A method used for detection of gunshot residue. This test determines whether a person has discharged a gun, the Paraffin-Diphenylamine test. Different names of this test: 1. Lunge’s test 2. Diphenylamine test 3. Dermal nitrate test 4. Gonzales test- the one who improved the test    Dr. Gonzalo Iturrios- first used paraffin for collecting gunpowder residues from discharged firearm in Cuba where the Paraffin test originated. The basis of this test is the presence of nitrates in the gunpowder residue. The test is done by applying warm melted paraffin wax on the hands of the alleged shooter and causes the pores of the skin to open and exude the particles of gunpowder residue. These particles are extracted by the paraffin cast (made of paraffin wax and absorbent cotton) and will appear as blue specks, when diphenylamine (DPA) reagent is added on the cast. The specks are generally located on the area of the thumb and forefingers. Time allotment for performing a paraffin cast: 72 hours (3 days) from the time of the alleged shooting incident. After the specific period, it will be impossible to extract the gunpowder nitrates. Nitrates from pores cannot be removed by ordinary washing of the skin and stay for 72 hours. That is why it is important to conduct the paraffin casting within the specific period. Characteristics of positive result for nitrates from gunpowder residue: “tailing deep blue specks”. Thus, nitrate from other sources like fertilizers, will give the same reaction of a blue color but without the “tailing”. Other substances similar to nitrate known as oxidizers will also react with the reagent in the same way.

Substance giving false positive result: 1. Fertilizer 2. Explosives 3. Tobacco 4. Urine 5. Certain cosmetics 6. Food samples 7. Cigarette  Paraffin test is NOT conclusive evidence but just corroborative evidence which intends to support the testimony of eyewitness/es. There are instances wherein the person who actually discharged a gun still gives a negative result because of several factors.

Factors for false negative result: 1. Type of caliber of ammunition 2. Use of gloves

3. Allow the wax to cool and solidify to form a cast before peeling them off. Wipe the hands of the subject with clean absorbent cotton. direct the subject to move the fingers first and slowly moving his hands out from the casts. technician who took the a white. Place the paraffin casts inside an envelope or a box for proper preservation. 6. translucent. Length of the barrel Age of the gun/ efficiency of mechanism Direction of firing Wind direction and velocity Humidity/ percentage moisture in air When 72 hours has already lapsed Paraffin wax . 2. Absorbent cotton Burner Tongs . 9. 4. Allows cooling for a few minutes so that it will not burn the skin. 4. time and date. Pour another layer of the wax until absorbent cotton has totally absorbed the wax. 8. Using a spoon or tong with cotton. 10. Let the subject place his hands on a clean sheet of bond paper with the palms facing downward and the fingers closed together. Heat the paraffin wax in a beaker/ casserole until it melts. odorless solid consisting of a mixture of solid hydrocarbons of high molecular weight. 7. Place another bond paper on top of the paraffin casts and staple together. witnesses and label the right and left hand. name of the subject. 6. 5. tasteless. pour the melted paraffin wax on the hands of the subject from the thumb and index fingers and the dorsal portion to the little finger side of the hands. Place on the bond paper the case number. 5. 7. 8. Let the subject wash hands with water without using any soap or detergent. When cooled. Cover the hands and fingers thoroughly with paraffin wax and place a thin layer of absorbent cotton to reinforce the wax. Paraffin Casting Materials and Apparatus: Paraffin wax Bond paper Beaker or casserole Procedure: 1.3.

PARAFFIN CASTING Data and Results Name:______________________________ Group:________ Course and Section:____________ Date:________________________ 1. What is the substance used to determine the result for presence of nitrates from gunpowder residue in the paraffin cast? What is the expected result for positive? 5. 4. Which hand did you use? 3. Were you able to make a paraffin cast? 2. Was it successful? How did you find the experience? Explain. What substances that gives a false positive result? How do they differ from the positive result? .