Course outline for Molecular Genetics (M 211A); for Medical, Dental and Pharm D Students.

(3) Credit Hours Course Coordinator: Dr. Nabil Bashir Course Faculty: 1. Dr. Nabil Bashir 2. Dr. Zeyad Akawi 3. Dr. Fayeg Migdadi Section Place 1 Science Hall 2 2 Nursing Hall 2 Time Day 9.45- 11.15 Monday, Wednesday 3.45-5.15 Monday, Wednesday

Jordan University of Science and Technology Faculty of Medicine Department of Biochemistry and Molecular Biology Fall 2011

Course Description and Objectives: Objectives of this course are designed to understand the basic principles of molecular Biology and Molecular Genetics. Emphasis will be given to those principles that have application in medical practice. The structure of DNA and RNA as genetic material, DNA organization and its replication, mutation and repair in both prokaryotes and eukaryotes will be covered. Furthermore, gene expression will also be discussed. Finally, the course will cover some aspects of cancer genetics, cytogenetics and molecular biology techniques. Molecular medical genetics is one of the most rapidly advancing fields of medicine and is now integral to all aspects of biomedical science. Every physician who practices in the 21st century will require a basic knowledge of the principles of human genetics and their application to a wide variety of clinical problems. The practice of modern medicine includes recognition of the role of genetic factors in health and disease. This requires knowledge of the structure, function, and transmission of genes and understanding of interactions both among genes, and between genes and the environment. The Department of Biochemistry and Molecular Biology has the responsibility for teaching a major part of the Molecular medical genetics curriculum at the University of Science and Technology. The following outline lists the objectives of the course material in Molecular medical genetics. -------------------------------------------------------------------------------Students in Molecular medical genetics at JUST should know and understand: 1. What genes are and how they are organized. 2. How genes are arranged in chromosomes and how chromosomes replicate. 3. How genes are transmitted from parent to child, how genes segregate, and the patterns of inheritance for dominant and recessive, autosomal and Xlinked traits. 4. The nature of mutations and how they are repaired, and how they contribute to human variability and disease.

5. What genes do: the flow of genetic information from DNA to RNA to protein. 6. How gene expression is controlled. 7. How polymorphisms, gene linkage, and human gene mapping are used in medicine. 8. The significance of the Human Genome Project to medicine. 9. The molecular basis of inherited disease. 10. The role of genetics in the pathogenesis of neoplasms and in the predisposition to malignancies. 11. The multifactorial nature of most human traits and the principles of multifactorial inheritance. 12. The clinical manifestations of the common chromosomal anomalies. 13. The concepts and clinical importance of genetic imprinting and uniparental disomy. 14. Common molecular and cytogenetic diagnostic techniques and how they are applied to genetic disorders. Recommended Book: 1. Biochemistry with Clinical Correlation, DEVLIN "7th edition". 2. Hand outs 3. Human molecular genetics, 4th edition, TOM STRACHAN AND ANDREW READ Lecture Number Introduction
1.

Topic

Chapter. Instructor Bashir Bashir Bashir Bashir Bashir Bashir Bashir Bashir Bashir Bashir Bashir

Nucleotides Structure & metabolism I: purines and pyrimidines metabolism Nucleotides Structure & metabolism II: purines and pyrimidines metabolism Structures of DNA ,Genome, Genes, Chromatin and Chromosomes) Structures of DNA ,Genome, Genes, Chromatin and Chromosomes)Chromosomes) DNA: Replication I DNA: Replication II Gene mutations, DNA repair , Gene mutations, DNA repair Transcription and RNA Processing I Transcription and RNA Processing I

20 20 2 2 4 4 4 4 5 5

2. 3. 4. 5. 6. 7. 8. 9. 10.

FIRST EXAM, 24/10/2011, 10.15-12.15, 8/12/2011

11. 12. 13. 14. 15. 16. 17.

Protein Synthesis: Translation I Protein Synthesis: Translation II Posttranslational Modifications I Posttranslational Modifications II Regulation of Gene Expression I Regulation of Gene Expression II

6 6 6 6 8 8

Migdadi Migdadi Migdadi Migdadi Migdadi Migdadi Migdadi Migdadi Migdadi Migdadi Akawi Akawi Akawi Akawi Akawi Akawi Akawi Akawi

Chromosomal structure and organization in the human HO cell 18. DNA Recombination 4
19. 20.

Chromosomal numerical variations Chromosomal structural variations

HO HO HO HO HO 25 25

SECOND EXAM, 8/12/2011, 10.15-12.15 21. Principles of inheritance I (Mendelian inheritance I)

22. 23. 24. 25. 26.

Principles of inheritance II (Mendelian inheritance II) Non-Mendelian inheritance (Gene imprinting) The molecular genetics of Cancer Cell I The molecular genetics of Cancer Cell II

polymorphisms, gene linkage, and human gene mappingHO in medicine. 27. Molecular diagnosis of genetic diseases I 7
28.

Molecular diagnosis of genetic diseases II

Exams: Times and Dates will be announced later Exam 1 (30%): includes Lecture 1 to 10, FIRST EXAM, 24/10/2011, 10.15-12.15, Exam 2 (30%): includes lectures 11 to 20, SECOND EXAM, 8/12/2011, 10.15-12.15 Final Exam (40%): Comprehensive