Muhammad Nazar & Wael At-Thubaity Department of Chemistry School of Science and Technology UNE-University of New Engand Armidale

- NSW Australia

Determination of Ca, Na and Mg in Milk

Calcium
 Calcium is essential for bone development

 Food contain calcium including soybean, sesame

seed, tofu, cheese, milk and many more  Dairy Milk contain 120 to 200 mg Calcium per 100 mL

Calcium Requirements/day
Age 0–6 months* 7–12 months* 1–3 years 4–8 years 9–13 years 14–18 years 19–50 years 51–70 years 71+ years Male 200 mg 260 mg 700 mg 1,000 mg 1,300 mg 1,300 mg 1,000 mg 1,000 mg 1,200 mg Female 200 mg 260 mg 700 mg 1,000 mg 1,300 mg 1,300 mg 1,000 mg 1,200 mg 1,200 mg 1,300 mg 1,000 mg 1,300 mg 1,000 mg Pregnant Lactating

http://www.osteoporosis.ca/index.php/ci_ id/5535/la_id/1.htm

Sodium

Sodium
 Sodium ion is electrolyte that is required in blood

regulation.  But over consumption of sodium lead to stomach cancer and high blood pressure.  Sodium mainly come from table salt  Food contain sodium including fruits (small amount), milk, meat and etc.

Magnesium
 helps maintain normal muscle and nerve function,

keeps heart rhythm steady, supports a healthy immune system, and keeps bones strong. Magnesium also helps regulate blood sugar levels, promotes normal blood pressure, and is known to be involved in energy metabolism and protein synthesis  Nuts, bean, milk, spinach are known as good sources of Mg

Samples
 Samples were collected from Woolworth

 Milk from 5 different brands were examined in this

experiment including Norco, Liddales, Woolworths, Nutrisoy and Devindales.

METHOD
 To test Ca and Mg we used EDTA complex titration

involving Erio Black T indicator.  To determine Ca only, we employed back titration at high pH with hydroxy naphtol blue indicator  Sodium content was analysed using Flame Spectrophotometer.

Sodium content
Calibration Curve
60 y = 1.35x + 8.8333 R² = 0.9716

50

40

Reading

30

Series1 Linear (Series1)

20

10

0 0 5 10 15 20 Concentration (ppm) 25 30 35

Sodium in Sample compared to label
Sodium
Experiment Label

98.2 89.78 91.47 91.47

101.57

58 43 43

40

40

Norco

Liddells

Woolworths

Vitasoy

Devondale

Calcium in 100 mL sample
Calcium
Exp Label

222

200 160 133.6 120 131

130 123

119 120

Norco

Liddells

Woolworths

Vitasoy

Devondale

Magnesium/100 mL milk

Norco Liddale

Woolworths

Vitasoy

Devondale

Exp 58.8 55.8
Label NA NA

53.6
NA

56.5
50

64
NA

Conclusion
 Calcium and Magnesium in milk can be determined

by using titration technique involving two different indicators Erio Black T and hydroxy phenol.  The amount of Ca2+ determined in this experiment relatively agree with given labels  The amount of sodium found in this experiment much greater than that given on the labels due to the interference of organic molecules like protein and carbohydrate present in samples