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Unit 1 – Biology and Disease
By Jonathan Curtis
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As level Biology notes........................................................................................................1 Contents...............................................................................................................................2 Section 1.1: Causes of Disease - Pathogens......................................................................4 Section 1.2: Epidemiology..................................................................................................6 Section 1.3: Lifestyle and Health........................................................................................7 Section 2.1: Enzymes and Digestion................................................................................10 Section 2.2: Carbohydrates – Monosaccharides..............................................................11 Section 2.3: Carbohydrates – disaccharides and polysaccharides. .................................13 Section 2.4 – Carbohydrate digestion...............................................................................14 Section 2.5 – Proteins.......................................................................................................15 Section 2.6 – Enzyme action............................................................................................17 Section 2.7 – Factors affecting enzyme action.................................................................18 Section 2.8 – Enzyme inhabitation...................................................................................19 Section 3.1 – Investigating the structure of cells.............................................................20 Section 3.2 – The electron microscope.............................................................................22 Section 3.3 – Structure of epithelial cells.........................................................................23 Section 3.4 - Lipids...........................................................................................................25 Section 3.5 – The cell surface membrane.........................................................................26 Section 3.6 - Diffusion.....................................................................................................28 Section 3.7 – Osmosis.......................................................................................................29 Section 3.8 – Active transport .........................................................................................30 Section 3.9 – Absorption in the small intestine................................................................31 Section 3.10 – Cholera .....................................................................................................32 Section 3.11- Oral rehydration therapy ...........................................................................33 Section 4.1 – Structure of the human gas-exchange system............................................34 Section 4.2 – The mechanism of breathing......................................................................36 Section 4.3 – Exchange of gas in the lungs .....................................................................37 Section 4.4 – Pulmonary tuberculosis..............................................................................39 Section 4.5 – Fibrosis, asthma and Emphysema .............................................................42 Section 5.1 – The heart and heart disease.........................................................................44 Section 5.2 – The cardiac Cycle ......................................................................................47 Section 5.3 – Heart Disease..............................................................................................51 Section 6.1 – Defence mechanisms .................................................................................53 Section 6.2 – Phagocytosis...............................................................................................54 Section 6.3 – T cells and cell-mediated immunity...........................................................55 Section 6.4 – B cells and humoral immunity ..................................................................56 Section 6.5 – Antibodies...................................................................................................57 Section 6.6 – Vaccination ................................................................................................58 Section 7.1 – Investigating Variation...............................................................................60 Section 7.2 – Types of variation ......................................................................................62 Section 8.1 – Structure of DNA........................................................................................63 Section 8.2 – The triplet code ..........................................................................................66 Section 8.3 – DNA and Chromosomes.............................................................................68 Section 8.4 – Meiosis and Genetic Variation ..................................................................71 Section 9.1 – Genetic Diversity........................................................................................73 Section 10.1 – Haemoglobin.............................................................................................74 Section 10.2 – Oxygen dissociation curves .....................................................................75 Section 10.3 – Starch, glycogen and cellulose ................................................................76 Section 10.4 - Plant cell structure.....................................................................................77 Page 2 of 114
Section 11.1 – Replication of DNA..................................................................................78 Section 11.2 – Mitosis......................................................................................................80 Section 11.3 – The cell cycle............................................................................................83 Section 12.1 – Cellular organisms....................................................................................85 Section 13.1 – Exchange between organisms and their Environment ............................87 Section 13.2 – Gas exchange in single celled organisms and insects .............................88 Section 13.3 – Gas exchange in Fish................................................................................89 Section 13.4 – Gas exchange in the leaf of a plant .........................................................90 Section 13.5 – Circulatory system of a mammal ............................................................91 Section 13.6 – Blood vessels and their functions ............................................................93 Section 13.7 – Movement of water through roots ...........................................................95 Section 13.8 – Movement of water up stems ..................................................................97 Section 13.9 – Transpiration and factors affecting it.......................................................98 Section 13.10 – Limiting water loss in plants .................................................................99 Section 14.1 – Classification .........................................................................................100 Section 15.1 - Genetic comparisons using DNA and proteins ......................................104 Section 15.2- Courtship behaviour ................................................................................105 Section 16.1 – Genetic variation in bacteria ..................................................................106 Section 16.2+16.3 – Antibiotics/Antibiotic use and resistance.....................................109 Section 17.1 – Species diversity ....................................................................................112 Section 17.2 – Species diversity and Human activities .................................................113
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Huntington’s disease etc. Skin – Difficult to penetrate. U. Infection – process by which a pathogen enters and establishes it’s self. schizophrenia. free from disease.V rays. down syndrome. Organs and tissue may not work as well due to slower cell renewal and repair. Degenerate – wholly/partly caused by aging.g. Gas exchange – airborne pathogens Page 4 of 114 . lung cancer. thick and water proof.Pathogens • • • • • • • • • • Health – A state of physical and mental well-being. STIs. Platelets quickly produce scabs. • • • • Communicable disease – spread via close proximity or contact. Nutritional deficiency – caused by inadequate or unbalanced diet or by overeating E. obesity.g. Environmental – Abnormal bodily reaction caused by the environment e. Interface – where internal and an external environment meet. Disease – An abnormal condition of an organism that impairs bodily functions and is associated with specific symptoms. Non-Communicable – disease caused by food/drink or animal vectors e.g. Interfaces are adapted for absorption but also make it easier for pathogens to pass through. Psychological disorders – diseases causing changes in the working of the brain E. cystic fibrosis. Social/self induced – Influenced by living conditions or personal behaviour e. Infectious – Caused by pathogens Inherited – due to a mistake or alternation in the genetic make-up e.g. Non-infectious – Sometimes called disorders and can be caused by a broad range of environmental factors. mosquitoes.1: Causes of Disease . scurvy. They cannot be transferred.g. etc. etc. rickets.Section 1. Most microbes are harmless Pathogen –disease causing microbe. Note: many diseases can be caused by multiple factors.g.
Protease also kills microbes. It should be possible to isolate the microbes from the new host. they are introduced to a healthy host to see if it is infected. 2. Bacteria reproduction is called binary fusion. Celia that is on the epithelial cells work together to remove microbes. Endemic – a disease that is always present in the population. It must be possible to isolate and grow the microbe. 4. Defences Gas exchange – thick/sticky mucus traps pathogens. Pandemic – when an outbreak occurs on a global basic. 3. It kills bodily cells and rarely produces toxins. Digestive system – concentrated HCl kills microbes.Digestive System – Disease in contaminated food or water. Fungal toxins are called mycotoxins. When cultured. Bacteria present when disease is present. Robert Koch 1. Viruses – Invade bodily cells in order to reproduce thus preventing the host cell functioning as normal. Page 5 of 114 . Fungi – Opportunistic pathogens. Epidemic – when a new disease spreads rapidly through the population.
positive correlation A positive correlation will occur when an increase in one variable. Negative Correlation A negative correlation will occur when an increase in one variable causes a decrease in another. Weak correlation A weak correlation occurs when there is a wide spread of data shown in the graph. Strong. Data must be analysed over long periods of time. A correlation is different causal link. Variables must be controlled. In order for the correlation to be “strong”. from high birth rate.2: Epidemiology Is the study of patterns in diseases and the various factors that effect the spread of disease. there must be little spread in the data.g. Page 6 of 114 .Section 1. How to prove a link • • • Wide samples must be used. Demographic Transition Explains how the population changes over time e. causes a increase in another.
Cancer – Cell division in an uncontrollable fashion. Lifestyle factors can expose you to more carcinogens. Age + = more likely. there is little cause for concern. We need to look at probability that a hazard will occur as a consequence of the hazardous event.3: Lifestyle and Health • • • • Risk – A measure of probability that damage to health will occur as a result of a given hazard. Risk must be relative. Measuring risk • • • • • • • • • • • 0% (no harm will occur) 100% (will defiantly occur) A timescale is needed to give the data more weight. This continues if there are nutrients. Cancer cells cease to function normally. however can cause problems depending on where it grows. Usually harmless.Section 1. Maligment – grow faster and can spread throughout the body. Benign – does not move from the point of origin. One mutated cell can cause a mass of mutated cells. If the consequence of the hazard is high and the probability is low. They are cancer causing agents. Most mutated cells are destroyed. Can have its own blood supply. Not fully understood how cancer starts. A major concern is when both are high. The process of moving to another area of the body is called metastasis. Page 7 of 114 • • • • . Carcinogen – Cause the DNA to mutate. Tumour producing genes (oncogenes). Genetics can cause approximately 5% of cancers.
Radiotherapy – ionising radiation that destroys tissue. Early diagnosis. The gene that mutates is called a tumour suppressor gene. Smoking • • Heavy smoking over a long period of time will drastically increase the risk of developing lung cancer. Effects all cells that divide rapidly. Alcohol – increases risk. Page 8 of 114 . high fibre. uv light and xrays are carcinogens. Physical activity – exercise reduces the risk. higher the risk.• • • • • • More you smoke. There is a strong correlation. Healthy cells suffer less so there are little side-effects. Surgical removal – Easiest when the tumour is benign. Conclusive evidence • • • Tar in cigarettes contains Benzopyrene (carcinogen) Cancer cells were looked at and scientist found that mutations occurred in 3 places in the DNA. Treatments • • • • • Prevention is better than cure. fruit etc. Future treatments • • Hyperthermia may destroy cancer because the immune system is better at detecting cancer cells. Radiation. It may be possible to create drugs which can locate genes which are responsible for mutating and causing each type of cancer. Hormones – high level of sex hormones can increase risk. but not a causal link. Chemotherapy – using drugs to kill cells in the body. Diet – low fat.
Page 9 of 114 . It is only a correlation because it is a multi-factorial disease. Anaerobic respiration does not release enough energy. If this happens to coronary arteries it is called coronary thrombosis. Exercise can lower blood pressure. Coronary Heart Disease • • • • • • • • • • • • Largest cause of death in the U.K Occurs when one of the arteries supplying heart tissue with oxygen is blocked. Blood clot – thrombus Process of a blood clot forming is called thrombosis. Heart attack – myocardial infarction. thus increase blood pressure. Diets high in saturated fats will increase the risk of developing coronary heart disease. High blood pressure increase the rate at which cholesterol is deposited. Heart respires anerobically when there is a blood clot. even though it is very likely to be the cause.• • This is still not a causal link because smoking does not defiantly cause cancer. Smoking narrows blood vessels.
giving them a larger surface area. Mucus is also produced in the stomach by glands. The large intestine absorbs water. the food becomes drier. The surface area of villi is further increased by millions of tinier projections called microvilli. There are glands within it that produce enzymes to digest protein. The rectum is where faeces is stored before it is removed through the anus in a process called egestion. The microvilli are found on the epithelial cells of each villus. The stomach is a muscular sac with an inner layer that produces enzymes. The small intestine is a long muscular tube. as appose to digestion. Often the water is reabsorbed by the secretion of digestive glands.1: Enzymes and Digestion • • Glands produce enzymes that are used to break down large molecules into smaller ones that are ready for abortion.Section 2. Because there is little water within the large intestine. • • • • Page 10 of 114 . Therefore it used for transport. The mucus prevents the stomach being digested by its own enzymes. The enzymes enter the small intestine through its walls and through glands. The digestive system provides an interface between the body and the environment because it allows food to pass through it. Its roles are to store and digest food (especially proteins). thus forming faeces. Major parts of the digestive system • The Oesophagus is made up of a thick muscular wall and is adapted so that food can pass down it easily from the mouth to the stomach. The inner walls of the small intestine are folded into villi. Food is further digested by enzymes in the small intestine. This adapts the small intestine so that it can absorb substances into the blood stream.
These smaller sections are then hydrolysed into even smaller molecules by additional enzymes. Once these molecules have been broken down to become even smaller molecules such as monosaccharides. This makes it possible to not only absorb food but to increase its surface area. This is called assimilation. This is carried out by enzymes. an enzyme will break down a molecule into smaller sections. Lipase breaks down lips into glycerol and fatty acids. The general term for these enzymes is hydrolases. Because enzymes are specific. There are two stages of digestion. Hydrolysis is the process of splitting up molecules by adding water to the bonds that hold them together. lipase and amylase.2: Carbohydrates – Monosaccharides Page 11 of 114 . This secretion will contain the enzyme amylase. This contains protease. These new molecules are incorporated into body tissue or are used in processes within the body. Carbohydrases break starch molecules down until they become monosaccharides. more than one is needed to break down a large molecule. Section 2. Usually. physical breakdown and chemical absorption. Physical breakdown Large pieces of food are broken down into smaller pieces by processes such as chewing and the churning of food in the stomach. Chemical digestion Chemical digestion is the process of breaking down large molecules into smaller ones so that they can be absorbed. Protease breaks protein down to amino acids. thus making it easier for chemical absorption. It secretes pancreatic juice. They pass there secretion via a duct into the mouth. The pancreas is a large gland situated near the stomach. they are absorbed into the body and are often built up again to form larger molecules again. Enzymes function by hydrolysis.• • The salivary glands are positioned near the mouth.
Although its molecular arrangement is often shown as a straight line. Glucose is a hexose because it has 6 carbon atoms and has the formula C6H12O6 Even though it has the same chemical formula. Glucose Galactose Fructose Glucose is the most common sugar.• Carbohydrates are carbon molecules (carbo) combined with water molecules (hydrate). Life based on Carbon • • Carbon atoms are able to readily form bonds with other carbon atoms Life on earth is based on the versatile carbon atom. However on the left of the diagram you can see how the Hydroxide and hydrogen atoms are arranged differently to glucose. Page 12 of 114 . N can be any number from 3 -7. its atoms form a ring. The making of Large molecules • • Carbohydrates are long chains made up of individual molecules called monosaccharides. A pair of monosaccharides is called a disaccharide and several monosaccharides joined together is called a polysaccharide. Fructose has a very different structure to glucose and is often used as a sweetener. Monosaccharides • • • Monosaccharides are soluble and have the general formula (CH20)n. the hydrogen and oxygen atoms can be arranged in many different ways. Galactose has the same chemical formula as glucose.
This is called a condensation reaction. but instead are used to give support to plant cells. polysaccharides break down into disaccharides or monosaccharides. This means that they are very suitable for storage. If starch is present. Disaccharides When combined in pairs monosaccharides form disaccharides. • • • Polysaccharides • • • • Polysaccharides are long chains of monosaccharides combined together through glycosidic bonds. • • • Glucose with glucose forms maltose Glucose with fructose forms sucrose Glucose linked with Galactose forms lactose. Test for starch To test for starch. In order to break the bond. Because they are very long molecules. add potassium iodine solution. Test for non-reducing sugars To test for a non reducing sugar it must first be hydrolysed then added to Benedict’s reagent. the iodine will turn from yellow to blue-black. Some polysaccharides such as starch are not used for storage. they are often insoluble. When hydrolysed. A glycosidic bond is an oxygen atom. • When two monosaccharides join together a water molecule is removed. The bond holding the two monomers together is called a glycosidic bond. water is added to the molecule in a process called hydrolysis.Section 2. Page 13 of 114 .3: Carbohydrates – disaccharides and polysaccharides.
Normally one enzyme breaks a large molecule into smaller sections. The pancreatic juice contains pancreatic amylase which hydrolyses the remaining starch. Page 14 of 114 . Sucrase which is produced by the epithelial lining breaks down sucrose into fructose and glucose. The epithelial lining of the intestine produces the enzyme maltase. Firstly the enzyme “amylase” is produced in the mouth by salivary glands. food passes into the small intestine where it mixes with pancreatic juices. where the pH is kept at neutral by mineral salts. the enzyme is destroyed by the stomach acid where the pH is around 2. This means that no more starch can be digested. Alkaline salts are produced by the intestine wall and the pancreas to maintain the pH at neutral so that the enzymes can work efficiently. After the stomach. To overcome the problem of lactose intolerance amongst children. nausea and diarrhoea. bacteria digest it and produce lots of gas. For new born babies.4 – Carbohydrate digestion • • • • • • • • • • • • • • • It usually takes more than one enzyme to break down a large molecule. This breaks maltose into glucose. When undigested lactose enters the small intestine. and then other enzymes break these down to monomers. This enzyme breaks starch into maltose by hydrolysing the glycosidic bonds holding the molecule together. Food is physically broken down by teeth to increase surface area. milk makes up the majority of their diet.Section 2. Once the food is swallowed. People who are lactose intolerant do not produce enough lactase to break down the lactose found in milk. lactose can be pre-digested before consumption. This can cause stomach cramps.
The hydrogen atom (H) The r group. They create a water molecule by combining the OH from the carboxyl group of one amino acid with the hydrogen atom of another amino acid. The formation of a peptide bond Through the same process by which monosaccharide join to make disaccharides and polysaccharides. The carboxyl group (COOH) this is an acid group. Each amino acid has a different r group. this can be a variety of chemicals. There is: The amino group (NH2) this is a basic part of the molecule where it gets the name amino. Primary Structure After many condensation reactions (removal of water molecules to form a peptide bond).5 – Proteins • • • Each organism has numerous proteins that differ from species to species.Section 2. Page 15 of 114 . Proteins are the most important molecules for life. When there is a repeating sequence of amino acids joined by a peptide bond it is called a polypeptide chain. This repeating sequence of amino acids in a polypeptide chain is known as the primary structure. Structure of amino acids There are 4 main parts that make up the general structure of an amino acid. The structure of one protein molecule differs from that of all other protein molecules. The chain of many amino acids is called a poly peptide. amino acids can join together to form dipeptides. many monomers are joined together in a process called polymerisation.
which is an alpha helix can be further twisted and folded forming a unique 3D structure for each protein. They are weaker than disulphide bonds. not easily broken down. Page 16 of 114 . Hydrogen bonds – there are many of these however they are easily broken down. It is formed by several different types of bonds. A change in pH can affect an ionic bond. Disulphide bond – fairly strong. Tertiary Structure The secondary structure. This causes the long chain to twist in on its self creating a coil known as a alpha helix. Quaternary Structure This structure appears when a number of complex molecules containing polypeptide chains that are linked in various ways are associated with non-protein molecules called prosthetic groups.Secondary structure The secondary structure is formed when the -C=O (which has a overall negative charge) is attracted to the hydrogen atom (which has an overall positive charge). Ionic bonds – formed by the carboxyl and amino groups.
Enzymes work by lowering the activation energy required to start a reaction Once the substrate is inside the active site. and so speeding up the reaction. • It's a bit more complicated than that though. This is sometimes called the induced fit mechanism. Although enzymes can change the speed of a chemical reaction. distorting the molecule in the active site. the active site change shape and fits itself around the molecule. Page 17 of 114 . distorting it into forming the transition state. rate at an optimum state The substrate and the enzyme must collide with sufficient energy. otherwise they could make "impossible" reactions happen and break the laws of thermodynamics. the enzyme changes shape slightly.6 – Enzyme action • • • • • • • • • All enzymes are globular proteins → spherical in shape Control biochemical reactions in cells They have the suffix "-ase" Intracellular enzymes are found inside the cell Extracellular enzymes act outside the cell Enzymes are catalysts → speed up chemical reactions Reduce activation energy required to start a reaction between molecules Substrates (reactants) are converted into products Reaction may not take place in absence of enzymes (each enzyme has a specific catalytic action) • • • • Enzymes catalyse a reaction at max. they cannot change its direction. and making it more likely to change into the product. • When a substrate (or product) molecule binds.Section 2.
e. For mammalian enzymes this is about 40°C. and enzymes from thermophilic bacteria work at 90°C. so the enzyme (and especially the active site) loses its shape to become a random coil. such as protease enzymes in animal stomachs. once it reaches its optimum temperature it will begin to decrease as the temperature rises due to the active site being denatured. which have an optimum of pH 1. This is how long it takes to run its course. For example a carboxyl acid R groups will be uncharged a low pH (COOH).7 – Factors affecting enzyme action Measuring enzyme-catalysed reactions • • To measure the progress of an enzyme-catalysed reaction. but there are enzymes that work best at very different temperatures. • Page 18 of 114 .Section 2. Effect of Temperature • Enzymes have an optimum temperature at which they work fastest. but a few enzymes can work at extreme pH. The thermal energy breaks the hydrogen bonds holding the secondary and tertiary structure of the enzyme together. For most enzymes this is about pH 7-8 (physiological pH of most cells). so the properties of the active site change and the substrate can no longer bind. The rate of reaction will increase as temperature increases. The two “events” most frequently measured are the volume of gas produced during a reaction and the disappearance of a substrate. approximately almost every ten degrees. enzymes from the arctic snow flea work at -10°C. The pH affects the charge of the amino acids at the active site. The rate of reaction doubles. Then.g. its time course is measured. but charged at high pH (COO-). • • • Effect of pH • • Enzymes have an optimum pH at which they work fastest.
so the reaction is slower. It therefore competes with the substrate for the active site. They are found naturally. but are also used artificially as drugs. Competitive inhibitor • • A competitive inhibitor molecule has a similar structure to the normal substrate molecule. including the active site. changing the shape of the whole enzyme. heavy metal ions and some insecticides are all non-competitive inhibitors. It is the difference between the concentration of the inhibitor and the concentration of the substrate that determines the affect it has on the enzymes activity. Eventually all the substrate molecules will be in the active sites. while those that tightly and cannot be washed out are called irreversible inhibitors. depending on the concentration of the inhibitor. There are two kinds of inhibitors. Inhibitors that bind fairly weakly and can washed out are sometimes called reversible inhibitors. Non-competitive inhibitors therefore simply reduce the amount of active enzyme (just like decreasing the enzyme concentration). However. so that it can no longer bind substrate molecules. Poisons like cyanide. the longer this will take. be bind • • Page 19 of 114 . pesticides and research tools. and it can fit into the active site of the enzyme.8 – Enzyme inhabitation Inhibitors inhibit the activity of enzymes.Section 2. reducing the rate of their reactions. The inhibitor is not permanently bonded to the active site so once it leaves a substrate molecule can take its place. • • • Non-competitive inhibitors • Non-competitive inhibitors do not fit into the active site but instead they bind to another part of the enzyme molecule.
An isotonic solution is one that has the same water potential as the original tissue. Magnification When viewed under a microscope. It can be found using the following formula: Magnification=size of image/size of object The previous formula can also be rearranged to find the size of an object. The greater the resolution.Section 3. Cell fractionation Cell fractionation is the process where cells are broken up and the different organelles they contain are separated out. It is then filtered to remove complete cells and large pieces of debris. Magnification tells you how many times bigger the image is in relation to the actual size of the object. Beams of electrons have shorter wavelengths and are therefore able to distinguish between objects as close as 0.1 – Investigating the structure of cells Microscopy Lenses work more effectively if they are in a compound light microscope. Before fractionation begins. Page 20 of 114 . the greater the clarity of the image that is produced.1nm apart. Isotonic – to prevent organelles bursting or shrinking as a result of osmotic gain or loss of water. they can only distinguish between objects that are at least 0. The fluid is called a homogenate. Light waves a have a relatively long wavelength. the material seen in called an image. Buffered – to maintain a constant pH Homogenation Cells are broken up by a homogeniser that releases the organelles.2micrometers apart. Size of object=size of image/magnification Resolution The resolving power of a microscope is the minimum distance two objects can be apart in order for them to appear as separate items. therefore. the cells are but in a solution that is: Cold – to reduce enzyme activity that might break down the organelles.
creating a centrifugal force that forces the mixture to separate. called the supernatant is removed.Ultracentrifugation Ultracentrifugation is the process by which the homogenate is separated in a machine called a centrifuge. The supernatant is then put in another tube where it is spun at an even higher speed than before. The spins tubes of the homogenate. The heaviest organelles such as the nucleus are forced to the bottom where they form a thin sediment. The fluid at the top. leaving just the sediment of nuclei at the bottom. • • • • • The tube of filtrate is placed in the ultracentrifuge and spun at a slow speed. The next heaviest organelles (mitochondria) are forced the bottom and the process continues until all the organelles are separated. Page 21 of 114 .
Section 3. Because electrons are absorbed by molecules in the air.2 – The electron microscope Electrons have a shorter wavelength than light and so they have a greater resolving power. Artefacts may appear on the image. Some of the electrons are absorbed by the specimen and appear dark on the image. Because the process takes place in a vacuum. these appear as a result of the way the specimen is prepared. Transmission electron microscope The TEM consists of a gun that fires electrons which are focused onto the specimen be a condenser electromagnet. living specimens cannot be observed. This produces an image of the specimen. The beam of electrons is directed over the surface of the specimen in a regular pattern. The image that appears on screen is called a photomicrograph. The specimen must be extremely thin. As electrons are negatively charged. The SEM has a lower resolving power than the TEM (20nm) but is still ten times better than a light microscope. the beam can be focused using an electromagnet. A complex staining process is required and even then the image is only in B&W. other parts allow the electrons through and so appear light. There are two types of electron microscope: . The electrons bounce on the contours of the specimen and are scattered.Transmission electron microscope and scanning electron microscope. a near vacuum must be created within the chamber of an electron microscope for it to work effectively. Scanning electron microscope All the limitations of the TEM apply to the SEM but the specimen does not have to extremely thin as the electrons do not penetrate. The scattering of the electrons can be analysed and from this an image can be produced using a computer. Page 22 of 114 .
Section 3.3 – Structure of epithelial cells
Epithelial cells are eukaryotic cells. Eukaryotic cells have a distinct nucleus and a membrane that surrounds each organelle. The function of an epithelial cells is top absorb and secrete The nucleus The nucleus controls the cells activities and contains hereditary material. • The Nuclear envelope is a double membrane that surrounds the nucleus. Its outer membrane is continuous with the endoplasmic reticulum and often has ribosomes on its surface. It can control the substances entering and leaving the nucleus.
• • • •
Nuclear pores allow the passage of large materials into and out of the nucleus. Nucleoplasm is granular jelly like material that makes up the bulk of the nucleus. Chromatin is the DNA found within the nucleoplasm This is the diffuse form chromosomes take up when the cells is not dividing. The nucleolus is small spherical body within the nucleoplasm. It manufactures ribosomal RNA and assembles ribosomes.
The mitochondria • A double membrane surrounds the organelle, the outer one controlling the entry and exit of material. The inner membrane inner membrane is folded to form extensions known as cristae. Cristae are shelf like extensions of the inner membrane. These provide a large surface area for the attachment of enzymes during respiration.
• The matrix makes up the remainder of the mitochondria. It is a semi-rigid material that contains proteins, lipids and traces Page 23 of 114
of DNA that allows the mitochondria to control the production of its own proteins. The enzymes involved in respiration are found in the matrix. Mitochondria are responsible for the production of the energy-carrier molecule ATP. Because of this, high numbers of mitochondria are found in cells where there is a high level of metabolic activity. Endoplasmic Reticulum Rough endoplasmic reticulum – has ribosomes present on the outer surface of the membranes. Its functions are to: a) provide a large surface area for the synthesis of proteins and glycoproteins, b) provide a pathway for the transport of materials, especially proteins throughout the cell. Smooth endoplasmic reticulum lacks ribosomes on its surface and is often more tubular in appearance. Its functions are to: a) synthesise, store and transport lipids, b) synthesise store and transport carbohydrates. Golgi Apparatus The Golgi apparatus is similar to the SER in structure but is more compact. It consists of a stack of membranes that form flattened sacks, or cisternae with small rounded hollow structures called vesicles. The proteins and lipids produced in the ER are passed through the Golgi apparatus in strict sequence. The Golgi apparatus modifies these proteins often by adding non-protein structures to them such as carbohydrates. It is also labels them so they can be sorted and sent to their correct destination. Once sorted and modified, proteins are transported in vesicles which are regularly removed from the edge of the Golgi cisternae. These vesicles move to the cell membrane where they fuse and release their contents to the outside. Lysosomes
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Lysosomes are formed when a vesicle contains enzymes. Lysosomes isolate potentially dangerous enzymes from the rest of the cell before releasing them outside of the cell or into phagocytic vesicles within the cell. Lysosomes digest worn out organelles so that the useful chemicals they are made of can be reused. They can completely break down cells after they have died. (Autolysis) Ribosomes Ribosomes occur in either the cytoplasm or the RER. There are two types depending on which cell they are found in: 80S Type – found in eukaryotic cells, is around 25nm in diameter. 70S Type – found in prokaryotic cells, is slightly smaller. Microvilli Microvilli are finger like projections of the epithelial cells. There function is to increase the surface area for diffusion.
Section 3.4 - Lipids
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Take a dry. Lipids are insoluble in water and are therefore suitable for waterproofing. Roles of lipids Phospholipids contribute to the flexibility of membranes and the transfer of lipidsoluble substances across them. The proportion of oxygen to carbon and hydrogen is smaller than in carbohydrates. grease free test tube. hydrogen and oxygen.• • • • Lips contain carbon. Fatty acid molecules repel water whereas phosphate molecules are attracted to water. 2.5 – The cell surface membrane Page 26 of 114 . A cloudy white colour indicates the presence of a lipid. Waterproofing. lipids can be used as: An energy source. Triglycerides are so called because they have three fatty acids (tri) combined with glycerol (glyceride). Often stored around delicate organs. 5. 4. Fats are slow conductors of heat. CH2OH + HOOC CH2OOC + H2O (Glycerol) + (fatty acid) Phospholipids Phospholipids are similar to lipids except that the fatty acid is replaced with a phosphate molecule. They are insoluble in water. Each fatty acids combines with glycerol in a condensation reaction. Lipids can provide more than twice the energy of carbohydrate. Shake the test tube and dissolve the lipids. Insulation. Take 2cm^3 of the sample being tested and add 5cm^3 of ethanol. They are soluble in organic solvents such as alcohol and acetone. kept under skin to retain heat in the body. Test for lipids 1. In addition to this. Protection. Add 5cm^3 of water and shake gently. 3. Section 3.
The hydrophobic tales point inwards. Phospholipids Phospholipids are important in cell surface membranes because: • • One layer of phospholipids has its hydrophilic head pointing inwards towards the water in the cytoplasm. Make the membrane more flexible. Page 27 of 114 . interacting with the water surrounding the cell. Intrinsic proteins – Span the phospholipids bilayer.The cell-surface membrane is a plasma membrane that surrounds that surrounds cells and forms a boundary between the cytoplasm and the environment. Prevent water-soluble substances entering and leaving the cell. Mosaic – because the proteins are imbedded in the structure in a similar way that stones are imbedded in a mosaic. Some transport water soluble molecules across the membrane others are enzymes. Proteins The proteins in the phospholipids bilayer are arranged randomly in two main ways: Extrinsic proteins – appear on the surface or partially imbedded. giving it a flexible structure.because the phospholipids molecules can move relative to each other. etc. The other has its head pointing outwards. The function of phospholipids in the cell-membrane are to: allow lipid-soluble substances to enter and leave the cell. Fluid-mosaic model of the cell surface membrane Fluid . act as cell receptors for molecules such as hormones. They provide mechanical support or when in conjunction with glycolipids. Protein molecules in the membrane allow active transport by forming ion channels for sodium. potassium.
When a molecule that is specific to the carrier protein is present. Page 28 of 114 .Section 3. causing it to release the molecule on the other side of the plasma membrane. the faster the rate of diffusion. Facilitated diffusion can only occur at specific point along the plasma membrane where there are special protein molecules. the greater the rate of diffusion The larger the area of an exchange surface. The proteins for special water filled channels. Such molecules such as glucose and amino acids would take much longer to diffuse through the phospholipids bilayer. The size and nature of the diffusing molecule. The motion is random and there is no set pattern to the way they move. This allows water soluble ions and molecules to pass through. Rate of diffusion • • • • • The greater the difference in concentration. its composition and the number of pores. All particles are constantly in motion due to the kinetic energy that they posses. The channels only open for specific molecules. the greater the rate of diffusion.Diffusion Diffusion is defined as the net movement of molecules or ions from a region where they are more highly concentrated to one where their concentration is lower. Diffusion is proportional to: surface area x difference in concentration Length of diffusion path Facilitated diffusion Facilitated is a passive process as it only relies on the kinetic motion of particles. For example smaller molecules diffuse faster than big ones.6 . the carrier protein changes shape. The nature of the plasma membrane. The thinner the exchange surface.
cells are often bathed in solutions where the water potential outside the cell is the same as the water potential inside the cell. Page 29 of 114 .7 – Osmosis Osmosis is defined as the passage of water from a region where it has a higher water potential to a region where it has a lower water potential through a partially permeable membrane.Section 3. Osmosis in animal cells If a red blood cell is place in pure water it will absorb water by osmosis because it has a lower water potential. A hypertonic solution is one where the water potential outside the cell is lower than the water potential inside the cell. This is called an isotonic solution. Water potential is measured in Pascal’s. At the point where the water potentials on either side of a partially permeable membrane are equal. Water with a solute dissolved in it will have a water potential that is less than 0. Water molecules move from one side where the water potential is higher (less negative) across a partially permeable membrane to another side where the water potential is lower (more negative). A hypotonic solution is one where the concentration outside is greater than the concentration inside. Under standard conditions of temperature (25c). The water moves along a water potential gradient. To prevent cells bursting due to too much water entering the cells. a dynamic equilibrium is established and there is no net movement of water. pure water is said to have a water potential of 0. The cell-surface membrane will eventually burst if too much water enters the cells.
On the other side of the membrane ATP bind to the carrier protein causing it to split into ADP and a phosphate molecule. The phosphate molecule then recombines with the ADP to form ATP again. the molecule or ion is moved into the cell at the same time as a different one is being removed from it. Metabolic energy is required for this process. Active transport is the movement of molecules or ions into or out of a cell from a region of lower concentration to a region on higher concentration using energy and carrier molecules. This is known as co-transport. The molecules of the ions bind to the receptors on the channels of the carrier protein. causes the carrier protein to change shape. Page 30 of 114 . This as a result. Carrier molecules which act as “pumps” are involved.8 – Active transport Active transport allows cells to exchange molecules against a concentration gradient. which causes the carrier protein to revert back to its original shape. releasing the molecule onto the other side. • Occasionally. Metabolic energy is needed in the form ATP.Section 3. • Active transport uses ATP in two main ways: by using ATP to directly move molecules. The carrier molecules accept molecules or ions to be transport on one side of it. • • • • By using a concentration gradient that has already been set up by direct active transport. One example of this is the sodiumpotassium pump Sodium ions are actively taken in by the cell whilst potassium ones are actively removed from the cell.
3. Their properties increase the efficiency of absorption because: • • • • They increase the surface area for diffusion They are very thin walled. Sodium ions are actively transported out of the epithelial cells by the sodium potassium pump. They are situated on the cell surface membrane. 4.9 – Absorption in the small intestine Villi and Microvilli Villi have walls lined with epithelial cells. new glucose rich food replaces it. Page 31 of 114 . They are able to move and so maintain a concentration gradient They are well supplied with blood vessels so that the blood can carry away absorbed molecules and hence maintain a diffusion gradient. As they flood back into the cells. The glucose diffuses into the blood through a carrier protein. they are coupled with glucose molecules which are drawn in with them. There is now a much higher concentration of sodium ions in the lumen than in the cells.Section 3. that powers the movement of glucose into the cell. 2. Role of active transport in absorption The way in which most glucose is absorbed from small intestine is an example of cotransport. 1. thus reducing the distance over which diffusion takes place. Villi are situated at the interface between the lumen of the intestines and the blood and other tissues inside the body. The epithelial cells possess Microvilli which further increase the surface area for diffusion. thus maintains a concentration gradient for diffusion. rather than the ATP directly. The sodium ions diffuse into the cells down a concentration gradient. It is the sodium ion concentration. Villi contain muscles which move the food ensuring the glucose is absorbed from the food adjacent to the villi.
(70s type) Bacteria store food as glycogen granules and oil droplets. The loss of chloride ions from cells increases the water potential in the cell. Page 32 of 114 .5. • • • How the cholera bacterium causes disease Almost all Vibrio cholerae bacteria ingested by humans are killed by the low pH in the stomach but many can still survive. Many bacteria also protect themselves by producing a capsule of mucilaginous slime around this wall. They then start to produce a toxic protein. The genetic material of a bacterium is found in the form of a circular strand of DNA. The causes the ion channels of the cell-surface membrane to open. The protein has two parts: one part binds to the carbohydrate receptors of the intestinal epithelial cells. When the bacteria enter the lumen of the small intestine they use their flagella to propel themselves through the mucus lining of the intestinal wall. Ions move by diffusion into the epithelial cells. but lowers the water potential outside the cells. This causes water to move into the small intestine. Separate from this are smaller circular pieces of DNA.10 – Cholera Structure of a bacteria cell The bacterium that causes cholera is called Vibrio Cholerae.Section 3. whereas the other part enters the epithelial cells. especially if the pH is above 4. This is a mixture of polysaccharides and peptides. Flagella occur at certain types of bacteria. called plasmids. The loss of ions from the cells establishes a concentration gradient. Inside the cell-surface membrane is the cytoplasm that contains ribosomes that are smaller than the ones found in eukaryotic cells. that the chloride ions that are normally contained within the epithelial cells flood into the lumen of the intestine. This establishes a water potential gradient that causes water to move by osmosis from the blood and other tissues into the small intestine. • • • • All bacteria possess a cells wall that is made up of peptidoglycan.
As sodium ions are being absorbed. as in the case of cholera.Oral rehydration therapy What causes diarrhoea? • • • Damage to the epithelial cells in the lining of the small intestine Loss of Microvilli due to toxins Excessive secretion of water due to toxins What is oral rehydration therapy? Drinking water to treat diarrhoea is ineffective because: Water is not being absorbed by the intestine. Indeed. The drinking water does not replace electrolytes that are being lost from cells of the intestine.Section 3.11. Glucose – to stimulate the uptake of sodium ions from the intestine and to provide energy Potassium – to replace lost potassium ions and to stimulate appetite Other electrolytes – such as chloride and citrate ions. to help prevent electrolyte imbalance The solution must be given regularly and in large amounts whilst the person has the illness. Page 33 of 114 . water is actually being lose from cells. the water potential falls and water enters the cells by osmosis. A rehydration solution should therefore contain: • • • • • Water – to rehydrate tissues Sodium – to replace the ions lost from the epithelium of the small intestine and to make optimum use of the sodium-glucose carrier proteins.
The bronchi themselves divide many times before branching into smaller airways called bronchioles.Section 4. and has a smaller volume than the right lung it shares space in the left side of the chest with the heart. which end in tiny sacs called alveoli. on its medial surface for the apex of the heart. narrower. thus changing the size of the airway. The larger airways resemble an upside-down tree. Mammalian Lungs • Lungs are kept inside the body because air is not dense enough to support and protect these delicate structures. Lungs provide efficient surface area for effective gas exchange. These are the narrowest airways – as small as one half of a millimeter across. The lungs are a pair of lobed structures made up of a series of bronchioles. The volume of oxygen that needs to be absorbed and the volume of carbon dioxide that needs to be removed is large in mammals because there is a large volume of respiring cells. Mammals must also maintain a high temperature and therefore have high metabolic and respiratory rates. The trachea (windpipe) branches into two smaller airways: the left and right bronchi. fibrous connective tissue called cartilage. The left lung has two lobes. The left lung is longer. The right lung is divided into three lobes and each lobe is supplied by one of the secondary bronchi. which lead to the two lungs. At the end of each bronchiole are thousands of small air sacs called alveoli. The extremely thin barrier between air and capillaries allows • • • • Page 34 of 114 . called the cardiac notch.1 – Structure of the human gas-exchange system All aerobic organisms require a constant supply of oxygen to release energy in the form of ATP during respiration. In addition to this. Together. the millions of alveoli of the lungs form a surface of more than 100 square meters. The airways are held open by flexible. Within the alveolar walls is a dense network of tiny blood vessels called capillaries. keeping them inside the body prevents loss of water and so they will not dry out easily. which is why this part of the respiratory system is often called the bronchial tree. It has an indentation. Circular airway muscles can dilate or constrict the airways.
or root. The lungs are soft and spongy because they are mostly air spaces surrounded by the alveolar cells and elastic connective tissue. The visceral pleura is firmly attached to the surface of the lung. It contains a thin film of serous fluid that is produced by the pleura. This is where the bronchi. the visceral pleura is continuous with the parietal pleura that lines the wall of the thorax. and nerves enter the lungs. lymphatics.oxygen to move from the alveoli into the blood and allows carbon dioxide to move from the blood in the capillaries into the alveoli. • Page 35 of 114 . called the pleura. • Each lung is enclosed by a double-layered serous membrane. The small space between the visceral and parietal pleurae is the pleural cavity. which contains the heart. on the medial side. At the hilum. The only point of attachment for each lung is at the hilum. They are separated from each other by the mediastinum. and it helps to hold the two layers together as the lungs inflate and deflate. blood vessels. The fluid acts as a lubricant to reduce friction as the two layers slide against each other.
as a result move upwards and outwards. air is forced out. The diaphragm muscle contracts. This further increases the volume. This is known as inspiration. breathing out is a passive process (requires no energy) this is because the force of gravity and the recoil of elastic muscle fibres pull the rib cage downwards and inwards. Due to this. air must be constantly moved into and out of the lungs. Expiration Normally. The ribs. and is then lower than the air pressure outside of the lungs. The internal muscles contract while the external muscles relax.2 – The mechanism of breathing To maintain a steep concentration gradient. During expiration. Pulmonary Ventilation Pulmonary ventilation = tidal volume x ventilation rate (dm^3min^-1) (dm^3) (min^-1) Page 36 of 114 . There is internal muscles and external muscles. Air pressure in the atmosphere is greater than the air pressure in the lungs. Due to the increase in volume. The diaphragm relaxes and moves back into its domed shape. There are two types of intercostals muscles that lie between the ribs.Section 4. air is drawn in. When air pressure in the lungs is greater than the air pressure of the outside atmosphere. This further decreases the volume. the external muscles relax and the internal muscles contract. the external muscles contract. air is drawn in. the air pressure drops. The decrease in volume causes an increase in pressure and so air is pushed out. and flattens. the internal muscles relax whilst the external muscles contract. thus increasing the volume. this decreases the volume. During inspiration. The process of breathing is called ventilation. This is called expiration. Inspiration Inspiration is an active process (requires energy) In order to respire.
This brings haemoglobin very close to the alveoli air. The delicate surface area is protected from damage by being tucked away inside the chest. The lumen of the capillary is so narrow that the red blood cells slow down as the pass through it. This reduces the distance for diffusion. Ficks law Rate of diffusion = surface area of the membrane x difference in concentration length of diffusion path Having a vast number of capillaries is very important. Walls of the alveoli are very thin and close together. They are flattened against the alveoli.where gas enters and leaves the lungs.Section 4.3 – Exchange of gas in the lungs Gas exchange is the process by which 02 moves enters the blood and CO2 moves out. Diffusion occurs when there is a difference in concentration. Cellular respiration creates a constant demands for oxygen. This allows for efficient gas exchange. Page 37 of 114 . Single cell organisms can use there cell membrane as a surface for gas exchange. The movement of O2 is independent of the movement of CO2. Alveoli Mammals exchange respiratory gases mainly through the alveoli. CO2 will not diffuse out if the concentration is higher outside the lungs. Many organisms have developed specialised gas exchange structures called lungs. Cells in the alveoli wall are flattened with only a thing layer of cytoplasm between the cell membranes. Particles move down a concentration gradient. Gas exchange surface . Each alveoli is approximately 75 – 300 micrometers across. Breathing in air when there is a high concentration of CO2 can be lethal even when there is a rich supply of oxygen.
Endothelium is a specialised type of epithelium that lines the inner surface of blood vessels. The film of water slows down the rate of diffusion because it has increased the distance the gases need to travel. Epithelium and endothelium Epithelial cells – cells from epithelium tissue that lines the internal and external cavity. Alveoli must be kept open to increase their surface area. this is because the plasma membranes of its cells are permeable to water. Surfactant Surfactant prevents the alveoli from collapsing or sticking together.The inner surface of the alveoli wall is covered in water. For a membrane to be permeable to oxygen it must also be permeable to water. Alveoli structure The wall of the alveoli is made of epithelial cells. Lung surfactant reduces the surface tension so that the alveoli remain open. Page 38 of 114 .
TB is referred to as invasive because it enters and spreads into tissue. Can survive week disinfectants. Most commonly affects the lungs. Page 39 of 114 . It kills approximately 2 million people each year.4 – Pulmonary tuberculosis Tb is an infectious disease caused by an airborne. When a person coughs or sneezes. If the person has been exposed to the TB bacterium. You can only contract the disease after prolonged exposure. It is very resistant.Section 4. It is the biggest killer of women of reproductive age. TB is the leading cause of death from bacterial infection. People who are most at risk are: People who have HIV Those taking immune suppressant drugs Those under going cancer treatment The very young/old Those who live in LDCs Those who inject drugs or drink too much alcohol Skin test – the doctor would inject a very dilute extract of the bacterium into your skin. Divides once every 16-20 hours. The disease affects almost 1. Almost any part of the body can be infected by the pathogen (extra pulmonary tb). the immune response will cause an area of inflammation. Contracting TB Most people with tb only exhale a few bacteria in each breath. can survive several weeks in dry state. rod-shaped pathogen called mycobacterium tuberculosis. It has an extremely slow growth rate. The disease only develops if the bacterium reaches the alveoli. Droplets of MTB can remain suspended in air for several hours. causing pulmonary TB.7 billion people world wide. droplets of water are expelled and may contain the bacteria.
resulting in cavities and scar tissue where the lungs repair. The infection can lead to inflammation and enlargement of the lymph nodes responsible for that area of the lung. Page 40 of 114 . They can then affect other regions of the lungs. Symptoms Persistent cough Chest pain Coughing up blood Chill + fever Night sweat Loss of appetite Unexplained weight loss Fatigue Death – occurs when the sufferer has lost too much weight. other areas of the body can be infected. When you are most at risk When you are in regular contact with those who have the disease When your immune system is compromised. Immune system responds. Tb bacteria have a cell wall made of a complex. waxy material that protects it from the macrophages. The antibiotics are affective against most strains of the bacteria. The loss of S. bacteria are then engulfed by a type of white blood cell called macrophages which do not actually destroy the bacteria. If the bacterium enters the blood. Disease progression Your immune system kills the bacteria and no further symptoms are experienced.A can reduce the efficiency of gas exchange.Treatment MTB is a bacterium that can be treated with antibiotics. This is called active tuberculosis. Fluid collects in the lungs and breathing becomes difficult. Bacteria destroy the lung tissue. Most TB is curable using a combination of 4 different types of antibiotics. The drug is taken for 6 – 9 months. the bacteria could break out of the tubercles in the alveoli.
These bacteria are then engulfed by more macrophages and the cycle continues. if any symptoms and the infection is controlled within a few weeks. Active TB The bacteria can multiply within the macrophages and eventually cause the cell to burst. Page 41 of 114 . Lysosomes in the macrophages contain enzymes that break down the waste materials. releasing the bacteria.After 3 – 6 weeks another white blood cell called T-lymphocytes arrive at the site and activate the macrophages so they can destroy the bacteria. In a healthy person there are few.
Patients suffering from the disease cannot diffuse oxygen into their blood as efficiently.5 – Fibrosis. The lining of these airways become inflamed Goblet cells secrete more mucus. However. which causes the airways to become thickened. Diagnosed by a lung biopsy The fibrous tissue also reduces the elasticity of the lungs. Asthma Allergens cause a chemical called histamine to be produce. Chronic dry cough – bodies reflex to try and remove fibrous tissue. Often linked with occupation Most contaminants that reach the bronchi and brochioles are trapped in the mucus. They engulf bacteria and foreign particles. White blood cells near the alveoli are called alveoli macrophages. This makes it harder to ventilate the lungs. The muscles surrounding the bronchi and bronchioles contract Page 42 of 114 . silica and some gases Exposure to ionising radiation Autoimmune response to inhaling gas containing bacterial. the tissue is virtually irremovable. asthma and Emphysema Infection Can be caused by environmental agents such as asbestos. Fluid leaves capillaries and enters the lungs.. Pulmonary fibrosis Occurs when scars form on the epithelium causing them to become irreversibly thickened. Air laden with fine dust is drawn into the alveoli where there is no celia to sweep away the particles. fungal or animal products.Section 4. Shortness of breath – occurs due to the lack of oxygen diffusing into the blood as a result of the lengthened pathway and shallower concentration gradient. Pain and discomfort in the chest – caused by the pressure and damage caused by the tissue.
Difficulty breathing – due to constriction of airways A wheezing sound when breathing – caused by air passing through restricted airways A tight feeling in the chest – consequence of not being able to ventilate the lungs properly Emphysema In emphysematous tissue the elastin has become permanently stretched and the lungs are no longer able to force all the air out of the alveoli. Chronic cough – bodies reflex to try and remove damaged tissue. As a result. Bluish skin colouration – due to the lower levels of oxygen within the blood as a result of poor gas exchange Page 43 of 114 . the rate of diffusion is reduced and less gas exchange will take place. Shortness of breath – air cannot be ventilated as effectively. This causes the concentration gradient to become shallower.
It is divided into a left and right side by a septum. They need a circulatory system to move substances around the body. The pulmonary circulation pumps blood to the lungs to be oxygenated. Page 44 of 114 . Blood moves down pressure gradients. The heart produces the main pressure gradient. allowing them to move freely over each other. • Pericardium • • The heart is covered by a double layer of tough. Pericardium fluid is secreted by the membranes and reduces friction.Section 5. although contractions of skeletal muscles also push blood along veins. The human heart • • • Lies in the thoracic cavity Consists mainly of cardiac muscle Its pumping action ensures that fresh supplies of oxygen and nutrients are constantly being supplied to all living cells of the body. inelastic membranes which form the pericardium. The circulatory system • • • Mammals have a double circulatory system as blood passes through the twice on one complete circulation of the body. from high to low pressure.1 – The heart and heart disease • • • • Mammals are too large to rely on diffusion. The systemic circulation pumps oxygenated blood to every other part of the body that uses oxygen.
The right ventricle pumps deoxygenated blood through the pulmonary artery. The two upper chambers are called the atria and the two lower chambers are called the ventricles. Each side has two chambers. Left side of the heart • • • • The left atrium receives oxygenated blood from the pulmonary vein. Page 45 of 114 . to the pulmonary circulation. The ventricles pump blood into arteries. The left ventricle pumps oxygenated blood through the aorta into the systemic circulation. protects the heart. The pulmonary artery is the only artery to carry deoxygenated blood. anchors its surrounding structures and prevents overfilling of the heart with blood. The right side of the heart • • • • • The right atrium receives deoxygenated blood from the systemic circulation through the vena cava. the left side pumps oxygenated blood. Although the volume of blood they hold is the same. Ventricles • • The right ventricle pumps blood to the lungs where the left ventricle has to pump blood to the whole body. The pulmonary vein is the only vein to carry oxygenated blood. Ventricle walls are thicker than that of the atria as they have to pump blood over a greater distance. The atria receive blood from veins. Heart Chambers • • • • The right side pumps oxygenated blood. the left ventricle has a thicker muscular wall. Each atrium is elastic so it can stretch as it fills up with blood.• This sac. Atria have only a thin muscular wall as they only need to pump blood a short distance to the ventricle.
Thin tendons join to the edges of the valve flaps to the wall of each ventricle. Does not tolerate a lack of oxygen or nutrients and soon dies if its supply of blood is cut off. the valves shut. The pulmonary semi-lunar valve is between the right ventricle and the pulmonary artery. Page 46 of 114 . and one at the base of each artery leading from the ventricles. Valves in the heart are designed to open when there is high pressure forcing the blood on the correct direction. If high pressure forces the blood in the wrong direction. The tricuspid valve between the right atrium and the right ventricle has three flaps. The aortic semi lunar valve is between the left ventricle and the aorta. one between each atrium and ventricle. These arteries branch out to supply the thick heart muscle with oxygen and nutrients. they stop the valves turning inside out. unlike other muscles it never fatigues. Coronary arteries • Some of the bloody leaving the left ventricle goes to the coronary arteries. • The coronary arteries are much narrower than many other arteries and so can become blocked more easily. Cardiac Muscle • • A special type of muscle. Valves • • • • • There are 4 valves in the mammalian heart. How valves work • • • • • They prevent the back flow of blood. The bicuspid valve between the left atrium and the left ventricle has two flaps.• A thicker muscular wall will allow a stronger contraction to push blood further. These tendons to not stretch.
Both sides of the heart contract together. Blood returning to the heart fills the atria. This is known as diastole. When it is relaxing it is in diastole. Ventricle systole refers to the contraction of the ventricular myocardium.Section 5. forces the atrioventricular valves to open. Even though the atria aren’t contracting. Both semi-lunar valves are closed. The cardiac cycle is the sequence of stages that take place in one heart beat. Atrial systole • • • • • The myocardium of both atria contract. When a chamber is contracting it is in systole.2 – The cardiac Cycle • • • • The 4 chambers in the heart are constantly contracting and relaxing in a definite sequence. The stages of the cardiac cycle • • • • • There are three stages of the cardiac cycle: atrial systole and ventricular systole and diastole. Atrial systole refers to the contracting of the atrial myocardium (heart muscle). More blood passes through these valves into the ventricles. The higher pressure in the atria than the ventricles. pushing more blood into the ventricles. Page 47 of 114 . blood flows from the atria to the ventricles. This means that the atria will contract and relax at the same time and so will the two ventricles. Between heart beats the myocardium of both atria and ventricles are relaxed. The atrioventricular valves open. Diastole • • • Ventricular and atrial myocardium relaxes at the same time. This raises the pressure in the atria.
Pressure changes • • • The events of the cardiac cycle create pressure changes. Blood is pushed out of the heart into the pulmonary artery and aorta. stopping blood moving back into the heart. rather than by the nervous system. The semi-lunar valves close. The cardiac cycle starts at the sinoatrial node (SA node). Valves open or close when the balance of pressure on opposite sides of the valves changes. The SA node is a group of cells found out the top of the right atrium which acts as a natural pacemaker and initiates the heart beat. Both atrioventricular valves are forced closed When the pressure of the ventricles exceeds that of the arteries. • • • Page 48 of 114 .Ventricular Systole • • • • • • • • The myocardium of both ventricles contract The atria are relaxed The ventricles continue to fill with blood This quickly raises the pressure of the ventricles higher than that of the atria. Pressure changes are responsible for moving blood through the heart and into the systemic and pulmonary circulations. Myogenic contractions of the myocardium are largely responsible for the cardiac cycle. Controlling the cardiac cycle • Myogenic contractions are contractions originating from within the muscle. the pulmonary and aortic valves are forced open.
(av node) The AV node is another specialised group of cells. Bundles of his conduct electrical impulses rapidly down the atrioventricular septum. • • • • • Contraction of the ventricles • • • From the AVN two specialised bundles of purkinje tissue run down the atrioventricular septum and up the ventricular wall. The heart rate can also be controlled by nervous impulses and hormones such as during exercise and adrenalin.• • The rate at which the SA node produces the waves determines the heart rate. to the bottom of the heart. The cells in the AVN can conduct electricity but only shortly after a slight delay. This tissue conducts the impulses throughout the atria. from the base of the heart upwards. The contraction spreads outwards and downwards. These fibres stimulate the muscles of the ventricles to contract rapidly. This stops the waves of the atrial muscle contraction continuing through the ventricle muscles as the blood would be forced to the bottom of the heart. The heart beat Page 49 of 114 . from the top of the atria. The impulses are not carried by nervous tissue but by specialised muscle fibres called purkinje fibres. stimulating the myocardium of the atria to contract. Continuing the cardiac cycle • The electrical activity cannot pass from the walls of the atria to the walls of the ventricles. because it is stopped by a wall of fibrous tissue called the atrioventricular system. The delay allows time for the atria to complete their cycle. squeezing blood towards the ventricles. There is only one location where the impulse can travel from atrium to ventricle – through the atrioventricular node. Starting the Cardiac cycle • • • • The SA node produces waves of electrical impulses called cardiac impulses.
The cardiac output depends on two features: how quickly the heart is beating.• • First heart beat sound “lub” occurs when the atrioventricular valves close. Cardiac output • • • • • The volume of blood from ventricles in one minute. and the stroke volume (amount of blood in one beat). Measured in DM^3min^-1 The volume pumped by both ventricles pumped is the same. Cardiac output =heart rate (min^-1) X stroke volume (dm^3) Page 50 of 114 . Second heart sound “dub” occurs when the semi lunar valves close.
Blood pressure If the blood pressure in the arteries is high. it forms a rough surface that interrupts the otherwise smooth flow of blood. Athermanous plaques are made up of cholesterol.Section 5. thereby increasing blood pressure. fibres and dead muscle cells. glucose and other nutrients being transported to the tissue. Page 51 of 114 . but irreversibly with haemoglobin. blood filled structure called an aneurysm. Thrombosis If an Atheroma breaks through the endothelium of the blood vessel. otherwise known as a heart attack. If blood flow to the heart muscle is interrupted it can cause a myocardial infarction. the heart must work harder to pump blood into them. Aneurysm Atheromas that form thrombosis can weaken artery wall. Nicotine stimulates the production of adrenalin which will increase heart rate and blood pressure. Myocardial infarction Occurs when the hear stops beating. In order to supply tissue with the same amount of oxygen the heart must work harder. or athermanous plaque. Smoking Carbon monoxide combines easily.3 – Heart Disease Atheroma is the build up of fatty deposits that can impair blood flow. that will stop the flow of blood. Atheroma Begins as fatty streaks which are deposits of white blood cells that have taken up low density lipoproteins These streaks enlarge to form an irregular patch. The region of tissue deprived of blood due to the thrombus will not be able to respire as a result of no oxygen. causing them to swell to form a balloon like. thus reducing the oxygen carry capability of the blood. This may cause a thrombus (blood clot).
High blood pressure in the arteries means there is more chance of an aneurysm forming and bursting causing a haemorrhage. e. including the artery walls. which they infiltrate.g. Page 52 of 114 . High levels of saturated fat increase low density lipoprotein levels and hence blood cholesterol concentration. Foods that act as antioxidants. vitamin c. Diet High levels of salt raise blood pressure. and so does non-starch polysaccharide (dietary fibre). They help protect arteries against heart disease. Low density lipoproteins which transport cholesterol from the liver to the tissue. Blood Cholesterol High density lipoproteins remove cholesterol from tissue and transport it to the liver for excretion. restricting blood flow. To resist the high pressure the walls of the arteries tend to become thickened and may harden. leading to the development of Atheroma and hence a heart attack. reduce the risk of heart disease.
they would destroy the body’s tissue. Given that there are so many different types of lymphocyte in the body. but respond to all of them in the same way. Defence Mechanisms Non specific Response is immediate and the same for all Specific Response is slower and is specific to each pathogen pathogens Physical barrier Phagocytosis Cell mediated response.Section 6. There is over 10 million different types of T-lymphocytes.1 – Defence mechanisms Defence mechanisms Non-specific – mechanisms that do not distinguish between one type of pathogen and another. The response involves a type of white blood cell called a lymphocyte and can take two forms: A) cell mediated response (T-lymphocytes) B) Humoral responses (B-Lymphocytes) Recognising your own cells Lymphocytes must be able to distinguish between pathogens and the bodies own cells. • Page 53 of 114 . There are very few of each lymphocyte so response to an infection is slow. The response is less rapid but provides long lasting immunity. Tlymphocytes Humoral response Blymphocyte s • • • T-lymphocytes already exist within the body. If they did not. There is a high probability that when a pathogen enters the body the antigen on its surface will be complementary to a specific lymphocyte. These mechanisms act immediately and take two forms: A) barrier of entry B) phagocytosis Specific – Mechanisms that do distinguish between different pathogens.
The soluble products of the pathogen are absorbed into the cytoplasm of the phagocyte Page 54 of 114 . Phagocytosis • • • • • • Pathogens are engulfed by phagocytes in the form of vesicles which are formed on the cell-surface membrane. Hydrochloric acid in the stomach – Provides a low pH that denatures the pathogens enzymes.2 – Phagocytosis There are two different types of white blood cells.Section 6. creating a barrier that is hard for pathogens to penetrate. Barriers of entry • • • A protective covering – The skin covers the body’s surface. In the lungs pathogens are often caught in the mucus and moved by the cilia. The enzymes within the Lysosomes digest the pathogen. Phagocytes attach themselves to the surface of the pathogen. They engulf the pathogen to form a vesicle known as a “phagosome”. Phagocytes ingest and destroy pathogens by a process called phagocytosis. There are phagocytes and lymphocytes. Enzymes within the Lysosomes join with the phagosome and release their contents. Chemical products of the pathogen act as attractants which draw the phagocyte towards it. Epithelia covered in the mucus – Many epithelia produce mucus.
Body cells that have been invaded by a virus also manage to present some of the virus’ antigens on its surface as a sign of distress. D) This stimulates other T cells to divide rapidly by osmosis to form a clone. b) Stimulate phagocytes to engulf the bacteria by phagocytosis. This type of response is called “cell-mediated immunity”. T – Lymphocytes are associated with cell-mediated immunity i.3 – T cells and cell-mediated immunity Antigens An antigen is any part of an organism or substance that is recognised as foreign and stimulates a response from the immune system. immunity involving antibodies that are present in the body’s fluids. T-Lymphocytes only respond to antigens that are attached to a body cell.e. The hole then makes the cell freely permeable to all substances and quickly dies as a result. A) Pathogens invade body cells or are taken in by phagocytes. Instead.Section 6. c) Stimulate b cells to divide d) Kill infected cells T cells do not kill cells by phagocytosis. they produce a protein that makes a hole in the pathogen or infected cells. E) The cones T cells: a) Develop into memory cells that provided rapid response in the future.e. Page 55 of 114 . Lymphocytes B –Lymphocytes are associated with humoral immunity i. Antigens are normally proteins that are part of the organism’s cell-surface membrane. Both types of lymphocytes are formed from stem cells in the bone marrow. Cancer cells also present antigens on its cell-surface membrane. or “humour”. Cell-mediated immunity T – Lymphocytes can distinguish foreign material from the bodies own tissue because: Phagocytes have engulfed and broken down a pathogen and have presented some of its antigens on its own cell-surface membrane. C) Receptors on certain T helper cells fit exactly onto these antigens. immunity involving body cells. B) The phagocyte places the antigen on its own cell-surface membrane.
The surface antigens of the invading pathogen are taken up by the B cells. 1. This explains why it is possible to get the same diseases more than once. The B cells process the antigens and present them on their surface. The cloned plasma cells produce antibodies that exactly fit the antigens on the pathogens surface. T helper cells attach to the processed antigens and B cells thereby activating them.Section 6.4 – B cells and humoral immunity Humoral immunity is so called because it involves antibodies which are soluble in the blood and tissue fluid. Influenza for example has many different strains and so when a new strain enters the body. This is the primary immune response. and the ones they produce are constantly changing. These can respond to future infections by the same pathogen by dividing rapidly and developing into plasma cells that produce antibodies. The memory cells provide long term immunity. its antigens are not complimentary to the antigens or the memory cells produced from the last infection. Each B cell develops into two different types of cell: Plasma cells secrete antibodies directly. 4. Some B cells develop into memory cells. 5. This is known as the primary immune response Memory cells live for decades in some cases. Toxin molecules will also act as an antigen. The antibodies attach to antigens on the pathogens and destroy them. A typical pathogen may have more than one type of antigen on its surface. 2. 7. When they encounter the same antigen they divide rapidly into plasma cells and more memory cells. The B cells are now activated to divide by mitosis to give a clone of the plasma cells. This is the secondary immune response. 3. This is known as the secondary immune response. this is known as antigenic variability. They only live for a few days but produce more than 2000 antibodies every second. Antigenic Variability The antigens that pathogens are made of. 6. Page 56 of 114 . also called “humour”. Due to this the most use its primary immune response.
5 – Antibodies Antibodies are proteins synthesised by B cells.Section 6. Page 57 of 114 . Antibodies react with antigens by binding with them. Structure Antibodies are made up of four different polypeptide chains.
Section 6. Active immunity is produced by stimulating the production of antibodies by the individuals own immune system. Antibodies have a binding site that is very specific to the antigen once together they form and antigen-antibody complex The binding site is different for all antibodies and is known as the variable region. In is generally long-lasting. they are not being replaced when they are broken down in the body and so the immunity is generally short-lived. Monoclonal antibodies have a number of uses such as: The separation of a chemical from a mixture Immunoassay. Page 58 of 114 . The rest of the antibody is the same and is called the constant region. Each antigen will induce a different B cells to divide and clone its self. They will then activate a cytotoxic drug that will kill cells. while the other pair have shorter chains and are called light chains. Monoclonal antibodies A pathogen entering the body is likely to have hundreds of different antigens on its surface. Monoclonal antibodies can be used to knock out these T – Cells. testing for drugs in the urine.6 – Vaccination Passive immunity is produced by the introduction of antibodies into individuals from an outside source. Each clone will produce a different antibody known as a polyclonal antibody. and detecting the immunodeficiency virus (aids test) Cancer treatment – it is possible to manufacture monoclonal antibodies that will attach themselves to cancer cells. As the antibodies are not being produced by the individuals themselves. Antibodies that can be isolated and cloned are called monoclonal antibodies. Transplant surgery – Even with close matching the transplanted tissue will experience some rejection from the T – cells.The chains of one pair are long and are called heavy chains.this is the method of calculating the amount of substance in a mixture. This drug will only be activated by cells to which the antibody is attached. It is used in pregnancy testing kits.
Individuals may develop the disease immediately after vaccination but before their immunity levels are high enough to prevent it. These people have less effective immune systems and so vaccination is less effective at stimulating immunity. There must be the means of administrating the vaccine at the right time. Features of a successful vaccination program • • • • • • The success of a vaccination program depends on a number of factors: A suitable vaccine must be economically available in sufficient quantities There must be very few if any unpleasant side effects from the vaccine.Vaccination is the injection of a substance into the body with the intention of stimulating active immunity. Pathogens may mature rapidly so that their antigens change suddenly rather than gradually. Certain pathogens can hide from the body’s immune system by hiding themselves with cells or by living in places that are out of reach. storing and transporting the vaccine must be available. It must be possible to vaccinate the vast majority of the vulnerable population. Page 59 of 114 . The proportion of elderly people in the population is increasing. There may be many varieties of a particular pathogen. The antigens on the cholera surface change rapidly. The increasing amounts of people with HIV has led to more people having impaired immune systems and so are more likely to contract TB. The problems of controlling cholera and tuberculosis by vaccination • • • • Cholera is an intestinal disease and is not easily reached by the immune system. Means of producing. Why vaccination does not eliminate a disease • • • • • Vaccination fails induce immunity amongst some individuals. This is due to the antigenic variability.
If each member of a species differs from one another it is called intraspecific variation.1 – Investigating Variation If one species differs from another it is called interspecific variation.Section 7. Making measurements Page 60 of 114 .
be passed on to the next generation. all of which are different. To prevent sampling being biased. However. 2. Using numbers generated by a computer to obtain a series of coordinates 3. by pure chance be unrepresentative. Which gamete fuses with which at fertilisation are a random process which further adds variety to the offspring. Sampling – involves taking measurements of individuals. random sampling should be carried out instead. Causes of variation Genetic differences – due to differences in the genes of each individual organism Genetic differences occur due to: Mutations – sudden changes to genes and chromosomes may. selected from the population of organisms which is being investigated. Fusion of gametes – In sexual reproduction the offspring inherit some characteristics of each parent and are therefore different from both of them. Environmental differences In most cases variation is due to the combined effects of both genetic differences and environmental influences. either deliberately or unwittingly. Page 61 of 114 . This mixes up all the genetic material before it is passed into the gametes. or may not. Chance – Even if sampling bias is avoided. • • Sampling bias – The selection process may be biased. Take sample at the intersection of each set of coordinates. the individuals chosen may. Meiosis – Special form of nuclear division.Biologists often take measurements of some aspect of a living thing. all living organisms differ in some way. The affect of chance cannot be removed however it can be minimised by using large sample sizes and analysing the data collected at the end to determine the affect chance had and to what degree it influenced the data. As a result it is very difficult to draw conclusions about the causes of variation in any particular case. forms gametes. One method is to: 1. possibly due to the investigator making unrepresentative choices. Divide the study area into a grid of numbered lines.
Section 7. Variation can be represented on a bar chart of pie graph. Variation due to environmental influences Page 62 of 114 .2 – Types of variation Variation due to genetic factors Where variation is the result of genetic factors organisms fit into a few distinct forms and there are no intermediate types.
Page 63 of 114 . Add all the square numbers together and divide by the number of measurements. It does not however. do 6-9. it is the molecule of which our genes are made of. For example if the mean is 9 and one of the measurement values is 6. 4. to get the standard deviation. The mean provides an average value that can be used when comparing one sample with another. Section 8. Subtract the mean value from the measurement values. A standard deviation is the distance from the mean to the point where the curve changes from being convex to concave. provide any information about the range of values within the sample. 5. Environmental factors play a major role in determining where on the continuum an organism actually lies. Square root the number obtained in step 4. Square all the numbers obtained in step 2 to make them positive. Why is DNA important? Its structure enables it to play a key role in two essential features of all living organisms. 2.Some characteristics of organisms grade into one another.1 – Structure of DNA DNA – deoxyribonucleic acid It is a type of nucleic acid. If we take these data and plot them on a graph we obtain a bell-shaped curve known as a normal distribution curve. Standard deviation – Is a measurement of the width of the curve. Calculate the mean value (x bar). forming a continuum. 3. Mean and standard deviation Mean – measurement of the maximum height of the curve. Calculating standard deviation 1. It gives an indication of the range of values either side of the mean.
The pentose (5 carbon) sugar deoxyribose 3. They occur singly (mononucleotides). What is DNA made of? Each strand of DNA is made of repeated subunits called nucleotides. thymine. The bases are attached to the backbone. 2. Each chain is sing and unbranched These polynucleotide strands are twisted to for a double helix. in twos (dinucleotides). The phosphate and sugar molecule make the backbone of the DNA molecule. cytosine. The nucleotides of DNA Each nucleotide has three components: 1. What are nucleotides? DNA is called a polynucleotide. organic molecules that play a vital role in every organisms life. onto the next generation and protein synthesis. Nucleotides are joined together by a condensation reaction. Nucleotides are nitrogen containing. This forms a “sugar-phosphate backbone” with the base petruding outwards. DNA structure DNA molecules are huge: each molecule consists of two interconnected chains or stands. and the phosphate group of the next. A phosphate group The structure of the nucleotides The nucleotides in each strand of DNA are held together by the bonds between deoxyribose of one nucleotide. unaltered. or in thousands (polynucleotides). The reaction occurs between the deoxyribose sugar and the phosphate group.Inheritance – ensuring that DNA is passed on. Page 64 of 114 . An organic nitrogenous base: adenine. or guanine.
Bonding between DNA strands All the atoms in the nucleotides that make up each chain have no free covalent bond site. Individually. The hydrogen bonds are between the hydrogen atoms of a base in one chain and the nitrogen and oxygen atoms in another chain. C and T have a sing ring structure and are shorter. hydrogen bonds are weak. there four different types of nucleotides in a DNA molecule. Three hydrogen bonds are formed between G and C Two hydrogen bonds are formed between A and T Reason for pairing Hydrogen bonding only occurs between certain bases. Hydrogen Bonds Hydrogen bonds are weaker that phosphate covalent bonds. Each run on the ladder must be the same length so long molecules bond with short molecules. Base pairing rule If the nucleotide contains adenine it bonds with thymine. Hydrogen bonds are formed between the organic bases of each polynucleotide strand.The bonds linking the two nucleotides are called covalent phosphodiester bonds. Nucleotides containing cystine. Complementary bases Page 65 of 114 . The bond can be broken down by hydrolysis. G and A have a double ring structure and are longer molecules. bond with guanine. allowing strands to separate during replication and protein synthesis. DNA bases Adenine = A Thymine = B Cytosine = C Guanine = G As there are four different bases. but in a combination they can be strong. Hydrogen bonds break and reform.
A and T are complementary bases. the sequence of bases along a polynucleotide chain determines he sequence along another. Genetic code The order of bases in an organisms DNA The order of bases determines what amino acid is produced. DNA strands A polynucleotide has two distinct ends: a 3 prime end and a 5 prime end. carbon 3 of the deoxyribose is closest to the end. There are equal amounts of A and T and C and G DNA and variety What differs between DNA is the proportion and sequence of bases. Each of the two polynucleotide chains is anti-parallel. The Double helix DNA is similar to twisted ladder Each complete turn has ten base pairs Because of the complementary pairing. The four different types of nucleotides can join in an infinite number of ways. Page 66 of 114 . that is.2 – The triplet code Genes are sequences of nucleotides at a fixed position on a strand of DNA that specify a sequence of amino acids that form the primary structure of a protein. Section 8. they run in opposite directions. At the 3’ end.
forming polypeptides Genetic code summary Triplet – each of the 20 amino acids used to make proteins is represented by a base triplet in DNA. Some non – coding DNA is found within a gene. Page 67 of 114 . Amino acids Three bases can code for 64 different amino acids. As there are only 20 amino acids. Making proteins DNA codes for amino acids that form the primary structure of a protein. Non coding regions – introns Coding regions – exons Splicing The introns are spliced out by enzymes. We say the degenerate code. The remaining exons code for amino acids. Coding – non coding Much of the DNA is Eukaryotic cells does not code for polypeptides. Determining amino acid sequences Each is amino acid is determined by the sequence of bases in the gene. Each protein has a unique tertiary structure due to its sequence of amino acids. All proteins are made from the 20 amino acids.Each gene carries the code for a specific polypeptide. Non – overlapping: each base is part of only one triplet and is therefore involved in specifying only one amino acid. Joining amino acids Peptide bonds between amino acids. some found between genes. most amino acids have more than one code. Different numbers and sequences of these amino acids produce an almost limitless range of proteins. Each code has three bases – triplet code.
Is larger than prokaryotic DNA Page 68 of 114 . Some amino acids have more than one code. Section 8.Linear DNA – is always read from 5’ to 3’ Degenerate There are more base triplets than amino acids. Almost universal: the base triplet that codes for a particular amino acid in humans also codes for the same amino acid in most other living organisms.3 – DNA and Chromosomes Eukaryotic DNA • • • Is linear and forms chromosomes Found in the nucleus of cells.
Nucleosomes consist of a DNA molecules wrapped around a ball of 8 histone molecules.• • A single eukaryotic cell has many different molecules of DNA called chromosomes. Page 69 of 114 . The structure of polypeptides • • • • Composed of DNA and histones DNA and histones forms chromatin Nucleosomes are the basic structural unit of chromatin. Prokaryotic DNA • • • • Forms a closed loop and is circular. It is not organised into chromosomes. Mitochondria + Chloroplasts DNA does not have histones. Each chromosome is made of one DNA molecule Chromosomes are thicker and shorter than individual DNA molecules. therefore it is possible to see them under electron microscopes. ergo it does not need as many genes. This is because the cell is smaller and so is less complex. Found in the cytoplasm Found in a region of the cell called a nucleoid The DNA of prokaryotic cells is smaller than the DNA in eukaryotic cells. • • • Chromosomes • • • • • Human cells has 46 chromosomes (23 pairs) The chromosomes are only visible when the nucleus divides. Each chromosome carries the DNA for a large number of polypeptides. Does not contain non-coding DNA Many cells contain smaller circular pieces of DNA called plasmids.
Different genes are active in different cells. whereas the smallest contains approximately 300. Each gene has a specific position on a chromosome called its locus. Alleles are different version of the same gene. Genes come in more than one form. size and code for the same genes. Changes in the genetic code can mean that a particular protein is not produce. Homologous Chromosomes • • • • • • The members of each pair of chromosomes have the same shape. is not produced properly or is produced in the wrong amounts. Karyotyping • A karyotype is a photograph of a person’s chromosomes arranged in order of size. Chromosomes and genes • • • • The largest chromosome can contain approximately 8000 genes. the other from the father (paternal). Cells with pairs of homologous chromosomes – are called diploid cells. Cells with one chromosome from a homologous pair are called haploid cells.• Alleles • • • • Chromatin takes up special stains and is visible in a non-dividing nucleus. We call the members of each pair. One chromosomes comes from the mother (maternal). Gametes are haploid cells. Page 70 of 114 . homologous chromosomes. Chromosomes come in pairs and so there are two copies of the same gene. As we have two of each gene we have two alleles for each gene.
The other 22 chromosomes are called autosomes Karyotyping is used to spot chromosomal disorders Section 8.4 – Meiosis and Genetic Variation The division of the nucleus occurs in two ways: Mitosis – produces two daughter nuclei with the same number of chromosomes as the parent cell and as each other. Why is meiosis necessary? Page 71 of 114 .• • • Sex chromosomes are the 23rd pair. each with half the number of chromosomes as the parent cell. Meiosis – produces four daughter nuclei.
One of each pair will go into the daughter cell. At the end of this first stage. the alleles differ. The number of chromosomes in a gamete is a haploid number. Meiosis allows for genetic material from both the mother and the father to be passed on.When two gametes fuse to give rise to new offspring there chromosomes pair with each other. The chromatids of each pair twist around one another 2. the homologous pair would have separated. leaving just one chromosomes in the daughter cell. Variety from new genetic combinations Independent segregation brings about genetic variation because although the homologous chromosomes have the same genes. Crossing over occurs. Gene – section of DNA that codes for a polypeptide Locus – Position of a gene of a chromosome Allele – One of the different forms of a gene Independent Segregation of Homologous Chromosomes When homologous chromosomes line up. of these chromosomes produces different genetic combinations. the combination of chromosomes that go into the daughter cell is also random. therefore when they fuse the diploid number of the cell is restored. Because the chromosomes line up randomly. tensions are created causing portions of the chromatids to break off. Genetic recombination by crossing over 1. At the end of meiosis two there will be 4 new cells. where equal proportions of each chromatid are exchanged. and consequent independent assortment. Meiosis brings about genetic variation by independent segregation of homologous chromosomes and recombination of homologous chromosomes by crossing over. The random distribution. During the twisting process. The process of meiosis First Division – Homologous chromosomes pair up and their chromatids wrap around each other. they do so randomly. each chromosome separates into two chromatids. In humans each cell will have 23 chromatids. Page 72 of 114 . Each of which will enter the daughter cells. Second division – During the second division.
5. yet differ in terms of the two alleles they will posses for each gene. In this way new genetic combinations are achieved. 4. These portions of chromatids then join on to the homologous partner.1 – Genetic Diversity Genetic Diversity • • • • Similarities and differences amongst organisms may be defined in terms of the variation in DNA. This is due to the Page 73 of 114 . The greater the number of alleles for genes. Section 9.3. Hence it is the differences in DNA that lead to the vast differences in genetic diversity on earth All members of the same species have the same genes. the greater the genetic diversity within a species If there is a lot of genetic diversity within a species there is more chance that the species will be able to cope with changes to the environment. Usually equivalent portions of chromosomes are exchanged.
Offspring without desired characteristics may be killed or prevented from breeding Due to this. the genetic diversity of the population will be restricted. Each polypeptide is associated with a “haem” group (Fe2+) Page 74 of 114 . As these individuals become re-established. important for its ability to hold oxygen Quaternary Structure – All four polypeptides are chained to form an almost spherical model. Section 10. yet may be genetically distinct from the original population. The new group will show less variation. or artificial selection. Selective Breeding • • • • Selective breeding. alleles with unwanted characteristics are bread out of the population. there is therefore a greater probability that there are members within the species that have the characteristics required to adapt to a certain change in the environment.fact that because there are more alleles. Genetic Bottlenecks • • • Occurs when a species suffers a dramatic drop in numbers The few survivors will possess few alleles than the original population therefore there is less genetic diversity. involves selecting individuals with desired characteristics and using them to parent the next generation. The founder affect • • • • Occurs when a few individuals colonise a region The few individuals will possess fewer alleles and may not be representative of the whole population.1 – Haemoglobin Haemoglobin molecules Primary Structure – Consists of four polypeptide chains Secondary Structure – Chains are coiled into a helix Tertiary Structure – Polypeptide chain is folded into a precise shape. Selective breeding is used to produce high yielding breeds of domestic animals and strains of plants. In time this new population may become a separate species.
The role of Haemoglobin • • • • • • Transportation of oxygen Haemoglobin must be able to: Readily associate with oxygen at the surface where gas exchange takes place Readily dissociate from oxygen at respiring tissues Haemoglobin can change its affinity for oxygen under certain condition Its shape changes in the presence of certain substances such as carbon dioxide. should have haemoglobin with a high affinity for oxygen. An organism with a high metabolic rate needs to give up its oxygen more readily to tissues. and so haemoglobin with a low affinity for oxygen is necessary. its shape changes so that its affinity for oxygen decreases and so can dissociate from the oxygen molecule more readily. An organism living in an environment where there is little oxygen. therefore allowing a total of four O2 molecules to be carried. the Fe groups are close together.Each Fe ion can combine with a single oxygen molecule. haemoglobin may not absorb oxygen evenly.2 – Oxygen dissociation curves At different partial pressures. Section 10. thus making it easy to take up oxygen. the greater its affinity for oxygen Page 75 of 114 . thus making it difficult to absorb oxygen. This is provided that it has a low metabolic rate and therefore does not need to give up its oxygen as easily to respiring tissues. When carbon dioxide is present. This is provided that the organism lives in an oxygen rich environment. Once one oxygen molecule is absorbed. • The further to the left the curve is. Haemoglobin with a low affinity for oxygen – takes up oxygen less easily but releases it more readily. the polypeptide chains move away making it easier for more oxygen molecules to be absorbed The graph shows that a small decrease impartial pressure can lead to a lot of oxygen being dissociated. Why do organisms have haemoglobin with different affinities for oxygen? Different sequence of amino acid changes the shape of the molecules and there for its ability to hold oxygen. Why have different haemoglobins? Haemoglobin with a high affinity for oxygen – takes up oxygen more readily but releases it less easily. At low concentrations of oxygen.
it releases its O2 more readily to respiring cells. The haemoglobin molecule changes shape in such away that its affinity for oxygen is reduced. Starch is made up of long chains of alpha glucose molecules. transporting and unloading oxygen • • • • • At the gas exchange surface. the haemoglobin therefore has a lower affinity for oxygen. High pH changes the shape so that oxygen can be easily absorbed. CO2 is produced by respiring cells. In tissues.• The further to the right. there is a low concentration of CO2 because it is diffusing out of the blood.3 – Starch. thus lowering pH. the lower its affinity for oxygen The affects of Carbon dioxide concentration In the presence of CO2. it lowers the pH of the blood. At respiring tissue the level of CO2 is greater. When CO2 dissolves. thus allowing for O2 to be absorbed more easily. • Page 76 of 114 . This causes haemoglobins affinity for oxygen to increase. The curve therefore shifts to the right. and the curve shifts to the right. At the gas exchange surface. glycogen and cellulose Starch • • • Starch is polysaccharide that is found in many parts of plants in the form of grains. It is an important component of food and is the major energy source of most diets. The pH is higher due to low CO2 concentrations. Loading. haemoglobins affinity for oxygen is reduced. A low pH can reduce haemoglobins affinity for oxygen. High rate of respiration more CO2 produced lower pH greater haemoglobin changes shape oxygen unloaded more readily more oxygen available for respiration. Section 10. Large amounts are found in seeds as an energy store. Haemoglobin then releases its O2 into respiring cells. The main role of starch is energy storage. CO2 is constantly being removed. The chain is unbranched and winds into a coil making the molecule very compact.
4 . alpha glucose can easily be used in respiration. In animals it is stored as grains in the muscles and the liver. cellulose forms straight chains that run parallel to one another. Glycogen is found in animal cells. When glycosidic bonds form between these molecules the result is that the – CH2OH group on each beta glucose molecule alternates from being above and below the chain Rather than forming a coiled chain like starch.Plant cell structure Leaf palisade cells Function – Photosynthesis Main features of its function include: • Long. the chains are held tightly together. it is more readily hydrolysed. Although each hydrogen bond is on its own weak. When hydrolysed. because there are many hydrogen bonds. but never in plant cells. Cellulose is major component in plant cell walls. thin cells that form a continuous layer for photosynthesis • Numerous chloroplasts that are arranged in the most effective way for absorbing the maximum amount of light • A large vacuole that ensures that the cytoplasm and chloroplasts are at the edge of the cell Chloroplasts The main features of chloroplasts are: Page 77 of 114 . It is strong and prevents the cell from bursting due to osmosis. This produces fundamental differences in its structure. allowing more to be stored in a small space. Glycogen • • • • Glycogen is similar to starch however it is highly branched and made up of shorter chains. It achieves this by exerting and inwards pressure that stops the influx of water. Cellulose molecules are grouped together to form microfibrils which in turn make parallel groups called fibres. allowing for hydrogen bonding to occur.• • • It is insoluble and therefore does not cause osmosis to occur. Cellulose • • • Cellulose differs from starch and glycogen in that it is made up of beta glucose rather than alpha glucose. • • • Section 10. The reason for this is that in beta glucose the position of the –H and the –OH group is reverse. It is compact. Because it is made up of shorter chains.
The chloroplast envelope is a double plasma membrane that surrounds the organelle. It is highly selective in what it allows to enter and exit. The grana are stacks of 100 disc – like structures called thylakoids. Within the thylakoids is the photosynthetic pigment called chlorophyll. Some thylakoids have tubular like extensions that join up with thylakoids of adjacent grana. The grana are where the first stage of photosynthesis takes place. The stroma is a fluid –filled matrix where the second stage of photosynthesis takes place. Within the stroma are a number of other structures such as starch grains.
Chloroplasts are adapted to their function in the following ways The granal membrane allows a large surface area for the attachment of chlorophyll, electron carriers and enzymes that carry out the first stage of photosynthesis. These chemicals are attached the membrane in ordered fashion. The fluid of the stroma possesses all the enzymes required for the second stage of photosynthesis. Chloroplasts contain both DNA and ribosomes so they can quickly manufacture some of the proteins needed for photosynthesis. Cell wall • • • Contains microfibrils of cellulose Consist of a number of polysaccharides such as cellulose There is a thin layer called the middle lamella, which marks the boundary between adjacent cell walls and cements cells together.
Functions of cellulose cell wall are: • Provides strength and prevents cell from bursting due to osmotic gain. • Mechanical strength to plant as a whole • Allows water to pass along it and so contributes to the movement of water through a plant.
Section 11.1 – Replication of DNA
Why is DNA replication needed? • • • • • Multi-cellular organisms are constantly loosing cells which need to be replaced. More cells are also needed when the organism grows. Extra cells are only produced when the parent cell divides. New cells must contain the same genetic information as the parent cell. To achieve this, DNA must be able to replicate its self exactly.
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Following the replication of DNA, a chromosome appears to double its structure composed of two chromatids. The chromatids are identical as the DNA in the original chromosomes has replicated exactly. The two chromatids are temporarily held together by a centromere. The replicated chromosomes has twice the DNA as the regular chromosomes, but still only counts as being one chromosome. The chromatids become separated when the cell divides. Stages of DNA replication Stage 1 Strands of DNA separate The double helix structure of the DNA molecule partially unwinds An enzyme called Helicase breaks hydrogen bonds between complementary basis. The two strands are now separate Stage 2 Free nucleotides bond with the complementary basis exposed on each poly nucleotide. Large numbers of nucleotides are made in the nucleus and are attracted to the exposed basis. Hydrogen bonds form between complementary basis and as a result the strand builds up into a sequence of nucleotides. Stage 3 Nucleotides bond together Bonds have already formed between bases. Through condensation reactions, an enzyme called DNA polymerase forms covalent phosphodiester bonds between the deoxyribose of on nucleotide and the phosphate group of the next. The DNA molecule rewinds into a double helix and the process is complete. Leading and lagging strands Nucleotides can only be joined together in the 5’ to the 3’ On the leading strand, nucleotides can be added continuously in the same direction as the movement of the replication fork. On the lagging strand, continual synthesis is not possible, so small fragments of DNA are constructed. These fragments are linked together by DNA ligase. Origins of replication – replication forks
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The origins of replication are the points where replication begins DNA molecules are very long and so it would take too long if it started at one end and moved along the molecule. Instead, the double helix opens up and is replicated at a number of different sites. Replication forks resemble branching prongs where DNA Helicase is separating DNA into single strands. Semi conservative replication Each DNA molecules is formed from an intact strand from the original DNA and are newly synthesised. Both new DNA molecules are identical to one another and to the original molecule. Evidence for DNA replication Intact DNA acts as a template Mixture of nucleotides DNA polymerase ATP for energy New DNA molecules were formed that contained the same proportions of bases. Strong indication that DNA can copy its self by base pairing Alternative to semi-conservative The conservative model: parental DNA remains intact and the new molecule is built from completely new material. The dispersive model: proposes the new DNA molecules consist of sections of both old and new DNA, interspersed along each strand.
Section 11.2 – Mitosis
When a cell divides to make new cells, the DNA must be copied exactly into each cell. To do this, the parent cell divides by mitosis Why are new cells needed? For growth of a zygote into a multicellular organism
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A specific sequence of DNA bases that is not transcribed. The centromere The centromere is a constricted region of the chromosome that is made up of two components.Differentiation and repair: worn out or damaged cell tissues must be replaced by cells that do the same job. Prophase By the start of prophase. therefore each daughter nucleus contains exactly the same amount and the same type of DNA as the other daughter cell and parent nucleus. DNA has replicated and so chromosomes have a double structure. The chromosomes that were single stranded become a double structure. each chromatid in the two chromatids that make up a chromosome are called sister chromatids. Chromatids are held together by a centromere. Asexual reproduction: offspring are genetically identical to the parent. Page 81 of 114 . but which is required for the segregation of chromatids A protein based structure called a kinetochore to which spindle fibres attach Mitotic cell division Two main stages Division of nucleus. Each structure is chromatid. mitosis Cell divides cytokinesis Mitosis is a continuous process although it is described in 4 stages. Each chromosome contains one molecules of DNA. Daughter cells Mitosis results in each daughter nucleus having the same number and same type of chromosomes as the parent nucleus. such as in bacteria amoeba and non flowering plants. DNA replication is needed Daughter nuclei are identical because the DNA replicates. function and genetic information as the original cell they are replacing. Therefore the new cells must have the same structure.
Cytokinesis • • • • Cytokinesis is when the plasma membrane forms a constriction across the centre of the cell. The original nucleus has divided into two genetically identical daughter nuclei. Page 82 of 114 . BY the end of anaphase there are two groups of chromosomes. Chromatids are now referred to as chromosomes. The result is two daughter cells. Next the cell must divide into two. • • • • Sister chromatids . This becomes narrower and narrower finally dividing the cytoplasm into two cells. One at each pole Each group contains one chromatid from each pair of sister chromatids. forming chromosomes that are tightly coiled and appear shorter and thicker. Anaphase • • • • • Involves splitting of the centromere The separation of the chromatids Spindle fibres pull chromatids to opposite poles of the cell. As soon as the chromosomes condense. Nucleoli appear in each daughter nucleus. Telophase • • • • Nuclear envelope reforms around daughter nucleus.The chromatin condenses. Telophase marks the end of mitosis. Chromosomes become long and thin until they’re a mass of chromatin fibres and can no longer be seen with a microscope.attach to the centromere Nuclear envelope breaks down Spindle apparatus appear Nucleolus disappears Metaphase • • • Chromosomes align at the centre of the cell Spindle fibres appear from the poles of the cell and attach to the centromere One sister chromatid of each chromosome is attached by spindle fibres to one pole the other is attached to the other pole. the DNA becomes inactive. Some cells such as those of the muscles divide their nuclei without cytokinesis.
forming the two new cell walls and separating the two daughter plant cells. Page 83 of 114 .Cytokinesis in plants In plant cells.3 – The cell cycle The cell cycle is the sequence of events that take place in dividing eukaryotic cells. In actively dividing eukaryotic tissues. Key features of mitosis • • • • Only one nuclear division Two daughter cells formed Daughter cells are diploid Daughter cells are identical Section 11. The 2 events covered in already are only short parts of the cell cycle. This grows outwards and fuses with the cell wall. a cell plate is formed in the centre of cells. the new cells formed by mitosis grow before replicating their DNA and dividing by mitosis again.
Binary fission is different because it cannot be divided into phases because prokaryotes do not have a nucleus and a centromere. The first 3 phases (G1 S and G2) are often grouped together and called interphase. therefore must enlarge and synthesis new organelles. If it is not. Chromosomes are now duplicated The amount of DNA remains at this “double level” until cytokinesis because until then the DNA is contained within the cell. Each part of the cell cycle involves specific cell activities. A cell that has formed due to cell division is initially have the size of the parent cell with only half the number of organelle as the parent cell. billions of red blood cells are replaced each day and are lost from the surface of the skin. a non embryonic cell goes through four main phases then divides by cytokinesis.Prokaryotic cell division Prokaryotes do not divide by mitosis but instead by binary fission. Page 84 of 114 . G2 phase Organelles are replicated Proteins are made to create spindle apparatus that will separate chromosomes during mitosis and synthesis. S phase – synthesis of DNA DNA is replicated and combines with newly formed histones to double the amount of chromatin in the nucleus. The four main phases Once in the cell cycle.The cell continues to grow in size. The 4th phase is mitosis. Why do cells need replacing? Cells are constantly being scraped off of the lining of the gut. Phases of the cell cycle G1 phase of interphase The cell is active growing and increases in size Nucleotides and histones are produced Proteins are produced from which organelles will be synthesised Cellular checks are made to ensure that the DNA is in good enough condition to be replicated. M phase Chromatids are separated and go into daughter nuclei The nucleus divides before cytokinesis The cytoplasm divides to form new cells. The daughter cells are genetically identical to the parent cell. the cell is terminated.
In humans. Cancer cells are damaged the most as they are the fastest dividing. Sometimes the protective mechanisms break down and rogue. Treatments work by blocking some part of the cell cycle. Chromosomes must be treated with a stain to be made visible. The duration of the cell cycle varies greatly from organism to organism and from cell to cell. Cancer and the cell cycle Most mutated cells that are undergoing unwanted cell division are destroyed by phagocytes. Cancer treatments Effective cancer treatments require early diagnosis. DNA replication is generally faster in simpler organisms with smaller genome. Section 12. the whole cycles takes approximately 24hours How to prepare slides Onion roots are often used as their cells are rapidly dividing and so the will be many cells in different phases. Cancer drugs target all rapidly dividing cells that have a short or absent G0 phase. formed during fertilization when a sperm and egg nuclei fuse. causing cells to enter the cell cycle when there is no need to control growth/repair. Problems with treatments Cancer treatments do not just stop cancer cells. The tips must be squashed so that they form a thin single layer that easily allows the light to pass through it. Mitosis and cancer Most cells only divide by mitosis when required to do so. mutated cells remain in the cell cycle. The process is carefully controlled by genes Eukaryotic cells normally divide when triggered to enter the cell cycle by one or more chemical factors. Page 85 of 114 . Non-dividing ells are not considered to be in the cell cycle – G0 phase. Nerve cells are permanently in G0 phase Genes controlling cell division might mutate.Length of the cell cycle The length of the cell cycle is important because it determines how quickly an organism can multiply or grow or replace damaged cells. These cells continue to divide out of control and form a malignant tumour or cancer.1 – Cellular organisms Making a multicellular organism All organisms start life as a zygote.
They can develop into and of the 200 different cells that make up the human body. Epithelial tissues: Lines the body surfaces. found amongst differentiated cells in a tissue or organ that can its self and can differentiate to yield some or all of the major specialised cell types of the tissue/organ. The primary roles of an adult stem cell in a living organism are to maintain and repair the tissue in which they are found. One a cell becomes specialised it is usually unable to make other types of cells. Cells in different positions in an embryo develop in different ways to form tissues. Because different genes are expressed. the rest are switched off. These inner cells are called stem cell and will eventually give rise to nearly all the different adult cells. then four.The cell goes through a series of mitotic cell cycles to form two cells. Adult stem cells An adult stem cells I thought to be an undifferentiated cells. Because they can develop into any tissue. Specialised cells They have been differentiated They have lost the ability to carry out other functions. Connective tissue: adds support and strength to the body. but those taken from the blastocyst are called embryonic stem cells. These adult stem cell generally remain inactive until needed. Stem cells become specialised when the genes required for a particular function are switched on and all others are switched off. Muscle tissue: moves the body or body parts. Page 86 of 114 . This resulting in a multicellular organism with cells genetically identical to the original single cell Cells formed during early mitotic division form a hallow ball of cells known as a blastocyst A blastocyst consists of an inner cellular mass surrounded by a layer of cells. Embryonic stem cells There are different types of stem cells with varying abilities of differentiation. Not all genes work in all cells – only a few genes are switched on I any one cell. Injection of stem cells could hopefully lead to regeneration of healthy tissues. etc. Nerve tissues: enables quick communication between body parts. this determines the size and the shape of the cell and how many of each organelle are produced. the surfaces of organs or organ cavities and tubes. Forming different cell types All of out cells have the same DNA but they differ in how these instructions are used. embryonic stem cells have the ability to be used in the treatment of degenerate diseases and growing new organs.
This will therefore influence the type of exchange surface and transport system that has evolved. kidneys. Each tissue performs its own function and is essential to the overall function of the organ. Organ system An organ system is comprised of two or more different organs working together to a common function.1 – Exchange between organisms and their Environment The size and metabolic rate of an organism will affect the amount of each material that needs to be exchanged. other organs include the heart. The skin is the largest organ within the human body. etc. Materials that are exchanged between the organism and the environment include: • Respiratory gases Page 87 of 114 .Organs Organs are structures within an organism that are made of at least two types of tissue. Section 13. liver.
Actively – Energy is required (Active transport) For exchange to be affective. vitamins and minerals) Excretory products (urea. Organisms have evolved in the following ways so that they can provide all their cells with the material necessary in an affective manor: • • A flattened shape means that no cell is ever far away from the exchange surface (e.g.g.A: Vol. air) Movement of internal medium to maintain concentration gradient (e. fatty acids.• Nutrients (glucose. Very thin so that diffusion distance is short Partial permeable so that only certain materials can diffuse across Movement of the environmental medium to maintain concentration gradient (e. ratio and so gases exchange through their cell surface membrane.A to Vol. a flat worm) A specialised exchange surface. amino acids.g.celled organism have large S.2 – Gas exchange in single celled organisms and insects Gas exchange in single celled organisms Single . Diffusion is proportional to surface area X difference in concentration gradient Length of diffusion Section 13. Passively – requires no energy (diffusion. osmosis) 2. Features of specialised gas exchange surfaces Exchange surfaces have the following characteristics: • • • • • Large surface are to volume ratio to increase the exchange rate. and therefore increases the S. ratio. carbon dioxide) Exchange can happen in two ways 1. for example the lungs which has a large surface area. the surface area to volume ration of the organism must be high. blood) Diffusion can be explained with fick’s law. Page 88 of 114 .
For diffusion to be affective the pathway needs to be short. terrestrial organisms usually have the following two features: Water proof coverings over their body surfaces.e.3 – Gas exchange in Fish Fish have specialised gas exchange surface – the gills. Spiracles are often closed to prevent water loss. i. Gas exchange in insects Terrestrial insects have a problem where they loose water through the surface of their bodies and so easily become dehydrated. In the case of insects this is a waterproof cuticle. Small surface area to volume ratio to minimise the area over which water is lost Insects gave developed an internal system of tubes called trachea The trachea has strengthened rings for support. This sets up a diffusion gradient where oxygen in the atmosphere moves towards where there is less oxygen. • Along diffusion gradient – During respiration. To inhibit water loss. The trachea branches into smaller tubes called tracheoles. Gases enter and leave the trachea through small pores called spiracles. the cell wall does not affect the diffusion of gases as it is completely permeable. This speeds up gas exchange as it maintains a diffusion gradient between the two mediums. • • • • • Section 13. Ventilation – The movement of muscles in insects cause mass movements in air into and out of the trachea. the tracheoles. Gases move into and out of the respiratory system in the following ways. Structure of the gills Page 89 of 114 . Spiracles open periodically to allow gas exchange.For cells that have a cell wall. oxygen at the end of the tracheoles is reduced. Carbon dioxide is also produced during respiration which sets up a diffusion gradient that moves in the opposite way. This has limited the size of insects.
which has its maximum concentrations of oxygen. In the same way. Therefore diffusion from water to blood takes place. Water is taken in through the mouth and forced over the gills. some Page 90 of 114 . Due to the counter current flow approx 80% of the available oxygen is absorbed. although some carbon dioxide comes from respiring cells.4 – Gas exchange in the leaf of a plant When photosynthesis is taking place. There is a consistent rate of diffusion from the water to the lamella. At right angle are structures called lamellae which increase the surface area for gas exchange. Blood with little to no oxygen meets water with most of its oxygen removed. The counter current flow • The counter current flow ensure that there is a concentration gradient maintained between the two mediums so that gas exchange can take place at a fast rate. However oxygen diffuses from water to the gills regardless.• • • • • They made up of gill filaments Gill filaments are stacked up in a pile. Without it the maximum amount would be 50% as there would be no diffusion gradient. Blood flows in the opposite direction to the water that moves over the gills. • • • • Section 13. This is known as counter current flow. most of it needs to be taken directly from the air. Blood that is already well loaded with oxygen meets water.
Due to this. oxygen diffuses into the leaf because it is constantly being used by cells during respiration. interconnecting air spaces that occur throughout the mesophyll Stomata • • • • • Stomata are pores which occur mainly but not exclusively on the lower epidermis of the leaf. flat shape that provides a large surface area Many small pores called stomata. When photosynthesis is not taking place. they have the ability to control the rate of gaseous exchange. which makes it more rapid than if it was in water. This means they are able to prevent water loss Section 13. Om the same way carbon dioxide produced by respiration diffuses out. Diffusion takes place in the gas phase. Structure of a plant leaf and gas exchange No living cell is far from the external air. There is a short diffusion pathway. Each stoma is composed of two specialised cells called “guard cells”.oxygen from photosynthesis is used in respiration but most of it diffuses out of the plant. Numerous. Guard cells can open and close the stomatal pore. No specialised transport system is required for moving the respiratory gases in leaves. Leaves have the following adaptations for gas exchange: • • • A thin.5 – Circulatory system of a mammal Page 91 of 114 . which are found mostly in the lower epidermis of the leaf. and therefore a source of oxygen and carbon dioxide.
Why large organisms need a transport system A transport system is required to take materials from cells to exchange surfaces and from exchange surfaces to cells. Features of a transport system • • • • • • • Common features include: A suitable medium for carrying materials e.g. valves A means of controlling the transport medium Transport system in mammals Mammals have a double circulatory system which means blood travels twice through the heart I one complete circuit. ratio and how active the organism is. They also need to be transported to different parts of the organism. The final phase where materials pass from the blood to the cells and vise versa is rapid because it takes place over a large surface area. Materials have to be transported from the environment to cells. Whether or not a specialised transport system is required depends on the S.g. across short distances and down a steep concentration gradient. allowing it to travel around the body. A mechanism to maintain mass flow in one direction e. By going back to the heart to be pumped once more. Substances are quickly delivered to the rest of the body. Although transport systems move materials a over longer distance. Page 92 of 114 . blood because it is water based and so substances can dissolve. a specialised transport system is required.A to vol.Diffusion is only adequate for the movement of substances over a short distance. A form of mass transport by which the medium is moved A closed system of tubular vessels that contains the transport medium A mechanism that moves the transport medium creates a pressure difference. In order to move substances a long distance. which is necessary as mammals have a high metabolic rate. When blood travels through the lungs is pressure is reduced. its pressure increases. the final part involves diffusion into cells. With out going back to the heart the pressure would be low and so blood would take longer to circulate the body.
• • Arteriole structure and function • • Muscle layer is thicker than that of arteries – contraction of muscle layer narrows the lumen and so constricts blood flow and helps control the blood flow into capillaries. Page 93 of 114 . Capillaries – tiny vessels linked to arterioles 4. Arteries – carry blood away from the heart 2. Vein structure related to function • Muscle layer is thin – constriction does not control the movement of blood into capillaries because blood is moving away from tissues. Thickness of the wall is large – prevents vessel from bursting There are no valves – Blood is under constant high pressure and does not move back. Veins – Carry blood back to heart Arteries. The wall stretches (systole) then recoils back (diastole).Section 13. arterioles and veins have the same basic structure which includes: • A tough outer layer .6 – Blood vessels and their functions Structure of blood vessels There are 4 different types of blood vessels: 1. Stretching and recoiling helps maintain pressure and creates smooth pressure surges. Arterioles – smaller arteries that control blood flow to capillaries 3. Elastic layer is thinner than that of arteries – The pressure in arterioles is lower than in arteries.resist pressure changes • A muscular layer – Contract to control blood flow • Elastic layer – maintains blood pressure by stretching and springing back • Endothelium – smooth to prevent friction and thin to increase diffusion • Lumen – central cavity through which blood flows Artery structure and function • • Muscular layer is thicker than veins – smaller arteries can be constricted and dilated to control blood flow Elastic layer is relatively thick compared to veins – Pressure in arteries is high so that it can reach extremities.
This creates a hydrostatic pressure at the arteriole end of the capillaries. • • Hydrostatic pressure outside of capillaries Lower water potential of the blood due to its contents e. it allows veins to be flattened thus aiding blood flow There are valves throughout – prevents back flow of blood because pressure is low. Also.• • • Elastic layer is relatively thin – pressure in veins is low and so the blood vessel will not burst. This is called ultra filtration Return of tissue fluid • • • • Loss of tissue fluid in capillaries reduces hydrostatic pressure. When body muscles contract. The content of the lymphatic system is moves by hydrostatic pressure of tissue fluid leaving capillaries. proteins causes water from tissue fluid to move back into the capillary. Valves ensure that the pressure directs blood toward the heart. Hydrostatic pressure forces tissue fluid out of the blood plasma. and contraction of body muscles that creates pressure changes. then arterioles then finally narrower capillaries. Tissue fluid is then forced back into the capillaries. The overall affect means that there is only a small pressure that pushes small molecules out of the capillaries. veins are compressed pressurising blood within them. This however is apposed by two forces. Page 94 of 114 . In addition the pressure is to low to create a recoil action The overall thickness of the wall is small – No need for the wall to be thick as the pressure within veins is small. Valves also ensure the fluid moves in one direction. Some tissue fluid travels back via the lymphatic system. Capillary structure and function Walls consist only of lining layer – short diffusion pathway Numerous and highly branched – increased surface area Narrow diameter – permeate tissues Narrow lumen – pushes red blood cells which reduces diffusion pathway There are spaces between the lining – allows white blood cells to escape Tissue fluid and its formation Blood pumped by the heart passes through arteries. When blood reaches the venous end its hydrostatic pressure is less than that outside of the capillary.g. Osmotic forces from proteins in the blood pull water back.
This is a result of waters cohesive properties due to it being a polar molecule. The water then moves by osmosis. The symplastic pathway Water moves through the cytoplasms of cells of the cortex by osmosis. The water potential in root hair cells is very low due to the sugars and amino acids dissolved in them. • They have a thin surface area. Plants are always loosing water by transpiration and so this must be replaced. Page 95 of 114 . Water moves through small pores called plasmodesmata. and therefore short diffusion pathway. • Water then moves via osmosis to the first cell of the cortex. which has a much higher water potential to move into the cell by osmosis. They are efficient exchange surfaces because: • They have a large surface area for rapid diffusion of water. Water moves through the plant by the following pathways: • The apoplastic pathway • The symplastic pathway The apoplastic pathway • • As water is drawn in through the endodermal cells. The water moves through the cellulose cells walls. They absorb water and mineral ions. it pulls more water along with it. The process happens in the following way: • Water enters by osmosis and increases the water potential of the root hair cell. • This cell then has a higher water potential than the neighbouring cell. this causes the water in the soil. further into the root. thin extension of the root epidermal cell. Each root hair is a long.7 – Movement of water through roots Uptake of water by root hair cells Root hair cells are the exchange surface of plants.Section 13.
for example Cyanide stop active transport and therefore cause root pressure to cease. • Metabolic inhibitors. Water moving into the xylem via osmosis creates a force called root pressure. Passage of water into xylem Water travelling by the apoplastic pathway eventually reaches the Casparian strip where it is prevented from travelling further by the waterproof band. This is explains why water from the apoplastic pathway is forced into the cytoplasm of cells by the Casparian strip. • • Active transport of salts in the most likely way by which water moves into the xylem Endodermal cells actively transport salts into the xylem which sets up a water potential gradient.• A water potential gradient is set up along the cells of the cortex. If water in to move into the xylem it must first enter the cytoplasm of endodermal cells. Page 96 of 114 . Water is then forced into living cells where is joins water travelling via the symplastic pathway. • • • Evidence of root pressure includes the following: • The pressure increases with a rise in temperature and decreases with a fall in temperature. this carries water through the cytoplasm of cells to the endodermis. • A decrease in oxygen for respiration decreases root pressure.
water enters by osmosis. Water that leaves the stomata via evaporation is replaced by water evaporating from the cell wall of mesophyll cells. Change in diameter of tree trunks in relation to the rate of transpiration. there is negative pressure in the xylem and so the trunk shrinks. Movement of water across the cells of a leaf Water that evaporates from mesophyll cells is replaced by water reaching the mesophyll cells from the xylem through the apoplastic/symplastic pathway. Page 97 of 114 . When transpiration rate is high. This is called transpiration pull. the water movement occurs because: • Mesophyll cells loose water to the air spaces • These cells now have a lower water potential. • The loss of water from neighbouring cells lowers their water potential • They then take water from their neighbouring cell Movement of water up the stem through the xylem Movement of water up xylem is due to root pressure and cohesion tension Cohesion theory operates as follows: • Water leaves as a result of transpiration • Due to hydrogen bonds.8 – Movement of water up stems Water leaves plants by evaporating and moving out through the stomata Movement of water through stomata When the stomata are open. water molecules stick together. water molecules are drawn up behind it. Plants can control there rate of transpiration by opening/closing stomata. water molecules diffuse out into the surrounding air. Evidence to support cohesion theory includes: 1.Section 13. • Water is pulled up due to transpiration. For the symplastic pathway. • Water forms a continuous unbroken pathway across mesophyll cells and xylem • As water evaporates.
if there is little air movement there is likely to be a smaller water potential gradient and therefore slower transpiration rate. water can no longer be drawn up. so the stomata are open when there is a high light intensity (leaves photosynthesising) and close when there is a low light intensity (leaves not photosynthesising). To summarise this. Factors affecting transpiration Light Carbon dioxide diffuses in to leaves through the stomata so that it can be used in photosynthesis. Section 13. No additional metabolic energy is required. If air is being moved away from the leaf by wind a concentration gradient is maintained.2. 3. hence evaporating. water does not leak out. water would not be so plentiful and the transport of materials would not be so rapid. and so water evaporates at a slower rate because the osmotic gradient is decreased.9 – Transpiration and factors affecting it The role of transpiration Materials such as minerals and ions are moved around the plant dissolved in water This water is carried up the plant by transpiration pull Without transpiration. A rise in temperature decreases the amount of water air can hold. a water potential gradient is maintained and so transpiration occurs quicker. Humidity When humidity is high (when there is a large amount of water in a given volume of air) the water potential of the air is high. When there is a lot of air movement. In addition xylem vessels are composed of dead cells. Temperature Temperature affects the water potential of air and how fast water molecules move An increase in temperature increases the kinetic energy of water molecules. Transpiration pull is a passive process. Rise in temperature. These molecules will then have enough energy to change from its liquid state to its gaseous state. If a xylem vessel is broken and air enters it. Photosynthesis takes place during the day. rise in transpiration rate. This is because the continuous chain of water molecules joined by cohesion is broken. Air movement Water that has evaporated accumulates around the leaf and so the water potential gradient is decreased. When the xylem is broken. Page 98 of 114 .
This lowers the rate at which water is lost. This is due to the fact that the sun provides the heat energy and the light. contradict the plants ability to retain water. the energy that drives it comes from the sun.10 – Limiting water loss in plants The adaptation of an efficient gas exchange surface. Xerophytic plants Plants that have adapted to limit water loss are called. “xerophytes”. Section 13. Hairy leaves – hairs on the lower epidermis trap moisture in the air and so reduce the water potential gradient inside and outside of the leaf. The thicker the cuticle. both of which are factors affecting transpiration rates. Xerophytes have a thicker cuticle. thus lowering the rate of water loss. but also store and increase the uptake of water also. Once the water potential is the same as within the leaf. Stomata in pits and grooves – These just like the hairs on the lower epidermis of leaves trap air and so decrease the water potential gradient inside and outside of the leave. This creates a region of air that gets saturated with water molecules and increases in water potential.Although transpiration is thought of as a passive process. and so plants have adapted in other ways to limit water loss. the surface area to volume ration can be considerably be reduced. there is no net movement of water out of the plant. To compensate for this. A reduced surface are to volume ratio of leaves – by having leaves that are small and roughly circular in cross section. Xerophytic plants have adaptations to not only limit water loss. the lower the amount of water loss Rolling up of leaves – Leaves can wrap up so that their lower epidermis is enclosed within the leaf. This however contradicts the Page 99 of 114 . Examples of these modifications include: A thick cuticle – Plants that have leaves with a waxy cuticle can still loose water through the upper epidermis.
Natural classification Attempt to group organisms according to their natural relationship Reflects the way in which different groups are thought to have evolved Classifies species into a hierarchy in which smaller groups are contained within larger groups with no overlaps. If an organism is fertile it is capable of producing offspring. and that are reproductively isolated from other species. To classify all the organisms that w know about. Occasionally two different species can interbreed to produce organisms known as hybrids. The we use is based on dividing living organisms into species. Relationships are based on the homologous characteristics. The parents of the hybrids are still separate species as they occupy different habitats and are usually reproductively isolated. Phylogenic relationships Page 100 of 114 .plants ability for photosynthesis and so a compromise must be met between the two requirements. with similar evolutionary origins regardless of their functions in the adult of a species. Biological classification systems Can be either natural or artificial Artificial classification The grouping of an organism based on characteristics such as colour. etc Does not necessarily effect the evolutionary relationship of an organism. We classify things so that we can make sense of the world around us and to know where to find things we want. Section 14. size. What is a species? A group of organisms similar to each other with similar features that can interbreed to produce fertile offspring. the system must be universal – it has to be usable by biologist anywhere in the world.1 – Classification What is classification? Classification is the grouping together of things on the basis of features that they have in common.
Kingdom Is the largest taxon Contains the most organisms.Most classification in use today are natural and aim to reflect the Phylogenic relationships – that is. Phylogenic Trees The closer the branches. the historical/evolutionary relationships between organisms. the closer the evolutionary relationship Hierarchy system Uses Phylogenic relationship to group organisms Modern biology places each organism into a taxa: a series of groups arranged in a hierarchy. Protoctista 3. with the fewest features in common There are five kingdoms: 1. Phylogeny is putting organisms into groups that reflect their evolutionary history A Phylogenic tree shows the evolutionary relationship between organisms. The study of a classification is called taxonomy Taxa • • • • • • • Kingdom Phylum Class Order Family Genus Species They are listed in descending order of size – species is the most exclusive group containing the fewest organisms. Each group is called a taxon and contains organism sharing some basic features indication they have a common ancestry. Animalia 5. Prokaryotae 2. Plantae Page 101 of 114 . Fungi 4.
mushrooms. Kingdom Animalia • • • • Are multicellular Have eukaryotic cells with no cell walls Develop from blastocyst Most animals: Ingest food into their digestive system. May be sub divided in the future due to the diverse mix of organisms • • • • All protocistians have eukaryotic cells Some posses’ cells walls. and are motile. chlorophyll and can even photosynthesise Others have no cell walls and are motile. Kingdom Protoctista Very diverse. Usually feed on dead organic material but some feed on living hosts Example.Kingdom Prokaryotae • • • Cells lack true nuclei Cells have circular DNA Cells to not have membranes around organelles Example: bacteria Organisms in other kingdoms have cells with true nuclei. contains most organism that do not belong in other kingdoms. Kingdom Plantae • • • • All plants: Multicellular have eukaryotic cells with a cellulose cell wall Are photosynthetic Examples: mosses. Some are unicellular. yeast. Kingdom Fungi • • • • • • Common features Non-cellulose wall Are non photosynthetic Eukaryotic cells Secrete enzymes to digest organic materials outside their cells and absorb the products of digestion. ferns conifers Page 102 of 114 . chromosomes and membrane bound organelles. others consist of billions of cells.
laid down by international codes. Family Primate families include hominidue.Viruses are not included in the classification system as they are acellular Autotrophs – make their own food Hetrotrophs – Consume food Phylum The kingdom Animalia contains approx. Class Classes include mammalia. Page 103 of 114 . reptilia. The first word is the generic name. Genus Species that are very similar to each other are places in the same genus. Species Homosapiens are the only existing species of the genus “homo” Binomial system Is used to name organism based on the system devised by Swedish naturalist Carl Linnaeus Is a universal system. Order Mammalian order includes primates. and anphibia. never a matter of personal opinion. Latin or Greek names are used Each organism is given a name with two words. Both the species and the genus are used when naming the organisms. carnivores. 10 phyla. the name of the genus and begins with an uppercase letter. The second word is the name of the species and it begins with an uppercase letter. etc.
They can be identified because they are 50% labelled The hybrid stands are separated out and the temperature increase in stages At each temperature the degree to which the two strands are still linked together is measured If the two species are closely related they will share many complementary bases The more complementary bases. The mixture is then heated so that the strands separate. Page 104 of 114 . causing them to separate.1 . the hydrogen bonds between the complementary stands break. DNA between two species can be compared in the following manor: DNA is purified. The degree of similarity in the amino acid sequence is of the same protein in two species will therefore reflect how closely related they are. The mixture is cooled allowing strands with complementary bases to join Some of the double strands formed with contain one strand for each species.Genetic comparisons using DNA and proteins Comparing the DNA and proteins of different species it is possible for scientists to determine the evolutionary relationships between them Comparison of DNA base sequences When a species gives rise to another species through evolution. Species that are more closely related will show fewer differences in their DNA compared to species that are less closely related. DNA hybridisation • • • • • • • • • • • • • When DNA is heated. Section 15.The two words must be printed in italic when typed or underlined if handwritten to show that it is the biological name. the more h bonds The greater the number of h bonds the higher the temperature required for separation The closer the species are related. It is then mixed with the DNA of the other species. extracted and cut into short pieces DNA from one species is labelled with a fluorescent radioactive tracer. the two species will be very similar genetically. the higher the temperature needed Comparison of amino acid sequences in proteins The sequence of amino acids in proteins is determined by the DNA. When they cool they reform.
one partner fails to respond correctly. The actions of a male act as a stimulus to the female. Section 15. the female seeks out a male who she can mate with. The principle behind this methods is the fact that antibodies of one species will respond to specific antigens on proteins. the more likely it is at being successful. • Identify a mate that is capable of reproducing – both partners need to be sexually mature and fertile. It is therefore important that during this time. Why is courtship behaviour necessary? It is important to ensure that mating is successful and that the offspring have the maximum chance of survival. who if she is receptive will respond with another stimulus to the male to carry out a further action. It is therefore possible for animals to identify each other by observing their behaviour.2.ensures that mating only takes place within the same species so that offspring are fertile.Immunological comparisons of proteins The proteins of different species can be compared using immunological techniques. Courtship behaviour ensures this by enabling individuals to: • Recognise members of their own species . the sequence is ended. • Forms a pair bond – leads to successful mating and raising of offspring • Synchronise mating – takes place when there is a maximum chance of the sperm and egg meeting Females have cycles during which they are only fertile for brief periods of time. The longer the courtship ritual lasts. If at one point. Page 105 of 114 .Courtship behaviour The behaviour of members of the same species is more alike than members of different species.
bacterial DNA can also mutate Mutation – changes in the sequence of bases. separate from the main DNA. Mutation A change of a single nucleotide base pair is a point of mutation Different types of mutation Substitution – The replacement of one nucleotide Deletion – loss of a nucleotide Insertion – Extra nucleotide added Duplication – A certain portion a nucleotide sequence of a gene is replicated. Binary fission is also known as vertical gene transmission Changes to DNA Like the DNA of all cells.1 – Genetic variation in bacteria Bacterial DNA Double stranded Contains two types of DNA Most bacterial DNA is organised into large circular molecules (as appose to linear DNA molecules found in eukaryotic cells). Inversion – The reversal of nucleotide sequence in a gene. Factors that induce DNA replication are called mutagens. Bacterial reproduction Reproduce asexually through binary fission In theory all of springs should be clones.Section 16. Mutations generally happen during DNA replication. Changing the base sequence leads to a different amino acid being coded for and therefore a different polypeptide is produced. Some bacterial DNA is found as plasmids these are small circular molecules of DNA. but in reality there is still genetic variation which can lead to antibiotic resistance. Harmful mutation Page 106 of 114 .
even a rare event such as a beneficial mutation is likely to happen in a relatively short time. Conjunction Conjunction is another way in which bacteria increase their genetic variation. Page 107 of 114 . Although rare.Most are harmful Man mutation result in a change in the shape of a protein so that the protein cannot function. beneficial mutations are bound to happen sooner or later if there are a large number or organisms. Beneficial mutations Very occasionally a mutation occurs that changes the phenotype so that an organism has a better chance of surviving and reproducing. As there are so many bacteria and they reproduce so quickly. The alternative amino acid sequence may not affect the proteins shape/function. Silent neutral mutations Some mutations have no effect because the mutation has occurred in a non-coding part of DNA The mutation may also produce a different codon for the same amino acid. Mutations that affect large sections of a gene are often lethal.
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Conjugation is also known as horizontal gene transmission. The donor cell has replicated one of its plasmids The plasmid is altered by an enzyme to make it linear. The pilus retracts to draw the cells closer together. • Antibiotics often include: Page 109 of 114 . Section 16. only microorganisms produce antibiotics.2+16.3 – Antibiotics/Antibiotic use and resistance What is an Antibiotic? An antibiotic is a substance produced by a microorganism that is capable of destroying or inhibiting the growth of another organism. but may give the producer a selective advantage. The plasmids in each bacterial cell then replicate to become double stranded. Antibiotics are not affective against viruses Antibiotics are known as secondary metabolites as they are not essential for the growth or reproduction of the organism that is producing them. Once the plasmid has been transferred to the recipient cell. the ends of the plasmid rejoin and become circular again. Their connection via pilus is broken and each cell is free to conjugate with other cells. however the term is generally used to include a wide rage of chemicals that damage pathogens.The donor cell produces a thin production called a “pilus” that connects the two cells. Producing antibiotics According to the strict definitions.
Bacteria are not killed but cell division is halted. The cell wall becomes so weak that the bacteria burst when water enters the cell by osmosis. Disrupt cell wall synthesis 2. The antibiotics inhibit the synthesis of peptide links that bind molecules together. bacterial cells become turgid when in a watery environment with higher water potential than they have. Disrupt protein synthesis Disrupting cell wall synthesis Antibiotics weaken the cell wall and cause osmoticlysis. so DNA is not replicated. Disrupt protein synthesis These antibiotics either inhibit protein synthesis or promote the synthesis of abnormal proteins. which can stretch. but stop them from reproducing. How bacteria resist antibiotics Remember that bacterial DNA can mutate Page 110 of 114 . The bacterial cells cannot synthesis enzymes and structured proteins. Chemicals such as penicillin which is produced naturally but altered chemically to make them more affective Chemicals such as chloramphemiol originally produced naturally but now entirely synthesised. Types of antibiotics Antibiotics that kill bacteria are called bactericidal antibiotics. Cell walls are made up of long chains of peptidoglycan molecules and allow the bacteria to tolerate osmotic influx of water. Some antibiotics. animals and plants. The antibiotics bind to the bacterial ribosomes but do not affect eukaryotic ribosomes which are larger.• • • Chemicals produced by microorganism. do not kill the bacteria. so it prevents expansion of the cell and halts further entry of water. The cell contents push against the cell wall. These antibiotics only work on bacteria that are actively growing and have no effect on bacteria lying dormant in the body. Disrupt DNA replication 3. Disrupt DNA replication These antibiotics disrupt the synthesis of nucleic acids. known as bacteriostatic antibiotics. Just as in plant cells. How antibiotics work Antibiotics work by interfering with some metabolic functions of microorganisms. There are three main ways antibiotics can do this: 1.
A gene for antibiotic resistant gene is only beneficial where there the bacteria are exposed to the antibiotic. when the cell divides. If a plasmid has a mutation that gives the bacteria resistance to an antibiotic. This antibiotic resistant strain is a result of natural selection. leaving only the resistant bacteria. These bacteria will have an advantage over the other bacteria and will out-compete the normal variety. This new protein could be an enzyme that is able break down the antibiotic before it has a chance to kill the bacteria. Conjugation is the main reason why some bacteria have become resistant to antibiotics. hence there is a selection pressure that kills off the bacteria without the gene. Antibiotic resistant strains An antibiotic resistant strain is a whole population that has become resistant to an antibiotic. An antibiotic strain can be produced by natural selection if the population was exposed to several antibiotics. Antibiotics do not cause mutations Antibiotic resistance most often results from mutations in the plasmid DNA Passing on antibiotic resistance Bacteria can pass on resistance in two ways. Multiple resistance A bacterium can accumulate several plasmid DNA via conjugation that give it resistance. until an antibiotic resistant strain has been produced. A plasmid conferring antibiotic resistance can pass from one bacterium to another Conjugation can also occur between members of the same species. each daughter cell will receive the gene for resistance. the greater the chance that mutant bacteria will have multiple resistance.A mutation is the change of base sequence in a gene that results in a new protein produced. Natural selection The frequency of the allele for resistance consequently increases and will continue to increase with each succeeding generation of the bacteria. Overuse of antibiotics may have provided an increased selection pressure in favour of resistant bacteria. Conjugation – Bacteria can also pass and receive plasmids from other bacteria in the process called conjugation. The greater the amount of antibiotics used. MRSA Page 111 of 114 . binary fission/conjugation Binary fission – Both the plasmid and the larger circular DNA in the bacterium replicate prior to cell division.
Resistant to several antibiotics Because of its multiple resistances. MRSA is found on many individuals skin without any ill effects but these people could pass the bacterium to somebody else via skin to skin contact. • • • Species diversity – refers to the number of different species and the number of individuals of each species within anyone community Genetic diversity – refers to the variety of genes possessed by the individuals that make up any one species Ecosystem diversity – refers to the rand of different habitats within a particular area Species diversity has two components: 1. Absolute cleanliness is particularly important in wards where patients may have open wounds. infections caused by this bacterium are difficult to treat. The number of different species in a given area 2.1 – Species diversity Biodiversity is the term given to describe the variety in the living world. The proportion of the community that is made up of an individual species Species diversity is calculated using the index that follows: d=N(N-1) Page 112 of 114 . so the focus is on preventing transmission. Anyone in contact with patients is required to wash their hands. If the bacterium gets inside the body it can cause deadly infections. Section 17.
This means that smaller areas are only available for other species. Page 113 of 114 . Agriculture and deforestation are two examples of processes carried out by humans that have the affect of lower biodiversity.2 – Species diversity and Human activities The impact of providing more food at a lower cost as resulted in less biodiversity. Impact of agriculture • • • • Ecosystems develop overtime. therefore these areas must be occupied by the species the farmer feels is most desirable. Selective breeding has reduced the genetic variation of certain species and therefore reduced the variety of alleles in the population.Σn(n-1) Where: • • • • d = species diversity index N = total number of organisms of all species n = total number of organisms of each species Σ = the sum of Number (n) found in habitat X 10 10 10 10 10 Σn(n-1) N(n-1) 10(9)=90 10(9)=90 10(9)=90 10(9)=90 10(9)=90 450 Number (n) found in habitat Y 3 5 2 36 4 Σn(n-1) N(n-1) 3(2)=6 5(4)=20 2(1)=2 36(25)=1260 4(3)=12 1300 Species A B C D E Habitat X: d = 50(49) 450 =5. Any particular area can only support a certain amount of biomass.88 Habitat X has a higher value for d and therefore has more species diversity Section 17. this therefore means that they have increased in species diversity. becoming more complex communities with many individuals of many different species.44 Habitat Y: d =50(49) 1300 =1.
• • Species will compete for the resources around and many will not survive. Species diversity is reduced due to deforestation because: • Habitats are destroyed • Species are not adapted to live anywhere else • The organism in the species will not survive as a result • This note only reduces the amount of organisms in a species. The most serious consequence of deforestation is the loss of bio diversity where many species are killed each year as a result. Impact of deforestation Forests provide numerous habitats where species have become adapted to live. The overall affect is that the species diversity is lowered. but also reduces the number of different species. Page 114 of 114 . • The overall affect is a reduction in the species diversity for the given area. Deforestation is the permanent clearing of forests and the conservation of the land to other uses such as agriculture. In addition pesticides are used to kill off these species as they can affect crop growth.