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GENERAL LH (Luteinizing Hormone) is one kind of glycoprotein hormone. Just like FSH (FollicleStimulating Hormone), it’s one of the main factors motivating the ovulation. In female mammals, LH is cooperating with FSH to aid the follicle growing maturely and motivate the ovulation. In addition, it enhances the blood flow. Ovulation is the process in which the ovary releasing the ovum and it’s the key event in ovary period. The ovulation of mammals is a complex process involved with many genes and steps. A lot of research indicates that ovulation is regulated by hormone and partial active substance of follicle. Several or more hours before the ovulation of mammals, secretion of LH would rise in response to female hormone positive regulation and LH surge appears. After the LH surge, the wall of the enlarged follicle ruptures at ovulation and the mature ovum is extruded. After ovulation, LH returns to its base line level with the concomitant increase of progesterone level to initiate luteal phase. Before ovulation, the LH in urine, blood serum and milk rises gradually and the ovulation occurs approximately 12-14 hours after the LH surge. In view of the characteristic variation of LH, rapid and sensitive measurement of LH is an important tool in the diagnosis and management of infertility in female mammals. HOW TO USE As the cow come into heat, perform this test after ten hours for normal cows which shows heat in proper cycle. If found positive, breed that cow with interval of 12 hours. Incase of negative result, perform this test again with the interval of 5 to 6 hours and breed at positive result. For disturbed heat cycle cows, perform this test after 12 to 14 hours later to start of heat. If found positive, breed that cow with interval of 12 hours. Incase of negative result, perform this test again with the interval of 5 to 6 hours depending upon the heat cycle history of cow. It is important that cow should be breed twelve hours later to positive result. SPECIMEN COLLECTION AND PREPARATION Take the urine sample as it is as or take blood, centrifuge it and separate blood serum as sample for this test. ASSAY PROCEDURE 1. Remove the test device and dropper from its foil wrapper by tearing along the slice. 2. Using the dropper, withdraw the urine or serum sample from the specimen cup and slowly dispense 3-4 drops into the circular sample well. When the sample liquid moves slowly to the other end due to the capillarity, it will dissolve the solid gold labeled antibody and brought it ahead in the mean time. 3. Read results within 10 minutes. INTERPRETATION OF RESULTS Positive: If the test band (T) is more intensive than or equivalent to that of control band (C), the specimen is likely at surge level. Negative: If only the control band (C) appears, or the color intensity of the Test line is less than that of Control line, this means the specimen is below to the surge level. Invalid: When neither test nor control band appears on the membrane, the test should be voided since improper test procedure or deterioration of reagents probably occurred. It is recommended that the test be repeated. PRECAUTION 1. Read directions for use carefully before performing this test. 2. Do not uses test kit beyond expiration date. 3. Do not reuse the test devices and discard it into the dustbin after single use. 4. Once opened, the test device should be used immediately. Prolonged exposure to atmosphere will lead to deterioration.

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