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Name of the candidate: Abhinav.J Name of the instructor: Mrs.

Bhanu Date: August 2, 2011 Research question: What is the effect of concentration of salt solution on plasmolysed cells of Rheodecolorised leaves? Hypothesis: The no. of plasmolysed cells increases as the percentage of the salt solution increases. INDEPENDENT VARIABLE: Concentration of NaCl solution 1%, 2%, 3%, 4% and 5%. DEPENDENT VARIABLE: No. of cells plasmolysed CONTROL VARIABLES: Time: The leaf was dipped in NaCl for 1minute Volume of solution: 10 ml Temperature: Room temperature i.e. 27C.

Apparatus required: S.No. 1. 2. 3. 4. 5. 6. Materials required Microscope Leaf NACL solution Petri dish Microscope slide Brush Quantity 1 2-3 10ml 2 1 1

Procedure:

1.) 2.) 3.) 4.)

Rheo Decolorized leaves were taken and the peel was taken out. The peel was kept in distilled water for 1 minute. Then the peel was transferred through the brush to the slide. The peel was viewed under the microscope and the numbers of cells present were counted. 5.) Another peel was taken and kept in the petridish with a salt solution it. 6.) The peel was kept in the solution for about 1 minute. 7.) With the help of forceps the peel was kept under the slide. 8.) On the slide the cover slip was placed and it was placed in a such way that they were no air bubbles 9.) The slide was placed on the microscope and was viewed and the number of cells plasmolysed was counted and noted down. 10.) The process was repeated with other solutions as well and the readings were taken down.

RAW DATA: Percentage of the solutions 1% 2% 3% 4% 10% Calculation: To find the percentage of plasmolysed cells: For example: Percentage of the solution=1% No. of cells plasmolysed=21 Total no. cells plasmolysed=162 So the, percentage of plasmolysed cells=13.0% No. of cells plasmolysed 21 23 23 24 66

Processed Data: Total number of cells=162 Percentage of the solutions 1% 2% 3% 4% 10% No. of cells plasmolysed 21 23 23 24 66 Percentage of plasmolysed cells 13.0% 14.2% 14.2% 14.8% 40.7%

Graph:

Observation: 1.) It was observed that if the percentage of the solution increases the number of the cells plasmolysed increases so it is directly proportional. 2.) When the leaf peel was observed normally under the microscope it was in purple in colour. 3.) When the peel was observed after taking out from the salt solution few of the cells were shrunken and were green in colour. 4.) The shrunken and green colour indicates that the cells are plasmolysed. Conclusion: As the concentration of the cells increases the number of plasmolysed cells increases so the above stated hypothesis is proven. Precautions: 1.) While peeling the leaf it should be done carefully and if required a blade can also be used. 2.) While keeping it in the salt solution it should not exceed a minute or more. 3.) The peel should be transferred through using a brush.

4.) It should be viewed under microscope only in proper conditions with proper ventilation. Improvements: 1.) A well advanced and better microscope could be used for better results. 2.) A blade could be used instead of using hands peel the leaf. 3.) Proper ventilation should be provided.