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JIWAJI UNIVERSITY, GWALIOR – 474011

MASTER OF SCIENCE (BIOCHEMISTRY) CURRICULUM (2012-2014)
Semester First Title of the Paper (s) 101. 102. 103. 104. 105. 106. 201. 202. 203. 204. 205. 206. Third 301. 302. 303. 304. 305. 306. Fourth 401. 402. 403 404 Frontier Technologies in Biosciences Biostatistics, Computers and Bioinformatics Lab Course VII Project Work* 85 85 100 100 15 15 Genetic Engineering Enzyme Technology Animal Physiology & Endocrinology Nutritional and Clinical Biochemistry Lab Course V Lab Course VI 85 85 85 85 100 100 15 15 15 15 Cell Biology Biomolecules Microbiology Plant Biochemistry Lab Course I Lab. Course II Molecular Biology Immuno Technology Tools and Techniques Bioenergetics and Metabolism Lab Course III Lab Course IV Max Marks EA IA 85 15 85 15 85 15 85 15 100 100 85 85 85 85 100 100 15 15 15 15 -

Second

*Evaluated both by the Internal & External examiner at the time of presentation. There are weekly seminars and continuous internal assessment throughout the course.

101: CELL BIOLOGY
UNIT I 1. Cell Membrane: Physicochemical Properties; Molecular Organization – organization of lipids, proteins and carbohydrates; Biogenesis and Functions 2. Transport of Small Molecules Across Cell Membranes: Types and Mechanism 3. Active Transport by ATP-Powered Pumps: Types, properties and mechanisms 4. Transport of proteins into mitochondria and chloroplast UNIT II 1. 2. 3. 4. Transport of proteins into and out of nucleus Transport of proteins into endoplasmic reticulum Transport by vesicle formation: Endocytosis and Exocytosis Molecular Mechanism of vesicular transport asymmetrical

UNIT III 1. Intracellular Digestion: Ultra structure and Functions of Lysosomes 2. Cell Motility and Shape I: Structure and Functions of Microfilaments 3. Cell Motility and Shape II: Structure and Functions of Microtubules and Intermediate Filaments 4. Intracellular communication through cell junctions: Occluding junctions, Anchoring Junctions and Communicating Junctions UNIT IV 1. 2. 3. 4. Molecular Mechanism of Cell-Cell Adhesion: Ca++ dependent cell-cell adhesion Molecular Mechanism of Cell-Cell Adhesion: Ca++ independent cell-cell adhesion Extra-cellular Matrix of Animals: Organization and Functions Extra-cellular Matrix Receptors on Animal Cells: The Integrins

UNIT V 1. Cell Signaling: Signaling via G-Protein linked and enzyme linked cell surface receptors, MAP Kinase Pathways, Interaction and Regulation of signaling pathways 2. Eukaryotic Cell Division Cycle: Different Phases and Molecular Events 3. Control of Cell Division Cycle: In yeast and mammalian cells 4. Apoptosis: Phases and significance, Morphological and Biochemical changes associated with apoptotic cells, Apoptotic Pathways and regulators Practical Exercises 1. Sub cellular fractionation 2. Chromosome Preparation: Mitosis – Onion root tip, rat/mouse cornea, rat/mouse bone marrow, human lymphocytes 3. Chromosome Preparation: Meiosis – Rat/mouse testis, Grasshopper testis 4. Polytene chromosome preparation from Drosophila salivary gland 5. Identification of tissue typing: Histological preparation of tissue 6. Identification of different biomolecules in different tissues by histochemical techniques 2

et al 3. Working with Molecular Cell Biology: A study Companion. The Cell: A Molecular Approach. 3 . Electron microscopy: Demonstration and good photographs for interpretation. Molecular Biology of the Cell. Essential Cell Biology. Cell Proliferation and Apoptosis. Storrie et al 4. Cooper 6. Biochemistry and Molecular Biology of Plants. et al 2. Reference Books 1. G.7. Alberts et al 9. Buchanan et al 10. Harpers Biochemistry Murray et al Note: All text books are of latest editions. Lodish. Gerald Karp 5. Becker et al 7. Hughes and Mehnet 8.M. Alberts. The Word of the Cell. Molecular Cell Biology. Cell and Molecular Biology: Concepts and Experiments.

4. classification. degradation. classification. modification. 6.Zubay 4 . repair. 2. tertiary and quaternary structure UNIT III 1. properties and functions Lipids with special biological functions UNIT II 1. 4. Enzyme kinetics : Basic concepts 3. Enzymes : Classification. properties and functions Peptides and polypeptides Synthesis of peptides and protein sequencing Proteins : Properties. Biochemistry by G. Carbohydrates : Structure. Animal hormones : Structure and biological roles Plant hormones : Structure and biological functions Plant phenolics: Classification and functions Alkaloids : Classification and functions Practical Exercises 1. Principles of Biochemistry by Nelson. 2. 3. nucleotides DNA : Structure and Conformation DNA : denaturation. Cox and Lehninger 2. saponification value. structure. covalent structure. 3. 4. sugars and lipids Quantitation of proteins and sugars Analysis of oils : iodine number. 3. multienzyme complex 2. Water soluble vitamins : Structure. 3. distribution and functions UNIT III 1. distribution. 2. nucleosides. mechanism of action. secondary. 4. 4. 5. acid number Reference Books 1. types and functions UNIT V 1. Titration of amino acids Colorimetric determination of pKa Model building using space filling/ ball and stuck models Reaction of amino acids. 2. allosteric enzymes. 3. Amino acids : Structure. Fat soluble vitamins : Structure. Nucleotides : Structure of purine and pyrimdine bases. properties and functions Home and heterpolysaccharides : carbohydrate derivatives Lipids : Classification. interaction and biological functions (mechanism of action not included) 4.102: BIOMOLECULES UNIT I 1. 2. recombination and rearrangement RNA : Structure. abbreviations.

W. Note: All texts are of latest editions. Voet . DVoet and JG. 5. Biochemistry. 9. 4. Biochemistry by Stryer Biochemistry by Garrett and Grisham Biochemical Calculations. Freeman & Company. 8. J Wiley and Sons. Wilson & Goulding. John Wiley and Sons Inc. 5 . Work and Work A Biologist’s guide to Principles and Techniques of Practical Biochemistry. Seigel. Laboratory Techniques in Biochemistry and molecular Biology.3. ELBS Edition. 7.H. Irwin H. 6. D Freifilder. Biochemistry.

Mycoplasmas and diseases caused by them Bacterial Recombination: Transformation. and Poliomyelitis UNIT V 1. Mathematical expression. Classification of Microorganisms: Basis of microbial classification. Isolation. Haekel three kingdom. Preparation of Culture media. Aspergillosis Viral diseases: Chicken pox. Pure culture techniques and preservation methods. 2. 3. streaking and serial dilution methods. measurement of Growth and factors affecting growth 3. 3. common nutritional requirements. 2. Measurement of bacteria population by turbidometry 6 . Storage of microorganisms Isolation of pure cultures from soil and water. Morphology and fine structure of eubacteria and archeobacteria cell wall.103: MICROBIOLOGY UNIT I 1. Growth. Salmonellosis Shigellosis Fungal diseases: Histoplasmosis . UNIT III 1. 4. 4. 2. 4. Slants and stab cultures. cultivation. Hepatitis B. transport of nutrients across the bacterial membrane 4. Conjugation. types of Infection. Oxygen toxicity: Study of catalase. mode of nutrition. superoxide dismutase. Microbial Nutrition: Nutritional classification of Microorganisms. identification and viral replication. Lytic and lysogenic cycle. Structure and morphology of Bacteriophage. Preparation of liquid and solid media for growth of microorganisms. Mechanism of pathogenicity Bacterial Diseases: Staphylococcal and Clostridial food poisoning. peroxidase. Isolation and maintenance of organisms by plating. Life cycle of DNA Viruses: SV 40. mode of action of antibiotics and resistance to antibiotics Practical Exercises 1. 2. Transduction. Broad spectrum antibiotics Antibiotics from prokaryotes Anti-fungal and antiviral antibiotics. cytoplasmic membrane and other organelles. 3. Whittaker’s five kingdom concept. Virus: Types. Plasmids and transposons Chemotherapeutic agents: Classification of antibiotics. 2. 4. UNIT II Sterilization: Physical and chemical methods Microbial Growth: Bacterial growth curve. 3. 3. Growth curve. 2. Infection and disease. 1. RNA Viruses: Retroviruses Cynobacteria : General account and their importance UNIT IV 1. microbial staining. 4. mechanism of oxygen toxicity.

Biochemical characterization of selected microbes. General Microbiology. H. Addison Weseley. 11. M.Jr and Freifelder. Yeast and molds and study of organisms by Gram stain.J. and Painter. 10. Microbiological Applications.. Wheelis. Prentice-Hall.R.T.L. Analysis of water for portability and determination of MPN.M. 5. Martinko.G.S. J.L.and serial dilution methods. Pelzar. Maloy . Bacterial transformation. Bartlett Publishers. 9. J. Jones.R.a Laboratory Manual. N.cappuccino. P. Microbiology.. Cronan. J. Microbial Genetics. N. 6. The MacMillan Press Ltd. 7. Study of mutations by Ames test. Reference Books 1. WCB: Wm C. (A Laboratory Manual in General Microbiology) Benson. S. R.Y. D. and Sherman.E. Tata McGraw Hill. and Parker. E. and Kreig. 6. Microbiology. 4. One step growth curve of coliphage. 2.. J.C. M. Brown Publishers Note: All text books are of latest editions 7 . Microscopic examination of bacteria.J. 5. pH and carbon and nitrogen sources on growth. M. Effect of temperature. Chan . 3. Ingraham.R. 8. Brock Biology of Microorganism. Acid fast stain and staining for spores. J. Assay of antibiotics and demonstration of antibiotic resistance.

light harvesting complexes. role of light in activation of dark phase enzymes Photorespiration: Mechanism and regulation The C4 mode of photosynthesis: Mechanism. ferrodoxin. nif genes and their regulation. UNIT II 1. 2. Biological Nitrogen Fixation: Formation of ammonia. proton gradient and electron transfer in chloroplasts of plants and in purple bacteria-difference from mitochondria Light receptors.Location. distribution. 4. assimilation and secondary assimilation of ammonia.Evidence. 3. biological functions Plant phenolics : nature. lignins and tannins. adsorption and transport of water and ions in plants Translocation of inorganic and organic substances Structure and biogenesis of organelles involved in photosynthesis in plants 2. xanthones.Distribution. The Calvin Cycle. 2. Secondary metabolites: Plant alkaloids. bacteriorhodopsin as ion pump Photosystem I and II. stilbenes. 4. conversion of nitrate to ammonia. 4. mechanism and stoichiometry. applications of biological nitrogen fixation Molecular effects and mechanism of action of Auxin and Gibberellic Acid Molecular effects and mechanism of action of Auxin and Gibberellic Acid UNIT V 1. flavanoids. primary and secondary cell walls. 2. 3. localization and biosynthesis of true proto and pseudoalkaloids. Estimation of plant proteins 8 . 2. 3.Chlorophyll. Chloroplast membrane and molecular organization of thylakoids. biosynthesis and regulation of phenolics. 3. difference from C3 in relation to plant productivity Crassulacean Acid Metabolism: Mechanism and regulation UNIT IV 1. Specialized plant organelles : Cell plate. phenylpropenes. bioflavanoids.104: PLANT BIOCHEMISTRY UNIT I 1. Plasmadesmata. hydroxycinnamic acid. stoichiometry and purpose. Plasids. plastocyanin. biosynthesis. mechanism of energy transfer between pghotosystems. Importance of vacuoles and microbodies. biological functions Biochemistry of seed development and fruit ripening Defense system in plants Practical Exercises 1. Meristematic cells an root quiescent zone Absorption. inhibitors Molecular properties of nitrogenase system. quinines.Types and functions. 3. 4. plastoquinones and carotenoids Hill reaction and photophosphorylation UNIT III 1. Cell wall. phenolic acids.Chemical and physical composition. 4.

6. 3. Estimation of plant lipids and carbohydrates Isolation of plant pigments.in vivo method Fruit ripening Estimation of total phenolic compounds Estimation of anthrocyanin pigments Reference Books 1.2. 3. their analysis and determination of absorption Maxima Chloroplast isolation Hill Reaction Estimation of nitrogenase Estimation of nitrate reductase. 4. 4. 9. 2. 5. 10. 7. Handbook of Photosynthesis by Mohammad Pe Sarakle Plant Physiology by Salisburry and Ross Introduction to Plant Biochemistry by Goodwin and Mercer Seed: Physiology of Development and Germination by Bewley and Balck Biochemistry of Energy Utilization in Plants by Blakie Plant Biochemistry by Dey and Harbome Note: All text books are of latest editions 105 : Lab Course I Consists of practical exercises listed out under 101 & 102 106 : Lab Course II Consists of practical exercises listed out under 103 & 104 9 . 5. 8. 6.

Regulation of gene expression at translational level Transposable elements in Prokaryotes and Eukaryotes: Types and significance Oncogenes and Tumor Suppressor Genes: Properties and significance Post-transcriptional Processing of pre mRNA.201: MOLECULAR BIOLOGY UNIT I 1. attenuation and antitermination Nature of Gene : Evolution of Gene Concept. 3. Regulation at the level of processing of transcripts Regulation of Gene Expression in Eukaryotes: RNA Editing. 2. 2. 3. Translational adaptors and amino acyl tRNA synthetases Translation: Successive stages of protein synthesis in prokaryotes and its comparison with eukaryotes Post-translational Modification: Types and Significance Regulation of Gene Expression in Prokaryotes : Operon concept. 2. Enhancers – Structure and properties. Response Elements Post-transcriptional Modification Eukaryotes – 5’ and 3’ modification of mRNA Transcription in Prokaryotes : Initiation. 4. DNA Replication Machinery in Prokaryotes and its comparison with Eukaryotes Enzymology of DNA Replication : DNA Polymerases. Gene-cistron relationship in prokaryotes and eukaryotes DNA Replication : General features of Chromosomal Replication. induction and repression. 4. pre rRNA and pre tRNA transcripts Genetic Code : Evidence and properties. 2. 2. Regulation of gene expression by hormones. Gene Alteration. 3. structure and properties Transcription factors – Types and properties. Ligases. DNA methylation and gene regulation. Helicases. Primases. Topoisomerases. 3. 4. 4. 3. Regulation of Gene Expression in Eukaryotes: cis-acting DNA Elements. Promoter – Types. 4. Chemical Nature of Gene. UNIT IV 1. Structure and regulation of lactose. Wobble hypothesis. arabinose and tryptophan operons Initiation of Transcription in Eukaryotes : RNA Polymerases – Types and properties. Chromatin organization and regulation of gene expression. elongation and termination Structure and functions of prokaryotic promoter Control of transcriptional initiation in prokaryotes : Structure and functions of RNA Polymerase. Sigma factors – Types and functions Control of transcriptional termination in prokaryotes : Intrinsic termination and Rho factor dependent termination. UNIT III 1. Gyrases and Single Stranded Binding Proteins Regulation of DNA Replication 10 . UNIT II 1. UNIT V 1.

DNA interaction Reference Books 1. 4. Determination of Amax of purified DNA fragments 5. Quantitation of DNA 4. by Lea and Leegood Plant Biochemistry. 2. Metabolic labeling of proteins and immunoprecipitation 11. by Lodhish et al Biochemistry and Molecular Biology of Plants. by Griffiths et al Genome. Isolation of genomic DNA and restriction digestion 2. by Turner et al Cell and Molecular Biology: Concepts and Experiments. 5. Size fractionation of restricted DNA fragments by Agarose Gel Electrophoresis 3. 3. by Klug and Cummings Proteins. 8. 9. 11. by Carey and Smale Translational control of gene Expression. by Turner An Introduction to Genetic Analysis. 7. In vitro translation 10. 12. by Benjamin Lewin Molecular Biology. In vitro transcription 9. by Gerald Karp Transcriptional Regulation in Eukaryotes. Genes VIII . by Sonenberg et al Chromatin and Gene Regulation. by Brown Concepts of Genetics. by Buchanan Plant Biochemistry and Molecular Biology.Practical Exercises 1. 6. Determination of Tm of nucleic acid 6. 10. 13. by Dey and Harborne Note: All text books are of latest editions. 11 . Isolation of RNA 7. 14. by Creighton Molecular Cell Biology. Protein. Fractionation of poly (A)+ RNA 8.

types and functions. Super antigens 4. its mediators and the process. properties of T and B Cell epitopes. granulocytes in inflammatory process UNIT II 1. Techniques of Cell mediated immunity Immunoregulation mediated by antigens. Organization and arrangement of T-receptor genes 4. and regulation of activation pathways. Complement deficiencies. 4. activation pathways. Anatomical organization of immune system: Primary lymphoid organs. lymphocyte homing. 3. cytokine related diseases and therapeutic applications of cytokines Cytotoxic T cells and their mechanism of action. Immune response: Innate immune mechanisms and characteristics of adaptive immune response. Antigen-Antibody interaction. Secondary lymphoid organs.202: IMMUNOTECHNOLOGY UNIT I 1. Immunoglobulins: Molecular structure. Molecular mechanism of antibody diversity: Organization of genes coding for constant and variable regions of heavy chains and light chains. MHC and cytokines Hypersensitivity: Definition. Hematopoiesis 2. T-cell accessory membrane molecules.I reactions and consequences. Cell of the immune system: Mononuclear cells and granulocytes. polymorphism. avidity & affinity measurement Monoclonal antibodies: Production. IgE mediated hypersensitivity. role of anaphylatoxins. tissue injury and immune response leading to an inflammatory reaction. therapy and basic research Complement system. Type II reactions. Antigens. mediators of type. distribution variation and function. components. Antigen presenting cells. Activation of B-cells. 2. Lymphocyte traffic 3. Cytokines: Structure and functions. Ontogeny and Phylogeny of lymphocytes. B-cell receptor complex. Antigenic determinants on immunoglobulins UNIT III 1. Class switching Antibody engineering. Antibody dependent cell mediated cytotoxicity. 3. cytokine receptors. signal transduction mediated by cytokine receptors. Heptanes: Factors affecting immunogenicity. characterization and applications in diagnosis. Lymphocytes and their subsets. Association of MHC with disease 2. Major histocompatibility systems: Structure of MHC I and II molecules. Role of MHC molecules in antigen presentation and Costimulatory signals 3. Mechanisms of antibody diversity. immune complexes. mechanism of mast cell degranulation. T – cell receptor complex. Organization of MHC complex in Mouse and Humans. antibodies. activation of T cells. NK cells and mechanism of target cell destruction. 4. Cell adhesion molecules and their role in inflammation. Immune complex mediated hypersensitivity and Delayed type hypersensitivity 12 . 2. Inflammation. cytokine regulation of immune responses. Recognition of antigens by T and B Cells: Antigen processing. Role of complement system in immune responses UNIT IV 1. Delayed type hypersensitivity.

5. ELISA. Immunology. Agglutination. 7. Immunodeficiency syndromes: Primary immunodeficiencies and Secondary immunodeficiencies and their diagnosis and therapeutic approaches 3. Western blotting and Immno-histochemical techniques Practical Exercises 1. DNA Vaccines. Separation of mononuclear cells by Ficoll. 11. Kubey. Immunology. -Eli Benjamini. 4. Vaccines: Active and passive immunization. A. Immunization and production of polyclonal antibodies Double diffusion and Immuno. 4. Immunology by Roitt et al Immunology by Abbas Note: All text books are of latest editions. Lymphoid organs and their microscopic organization.UNIT V 1. Thomas J. 6. 2. 5. Con-A induced proliferation of thymocytes (by MTT method).A. 6. Geoffrey Sunshine.Ashort Course. Macromolecules as vaccines.Hypaque. Immunodiagnostics : Precipitation techniques. Synthetic peptide vaccines and sub-unit vaccines 4. Richard Coico. Blood Film preparation and identification of cells. 8. Mechanisms of autoimmunity and therapeutic approaches 2. 3. 9. Fluorescence techniques. 3. 2. RIA. (Freeman). Autoimmunity: Organ specific diseases. Recombinant-vector vaccines. R. Systemic diseases. Radial Immunodiffusion.electrophoresis. ELISA Preparation of antibody-enzyme conjugates Reference Books 1. 10. Goldsby. Kindt. Whole organism vaccines. Western –blotting. Barbara. 13 . Immunology by Tizzard Fundamentals of immunology by William Paul. Osbarne. Purification of IgG from serum.

type. Atomic absorption spectroscopy: Principle.203: TOOLS AND TECHNIQUES UNIT I 1. 4. interference. 2. 2. instrumentation and applications ORD and CD UNIT IV 1. instrumentation and applications X ray crystallography: Principle. instrumentation and applications Photometry: Basic principles of colorimetry. 2. 2.native and under denaturing conditions. Physical Biochemistry: Applications to Biochemistry and Molecular Biology by Freifelder 14 . 6. instrumentation and applications Infra red spectroscopy Fluorimetry: Principle. instrumentation and applications Flame emission spectroscopy: Principle. gamma counter Autoradiography: Principles. instrumentation and applications Polarimetry: Principle. instrumentation and applications Radio immunoassay: Basic principle and applications UNIT V 1. GC Mass: Basic principle. HPLC and FPLC Electrophoresis: Principle. 3. 3. 4. Centrifugation: Basic principle. liquid scintillation counter. 5. 3. ESR: Principle. Microscopy: Light. instrumentation and applications NMR. instrumentation and applications Affinity chromatography. solid scintillation counter. 4. Geiger Muller Counter. 3. 4. Chromatography: Principle. fluorescence and polarization microscopy Electron microscopy: Principle and Applications Radioactivity: Principle. 4. Amino acid and carbohydrate separations by paper & thin layer chromatography Gas chromatography Ion exchange and gel filtration chromatography Separation of blood cells by density gradient centrifugation Reference Books 1. types. 4. instrumentation and applications UNIT II 1. types and applications Isoelectricfocussing and isotachophoresis UNIT III 1. and UV visible spectrophotometry. phase contrast. 3. 7. 3. 2. 2. and applications Practical Exercises 1. Verification of Beer’s law Determination of absorption maxima Electrophoresis of Proteins.

8. 15 . 5. 3. Biochemical Techniques: Theory and Practice by Robyt and White Principles of Instrumental Analysis by Skoog and West Analytical Biochemistry by Holme and Peck Biological Spectroscopy by Campbell and Dwek Organic Spectroscopy by Kemp A Biologist’s Guide to Principles and Techniques of Practical Biochemistry by Wilson and Goulding Principles of Instrumental Analysis by Skoog. 7. Hollar and Nicman Note: All text books are of latest editions. 6.2. 4.

3. 4. 2. glyconeogenesis. 3. UNIT V 1. 4. 4. To observe the catabolism of carbohydrates by micro-organisms To observe the production of gas by micro-organisms during fermentation To demonstrate the production of pyruvate and acetaldehyde during fermentation of glucose by yeast To demonstrate biological oxidation and electron transport in heart muscle tissue To observe the effect of fasting on the metabolism of rats ______________________________________________________________ 205 : Lab Course III Consists of practical exercises listed out under 201 & 202 206 : Lab Course IV Consists of practical exercises listed out under 203 & 204 16 . functions of ATP Other high energy biological compounds Practical Exercises 1. glycogenesis. Inborn errors of amino acid metabolism Nucleic acid metabolism Inborn errors of nucleic acid metabolism Integration of metabolism and metabolomics Nitrogen assimilation Biosynthesis of amino acids Degradation of amino acids Regulation of amino acid metabolism Electron transport and oxidative phosphorylation Biosynthesis and degradation of lipids Regulation of lipid metabolism Inborn errors of lipid metabolism Basic Concepts of intermediary metabolism Carbohydrate metabolism: Glycolysis. UNIT IV 1. pentose phosphate pathway. UNIT II 1. 4. 3. 2. Kreb’s cycle. UNIT III 1. 4. 2. 5. 2. 3. 4. 2. glycogenolysis. 3. glyoxalate pathway Regulation of carbohydrate metabolism Inborn errors of carbohydrate metabolism First and second laws of thermodynamics Concept of free energy ATP Cycle. 3. 2.204: BIOENERGETICS AND METABOLISM UNIT I 1. ATP as high energy compound.

insertion and point mutation UNIT V 1. advantages and disadvantages of different types of libraries. adenovirus. RNA Dependent DNA Polymerase. DNA polymerase I. bovine papilloma virus. Principle and applications of Gel Mobility Shift Assay. deletion. Polymerase Chain reaction. Mutant construction: Introduction. The recombinant DNA Technology : General concept and Principle of cloning 2. Methods for determination of copy number of a cloned gene in genome 4. Methods for screening and selection of recombinant clones 3. Cloning in animals system: Animal system as a model host. vaccinia virus. poly A polymerase 3. Oligonucleotide synthesis. phosphomonoesterases. Vectors for cloning in animal system – SV 40. bacteriophage and plasmid-phage) 4. Different methods for constructing genomic and full length cDNA libraries 4. DNA Fingerprinting and DNA Foot printing. Enzymes: Nucleases and restriction endonucleases – properties and types. Cloning in Yeast: Properties of yeast as host for cloning and different types of vectors designed for cloning in yeast 4. Principles and applications of Blotting techniques – Southern. in-situ hybridization. vectors for cloning in E. Chromosome mapping and chromosome painting Applications of Recombinant DNA Technology in Medicine Biosafety measures and Regulations for genetically engineered products 17 . 2. coli (plasmid. site directed mutagenesis. Design and characteristics of expression vectors for cloning in prokaryotes 2. terminal deoxynucleotidyl transferase. Methods for Constructing rDNA and cloning: Inserts. Methods of introduction of foreign DNA in animal system. transfection and cloning 2. baculovirus and retrovirus vectors UNIT III 1. coli as host. DNA ligase. Gross anatomy of cloned insert – size. Factors affecting expression of cloned genes in prokaryotes 3. Restriction fragment length polymorphism. Northern. 3. Prokaryotic host-vector system: Characteristics of E. Other Prokaryotic host vector systems: Characteristics of Gram positive and Gram negative organisms as host and suitable vectors for cloning. Localization of cloned segments in genomes – molecular and chromosomal location 3. infection. 2. Western and South-Western.301: GENETIC ENGINEERING UNIT I 1. vector insert ligation. 4. DNA Libraries: Types. Shuttle Vectors UNIT II 1. restriction mapping and location UNIT IV 1. polynucleotide kinase. Fine anatomy of DNA segment – General principle of chemical and enzymatic methods of nucleotide sequence analysis and advantages of automatic gene sequencers.

Quantitation of nucleic acids 5. Dale Molecular Cloning (Vol I. Restriction mapping of plasmid DNA 6. 4.coli and analysis of gene product 10. Isolation of phage DNA 4. 8. II & III). Preparation of single stranded DNA template 9. 2. Recombinant DNA – By Watson et al Principles of Gene Manipulation. Preparation of helper phage and its titration 8. by Sambrook & Russell Applied Molecular Genetics. by Old and Primrose Gene Cloning: An introduction. by Singer & Berg Molecular Biotechnology. by Brown Biotechnology: Theory and Techniques (Vol I & II). Isolation of plasmid DNA 3. by Glick & Pasternak Plant Molecular Biology (Vol I & II). 3. Polymerase Chain Reaction Reference Books 1. Preparation of competent cells 2. by Chirikjian Molecular Genetics of Bacteria . 7. 5. 6. 10. Bacterial Culture and antibiotic selection media. Gene expression in E . Cloning in plasmid/phagemid vectors 7. by Miesfeld Genes and Genome. by Gilmartin & Bowler Note: All text books are of latest editions: 18 .Practical Exercises 1. 9.

Metallo-enzymes 4. EC number. Protein. method of isolation. 3. 6. Enzymes during aging Practical Exercises : 1. Ribonuclease. Lysozyme. Allosteric enzymes : Models of allostery. Ion exchange chromatography. Cofactors and Isoenzymes UNIT III 1. 8. specific activity 19 . classification and nomenclature. Hill and Scatchard plot. 4. temperature and pressure Enzyme inhibition: reversible and irreversible inhibition. molecular sieve chromatography.302: ENZYME TECHNOLOGY UNIT I 1. coupled enzyme assay and methods and significance of enzyme turnover number. Urease estimation in plant tissues Assay of Acid phosphatase in plant seeds Assay of Alkaline phosphatase in Kidney and Liver Determination of optimum pH. stable storage of enzymes 3. Principle and Industrial application of immobilized systems 3. techniques. experimental procedures and effect of immobilization on kinetic parameters 2. Mechanism of action of Chymotrypsin. Characterization of purified enzyme 4.ligand binding: types. purification and crystallization 2. methods homogenization. Degradation of enzymes UNIT V 1. cooperativity. Regulation of enzymes 3. inhibitor constant and its significance Rapid reaction techniques of Enzyme: Historical aspects. types and kinetics 2. Enzyme immobilization. 2. their type. temperature & time Determination of Km value of alkaline phosphatase Acetylcholinesterase estimation in Rat /Goat Brain Enzyme purification: Ammonium sulphate precipitation. 2. Bradford and Spectrophotometeric. 4. Enzymes in Medical diagnosis and enzyme therapy 4. rate equation and determination of Km and Vmax Factors affecting rate of enzyme reaction: pH. 7. 3. UNIT II 1. 3. Enzyme Kinetics: Equilibrium and steady state theory. UNIT IV 1. 2. Mechanism of enzyme catalysis and action Sub cellular localization and organization of enzymes Methods of enzyme assay: continuous and sampling techniques. Criteria of purity and tabulation of purification data. 5. 4. Coenzymes. Enzyme purification techniques: objectives and strategy. Protein estimation methods: Lowry.

L. Singh Note: All text books are of latest editions. Molecular weight determination of enzyme by Gel Filtration 11. 6. 12. 5. Tripathi Practical Biochemistry by Plummer. Practical Biochemistry by Sawhney and R. Sub-cellular fractionation of rat liver and marker enzyme assays. Foster Enzymes by Dixon and Webb Fundamentals of Enzymology by Price and Stevens Enzyme Catalysis and Regulation by Hammes Enzyme Reaction Mechanisms by Walsch The Enzymes vol I and II by Boyer Enzyme Structure and Mechanism by Alan Fersht Enzyme Assays: A Practical Approach by Eisenthal and Danson Enzyme Biotechnology by G. 20 . Reference Books 1. 9 10 11 The Nature of Enzymology by R. 4. Immobilization of HRP (Horse reddish peroxidase). 3. Checking of purity of enzyme by PAGE 10. Kinetic properties of Immobilised HRP 13. 7. 8.9. 2.

metabolism and functions Hormones of the pancreas: structure. 4. synthesis. secretion. functions and biogenesis of RBC Functions of hemoglobins. metabolism and functions Practical Exercises : 1. Nerve impulse transmission: structure of neuron. energetics of muscle contraction. inhibitors of coagulation UNIT II 1. 2. metabolism and secreted by parathyroid Hormones of the thyroid structure. secretion. To To To To To determine Hb% by Sahli’s hemometer in blood samples determine the hematocrit determine the concentration of heparin in blood samples determine the PTT in blood samples demonstrate the effect of diet and hormones on the glycogen content of rat liver 21 . lymphatic systems) Mechanism of blood coagulation : extrinsic and intrinsic pathways. plasma proteins Circulatory System (Open and closed circular. transport. neurotransmitters Presynaptic and post-synaptic events of neuromuscular junctions Ultra structure and molecular mechanisms of contraction of skeletal muscles and its regulation. 2. transport. secretion. transport. secretion. synthesis. synthesis. secretion. secretion. 5. receptors hormones Structure. metabolism and secreted by the pituitary Structure. 2. transport. 4. functions and mechanism of actin of function of the hormones function of the hormones transport. 2. relaxation Contraction of smooth muscles UNIT IV 1. synthesis. metabolism and UNIT V 1. 4. structure. 4. 2. 3.303: ANIMAL PHYSIOLOGY AND ENDOCRINOLOGY UNIT I 1. 3. 4. 3. synthesis. transport. 2. 3. Hormones of the adrenal medulla. Composition of Blood: Structure. secretion. transport. General characters and classification of hormones. synthesis. mechanism of conduction of nerve impulse along axon. 4. metabolism and functions Hormones of the testis and ovary: structure. 3. Urine formation: Formation of dilute and concentrated urine Regulation of water electrolyte balance and role of kidney and hormones Acid-base balance and its regulation by kidney and hormones Digestive system UNIT III 1. synthesis. metabolism and functions Hormones of the adrenal cortex: structure. 3.

Reference Books 1. Physiology by Guyton Medical Physiology by Best and Taylor Physiology by Garrett Harper’s Reviews of Biochemistry Note: All text books are of latest editions. 3. 4. 2. 22 .

4. Role of kidney in biochemical processes. Basic concept: Concept of accuracy. Nutritional and clinical significance of dietary calcium. 4. Kidney. Definition of BMR and SDA and factors affecting these. 3. role of antigen. Protein reserves of human body.304: NUTRITIONAL AND CLINICAL BIOCHEMISTRY UNIT I 1. 4. atherosclerosis and rheumatic disorders Food allergy: Definition. Obesity related diseases and management of obesity. 2. Tissue distribution of enzymes. Clinical enzymology Use of enzymes in diagnosis. iodine. clinical features. Diagnostic significance of acid phosphates. Nutritional requirements during pregnancy. analysis. Direct and indirect calorimetry. magnesium. Role of leptin in regulation of body mass Clinical nutrition: Role of diet and nutrition in the prevention and treatment of diseases. physiological action of unavailable carbohydrates. ammonia metabolism. lactate dehydrogenase. Measurement of energy expenditure. precision. collection. iron. 3. amylase. normal and reference value determination. liver diseases and liver function test 3. Clinical applications of serum protein eletrophoresis. metabolic disorders and management of Marasmus and Kwashiorkar diseases Obesity: Definition and classification. etiology and pathogenicity. processing and preservation of specimen. Gastrointestinal tract: Disorders and diagnosis 4. ketone bodies Lipid metabolism :Diagnostic significance of analysis of serum lipids. biochemical functions. Energy requirements of man and woman and factors affecting energy requirements Dietary requirements. host and environment. renal failure. creatine kinase. 2. 3. chlolesteol and heart disease. phosphorus. sources of available and unavailable carbohydrates. Protein requirement at different stages of growth. hyperlipidemia. Hemoglobinopathies Carbohydrate metabolism : Carbohydrate intolerance. Fluorosis. Dental caries. automation and quality control Clinical significance of specific plasma/ CSF proteins. Diabetes mellitus. Lipoprotein metabolism and disorders UNIT IV 1. renal diseases and kidney function tests 2. Renal clearance. diagnosis and management of allergy UNIT III 1. lipase 23 . Thermogenic affects of foods. Essential amino acids and concept of protein quality. UNIT II 1. Protein energy malnutrition: Etiology. lactation and of infants and children 2. Basic Concepts: Composition of human body. laboratory management. Hypoglycemia. specific deficiency diseases associated with fat and water soluble vitamins. Vitamins: Dietary sources. Genetic and environmental factors leading to obesity. gamma glutamyl transfearse. types. zinc and copper. cholinesterase. Liver: Role of liver in biochemical processes. Energy contents of foods. Types of hypersensitivities. Hypervitaminosis. Bilirubin metabolism. alkaline phosphatase.

disorders and diagnosis Adrenal and gonads . 3. 4. Estimation of serum aspartate transaminase 11. 2. Electrophoretic separation of serum proteins on agarose gel 2. Disorder and biochemical assessment Practical Exercises: 1. HDL cholesterol. porphyrin and related compounds: Disorders and diagnosis Thyroid : Disorders and diagnosis Hypothalamic-pituitary-adrenocortical system: Functions. LDL cholesterol 6. Estimation of serum triglycerides 5. Tietz Text book of Clinical Chemistry 2. Estimation of serum albumin and determination of albumin : globulin ration 3. Estimation of serum total cholesterol. Electrophoretic separation of sickle hemoglobin 8. Hemoglobin. Estimation of serum acid phosphatase 9. Estimation of serum creatinine Reference Books 1.UNIT V 1. Estimation of blood glucose by glucose-oxidase method 4. Estimation of serum bilirubin 7. Clinical Chemistry by DF Calbreath 3. Estimation of serum alkaline phosphatase 10. Clinical Biochemistry by Varley Note: All text books are of latest editions 305 : Lab Course V Consists of practical exercises listed out under 301 & 302 306 : Lab Course VI Consists of practical exercises listed out under 303 & 304 24 .

2. 3. 3. 2. regeneration and somatic hybridization and applications of plant tissue culture UNIT IV 1. Nanobiotechnology. Introduction and organization of animal cell and tissue culture laboratory. 3. DNA Microarrays and Gene Expression by P. Functional proteomics: Methods pf proteome analysis Human Genome Project (HGP): The human genome/ Social implications Forensic applications of DNA analysis Patents and intellectual property: Intellectual property areas : Trademarks. 3. 4. Why obtain a patent. Ratner. 2. Daniel Ratner 25 . 4. manipulations of stem cells Factors governing manipulation of stem cell. 2. Copyrights.Hatfield Protein-Protein Interactions by Erica Golemis A passion for DNA (Genesm Genomes and Society) by J.F. Therapeutic cloning for embryoinic stem cell (ESC’s) Applications of stem cell cultures UNIT III 1. 5. 3. transfer and establishment of whole plants in soil Protoplast isolation. 2. physical and metabolic functions of different constituents of culture medium. 4. single cell clones Organogenesis : Somatic embryogenesis. 3. 2. measurement of viability and cytotoxicity Introduction to balanced salt solutions and simple growth medium: Brief introduction of the chemical. Role of carbon dioxide and supplements Biology and characterization of the cultured cells. 4. Primary and established cell line cultures and mass culture techniques Stem cell technology: Types of stem cell. Contamination.Baldi and G.D. 4.401: FRONTIER TECHNOLOGIES IN BIOSCINCES UNIT I 1. Introduction of plant tissue culture and laboratory organization Tissue culture media (composition and preparation). 4.W. Basic techniques of mammalian cell culture: Disaggregation of tissues. The process of obtaining a patent. initiation and maintenance of callus and suspension culture. Primary and established cell line culture Serum and protein free defined media and their applications. Tools for measuring nano structures Biosensor development and its applications Microarray chips: Types of DNA chips and their production SNP’s and GMS (Genome mismatch repair) UNIT V 1. culture. Nanobiotechnology: Introduction to nanoscinece. Griffiths et al.Watson Modern Genetic Analysis by Anthny J. Recent changes in IPR and Patent policies Reference Books: 1.Next Big Idea by Mark. measurement of parameters of growth UNIT II 1.

A. 26 .Brown 7.6. Gene Cloning by T. Latest information on academic Websites Note: All text books are of latest editions.

replication. Overview of Bioinformatics: Introduction to MEDLINE on PubMed System for accessing Biological Information. EST clustering. 3. MS-Excel and MS-Power Point 4. folders. randomized block design 4. Mean. Impress UNIT IV 1. Office applications: MS-Office. X. physical properties based on sequences. Test of significance. Standard error. Statistical population and sampling methods Classification ad Tabulation of data. Internal and External commands 2. folding classes. Submitting DNA sequences to database Reference Books: 1. Sequence databases: Contents. DT Jopnes and JM Thornton An Introduction to Computational Biochemistry by C. 3. Genes.402: BIOSTATISTICS. randomization. CGC. directories and their management 3. Phylips 4. Predictive methods : Predictive methods using nucleotide sequences and protein sequencing. t-test. Diagrammatic and graphical presentation Frequency distribution and measures of central value Measures of variability. chi-square test and analysis of variance 3. complementary randomized. Deviation.Sai 27 . Multiple sequence alignment programme-Clustal W. Entrez. Path analysis UNIT III 1. NCBI 2. distance matrices. tertiary structures 2. gene discovery. COMPUTERS AND BIOINFORMATICS UNIT I 1. Correlation and Regression. Parsimony. Retrieving and installing a program (Tree Tool). Concept of Hardware and Software. PIR. local control. Design of experiment. FASTA 4. Open office on Linex: Word Processor. Introduction to Biostatistics. notations and applications. Positive and negative correlation. molecular modeling 3. Sequence search UNIT V 1. Introduction to computer basics. linear regression and regression equation 2. 2. ORF prediction. 2. Structure and annotation for Human Genome Databases. 3. Concept of file. DOS. Calculation of correlation coefficient and regression coefficient. 4. Spreadsheets. Use of genome analysis programs: BLAST. Coefficient of variation UNIT II 1. Phylogenetic analysis : Phylogenetic reconstruction. Motif and Profile. Factor analysis. Range. Proteins and Computers by CA Orengo. Genome mapping applications : EST and functional genomics. MS-Word. Plant Genome Databases. Introduction to Bioinformatics: A theoretical and practical approach by Stephen a Krawetz and DD Womble Bioinformatics. secondary structures.Stan and T. Swissport. Standard deviation. Common terms.

The work is complied in the form of dissertation. JM Parish and RM Twyman Statistics for Agricultural Sciences by G Nageswara Rao Fundamentals of Statistics by Goon et al. 9. 6. Instant Notes on Bioinformatics by DR Westhead. 10. 7. _______________________________________________________________________________ 28 . 5. _______________________________________________________________________________ 403 : Lab Course VII Consists of practical exercises based on 401 & 402 _______________________________________________________________________________ 404 : Research Project Work To be carried out on a specific defined objective under the supervision of a teacher. Molecular Biotechnology : Therapeutic applications and Strategies by S Maulik and SD Patel Methods in Biostatistics by BK Mahajan Statistical Methods by SP Gupta Statistical Methods by GW Snedecor and WG Cochran Note: All text books are of latest editions. 8.4.