Multiple Extraction of Caffeine from Dried Tea Leaves Using Dichloromethane

Abstract The experiment was intended to find how much caffeine is present in a 10 g black tea leaves sample and to test the efficiency of multiple extraction. The caffeine extracted from the tea leaves was 1.7 grams which compromises 17 % of the 10 g tea leaves. The tea leaves have undergone series of procedure to extract its caffeine. It was boiled for 10 minutes in a mixture containing 4.4 g dissolved anhydrous sodium carbonate in 100 ml of distilled water. The aqueous solution was then extracted using liquid-liquid extraction with the aid of 30 ml of dichloromethane twice in a separatory funnel. In an Erlenmeyer flask half a spatula of anhydrous sodium sulfate was added to the organic layer. It was then decanted onto an evaporating dish and was evaporated to dryness.

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Introduction Extraction is the process of obtaining something from a mixture or compound by chemical or physical or mechanical means. There are three basic types of extraction: liquid/liquid, acid/base and solid/liquid [1]. The liquid-liquid extraction is the method that is useful in the extraction of caffeine from tea leaves so, we’ll give it a further discussion. Liquid–liquid extraction, also known as solvent extraction and partitioning, is a

method that utilizes the different solubility of compounds to be separated in two immiscible solvent commonly, water and organic substance. It is an extraction of a substance from one liquid phase into another liquid phase [2]. This type of extraction can be further divided into two namely simple and multiple extractions. The objectives of the group in this experiment are the following :(1) Extract caffeine from dried tea leaves (2) calculate the percentage yield of caffeine for both extraction procedures.

Methodology A. Preparation of the Aqueous Layer ( anhydrous sodium carbonate solution) In a small Erlenmeyer flask, 4.4 g of anhydrous sodium carbonate was placed and 100 ml of distilled water was added. The

mixture was then heated in a water bath until the solid has dissolved. B. Brewing of Tea Leaves with the Aqueous Layer After the preparation the mixture, of tea

of 10 g

C. Fig. The mixture was decanted onto a tared evaporating dish and was evaporated to dryness.html D. The weight of residue was then calculated by getting the di ffe ren ce bet w een t he m ass o f t he evaporating dish containing the residue and the mass of the evaporating dish before the presence of residue on it.4 g of anhydrous sodium carbonate + 100 ml distilled water Hot bath . The separatory funnel was then swirled 15 times in a set of 8 (Every after 15 times of swirling let the gas inside to get out by setting the stopcock to its opened position. The teabag was removed and squeezed by pressing it against the side of the flask using a glass rod to collect the remaining liquid. The anhydrous sodium sulfate will remove the water byabsorbing the water. boil for 10 minutes .wellesley. The flask was covered and the tea mixture was allowed to boil for 10 minutes (you should start the timer when the formation of the first set of bubbles has appeared) on a hot plate. It is also important to know that you should secure the stopper with your other hand when performing the swirling to prevent abrupt outflow of the liquid from the separatory funnel due to the pressure building up inside.edu/Chemistry/chem211la b/Orgo_Lab_Manual/Appendix/Techniques/Extr action/extraction_n. The organic layer was drained into a clean Erlenmeyer flask. Performed it was left to stand until the separation between the two layers was clearly visible. Collecting the Residue (caffeine) from the Organic Layer Extract The organic layer extract was transferred into an Erlenmeyer flask containing half a spatula of anhydrous sodium sulfate. 1 Set-up of liquid-liquid extraction Retrieved from: http://www. The organic layer extracts 4. The steps in the extraction were repeated for twice with the same amount of dichloromethane. Liquid-liquid extraction with dichloromethane The aqueous solution was mixed with 30 ml of dichloromethane in a separatory funnel.)After the swirling was were all combined and the aqueous layer w a s d i s c a r d e d .leaves contained in a teabag was added.+ 10 g dried tea leaves. this will prevent the buildup of pressure inside.

Results obtained from the experiment X1= amt.23 g of caffeine was left in the sample . 122.90 grams 1.6 grams – 122. of extract left in the sample X0= amount of sample Vaq= Vol. of organic layer n= no. of extraction ( ) ( ) X1= 0. evaporate to dryness Residue (caffeine) Fig. decant. swirl.90 grams = 1. of Aqueous layer Calculations: Weight of Residue: 124. 2 Schematic Diagram of Multiple Extraction Results and Discussions ( ) Weight of the tea leaves Weight of the evaporating dish and residue Weight of evaporating dish Weight of the extracted cafeine Percentage yield of caffeine 10 grams 124.7 grams caffeine Vorg = Vol.7 grams ( 17 % ( ) ) Table 1.60 grams ( ) The percent yield is 17%. drain Discarded tea leaves Organic layer Aqueous layer + ½ spatula of anhydrous sodium sulfate.Aqueos solution +30 ml dichloromethane (in each extraction).

C.(7/22/2012).web. Liquid-liquid Extraction. The percentage yield was calculated by getting References [1]Kilway.the ratio between the mass of the extracted caffeine and the mass the dried tea leaves multiplied to 100.umkc. which compromises 17 % of the 10 gram sample of dried tea leaves.K.edu/drewa/Chem321L/Handouts/Lab3Extraction1FS2010.pdf [2] Heden.7 grams. (7/22/2012). Retrived from http://d. Retrieved . After the entire procedure the extracted caffeine from the tea leaves was found to be 1.Extraction.

ttu.depts. pdf .fromhttp://www.edu/che/classes/che4232/notes/5%20Liquid%20Liquid%20Extraction.

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