PRELIMINARY PRODUCT INFORMATION

Control Number: 024-0005-14 Revision: B

ADVIA 120 Hydraulic Troubleshooting Guide
Date of Issue: Subject: Products: 3/24/99 Hydraulic Troubleshooting Guide ADVIA 120

ADVIA 120 HYDRAULIC TROUBLESHOOTING GUIDE

The following is a troubleshooting chart that attempts to guide Field Service through some common hydraulic problems on the ADVIA 120. This is a REVISED version which contains extensive changes to page 14 concerning troubleshooting probe clogs and conductivity problems. Important Tips: When using this chart, the obvious has been eliminated. It assumes that on board Hydraulic Functions like System Wash and Flowcell Washes have been performed. Remember to return devices and tubing to their original positions before proceeding to the next procedure. If using the exerciser and opening Valves, do a Valve Check to close all valves.

PPI #024-0005-14 Hydraulic Troubleshooting Guide March 1999 Rev B

.........................................................................................................................................................................................................................................................................................PRELIMINARY PRODUCT INFORMATION Control Number: 024-0005-14 Revision: A Table of Contents TROUBLESHOOTING: RBC PATHWAYS .......................................................................... TROUBLESHOOTING: IMPRECISION .........................................................SINGLE METHOD..................................................................................................................................................................................................................................................................................................................................... TROUBLESHOOTING: PEROX PATHWAYS............................................................................................................................................................................... TROUBLESHOOTING: LEAKS at ASPIRATION MODES............................................................................................................................... Page 3 ................... TROUBLESHOOTING ..........DOME VALVES........................................................... TROUBLESHOOTING: CONDUCTIVITY PROBLEMS (PROBE CLOGS) ....... CONTINUOUS IMPROVEMENT IDEAS .......................................................................................................................................... TROUBLESHOOTING: CLOGS at ASPIRATION MODES................................................................................................... REAGENT DIAPHRAGM.................................................................................................................................................................................................

4. 2. Open V20 and measure time to empty the chamber. 7. 3. 5. do an F/C Wash. and V13. Open V21. UFC Vacuum should not differ from Main vacuum by more than 5 in Hg. If RBC shuttle pathway passes. Repeat test. 1.RBC Shuttle and Flowcell 1. Page 4 . Go to Exerciser. 3.CFM. syringe. If test fails again. V20. V9. Close Valves. 6. Monitor for at least 1 min. V10. Go to Exerciser. If RBC Shuttle Pathway Passes. Note: Capacity of Vacuum Pump should be 24"-25" Hg. and UFC Vacuum. If UFC Vacuum Test Passes. Open V9. 8. Repeat this test. Fill a 10 ml syringe with 50% bleach and attach to RBC/Baso sample line going into the UFC. Access the Pneumatics Screen and verify UFC and Main Vacuum readings. Check fittings on waste jug including spigot and waste exit port. V10 and allow vacuum to pull bleach through pathway. perform RBC Shuttle pathway test to isolate. bypass the in-line check valve in the waste line to the waste jug. Dispense RBC reagent into reaction chamber by putting Shear Valve in Dispense position and actuate V80. V21 and allow vacuum to pull bleach through pathway. Repeat this test.RBC Shuttle Pathway 1. 4. 1. Measures pathways of V9. V21 and UFC Vacuum.PRELIMINARY PRODUCT INFORMATION Control Number: 024-0005-14 Revision: A TROUBLESHOOTING: RBC PATHWAYS COMPLAINT PATHWAY TESTED STEPS TYPICAL TIME RESULT CORRECTIVE ACTION [If Pathway Tested Fails] Rate flags in RBC/Baso/Reti c Channels No Results 1. Return to Pneumatics Screen and monitor UFC and Main Vacuum. 2. 2. Go to Exerciser. RBC syringe . Close all valves and allow vacuum to recover. Dispense RBC reagent into reaction chamber by putting Shear Valve in Dispense Position and actuating V80. Restart test. this could cause vacuum problems. Open V20. V21. 3. Clean out the waste manifold. 3. 1. If pump is compromised. 4. < 8 sec. 6. and system has an Auto-waste configuration. Close Valves. go to RBC Shuttle Pathway Test. Open V20 and measure time to empty the <11 sec. check for clogs in the waste lines from the UFC and in the lines to and from the waste manifold (behind power supply drawer). 2. Go to Shuttle Chamber drain if unable to complete. If test fails again. 4. Close all valves and allow vacuum to recover. Fill a 10 ml syringe with 50% bleach and attach to the side of the sample syringe. 4. clean or replace the CFM. Measures pathways of V9. 3. Open V21 and V9. Disconnect RBC flowcell sample line at top of sample syringe Disconnect RBC flowcell shuttle line at the bottom of flowcell and reconnect to nipple at the top of the sample syringe. 5. 4. V7. Open V5. 2. 2. Restart test. V20. 3.UFC Vacuum Test 1. 2. If it still fails. 3. Replace large vacushield on the waste jug.

5.) Page 5 . Open V21. Go to Exerciser. let bleach soak with valves open and compressor on for up to 1 hour. 4. Measures pathways of V22. 2. 3. 2. V21 and UFC Vacuum. 4. (Opening and closing V21 'pulsating' intermittently may also help.RBC Shuttle Chamber Drain (V21) 1. 5. Connect 8" length of 0. If necessary. Submerge tubing in 10 ml of RBC Sheath/Rinse.081´tubing to RBC Vacuum Shuttle chamber Vent. Attach syringe with 50% bleach to flowcell shuttle line going to the UFC. Open V21 and gently push bleach into RBC shuttle Chamber. Open V20 and V22. Open V22 and measure the time to aspirate 10ml.PRELIMINARY PRODUCT INFORMATION Control Number: 024-0005-14 Revision: A chamber. 3. 4. <10 sec. Verify tubing is connected to shuttle vent and placed in overflow container. 1.

4. 3. Do flowcell wash Verify connections at DP5. 4. Open V23.RBC Sheath Dispense 1. Verify tubing going to waste manifold behind the power supply drawer is not constricted. If test does not pass. 3. the check valve inside the straw is the problem. 5. <35 sec. Measures pathways of V23. (This is the tubing from V23 to sheath syringe. Verify Waste Manifold does not have clog in it. Disconnect sheath input to RBC sheath syringe. Sheath filter. and Waste jug. Verify 5psi and 20” hg at DP5. Go to Exerciser. Waste Manifold. Page 6 . Attach a syringe filled with 10 ml of Rinse/Sheath to the place you just removed the fitting at V23. 5. 6. Remove the fitting going into V23. 2. If the test passes. V27. Repeat this test. Verify tubing from waste manifold to waste jug is not constricted. Flowcell. 4. Repeat this test. 5. Open V27 and measure the time to aspirate 10 ml. If the test passes. Go to Exerciser. disconnect the tubing to the Sheath/Rinse straw and insert tubing directly into Sheath/Rinse container. 2. Verify there is no leakage of sheath filter and it is < 1 month old. Remove Sheath/Rinse straw and remove the filter. 6. 2. [Note: you will have to place straw into container to overcome the level sensor.RBC/Baso Sheath Syringe to Waste 1.) Actuate V34 to pressurize the RBC sheath diaphragm pump. 4. Reinstall straw. V19. Measures pathways between Universal Sheath/Rinse. and V23 1. < 14 sec. 3. the filter is the problem. Verify DP5 and check valves are working properly. 3. Open V19 and measure dispense time. DP5 (V34). Prime Sheath/Rinse 5 times. 2. Change filter if suspect. 7. Open V23. Verify V23 and V37 are opening.PRELIMINARY PRODUCT INFORMATION Control Number: 024-0005-14 Revision: A TROUBLESHOOTING: RBC PATHWAYS continued COMPLAINT PATHWAY TESTED STEPS TYPICA L TIME RESULT CORRECTIVE ACTION [If Pathway Tested Fails] 5. 1.

Check fittings on waste jug including spigot and waste exit port. Return to Pneumatics Screen and monitor UFC and Main Vacuum. V7. 8. this could cause vacuum problems. Open V16 and measure time to empty the chamber. 3. Unscrew fitting from side of Perox sample syringe Rate flags in Perox Channel Perox Carryover 1. 2. 4. 3. <1min. 4. 4. Repeat test. remove chamber and clean drain port according to Cleaning Perox Chamber Ports (ADVH23). check for clogs in the waste lines from the UFC and in the lines to and from the waste manifold (behind power supply drawer). Restart test. V6. 6. Fill Perox chamber with CN-Free Hgb Rgt.Perox Shuttle Pathway 1. and V13. Access the Pneumatics Screen and verify UFC and Main Vacuum readings. 2. 2. Go into Exerciser. 3. 2. 5. 7. Restart test. Manually fill Perox Chamber to very top with Sheath/Rinse or DI Water. do a flowcell wash. do a Perox Shuttle Pathway Test. Measures pathways between Perox Reaction chamber. V5. Clean out the waste manifold. 5. V15. 4. If test fails again. Also UFC vacuum. If necessary. 2. 3. 2. Close all valves and allow vacuum to recover. 1. Replace large vacushield on the waste jug. Manually fill Perox Chamber to very top with Sheath/Rinse or DI Water. Go to Exerciser. 3. 2. 2. If test fails again. 1. <10 sec. bypass the in-line check valve in the waste line to the waste jug. Open V5 and V7.UFC Vacuum Test STEPS TYPICA L TIME Monitor for at least 1 min. 6. Fill Perox chamber with CN-Free Hgb Rgt. Go to Exerciser. V10. do a Perox Shuttle Chamber Test. Close all valves and allow vacuum to recover. Open Valves 17. and system has an Auto-waste configuration. 4. < 3 sec. CFM and V16 (shuttle chamber). 2. If pump is compromised. Go to Exerciser. UFC Vacuum should not differ from Main vacuum by more than 5 in Hg. clean. Measures pathways between Perox Sheath. Go to Exerciser. Page 7 .Perox Chamber Drain 1. Open Valves 5. RESULT CORRECTIVE ACTION [If Pathway Tested Fails] 1. 3.PRELIMINARY PRODUCT INFORMATION Control Number: 024-0005-14 Revision: A TROUBLESHOOTING: PEROX PATHWAYS COMPLAINT PATHWAY TESTED 1. 3. If Perox Chamber Drain Test Passes. Open Valve 7 and measure time to empty chamber. If necessary. replace CFM. 5. If Perox Shuttle Chamber Test passes. 3. If Perox Shuttle Pathway Test Passes. Open V5. Note: Capacity of Vacuum Pump should be 24"-25" Hg. flowcell 1. Measures pathway between Perox Reaction chamber and V7. Manually fill Perox Chamber to very top with Sheath/Rinse or DI Water. Repeat twice. 4. DP1(V26). If UFC Vacuum Test Passes: Perform Perox Chamber Drain Test to isolate.Perox Shuttle and Flowcell 1. 4. Sheath Filter.

Page 8 . Disconnect Perox flowcell sample line at top of sample syringe. 6. Open Valves 17. 6. 4.PRELIMINARY PRODUCT INFORMATION Control Number: 024-0005-14 Revision: A 3. Repeat test. Open V16 and measure time to empty chamber. 5. 5. Flush final time and repeat with water. Open Valves 5. 6. and V24. 4. and attach to flowcell cleaning syringe. Repeat flushing 2 times. 5. Disconnect Perox shuttle line at the bottom of flowcell and reconnect to nipple at the top of the sample syringe. Push and pull syringe to flush CN-Free back and forth. Open V7 to drain. allowing reagent to sit 10 minutes.and 6.

Perox Sheath Dispense 1. 5. Verify there is no leakage of sheath filter and it is < 1 month old. 4. 6. 2. Verify connections at DP1. 2. Unscrew Perox sample tubing from side of block and attach to flowcell cleaning syringe filled with CNFree Hgb Rgt. Go to Exerciser. 4. <1 min. 2.Perox Sheath Syringe to Waste 1. 5. Measures pathways between Universal Sheath/Rinse. Attach a syringe filled with 10 ml of Rinse/Sheath to the place you just removed the fitting at V24. Actuate V26 to pressurize the Perox sheath diaphragm pump. 4. Connect 8 in. Go to Exerciser. Flush final time and repeat with water. Repeat flushing 2 times. <17 sec Measures pathways of V24. Verify DP1 and check valves are working properly. Open V24. 2. 3. Flush CN-Free back and forth. 4. 1. Go into Exerciser. 7. 5. DP1 (V26). 5. Verify that reagent filter to Perox Reagent bottle is not clogged. Do flowcell wash. 1. 1. 3. 4. Open V24. Open V17 to drain. Verify tubing going to waste manifold behind the power supply drawer is not constricted.PRELIMINARY PRODUCT INFORMATION Control Number: 024-0005-14 Revision: A TROUBLESHOOTING: PEROX PATHWAYS continued COMPLAINT PATHWAY TESTED 5. Place into small container. length of 0. Waste Manifold. 3.081” tubing to Perox Vacuum Shuttle Chamber VENT. Measures pathway from Perox shuttle to V17. Verify 5psi and 20” hg at DP1.Perox Shuttle Chamber Drain STEPS TYPICAL TIME <1 min. allowing reagent to sit for 10 minutes. Go to Exerciser. Open V17 Open V18 and measure time to aspirate 10 ml. Open V25 and measure the time to aspirate 10 ml. Also UFC vacuum. Sheath fllter. 4. Verify V24 and V25 are opening. Page 9 . Verify Waste Manifold does not have clog in it. Open V16 and V18. Disconnect sheath input to Perox sheath syringe. 2. 2. 3. RESULT CORRECTIVE ACTION [If Pathway Tested Fails] 1. Open V15 and measure dispense time. V25. Verify tubing from waste manifold to waste jug is not constricted. Remove the fitting going into V24. Submerge tubing into 10 ml RBC Sheath/Rinse or DI Water. 3. V15 Flowcell and V24. 3. and Waste jug.

close valve and allow bleach is sit to try to deteriorate clog. 7. Submerge open end of tubing in 10 ml of RBC Sheath/Rinse. tubing from 1. 2. Verify UFC Vacuum with UFC Vacuum Test. Go to Exerciser Disconnect tubing leading to V44 at the Autosampler Centering Collar. Go to Exerciser Disconnect tubing leading to V45 at the Autosampler Centering Collar. close valve and allow bleach is sit to try to deteriorate clog. Autosampler Rinse Vacuum Lines . 3. Verify nipple at wash block is not clogged. V4 and try to dispense 50% bleach into valve area with alternate slugs of air using transfer pipette. 1. 3. replace coupling and tubing and aspirate 50% bleach through specific valve. 7. Leaks from Manual Open Tube Probe Manual Open Tube Sampler Vacuum Line at Wash Block 1. Measures UFC Vacuum. 2. Try to aspirate 50% bleach into tubing and alternate with slugs of air. <17 sec. While bleach is being aspirated. Measures UFC Vacuum. 2. Go to Exerciser. 6. <23 sec.] If one fails: verify tubing to specific valve is not clogged or pinched. 1. 2. Remove coupling and verify that tubing connection at coupling is secure and that connector has no clog. Verify tubing to V46 is not clogged or pinched. Clean out ports and proceed to procedures below for checking waste lines. Leaks from Manual Closed Manual Closed Tube Sampler 1. If both tests fail. Verify tubing to V4 is not clogged or pinched. 2. 5. 8. 3. Open V4 and measure aspiration time. Aspirate 50% bleach into tubing and alternate with slugs of air. 2b. 4.V44 1. While bleach is being aspirated. 6. pathway at UFC for V44. 4. <26 sec.. Checks for clogs in centering collar. Remove sample probe. Measures UFC Vacuum. <36 sec. Measures UFC Vacuum. Repeat test. Submerge open end of tubing in 10 ml of RBC Sheath/Rinse. Disconnect tubing leading to V4 from Wash Block. Also. 2. verify there is no clog at inlet of UFC. With coupling removed. 4. tubing from Wash Block to V4. tubing to AS centering collar. If not successful. verify there is no clog at inlet of UFC. open specific valve and try to dispense 50% bleach into valve area with alternate slugs of air.V45 1. Remove Wash Block. If not successful. 5. Centering Collar 2a. Open V45 and measure aspiration time. Repeat test. 2. Open V44 and measure apiration time. Remove centering collar and check for clogs at centering collar nipples. tubing to AS centering collar. pathway at UFC for V45. Remove coupling and verify that tubing connection at coupling is secure and that connector has no clog. 1. Submerge open end of tubing in 10 ml of RBC Sheath/Rinse. 4. 1. verify UFC Vacuum with UFC Vacuum Test. pathway at UFC for V4. Verify UFC Vacuum with UFC Vacuum Test. replace coupling and tubing and aspirate 50% bleach through specific valve. Go to Exerciser.PRELIMINARY PRODUCT INFORMATION Control Number: 024-0005-14 Revision: A TROUBLESHOOTING: LEAKS at ASPIRATION MODES COMPLAINT PATHWAY TESTED STEPS TYPICAL TIME RESULT CORRECTIVE ACTION [If Pathway Tested Fails] Leaks from Autosampler Needle/ Centering Collar 1. Autosampler Rinse Vacuum Lines . Also. 6. 3. [Note: both V44 and V45 lines could be clogged or pinched. Page 10 . 5. With coupling removed. Remove MCTS Needle Chamber. 4. 3..

replace coupling and tubing and aspirate 50% bleach through specific valve. Aspirate 50% bleach into tubing and alternate with slugs of air. the Needle Chamber to V46. open V46 and try to dispense 50% bleach into valve area with alternate slugs of air. Check side nipple on MCTS chamber is not clogged or tubing pinched. 6. 7.PRELIMINARY PRODUCT INFORMATION Control Number: 024-0005-14 Revision: A Tube Needle Vacuum Line at Needle Chamber 3. 5. 8. With coupling removed. Submerge open end of line in 10 ml of RBC Sheath/Rinse. While bleach is being aspirated. pathway at UFC for V46. close valve and allow bleach is sit to try to deteriorate clog. 5. Remove coupling and verify that tubing connection at coupling is secure and that connector has no clog. 4. Open V46 and measure aspiration time. Repeat test. 4. Page 11 . Disconnect line leading to V46 on side port of Needle Chamber. 3. If not successful.

Verify that rinse is not seeping past valve into the chamber. 2. replace the pilot valve. Submerge open tube probe in 10 ml of RBC Sheath/Rinse. V73. use sample probe and place selector valve in open mode. clean shear face off line and clean ports on rear shear face. Autosampler Verify UFC Vacuum with UFC Vacuum Test. V37. If testing AS aspiration. 2. 2. pathway from specific aspiration mode through shear face. 3. Go to Exerciser. Verify that V1 and V47 are closed. Access the Pneumatics Screen and verify UFC and Main Vacuum readings. 4. Monitor for at least 1 min. Look for constrictions or leaks. Open V72.PRELIMINARY PRODUCT INFORMATION Control Number: 024-0005-14 Revision: A TROUBLESHOOTING: CLOGS at ASPIRATION MODES COMPLAINT PATHWAY TESTED STEPS TYPICA L TIME RESULT CORRECTIVE ACTION [If Pathway Tested Fails] Poor Aspiration from Any or All Modes Poor Aspiration in certain Selectivity Probe Clogs Failed Aspiration Aspiration Modes: Open MCTS 1. Checks for sealing of the dome valves 1. Pressurize both rinse diaphragm pumps (V28 and V29) Observe suspect rinse valves (V2. Page 12 . 1. 6. and V74 for CBC/Diff/Retic 4. 2. Probe Clogs Failed Aspiration Rinse Dome Valve Sealing Test Poor Aspiration or Dripping Probe UFC Vacuum Test 1. V11. V8. 2. use tubing attached to the bottom of the needle and place selector valve in MCTS. use tubing attached to the bottom of the needle and place selector valve in AS. Measures UFC Vacuum. Open V1 and measure time to aspirate 10 ml. By pass the selector valve by using syringe coupling to connect the sample line from the UFC directly into the fitting containing the clot filter on the selector valve. V66). Check the clot filter. Follow this with DI water.] If bypassing the selector valve does not correct.e. as a last resort. If testing MCTS aspiration. Open V47 3. Go to Exerciser. 1. 3. Check small vacushield filter for integrity. UFC Vacuum should not differ from Main vacuum by more than 5 in Hg. Open V72 for CBC. If testing open mode aspiration. Repeat procedure for test using 50% bleach and alternating with slugs of air. 4. 1. If there is one valve in particular that is leaking (i. Selectivity: CBC CBC/Diff CBC/Diff/Reti c 1. Go to Exerciser Adjust 40 psi down to 30 psi. 3. Check fittings on waste jug including spigot and waste exit port. <1min 50 sec. 4. Check pilot pressure (40 psi) path from the pneumatic drawer to the UFC. rinse entering chamber during test) and it is not a dome valve failure. Replace large vacushield on the waste jug. [If significantly faster. Repeat test. selector valve. troubleshoot selector valve for clogs or alignments. 2. 5. 3. V73 for CBC/Diff Open V72. and all associated tubing in pathway. Monitor for at least 1 min. clot filter. 5.

2.PRELIMINARY PRODUCT INFORMATION Control Number: 024-0005-14 Revision: A Compressor Timeout Vacuum Out of Range 3. Close all valves and allow vacuum to recover. Restart test. 6. V10. If test fails again. V7. Open V5. Page 13 . this could cause vacuum problems. 8. Clean out the waste manifold. 5. 4. If pump is compromised. 7. Return to Pneumatics Screen and monitor UFC and Main Vacuum. 3. Restart test. If test fails again. bypass the in-line check valve in the waste line to the waste jug. Note: Capacity of Vacuum Pump should be 24"-25" Hg. and V13. check for clogs in the waste lines from the UFC and in the lines to and from the waste manifold (behind power supply drawer). and system has an Auto-waste configuration. Repeat test. 4. Close all valves and allow vacuum to recover.

RESULT CORRECTIVE ACTION [If Pathway Tested Fails] 1. An alternate method to test conductance is to use the exerciser. Clean out sample pathway across Page 14 . Analog test points Cond#1 = TP 9-4 &TP11-1gnd or Determine if voltage is because Offset >0. > 20 SEC. During the next aspiration. N/A 4.0V is measured at the analog test point when the path should be dry. Go into syringes pumps section and put the selector valve in the open position. If not the parallel node board is suspect. Consult TTN before changing UFC. or needle does not retract. Determine which detector is not functioning properly by aspirating sample and watching green light and blood.47. If voltage is >0. Do UFC Vacuum test. Check for probe clogs in all aspirate modes.6v.6v is set at threshold TP’s. The conductors should be detecting when the sample is present and not conducting when the line is dry. Thus you can aspirate while looking at the indicators screen in the exerciser. If light continues flashing. Mode 2 . 3. <20 sec. You can now aspirate continuously from the open tube mode. Remove cable from UFC to Scrambler brd. 3.6. Check for leaks in the waste system. 4.5V to -5. Mode 1B Make sure threshold of 0. is v due to cable cross talk or grounding. Make sure pins in cable connectors are making good contact. 4. Check for Aspiration time <20 sec. Mode 1 . Clog only in one mode. Re-tighten screws going to the conductivity sensor on the back of the UFC. read voltage of effected detector on Parallel Node brd. Mode 1A A voltage of-0. Mode 1B A voltage of less than -0. Mode 1A 2. Go to CLOGS at ASPIRATION MODE Go to Step 4.5v is measured at the analog test point when the path is dry but the system still reports conductance. Go to CLOGS at ASPIRATION MODE Probe Clogs 1.3 but goes back up when reconnected rinse may be left in pathway(usually this voltage will decrease as rinse “dries”).PRELIMINARY PRODUCT INFORMATION Control Number: 024-0005-14 Revision: A TROUBLESHOOTING: CONDUCTIVITY PROBLEMS (PROBE CLOGS) COMPLAINT PATHWAY TESTED Aspiration Mode STEPS TYPICA L TIME < 20 sec. In the pneumatics section move the shear valve to the aspirate mode. If voltage <0. There are two failure modes. #1 not detecting change in state. 1. If passes.A sensor is not conducting when the aspiration path has sample in it. Mode 2A Check cable continuity. Note serial # and mfg of UFC.72. Check version of parallel node bd. 2. Check for signal transition at TP9-3 or TP11-3.A sensor is conducting when the aspiration path should be dry. If blood is aspirated straight to waste. Use a high control for worst case. Cond. This is the digital signal(TTL) out for conductance. #2 not detecting change. do Rinse Dome Valve Sealing test. Clean out sample pathway with bleach solution.at the test points listed below before the sample reaches detectors(dry) and when the sample contacts the sensors(wet). Go into the valves section and open valves 1. Cond.3. 3. It should toggle when the analog signal at TP9-4 or TP11-4 passes through –0. Mode 2A A voltage of less than – 1v is measured at the analog test point when the path has sample in it. 2.

Monitor for at least 1 min. If there is one valve in particular that is leaking (i. V8. V37.e. Check pilot pressure (40 psi) path from the pneumatic drawer to the UFC. Return to Pneumatics Screen and monitor UFC and Main Vacuum. 2. Checks for sealing of the dome valves 1. If test fails again. 3. check for clogs in the waste lines from the UFC and in the lines to and from the waste manifold (behind power supply drawer). 1. this could cause vacuum problems. V7. Look for constrictions or leaks. 3. and V13. Close all valves and allow vacuum to recover. 3. Repeat test. V11. Check small vacushield filter for integrity. detector with bleach solution. 5. 4. replace the pilot valve. Verify that rinse is not seeping past the valve into the chamber. Replace large vacushield on the waste jug.PRELIMINARY PRODUCT INFORMATION Control Number: 024-0005-14 Revision: A U9 pin 8 & TP11-1 gnd Cond#2 = TP11-4 & TP11-1 gnd or U12 pin1 & TP11-1 gnd Threshold test points Cond#1 TP9-2 and gnd Cond#2 TP11-2 and gnd Mode 2B A voltage greater than –1v is detected at the analog test point but the system does not detect conductance. Restart test. Pressurize both rinse diaphragm pumps (V28 and V29) Observe suspect rinse valves (V2. V10. 7. Adjust 40 psi down to 30 psi Go to Exercisor. and system has an Auto-waste configuration. 4. Page 15 . Restart test. UFC Vacuum should not differ from Main vacuum by more than 5 in Hg as both vacuums drop. Access the Pneumatics Screen and verify UFC and Main Vacuum readings. Clean out the waste manifold. 2. Mode 2B Repeat as in mode 1B. V66). bypass the in-line check valve in the waste line to the waste jug. 6. 5. If pump is compromised. Monitor for at least 1 min. 2. Probe Clogs Failed Aspiration Rinse Dome Valve Sealing Test 1. Open V5. UFC Vacuum Test 1. as a last resort. Check fittings on waste jug including spigot and waste exit port. 4. rinse entering chamber during test) and it is not a dome valve failure. 2. 3. 8. 4. Note: Capacity of Vacuum Pump should be 24"-25" Hg. Go to Exerciser. Consult TTN before changing UFC. If test fails again. Close all valves and allow vacuum to recover.

Prime several times to allow bubbles to clear. 5. V6. then examine the reagent pump as it fills from the prime cycles. check for air leak or clogging in specific mode. block the light path. Go to Exerciser. Place fitting directly into reagent to see if filter on straw is the problem. 2. Retic .V13. If a small drop is 1. 1. Open the appropriate waste valve for the specific method: Perox -V5. 1. 2. Go to Exerciser. 2. For RBC/Baso/Retic problem us 50% bleach. Check vent lines for pinches. trace the line back to the line from the reagent pump to the shear face. 3. 4. Baso . check that the system ground strapping is proper. 2. Note: If imprecision is selectivity specific. 2. For Perox problem use E-Z Kleen. If not corrected go to next procedure.V65.Noise Caused by Improper Grounding 1.SINGLE METHOD COMPLAINT PATHWAY TESTED STEPS TYPICA L TIME RESULT CORRECTIVE ACTION [If Pathway Tested Fails] Imprecision for a Single Method 1. With the magnifying glass. 2. If the bubbles are seen rising up from the shear face. Run Saline samples in the Autosampler mode. Swap ends of the reagent line to the reagent pump and reagent bottle.Reaction Chamber / Vent for Specific Method 1. 2. If no bubbles are seen in #1 then clean the shear face. No air or bubbles should be seen in the reagent lines during prime cycles. examine the reagent delivery to the chamber. 2. remove reagent fitting from straw filter. Look for significant noise in the cytogram of the method in question. 1. Locate the sample delivery and reagent delivery line for the method in question. Exercise the reagent pump associated with the method while monitoring vacuum and Minimum noise should be seen in any channel. 3.PRELIMINARY PRODUCT INFORMATION Control Number: 024-0005-14 Revision: A TROUBLESHOOTING: IMPRECISION .Leak from 11-Position Manifold 1. If bubbles are injected. If noise is still present with the light path blocked. Repeat Precision after this test. RBC . Locate a magnifying glass or loupe. If noise is present. Cleans chamber pathway including vent and waste for effected method. Trace the reagent delivery back to the reagent bottle Prime the reagent line using the hydraulic prime cycles. Aspirate the appropriate fluid through the vent for the method and drain line. If bubbles are seen in # 1. check the reagent line for leaks. V7.Reagent Delivery for Specific Imprecise Method 1. If this does not correct. 3.V-10. 4. Repeat test. Should not see a significant drop in pressure or vacuum (>2-3 psi or >1-2"Hg). Repeat test. Page 16 . Access the 11 position manifold and pinch off the pressure vacuum line that goes to the reagent pump. 5. Check Perox cap for clog. 2. 4. 3.

Cross check by testing the valve in its new position. then swap the pneumatic valve with another position to isolate the problem to the valve itself.PRELIMINARY PRODUCT INFORMATION Control Number: 024-0005-14 Revision: A pressure readings from the pneumatic screen. Page 17 . If results are good. Large drop in pressure or vacuum and/or failure to recover indicates a leak associated with the 11 position manifold. pneumatic valve. it should recover while pump is still exercised. the leak is in the line to the pump. If the results are not good. or at tubing to specific pump or manifold. 4. 5. seen. 3.

Pressurize both rinse diaphragm pumps (V28 and V29) Observe suspect rinse valves for the method in question: (V2. Monitor for at least 1 min. If sample portion of the Hgb histogram is still good when running samples. replace the pilot valve. 4. 3. If there is one valve in particular that is leaking (i.e. as a last resort. 2. 2. # 1 could be due to Obstruction in Sample Line Troubleshoot this pathway # 1 could be due to Air Leak in Reagent Line Troubleshoot this pathway. V11. 5. Examine only the HGB precision. V8. 5. Look for constrictions or leaks. If precision is improved and "Flatness" is good. V66). re-run precision. 1. Run 10 to 20 sample precision. 4.SINGLE METHOD continued COMPLAINT PATHWAY TESTED STEPS TYPICAL TIME RESULT CORRECTIVE ACTION [If Pathway Tested Fails] Imprecision for a Single Method cont. 2. 2. Verify that rinse is not seeping past valve into the chamber. Turn the 20 psi down to 14 psi. Disregard all other parameters.PRELIMINARY PRODUCT INFORMATION Control Number: 024-0005-14 Revision: A TROUBLESHOOTING: IMPRECISION . 4. Checks for sealing of the dome valves 1. Hgb Imprecision 1. then violent mixing in the Hgb chamber is suspect.Rinse Dome Valve Sealing Test 1.5. 3. 3. Perform this test if no significant bubbles are observed in the reagent line and the transmission has a curve at the beginning of the sample portion of the Hgb histogram. 3. Page 18 . Go to Exerciser Adjust 40 psi down to 40 psi. Raise pressure to 19. If failure seen at 20 psi but improved at 14 psi then problem could be incorrect entry angle of sample. Check pilot pressure (40 psi) path from the pneumatic drawer to the UFC. V37. rinse entering chamber during test) and it is not a dome valve failure.

then replace it with a new one. Caused by Dome Valve 1. including the 11-position manifold. then go to loss of Vacuum caused by Dome Valve. 3. 2. Page 19 . then the indication is there is an electrical failure. Go to Exerciser.PRELIMINARY PRODUCT INFORMATION Control Number: 024-0005-14 Revision: A TROUBLESHOOTING . Check to see if Pilot valves vacuum supply (middle rear) has fluid in it. If the Valve on screen remains green while exercised. Note: these vacushield filters are designed to hold back moisture. If this test does not fail. If the Valve shows red.DOME VALVES. 5. Verify integrity of each diaphragm pump. If the Valve Driver swap does not correct. Note: If a leak is experienced in a diaphragm pump. open the valve for the suspected failure. Loss of Vacuum at UFC Caused by Diaphragm Pump Failure/Leak Diaphragm Pump Integrity 1. Note: Additional parts may have to be replaced if fluid did invade. 1. Check to see if there is fluid at the back of the UFC coupling (pull out Pnematics drawer to view) in the tubing from port 15 to the waste manifold. then the system is registering the correct electrical response. then any of the vacuum lines to the 11. REAGENT DIAPHRAGM COMPLAINT PATHWAY TESTED STEPS TYPICA L TIME RESULT CORRECTIVE ACTION [If Pathway Tested Fails] Loss of Vacuum at UFC Caused by Pilot Valve UFC Dome Valve and Pilot Valve integrity 1. Test to see if Pilot Valve is the problem for Dome Valve Failure. If a pump is found faulty and fluid has invaded the vacuum system. 6. 3.position manifold can have fluid in them since the vacuum supply is 1. 2. 4. vacuum head and pressure node board. 2. then replace the suspect pilot valve according to the procedure in the Service Guide. The vacuum lines involved will have to be dried out and vacushields replaced. then the small vacushields will be wet. If a large amount of fluid is experienced. 2. then the integrity of one of the dome valves is compromised and the UFC should be changed. verify that the pilot valve associated with this dome valve is functioning. Troubleshoot by swapping the Valve Driver Boards to see if the problem moves. If fluid is seen in the Pilot Valve vacuum line from the rear of the UFC. In the Valves Screen. then it will penetrate these filters No fluid should be seen in the vacuum/pressure lines from the diaphragm pump to the 11position manifold. No fluid should be seen in the Pilot valve vacuum line from the UFC to the UFC coupling 1. If a diaphragm pump is found to be faulty. vacuum regulator. If dome valve failure is suspected.

vacuum head and pressure node board. 1. No fluid should be seen in the vacuum/pressure lines from the diaphragm pump to the 11position manifold. Note: these vacushield filters are designed to hold back moisture. If a large amount of fluid is experienced. including the 11-position manifold. then the small vacushields will be wet.position manifold can have fluid in them since the vacuum supply is universal. then any of the vacuum lines to the 11. If a reagent pump is found to be faulty. 2. 3. Page 20 . Loss of Vacuum at UFC Caused by Reagent Pump Failure/Leak Reagent Pump Integrity Verify integrity of each reagent diaphragm pump. Note: If a leak is experienced in a diaphragm pump. Note: Additional parts may have to be replaced if fluid did invade. vacuum regulator.PRELIMINARY PRODUCT INFORMATION Control Number: 024-0005-14 Revision: A universal. 2. 1. If a pump is found faulty and fluid has invaded the vacuum system. The vacuum lines involved will have to be dried out and vacushields replaced. then it will penetrated these filters. then replace it.

Forward your suggestions to Pat Frank: By e-mail: Patricia Frank/TRYTN/DIAG/US/BAYER.PRELIMINARY PRODUCT INFORMATION Control Number: 024-0005-14 Revision: A CONTINUOUS IMPROVEMENT IDEAS Use this page to keep track of ideas for change as you think of them. Or Fax 1-91524-2249 Thank you for contributing! Page 21 .

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