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Azimah, H., Ahmad, S.H. , Nurfatin, M.R. Crop Science Department, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.

E-mail: Keywords: phytosanitary requirement, peel, pulp, flavor Abstract An experiment was conducted to evaluate the effect of storage duration after methyl bromide treatment on the durian postharvest quality characteristics of durian. Since the plant quarantine regulations for certain country are quite strict, the market demand of tropical fruits for exported to overseas especially China is abundant and competitive. Thus, there are certain treatments that are required to apply on the produce before exported. Durians were treated with methyl bromide in this study, which has been widely used for plant quarantine purposes. After that, durians were brought to postharvest lab, Universiti Putra Malaysia for further analysis. Optimum temperature during cold storage for durian is 13 to 15°C in 7 to 14 days of storage with 90 to 95% relative humidity. In this study, durians were stored at 13°C. There were changes during storage in L*, C* and h° value of peel, C* value of pulp colour and flavor hedonic evaluation. Peel colour and flavor of durian D24 changed during storage at 13 °C, following methyl bromide fumigation. These changes affect both appearance and palatability of the fruit. Further studies need to be carried out to determine suitable storage temperature for durian after fumigation with methyl bromide.

INTRODUCTION Durian (Durio zhibethinus Murray) belongs to the family Bombaceae and is widely grown in tropical and sub-tropical zones. It is well-liked and recognized for its flavor, aroma and texture. The durian tree, native to Southeast Asia, is very tall and the durian fruit is roughly the size and shape of a spiky football. Inside the tough skin are five white segments enclosing two or three portions of soft cream-coloured flesh, each wrapped around a single large beige seed. Thailand has dominated the durian trade for more than three decades, with nearly 60 percent of global durian exports coming from Thailand (Nanthachai, 1994).The breakthrough for Malaysia's durian growers was after the Chinese Premier, Wen Jiabao, visited Kuala Lumpur in April 2011, when he formally agreed to allow the entry of Malaysian durians into China.The Chinese tourist insisted that Malaysian durians are deliciously creamier and more aromatic than Thai-grown ones. The Malaysia's Federal Agriculture Marketing Authority reported that the country produces about 330,000 tons of durians a year, mainly for domestic consumption (2011).

UPM and stored at 13 °C for 10 days at 0. with 1 = hardly perceptible 10 = full durian flavor (Shashi and Khurdiya. The dosage used was 32 g/m3 of methyl Bromide (MB) at a temperature of 23 °C for 2 hours. Faculty of Agriculture. promising that demand from China would help prevent any potential excess in the domestic market. C* (chroma) and h° (hue) values. the duration between harvest and until the durian reaches the market often takes a longer time and unsuitable storage temperature during transportation for export will cause rapid ripening and reduce fruit flavor. 2004). Peel colour change appears to be a reliable indicator of ripening and quality in various fruits. L represent the brightness measure at a range from 0 to 100 ( 100 = white. was used. Therefore. Flavor (hedonic score): Pulp flavor sensory evaluation of ripe durian was evaluated by a panel of 10 semi-trained panelists which comprised postgraduate students from the Crop Science department.Nevertheless. Peel and pulp colour: Colour was determined by using a chromameter which provided L* (lightness). Malaysian officials have urged farmers to boost production.As a phytosanitary requirement for export to China. C* and h°) and flavor. RESULTS AND DISCUSSION L*. objective of this study was to determine the effect of storage duration after methyl bromide treatment on quality characteristics of durian in peel and pulp colour (L*. 2 and 4 days. with 10 representing the most favorable response. Faculty of Agriculture. Optimum temperature during cold storage for durian is 13 to 15°C in 7 to 14 days of storage with 90 to 95% relative humidity. Data were analyzed by using analysis of variance and regression analysis to describe the relationship between peel and pulp colour and flavour sensory evaluation during storage after methyl bromide fumigation. the durian were taken to the Postharvest Lab. 0 = black ) and C* measured the vividness of colour. Universiti Putra Malaysia. A continuous scoring method consisting of a 1-10 cm line. The projected export of frozen durians from Malaysia to China is between 350 to 500 tons a year for the year 2012 to 2015. the durian fruits will have to be fumigated with methyl bromide to kill fruit flies’ lava and eggs disinfestations. C* and h° value of durian peel and pulp . However. MATERIALS AND METHODOLOGY Durian cultivar ‘D24’ was harvested and fumigated with methyl bromide following Malaysian Fumigation Accreditation Scheme (AFAS) standards. h° is the qualitative attribute of colour and it defines the difference of a colour with reference to grey. Experimental Design The design of the experiment was a randomized complete block design. Then. with three replications.

18 % when pulp C* value of durian were measured between 0 and 4 days after methyl bromide fumigation.05) negative linear relationships with storage days after methyl bromide fumigation. to asses the commercial value of the product. The appearance of the fruit greatly influences consumer acceptance (Goldschmidt et al.There was a significant (P≤0.. there were no significant (P≤0. responsible for green. (2006) and Ketsa and Pangkool (1995).05) relationships between pulp L* and h° and storage days (Figure 1 (d) and (f)). Colour is a function of the light striking the product. where the pulp colour of durian changes from light to dark yellow upon ripening depending on the cultivar due o the increase synthesis of B-carotene. L* and h° values of durian pulp colour showed an increase during storage days. In this study. between 0 and 4 storage days.9% between 0 and 4 storage days after methyl bromide fumigation. Both C* value and h° showed a decrease of 51. However. Singh and Reddy. the differential reflection of certain wavelengths.46%. in some fruits accumulation of anthocyanin. However. with only changes in its chromaticity during storage. This could be due to the different cultivar showing different colour changes in the pulp. Black is always a ripe fruit whereas red fruit are not necessarily at optimum maturity for consumption (Steyn. As the durian ripens. colour in fruits and vegetables are mainly due to three families of pigments. 2005). chlorophylls. many quality control protocols use colour as an attribute to measure the degree of quality of fruits. Both peel C* and h° in Figure 1(b) and (c) showed there were significant (P≤0. Figure 1 (e) showed that there was a decrease of 14.05) positive linear relationship between peel L* and storage days after methyl bromide fumigation. (2002). 2009). Sensory flavor . These results indicated that the pulp colour remained yellow. Thus.8% and 39. 1977. 1999).05) negative linear relationship between pulp C* and storage days after methyl bromide fumigation.. there were significant linear relationship between pulp C* and storage days after methyl bromide fumigation. This was supported by Añabesa et al. Figure 1 (a) showed that L* value increased by 12. Peel colour changes due to preferential chlorophyll degradation appears to be a reliable indicator of ripening in various fruits (Wills et al. However. which are perceived due to the presence of pigments within products (Kays. and the visual perception of those wavelengths. 2007). carotenoids and anthocyanins. and foods in general. but there were no significant relationships between the variables studied. According to Artés et al. red-yellow and red to blue-purple colours. respectively. betalains and carotenoids gives rise to the characteristics colours of ripe fruits. peel colour changes from green to brownish-green. There was a significant (P≤0. respectively.

L. agrichemicals and nutrition will also influence the flavor. Technol. Goldschmidt. & Fellman. (1956). In Colour in Fruit Improving Quality. Kays. D..P. R. and smooth texture. Analysing changes in fruit pigments. (1999). (2001) reported that sugar and acid have the opposite effect on flavor and that the tongue is more sensitive to acidity. Riov. Further studies need to be carried out to determine suitable storage temperature for durian after fumigation with methyl bromide REFERENCES Artés.. Monselius.75% and 16. besides genetics. Seelye. 55 (6). Plant Physiol. Cambridge England: Woodhead Publishing Ltd. Preharvest factor affecting appearance of fruits and vegetables.J.Lost of durian flavor and texture started on the second day after fruit drop (Voon et al... methyl butanoate. Peel colour is closely related to the flavor of the fruit. ethyl-3-methylbutanoate. Y. bitter and overall after taste.Figure 2 showed there was a significant (P≤0.E. Differential counteraction of ethylene effects by gibberellins A3 and N6-benzyladenine in senescing citrus peel. and D. cultural practices. Sensory attributes generated to describe durian included sweet. 36–44. although these compounds do not correlate directly with the intensity sulphur notes perceived. J. Mattheis.05) positive linear relationship between sensory flavor and storage days after methyl bromide fumigation. M. Commodity tolerance studies of deciduous fruits to moist heat and fumigants. together with sweet.. According to Mattheis and Fellman (1999). 1977. 24: 297-355. This means that flavor rating decreased each day during the 4 days of storage. compared to at 0 storage day (control) after methyl bromide fumigation. H.Postharvest Biol.M. Preharvest factors influencing flavour of fresh fruit and vegetables. & Vines. Food Technol. McGlone. E.38% after 2 and 4 storage days. Sensory descriptive analysis indicated that sulfur compounds were involved in the typical. S. R.I. 1999.B. 2002. creamy. A. propyl-2-methyl propanoate. CONCLUSION Peel colour and flavor of durian D24 changed during storage at 13 °C. Mínguez. Jordan et al. J. S. and CRC Press. 2007).. M.K. alcohol. L. following methyl bromide fumigation... environmental. sticky. respectively. 15:233-247. pp 248-282. nutty and green aroma. J. 227–232. and S. . Claypool. Hornero. sulphur. MacDougall. Several compounds. Eilati. 2001. Postharvest Biology and Biotechnology. Aharoni. 59:193-195. 15. basic durian flavour. A sensory-based alternative to brix/acid ratio. These changes affect both appearance and palatability of the fruit. F. like ethyl propanoate. Hildardia.. K. This indicated that flavor was reduced linearly as the storage days increased. Jordan. fruity. ed. The flavor showed a decrease of 8. were highly correlated with the sweet and fruity notes of different durian cultivars.

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76. y = 27. y = 34.82 + 1.c) f) Figures a) d) b) e) Figure 1.59x. R2= 0.62x. b) peel C*.03 x. d) pulp L*.1. Solid line indicates a significant linear response. e) pulp C*. R2= 0.14 . R2= 0.38 . .21x.63. y = 12.27 .8. Relationships between a) peel L*. R2= 0. y = 87.85. c) peel h°. f) pulp h° and storage days after methyl bromide fumigation.1.22.

0.62) and storage days after methyl bromide fumigation. .Figure 2. y = 8. Relationship between sensory flavor.06 .33x (R2= 0. Solid line indicates a significant linear response.