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Glucose

Physical Properties:     Colour : Colourless Odour : Odourless Appearance : Clear Reaction : Neutral Observation No change.(Neutral) No appearance of violet colour. Appearance of violet ring at the junction of two solution. No appearance of blue colour. Appearance of red precipitate. Appearance of red precipitate. Appearance of red precipitate. No appearance of red colour. Result The solution is not Casein. It is not Protein. It is CHO.

Test Litmus paper Biuret Test Molish Test

Iodine Test Fehling Test Benedict Test Barfoid Test Selliwanoff Test

It is not Starch. It is reducing sugar. It is reducing sugar. It is reducing monosaccharide. It is not Keto Sugar.

Conclusion: The unknown solution is Glucose.

It is keto sugar. Reaction : Neutral. No appearance of blue colour. It is reducing sugar. Appearance of red precipitate. It is reducing monosaccharide. Aspect : Clear. . It is reducing sugar. Conclusion: The unknown solution is fructose.(Neutral) No appearance of violet colour. Result The solution is not Casein. Odour : Odourless. Appearance of red colour. Test Litmus paper Biuret Test Molish Test Iodine Test Fehling Test Benedict Test Barfoid Test Selliwanoff Test Observation No change. Appearance of violet ring at the junction of two solution. It is CHO. It is not Starch. It is not Protein. Appearance of red precipitate.Fructose Physical Properties:     Colour : Colourless. Appearance of red precipitate.

Aspect : Turbid. Appearance of red It is reducing precipitate.(Neutral) Result The solution is not Casein. ring at the junction of two solution.Starch Physical Properties:     Colour : Whitish. . Appearance of red It is reducing precipitate. Test Litmus paper Biuret Test Molish Test Iodine Test Fehling Test Benedict Test Acid hydrolysis:  Benedict & Fehling after neutralization  Iodine Test No appearance of violet colour. Appearance of blue colour. Complete Appearance of red hydrolysis of precipitate. No appearance of blue colour. It is Starch. Conclusion : The unknown solution is Starch. sugar. Observation No change. Odour : Odourless. Reaction : Neutral. Appearance of violet It is Carbohydrate. It is not Protein. starch to glucose. sugar.

 Selliwanoff Test No appearance of violet colour. sugar. Odour : Odourless. blue colour. Observation No change. . No appearance of It is not Starch.(Neutral) Result The solution is not Casein. Presence of Appearance of red reducing sugar precipitate.Sucrose Physical Properties:     Colour : Colourless. No appearance of red It is not reducing precipitate. Reaction : Neutral. Aspect : Clear. Test Litmus paper Biuret Test Molish Test Iodine Test Fehling Test Benedict Test Acid hydrolysis:  Benedict after neutralization. Appearance of violet It is Carbohydrate. sugar. Appearance of red colour. ring at the junction of two solution. (glucose). Conclusion : The unknown solution is Sucrose. Presence of ketone sugar (fructose). It is not Protein. No appearance of red It is not reducing precipitate.

Lactose Physical Properties:     Colour : Colourless. Reaction : Neutral. Observation No change. blue colour. It is not reducing No appearanace of monosaccharide. ring at the junction of two solution. Conclusion : The unknown solution is Lactose.(Neutral) Result The solution is not Casein. Appearance of violet It is Carbohydrate. It is not Protein. red precipitate. Odour : Odourless. Aspect : Clear. Appearance of red It is reducing precipitate. sugar. Test Litmus paper Biuret Test Molish Test Iodine Test Fehling Test Benedict Test Barfoid Test No appearance of violet colour. . No appearance of It is not Starch. Appearance of red It is reducing precipitate. sugar.

ring between two solution. Protein containing Appearance of purple tryptophane. Reaction : Alkaline. Appearance of violet colour. It is not Albumin. Aspect : Clear. containing sulphur containing amino acid. . to orange by adding ammonium hydroxide. Observation Red litmus paper turn to blue (Alkaline). Protein containing White precipitate phenol ring. Result The solution is Casein. Odour : Odourless. Test Litmus paper Biuret Test Heat Coagulation Test Colour Reaction Test: (a) Xanthoprotein Test (b) Millon Test (c) Rosenheim Test Sulphur Test Protein containing Yellow colour change benzene ring. No appearance of white coagulant.Casein Physical Properties:     Colour : Colourless. No appearance of Protein not black precipitate. Conclusion : The unknown solution is Casein. become brick red with boiling. It is Protein.

Appearance of violet It is Protein. benzene ring. Odour : Odourless.(Neutral) Test Litmus paper Biuret Test Heat Coagulation Test Colour Reaction Test: (d) Xanthoprotein Test . No appearance of Protein not Sulphur Test black precipitate. white coagulant.Gelatine Physical Properties:     Colour : Colourless. No appearance of white coagulant. containing sulphur containing amino acid. (e) Millon Test No appearance of purple ring between two solution. No appearance of It is not Albumin. Protein not containing phenol ring. (f) Rosenheim Test Protein not containing tryptophane. Observation No change. Result The solution is not Casein. Reaction : Neutral. Conclusion : The unknown solution is Gelatine. colour. Protein not No appearance of containing yellow colour. Aspect : Clear.

Result The solution is not Casein. Reaction : Neutral.(Neutral) Test Litmus paper Biuret Test Heat Coagulation Test Colour Reaction Test: (g) Xanthoprotein Test (h) Millon Test (i) Rosenheim Test Sulphur Test Conclusion : The unknown solution is Albumin. . Odour : Fishy. Appearance of violet It is Protein. colour. Protein containing White precipitate phenol ring. Aspect : Turbid. Observation No change. to orange by adding ammonium hydroxide. Appearance of black Protein containing precipitate. Appearance of white It is Albumin. sulphuric containing amino acid. Protein containing Yellow colour change benzene ring. become brick red with boiling. coagulant. ring between two solution. Protein containing Appearance of purple tryptophane.Albumin Physical Properties:     Colour : Whitish.

) Reducing Sugar Barfoid test 1 ml solution + 3 ml Barfoid & boil -ve (No red percipitate) Sucrose +ve ( Immediate red ppt) Monosaccharide Seliwanoff test 1 ml solution + 3ml Seliwanoff & boil -ve (delayed red ppt) Lactose +ve (Red color) Fructose -ve (No red Color) Glucose .General Scheme (CHO) Litmus Paper Neutral (no change in color) Biuret test Alkaline (blue color) Casein -3 ml solution + 3ml NaOH 10% + 1-2 drops CuSO₄ 1% +ve (Violet color) Protein -ve (No violet color) Carbohydrate Iodine test 5 ml of solution + 2 drops diluted iodine +ve (blue color) Starch -ve (No blue color) Not Starch Fehling Test 2 ml solution + 2 ml Fehling & Boil +ve (Red ppt.

Heat upper half +ve (White ppt) -ve (No white ppt) Gelatin Albumin +ve Albumin Colour Reaction Test -ve Gelatin .General Scheme (Protein) Biuret test -3 ml solution + 3ml NaOH 10% + 1-2 drops CuSO₄ 1% -ve (No violet colour) CHO +ve ( violet colour) Protein Litmus Paper Blue colour (Alkaline) Casein Colour Reaction Test ( All +ve) Heat Coagulation (No white ppt) No change (Neutral) Heat Coagulation Fill 2/3 tube with Solution.