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Course syllabus

for

M.Sc. Virology
Conducted at the

National Institute of Virology
130/1, Pashan-Sus Road, Pashan, Pune 411 021

Under the aegis of IBB
University of Pune

36. 1. 42. 44. 20. 18. 37. 12. 7. 2. 5. 31. 17. 24. 13. 35. No. 8. 6. 33. 16. 44. Semester I VR-111(T) VR-112(T) VR-113(T) VR-114(T) VR-115(T) VR-116(T) VR-131(P) VR-132(P) VR-133(P) VR-134(P) VR-135(P) VR-136(P) Semester II VR-211(T) VR-212(T) VR-213(T) VR-214(T) VR-215(T) VR-216(T) VR-217(T) VR-218(T) VR-231(P) VR-232(P) VR-233(P) VR-234(P) VR-235(P) VR-236(P) VR-237(P) Semester III VR-311(T) VR-312(T) VR-313(T) VR-314(T) VR-315(T) VR-316(T) VR-317(T) VR-318(T) VR-331(P) VR-332(P) VR-333(P) VR-334(P) VR-335(P) VR-336(P) VR-337(P) VR-338(P) Semester IV VR-411(T) VR-431(T+P) Total credits One credit= 15 hr of interaction of students with facilitator Special topics Research Project 1 24 100 Viral Enteric Diseases & Cancers Viral Hepatitis Viral Respiratory Diseases Exanthematous Diseases of viral aetiology Viral Haemorrhagic Fevers Viral Encephalitis HIV / AIDS (Conducted at NARI) Veterinary and Agricultural Viruses Viral Enteric Diseases Viral Hepatitis Viral Respiratory Diseases Viral Exanthematous Diseases Viral Hemorrhagic Fevers Viral Encephalitis HIV / AIDS (Conducted at NARI) Veterinary and Agricultural Viruses 1 2 1 1 1 2 1 1 2 3 2 1 2 2 2 1 Gene regulation and recombinant DNA technology Viral Cell Interaction Virus Replication Advance Immunology Applied Entomology Applied Epidemiology Bioinformatics Antivirals and Vaccines Molecular techniques Biochemical/Biophysical methods Serological Methods Immunological techniques Medical Entomology Epidemiological data management and analysis Practical Bioinformatics 2 1 1 1 1 1 1 2 3 3 3 3 1 1 1 Basic Virology Tissue culture & Cell Biology Basic Immunology Basic Epidemiology & Biostatistics Vector Biology Virological Methods Analytical Methods Tissue culture techniques Virus/antigen Detection Statistical Methods Entomological Methods Propagation of viruses 1 2 2 2 1 2 2 3 3 1 3 3 Title Credits 1 . 38. 4. 25. 26. 23. 40. 34. Sc. 27. 39. 19. 30. 32. 29. Course no. 10. Virology: List of courses offered at NIV Sr. 28. 43. 14. 11. 3.M. 21. 9. 15. 22. 41.

Cell growth—hyperplasia. 2. Julian Lewis.P.who. cell division. Lawrence Zipursky. Large scale production—suspension cultures. Laboratory Biosafety Manual. containment facilities. Freeman Company. Date: September 2005. Publisher: Springer-Verlag New York. organ culture. R. Racaniello. http://www. and S. Date: August 2003. posttranslational modifications. 2 . Module 4: Cell signaling 2 hrs. Recommended Books: 1. Margi Sirois. Pathogenesis.M. protein biosynthesis. Module 5: Tissue culture methods: 7 hrs. Ian Freshney. cell adhesion and junctions. Principles of bio-safety. viruses of veterinary importance and plant viruses. J. Knipe. 4. Bruce Alberts. Howley. Latest edition / Pub.L. Signal transduction pathways. P. In vitro cultures—primary. Publisher: National Academy Press. cell types in culture. Publisher: Taylor & Francis. Chanock. growth rates. proteins. substrates. Ian Freshney. growth and differentiation factors. Jun Mitsuhashi. Philadelphia. L. 5. WHO. J. eds.Chapters 3-13. Lippincott-Raven. Wiley. Cell characterization—karyotyping. Culture of Cells for Tissue Engineering. Rancaniello. carbohydrates and lipids. Paul Matsudaira.int/csr/resources/publications/biosafety/who_cds_csr_l yo_20034/en/ VR-112(T): Tissue Culture and Cell Biology 2 Credits Module 1: Cell structure 5 hrs. Module 2: Macromolecules: 5 hrs. and differentiation—normal and transformed cells. animal and plant viruses 5 hrs. R. Skalka. Straus. R. Virus structure and morphology. S. A. 3. Latest edition / Pub. Module 3: Molecular biology 6 hrs. Date: January 1996. 3rd Edition. Cell environment—nutritional requirements. Laboratory Animal Medicine: Principles and Procedures. H. RNA. LLC. development and differentiation—cell lineages. cytoskeleton. Module 6: Developmental biology: 5 hrs.E. 2. Essential Cell Biology. D. R. Enquist. T. Flint. Inc. isoenzymes. Invertebrate Tissue Culture Methods. Date: November 2004. Guides for the Care and Use of Laboratory Animals. National Research Council. James Darnell. transcription and post-transcriptional modifications. Roizman. maintenance and handling of laboratory animals and requirements of virological laboratory. Principles of Virology: Molecular Biology. V. Stem cells --adult and embryonic. Latest edition / Pub. Module 2: Virus structures. fish and invertebrate cultures. Martin Raff. Dennis Bray. Harvey Lodish. Keith Roberts. Arnold Berk. Fields. Publisher: W. Monath.). S. Pub. etc.M. B. Latest edition / Pub. Publisher: Elsevier Health Sciences. microcarriers. diploid and established cell lines. Module 3: Infrastructure 5 hrs.Semester I: Theory courses VR-111(T): Basic Virology 1 Credit Module 1: Introduction 5 hrs. Melnick. biomembranes. hypertrophy. hollow fiber reactors. Date: December 2003 Publisher: American Society Microbiology--. History and principles of virology. V. Structure and function of cellular organelles. Molecular Cell Biology. Replication of DNA. Date: October 2003. M.M. and Control of Animal Viruses.Date: March 2006. W. 4. Fields Virology Vol 1 and 2. R. Latest edition / Pub. 5. virus taxonomy. Culture of Animal Cells: A Manual of Basic Technique. Recommended Books: 1. PA. Wiley. Structure and function of DNA. 3. Date: February 2002. B. Latest edition / Pub. Latest edition /Pub.N. introduction to replication strategies.

2. complement system. parametric and nonparametric tests. Module 4: Fundamentals of biostatistics 4 hrs. D Male & D Roth (2006): Immunology. standard deviation. GM. Kenneth J. Publisher: Elsevier Health Sciences. 7. dispersion and correlation 4 hrs. 4 hr Module 3: Antigens & Immunoglobulins: Hapten/antigen. 7. CV Mosby & Co. VR-114(T): Basic Epidemiology and Biostatistics 2 Credits Module 1: Introduction 5 hrs. Sample size estimation and introduction to study design in epidemiological investigations. 6 hr Recommended Books: 1. Elsevier. 5. Recommended Books: 1. D Roth & I Roitt (2007): Immunology: With Veterinary Consult Access. 2. RA Goldsby & BA Osborne (2007): Kuby Immunology. definitions and concepts in Epidemiology. Edn. Historical aspects and evolution of epidemiology. WH Freeman & Co. 5 hr Module 4: Antigen recognition: Major histocompatibility complex.VR-113(T): Basic Immunology 2 Credits Module 1: Introduction to immunology: Introduction and history. structure & function. Male D. 3 . Module 6: Probability and statistical inference 5 hrs. With Student Consult Online Access. Positive and negative selection. CV Mosby & Co. SJ Martin. J. Measures of central tendency. variance. M Saunders & W Tamminen (2008): Primer to the Immune Response. applications and statistical tables. J Brostoff. natural history of diseases and measures of risk and death. Cells of the immune system. 8. 3. Latest edition / Pub. J Brostoff. Publisher: Oxford University Press. 6. disease burden. Leon Gordis. Concept of significance tests. Antigen & antibody interaction. mode.Blackwell. 4 hr Module 2: Innate immunity: Innate immune response & inflammation. Human leuko-cyte antigen association with disease. 3. Module 5: Measures of location. Blackwell Publishing. England. 5 hr Module 5: Acquired immune response: Antigen presenting cells. Descriptive and analytical epidemiology. Edn. Module 2: Approaches in epidemiology 8 hrs. 11. tabular and graphical presentation of data. Kindt TJ. Module 3: Study design and sampling 4 hrs. HM. standard error and confidence intervals. Prentice Hall/ Pearson Education. 3. Ethne Barnes. Sompayrac L (2008): How the Immune System Works. 7. Primary and secondary organs of the immune system. Polymorphism. Harlow. Mean. Date: November 2004. Immunoglobulin classes. Rothman. 4. coefficient of variation. Edn. With Student Consult Online Access. Wood P (2006): Understanding Immunology. types of data. Latest edition / Pub. Delves PJ. quartiles Measures of dispersion—range. Publisher: University of New Mexico Press. Cytokines & Chemokines. DR Burton & IM Roitt (2006): Roitt’s Essential Immunology. Antibody diversity. Epidemiology. Normal distribution—density curves. Edn. Date: May 2002. Co-stimulation. 6 hr Module 6: Assignments/ Presentations: These will be based on the above 5 modules. Edn. 6. Abbas AK & AH Lichtman (2006): Basic Immunology: Functions and Disorders of the Immune System. Latest edition / Latest edition / Pub. WB Saunders Co. Date: March 2005. antibody. Edn. T and B cell stimulation. Ontology. Concept and probability distribution. Mak TW. Introduction. median. Wiley. Roitt I. Epidemiology: An Introduction. Diseases and Human Evolution. 2.

Trapp. Evelyne T. Methods for collecting these insects and arthropods. Latest edition / Pub. Beth Dawson. Recommended Books: 1. Date: July 1993. Edwin H. their behavior and ecology with special reference to dengue. Stephenson (Editor). Andy Field. their preservation/ maintenance and transportation. Trudel (Editor). Introduction to general entomology. F. Latest edition / Pub. Culex and Anopheles. ecology. Publisher: Humana Press. 4. Recommended Books: 1. Immnuodiagnosis. estimation of yields. Ticks and mites 5 hrs. 2. Date: November 2003. collection and preservation 5 hrs. Mahy (Editor). 5. Biology and morphology of fleas.principles and applications. methods for purification of viruses with special emphasis on ultracentrifugation methods. Electrophoresis. 4 . John R. 6. Hillar O. Module 3b) Microscopic techniques : 3 hrs. Evelyne T. chromatography. Lennette (Editor). Bates M (1949) Natural History of mosquitoes The Macmillan Co Baker RH and Wharton R(1952) Introduction to Acarology The Macmillan Co VR-116(T): Virological methods 2 Credits Module 1: Cultivation and purification of viruses 5 hrs. neutralization. Date: August 1998. Lavoipierre MMJ (1962) Entomology for students of Medicine. insect morphology and classification Insects and other arthropods of medical importance. and their structures and functions. Service MW (1996) Medical entomology for students. Rickettsial. Kangro (Editor). Robert Trapp. Biology and life history of Aedes. Biology. In vivo. Javier Nieto. Chapman and Hall Kettle DS (1984) Medical and veterinary entomology CAB international Richard and Davies Imm’s general Text book of Entomology. and Chlamydial Infections. Fluorescence. Diagnostic Virology Protocols: Methods in Molecular Medicine. culicoides. Latest edition / Pub.J. Publisher: Marcel Dekker. life history of ticks with special reference to Kyasanur Forest Disease (KFD). Latest edition / Pub. Gordon RM. X-ray crystallography. flowcytometry and imunohistochemistry. Publisher: Jones & Bartlett Publishers. Inc. Date: January 1996. Lennette (Editor). Western blot. Biology and morphology of mites. 4. chikungunya. Japanese encephalitis and West Nile Module 3: Biology and ecology of other blood sucking insects. Publisher: SAGE Publications. microarray and nucleotide sequencing. Robert G. 5. David A. Vol I & II. Nucleic acid hybridization. Complement fixation. 3. Methods and Techniques in Virology. Latest edition / Pub. Epidemiology: Beyond the Basics. Module 3a) Nucleic acid based diagnosis 7 hrs. membrane filtration. Discovering Statistics Using SPSS. haemagglutination and haemagglutination-inhibition tests. RIPA. in vitro and in ovo systems for virus growth. Diagnostic Procedures for Viral. Blackwell Scientific Publ. Brian W. Basic and Clinical Biostatistics. Lennette. polymerase chain reaction. Moyses Szklo. 2.4. Virology Methods Manual. Date: January 1995. Module 2: Diagnostic methods 10 hrs. NMR. Alan Warnes Latest edition / Pub. 3. 7. 6. Publisher: American Public Health Association Publications. Publisher: Elsevier Science & Technology Books. lice. Date: March 2004. Latest edition / Pub. Pierre Payment.) Lennette. Biology. (Eds. Chapman and Hall Roy DN and Brown AWA (1970) Entomology (Medical & veterinary) Bangalore printing and Publishing co. confocal and electron microscopic techniques -. VR-115(T): Vector Biology 1 Credit Module 1: Insect morphology. Date: April 2005. Module 2: Biology and ecology of mosquitoes 5 hrs. morphology and disease relationship of sandflies (sandfly fever and chandipura). Module 4: Analytical techniques 5 hrs.

hrs. hrs. hrs. hrs. hrs. hrs. hrs. hrs. Mosquito collection & taxonomy Taxonomy of ticks and sandflies Processing of arthropods Mosquito inoculation & immunoflourescence Insecticide testing Collection of rodents hrs. Protein estimation (Lowry) DNA estimation (colorimetric and spectrophotometric) Gel filtration chromatography Polyacrylamide gel electrophoresis Confocal microscopy 3 Credits 5 hrs. 5. 2 Credits 5 8 5 8 4 hrs. hrs. 6. sterility checks Maintenance of cell cultures Preparation of primary cell culture (CEC) 3 Credits VR-133(P): Virus / Antigen detection 1. 16 hrs. 6. 4. 6. 5. Routes of inoculations in embryonated eggs 18 hrs. 5. hrs. 2. 3.plaque assay & TCID50 2. 5 . hrs. VR-134(P): Statistical Methods 1. 2. 3. Glassware decontamination. Estimation of virus yields-. hrs. 2. 4. hrs. VR-136(P): Propagation of viruses 1. VR-135(P): Entomological methods 1. hrs. hrs. 4. 2. 3. 3. VR-132(P): Tissue culture techniques 1. packing and sterile handling Media and reagents preparation. 4. hrs. Graphical presentation of data Measures of central tendency Correlation and regression analysis Significance tests Statistical packages Epidemiological exercise hrs. 2. hrs. hrs. ELISA Immunoflourescence assay Heamagglutination Agar gel diffusion Polymerase chain reaction Electron microscopy 8 8 8 5 8 8 1 Credits 3 3 3 3 3 3 3 Credits 8 8 8 8 8 5 3 Credits hrs. 3. sterilization. 8 hrs. washing. 9 hrs. 18 hrs. 5. hrs.Semester I: Practical Courses VR-131(P): Analytical methods 1. 4. Preparation virus stocks and determination of mouse LD50 3. 16 hrs.

Pathogenesis. cosmids. Martin A. Viral vectors—retroviral. Robert May. Recommended Books: 1. LCM and HIV). Module 2: Eukaryotic gene expression 5 hrs. J. Stow (Editor) Latest edition / Pub. 2. endocytosis and virus uncoating mechanisms Nuclear localization signals and nuclear pore transit. R. apoptosis. Rancaniello. control of transcription initiation and termination. 3. chaperons. Date: July 2002. Racaniello. Bell. W. stress response. L. Nowak. Molecular Biotechnology: Principles and Applications of Recombinant DNA. David Bowtell (Editor). Date: December 2003 Publisher: American Society Microbiology. Baker Latest edition / Pub. Michael B. Chromosomes. Molecular Biology of the Gene. VR-212(T): Virus-cell Interaction 1 Credit Module 1: Cellular receptors and virus entry 5 hrs. VSV. Glick. John & Sons. pox. Watson. 6 . Latest edition / Pub. Richard Losick. Phage display libraries. Molecular Aspects of Host-Pathogen Interactions. ultrastructural cytopathology. Cellular injury associated markers.Latest edition / Pub. M. Publisher: Wiley. Eukaryotic vectors—yeast. James D. and Pathogenesis. V. mechanism of viral persistence and latency—in vivo and in vitro models (JE. Richard W. 4. Date: September 2002. measles. Module 3: Cloning vectors 5 hrs. and Control of Animal Viruses. Flint. Saunders (Editor). Compans. Cellular interactions—clathrin coated pits. BAC and YAC Module 4: Expression vectors 10 hrs. Module 2: Virus morphogenesis 3 hrs. Date: September 1997. 4. Prokaryotic. reverse genetics. Publisher: Cold Spring Harbor Laboratory Press. rhabdo and adeno virus vectors. Enquist. Smyth (Editor). IRES. structure and methods of discovery of viral receptors (polio. A. N. Latest edition / Pub. Joseph Sambrook (Editor). M13 phage. regulatory elements. Recommended Books: 1. splicing and RNA processing. Genes VIII. Cell Biology of Virus Entry. S. Latest edition / Pub. Michael Levine. J. Latest edition / Pub. D. Plasmids. R. alteration of signaling pathways. Bernard R. lipid rafts. Ari Helenius (Editor). J. Date: December 2003. Skalka Latest edition / Pub. cellular basis of transformation. viral replicons (SFV and HCV). Incorporated. Oldstone (Editor). Malcolm A. Replication sites and their characterization. transport of viral proteins. Publisher: Prentice Hall. 2. Definition. Module 3: Mechanism of host cell damage 3 hrs. Replication. caveolae. Date: December 1988. mammalian and insect cell systems. chromatin structure. 3. lambda phage. McCrae (Editor). Fusion proteins—signals for protein secretion. Module 5: Novel strategies 5 hrs. Publisher: Cambridge University Press. Publisher: Oxford University Press. purification of recombinant proteins. C. replicones. R.Semester II: Theory courses VR-211(T): Gene Regulation & Recombinant DNA technology 2 Credits Module 1: Prokaryotic gene expression 5 hrs. R. Polymerase-promoter interactions. Kinetics of receptor binding. Principles of Virology: Molecular Biology. Jack J. Virus Dynamics: Mathematical Principles of Immunology and Virology. Benjamin Lewin. V. Host cell ‘shut off’. Pasternak. Publisher: ASM Press. herpes. Date: January 2000. DNA Microarrays: A Molecular Cloning Manual. virus –cytoskeletal interactions. HIV). Module 4: Cellular gene expression 4 hrs. types of cenotaphic effects. necrosis. Date: December 2003 Publisher: Benjamin Cummings.

2 hrs. Replication of double stranded RNA virus (rota). 4. Racaniello. DNA Virus Replication. negative strand RNA viruses (VSV and influenza). Lippincott/ Williams & Wilkins. 3 hr Module 2: Effector Mechanisms: Phagocytosis. Module 3: DNA viruses 3 hrs. 3. 3 hr Module 3: Immunoregulation: Antigen-antibody. Publisher: Elsevier Science & Technology Books. Complement mediated regulation. Garland Science. Prion proteins. W. 6 hr Module 4: Assignments: These will be based on the above three modules. Edn. 4. replication of plant virus (Poty). Date: June 2005. Transplantation immunology. Flint. Recommended Books: 1. L. S. V. pox). Alan Cann J. Date: March 2000. 2. Publisher: Oxford University Press. Module 2: Other RNA viruses 5 hrs. Principles of Virology: Molecular Biology. and Control of Animal Viruses. WH Freeman & Co. 7. Kindt TJ. Alan J. Edn. 3. ambisense RNA (LCM) and retroviruses (HIV and HTLV). R. Mucosal effector mechanisms. 1 and 2. Elsevier Health Sciences. ssDNA virus (AAV) Module 4: Miscellaneous. R.VR-213(T): Virus Replication 1 Credit Module 1: RNA viruses: 5 hrs. Principles of Molecular Virology. 3 hr Recommended Books: 1. Natural killer and gamma delta cells. J. A. Vol. V. Latest edition / Pub. Cann. Rancaniello. General strategies. Murphy K. Pathogenesis. P Travers & M Walport (2007): Janeway’s Immunobiology. cytokine-mediated immune regulation. Field Virology. Johnson AG (2005): High-yield Immunology. New York. Cytotoxic and T helper response. replication of plus stranded RNA virus (polio). Hypersensitivity. Abbas AK & AH Lichtman (2005): Cellular & Molecular Immunology. VR-214(T): Advanced Immunology 1 Credits Module 1: Mucosal Immunity: Cells and organs of the mucosal immune system. Cann.. Enquist. 2. M. immunodeficiency. Latest edition / Pub. Replication of double stranded DNA viruses (SV40. Skalka Latest edition / Pub. RA Goldsby & BA Osborne (2007): Kuby Immunology. Date: December 2003 Publisher: American Society Microbiology. Autoimmunity. 6. 7 .

Vol I & II. Epidemiology: Beyond the Basics. 7. host preferences of vectors and their influence. Diseases and Human Evolution. genetics of populations.VR-215(T): Applied Entomology 1 Credit Module 1: Vector virus relationship 3 hrs. interfere with arbovirus infections. 2. Moyses Szklo. Module 3: Vector Control 5 hrs. natural cycles and population biology of vector borne pathogens. Influence of man in natural focality. Chapman and Hall. Rothman. Leon Gordis. Publisher: Oxford University Press. Kettle DS (1984) Medical and veterinary entomology CAB international. Latest edition / Pub. Control in urban settings Control at aquatic stages. Bates M (1949) Natural History of mosquitoes The Macmillan Co. evolutionary Genetics] Applied Genetics [Breeding systems. possible usage of transgenic mosquitoes. molecular approach to Taxonomy. Javier Nieto. Andy Field. Baker RH and Wharton R(1952) Introduction to Acarology The Macmillan Co. Discovering Statistics Using SPSS. Gordon RM. Latest edition / Pub. Case control and cohort studies. Latest edition / Pub. Needs and steps to be taken for outbreak investigations. Richard and Davies Imm’s general Text book of Entomology. mechanical transmission. Module 2: Analytical epidemiology 4 hrs. Ethne Barnes. Module 2: Epizootiology of vector borne viral diseases 2 hrs. Beth Dawson. J. Epidemiology: An Introduction. spatial structure and geographic variations. Mosquito Genetics: Basic Genetics--mutants of special interest. Latest edition / Pub. adult population. Date: March 2005. natural cycle. GIS in vector borne viral diseases. 3. 2. insecticide resistance mechanism and control dynamics.methods and application. Blackwell Scientific Publ. Date: April 2005. proteins as Taxonomic markers. personal protection. Publisher: Jones & Bartlett Publishers. Various control strategies and environmental management. Kenneth J. Inc. Genetic control] Transgenic vectors: Transgenic mosquitoes. 5. 8 . Recommended Books: 1. Module 3: Outbreak investigations 6 hrs. Roy DN and Brown AWA (1970) Entomology (Medical & veterinary) Bangalore printing and Publishing co. ecological aspects. 3. Publisher: Elsevier Health Sciences. Latest edition / Pub. Molecular Characterization of vectors: Species complexes. Animal movements. Trapp. F. Lavoipierre MMJ (1962) Entomology for students of Medicine.intrinsic and extrinsic factors. Robert G. 4. basis of vector competence. susceptibility-. Xenodiagnosis. biochemical and molecular Taxonomy for detection of intra -species variation. Date: November 2004. Formation of natural foci of diseases. Service MW (1996) Medical entomology for students. Epidemiology. Latest edition / Pub. genetic manipulation. Chapman and Hall. 6. Recommended Books: 1. collaboration with State and national health authorities. Publisher: SAGE Publications. Module 4: Molecular Entomology 5 hrs. Types and methods of public health and infectious disease surveillance. Basic and Clinical Biostatistics. Virus dissemination & mechanism of virus transmission in vectors. Publisher: University of New Mexico Press. 5. chromosomal variants. 4. Date: May 2002. 6. establishing surveillance system. virus dissemination. Date: March 2004. Robert Trapp. Date: November 2003. VR-216(T): Applied epidemiology 1 Credit Module 1: Public health surveillance 5 hrs. maintenance of viruses in nature.

visualization tools. Plotkin. DNA vaccines. Latest edition / Pub. Vaccines. Rainer Ulrich (Editor). Introduction to Bioinformatics--. epitope prediction. FASTA. ribozymes. subunits. Module 2: Antivirals 10 hrs. database similarity searches—BLAST. sequence data forms and submission tools.Attwood. Instant notes in Bioinformatics---Westhead. John & Sons. 4. antigen-antibody interactions. mechanisms of action. Stephen K. Recommended Books: 1. structure evaluation and validation. Conventional vaccines -killed and attenuated. search engines. proteins. and Immunotherapies. homology based methods for protein tertiary structure prediction. genomes—structure data bases. in silico approaches for drug designing. S. 3. Date: July 2005. Walter A. Vaccines. Introduction to Bioinformatics---Lesk. Module 3: Modern approaches of virus control 5 hrs. immunomodulators (cytokines). scoring matrices for sequence alignments. phylogenetic analysis and tree building methods. large scale manufacturing—QA/QC issues. Incorporated. Nucleic acid. Interferons. 3. siRNA. Inc. 4.VR-217(T): Bioinformatics 1 Credit Module 1: Introduction and biological data bases 4 hrs. Qoellette. threading approaches. VR-218(T): Antivirals and Viral Vaccines 2 Credits Module 1: Viral Vaccines 10 hrs. motif searches. peptides. Publisher: Marcel Dekker. Stanley A. Orenstein. A. Module 2: Methods for sequence analysis 6 hrs. modern vaccines—recombinant proteins. Publisher: Elsevier Health Sciences. Protein secondary structure prediction. promoter and gene prediction. Module 3: Structure based approaches 5 hrs. Publisher: Wiley. designing and screening for antivirals. Multiple sequence alignment. algorithms—pairwise sequence alignments. Detlev H. Bioinformatics: A practical guide to the analysis of genes and proteins—Baxevanis. Antiviral Drug Discovery for Emerging Diseases and Bioterrorism Threats. antiviral libraries. Date: November 1996 Publisher: Karger. Chimeric Virus -like Particles as Vacc ines. data mining tools and applications. 9 . comparative analysis. NY. Latest edition / Pub. Paul F. Antiviral Agents. Latest edition / Pub. Latest edition / Pub. antiretrovirals—mechanism of action and drug resistance. Date: September 2003. group discussions and presentations 5 hrs. Tyring. vaccine delivery and adjuvants. 2. 2. Wolfram H. Parish & Twyman. Krueger (Editor). Date: October 2004. John Wiley & Sons. Module 4: Assignments. Recommended Books: 1. Anti-sense RNA. Gerlich (Editor). Torrence (Editor).

VR-237(P): Practical Bioinformatics 1. Ultracertifugation VR-233(P): Serological methods 1. MS Excel 2000 4 hrs. Western Blot 5. hrs. Mosquito inoculation and IFA 2. 4. Plaque reduction neutralization test 4 Credits 1 2hrs. hrs. 2. hrs. 12 hrs. Restriction endonuclease digestion 5. hrs. Hybridoma (fusion and limiting dilution). 3. 4 hrs. 3. Ultrafitration 6. Molecular modeling & visualization 3 3 3 3 3 hrs. DNA and RT-PCR VR-232(P): Biochemical/ Biophysical methods 1. 5. Epitope prediction 5. Complement Fixation test 4. Polyacrylamide gel electrophoresis 4. Purification of plasmid 4. 12 hrs. 8 hrs. 8 hrs. Organs of the immune system (from mouse). Assay for the separation of B and T cells. hrs. Cytokine assay. 3Credits 3 Credits VR-234(P): Immunological techniques 3 Credits 1. Elispot assay. Biological data banks 2. 8 hrs. 1 Credit 10 . 7. Assay for antigen presentation by phagocytosis. Pairwise sequence alignment 3. Bird. 12 hrs. 8 hrs. 10 hrs. Separation of adherent and non-adherent cells (both from splenocytes and PBMCs). Plasmid transformation 3. VR-235(P): Medical entomology 1. Native PAGE and isoenzyme analysis. Statistical softwares 7 hrs. 8 hrs. Insecticide (larval & adult) bioassays 2 Credits 8 4 4 6 8 hrs. 16 hrs. Protein estimation 3. MS Access 2000 4 hrs. IgM capture ELISA 3. hrs. Phylogeny & tree building 4. Bat trapping 3. Isolation of PBMCs by various methods. hrs. Rodents. Heamagglutination inhibition test 2. 12 hrs. 2. 9 hrs. 6. 1Credit VR 236(P): Epidemiological data management and analysis 1. Flowcytometry. 5. Lymphocyte proliferation assay. 8. Dissection of mosquitoes 4. Growth & Preparation of competent cells 2. Motif data bases. Protein A Affinity chromatography 2. 12 hrs. 10.Semester II: Practical Courses VR-231(P): Molecular techniques 1. 9. hrs.

2001. Elsevier Perspectives In Medical Virology. Viral Infections of the Gastrointestinal Tract. Uk Isa K. By Isa Mushahwar. Second Edition (Richmans Hayden). HIV. immunopathogenesis and vaccines under development. Viral Hepatitis Molecular Biology Diagnosis and Control. prevention. Elsevier Perspectives In Medical Virology. 2nd ed. Module-2: Viral Cancers 7 hrs. Vol. Zuckerman . clinical features and differential diagnosis. 2002. Vol.A. Historical aspects. epidemiology. Uk Isa K. Viruses and Liver Cancer. 34. Structure & genomic organization. 8.Semester III: Theory courses VR-311(T): Viral Enteric Diseases and Cancers 1 credit Module-1: Perspectives of Viral Diarrhoea: 8 hrs. Human Papilloma Viruses. Latest edition / Pub. and expanded. 2002. HCV & HEV. Tabor. Hepatitis Viruses (Japan medical research fourm). Albert Z. Date: June 2001. rev.J. 28. J. Mushahwar. A. Elsevier Perspectives In Medical Virology. Harley A. genotypes. 6. and Peter M Howley Chapters: 24. Edited By U. HBV. Module 2: Structure & genomic organization 7 hrs. diagnosis. 3. Gray.7 hrs. Zuckerman . serotypes of HAV. 67 and 68. Physiology of Jaundice. 10.J. Grand. Recommended books: 1. Z. Zuckerman . 4. Module 5: Prevention & therapeutic approaches 6 hrs.. Vol 2 Ed by David M Knipe. Viral Gastroenteritis. Series Editor: Arie J. Other viruses associated with diarrhoea and gastroenteritis: Adenoviruses. Elsevier Perspectives In Medical Virology. Kapikian A. Uk Isa K.. Rotavirus diversity. VR-312(T): Viral Hepatitis 2 credits Module 1: Clinical presentation and epidemiology of viral hepatitis. Volume 2. 4. disease burden. 5. astroviruses. 1995. 54. McCance. Therapeutic possibilities of the present and future. Mutations in hepatitis viruses. 238. Rotbart (Editor). 55. prevention. Edited by E. Series Editor: Arie J. Mushahwar. presentations of hepatitis caused by different hepatitis viruses. 2003. Cell Transformation. 2. Mary Estes. risk factors. Series Editor: Arie J. Zuckerman . 3. Edited by D. vaccines presently used & vaccines of the future. Module 3:Diagnostics 6 hrs. Zuckerman . Viral Hepatitis and Liver disease. Series Editor:Arie J. Latest edition / Pub. Elsevier Perspectives In Medical Virology. Recommended books: 1. Gastroenteritis Viruses. Series Editor: Arie J. and treatment. Novartis Foundation Symposium. Module 4: Immunopathogenisis & animal models 4 hrs. Edited by J. and Cancer. Role of papilloma. Clinical course. 2. emerging strains. types of hepatitis vaccines.(2001). Animal models and their uses. Clinical Virology. Epstein Barr Virus. 7. 5. Mushahwar. Desselberger. Viruses. 6. Kapikian. Fields Virology. Mushahwar. replication. Viral Infection of Humans (S. Uk Isa K. Norwalk and Sapporo-like viruses and Enteroviruses Other enteroviral diseases. 11 . Mushahwar. 4th Ed. Serological and molecular diagnosis of different hepatitis viruses.2003. Zuckerman. American Society Microbiology. Date: March 1994. Immunopathogenisis of different hepatitis viruses. Human Enterovirus Infections. HTLV and herpes in pathogenesis of cancers. 4th edition:. Uk Isa K. January. Vaccination as preventive measure in public health. Fields Virology. Svans & A Kaslow).

. Immunization Safety Review: Vaccines and Autism Immunization Safety Review Committee (Editor) The National Academies Press. Biology and pathogensis of SARS. CRS. Clinical features. Robert Silverstein. . Recommended books: 1. of influenza. Common features of viral pox diseases and case definitions. ASM Press. Module 3:Pox diseases 3 hrs. 3 hrs. RL Hodinka. 3. eradication and control programs. Zuckerman . 2. 2.case definition and risk factors. Uk Isa K. July 1997. Pathogenesis of disease. Alicia R. Differential diagnosis of different respiratory diseases. Edited by C. Gable. Paraspecific immunity due to pox vaccination. History. SA Young. Krugman’s Infectious Diseases of children By Saul Krugman. Silverstein. Padma Shetty. USA. case definition and associated risk factor. June 2001. Potter. Preventive and therapeutic modalities. Metapneumovirus. strategies for prevention and treatment. clinical features. Recomended books: 1. Virginia Silverstein. Mushahwar. biology and immunopathogenesis. Stratton. Module 2: Rubella. Influenza. RSV and other respiratory diseases. 3. Immunization Safety Review: Measles-Mumps-Rubella Vaccine and Autism. 4.Clinical aspects of Parvovirus B –19. Mercel Dekker. epidemiology. 12 . disease burden. Vaccines against different viral respiratory diseases. Elsevier Perspectives In Medical Virology. mumps and Poxviruses 7 hrs. 2002. Module 2: Biology of respiratory viruses.W. Module 3: Diagnostics 3 hrs. Clinical features. Module4: Vaccines 4 hrs. Kathleen R. Viral Infections of Respiratory Tract by Raphael Dolin and Peter Wright. disease burden of Rubella. Series Editor:Arie J. VR-314(T): Viral Exanthematous Diseases 1 credit Module1 : Measles and SSPE 5 hrs. human rhino virus and Corona virus etc. Clinical Virology Manual Ed: Specter. Virginia B. Alvin Silverstein.VR-313(T): Viral Respiratory Diseases 1 credit Module 1: Origin and evolution of viral respiratory diseases 5 hrs. CRS and mumps. Measles and Rubella.

4. treatment Module4 Pathogenesis 7 hrs. John Booss (Editor). Viral Encephalitis. Date: June 2003. Mumps encephalitis. other rhabdoviral neurotropic agents. Module 3: Haemorrhagic manifestations caused by other viruses 6 hrs. basic principles. Dengue and Dengue Hemorrhagic Fever. Date: January 1998. Date: January 2004. KFD. Latest edition / Pub. endemic areas. VR316(T): Viral Encephalitis 2 Credits Module 1 Overview: 7 hrs. CRC Handbook of Viral and Rickettsial Hemorrhagic Feverby James H. Marburg and Ebola. factors affecting the neurovirulence. Elsevier. spread of the outbreak in relation to causative agent Laboratory diagnosis of viral encephalitic agents. role of non human hosts. Prevention and treatment of Yellow Fever. disease burden. Module 3 Other viruses 8 hrs. Virus replication strategy. Mushahwar. G. choice of laboratory diagnostic tests and their interpretation for differential diagnosis. Prevention and treatment of Dengue Role of humoral and cell mediated immunity and viral factors in development of DHF. Rift Valley Fever. Publisher: Cozy Graphics Corporation. seasonality. Hanta. spread of the disease. Module 2 JE. seasonality. encephalopathy and meningitis clinical symptoms and causative agents. Japanese encephalitis and West Nile viral infections. By C. Series Editor:Arie J. S. seasonality. and Rickettsial fevers Development of killed KFD vaccine. Margaret Esiri (Editor). Recommended books: 1. differential diagnosis. Recommended books: 1. Clinical samples required. Uk Isa K. 2. Latest edition / Pub. Module2:Dengue and DHF 6 hrs. 13 . Encephalitis caused by alpha viruses Encephalitis caused by Nipah and hendra virus. Zuckerman . History and Disease burden. Latest edition / Pub. Pathogenesis. differential diagnosis of DF and DHF on the basis of clinical symptoms. Viral Encephalitis in Humans. endemic areas. Chikungunya. Esin. Gear. Encephalitis Protection. role of non human hosts. Causative agents. blood brain barrier. 3. association with immunosuppression. Transmission. Gubler (Editor). 2004. diagnosis in sporadic cases. Risk factors and geographical distribution of viruses associated with haemorrhagic fevers and their impact on global health. genotypes vaccines Chandipura encephalitis. Qingshan Liang. Pathogenesis. Common clinical features of Viral Haemorrhagic Fevers. Encephalitis/ encephalopathy caused by measles virus. treatment modalities. preferred methods and problems.VR-315(T): Viral Haemorrhagic Fevers 1 credit Module1:Clinical course of viral infections 3 hrs.R. J. Routes and modalities of infections of the nervous tissue. Virus replication strategy. WN CHP 8 hrs. Margaret M. Publisher: ASM Press. Howard. disease burden. Enteroviral encephalitis and meningitis. reactivation vs primary infections. D. Herpes virus encephalitis. Kuno (Editor). Bioterrorism Hemorrhagic Viruses Manual: For Healthcare Workers and Public Latest edition / Pub. Animal models and vaccine potency testing. Perspectives In Medical Virology. Date: April 2004. Viral Haemorrhagic Fevers. genotypes. 2.

authors: Frank Fenner et al. Viral diseases of horticultural crops. Nairobi sheep disease/ Ganjam. characterization and viral estimation. 2. Dog diseases: Rabies. USA. Avian influenza. Disease burden. Epidemiology and risk factors. Structure and replication of HIV. Recommended books: 1. Hog cholera/ swine fever Horse diseases: Equine influenza. Module 3:Plant viral diseases 4 hrs. Infectious canine hepatitis. API Textbook. Veterinary Virology. Social and behavioural aspects of prevention and control. California. Merigan. Equine infectious anemia and equine encephalitis. Latest edition / Pub. 14 . 2006. Recommended books: 1. Vetrinary Medicine by Blood and Henderson. Dani Bolognesi (Editor). laboratory diagnosis of HIV infection. Cattle diseases: Foot and Mouth Disease. Global epidemiology of HIV. virus replication strategy. Date: September 1998 . Viral diseas es of honeybees. 3. Saag (Editor). HIV isolation. Aids Therapy. Antiviral therapy and drug resistance HIV vaccines. Henry Masur (Editor). Module:3 Preventive and therapeutic approaches 2 hrs. Royal Society of Medicine Press Ltd. Rinderpest. immunopathogenesis of infection. Module 4: origin of HIV. Bovine Spongiform encephalopathy Sheep and goat diseases: Bluetoungue. silkworm and fishes. Clinical presentation and diagnosis. Viral diseases of laboratory animals. 2. epidemiology of HIV in India. Viral insecticides. Raphael Dolin. Viral diseases of agricultural crops. Viral diseases of forest plants. Harvey J. Michael S. Module:2 Biology of HIV and its detection 5 hrs. Poultry diseases: Newcastle disease. SIV 3 hrs. HIV and Aids by Michael A. Latest edition / Pub. Date: March 2005 Publisher: Benjamin Cummings. Trials pertaining to prevention and therapy. David Wessner. 5. VR0-318(T): Veterinary and Agricultural viruses 1 credit Viral diseases of veterinary importance will cover History. II edition. Palladino. Module1:Farm animals 6 hrs. HIV -2. Publisher: Lippincott Williams & Wilkins. Marek’s disease. Thomas C. Chapter by DA Gadkari. Canine distemper Module 2:Poultry and other animals 5 hrs.VR-317(T): HIV/ AIDS 1 credit Module:1 Natural History of AIDS 5 hrs. Textbook of Aids Medicine. Bovine Ephemeral fever. John G. opportunistic infections in HIV infected individuals. Latest edition / Pub. Rift Valley Fever Pig diseases: Swine influenza. Peste des Pestits ruminants. Date: November 2002. HIV Libman. zoonotic importance and Prevention and treatment of species of agricultural importance.Natural history. Bartlett (Editor). Date: November 2002. Japanese Encephalitis. Makadon. Latest edition / Pub. 4. Pathogenesis. Academic press. Inc. Sexually transmitted diseases and their relation with HIV.

Serum ALT. hrs. hrs. Sample processing and ELISA 3. agarose gel electrophoresis interpretation 3. 5 hrs. Sample collection 2.Semester III: Practical Courses VR-331(P): Viral Enteric Diseases 1. hrs. 10 hrs. hrs. MAC-ELISA. 10 hrs. 1 Credit 15 . 8 hrs. hrs. Mouse inoculation and observation of sickness 4. Neutralization Test 5. hrs. Measles (IgG. 10 hrs. IgM) diagnosis 2. Bile pigments 2. 2 Credits 10 hrs. 10 hrs. HI test VR-334(P): Viral Exanthematous Diseases 1. Diagnosis of Chandipura virus infections 5. 10 hrs. MAb based serotyping of rotativiruses 6. Antigen capture ELISA and Immunofluorescence VR-337(P): HIV / AIDS 1. 2 Credits 5 5 5 5 5 5 hrs. Antigen detection systems 6. 10 hrs. Measles PCR VR-335(P): Viral Haemorrhagic Fevers 1. Pre-Core mutant analysis VR-333(P): Viral Respiratory Diseases 1. Virus isolation 5. hrs. Sample processing for virus isolation and IFA 3. hrs. Flavivirus neutralization tests for differential diagnosis 2. Haemagluttination inhibition assay VR-336(P): Viral Encephalitis 1. hrs. CD8 counts VR-338(P): Veterinary and Agricultural viruses Syllabus yet to be framed. 2 Credits 5 5 5 5 5 5 hrs. hrs. Sample collection and documentation of case reporting form’ 2. RNA extraction by Trizol method. RNA PAGE 4. 2 Credits 12 hrs. PCR. hrs. Multiplex RT-PCR for serotyping. hrs. HBV DNA PCR (DNAzol / Column method) 3. 2 Credits 5 5 5 5 5 5 hrs. hrs. 5 hrs. RT_PCR VR-332(P): Viral Hepatitis 1. IgM) diagnosis 3. HIV Diagnosis 2. HA test 6. HIV subtyping 3. RT PCR of JE and WN viruses 3. HAV RNA PCR (TRIzol / Column method) 4. 1 Credit 5 hrs. Urine Bile salt. Reverse transcription 2. CD4. 3 Credits 5 hrs. hrs. Real Time PCR quantitation for HBV DNA 5. Rubella (IgG. hrs. IFA 4. 10 hrs.

Ethical issues in biotechnology 7. Trade Related Intellectual Property Rights (TRIPS) and public health 11. How to write a research proposal 2. Patenting in biotechnology 10. Other topics on regulatory issues VR-431(T+P) Research Project 24 credits 1Credit 16 .Semester IV List of courses VR-411(T): Special topics List of special topics 1. Role of laboratories in virological studies 4. How to write a scientific paper 3. Indian patenting system 9. Ethical and regulatory issue in animal experiment 6. Ethics in Biomedical Research 5. Basics of Intellectual Property Rights 8.