis tools combined with disparate highest overall biomass or lipid productivity. Similarly,
database information that exists or can be developed. achieving the most affordable and sustainable production
For example, a process-based algae growth model that of algal feedstock, in terms of resource use intensity
accurately reflects and integrates the dynamic behavior of and cost per unit of end-use biofuel produced under a
the key biological processes with the engineered growth specific set of scenario conditions, may not result from the
system and its time-dependent physical and chemical pursuit of the algal oil production pathway altogether.
conditions (including local time-varying climate and Sensitivity analyses and global system optimization
weather) can not only provide more detailed input to and comparative tradeoff assessments across a range of
the techno-economical performance analysis of algae approaches and conditions are among the critical modeling
cultivation, but can also reveal opportunities for improved and analysis needs. Meeting such needs requires a flexible
system and process design and operation. Since the modeling and analysis framework and suite of tools that
characteristics of the biological system at the microscopic can specifically address the broad scope and multiple
algae cell level affect the performance of the engineered pathways spanned by an algae biofuels supply chain
cultivation system and processes at a higher integrated system. Geospatial GIS-based data integration and analysis

10. Systems and Techno-Economic Analysis  95

Exhibit 10.4 Scoping the content for Systems Techno-Economic Modeling and Analysis: Key topic areas and issues for the overall algal
biofuels supply chain


Algal Biology Cultivation Harvesting & Extraction & Processes Biofuels Policy &
Sitting & Inputs
S&T Systems Dewatering Fractionation Co-Products, Regulation
& Services

Geolocation/ Species Selection Open Ponds Filtering Extract Processes Conversion Processes Taxes
elevation & Matching to - lined Flocculation/ - Solvent - Biochemical Incentives
Land characteristics Growth Conditions - unlined Settling - Acoustic - Thermochemical
- Electromagnetic - Digestion Permitting
Characterization - raceway
Climate/Weather Airlift Flocculation - Other - Hydrotreat/ Enviromental
- wastewater
Solar Insolation Performance treatment Centriuge Seperation/ Refine Impact
Water Souces/ Strain Improvement Closed PBRs Drying Fractionation Fuels Health & Safety
Issuses - horizontal tube Biological Assist - Membrane - Biodiesel Algae Control
- brackish Biomass Growth
- vertical tube - brine shrimp - Distillation - Green diesel & Regulation
- wastewater & Oil Conditions
- vertical planar - fish - Centrifuge - Aviation
- produced Photoautotrophic Other
- other - Other - Gasoline-like
- desalination organism operation Other
Hybrid System Intermediate - EtOH
concentration Products - Biogas/methane
Heterotrophic Combinations
-marine - TAG Oil - Other
organism operation - covered ponds
- fresh - Other Lipids Co-Products
Algae Pathogens, - pond/PBR mix
- losses, re-use ▪ Polar - Feed
- salt build-up Predators, and Heterotrophic ▪ Neutral - Fertilizer
CO2 Sources Mitigations - Industrial - Carbohydrates - Chemicals
- power plants Bioreactors - Proteins Services
- cement plants - Wastewater - Other - Carbon capture
- fermentation/ Facilities Compounds - Water treatment
other - Water
Chemical/Materials Monitoring & Direct secretion
Energy/Power Maintenance of EtOH or
hydrocarbon fuel
precursors into
growth medium,
avoiding Harvest &
Dewatering steps

is also key to siting and resource assessment and the design, uncertainty in data while still capturing the underlying
analysis and optimization of facilities location and supply dymanics of the overall system. Although quantitative
chain logistics (from algae cultivation through end-use uncertainty will remain, the results can provide qualitative
fuels and products biorefining, transport, and distribution). system behavior and trends still useful in helping
guide technical, economic, and policy decisions. More
Algal biofuels remain an emerging field at a relatively thorough analysis and modeling refinement, informed
immature stage of development. As a result, data for by field data expected to become available as larger-
detailed unit operations and process analyses for different scale pilot and demonstration projects are established
technical approaches for algae cultivation, harvesting, and and operated, will more clearly reveal the most critical
post-processing into fuels and other co-products is currently challenges and guide investments and technical advances
sparse and uncertain. Selected modeling and analysis needed to enable successful commercialization of
approaches can be used to effectively accommodate such economical, scalable, and sustainable algal biofuels.

96 10. Systems and Techno-Economic Analysis 

or enterprise level rather than broader efforts.Species .Performance . tilt.Solar Insolation Environment .Autotrophic . Policy & Regulation. etc.Capital Co-Gen . Systems and Techno-Economic Analysis  97 . and therefore. Operations. Replacement Market Externalities: Cost of Energy.Characteristics . Exhibit 10.Requirements Harvesting Dewatering Extraction Oil / Lipids . different levels within the broad systems scope presented. Maintenance. geology.Closed systems &/or Drying & Seperation . Workshop participants the role that systems TEA and LCA can and should play generally agreed that modeling and analysis should be a in support of algal biofuels development.Heterotrophic .Primary Energy Electric Power and Process Heat Generation Capital Construction. similar to the modeling approaches. Cost of Petroleum & Conventional Fuels. unit operation. Also identified critical part of a national algae biofuels research program was the need to adapt. One approach with photoautotrophic microalgae integrated systems modeling and analysis across the is modeling major cultivation system categories 10. their alternatives. soil) Broader .Water Water Capture . and utilize a range of and industry development effort. and levels of detail best suited to and analysis efforts underway by DOE in support of the perform the necessary modeling and analyses based on other bioenergy and biofuels programs.5 The dynamic coupling and interdependencies across the algal biofuels and co-products supply chain 10. scales. develop. Incentives & Barriers Siting . location. unavailable for broader use and objective assessment and validation. as exemplified by Exhibit 10. be considered in algae biofuels modeling and analysis production plant.6 illustrates various complementary modeling and scales will be defined in the early stages of the systems analysis approaches and techniques that come into play at modeling R&D effort and will evolve as time goes on. Included in the Workshop discussions was the question of Due to their more limited and immediate business how best to approach the multiple and disparate paths objectives.Labor . identified the need to clearly define the scope and determine and that the two should be coupled. Demand & Price for Co-Products vs. Details of systems’ and processes’ technical and economic performance achieved by industry and LCA Approach (Workshop other technology developers are also usually considered Results and Discussion) proprietary.Temperatures Design & Economy Feedbacks Nutrient .Materials Energy Feedbacks .Open systems . Monitoring.Climate/Weather Feedbacks Conversion Carbohydrates Biofuels Algae Cultivation . that should tend to focus on a technology or process. individualized modeling and analysis efforts and configurations of rapidly evolving systems and by industry and technology development organizations processes. Exhibit that the needs for coupled models of differing fidelity and 10.Equipment .Land (cost.Nutrients (NPK) or Treatment & Re-Use . It is also expected available and emerging information and data.CO2 / Flue Gas Conditioning Other Coproducts .GMOs Biology .Hybrid systems Feedbacks Resources Proteins & Conversion .7. It was noted that there Preparation for the Algae Roadmap Workshop and the is a need and role for both integrated systems modeling as discussion among participants during and after the event well as detailed engineering design and process modeling.3 Suggestions for TEA and supply chain.

. etc. Systems and Techno-Economic Analysis  . various groups doing ad-hoc modeling and assessment specifically focused on their chosen approach and Standardized interface requirements and definitions could technologies (e.6 Multiple levels and complementary approaches available for Algal Biofuels Systems and Processes Techno-Economic Modeling.. Etc. with learning curves & improvement projections . enviromental. transport. Beyond this.Technical.g. specific technologies. with time delays.ICARUS cost estimate software (or equivalent) • Operations & Maintenance cost estimates Geographic Information System (GIS) Analysis and Visualization • Cost (& offsets) of co-product feedstock • Land resources (characteristics. 98 10. • Other infrastructure and enviromental features Exhibit 10. economic. The size of the “matrix” engineering design. and policy decisions to best conceptualize a model that spans the overall at the state. closed photobioreactors. and optimization of of possibilities could quickly become unmanageable.) • Cost of biofuel production • Water resources (fresh wastewater. pipelines) Other LCA Modeling • Fuel processing.Integration with existing infrastructure .. from resources & sitting through production to end use • Algae biofuel and co-products industry scale-up potential. and activating only those portions of the issues and tradeoffs that need to be addressed together to hybrid configuration model desired.. and processes and performance parameters is another definitions. resource use. processes and integrated systems for commercialization. and policy issues • Feedbacks and Multiple Sector Interdependencies. production availability.. can link to other models & analyses Process Flow Enginering Modeling Static CAPEX & OPEX Calculations • System and process equipment cost estimates • Mass & energy balance calculations . and national level. Narrowly “beginning-to-end value chain” for algal biofuels focused and detailed technical and economic modeling production with adequate resolution and detail is very and analysis is valuable and necessary for those doing challenging and still emerging. storage • GREET (Argonne National Lab) infrastructure • LEM (UC/ Davis).. development.. and interface approaches. economic. GIS Analysis.. Flexibility and usefulness Developing numerous detailed models that are each could be increased to the extent that such modeling uniquely customized to a specific combination of systems and analysis can be done using common standards.. how better inform technical. across an emerging approach.Vendor/ Supplier cost estimates (ASPEN+) • Engineering and construction cost estimates • Process GHG footprint (LCA) assessment . The disadvantage of such a be established for system and process functional blocks that distributed and narrowly focused approach is that it is often would enable the development of an open-source modeling done in isolation with limited or no consideration of other and assessment platform with “plug & play” flexibility. • Cost (& offsets) of co-products production other) • Carbon footprint cost accounting • Climate/ Weather/ Temperature Conditions • CO2 resources ( point source emitters.Required build-up of new infrastructure. regional. platform). and essentially represents what exists today with “community of practice” for the algae biofuels industry.. or a hybrid competing options and the broader scope of interdependent combination. Higher-Level Dynamic Meta-Systems Modeling (Integrated Analysis Framework) • Broad value-chain scope. constraints & impacts . and Life Cycle Analysis of open pond.Engineering & construction estimates from industry .

2 Co-products 1. Proteins P. 2009).1 Chemical C.4 Off-shore systems 3. cultivation systems) or process engineering models using widely accepted and applied commercial process modeling tools like AspenPlusTM or customized spreadsheets.) 4.g.0 J. Dried Algal 1.1. processes.0 Harvesting L. 10. and national labs. EPA.7 is a revised adaptation systems. Chemicals 2.1 Closed system 1. and other technical Defense.1. Biogas / Methane Metabolites I. a process flow diagram was economic/policy R&D and assessment developed and presented at every breakout track discussion to illustrate the intricate interdependencies of algal • Industry developing and commercializing technologies.Storage 4.5 Biological Assist 2. Aviation 6.2 Fuel .2 Electromagnetic 5. etc.1 Flocculation 2. Energy Source derivative biomass & Markets 3. assessment.5 provides • DOE & national labs doing R&D.0 Extraction Conservation 6.2 Open system 2.3 Centrifuging biomass 3. biofuel production.3 CO2 2.4 BY PASS 6.2 Land Cultivation 3. and represents utilizing different techniques such as high performance an example of a GIS-based information gathering physics-based modeling (e. 5. etc. Biodiesel 5.3 Chemical G. Materials 2.3 Water 3.1 Fuel 5. USDA. Feed 1.) and non-technical issues. universities. Lipids H. moving in this direction (Getman. Green diesel 6. To facilitate system-level • Universities doing a wide range of technical/ thinking during the Workshop.Distribution .3 Hybrid systems & settling 1.1.4 Systems Analysis Flexibility in being able to link custom subsystem or process models into an overall meta-system Overview modeling and analysis framework would provide a System analysis is foundational to designing a strategy capability that could be of significant value and benefit for algal biofuel deployment. computational fluid dynamics and analysis platform that can be productively used modeling of open raceway ponds or closed photobioreactor and leveraged for algae biofuels development.Transport fish excrement.End Use Exhibit 10. Exhibit 10. Triglycerides N. 4. Gasoline-like 6.Field . and a high-level illustration of the interdependent character tracking of program investments of the overall algae biofuels value chain that involves a • Other federal and state agencies (Department of broad range of systems.1 Algae species 3. Other Q.2 Thermochemical & Separation D.3 Biochemical E. Fatty Acids M.1 Solar . processes of the flow diagram that shows a representative number • Private investment / funding sources 10. Systems and Techno-Economic Analysis  99 .5 Biological assist Harvesting (shrimp.1.0 1. A system is an aggregation to different stakeholder communities such as: of subsystems interacting such that the system is able to deliver an over-arching functionality.7 High-level multi-pathway algae biofuel process flow diagram for the algal biofuels and co-products supply chain Much more detailed or custom models of individual Work currently underway on the Knowledge Discovery subsystem or process blocks could then be developed by Framework (KDF) by DOE’s Biomass Program is various others in industry.1.4 BY PASS A.1 Physical (Thermo/Mechanical) 5. Ethanol 1. Hydrogen K..1 Microalgae Conservation F. Wet algal or biological assist Infrastructure 1.2 Filtering 8.2 Cyanobacteria R.5 Light or Alt.1. Fertilizer Inputs 1.0 Drying . Exhibit 10.3 Macroalgae R. Carbohydrates O.

(N. GIS Assessments sustainable production of microalgae biomass. The overall viability and examine the sensitivity of different options for hydrogen production include goal gasification. These Engineering analyses (technical and economic) at the include climate and the adequate availability of water. Though it may seem daunting to system component are shown. and understanding key information from each node in the and other local environmental conditions. among others. and each numbered node attempt to develop a comparative analysis based on so represents a mass or energy balance calculation. etc. Example engineering analysis tools scale-up was touched on earlier in chapter 9. P. Inputs to each will likely emerge. and tracking of mass and energy balances that include and availability of supplemental CO2 and other nutrient evaluation of the thermodynamic.8. of a given algal biomass production system and show 2009). there is precedence for this value of this kind of systems mass and energy balance sort of undertaking in DOE’s Hydrogen Analysis (H2A) assessment is that it can help assess the overall viability Project within the overall Hydrogen Program (DOE. Simpler and process becomes the primary task of the systems analysis. • Fuel processing. regional. The impact that availability and cost biological. number of permutations of potential pathways to algal biofuel production. evaluate costs. The cost include AspenPlusTM and FLUENTTM. availability. This program could serve as a guide for moving Geographic Information System (GIS) Visualization forward with analysis of algal biofuel production. and environmental impacts. identifying and appropriately modeling • Water evaporation loss (function of climate. Systems and Techno-Economic Analysis  .8 represents a mass and energy on the product or co-product chosen. The pathways toward a hydrogen economy. and Analysis tools (referenced also in chapter 9) are indispensible for algae production and supply Other chapters of this Roadmap point out the lack chain systems analysis due to their ability to perform of availability of detailed information about the mapping and resource analysis spanning local. etc. assumptions in individual processes to the overall system. The many process permutations.) enumerated and designated as goals to be achieved.of multiple path process options available for every step technical information and data about the systems and in the algal biofuel production chain. temperature. H2A was designed to consider various highlighting areas for research and development. etc. and chemical kinetics of the systems and of these resource can have on algae biofuel production processes used.8 represent a large detailed process engineering data may be lacking. and benefit tradeoffs of CO2 capture through biofixation which require sufficient understanding and detailed using photoautotrophic microalgae cultivation enhanced 100 10.) interdependencies and feedbacks. Sub-level accurate analyses. Exhibit 10. especially where Both Exhibit 10. such as: processes that are shown in the process flow diagram • Land and water resources of Exhibit 10. transport. nuclear energy. Collecting such as time-dependent solar insolation. development of mass and energy based systems models energy and environmental tradeoffs and set research can help evaluate different proposed processes for priorities and inform policy by sound analysis. storage infrastructure technical performance. and comparing trade. thus Fuel Initiative. Systems analysis can help manage the complexity of • Climatic characteristics (temperature. and organic molecule reforming. characteristics of algae themselves and the characteristics and national scales. risk. producing algal biofuels and co-products by quantifying precipitation. unit operations level require the systematic calculation efficiency of water use. most of which are still immature and An example of this type of analysis is presented in emerging. pipelines) offs from various scenarios with regard to cost. hydrodynamic.5 and Exhibit 10.8. A substantial number of barriers are (characteristics. from algae growth processes involved to appropriately formulate and conduct to fuel and co-product processing and end-use. and others beyond balance systems level view of an algal biomass and algal those selected for inclusion in the figures also exist or lipid cultivation and extraction system. wind electrolysis. This may also require coupling processes that made up different thematic topic discussion with appropriate external data and driving functions sessions in the Workshop are all inter-related. more approximate preliminary spreadsheet analyses are more appropriate as a first step. K) supplies. • CO2 resources (point source emitters. availability of suitable land. Critical climatic and natural (energy requirements and costs) of the systems and resource data can be readily accessed. • Other infrastructure and environmental features Engineering Analyses and Several critical resource factors will impact large-scale. solar insolation. Many of the process steps will differ depending Exhibit 10. Established in 2003 in response to the Hydrogen what steps in the process are most energy intensive.) uncertainties.

and Capture & Neutral Lipid Systems Infrastructure.9 is an example of the and costs of supplying inputs needed for algae production application of high-performance computational modeling (suitable siting. e. • Equipment. tools and capabilities to an open raceway pond system that supplemental CO2.. same would apply to co-products and their potential markets. this will include the logistics different site conditions. resource management. At the the technical design of systems and their operations for front end of the supply chain. sourcing. etc.g. storage.) forcing functions and boundary transport. nutrients.. with the existing hydrocarbon fuel handling. in press). and biorefining into fuels and (e.g. including example calculations presented later in this chapter. and data are also integral to system analysis as they reveal financial sets developed and leveraged to provide the necessary investment or market incentives needed for algae biofuel assessment capabilities to guide research.g.g. Biomass e. etc. These and related issues should be an integral Economic analysis tools for static capital expense part of techno-economic modeling. can help determine the practical upper limit • Cost (& offsets) of co-product feedstock production • Cost of biofuel production • Carbon footprint cost accounting Exhibit 10. storage. Systems and Techno-Economic Analysis  101 . Structures. for • Discounted cash flow analysis example. business deployment. e. Co-products Polar Lipid Site Preparation Carbohydrate 10. and policy decisions. and distribution into the various end-use conditions (James and Varun.. is discussed in more detail through a few selected The combination of GIS resource assessment. tools. Currently. the economics can be expected to improve. K. re-use Potrential Protein Biofuel Feedstock Materials. and transport of water. The possibility fuel and co-product markets at the end of the suppy chain.. from the operation and monitoring of larger scale algae distribution. and end-use infrastructure would result in production systems under a range of site and climate easier and more widespread market acceptance. This information. Hexane microfiltration e. the data available to implement this type of analysis are thought to be preliminary and refinement Producing biofuels that are highly compatible. or totally based on model improvement and validation with data fungible. the relative location and • ICARUS cost estimate software (or equivalent) logistical costs associated with fuel processing. Some examples are: development.g.. of combining these types of spatial and physical data to To the extent that operations can be effectively co-located project possible algal production scenarios as a function of or established within reasonable distances across the geography and climate is an interesting avenue of the addition of CO2 from industrial sources will affect on the size and the optimum site locations of biorefinery the economics of algal biofuel production. The conditions with multiple species of algae could be useful. with the appropriate models. and distribution infrastructure could benefit from GIS analysis. Midstream will be the includes an algae growth model driven by environmental logistics of transport. with physics-based modeling of will depend in large measure on the degree to which algae cultivation systems and algae growth. and energy). nutrient other co-products. ambient temperature. e. Primary Secondary Primary Secondary Neutral Lipid Bioreactor Harvesting Harvesting Dewatering Solvent Air & CO2 Dewatering Extraction &/ or Pond e. Exhibit 10.. geographic location attributes and time dependent climate The economics of algae-based biofuels and co-products and weather data. analysis and LCA (CAPEX ) and operational expense (OPEX) calculations for algae. solar insolation. Potential facilities relative to upstream algae biomass production CO2 capture and utilization in algae biomass production and downstream fuel and co-product markets.. can provide powerful feedstock production and downstream processing can be capabilities for estimating performance and for improving integrated with existing infrastructure and markets. supply chain.g. followed downstream by the necessary concentrations. Utilities.g. storage.8 Notional baseline algae feedstock production system Sunlight H2O Energy & Solvent & Water & O2 Other Inputs Energy Energy H2O Recycle Microalgae Photo. • POLYSYS At the downstream end.) Water Energy Residues: TAGs & Other Facilities. Land. Hexane System Chemical Dissolved Air Recovery & Centrifuge Drying Solvent Nutrients Flocculation Flotation (N. P. Equipment. Operation & Maintenance cost estimates transport.

700 gal/ac-yr. but these commercial algae industry build-up scenarios. • Availability.) (Weyer et al. Under large power. precipitation. availability.000 gal/ac-yr and perhaps a practical that can be captured. the productivity and reliability of production. etc. and mixotrophic) for which geographical in particular. productivity. photosynthetic efficiency. and fertilizer. 2009). These aspects were discussed in some detail in Chapter 9. Exhibit 10. and nutrient gradients • Solar insolation and other environmental. Computational Fluid Dynamics Model of Algal Raceway Pond Using Modified EPA and US Army Corps of Engineers Codes • The Environmental Fluid Dynamics Code (EFDC) solves 3D Navier-Stokes equations of open channel flow to model speed. and offshore sites. Open raceway pond example of physics-based algae cultivation system modeling and anlaysis. based of culture that will need to be processed on a daily on the expected losses. Areas The various inputs necessary for algal biofuel production of the country with the highest solar resource best have been described earlier in this chaper and in other suited for algae growth also tend to be more arid and chapters of the Roadmap. and this factor varies widely across the Sugar from commodity crops and other organic country among inland. water. and cost of organic carbon the following: feedstock needed as input for heterotrophic microalgae production will play a major role in the commercial • The average annual insolation is generally the viability and extent to which national production dominant and rate-limiting factor for autotrophic algal capacity can expand using the heterotrophic approach.350 . but it is appropriate here to briefly note • The supply. carbon materials from industrial or municipal waste This variation will determine the spatial surface streams can provide bridge feedstock in the near- 102 10. and meteorological forcing functions • Includes algal biomass growth model • CE-QUAL couples nutrient kinetics and 22 independent variables (N. seasonal. but will variation of resource availability will have major impacts be dependent on the geographical location. particularly with open systems subject to macroalgae and microalgae cultivation (autotrophic. vary over the U. and sustainability of suitable water Impact of Geographic Variability of supplies for algae production will be a key input factor Inputs on Algal Biofuel Production Costs for inland cultivation. and basis. can potentially help fill this need. Certain elements. it will affect the amount of CO2 the order of ~38. temperature. wind. approach the same order of magnitude as large scale There are. climate. 50% oil content. and annual variation in other assumptions made in the analysis (which include solar insolation. etc.9. P.5. like cost of subject to more limited water supplies. on the other hand. though important for overall TE analysis. coastal. are amount of water required nationally could begin to not unique to the development of algal biofuel technology. and will be heavily dependent on geographical location and local conditions.. aspects of large scale agriculture. The daily. heterotrophic.S. and it will affect the amount limit on the order of ~4. and affordable accessibility of such water sources. on cost of production and scale for commercial viability. the variations. Si. Capture and re-use of non-fresh water. cost. O2…) to model growth rates. as well as other climate-related the availability of high solar insolation consistent with factors such as temperature and weather (cloud lower latitudes and/or high percentage of clear weather cover. Systems and Techno-Economic Analysis  . in press) area of cultivation systems needed to achieve a set Recent analysis suggests an upper theoretical limit on amount of product. availability. evaporative loss. Such analysis can enable both performance prediction and pond system design optimization after appropriate refinement and validation with monitored field system data (Adapted from James and Varun.) will also affect both conditions.

and forest industries. Cost of site preparation and suitable for feeding heterotrophic microalgae. and petroleum). This As with cellulosic ethanol. carbon credits materials in the form of woody and herbaceous must also enter into this analysis. temperature control. Conversely. but major sustainable scale-up of national contaminants that inhibit algae growth). but applying facility will require extensive pipeline infrastructure for this heat or energy to extensive algal cultivation CO2 distribution. carbohydrates. N. 2009). For open nutrients for algae production scale-up. Waste heat and energy from will not be feasible in all instances and thus. It is reasonably (i. where recent cost and supply input resources with the required output product issues have had negative impacts on the industry processing and distribution infrastructure and markets. Mehta and Gaur. infrastructure facilities for offshore. the right of access and use. The affordability of • Land prices and availability can also impact the cost generating organic carbon feedstock from such of biofuel production at inland and coastal sites. 1998. the size of an autotrophic algae of suboptimal (high or low) temperature. P. lipids. the logistics and costs could impact the suitability of residual biomass for associated with producing. and and costs as transporting and distributing CO2. term. but evaporation will also add to • CO2 availability and cost of delivery will play a major operating cost (for water replacement and/or for salt role in autotrophic microalgae cultivation scalability management with brackish or saline water.. but this the cost of operations. Commercial fertilizer costs are overall cost of total algal biomass and intermediate tied closely to the cost of energy supplies (natural gas feedstock fraction (e. 2008). The capture and reuse of nutrients • As in traditional agriculture. and is a consideration that appropriately processing lignocellulosic biomass requires further investigation. Systems and Techno-Economic Analysis  103 .. on the industrial build-up. and operating expense. K) required as inputs for algae growth will straightforward to calculate the impact of the cost also play a role in commercial viability and extent of of land. adding to the cost. 2009) can potentially help supply specific strains for extended durations. derived originally from lignocellulosic biomass. and and processing improvements needed to reduce the the associated logistics. risks. the temperature during from various agricultural and municipal waste streams the growing season will restrict the ability to cultivate (Woertz et al. but for each approach operational costs for algae (Massingale et al. the amount of nutrients required nationally versus the matching of affordable production scale to could begin to approach the same order of magnitude the sustainable and affordable supply of the required as large scale agriculture. If it is agriculture (Huang. but this will be systems operating at inland sites in the summer. nitrogen than conventional biomass be possible to get the most benefit of economies of crops on a mass balance basis (Alexander et al.g. water evaporation rates will provide some level of and affordable accessibility of such nutrient sources. and can be an appreciable factor in proteins. and municipal waste streams will be location-dependent. and inland • The supply. and costs of offshore cost of lignocellulosic material deconstruction into marine operations will have a major impact on simple sugars and other organic carbon compounds costs of production. scale. and perhaps also for offshore sites. coastal. Finally. availability. and cost of other nutrients sites will all be location-dependent. Even in active thermal management for growth during periods the case of co-location.e. such as are present in some forms of flue gas. dependent on the geographical location. it will be advantageous to co-locate cultivation requiring active cooling that can add prohibitively to facilities with stationary industrial CO2 sources. As noted here and in chapter closed systems operating inland can overheat. 2009). transporting. In necessary to distribute the facility over a number of the absence of nitrogen fixation.. it may be co-located industries or the CO2 source may allow necessary to transport CO2 over some distance. 2005).. if not more. 2009. other) production. The quality of the systems will provide the same engineering problems CO2 source will also play a role for algal growth. (Huang. waste materials from agriculture clear how best to factor this into the calculation. Algae can be production capacity will demand the use of sugars effective at capturing and concentrating heavy metal and other suitable organic carbon source materials contaminants (Aksu. algae can require as smaller parcels of land or offshore sites. The key tradeoffs will be between the cost Under large commercial algae industry build-up of overall production (capital and operating costs) scenarios. some sources are likely to require more cleanup than others (especially if there are plans for animal feed as a co-product and/or if the CO2 source stream includes 10. materials will also depend on technical advances For offshore sites.. it may not much. and co-products like animal feed.. 9. 2008) it will likely be an optimum minimum and range to the same extent as for large scale commercial of size for a commercial production facility. availability. Malcolm et al. though it is not yet energy crops.

There are indications. the calculations for the cost of algal biofuel 10. algal oil feedstock Life Cycle Analysis production cost analyses were reviewed based on a Life Cycle Analysis (LCA) is a “cradle-to-grave” range of sources to gauge the status of algal biofuel analysis approach for assessing the resource use and technology. These included technical reports prepared environmental impacts and tradeoffs of industrial systems for the U. The process employs a and scenario studies. learning curves and improvement projections • Technical.5 Algae Production production will benefit from detailed inputs that take into consideration the variations in cost and availability of the Costs and Uncertainties essential elements for cultivation. and in comparison with fuels citable sources are quite dated. economic. 2001 and 2002). combination of improved biological productivity and fully integrated production systems can bring the cost The term “life cycle” refers to the major activities in the down to a point where algal biofuels can be competitive course of the product’s life-span. a general lack of demonstrated operating parameters The systems modeling and analysis framework for algae and widely varying basic assumptions on a number of biofuels must include the integration of appropriate parameters from algal productivity to capital depreciation LCA methodology and metrics (Curran. 1996). LCA is important for assessing relative Oswald. and specifically leverage past and current LCA knowledge base present a challenge in designing scaled work specific to biofuels (ANL 2009. While most algal biofuels production. use. process. and tools have been developed that include Argonne National • Algae biofuel and co-products industry scale-up Laboratory’s GREET “well-to-wheels” model (ANL.12 (ISO.. would be subject to narrow operating margins. 1997). and • Broad value-chain scope: from resources and interpretation as illustrated in Exhibit 10. 2009). peer-reviewed literature (e. EPA. that a and including algae (Kadam. environmental. LCA is a systematic technique to assess Systems dynamics modeling is a powerful and flexible the environmental aspects and potential impacts associated modeling approach that can foster collaborative analysis with a product. siting through production to end use LCA methodologies.In summary. A dynamics simulation model also phased approach that consists of four major components: provides an integrated analysis framework that can include: goal definition and scoping. waste stream 2002 and 2004). EPA. with petroleum at approximately $100 per barrel. variability in approach to final costs (from per gallon LCA is considered to be a key element of the scope of TE of algal oil to per kg of “raw” biomass) and illustrate modeling and analysis within the context of this roadmap.. and other resources. output flows. life cycle inventory analysis (LCI). and numerous others (EPA. EPA. 1987). 1997.. operating costs. 1993). LCA for biofuels can also • Integration with existing infrastructure assess impacts and tradeoffs associated with utilization • Required build-up of new infrastructure with time intensity for water. the UC/Davis Lifecycle Emission Model (Delucci. • Input resources. and policy issues • Feedbacks and Multiple Sector Interdependencies with links to other models and analyses 104 10. delays. including the raw material acquisition required to manufacture the product 10.11 illustrates the typical life cycle stages considered in an LCA and the typical inputs/ Dynamics Modeling outputs measured.g. Exhibit 10. and compiling suggested directions for research and development from the participants. resource use. eliciting discussion. Delucchi. 2004) up systems. To facilitate the Workshop’s can play a significant role in the national energy economy objectives of identifying needs. costs. and maintenance. they also present a wide based on other renewable and non-renewable feedstocks. Benemann and and processes. to final disposal. however. UNEP.g. potential. white papers / industry analyses. and co-product credits. 1996. In resource capture and reuse. shortcomings of the existing literature and modeling 2005). 2006. life cycle impact assessment (LCIA). or service. energy.6 Preliminary System (EPA. These 1993. modeling. it is system performance for an industry that has yet to be likely that a technology that will require the production commercially realized is one of the biggest challenges for of immense volumes of biofuel at affordable cost so it techno-economic analysis. from manufacture. 1997. Bernard. ISO. 2009. constraints and impacts 2009). Tapie and GHG emissions and other resource utilization (e. nutrients.g. co-generation addition to net GHG emissions. Systems and Techno-Economic Analysis  . While these variations Data gathering and validation of technical and economic may be minor relative to the technical uncertainties. energy) impacts among different approaches to personal communications with field experts. data base resources. and water. Department of Energy (e.S.

• Incorporate appropriate insights and benefit from alliances with industry associations 10. • Inform and perhaps guide formation and/or varying parameters values within pre-set ranges in order modifications to public policy to describe the uncertainty or robustness of model output. e. Systems and Techno-Economic Analysis  105 . Workshop effort and is currently a work in progress. Interactive graphical user interfaces. The model will eventually include • Identify external factors that will impact cost more detailed systems and processes modules for multiple • Provide plan for entry of algal biofuel into a renewable pathways being pursued for algae biofuels and co-products.The development of a system dynamics model for \in techno-economic analysis and the capture of data conducting scenario analysis of algal biofuels production from fielded systems as they emerge over time can scale-up and operations was initiated as part of the roadmap provided needed additional information. data and assumptions from the same information sources discussed earlier in this chapter and in chapter • Determine the current state of technology 9.11 Scope of Life Cycle Analysis (Adapted from EPA 1993) 10.can facilitate the • Identify critical path elements that offer opportunities use of the model to conduct rapid ‘what if’ analyses and for cost reduction tradeoff studies using different yield scenarios.7 Potential Directions The Techno-Economic Analysis can accomplish this by: for R&D Effort • Stressing dynamics over detail initially. and resource constraints. The model approaches1 currently includes only a limited amount of available data. ISBL and OSBL been developed further since the workshop. fuel portfolio along with the ability to do Monte Carlo simulation. technical • Identify research areas most in need of support and economic performance for various pathways. The preliminary participants specifically suggested that the following modeling uses algae production and resource availability areas be addressed in the modeling and analysis.g. and has • Employing modular modeling. continued progress INPUTS OUTPUTS Atmospheric Raw Materials Acquisition Emissions Waterborne Raw Materials Wastes Manufacturing Solid Wastes Use / Reuse / Maintenance Coproducts Energy Recycle / Waste Management Other Releases System Boundary Exhibit 10. • Establishing interface requirements between To adequately inform research and investment decisions sub-systems for algal biofuel deployment. expanding The model described above was initially prepared in detail later as available and needed outline form for the algae roadmap workshop.

Addressing the uncertainties in a systematic more rapid technological advancement leading toward and integrated modeling and analysis framework will help commercialization. monitoring. Goal Defination and Scope Interpretatation Inventory Impact Analysis Assessment Exhibit 10. 1997) • Leveraging university and industry resources algal biofuels industry. and evaluation of larger-scale algae biofuel production systems and processes that can provide data to validate and improve the modeling. have been identified along various steps in the value chain. operation.12 Phases of a Life Cycle Analysis (Adapted from ISO. significant algae-to-biofuels production system and fashion. A critical complementary need is • Maintaining industry standard notation. units. etc. can facilitate Roadmap. guide needed investments and speed the deployment of an 106 10. Throughout the roadmap workshop and report development When done in a closely coordinated spiral development process. the complementary bi-lateral feedback of process performance uncertainties (technical and economic) information and insight between the modeling and analysis. and the experimental and operational investigations These have been noted in this and earlier chapters of the with larger-scale systems in the field. Systems and Techno-Economic Analysis  . the development.

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in past efforts are discussed in the context of applicability to the algal biofuels challenge. but not necessarily producing transportation envisioned by the Council on Competitiveness (1996): biofuels from cultivated algal biomass at scale. increase the capital efficiency of commercial et al. thereby facilitating growth and development infancy. public- private partnerships have the potential to accelerate Since the 1980s. academia. given the current state of of a sustainable. and government programs aimed at public-private which can support a quickening pace of innovation. has increasingly invoked commercialization of algal biofuel technology. leading public-private partnerships not only for large-scale to rapid industry growth and a stable market. realizing benefits in both categories. the business strategies of many existing partnership most relevant to the advancement of the algal companies are focused on one or more aspects of biofuels industry is perhaps the research partnership algae. national laboratories and universities) for development of algal biofuels. 11.11. 2002).g. universities.. Partnerships are defined as cooperative arrangements engaging companies. in turn. This chapter discusses potential models for public-private and government agencies and laboratories in partnerships in general and specifically as related to algal various combinations to pool resources in pursuit biofuels.. 2006). Partnerships based participants. given benefit could pull together the current expertise and the fact that the algal biofuels industry is still in its facilities. The type of this industry. as well as reduces the risk of private 2001. academia can be either public or private. however.1 shows the potential benefits of for membership and intellectual property models. By focsuing these partnerships on pre- competitive research or critical problems shared by all Exhibit 11. Various models for such partnerships employed of a shared R&D objective.A 11. The the algal biofuels value chain. analyses of various federal agencies exposure to fundamental science and engineering R&D. the U.1 The Benefits of Algal Biofuels While benefiting both private and public entities from Public-Private Partnerships shared investment toward mutual objectives.. including specific studies on the impacts firms. investment. Indeed. and industry. This partnerships are documented (Audretsch et al. Link could. many of which may be investor-backed and of DOE programs on the clean energy sector (Brown. 2002. Public-Private Partnerships The Workshop participants emphasized the need The algal biofuels industry is evolving with numerous for DOE and other federal agencies to partner with players.. Industry benefits from public-private partnerships from the 1999). Public-Private Partnerships   109 . also noted the unique partnership on sharing of knowledge and capabilities for mutual environment in algal biofuels development.1 Benefits of algal biofuels public-private partnerships Public Benefits Shared Benefits Private Benefits Energy Security Innovation Quickens Technolgy Commercialization Long-term Clean Fuel Shared Risk & Industry Growth & Market Stability Capital Efficiency Solution Shared Investment Competitive Edge Climate Change Migration Domestic Energy Supply Breakdown Legal & Jobs Creation Sustainable Industry Regulatory Hurdles Clean Energy & Development Science Leadership Growth Stimulates Labor Pool A In this situation. 2001. Brown et al. More specifically. but also for research and technology developments of national interest (Stiglitz and Wallsten. algal biofuels industry.S. infrastructure projects. pre-revenue. collaboration between private entities (e..g. including characteristics Exhibit 11.. industry) and public entities (e. many focusing on one to a few elements in national laboratories. Gallagher et al.

of solicitations are system-oriented. By maintaining an active directory. This may result in the formation of teams of proposers 11. with the ultimate seminars can be important tools for creating a collaborative solution only as good as existing technology will allow. they are often formed to serve no other purpose. concept of collaborating on a common basis is the sharing of pre-competitive research results. new partnership. conferences. successful partnerships have have necessarily come together without a government- been identified to include the following attributes: funded solicitation. be proportional to the value of the contribution. prizes have been offered by various for the advancement of technology and know-how to organizations to support solving critical. Partnerships can bring together parties that have not organizations that promote a closed circle of partners who worked together before. these organizations provide for opportunity to facilitate deeper collaborations. Implicit in the gained through openness toward new partnerships. environment. where no single entity can address the complex research challenge. Partnerships are facilitated in than they can individually. Government solicitations are also an option to promote regulatory issues. However. These events are often 11. particularly in instances in which the team would not At the highest level. into a working system to solve a problem. such forums may favor mature • Consortia and trade organizations technology in the final stages of development where the • Team-oriented prizes focus can be on assembly into a working system. and provide publicity • Networking events such as seminars and conferences and marketing toolsets for competitors to attract future • Team-oriented government solicitations investors.C Prize forums can pull together developed technology 11. Through their structure.g. Basic research and early stage technology with limited funding Networking events such as workshops. which could both be a benefit have worked together for many years may not generate (new complementary capability) and a challenge (the the new thinking and fresh approaches that could be understanding of how to work together). these partnerships can • The partners collaborate on the basis of only be expected to last for the life of the project. Prize forums can also leverage reward money There are several forums for creating public-private from a variety of different funding sources (state and partnerships: federal government. or levels beyond the capability of any individual entity. and demand that the winning team meet or exceed certain success Private Partnerships criteria. and may compete poorly in this type of award. scientists and engineers can learn of new research. while increasing the opportunity to through public-private partnerships. labor).2 Components of Successful in a project-level partnership. However. which could allow In recent years. Through these events. organizations that may provide an opportunity for • The partners can achieve more through collaboration lasting partnerships. Public-Private Partnerships   . because some awards are based on having solved the problem. these environments through collaborations among • The benefits received from the partnership should the membership.B. Such solicitations have Public-Private Partnerships the potential to accelerate technology development.players (e. 2008). and private investors) into a single competition. technology scale-up and demonstration. consortia and trade organizations can also be an effective • The partnership should not openly conflict with the vehicle for researchers looking for a new approach or interest of other groups (Micheau.4 Partnership Challenges in the point at which new researchers can enter and where the Algal Biofuels Industry new thought or methodology is shared with the peers. global problems. and potential partners can explore areas of mutual interest. However. • The benefits of partnership outweigh the cost Consortia and trade organizations are membership of collaboration. Often these types develop and license technology with new partners. non-profit foundations. as well as a platform to share research.. including the development of biofuels. 110 11. national.3 Forums for Public. as common interest. industry would retain its collaborative research to tackle complex problems competitive edge.

etc. A compare some of these existing public-private consortia public-private partnership for pre-commercial-scale user models against these four external characteristics. government solicitations. lawyers. This type of partnership would greatly accelerate semiconductors.mitcep. it will need labor to run its are formed or sustained through networking operations. economic. all of the key attributes for successful partnerships noted earlier. nanotechnology. are needed to ensure that academia develops students capable of addressing the challenges industry faces As discussed in greater detail in this document. overcoming the lab-field and information technology.) or an open access repository and collaborate on the end goals (specific intended use. How inclusive is the membership and utilization to advance fuel conversion to its industry (or segment thereof)? technologies toward marketable products • Technology Commercialization.g. Also. construction personnel.Public-private partnerships—whether they As this industry expands. dewatering pathway.g.). particular algal • Share knowledge of existing algal strains through strain. or trade organizations—can be an be the workhorse that generates labor for this new industry. to road-test their technologies. The exhibit compares tested and scaled before full-scale deployment in the PPP models from five different industry sectors – field. Academia will competitions. 11. • Technology development in algal biology. several in algal biofuels. biofuels. develop new algae-based fuels and co-products. These include: are needed to engage field experts (e. collaborate on the development of dynamic and expected contributions of the membership. Algal biofuels will likely face similar issues since natural environmental constraints Different consortia models exist and it is possible to could have a significant impact on algal productivity.2. and the most difficult to implement. marketing • An open environment to share ideas and technology and public relations specialists. technologies developed in the • Industry Growth. facilities could allow for new technologies to be road- as illustrated in Exhibit 11. requirements.xprize. Is it structured to develop and commercialize new technology? In the biofuels industry. this characteristics. and fuel conversion. and academia. These consortia require • Labor force and intellectual talent to draw upon. algal growth and harvesting. Doing so may help define the boundary problem(s) for focus by a public-private consortium • Share knowledge of algal cultivation and and bring clarity to the composition. distributors) across the across the entire algal biofuels value chain. the established industry sectors represented by most B http://www. particularly between industry and public-policy and regulatory challenges. 2009). 11. These PPP models are scaling barrier and the lack of private capital to conduct mentioned only to serve as examples of the four external such applied research. aviation. in order to fully realize the potential key technological challenges must be addressed for of the algal biofuels industry. consortia. etc. real estate developers. monitoring of growth operations • Collaborate on the development of technologies Considering the following four external characteristics for biomass harvesting and extraction of can help evaluate consortia models: algal components from the biomass • Share knowledge of fuel requirements • Openness. collaborative consortia for technology development are • The generation of basic methods and C http://www. effective way to address technological. it is useful to conceptualize Technology partnerships could be used for following the various models for public-private consortia in terms objectives: of the five attributes of successful partnerships within the context of particular scenarios (e. public-private partnerships the algal biofuels to become viable.5 Modeling Partnerships for • Pre-commercial-scale user facilities that are Technology Development accessible to researchers and developers Of the different forums for public-private partnerships. Does it seek to grow the industry? laboratory have traditionally not translated well to the • Shared Investment. algal oil extraction. technology development.. Because the ecosystem in the algal type of partnership can help incentivize commercial biofuels industry is unique and differs greatly from investment of similar full-scale algal operations.. prize and innovate new cost-cutting measures. Does it share investment equitably? field (Willems. Collaborative partnerships. conversion process. value chain to address technical and non-technical needs. Therefore. performance aspects of development of new natural or GMO algal strains the fuel. By reducing scaling risks. Public-Private Partnerships   111 .

high-risk Agency • Partners with barriers through funding Research industry and • Industry partners to targeted R&D • State funding Centers universities commercialize developed IP • Industry growth • Financial and Biofuels (not a • Public conferences • IP licensed to interested supported through other resources separate legal and workshops parties with an evaluation education from national entity with of commercialization labs • Opportunities for potential • Limited industry employees) collaboration involvement to • Transfer of technology by address issues on publication industry growth 112 11. coordinated royalty-free license to industry roadmap to members focus R&D and spur • PPP can also license IP to on economic growth third parties • R&D conducted at Research • Address game. it could be helpful to consider these attributes and models. then adopted development • Now funded facilities amongst members • PPP owns created IP and • Develops solely by industry • Public conferences provides non-exclusive. • Government Government Centers or partner facilities changing.models presented. Public-Private Partnerships   . Exhibit 11. and determine how to best adapt / implement these attributes. discuss and debate the merits of each. However. this industry may find that no specific model will meet all of its needs.2 Comparison of Some Public-Private-Partnership (PPP) Models TECHNOLOGY SHARED INDUSTRY FOCUS ENTITY TYPE OPENNESS OF PPP INDUSTRY GROWTH COMMERCIALIZATION INVESTMENT • Collaborate on pre-competitive R&D • Designed selected by membership to increase competitiveness of • Transfer of technology by existing US firms in publication or member- marketplace • Open membership website data transfer • Provides • Cost-shared Non-Profit • Represent • Technology further commercialization by government Corporation industry interests developed to manufacturing network that and industry at Semiconductors (501c6) with • Forums inspiring solutions with external drives economic start-up physical cross collaboration partners. for an algal biofuels public-private consortium to meet its specific mission successfully.

academic • Pre-competitive research and national lab • Government selected by all members Non-Profit partners. • Industry gifts collaboration through Advisory legal entity free basis or exclusively. including years Aviation & FAA (not a industry. standardization operational. and regulatory designed to reduce bottlenecks operational costs Industry • IP licensed exclusively or and increase • Government consortium • Open membership. companies 11. • Technology • Founding universities physical technical. businesses of years employees exclusively to each other • Significant industry royalty-free involvement • Transfer of technology by provides market publication. royalty. • Industry growth funding for over 5 • Research conducted by Corporation • Technical through education years national labs and member Nanotechnology (501c6) with workshops. • No federal amongst committee to with employees royalty basis as needed to funding researchers from address issues on achieve the widest possible universities and industry growth dissemination. TECHNOLOGY SHARED INDUSTRY FOCUS ENTITY TYPE OPENNESS OF PPP INDUSTRY GROWTH COMMERCIALIZATION INVESTMENT • Open membership • Consortium of industry. academic as members agree to cross large established • Industry match separate legal and government license background and firms and small for same number entity with partners newly developed IP non. facilities business. Public-Private Partnerships   113 . and transfer focus and of knowledge through commercialization member-only database network • Donor-driven • Research conducted by model • Industry growth university • Industry and supported through University • Software is open source • State funding university partners education of California licensed over 4 years Information • Numerous forums • Limited industry Institute • Other IP is either licensed • University funds Technology Research inspiring cross involvement (not a separate non-exclusively. only licensed to member financial • PPP provides non-exclusive. non-exclusively general aviation funding for over 8 with NASA • Consortium of • Commercialization afforded market. and members commitment for paid-up license to industry social issues over 3 years members inspire cross collaboration amongst members • Collaborative research amongst consortium • Technology members designed development and to reduce technical.

1459-1466. 325(5941).N. Research Policy. K. 707-708. In Rosenau.. Short. Gallagher. 31(1).G. (2001).J.S. Public-Private Partnerships   . Willem. Link.. D.References Audretsch. 29(14)... P.S. A. (2001). and Sagar. Research Policy. Stiglitz. J. An analysis of public initiatives to promote strategic research partnerships.D. (2000). Brown..E. W. 29(14). 1197-1207.. Council on Competitiveness. (2002).P. Energy-technology innovation.B. Public-private policy partnerships. D.. S. (ed). (2009).V. 1179-1196. Scenarios for a clean energy future. The biofuels landscape through the lens of industrial chemistry. J. and Koomey J.D. and Wallsten. A. Holdren. Brown. and Siegel. A. Public-private technology partnerships: Promises and pitflass.A. Endless frontiers. Annual Review of Environment and Resources. Link. Science.A. limited resources. 31. 31. Market failures and barriers as a basis for clean energy policies. Energy Policy. The MIT Press. Levine. J. (2002). Energy Policy.S. M. M. (2006). and Scott. Public/private technology partnerships: evaluating SBIR-supported research. Paton. 145-158.. D. (1996). Washington: U. 193-237.T..A. P. R&D Policy for Competitiveness. M. 114 11.N.

Appendices Appendix 1 : Workshop Participants Walter Adey Smithsonian Institution John Aikens Lybradyn Mark Allen A2be Carbon Capture Gennady Ananyev Princeton Alex Aravanis Sapphire Energy Ed Archuleta El Paso Water Utilities Paul Argyropoulos Environmental Protection Agency Ricardo Arjona Abengoa Bioenergy Virginia Armbrust University of Washington Bob Avant Texas A&M Tom Avedisian U. Department of Agriculture Doug Borger Lockheed Martin John Bouterse Envo Smart Sara Boyd BCS. Department of Energy Biomass Program Leslie Bebout NASA Ames Research Center Lucie Bednarova General Motors Amha Belay Earthrise Nutritionals John Benemann Benemann Associates Linda Benjamin Food and Drug Administration Christoph Benning Michigan State Interdisciplinary Center Herzliya. Department of Energy David Bayless Ohio University Jacques Beaudry-Losique U.S. Lauder School of Government.S.S. Andrew Braff Wilson Sonsini Goodrich & Rosati Jim Brainard National Renewable Energy Laboratory Jerry Brand University of Texas at Austin Tom Brennan Arch Ventures Michael Briggs University of New Hampshire Jim Bristow Joint Genome Institute Kevin Brown - Malcolm Brown University of Texas at Austin Nathan Brown Federal Aviation Administration (Office of Environment And Energy) Kent Bryan City of Celina 115 Appendices   . Inc. Diplomacy Isaac Berzin And Strategy Catalino Blanche U.

S. Biomass Program Daniel Fishman BCS.William Buchan NASA Ames Research Center Jay Burns Bionavitas Arthur Butcher CPS Energy Thomas Byrne Byrne & Co Martin Carrera BP Matt Caspari Aurora Biofuels Chris Cassidy U. Inc. Todd French Mississippi State Christian Fritsen Desert Research Institute Matthew Frome Solozyme Maria Ghirardi National Renewable Energy Laboratory Appendices   116 . Department of Energy Biomass Program Michel Delafontaine Hawaii Biocrude Gregg Deluga General Electric Global Research Harrison Dillon Solazyme Dan Drell Office of Science Biological and Environmental Research Donn Dresselhuys Old Dominion University Bruce Eldridge University of Texas at Austin Tj Evens U.S.C.Lincoln Alice Chen Environmental Protection Agency Parag Chitnis National Science Foundation Daniel Cicero National Energy Technology Laboratory Mike Cleary National Renewable Energy Laboratory John Coleman Algenol Jim Cook Univenture Doris Cooksey CPS Energy Keith Cooksey Montana State University Michael Cooney University of Hawaii – Manoa David Daggett Boeing Al Darzins National Renewable Energy Laboratory K. Department of Agriculture Paul Falkowski Rutgers University John Ferrell U. Das University of Georgia John Datko Air Force Office of Scientific Research Chris Davies Independence Bioproducts Brian Davison Oak Ridge National Laboratory Mark Decot U.S. Department of Agriculture Heriberto Cerutti University of Nebraska .S. Department of Energy.

S. Department of Agriculture Mark Hildebrand Scripps Institute Masahito Hiratake Mitsubishi International Kent Hoekman Desert Research Institute Neil Hoffman U.S. Department of Energy. Golden Field Office Anders Handlos Grauslund Novozymes Zia Haq U. Department of Energy. Department of Agriculture Tom Gross If.S.S.Warren Gillette Federal Aviation Administration (Office of Environment And Energy) Jim Gillingham Valero Dan Ginosar Lawrence Livermore National Laboratory Robert Glass Lawrence Livermore National Laboratory Steven Gluck Dow Chemical Steve Gorin National Renewable Energy Laboratory Alison Goss Eng U. Biomass Program Michael Graves University of Massachusetts Bailey Green Lawrence Berkeley National Laboratory Eli Greenbaum Oak Ridge National Laboratory Nathanael Greene Natural Resources Defense Council Michael Gregoire U. Department of Energy Biomass Program Laurel Harmon UOP Deanna Hatch Organofuels Incorporated Pat Hatcher Old Dominion University David Hazlebeck General Atomics Bob Hebner University of Texas at Austin Grant Heffelfinger Sandia National Laboratories Erik Helm Environmental Protection Agency William Henley Oklahoma State Doug Henston Solix Biofuels Jurg Heuberger County of Imperial Kevin Hicks U. LLC Arthur Grossman Stanford Guritno “Guri” Roesijadi Pacific Northwest National Laboratory Molly Hames U.S. Department of Energy. Department of Agriculture Ed Hogan Natural Resources Canada John Hogan NASA Ames Research Center John Holladay Pacific Northwest National Laboratory 117 Appendices   . Biomass Program Suzanne Goss Jacksonville Electric Authority Paul Grabowski U.S.S.

Department of Energy Biomass Program Geetha Kothandaraman ConocoPhillips Walt Kozumbo Air Force Office of Scientific Research Heidi Kuehnle Kuehnle Agrosystems Peter Lammers New Mexico State University Cynthia Lane American Water Works Association Todd Lane Sandia National Laboratories Mario Larach Kai Bioenergy Corp.S. Inc Qiang Hu Arizona State University Neil Huber Army Michael Huesemann Pacific Northwest National Laboratory Peter Hug Recombinant Innovation.Andy Horne Natural Resources Development Jeff Houff Hydrogeologic. Miller Chevron Chevron Gil Jackson United States Agency for International Development Jacob Jacobson Idaho National Laboratory Robert Jagusch Minnesota Muncipal Utilitees Association Eric Jarvis National Renewable Energy Laboratory Jeff Jeter Honda Libby Jewett National Oceanic and Atmospheric Administration James “Jim” Sears A2be Carbon Capture Michael Johnson Massachusetts Institute of Technology Glen Kertz Valcent Alex Kinnier Khosla Ventures Douglas Kirkpatrick Defense Advanced Research Projects Agency Melissa Klembara U. Hunter-Cevera University of Maryland Biotechnology Institute Mark Huntley University of Hawaii Stephen J. Rachel Lattimore Arent Fox LLP Edward Laws University of Hawaii at Monoa Gordon Leblanc Petrosun Joo-Youp Lee University of Cincinnati Paul Lefebvre University of Minnesota James Lester Stratus Consulting Jack Lewnard Gas Technology Institute Casey Lippmeier Martek Kent Lohman Midwest Research Institute Tryg Lundquist California Polytechnic State University Appendices   118 . LLC J.C.

Department of Energy Lissa Morgenthaler-Jones Livefuels Jerry Morris Innovation/Biodomain . Mayfield Scripps Research Institute Mike Pacheco National Renewable Energy Laboratory Paul Panesgrau National Renewable Energy Laboratory Frank Parker Organofuels Incorporated Helen Parker Algaelink. Roberta Parry Environmental Protection Agency 119 Appendices   .John Magnuson Pacific Northwest National Laboratory Len Malczynski Sandia National Laboratories Mike Manella ADM Russell Martin Blue Source Anthony Martino Sandia National Laboratories Margaret McCormick Targeted Growth Patrick McGinn National Research Council John McGowen Biodesign Institute at Arizona State University Diane McKnight University of Colorado-Boulder Steve Meller Procter & Gamble Omar Mendoza United States Air Force Walter Messier Evolutionary Genomics Blaine Metting Pacific Northwest National Laboratory Anelia Milbrandt National Renewable Energy Laboratory Greg Mitchell Scripps Institution of Oceanography John Mizroch U. Department of Agriculture Alyson Myers Kegotank Biofuels Nick Nagle National Renewable Energy Laboratory David Nobles University of Texas at Austin Charley O’kelly Blue Marble Energy Lou Ogaard CEHMM Kelly Ogilvie Blue Marble Energy John Ohlrogge Michigan State University Jose Olivares Los Alamos National Laboratory Phil Ovitt Senator Brownback Office George Oyler Johns Hopkins University Stephen P.S. N.S. Kenneth Moss Environmental Protection Agency Chuck Mueller Sandia National Laboratories Jeff Muhs Utah State University Walter Mulbry U.V.Shell Global Solutions Inc.

S. Biomass Program Schild Philippe European Commission Philip Pienkos National Renewable Energy Laboratory Maria Piera Abengoa Bioenergy John Pierce Wilson Sonsini Goodrich & Rosati Daniel Pike AF Research Lab Jennifer Pinkerton LA Department of Water & Power Bradley Plantz University of Nebraska-Lincoln John Plaza John Plaza Robert Polak Phycal Juergen Polle Brooklyn College Matt Posewitz Colorado School of Mines Steve Potts EPA Office of Water Peter Pryfogle Idaho National Laboratory Steve Przesmitski National Renewable Energy Laboratory Theras Pugh American Public Power Association Ron Putt Auburn University Nigel Quinn Lawrence Berkeley National Laboratory Kenneth Reardon Colorado State University Hal Reed American Water Valerie Reed U. Department of Energy. Department of Energy. LLC John Robbins Exxonmobil Paul Roessler Synthetic Genomics Roger Ruan University of Minnesota Jim Ryan MOR Supercritical. LLC Gerson Santos-Leon Abengoa Bioenergy Richard Sayre Donald Danforth Plant Science Center Philippe Schild European Commission Gerald Seeley Old Dominion University/Company Mark Segal Environmental Protection Agency Mike Seibert National Renewable Energy Laboratory Joel Serface Kleiner Perkins Appendices   120 . Biomass Program Ron Reinsfelder Shell Global Solutions Bruce Resnick Cargill Curt Rich Van Ness Feldman Mark Riedy Andrews Kurth Kathy Roach MurphyTate.S.Ron Pate Sandia National Laboratories Leslie Pezzullo U.

Cai Steger Natural Resources Defense Council Dan Stepan Energy & Environmental Research Center Jake Stewart Organic Fuels Mark Stone Desert Research Institute Belinda Sturm Kansas University Transportation Research Institute Sidhu Sukh University of Dayton Xiaolei Sun Arizona Public Service Sam Tagore U.Lincoln Alan Weimer University of Colorado at Boulder Zhiyou Wen Virginia Tech Norman Whitton Sunrise Ridge Algae 121 Appendices   .S. Inc. Biomass Program John Spear Colorado School of Mines Karl Stahlkopf Hawaiian Electric Company. N. Department of Energy. Department of Energy.S. Biomass Program Evonne Tang National Academy of Sciences Jeff Tester Massachusetts Institute of Technology Robert Thomas South Australia R&D Institute Jonathan Trent NASA Ames Research Center Pat Unkefer Los Alamos James Van Etten University of Nebraska Peter Van Den Dorpel Algaelink. Evan Smith The Verno Group Seth Snyder Argonne National Laboratory Milton Sommerfeld Arizona State University Glenn Sonntag U.V. Inc.Pamela Serino Defense Energy Support Center Chris Shaddix Sandia National Laboratories Alex Shearer SRI International John Sheehan Sheehanboyce. Jean Vandergheynst UC Davis Tom Verry National Biodiesel Board Sanjay Wagle Vantagepoint Venture Kevin Wanttaja Salt River Project Michael Webber The University of Texas at Austin Alan Weber Marc-IV Consulting Donald Weeks University of Nebraska . LLC Tanja Shonkwiler Jamestown Ny (Outside Counsel) Blake Simmons Sandia National Laboratories Knut Simonsen DTE Energy Resources.

LLC. Department of Energy Golden Field Office Grant Heffelfinger Sandia National Laboratories Mark Hildebrand Scripps Institution of Oceanography John Hogan NASA Ames Research Center Qiang Hu Arizona State University Michael Huesemann Pacific Northwest National Laboratory Eric Jarvis National Renewable Energy Laboratory Geoff Klise Sandia National Laboratories Alina Kulikowski-Tan Carbon Capture Corp Pete Lammers New Mexico State University Jared Largen BCS. Inc. Appendices   122 . Molly Hames U. Maria Ghirardi National Renewable Energy Laboratory Stephen Gorin National Renewable Energy Laboratory Tom Gross IF.S.Elizabeth Willet Mars Symbioscience Bryan Willson Colorado State University Byard Wood Utah State University Christopher Wood Platt River Power Authority David Woodbury Biofuel Producers of America (Bpa Paul Woods Algenol Ben Wu Livefuels Don Wyma National Intelligence Officer For Science And Technology Joyce Yang U.S. Department of Energy Biomass Program Chris Yeager Savannah River National Laboratory Ross Youngs Univenture Tim Zenk Sapphire Energy Zhenqi Zhu Stevens Institute of Technology Yonathan Zohar University of Maryland Biotechnology Institute Appendix 2: Contributors Jim Brainard National Renewable Energy Laboratory Tom Brennan Arch Ventures Bill Buchan NASA Ames Research Center Mike Cleary National Renewable Energy Laboratory Michael Cooney University of Hawaii – Manoa Al Darzins National Renewable Energy Laboratory Daniel Fishman BCS. Inc.

Department of Energy. Department of Energy.S.S. Inc.Yantao Li Arizona State University Tryg Lundquist California Polytechnic State University Rajita Majumdar BCS. Department of Energy. Biomass Program Philip Pienkos National Renewable Energy Laboratory Juergen Polle Brooklyn College Matthew Posewitz Colorado School of Mines Ron Putt Auburn University Kathy Roach MurphyTate. Biomass Program Chuck Mueller Sandia National Laboratories Nick Nagle National Renewable Energy Laboratory Michael Pacheco National Renewable Energy Laboratory Ron Pate Sandia National Laboratory Leslie Pezzullo U. Len Malcynski Sandia National Laboratories Tony Martino National Renewable Energy Laboratory Steve Mayfield Scripps Institution of Oceanography Anelia Milbrandt National Renewable Energy Laboratory Joanne Morello U. LLC. Biomass Program 123 Appendices   . Guri Roesijadi Pacific Northwest National Laboratory Mike Seibert National Renewable Energy Laboratory Joel Serface Kleiner Perkins Chris Shaddix Sandia National Laboratories Blake Simmons Sandia National Laboratories Amy Sun Sandia National Laboratories Joyce Yang U.S.

Valentin Soloiu Georgia Southern University Lance Stokes ECI Environmental Compliance Jefferson Tester Cornell University Willem Vermaas Arizona State University. Inc. Stanford University Arthur Grossman (Adjunct Professor). Robert S Weber Sunrise Ridge Algae. Inc. Whitton. Bob Avant et al Texas AM Halil Berberoglu University of Texas Shulin Chen et al Washington University Barry Cohen National Algae Association Anju Dahiya 1) The University of Vermont . 2) General Systems Research LLC Matthew Frome Solazyme Michael R. Dr. LLC Carnegie Institution for Science (Staff member). Rittmann Arizona State University G. Gale PetroAlgae. Solazyme (Chief of Genetics) Douglas Haughey not listed Joel E. Biodesign Institute. Inc Matt Peak Prize Capital María Piera Alberola Abengoa Matthew Posewitz Colorado School of Mines Bruce Resnick Cargill Center for Environmental Biotechnology. Biotechnology Institute David L. Dr. Bruce E. Roesijadi Pacific Northwest National Laboratory Mary Rosenthal Algal Biomass Organization Richard Sayre and Ursula Goodenough Donald Danforth Plant Science Center and Washington University Gerard Seeley Jr.Appendix 3: Respondents to Request for Information on the Draft Roadmap Daniel Angell TPA Inc. Kostka Florida State University David Lewis The University of Adelaide Margaret McCormick Targeted Growth Lissa Morgenthaler-Jones LiveFuels. Wenbert The Eden Tube Project Norman M. Paul Woods Algenol Xiaohan Yang Oak Ridge National Laboratory Tim Zenk Sapphire Energy Appendices   124 . Algal Farms Inc. Mark Segal Environmental Protection Agency Office of Pollution Prevention and Toxics Alexander D. School of Life Sciences Marc von Keitz and Steve Heilmann University of Minnesota. Shenderov BioLogical Technologies.


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