DIZON, Anne Rose R.

December 10, 2012 2-BSM CMB Lab Experiment 2 Chemical Components of the Cell Water: Osmosis; Diffusion I. OBJECTIVE: To understand osmosis and diffusion in living cells. To understand the movement of solutions across a cell membrane II. PROCEDURE: 1. Boil 3 cups salt with 4 cups water. 2. Carefully place duck eggs in a wide container 3. Pour the brine solution into the container. Weigh down the eggs with a sealed plastic filled with water to keep them from floating. 4. Keep the container in a cool dry place. 5. Try one egg after 14 days. Soak the eggs again for 5 days more if the dried e gg is not salty enough. 6. When the eggs are salty enough boil them for 10 minutes. , III. RESULTS: Observation: November. 29, 2012 *13th day of the duck egg soaked in the salt solution. Characteristics Egg white Egg yolk Taste The saltiness is moderately normal It s not that salty but rather rich and full of flavor Texture The surface is smooth but not soft The yolk is gritty in texture Consistency The egg white is firm The consistency of the yolk is soft yet gritty Color The color is white The yolk is yellow orange and quite oily IV. DISCUSSION: From the salt curing process, the salted duck eggs are not too salty, have a bri ny aroma, a firm-textured egg white, egg yolk that is bright orange in colour an d it produced oil. For the group, there is no remedial action made because the s altiness of the duck egg is just right. V. CONCLUSION: I therefore conclude that water will pass through the shell of an egg to the sid e that has a solution of higher concentration. Hence, osmosis is very important for living organisms. Cells use this process to pull in and expel water from wi thin the cell walls. The walls of the cells act as the semi-permeable membrane. The membrane allows smaller molecules like water to pass through but prevents la rger organic molecules from leaving the cell.

VI. Questions: 1. Differentiate osmosis, diffusion and dialysis? Osmosis is a process in which a fluid passes through a semipermeable membrane, m oving from an area in which a solute such as salt is present in low concentratio ns to an area in which the solute is present in high concentrations. The end res ult of osmosis, barring external factors, will be equal amounts of fluid on eith er side of the barrier, creating a state which is known as isotonic. Diffusion is the movement of particles (atoms, ions or molecules) from a region in which they are in higher concentration to regions of lower concentration. Dialysis is the separation of substances in solution by means of their unequal d iffusion through semipermeable membranes. 2. Differentiate hypertonic, hypotonic and isotonic solution? Cells hypertonic to their surrounding solutions cause water to move into the cel l and cause it to expand. Cells hypotonic to their surrounding solutions cause water to move out of the ce ll and cause it to shrink. Cells isotonic to their surrounding solutions have an equal concentration of sol utes in and out of the cell membrane. 3. Base on the salt concentration, what kind of solution did you use? Hypertonic , hypotonic or isotonic and Why? The group used hypertonic solution because the egg shell is permeable, salt ente rs and water is also removed from the egg in this process. 4. What will be the effect of the hypotonic and hypotonic solution on a living c ell? When a cell has a smaller number of solutes than the solution outside of the cel l membrane, its effect can be seen when a plant wilts. In animal cells, the memb rane lose shape and support, shrivel up, and die. While, when a solution is said to be hypertonic, it causes turgor pressure in plants that make the plants rigi d for support. In animal cells, this process can cause the cell membrane to burs t and die. 5. What is the effect of isotonic solution on a living cell? Since an isotonic solution has an equal concentration of solutes in and out of t he cell membrane, it creates a dynamic equilibrium in a cell that maintains the status of the cell. Name:____________________________ Date:_____________ Year & Section:____________ CMBLAB Experiment 2 Chemical Components of the Cell Water: Osmosis; Diffusion I. Objective: (make your own)

II. Summary of the procedure: ( make your own) III. Results: a. Visual (appearance) b. Sensory (Taste) Rate of Saltiness Blunt Slightly salty Medium or moderate salty Very Salty c. Remedial Action No remedial action IV. Discussion (Things to write here what action did you do after tasting? Was it too salty? Mo derate? Also write here no remedial action for our group) V. Conclusion VI. Questions: 1. Differentiate osmosis, diffusion and dialysis? 2. Differentiate hypertonic, hypotonic and isotonic solution? 3. Base on the salt concentration, what kind of solution did you use? Hypertonic , hypotonic or isotonic and Why? 4. What will be the effect of the hypotonic and hypotonic solution on a living c ell? 5. a. What is the effect of isotonic solution on a living cell? b. What is the effect of isotonic solution on a living cell? VII. References: VIII. Documentation (Photographs): (to be submitted as a group at the last page) ***ako na magpriprint