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STUDY GUIDE & PRACTICE QUESTIONS

STUDY GUIDE & PRACTICE QUESTIONS REVISED WINTER 2012

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TABLE OF CONTENTS
INTRODUCTION ..................................................................................................................................... 4 1. LAB RULES & BASIC LAB PRACTICES .................................................................................................. 5
BASIC LAB PRACTICES ..................................................................................................................................... 5 LABORATORY REGULATIONS UCSD Teaching Laboratories ............................................................................. 9 Prepare & Protect Yourself ............................................................................................................................. 9 Prevent Accidents and Spills ..........................................................................................................................10 Respond Appropriately to Accident, Spill, or Sudden Illness ..........................................................................11 LAB RULES & BASIC LAB PRACTICES Questions: .............................................................................................12

2. FIRE SAFETY ..................................................................................................................................... 13
FIRE PREVENTION..........................................................................................................................................13 FIRE RESPONSE..............................................................................................................................................14 FIRE SAFETY Questions: .................................................................................................................................15

3. EMERGENCY & DISASTER RESPONSE................................................................................................ 16
PREPARE TO RESPOND ..................................................................................................................................16 Prevent Injuries and Spills .............................................................................................................................16 CLOTHING FIRE: STOP! – DROP! – ROLL! .......................................................................................................17 FIRST AID ......................................................................................................................................................17 MINOR INJURIES: BURNS & CUTS .................................................................................................................18 SUDDEN MAJOR ILLNESS ...............................................................................................................................18 HAZARDOUS CHEMICAL SPILL ON SKIN OR IN EYE .........................................................................................18 BUILDING EVACUATION ................................................................................................................................19 EMERGENCY RESPONSE INFORMATION ........................................................................................................20 BUILDING EVACUATION Natural Science Building ..........................................................................................21 BUILDING EVACUATION York Hall .................................................................................................................22 EMERGENCY AND DISASTER RESPONSE Questions: .......................................................................................23

4. HAZARDOUS MATERIALS ................................................................................................................. 24
UNDERSTAND THE HAZARD – THEN MINIMIZE THE RISK ...............................................................................24 HAZARD IDENTIFICATION ..............................................................................................................................25 HAZARD ASSESSMENT ...................................................................................................................................27 HANDLING & STORAGE .................................................................................................................................29 HAZARDOUS MATERIALS Questions: .............................................................................................................31

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5. HAZARD COMMUNICATION ............................................................................................................. 33
MSDS ORGANIZATION ...................................................................................................................................34 LOCATE AN MSDS & BUILD YOUR COLLECTION ..............................................................................................35 LABEL YOUR MATERIALS ...............................................................................................................................35 Other sources of information ........................................................................................................................36 HAZARD COMMUNICATION Questions: .........................................................................................................37

6. ENGINEERING & ADMINISTRATIVE CONTROLS ................................................................................ 38
ENGINEERING & ENGINEERING CONTROLS Questions: ..................................................................................39

7. PERSONAL PROTECTIVE EQUIPMENT (PPE) ..................................................................................... 40
LAB CLOTHING ..............................................................................................................................................40 LAB GLOVES ..................................................................................................................................................40 Safety eyewear .............................................................................................................................................41 PERSONAL PROTECTIVE EQUIPMENT (PPE) Questions: ..................................................................................44

8. WASTE MANAGEMENT ................................................................................................................... 45
WASTE MANAGEMENT Questions: ................................................................................................................46

9. SPILL RESPONSE ............................................................................................................................... 47
SPILL RESPONSE Questions: ...........................................................................................................................48

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STUDY GUIDE & PRACTICE QUESTIONS

INTRODUCTION
ALL students in introductory UCSD chemistry lab classes (CHEM 7L, 100A, 143A and 143AH) are required to demonstrate an understanding of general laboratory safety and familiarity with the UCSD Chemistry Teaching Lab Rules. A LAB SAFETY EXAM is given during the first week of class; a passing score on the exam fulfills this safety requirement. Students who FAIL to demonstrate an understanding of general laboratory safety and familiarity with the UCSD Chemistry Teaching Lab Rules may be dropped from the course with a grade of "W." This Study Guide is presented, in addition to Workshops, in response to the request of students. It must be considered a work in progress; we expect errors and omissions will be found and we plan to update it as needed. Please send us your corrections and suggestions, either for additional topics or for study questions. This Guide can only be an introduction to the study of laboratory safety, emergency response and hazardous materials management. We hope our student readers will develop a culture of safety that allows them to connect their chemical studies and their lab exercises with an assessment of chemical & physical hazards. We hope this text clarifies the logic of recommended safety protocols. As safety professionals, our goal is that our students will carry that culture of safety with them – to work and to home – and apply it to the management and storage of hazardous materials in a variety of settings. Study questions are provided after each section. It is hoped that these will provoke thoughtful responses, rather than rote memorization. If careful reading of the text doesn't supply a basis for a reasonable answer, please contact us for assistance (and to help us improve the Guide). Note that not all Safety Exam questions are included, although we've attempted to cover all pertinent topics. Group study is strongly recommended. Students who give each topic critical thought and suggest a reasonable answer to each question should be well prepared for the Safety Exam. Versions. The original Study Guide was a pilot project for Summer 2003. The Study Guide is updated periodically, as needed. Please send suggestions and corrections to the Teaching Labs Safety Coordinator for inclusion in future versions. Winter 2012 Sheila Kennedy, CHO Safety Coordinator, Teaching Labs Department of Chemistry & Biochemistry 1110 Natural Sciences Bldg., MC 0303 (858) 534–0221 John Palmer, PhD Safety Director Department of Chemistry & Biochemistry 2254 Urey Hall, Mail Code 0332 (858) 534-5906

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work and lives. the careful worker will ask "What would a prudent person do?" (i. non-absorbent shoes that cover the whole foot to protect feet from chemicals and broken glassware. drinks. pen and a water-proof marker for marking glassware. what would be the careful and sensible action?). Wear them only for lab work and remove them before leaving the lab area. Note particularly the rules concerning preparation and appropriate behavior. Review these guidelines frequently until these practices become habitual. Choose lab clothing carefully. Arrive prepared. On DAY ONE and every day. long-sleeve.e. hazards and the appropriate protection in your lab notebook as part of your pre-lab planning. Some have implications for both safety & scientific accuracy.. DRINKING. but check the local rules wherever you work. BASIC LAB PRACTICES EATING. 5 . AND SMOKING ARE FORBIDDEN in lab (to avoid chemical ingestion. and smoking materials (including chewing tobacco) should be left outside the lab or stored in securely closed containers away from lab work areas. To prepare for working in the Labs. Such “prudent practice” decisions save time. knee-length lab coat. Know the hazard(s) of each substance in your work plan – list materials. pack in a plastic bag to protect books & other items. lab notebook.STUDY GUIDE & PRACTICE QUESTIONS 1. In a situation not covered by these specific guidelines or the LAB RULES. When carrying a coat away from the lab. GUM CHEWING. Cover skin to protect from chemical spills and broken glass. LAB RULES & BASIC LAB PRACTICES LAB RULES for the UCSD Chemistry & Biochemistry Teaching Laboratories (the CHEM Teaching Labs) are posted in your lab (& included here). Understand the materials and equipment you will use in lab – contact your Instructor or TA for further information if the materials provided are not clear. excessive inhalation of harmful vapors and ignition sources). study the BASIC SKILLS and RULES outlined here. Many of the practices recommended have been developed for safety – to protect lab workers from accidents and contamination. Prudent practice. bring to class:     chemical splash goggles. Wear long trousers (or equivalent) and a long-sleeve lab coat. Students who arrive unprepared or inappropriately dressed will be dismissed. Food. Choose solid. Arriving prepared allows each student a full benefit of the lab experience. Remember that each organization or institution has its own set of rules – many will be the same or similar to ours. Others affect the precision and accuracy of the results obtained in lab exercises. Lab coats protect clothing from fine aerosols as well as larger spills. many of our guidelines reflect practices common throughout labs in industry & the research community. Appropriate clothing (see PERSONAL PROTECTIVE EQUIPMENT) protects the body & feet from accidental contact with hazardous materials.

CaCl2. Housekeeping. get a drink or have a snack.plan to take a break during the work period to remove goggles.STUDY GUIDE & PRACTICE QUESTIONS Avoid loose or synthetic clothing for lab work. Use paper toweling for absorbing hazardous materials. A graduated cylinder can be marked across the horizontal foot. clearly note the chemical you intend to put into a container. spills must be THOROUGHLY CLEANED (see SPILL RESPONSE for specific instructions). Your TA may instruct you in these techniques as well:  Spills on inert surfaces (for spills on people. In addition to causing disposal problems. 6 . see FIRST AID). etc. Use cotton towels to dry wet hands & clean surfaces. smoking and anything else that might increase the chance of ingesting lab chemicals. Clean your work area every day:     Clean hood areas and benches at the end of each session. Handle hazardous materials with correct techniques. store extra glassware and materials as soon as you finish with them. Left in the air. applying makeup. Dispose of dirty waste paper (towels. rowdy or boisterous play or pranks of any kind will be deemed cause for expulsion from lab. drinking. You know yourself best .  Label all containers before filling. and chemical contamination. Check to be sure all reagents and waste containers are securely closed. if needed.  Never remove chemicals from the laboratories. taking samples from lab creates the potential for an accidental exposure. The list of things not permitted in chemistry labs is long – begin with eating. Careful workers do not touch hands to their faces while working in lab. Regardless of how small. equipment. keeping only essential materials on the workbench. Keep work areas clear. MgSO4 or NaSO4) may absorb water from the air and MUST be kept tightly closed between uses. secure hair and clothing away from flames. cooking. NaOH or KOH pellets will absorb moisture and produce a puddle of concentrated corrosive liquid on the work bench – a serious skin exposure hazard. Do not attach samples to lab reports or notebook pages. Store backpacks and other extra materials away from work areas and off floors to protect them and to keep walkways clear. Professional and serious behavior is expected at all times. Clean lab surfaces with sponges. A laboratory is a workplace. Kimwipes.  Broken glass contaminated with hazardous or smelly materials can be rinsed with appropriate solvent before placing shards in the broken glass container. not in the general trash. Paper with absorbed hazardous chemicals should be placed in solid hazardous waste containers.) in trash receptacles. use tools such as tongs and scoops.  Never touch hazardous chemicals with bare hands.  Anhydrous materials (such as NaOH. remove loose jewelry. Using a waterproof marker or tape.

Hold the cap while pouring – ask your TA to demonstrate this technique.  Use a funnel when transferring liquids. stoppers and caps.  If no dedicated pipette is supplied. o Cap the bottle tightly & hold it over a beaker. place the cap on a watch glass or weighing paper. Don’t put any other pipette into a liquid reagent bottle. TECHNIQUE: Add a concentrated acid or base slowly to water with stirring. making sure to use the correct cap.  If a reagent spills down the outside of a bottle. After removing material from a container. TECHNIQUE: Avoid contamination of reagents: Dispense chemical only from bottles – never return them to bottles.  A liquid may have a dedicated dispensing pipette or syringe. then use a spatula to transfer what you need to your receiving container. Keep supply bottles and waste containers tightly capped at all times between uses. rinse the bottle with water. Build this habit from the beginning: in a research lab. this procedure avoids local heating and splattering of the corrosive material. o For inorganics. rinse the bottle with acetone. For a solid sample. Do not leave the excess material unattended and do not return it to the supply bottle. rinse the bottle.  The excess material left from using these techniques can be given to another worker. Transferring liquids. pour the approximate amount needed into a graduated cylinder or test tube. Bottle caps and stoppers can contaminate a workbench or hood surface.  Never return unused material to a reagent bottle. If the bottle is awkward or too large. replace the cap immediately. return your container to the balance. collect the rinse solvent and transfer solvent immediately to an appropriate waste bottle. 7 . Take care to keep the pipette with its bottle. the dated and signed laboratory notebook may become evidence of priority in a discovery. o For organics.  Never pipette by mouth. Record all data immediately in the lab notebook.  pour an approximate amount into a small beaker or watch glass. then pipette the sample into your flask or beaker. dispose of any remaining excess in the excess material container provided. Any copying from temporary notes risks introducing errors in recorded data.STUDY GUIDE & PRACTICE QUESTIONS  Bottles.

Use the brush to clear the balance area. Note the maximum fill line for each container. NEVER FILL THE BOTTLE ABOVE 90% of the container volume. Close a full bottle & leave it in its tray. stopcock grease or glycerin and protect both hands with paper or cloth toweling.” assume all experimental wastes are hazardous and look for appropriate waste containers (see WASTE MANAGEMENT). Work in a hood when handling volatile materials that are toxic. Request a replacement waste bottle from the Stockroom (YORK 3150 or NSB1104).  Remember to calibrate and tare the balance – ask the TA for instruction. On each hazardous waste container is a clear description of the material(s) it should contain.  Never leave any solid or liquid in or around the balance area. When inserting a thermometer or glass tube into a rubber stopper. Remove the container from the balance to add loose material. corrosive. Thermometers and glass tubing. 8 .STUDY GUIDE & PRACTICE QUESTIONS Balances. The vented laboratory hoods provided in the Teaching Labs are an integral part of the air handling system (see ENGINEERING CONTROLS). Select smooth paper or a small beaker. lubricate the glass with water. Laboratory hoods.  Clean the balance area after each use. according to the size of the sample needed. return it to the balance and record the mass in your notebook. Hoods provide a safe work area for volatile hazardous materials. Air flows into the hood through and around the face frame and carries vapors and fumes out of the laboratory. flammable or odorous. Unless you have specific instructions to “dispose to drains. Each lab exercise in the Teaching Labs has specific instructions about proper waste handling and disposal. Waste disposal. Grasp the thermometer or tubing near the stopper and push gently with a twisting motion. Put waste materials in the PROPER container. TECHNIQUE: Do not place loose powders directly on balance pans.

. All students. and ignition sources). Contact lenses: Worn with safety eyewear (required for everyone). Avoid loose or synthetic clothing. GUM CHEWING. Always be sure to use proper eye protection with ultraviolet (UV) lamps and lasers. DRINKING. faculty. Clean your own work area and any common areas assigned to you. individual Instructors may use a more restrictive rule (e. drinks. long sleeve lab coat closed to protect skin & clothing. KNOW THE HAZARDS OF MATERIALS before beginning any procedure. AND SMOKING ARE FORBIDDEN in lab (to avoid chemical ingestion. staff. Wear a knee-length. and smoking materials (including chewing tobacco) should be left outside the lab or stored in securely closed containers away from lab work areas. SAFETY EYE PROTECTION: [Note: this is the Department rule. Check the appropriate Material Safety Data Sheet (MSDS). Students who arrive unprepared or inappropriately dressed may be dismissed until ready to work. and chemical contamination.g. contact lens wear is acceptable.] Safety Eye Protection must be worn by everyone when anyone in the lab works with glassware or chemicals.STUDY GUIDE & PRACTICE QUESTIONS LABORATORY REGULATIONS UCSD Teaching Laboratories Prepare carefully. long pants. check with Safety Coordinator or Instructor. Approved goggles and glasses. Coat sleeves must cover arms & shirts. additional information is available on bottle 9 . secure hair and clothing away from flames. Long Pants and Closed Shoes are required. Prepare & Protect Yourself The minimum level of safety protection needed in the Teaching Labs is safety eyewear. and long lab coat. The Teaching Labs Stockrooms are not equipped to lend or sell goggles. EATING. GLOVES are provided in the labs and should be worn when working with hazardous chemicals. Safety glasses are designed for use in normal laboratory operations but offer only minimal splash protection. Remove gloves and wash hands before leaving the lab and entering public areas.. excessive inhalation of harmful vapors. remove loose jewelry. equipment. in addition to any prescription glasses. Additional eye and face protection (e. For goggles from other labs/schools/activities. Wear long pants (or equivalent) to protect from spills & splash. Chemical splash goggles are required whenever anyone is transferring more than a small amount (~25 mL) of a hazardous material or when performing any operation involving a splash hazard. choose safety glasses designed to fit over glasses or choose goggles. The current understanding is that using contact lenses in lab creates no additional hazard. and visitors are required to wear approved splash goggles or safety glasses. Food. Ask the lab staff if you do not find suitable gloves stocked in your classroom. APPROPRIATE CLOTHING: Lab Coats. Attentive and considerate behavior is expected at all times. Maintain clean laboratory benches and common areas. Choose sturdy shoes that cover the whole foot and protect from spills and broken glass. goggles only). Chemical splash goggles (close fitting & indirectly vented) are required for some classes – check the syllabus. With prescription glasses. full-face shields) are available and used as directed by the experimental procedure or the lab supervisor.g. closed shoes.

in the Science & Engineering Library and in the Chemistry Teaching Lab Stockrooms (YORK 3150 and NSB 1104). 10 . Learn how to summon assistance from the Stockroom. location of first aid station(s). in the laboratories. EMERGENCY RESPONSE INFORMATION forms allow students & staff to communicate medical information to emergency responders. the Instructor. blank forms are available in the Teaching Labs Stockrooms. telephones.. consult with the lab staff. whether a telephone is installed. learn the exits & evacuation routes. Campus Police. Date containers when first opened. KNOW YOUR SAFETY EQUIPMENT: In each lab. To obtain a refill from the Stockroom. Never pipet or start a siphon by mouth. do not dispose of any waste to the drains. campus EH&S. The Environment. HAZARDOUS WASTE MANAGEMENT. Work stations for physically impaired or temporarily disabled students are available. or member of the lab staff. If you carry medication that might be needed on an emergency basis (e. shower/eyewash stations. request a refill and carry the filled container back to lab in the secondary container. if you need these facilities. HAZARDOUS MATERIALS HANDLING: Label all containers with contents (material & concentration) and chemical hazards. Prevent Accidents and Spills Attentive and considerate behavior is expected at all times. Unless explicitly instructed. or the lab staff person for your course. spill control materials & fire extinguishers. Students are to be in the Teaching Labs only when attended by an Instructor. in your laboratory manuals & textbooks. Horseplay and pranks are especially dangerous in a laboratory setting and are forbidden at all times. Special secondary containers are provided for carrying hazardous materials outside the lab or between labs. and your health care provider. Hazardous waste containers are provided. inform your lab supervisor or a responsible coworker. ask your Instructor. Never work alone in the laboratory and never perform unauthorized experiments. Store hazardous materials below eye level and return materials to their proper storage locations. for diabetes or asthma). as appropriate (see below). TA. headphones and ear buds) are forbidden. Distractions (such as music. this has been a source of serious laboratory mishaps. Return the filled bottle & the carrier to their storage locations. KNOW YOUR OWN LIMITS: If you have limited mobility or any condition that may limit your ability to work safely. choose the correct container for chemical hazardous waste and for all broken glass (& other sharps). Keep all lab materials and lab gloves away from the face & mouth.g.STUDY GUIDE & PRACTICE QUESTIONS labels. Store hazardous materials in secondary containers (trays or tubs) and segregate materials according to hazard classes. At the Stockroom. choose the appropriate secondary container to carry the empty bottle. Health and Safety Specialist at the Teaching Labs (see below) or the lab safety staff at UCSD EH&S (x 43660) can also help you find information. Read labels and ask the TA. Maintain clean laboratory benches and common areas. as they may distract the user from the task at hand or prevent the user from hearing instructions or warnings. or EH&S.

Call the Teaching Lab Stockroom or send an uninjured person with a message. fire extinguishers. shut down gas lines & heat sources. Pull the fire alarm as you exit. Exit the building quickly (see BUILDING EVACUATION). remove contaminated clothing. assist the exposed person to the shower/eyewash and rinse the areas of contact with copious amounts of water for 15 minutes or until assistance arrives. Call 858-534-4357 or send an uninjured person to notify the lab staff to ensure injuries receive proper treatment. Emergency contact information is posted near each telephone (where available). coat. or Sudden Illness **** TA must not leave students unattended in the classroom. Provide assistance to injured persons. near misses. Choose a sheltered position to wait (under a table. respond immediately: douse with shower or smother with fire blanket. call 858-534-4357 and report the situation to the UCSD police. in the frame of a closed door. FIRE: For clothing fire. Note any injuries to yourself or others and any remaining dangers. or problem areas. For large or very hazardous spills. store in the Hazardous Waste Area of the lab. Notify the lab staff – disposal will be arranged. Never mix mercury with other waste. Go to the assigned location for your lab or building. SPILL CLEANUP: Do not attempt without proper protective equipment. corrosive. Do not attempt to fight equipment/property fires in the lab. If you suspect an ambulance is needed. When in doubt. At a safe location. as long as you do not place yourself in additional danger. fuming or noxious chemicals. evacuate the lab quickly (see BUILDING EVACUATION). These records are important in identifying recurring injuries. Lab supervisor will take attendance (to assure everyone is safe) and provide this information to responding emergency personnel. PERSONAL EXPOSURE: If clothing catches fire or if a hazardous chemical is spilled on skin or in eyes. BUILDING EVACUATION: Always assure the safety of people before considering any damage to property. Use stairs. leave the lab immediately. An ACCIDENT REPORT is necessary for any accident or chemical spill.STUDY GUIDE & PRACTICE QUESTIONS Use LABORATORY FUME HOODS for all work involving (or producing) flammable. no matter how minor the incident seems. EARTHQUAKE: Move away from overhead lights. When instructed. do not hesitate to call for assistance: 858-534-4357. Respond Appropriately to Accident. heavy unsecured objects. Once tremors stop. For a mercury (Hg) spill use only mercury collectors provided in spill kits. or towels. call for assistance. and hazardous materials. use the spill cleanup kits and PPE provided. call for assistance. never elevators (power may fail in an emergency). close doors and call for assistance. or against a bearing wall). Spill. consult your lab supervisor and Material Safety Data Sheets for cleanup precautions. **** SUMMON ASSISTANCE and – if you are trained – ADMINISTER FIRST AID. place the work in a hood and read MSDS. 11 . Any volatile toxic substance should be opened & used only in a laboratory hood. Do not leave the area or reenter buildings until instructed to do so. For small spills. Double bag and label contaminated materials.

and smoking materials in the labs?  What clothing is appropriate for CHEM lab workers?  Where should books be stored in lab?  What does “prudent practice” mean?  Proper lab techniques often have both scientific and safety reasons. Which of the following items has both significant scientific and safety implications?  Wearing closed shoes and safety eye protection  Opening chemical bottles carefully and transferring chemicals without spills  Noting numerical experimental results with appropriate significant figures  Carefully reading names and other label information on chemical reagents used in experimental protocols  Why is the practice of using a spatulas to scoop powder from a bottle discouraged? What’s a preferred technique?  What should you do with excess materials you won’t use?  What kind of work is best done only in a fume hood?  Check dictionary definitions: distinguish clearly between “volatile” and “hazardous.” Are all volatile materials hazardous?  Our LAB RULES are posted in the labs & available on our website.STUDY GUIDE & PRACTICE QUESTIONS LAB RULES & BASIC LAB PRACTICES Questions:  What is the reason for prohibiting ALL food. drink. How could you find the RULES for another lab you planned to visit? 12 .

or ether) away from the heat/ignition source (match. is a useful lab tool and has many applications. once heated by the flame. an ignition source and a reaction mechanism. for lab clothing. Choose flame retardant natural fibers. the flammable solvent should not have been used in the same area as a hot plate. wool and linen. Manage long hair to prevent loss or contamination. Synthetics fibers tend to burn easily or melt onto skin and are particularly inappropriate for lab wear.STUDY GUIDE & PRACTICE QUESTIONS 2. remove loose jewelry and restrain loose clothing. the fire can be stopped (or prevented) by keeping the fuel (wood. very difficult to extinguish. In any of these situations. the fire might consist of natural gas. gas. (In the last case. under careful control. In an "ordinary" fire. Keep containers of flammable materials closed. Synthetic (acrylic) fingernails have been tested for flammability and are surprisingly flammable and. spark. If any one of these is missing. a fire will die or fail to ignite. such as cotton. 13 . An open flame. an oxidant. once ignited. manage gas hoses and store books away from work areas. If many flammable materials are present. and the ignition source could be a match. the fuel could be wood or paper. Anticipate working with open heat sources when preparing for lab. the oxidant could be oxygen from the surrounding air. ambient oxygen and a spark from a flint lighter. FIRE SAFETY FIRE PREVENTION Fire Tetrahedron: Four elements are necessary for a fire:     a fuel. The four sides of the solid tetrahedron represent the four necessary elements of a fire. hot plate). or the vapor from an open beaker of ether plus ambient air and the heat from a hot plate. set aside a limited area of the lab for open flames and keep all flammable materials away from the flame area. Remember that equipment such as tripods and ring stands. Organize work to leave substantial space between the burner flame any materials that are not intended to be part of a reaction: restrain sleeves & hair.) FIRE TETRAHEDRON. remain hot for some time. Removing any one side destroys the solid shape. In the lab. In lab. These items can catch fire or contact hazardous chemicals. Understanding this gives you a way to think about how a fire may progress.

gas.. paint) energized electrical equipment Other classes exist. Fire extinguishers are placed in the labs to be used by trained personnel – always working in pairs. Smother the fire with any item available (coats.) according to the type of fire it is designed to extinguish. assisting others where possible.).STUDY GUIDE & PRACTICE QUESTIONS FIRE RESPONSE Always consider the safety of people as your first priority in any emergency response. but these are the ones of concern in our introductory courses. Once students are safe. as are some of the lab assistants. if you can do so safely and without delay (see BUILDING EVACUATION). Clothing or hair on fire is a serious emergency that requires immediate response from the nearest unaffected person. jackets.g. Shut down any open flames or electrical equipment in your area. It is not expected that students have been trained & they are not expected or encouraged to use fire extinguishers. etc. An ABC combination extinguisher can be used on any or all of these fires. Students should learn about extinguishers as part of basic safety training and take advantage of any opportunity to be trained in their use. B. blankets. Use any means at hand to prevent the victim from running. sweatshirts. as the victim will be unlikely to remember previous training. but the nearest available item should be used to respond without delay. 14 . Lab TAs are trained in the use of fire extinguishers. C. The Class B extinguishers provided in the organic chemistry labs contain carbon dioxide (CO2) under pressure. never alone.. Learn the mnemonic for each to help you remember the types: EXTINGUISHER CLASS: Class A (Ash) Class B (Burning liquids) Class C (Current) for fires involving: ordinary combustible materials (e. Each fire extinguishers is designated (A. etc. Fire blankets are provided in the labs for this purpose. organic solvents.g. persons trained in the use of fire extinguishers can consider using fire extinguishers. trash. as running feeds the flames and makes the fire worse. wood or paper) burning liquids (e. A student's normal response will be evacuation of the area. It contains a dry chemical powder.

containing all the lab notes for a long-term research project)?  What kind of fire extinguisher would be chosen to extinguish a fire involving an organic solvent (e.STUDY GUIDE & PRACTICE QUESTIONS FIRE SAFETY Questions:  What should you do if you observe a fire in an unattended hood in the lab?  What would be the effect of covering a beaker of burning acetone with a large watch glass?  What kind(s) of fire extinguishers are supplied in your lab?  How many of each kind?  For what kind of fire is each intended?  Which of those fire extinguishers would a trained worker choose to extinguish a paper fire (for example.. a research notebook.g. ethanol or acetone)? 15 .

STUDY GUIDE & PRACTICE QUESTIONS 3. It is the responsibility of each lab worker (student. plumbing or electrical problems. staff. the information will be passed only to Emergency Responders. if needed. This information (allergies. You may have information that should be given to an Emergency Responder (paramedics or emergency room staff) if you are injured or involved in an accident and not able to respond.  fire extinguishers &  shower/eyewash. etc. always safeguarding lives and the safety of people first. Careful storage protects workers from tripping and protects books and other materials from contamination. such as corrosive liquids or flammable products (see WASTE MANAGEMENT) so these items do not cause injury. Carry a copy in your wallet or backpack. TECHNIQUE: Call for emergency assistance 858-534-4357 Campus emergency operator will send help for medical emergencies. 16 . If needed. Each lab is provided with a storage area for backpacks & other personal items away from work areas and off lab floors.  telephones (if any). fires. TA or Instructor) to know his/her role in an emergency and respond appropriately. Begin by studying the equipment provided in the Teaching Lab (and in each new lab you enter). PREPARE TO RESPOND Learn emergency equipment. Anyone carrying emergency medication should make the lab supervisor or another student aware of its location so they can assist. The Lab Staff strongly encourages everyone to complete this form and make it available by securing it inside the back cover of the student lab notebook. Learn how to call for help. Prevent Injuries and Spills Dispose of broken glass and other hazardous items. EMERGENCY & DISASTER RESPONSE An appropriate response to any emergency always considers people before property. when needed.) can be recorded on an EMERGENCY RESPONSE INFORMATION form. medications.  first aid & spill kits. Dispose of all nonhazardous rubbish in the trash cans and keep floors clear & dry at all times.  fire blanket Learn to use these item: listen to your TA’s introduction and read the sections on FIRST AID and FIRE SAFETY. Learn the locations of the:  exit doors.

if available) to Emergency Responders. TA and involved persons will complete an Accident Report. If your assistance isn’t needed at the moment.  DROP: Dropping to the floor allows the next step.STUDY GUIDE & PRACTICE QUESTIONS CLOTHING FIRE: STOP! – DROP! – ROLL! Fire on Clothing or Hair is a serious emergency that requires immediate response from the nearest unaffected person. remain available to give assistance if needed. as the victim will be unlikely to remember previous training. An injured person may not remember training or may be unable to listen to instructions. ask in the Stockroom. if it is nearby. those nearby must respond and give what aid they can whenever doing so does not increase danger to others. Waiting outside the lab provides privacy.  Emergency shower/eyewash is a good response to a fire. sweatshirts. Do not travel more than a step or two to reach it . Use whatever is at hand to help. If emergency shower is in use. etc.  ROLL: Roll the affected person to smother flames. blankets. floors will be slippery. Do not wrap a person who is on fire and still standing – forming a chimney will promote the fire. through the University. jackets. 17 . Everyone should take an opportunity to be trained in at least Basic First Aid. Don’t do anything that will increase the number of injuries.) can be used to smother a fire once a person has dropped to the floor. and by the American Red Cross. if extra dry clothing is needed. Classes are offered through various community organizations. Seek medical assistance and give all information (including the nature and concentration of the spilled material plus the injured person’s EMERGENCY RESPONSE INFORMATION form.  STOP: Prevent the affected person from running. use any means available to stop him/her. Those not directly involved in the response should keep the area clear for responders to work.STOP! – DROP! – ROLL!  Fire blankets – or any other available item (coats. Call for assistance (858-534-4357) & notify Lab Staff. FIRST AID A lab injury or a sudden illness requires an alert and immediate response from nearest uninjured persons on the scene. as running feeds the flames and makes the fire worse. Since minimizing time before aid is critical in minimizing injuries from burns and chemical spills.

Once the contaminated clothing is away from skin surfaces. begin CPR where needed  prevent further injury/contamination. Identify possible chemical contamination or exposure and give information (including MSDS and EMERGENCY RESPONSE INFORMATION form) to Emergency Responders. First responder is the nearest person on the scene (student. Request replacement first aid items to restock the First Aid kit & leave it ready for another class. apply pressure to stop bleeding. TA. Instructor or staff):  call for assistance.  loss of consciousness. Wash cuts gently.  notify Lab Staff  apply pressure to stop bleeding and. SUDDEN MAJOR ILLNESS Major illness may include  serious cuts or burns. If serious injury is suspected. even for the most minor injuries.  seizure. Don’t wait to open buttons. An uninjured person must keep time. HAZARDOUS CHEMICAL SPILL ON SKIN OR IN EYE In the best spill situation. WATCH THE CLOCK. RINSE with water only – no soap or scrubbing. Use the blanket to cover a person who is ill or injured to keep them warm & prevent shock. often before liquids penetrate.  Gently hold eyes open with fingers. TA/Instructor will complete an Accident Report. evaluate whether there is a skin exposure (or just a laundry/disposal problem). Fire blankets are provided in the labs. TA and injured student work together to complete an Accident Report (available in the lab) and return the report to the Stockroom.STUDY GUIDE & PRACTICE QUESTIONS MINOR INJURIES: BURNS & CUTS Notify TA without delay.  if trained. continue rinsing until paramedics arrive on the scene and begin medical evaluation.  difficulty breathing. 18 . a chemical lands on clothing that can be quickly removed – lab coats are especially easy to peel away. Apply bandages in First Aid kits. Continue rinsing for 15 minutes – no soap or scrubbing.  any head wound. Begin immediately and continue rinsing for 15 minutes. EYEWASH provides direct rinsing for face & eyes:  Begin rinsing with goggles on  Remove goggles and continue rinsing. Identify possible chemical contamination or exposure and seek First Aid:     Cool burns and dilute/remove contaminates with cool running water.

The CHEM Teaching Labs do not conduct fire drills. even in dangerous buildings.  Remove contaminated shoes and clothing while rinsing to separate contamination from skin surfaces. especially with stairs. keep your body low and protect your face and neck.).STUDY GUIDE & PRACTICE QUESTIONS EMERGENCY SHOWERS rinse the whole body. A complete accounting is essential. Lab Staff will call Facilities Management to deal with the water. TA or Instructor will take roll to assure that everyone is safely evacuated – be sure you are counted. Be aware that light fixtures may swing loose and watch for unsecured equipment that may be shaken off a table or bench. Report any information you have about damage to the building or about the situation in the building (location of the fire. may require BUILDING EVACUATION. don’t try to get up.  Exit the building quickly. soaking anything on the floor. use it to rinse off contamination on skin or extinguish fires on clothing. If the first jolt knocks you down. When the first shaking stops. evacuate as for a fire. you can take information to Emergency Responders. 19 . turn off gas flames and electric heaters and gather your personal belongings (you may not be able to re-enter the building).  Notify a Emergency Responder if anyone is missing. such as a major chemical spill.  If you can do so without injuring yourself. a fire or an earthquake. When you hear an alarm. Remember that your first priority is always the safety of people. if both are needed. Do not leave the area or re-enter the building without explicit instructions. always assume it is real. and away from overhanging power lines and tree branches. BUILDING EVACUATION A major event. An earthquake evacuation is a special case: wait until the shaking stops.  If you can’t help. etc. expect the water to pool in the lab. as Emergency Responders will search for missing persons.  Gather your group at your assembly location. assist others.  Use only the stairs – remember that power(& elevators may fail. Paramedics) to work. clear of Emergency Responders’ operations. chosen to allow groups to gather away from buildings. If you can do so safely and without delay. Walkways will be slippery. report o what help is needed o where the person is located. shut down open flames or electrical equipment in your area. paying special attention to reporting damage and unsafe conditions. cool burns and dilute/remove contaminates. It provides unheated domestic (drinking) water in massive quantities. but shelter against a wall and away from windows or other glass that may shatter. Fire Fighters.  If you can do so without delay. Follow all instructions of Emergency Responders.  Use with eyewash. Your assembly location may be moved to allow Emergency Responders (Police.  No drains are provided. Each University building has an assigned assembly location.

ongoing medical concerns. medication. revised/replaced as needed. bites. etc.. and history of serious illness/seizures/fainting. Students: Tape this form INSIDE THE BACK COVER OF THE LAB NOTEBOOK. plants.).STUDY GUIDE & PRACTICE QUESTIONS EMERGENCY RESPONSE INFORMATION Students & staff may. a responsible person who should be notified if you are ill/injured) Name ____________________________________________ Address __________________________________________ __________________________________________________ Relationship ______________________________________ Phones ____________________________________________ ADDRESS __________________________________ ___________________________________________ PHONE ____________________________________ DATE OF BIRTH _____________________________ UCSD ID # _________________________________ MEDICAL INFORMATION (List ALL allergies (food.) DO YOU WEAR CONTACT LENSES? (important in case of eye injury) Yes __ No __ CURRENT MEDICATIONS (Copy information from medication labels for all medicines – prescription & over-the-counter. If you are ill/injured and cannot respond. 20 . provide information to assist responders in case of emergencies. at their own discretion. NAME _____________________________________ EMERGENCY CONTACT (i.e.) Name of medication Dose Frequency example: Tetracycline Sudafed 250 mg 200-400 mg twice/day as needed for sinusitis headache _______________________________________________ _______________________________________________ _______________________________________________ _______________ _______________ _______________ ____________________________________________ ____________________________________________ ____________________________________________ EMERGENCY RESPONSE INFORMATION forms are available in Teaching Labs and in the Labs Stockrooms. Staff: carry in wallet/backpack. this information will be provided to emergency responders (paramedics/hospital personnel).

21 . Follow instructions of Emergency Responders. Return to building only on instructions of Emergency Responders EH&S Staff Lab Staff. Assist others where possible – report injuries to Emergency Responders. Assemble your group between Pacific Hall.STUDY GUIDE & PRACTICE QUESTIONS BUILDING EVACUATION Natural Science Building Pacific Hall Urey Hall Natural Sciences (NSB) ASSEMBLE HERE N Shut down open flames & electrical equipment in your work area. Close doors as you leave. NSB and Urey Hall – keep all driveways clear. Take roll & report missing persons to Emergency Responders. Use stairs only – elevators may fail.

Use stairs only – elevators may fail. YORK HALL Shut down open flames & electrical equipment in your work area. Follow instructions of Emergency responders. Gather your group in Revelle Plaza – take roll & report missing persons to Emergency Responders.STUDY GUIDE & PRACTICE QUESTIONS BUILDING EVACUATION York Hall REVELLE PLAZA ASSEMBLE HERE GALBRAITH HALL N Close doors as you leave. Assist others where possible – report injuries to Emergency Responders. 22 . Return to building only on instructions of Emergency Responders EH&S staff Lab Staff.

23 . lab partner or lab supervisor) of its use & location?  How does building evacuation for an earthquake differ from evacuation for a building fire?  How often does the Chemistry Department run fire drills in the Teaching Labs?  Where does your class assemble in an evacuation? Why?  If you get a chemical splashed in your eye(s).STUDY GUIDE & PRACTICE QUESTIONS EMERGENCY AND DISASTER RESPONSE Questions:  What is an EMERGENCY RESPONSE INFORMATION form and who should use one? Where can you get a replacement if yours is lost or needs revision?  Suppose you carry emergency medication that may be needed on short notice. What are the pros and cons of informing others (roommates. you should flush your eye(s) with running water for at least how many minutes?  Where is the First Aid kit in your lab stored?  Where is the fire blanket? Why was that location chosen?  What might be your role in an emergency response if you were the nearest uninjured person? If you are at the other end of the room?  Who should be notified in case of a sudden illness or injury in the lab?  Suggest appropriate situations in which the emergency shower/eyewash would be helpful.

The associated risk of being injured by a hazardous material is a combination of the inherent hazard and the degree to which a worker is exposed. corrosivity. there is no risk of injury. such as sharp edges. each of our students becomes a resources in the community. especially small children. The hazards inherent in the materials do not change with location or intended use. Check your garage and cleaning supplies for labels that warn (often in very small lettering):  "wear gloves" or  "use only with adequate ventilation. Once trained.STUDY GUIDE & PRACTICE QUESTIONS 4. 24 . a manufacturing shop. When there is no exposure. Understand the hazard – then minimize the exposure to minimize the risk. it may be a hazardous material if it presents physical or electrical hazards. reactivity (including radioactivity). extreme temperature. Hazardous materials found in homes present a special hazard to untrained family members. In addition. whether found in a chemistry lab. UNDERSTAND THE HAZARD – THEN MINIMIZE THE RISK A hazard is an inherent property of the material and not generally something that can be changed. HAZARDOUS MATERIALS A substance is called a hazardous chemical if it has one or more of the following characteristics: toxicity. able to educate friends and family member about hazardous materials and how they can protect themselves." These materials (from razor blades to concentrated acids) should always be handled with care and disposed of properly. or a home cleaning or hobby cupboard. or high voltage. flammability. Hazardous materials are found in nearly all workplaces and in most homes & hobby shops.

o a simple asphyxiant (such as CO2) displaces oxygen in the air. NH2NH2). Many common solvents are highly flammable and require special care. -NO3) or strong reducers (such as hydrazine. Flammable (ignitable) materials are those that readily ignite & burn in air.STUDY GUIDE & PRACTICE QUESTIONS HAZARD IDENTIFICATION Hazardous Chemicals are TRICKY Materials. Uncontrolled reactions may cause explosions or start fires. Other materials are stable in themselves. Reactive materials are those likely to react spontaneously under normal (or relatively mild) conditions. Hazardous chemicals fall into one or more of the following groups:     Toxic Reactive (including radioactive) Ignitable (flammable) Corrosive The mnemonic TRICky (from the initial letters) is a reminder that these materials may have some surprising properties. a useful oxidation reaction may proceed slowly under controlled conditions or explosively when uncontrolled. as well as those that react spontaneously with water or air. Reactives may include oxidizers (such as nitrates.  Allergens may cause reactions in people who have become sensitized through previous exposure. any workplace in which you will use such materials will be required to provide training in the appropriate use & precautions. Toxic materials cause poisoning or illness through a wide variety of routes. as are mutagens and teratogens. Characteristics which are useful in controlled chemical reactions may become injurious if allowed to proceed in contact with living tissues or if the reaction proceeds more quickly than desired. Radioactive materials are a special case of reactive materials. careful storage of hazardous materials keeps incompatible materials separated. but must be kept apart from materials with which they would react strongly (incompatible materials). Thus. One material may fall into any combination of these groups. even if the bottles were broken. Radioactive materials emit ionizing radiation as unstable atomic nuclei decay to form more stable nuclei.  Asphyxiants interfere with the supply of oxygen to vital organs: o a chemical asphyxiant (such as CO) deprives the body of oxygen. 25 . The Teaching Labs do not currently use any radioactive materials. Ionizing radiation may also be emitted by machines such as X-ray machines. acids and bases are stored in such a way that they would not mix. the emitted radiation (which may occur in several forms) may damage living tissues or effect changes in nearby materials. a reaction of a base with a fat – used commercially to form soap – will also cause a deep penetrating chemical burn if the fat involved is a part of your skin or eye.  Irritants cause transient effects which may be mild or serious. Some special cases include:  Carcinogens (materials that cause cancer) are a special group of toxic substances. For example. In another case. The materials and the hazards don’t changed – just the exposure. Toxicity is a difficult hazard to quantify and predict.

These include razor blades. falling. Thermal hazards arise when materials are substantially hotter or colder than ambient (surrounding) temperatures.  Keep walkways clear and keep floors dry. TECHNIQUE: Clean broken glass shards from floors/tables with a brush & dust pan. hold a hand several inches away and slowly move the hand closer until you can feel heat radiating from the surface. TECHNIQUE: To detect whether an item (such as a beaker) is hot. H2SO4) or bases (such as sodium hydroxide. Use tongs. as well). Set hot glass in a labeled area. check all electrical cords and replace worn ones immediately. damaged or improperly grounded instruments. NaOH.  Restrain hair and clothing. This practice protects lab workers as well as others who may enter the lab. Carefully dispose of sharps in the special containers provided in the labs – never to the ordinary trash. Strong bases are a particular eye hazard (see the reaction between fat & base. such a liquid nitrogen or dry ice (CO2(s)). on a mat.  Read and understand the instructions for any machine before using it. above). Risk of electrical shock arises when current runs in unplanned ways. Remember that hot glass looks just like cool glass. tripping. This may result from worn. Injuries from slipping. crushing and cutting are among the most common in American workplaces. Carry the sharps to the BROKEN GLASS BOX without touching the glass with hands. Use similar precautions to protect skin surfaces when working with very cold (cryogenic) materials.  Do not operate instruments on extension cords. or ammonium hydroxide.STUDY GUIDE & PRACTICE QUESTIONS Corrosives are materials (solids. These may be acids (such as hydrochloric acid.  Do not overload circuits. or sulfuric acid. needles. fine pipettes. Physical/mechanical hazards are common in labs and other workplaces. HCl. or in a second heat-proof container while it cools.  Manage cords to reduce mechanical wear and restrain excess lengths (preventing trip hazards.  Manage electric cords to keep them clear of walkways and work areas.  Read and understand the instructions for any machine before using it. liquids or gases) that cause corrosion on metals or 'eat away' living tissues. 26 . and any broken glass. Sharps are any items that could cut through a plastic trash bag. insulated gloves or other aids to guard hands and other skin surfaces from contact with extreme temperatures. These materials are very difficult to wash from eyes. NH4OH).

but may contribute to the formation of an ignitable mixture. carbon disulfide (CS2) has an ignition temperature of 95C and can be ignited by a glowing light bulb. it's difficult to quantify or predict toxicity. causing a fire which might spread to other materials. Toxic materials cause poisoning or illness. If a room-temperature spill of acetone produced a mixture of 10% acetone in air in the immediate area of the spill. in itself. an indicator of a high hazard. observing effects with increasing doses. The ignition temperature (or autoignition temperature) is the point at which the substance (liquid.6% and 12.8% in air. we can observe that one material is substantially more toxic than another (has a smaller LD50) in mice (and should therefore be handled appropriately by those caring for mice). As simple as that statement seems. From this kind of data. The dose at which half of a population of mice dies of an ingested toxin is shown as: LD50. treating it as though it were toxic to humans as well. each material has upper & lower flammable limits. In such situations. mice or rats. Similarly. No spark is necessary for ignition when a material reached its ignition temperature: diethyl ether (H5C2OC2H5) has an ignition temperature of 160C and can be ignited (liquid or vapor) on the surface of a laboratory hot plate. generally with large populations. Flammable materials The flash point of a material is the temperature at which the liquid has sufficient vapor pressure to form an ignitable mixture in air near the liquid surface. Other data can be gathered by testing materials on such species as fish. however. mouse. mice and rats. as the vapor mixes with surrounding air. any spark or other ignition source might ignite the flammable mixture (acetone and air). a toxic dose (or concentration) for 50% of a population is expressed as TD50 (TC50). an extreme fire hazard would exist until the acetone was cleaned to less than 2. acetone (a common and useful laboratory solvent) has a flash point of -18C and lower & upper flammability limits of 2. or gas) has enough energy to initiate self-sustained combustion. but we can't tell anything directly about the toxicity of either material in humans. An LD50 will be expressed in units of mass of toxin (mg or μg) per unit of body mass (g or kg). 27 . solid. called Minimata disease) or industrial exposures (such as asbestosis in asbestos miners or black lung disease in coal miners). decide to be very careful with a material which is toxic in fish. Much of what we know about human toxicity has been learned from the investigation of environmental contamination (such as mercury poisoning. it's often difficult or impossible to determine the threshold limit (the concentration at which an effect is first observed). Until that time. In addition. ingestion. For example.STUDY GUIDE & PRACTICE QUESTIONS HAZARD ASSESSMENT Some indices help us to assess the level of hazard present in a particular material. many common materials have flash points below common room temperatures. We may.6% in air.’ Relatively high volatility is not. A material with a high vapor pressure is termed ‘volatile.

(CH3CH2)2NH. They are often the anions of weak acids Examples include:  ammonia. The more extreme the pH value. A strong acid or base solution can be either concentrated or dilute. Dip a clean glass rod into the solution in question. Hydrofluoric acid. but is hazardous because of its toxicity. the stronger the acid or base. 28 . HF. (from citrus fruits: 2-hydroxypropane-1.STUDY GUIDE & PRACTICE QUESTIONS Corrosivity is assessed by testing the pH of a solution (or a solution made from the dry material):  A neutral solution has a pH of 7.  A pH value < 7 indicates an acid solution. Touch the drop on the end of rod to the pH test strip and compare the resulting color of the strip to the guide on the package label. The common strong acids are completely dissociated (in 1M aqueous solution) and have very low pH (pH < ~3):       HCl – hydrochloric acid HNO3 – nitric acid H2SO4 – sulfuric acid HBr – hydrobromic acid HI – hydroiodic acid HClO4 – perchloric acid Weak acids give pH values between ~3 and 7. and  diethylamine. NH3. TECHNIQUE: To determine whether a liquid is corrosive. Strong bases are completely dissociated (in 1M aqueous solution) and have very high pH (pH > ~10):      NaOH – sodium hydroxide KOH – potassium hydroxide LiOH – lithium hydroxide RbOH – rubidium hydroxide CsOH – cesium hydroxide Weak bases give pH values between 7 and ~10. Note that the terms ‘strong’ and ‘weak’ do not refer to the concentration of a solution.3-tricarboxylic acid) and acetic acid (found in vinegars. set a pH test paper (available in labs) on a watch glass.2. Examples of weak acids include citric acid.  A pH value > 7 (up to 14) indicates a basic (alkaline) solution. is also classified as a weak acid. CH3COOH). Review a general chemistry text to be sure you understand these terms.

STUDY GUIDE & PRACTICE QUESTIONS HANDLING & STORAGE Hazardous materials storage plans reflect our understanding that various chemical classes will react with each other. these areas are either not earthquake safe or materials stored there might interfere with fire sprinklers or other essential systems. you may run out of a necessary reagent. Despite our best efforts to have the materials you need ready in the lab. Reduce the chance of unintended reactions by:      storing bottles in secondary containers (trays or tubs that will contain spills) store incompatible items (acids & bases or oxidizers & fuels) separately Earthquake strips on shelf edges keep things from falling during a tremor. Flammable materials. Bottle caps and cupboard doors should be closed whenever not in use. The secondary containers (red bottle jackets and plastic boxes with lids (for smaller items) are stored on a special shelf near the lab door. given the chance. except in small quantities needed in the labs. 29 . Observe and respect the No Storage labels in certain areas in the labs. Refills of hazardous chemicals. If your bottle is very small. Proceed with caution and follow the techniques described below. are stored in closed cabinets. which might become involved in a fire. set it inside a beaker in the covered box to prevent tipping & spilling.

 Clean (and report) all spills immediately (see BASIC LAB PRACTICES and SPILL RESPONSE).  Obey signs about designated areas for particular activities.STUDY GUIDE & PRACTICE QUESTIONS TECHNIQUE: To refill a reagent bottle. Practice with a small beaker and water. Return the bottle and the carrier to their proper storage locations. collection the waste in a beaker.  Open & close bottles with care.  Keep a cap or lid in your hand while pouring or set it on a clean watch glass. Any glassware or stir bars that fall into your beaker can be retrieved before you transfer your waste to the appropriate waste bottle. guarding against spills. close the empty container and rinse/wipe the outside of the bottle to remove any contamination.  When a procedure generates a hazardous waste. this guards against contamination of the bench top and the reagent. Request a refill at the Stockroom and carry the refilled container back to your lab in the secondary container. This takes a bit of practice but it allows you to direct the flow of liquid into the receiving vessel. Hazardous materials handling. TECHNIQUE: Dispense liquids from beakers or bottles by holding a clean glass rod to the rim of the pouring container and pouring the liquid down the rod. such as flame tests. Choose the appropriate secondary container and use it to carry the empty bottle to the Stockroom Service Window attendant (in YORK 3150 or NSB 1104). 30 . rinsing your glassware with an appropriate solvent.

 Why are you instructed to transfer methylene chloride only in the fume hood? (Hint: good reasons would include flammability.STUDY GUIDE & PRACTICE QUESTIONS HAZARDOUS MATERIALS Questions:  How can you learn in advance whether a material is hazardous?  In an earthquake.  What is the best way to warn others working in the lab about the hazards of YOUR materials?  Describe a safe method for heating materials over an open flame. all bottles in a storage container may break and mix. machine oil. such as a Bunsen burner. benzene  ammonium nitrate. opium. and potassium permanganate  sodium chloride. explosive peroxides (R-O-O-R) upon air exposure and long-term (months to years) storage?  organic ethers (R–O–R)  inorganic oxides  permanganates  sulfites  saturated organic hydrocarbons (CxH2x+2 )  Which of the following factors affects our choices in storing hazardous materials?  Chemical compatibility  Safety of workers who will handle containers later  Earthquake safety  Convenience  Regulatory mandate  How would you carry an empty 1. ethanol.  Is the difference between hot and room-temperature glass visible? 31 . sodium barbiturate  for o-chem students: What class of chemicals forms shock-sensitive. liquid spills.  Suggest a rule that might be used to prevent tripping on backpacks in a crowded lab. Which of the following is an unsafe group to store together (hint: think about what class of chemical represents)?  hydrochloric acid. sulfuric acid. potassium phosphate  tetrahydrocannabinol.5L bottle to the Stockroom in order request a refill of 1M NaOH solution? Assume NaOH (sodium hydroxide) meets at least one of the criteria for being hazardous. toxicity. calcium sulfate. phosphoric acid  acetone. check the MSDS for methylene chloride (CH2Cl2). volatility.

Describe a storage strategy to avoid such injuries. & Reactivity as well as the appropriate Personal Protective Equipment (e. 32 .  sulfuric acid (H2SO4)  sodium hydroxide (NaOH) pellets  silver nitrate (AgNO3)  ethanol (CH3CH2OH)  acetyl chloride (CH3COCl)  Which of the following materials should ONLY be used in a laboratory fume hood? Why?  Ether  Methylene Chloride  Hydrogen Sulfide  The HMIG (Hazardous Materials Identification Guide) rating on many chemicals in the lab.  Which of the following chemicals is most likely to cause a penetrating burn.STUDY GUIDE & PRACTICE QUESTIONS  Suggest a useful strategy for handling broken or chipped glassware. If a liquid material is rated '4' for flammability. how might this tend to affect your health related exposure?  A common injury in the Teaching Labs is a hand cut from the sharp (narrow) end of a Pasteur pipettes. including unknown samples. Flammability.g. 1 – 2 – 0 – C). 0 is the lowest and 4 is the highest rating. indicates the level of hazard for Health. but little initial pain upon exposure to your skin? Which will cause immediate pain and burning? Which is a significant fire hazard? Choose any that apply..

 2 = moderate. A Material Safety Data Sheet (MSDS) provides hazard information for a specific chemical or product. This system indicates the level of hazard for Health. catalogs. reference books. In other jurisdictions. flash point 100 – 200°F). Prudent practice.  1 = slight. It is expected that our students will enter into workplaces where these regulations apply fully. along with other information sources (see HAZARD COMMUNICATION) can help you evaluate the hazards of the materials you work with. For example: a bottle may be marked HMIG 1 – 2 – 0 – C indicating that the health hazard (including considerations of toxicity.  Businesses that store or handle hazardous materials are required to make information about their materials available to the surrounding community (often via the Fire Department or other local authority). HAZARD COMMUNICATION Clear communication of hazard information allows workers to make informed decisions about the work they plan to do and the materials with which they will work. In the United States Right to Know laws and regulations. This rating. corrosivity.  Manufacturers of hazardous materials are required to supply MSDS when shipping hazardous materials in interstate commerce. along with the materials and the quantities needed in you lab notebook. such as Canada & the European Union. the flammability hazard is moderate (requires moderate heating to ignite. Flammability. however. Students in the Teaching Labs are not employees (although the TAs are) and no similar regulation currently constrains the University to make such MSDS available to students. MSDS are therefore made available to students on the same basis as to employees (Instructors. similar rules apply. Hazardous Materials Identification Guide (HMIG) ratings are shown on many chemicals in the labs. and Material Safety Data Sheets. TAs & staff). suggests that everyone working in an area should work with similar expectations and standards.STUDY GUIDE & PRACTICE QUESTIONS 5. including unknown samples prepared for analysis by students. In each category:  0 = minimal.  3 = serious  4 = extreme hazard.  Employers are required to keep MSDS available to their employees who work with hazardous materials. and other health effects) is slight. Material Safety Data Sheets (MSDS). 33 . Safety data sheets are available in various formats on the internet: search for a chemical name or check the manufacturer’s website. Sources for such information include bottle labels (manufacturer's and our own). Use MSDS to investigate the hazards of the materials you plan to work with and list the hazards. & Reactivity as well as the appropriate Personal Protective Equipment (PPE). lab signs.

the International Agency for Research on Cancer (IARC). that may occur immediately. a PPE rating of C is a recommendation for the use of goggles. Globally Harmonized System. or the National Toxicology Program (NTP). Note that there are a number of items needed by a lab students (melting points. 6. 12. 9. including its chemical and common names. explosive. the Occupational Safety and Health Administration (OSHA) is expected to publish a revision to the federal Hazard Communication Standard (Code of Federal Regulations (CFR) section 1910.1200). Specific fire fighting information. Procedures for cleanup of spills and leaks.STUDY GUIDE & PRACTICE QUESTIONS the reactivity hazard is minimal (normally stable & does not react with water). During 2012. Health hazards. primary routes of entry into the body. 34 .1%). 5. date of issue. You can expect to find the following information: 1. gloves and an apron (or lab coat). Identity of the organization responsible for creating the MSDS. Precautions for safe handling and use. etc. If the material is listed as a carcinogen by the US Occupational. Safety & Health Administration (OSHA). flammable. MSDS ORGANIZATION MSDS are organized according to a set pattern. and will create a new US format for hazard communication. For example:    brand name: Clorox(TM) chemical name: sodium hypochlorite common name: bleach. etc. medical problems that can be aggravated by exposure. including:   Acute effects. Identity of the material. or respiratory disease) that may appear over a period of time or after long exposure. although a variety of formats will be seen.) not normally found in MSDS. 11. Hazardous ingredients (in parts as small as 0. 10. 8. skin problems. 2. reactive. corrosive. Posters in the labs show the details of this labeling system. specific target organs likely to sustain damage. Chronic effects (such as allergic sensitization. Emergency and first aid procedures. Exposure limits. 7. 4. including personal hygiene. Precautions and safety equipment.). boiling points. The standard will incorporate most elements of the United Nation’s Globally Harmonized System of Classification and Labeling of Chemicals (GHS). Physical & chemical hazards and characteristics (unstable. such as burns or unconsciousness. and emergency phone number. 3. The resulting Standard will replace the present system.

If requested.  Save your labels to a file. remove them by cutting down through the center of the label & peeling toward the edges. Health & Safety and the Science & Engineering Library  Paper copies in the Teaching Labs Stockroom (YORK 3150 or NSB1104)  Selected collections in specific labs for the materials used in that lab  General information on using MSDS is available via EH&S . build a table of materials to be used. Once you have the MSDS for a particular chemical. if desired  concentration of solutions: if you only know approximate concentration. Label all containers before filling them Think ahead & plan the labels you will need for your work.STUDY GUIDE & PRACTICE QUESTIONS LOCATE AN MSDS & BUILD YOUR COLLECTION  Bookmark the University of California MSDS search site. In WORD.  Copy the general format from the example NaOH labels below.  Cut the labels apart & tape them to your bottles & flasks with clear tape.  Specify the approximate number of columns & rows needed. When preparing for lab. use that. When no longer needed. give   USERNAME = ucmsds PASSWORD = msds1868  UCSD Environment.  Always include:  your name & date  your room & locker number  the substance to be contained: write full chemical names. 35 . You can add or delete cells later. your Sharpie marker will write on the clear tape. MATERIAL NAME HAZARD QUANTITY PROTECTION/EQUIPMENT NEEDED NEEDED LABEL YOUR MATERIALS Hazard communication includes warning others about the hazards of your materials – letting others know what you’ve learned.  Open the Table menu and choose Insert. Add more information by hand as it becomes available.  hazard information: check the MSDS for this information. It’s often easier to revise a sheet of labels (& save to a new file) than to start from scratch. Including the quantity needed will allow you to think about the possible risk (exposure if spilled). include abbreviations. file it on your computer for easy future reference. try the following.

........... Various manufacturers' catalogs...5M NaOH) CONCENTRATION ... Examples include     Merck Index....  Other web sites. 12.............. 2012 Locker ..... contain a wealth of information................ Health & Safety: 36 ..........  labs staff person assigned to your course....... When seeking either safety information or physical data..... Prepare your labels as part of your lab preparation & label all containers.  Teaching Labs Safety Coordinator.. Room YH3208 CHEMICAL NAME CONCENTRATION ...........STUDY GUIDE & PRACTICE QUESTIONS WRITING LABELS:.... Health & Safety maintains a good list of sites..... such as the Aldrich catalog..... also check:  Bottle labels.......23. Copy this table (into a word processor) and customize it for your needs.  Chemistry Department Safety Director. Pay careful attention to the labels on consumer products available in hardware...... A number of these are available for student use in the Teaching Labs Stockroom (YORK 3150 or NSB 1104). SODIUM HYDROXIDE SOLN (~1..  Reference books.... Name.. Sax’s Dangerous Properties of Industrial Materials is available on-line via the UCSD Libraries reference collection.................. grocery & hobby stores... Some of these products can be extremely hazardous. including the original manufacturer's label and the ones prepared when transferring materials to smaller bottles (secondary containers)........ CRC Handbook of Chemistry & Physics...... Locker ... The UCSD Department of Environment. JOHN PALMER DEC.. Room ...... and  safety professionals at UCSD Department of Environment... seek out:  your TA & Instructor.............. materials that are not sold in interstate commerce are not covered by Federal law and regulations...... For assistance in understanding and interpreting data..... HAZARD CORROSIVE Other sources of information MSDS are not available for all chemicals you can expect to use – or produce – in lab exercises... Date .........

flammable or corrosive (is it TRICky ?). Consider whether each one is toxic. For each material. reactive.STUDY GUIDE & PRACTICE QUESTIONS HAZARD COMMUNICATION Questions:  Where can you locate the MSDS on a material you plan to use in lab?  When is a student permitted to access the Teaching Labs' MSDS files?  Where can you access MSDS files on the web?  When is an employee allowed to use MSDS from the employer's files?  State the responsibilities and rights of the student under the US Federal Hazardous Communication Standard of OSHA  State the responsibilities and rights of the worker under the Hazardous Communication Standard of OSHA  State the responsibilities and rights of the supervisor/employer under the Hazardous Communication Standard of OSHA  Who has a right-to-know? Know what?  Locate and read the MSDS for 4 of the following substances. write a label for a container holding 100 mL of a 0. the degree of flammability. find the molecular formula.  hydrochloric acid  sodium hydroxide  acetone  ethanol  potassium permanganate  diethyl ether  For each of the materials given above. and the reactivity of the material. 37 . the hazards to health.5M solution that you will store for several weeks. if any.

Matching the arrows places the sash in the highest operating position.  Set up equipment at least 6" back from the sash to capture fumes and vapors. adding a small portion of fresh ‘makeup air.  Close the hood sash as much as possible. Our LAB RULES are an example of a set of administrative controls. but close – at least to the arrows – before beginning work.  Open hazardous volatile materials (those that vaporize easily) only in the fume hood. opening the windows just enough to do your work while maintaining proper air flow. Fume hoods & air handling systems carry fumes and vapors away from a worker's breathing space. An office or home ventilation system typically recirculates most of the air (to save energy). They include fume hoods. OPEN ONLY IN HOOD NOTICE: Failure to follow procedures may cause your supervisor to explode.STUDY GUIDE & PRACTICE QUESTIONS 6. Administrative controls are the rules we work under.  Observe the arrows posted on the frame and the window of the hood (see photo above). whether they are rules agreed by a group of co-workers or those imposed by an employer or teacher. ENGINEERING & ADMINISTRATIVE CONTROLS Engineering controls are the built-in protections which distinguish a properly constructed lab space from an ordinary office space. Our labs use 100% fresh air. fire sprinklers. Open further to place large equipment into the hood.  View video presentation (Basic Fume Hood Air Flow and Operation) on our Safety Videos page for a good working demonstration. 38 .’ Our lab system supplies enough fresh air to allow the entire volume of air in the lab to be replaced ten times each hour. and fire walls & doors. air handling systems. Engineering controls provide passive protection – they protect all workers in an area without action on the worker’s part.  Keep lab doors closed as much as possible to allow the ventilation system to work as designed. Others are the signs posted in labs directing (or forbidding) particular actions. as are the BASIC LAB PRACTICES in Chapter 1.

some are recessed into the dropped ceilings. Never try to silence an alarm without finding and fixing the problem. Fire protection. Use another hood until the broken one is fixed. When an alarm sounds. indicating that the problem is fixed. Tape a note on the hood noting when the problem was reported & to whom. Report the alarms to the Lab Staff and delay any work that needs fume hoods. ENGINEERING & ENGINEERING CONTROLS Questions:  What are fume hoods for and how should they be used?  Why are lab workers instructed not to prop open lab doors for fresh air? 39 . Keep all lab doors closed and always close them when leaving. delaying the spread of a fire and protecting both occupants & Emergency Responders. If all alarms in one room are sounding. the walls and fire doors will contain the fire. notify the Lab Staff so the fume hood can be repaired. All our labs have fire sprinklers. allowing evacuation through the adjacent hallway. In a fire. They also allow fire fighters to approach the room and set up their equipment before they are exposed to the fire. suspect a system failure. although not all are obvious. If the alarm continues. fire walls & closed fire doors provide a 1-hour fire envelope. check to make sure the sash is properly positioned – this alone may silence the alarm.STUDY GUIDE & PRACTICE QUESTIONS Fume hood alarms indicate a malfunction in this protective air handling system. In a larger fire. Sprinklers are heat activated and respond one at a time – usually one sprinkler will extinguish a lab fire.

PERSONAL PROTECTIVE EQUIPMENT (PPE) THE MINIMUM LEVEL OF SAFETY PROTECTION NECESSARY TO WORK IN THE TEACHING LABS IS SAFETY EYE PROTECTION. ballet flats and other open shoes are inappropriate. Labs are kept cool so long clothing (trousers and lab coats) is comfortable. Check your decision with your TA until you feel confident in your own decisions. A work plan that involves skin contact with chemicals needs to be revised. Store lab coats in the lab or carry them folded in plastic bags to prevent spreading contamination. If the lab is too warm. Synthetics fibers. LONG PANTS (OR EQUIVALENT). Wearing a lab coat away from the lab area indicates a disregard for the health and safety of the community. AND LONG (KNEE-LENGTH) LAB COAT. 40 . TA and Instructor) takes into account the chemical and physical hazards of lab work – the presence of corrosive and flammable materials as well as the use of open flames. worn or contaminated. tend to burn easily or melt onto skin and are particularly inappropriate for lab wear. such as nylon. A closed shoe made of nonabsorbent material with a tread that will not slip on a wet floor is preferred. wash hands and get new gloves. consult with Lab Staff if you suspect this will be necessary. Restrain shirt and sweater sleeves so they don’t protrude beyond coat sleeves. can be used to protect the hands from incidental contact with common lab chemicals and protect from dirt & abrasions. or contact with moving parts of machines. Base a decision to wear gloves (or not) on the hazard of the materials you will use. Wash lab coats separately from other laundry. remove loose jewelry. Be aware that sturdy boots or safety shoes are standard practice in many workplaces. Flame retardant natural fibers. STUDENTS WHO ARRIVE UNPREPARED OR INAPPROPRIATELY DRESSED WILL BE DISMISSED UNTIL READY TO WORK. please let the staff know. to protect legs from spills and splash. If gloves are dirty. Choose long pants (or equivalent clothing).STUDY GUIDE & PRACTICE QUESTIONS 7. When working in lab. Shoes that are closed all around are required to protect the feet from broken glass and chemical spills. such as cotton. Restrain hair and loose clothing away from flames. Sandals. Request assistance if a safer plan doesn’t seem feasible. staff. CLOSED SHOES. rinse and remove them. Unusually hazardous materials or extended contact will require greater protection. LAB CLOTHING Appropriate clothing for lab workers (students. available in the labs. wool and linen. contamination. LAB GLOVES Light-weight disposable gloves. Wear a long-sleeve. knee-length lab coat closed to protect skin and clothing. are preferred for lab clothing.

Lab workers who forget to remove gloves can carry hazardous materials to clean areas and contaminate others. Chemical splash goggles are required when anyone in a work area is transferring more than a trivial amount of an eye hazardous substance. Safety glasses provide protection from impact (as from broken glass) but little protection from liquids splashed into the face. when the first person begins to work with glassware or chemicals. 41 . The choice of appropriate eye protection (see illustrations) is governed by several factors.Department of Chemistry & Biochemistry. specific procedures with a high level of eye hazard are restricted to "goggles only" areas.STUDY GUIDE & PRACTICE QUESTIONS Always remove gloves & wash hands before leaving the lab or starting a new activity. Note that everyone who might be affected by a spill in the area wears splash goggles until the procedure is completed. In some laboratories. There are cases where additional eye and face protection are warranted and face shields are available for these cases. they are appropriate for dry lab work. TAs. Instructors. Safety eyewear THE DEPARTMENT RULE: Safety eye protection is required for all workers in all labs when anyone is working with glassware or chemicals. Chemical splash goggles (see illustration) fit closely to the face. staff. please remind them. as well as protective clothing. As long as no one in the room is doing lab work. eye protection is not required. they will be supplied with goggles. (Visitors should check in with the Stockroom (YORK 3150 or NSB1104). and visitors) must don appropriate eye protection. everyone (students. especially across the forehead. Goggles purchased for other activities may not be appropriate for chemical work. but such cases rarely occur in student laboratories. Ask the safety staff to be sure. and are vented indirectly to prevent a splash from reaching the eyes. . not goggles. If you see others wearing gloves in the halls or elevators. UCSD This Department Rule allows for the use of a laboratory as a classroom for a lecture or quiz.) GOGGLES -------------SAFETY GLASSES ------------- Visorgogs® SAFETY EYE PROTECTION: NOTE that Visorgogs® are glasses. not just the person carrying or pouring the hazardous substance.

42 .STUDY GUIDE & PRACTICE QUESTIONS GOGGLES ONLY. Anti-fog cleaners (available in the Bookstore and in the Lab Stockrooms) provide some relief from fogging. Goggles are always appropriate. TAs. and Astrospec 3000 series glasses). or when commercial products are mixed. as for any other personal item. In recent terms. dB) provided. Chemistry 7L and 143A have been "goggles only" for all students. check your decision with your TA until you feel confident in your own decisions. The Astro OTG3001 and Ultraspec2001 glasses are designed to fit over prescription glasses. a lab class may (at the option of the Instructor) be designated a "goggles only" class. and visitors in these classroom. friends and co-workers. As consumers with safety training. Excessive noise is not generally a concern in chemistry labs. ENCON series 500) and approved safety glasses (for example. The UCSD Bookstore and carries approved chemical splash goggles (for example. Remember that the hazard of a materials (chemical) or operation doesn’t change when the operation happens at home. Hearing protection. many chemical accidents occur at home. Instructors. Be aware that this is the UCSD CHEM Teaching Labs rule. staff. Wash goggles and glasses frequently. Be aware that hearing protection is standard PPE in many industrial jobs and for any use of power tools (at home or at work). Contact Lenses.  eye protection and hearing protection for target shooting or power tools. For simplicity and for the greatest measure of safety. and  sturdy boots for moving heavy materials. some labs and some employers do not allow contact lenses. Astro OTG 3001. Indeed. Check the class syllabus for current requirements. we expect each of our students will become a valuable resource to their families. when consumers mistakenly assume commercial products are safe. When worn with safety eyewear (goggles or safety glasses). contact lens wear is acceptable. The lab TA and Instructor will assist in developing judgment about such situations. The Teaching Labs Stockroom does not sell or lend goggles or glasses to students. Hearing protection is rated by the amount of noise reduction (in decibels. if unsure about the better option. PPE AT HOME Many household and hobby materials are hazardous. UVEX brand Ultraspec 2000 & 2001. More advanced lab students are expected to have both goggles and glasses and to be able to distinguish the situations in which each is appropriate. but occasions arise when protection is necessary. Examples include:  gloves and goggles for wet chemicals. Use.and encourage other to use – protective equipment appropriate to the activity. Check the MSDS on some household and hobby chemicals and consider whether they are handled correctly in your home or hobby group.

they can be quickly removed. isolating you from harmful exposures or flames.g. melt.  Choose a disposable lab coat when using high-hazard materials or when hazard-appropriate decontamination services are not readily available. shrink and stick to skin. Carry coat in a closed plastic bag. DO . Snap closures are preferred over buttons to allow quick removal in an emergency. coats may be laundered. Do add an impervious apron (rubber or coated fabric) when there is a significant chance of exposure to corrosive materials or when working from a seated position (as in a wheelchair). separate from general laundry and wash frequently. Adapted from materials produced by: Risk Management & Safety 43 . Do choose a lab coat that covers the knees and has full-length sleeves. Properly worn. Although. Do chose a lab coat based on the expected work hazards:  A lab coats made of cotton fabric is recommended for general lab use. follow these DOs and DON’Ts. a lab coat covers your clothes and minimizes the risk of exposure to spills & aerosols. To minimize exposures to harmful substances in the lab and provide some temporary protection against fire. contaminated or on fire. Synthetic fabrics burn. Don’t roll up the sleeves on lab coats or allow shirt/sweater sleeves to protrude.  In high-hazard areas. Don’t wear lab coats outside the lab area or take a contaminated lab coat into a food area. . Don’t wear lab coats unbuttoned. An open coat is an invitation for hazardous exposures. Nomex) if there is a significant fire potential. DON’T . Do immediately remove a lab coat if wet. . .  Choose flame resistant fabric (e. If contaminated with hazardous material. separated from general laundry. choose a coat that has no openings for access to pockets.STUDY GUIDE & PRACTICE QUESTIONS DOs & DON’Ts of Lab Coats . most lab coats are not designed to be impermeable to hazardous substances or flameproof. . Do wear lab coats completely “buttoned” up. . Once decontaminated. . they can compromise the wearer’s safety. Do wear a lab coat at all times when working in a lab.. It also provides short-term protection from saturation by harmful substances and temporary protection against fire. Do keep lab coats clean. Don’t use a lab coat made of synthetic fabric in a fire hazard area. coat will be decontaminated on site or disposed as a hazardous waste. Ask for assistance if you’re hard to fit. For general dirt.

 When should you wear protective gloves in lab?  What might happen to a students who arrives for a lab class in sandals or shorts?  What is the minimum appropriate level of personal protection required in the Teaching Labs?  Does the Teaching Labs Stockroom lend goggles to students who forget? What if it's your birthday?  When are you REQUIRED to wear your safety glasses (or goggles) by departmental policy?  What is the minimum eye protection required when you pour 30 mL of a hazardous liquid or are in danger of being splashed?  What is the appropriate eye protection for a lab worker pouring 2L of boiling water? 44 . Give an example of an activity where each is appropriate. it is recommended you wear:  When are splash goggles required in the Chemistry Teaching Labs?  Is a Lab Supervisor (Instructor or TA) permitted to make a safety rule which is stricter than the Department Rule?  What eye protection is required in your lab class?  Does handling hazardous chemicals at home require protective equipment? Why/why not?  Describe how you will apply the safety knowledge you learned from this course to your work environment or your teaching environment.       True False Safety glasses with side shields Safety goggles A face shield with safety glasses or goggles A face shield  To protect your eyes when mixing strong caustics or acids.  Contact lenses are not recommended for use in the Teaching Laboratories.STUDY GUIDE & PRACTICE QUESTIONS PERSONAL PROTECTIVE EQUIPMENT (PPE) Questions:  Distinguish between safety glasses and splash goggles. give the important similarities and differences.

please let the staff know so it can be replaced. 45 . razor blades and any item with a sharp edge or point. In any lab that uses needles.” assume experimental wastes are hazardous and look for appropriate waste containers. . each will have a UCSD Hazardous Waste Tag. WASTE MANAGEMENT Wastes generated in the labs range from nearly innocuous (towels wet with water) to hazardous (flammable or toxic materials). If the tag is missing or defaced. . This practice minimizes spills. evaporation of volatile wastes. as required by law. Sharps are items that could cut through a plastic trash bag. These items include both broken items and those intended to be sharp:      broken glassware. . especially to draw blood. Waste bottles are typically 4. . Careful disposal of wastes protects the safety of all workers. Capillary tubes and pipettes can be placed in the large broken glass boxes or special disposal containers may be provided. needles. workers receive training in proper disposal. it must be closed by each worker after each addition of waste. Special containers are provided in the labs for the disposal of sharps. In addition. Unless you have specific instructions to “dispose to drains. because we're all using it . except when actually adding waste. Place broken glass and other sharps in the labeled cardboard containers provided. fine glass pipettes. Leaving a waste container open in a hood (". and fines by regulatory agencies. including the essential maintenance staffs who support our lab work. Keep each waste container in a tray (in case of spills or leaks) and keep them closed at all times. Chemical waste containers are provided and labeled by the Lab Staff.STUDY GUIDE & PRACTICE QUESTIONS 8. Needles are seldom used in the Teaching Labs. but present a special public health problem in clinical labs. Hazardous chemical wastes. ") does not meet this rule.to 20-liter bottles with descriptive labels.

You also have three hazardous waste containers labeled: 1. "ORGANIC SOLVENTS ONLY". ask your TA or the Lab Staff member assigned to your lab. "LIQUID WASTE ONLY" 3. If you don't see a container appropriate for your waste. cap it securely and leave it in its tray. and request another bottle for that waste from the Lab Staff. water. your spatula. 46 . and two hazardous wastes: toxic solid you have synthesized. try to find someone who still needs the item. dried & weighed on filter paper and a mixture of liquids including acid.STUDY GUIDE & PRACTICE QUESTIONS If a waste bottle is full. and metal ions. then dispose of any remaining excess in the appropriate waste container. "DRY SOLIDS ONLY" 2. Excess chemicals which will not be used are never returned to the stock bottle (due to the chance of contamination). WASTE MANAGEMENT Questions:  What is the best way to deal with broken glass with a hazardous solid residue?  What should you do with a hazardous waste generated in the Teaching Labs?  Why are you instructed never to return chemicals to stock bottles?  What should you do with excess hazardous solid you will not use?  Devise a disposal strategy: You have your wash bottle of water.

Close and lock doors and post a sign (stocked in the Spill Kits) on the lab doors to avoid reentry by persons unaware of the spill. the vapor pressure will be low and the risk of exposure smaller. Complete an Accident Report and turn it in to the Stockroom. all spills and accidents must be reported to the lab supervisor (TA or Instructor) without delay. consider life before property. HAZARDOUS CHEMICAL SPILL ON SKIN OR EYE.  if other responsibilities (students. See Chapter 3 (EMERGENCY & DISASTER RESPONSE). If the situation is larger or more hazardous than you are prepared to handle. All spills and accidents require Incident Reports. animals. Mercury is a neurotoxin and the primary route of exposure is inhalation of mercury vapor. 47 . TECHNIQUE: Call 858-534-4357 from any phone (landline or cell phone) for any emergency assistance on campus. If mercury is spilled in/on a hot environment (sand bath. Give assistance to any injured persons whenever it is possible to do so without increasing the danger to yourself or others. The vapor pressure of mercury (and the risk of exposure) rises quickly with rising temperature. Do not make a situation worse by involving or contaminating more people. Small hazardous spills may be cleaned using the materials in lab Spill Kits under the direction of the TA or Instructor. Call for assistance:  if you don’t have the materials or equipment to clean the spill. Notify the Lab Staff and EH&S about the nature and extent of the spill. TA. a partner who needs your help) require your attention. plumbing or electrical emergencies. or capacity to respond  if you’re not sure. A mercury (Hg) spill is a special case. etc. For this reason. If the mercury spill is cold or room temperature. evacuate the room and call for professional assistance.  if the situation is beyond your comfort zone: you don’t have training. For spills. The first people available must respond on the scene (student. oven or hot plate). Campus emergency operator will send help for medical emergencies. as for any accident. Avoid injury (or further injury) by keeping people out of spill area. Study the FIRST AID section carefully. evacuate the lab without delay & call for staff assistance. fires. SPILL RESPONSE Spill response requires an immediate assessment of the hazard presented by the spill. Instructor or staff).STUDY GUIDE & PRACTICE QUESTIONS 9. Use the special cleanup equipment provided in the Spill Kits. children. experience. Choose appropriate PPE & follow instruction provided in the Spill Kit to absorb/neutralize spilled materials before collecting it for disposal. Hazardous chemical spills that are large or volatile (evaporating quickly) require evacuation of the lab and professional assistance.

moisten the cleaned area with a wet sponge and rub a pH test paper (available in labs) across the wet surface. Label the bags with contents.  How can you assure that all acid has been cleaned from a spill on the floor?  Who is available to assist you in cleaning a spill of hazardous materials in your lab?  Where is the Spill Kit kept in your lab?  After cleaning a spill. o leave it to dry on the work bench. Request replacement cleanup materials (at Stockroom Service Window. Used cleanup materials. leaving the Spill Kit fully stocked. Seal used/contaminated materials in plastic bags provided in the Spill Kits. After neutralizing the spill. clean again. For a spill that runs off a lab bench. o collect it in a plastic bag and store in the hazardous waste area of the lab. Dispose of all used waste paper (towels and Kimwipes) in trash receptacles.STUDY GUIDE & PRACTICE QUESTIONS Acid or base spill on inert surface (bench and floor). rinse the surface with water. When the area appears to be clean. spills must be THOROUGHLY CLEANED and NEUTRALIZED. If the surface is not neutral. Neutralize base spills with solid citric acid. Rinse broken glass before placing it in the broken glass receptacles. be sure to check inside drawers for additional liquids. o leave it to dry on the work bench. SPILL RESPONSE Questions:  How should you dispose of the sludge created when neutralizing a small acid spill with sodium bicarbonate? o dump it in the trash bin. then dump in trash bin. Paper used to absorb chemicals should be placed in solid hazardous waste containers.     Dilute the spill with water Neutralize acid with solid sodium bicarbonate. TECHNIQUE: To determine whether a corrosive spill has been thoroughly cleaned. hazards. then store in the hazardous waste area of the lab. how should you dispose of the leftover broken glass?  How can you assure the Spill Kit will have what you need in case of a chemical spill?  How big a spill is too big for a student & TA to clean up? 48 . leave them in the lab's hazardous waste collection area. date and your name. test the wet surface with pH test paper and repeat as needed to assure surfaces are neutral. Sodium bicarbonate and citric acid can be found in the lab Spill Kits. YORK 3150 or NSB 1104) and replace the used items. Compare the resulting color of the strip to the guide on the package label. not in the general trash. Notify staff so they can arrange collection & disposal. Regardless of how small.