Bifidobacterium breve Reuter

ATCC Number
Designations

: 15700
: S1 (Variant a)

Source of Isolation Growth Conditions CM149)

: Intestine of infant : ATCC medium1053: Reinforced Clostridial medium (Oxoid Alternate medium 593: Chopped meat medium

Temperature Atmosphere Applications

: 37°C : ***Anaerobic : Produces restriction endonuclease BbeSI

ATCC medium: 593 Chopped meat medium Ground beef (free of fat)........500.0 g Distilled water....................1.0 L N NaOH............................25.0 ml Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix meat, water, and NaOH and bring to a boil while stirring. Cool to room temperature, skim fat off surface, and filter, retaining both meat particles and filtrate. Add sufficient distilled water to filtrate to restore 1 liter original volume. To the filtrate add: Peptone...........................30.0 g Yeast extract......................5.0 g K2HPO4 .............................5.0 g 0.025% Resazurin solution..........4.0 ml Boil, cool and add 0.5 g L-cysteine . HCl; adjust pH to 7.0. Under 97% nitrogen, 3% hydrogen, dispense 7 ml over 1 part meat particles to 4-5 parts fluid per test tube. Cap with butyl rubber stoppers under N2, H2 and autoclave in press for 15 minutes under fast exhaust.

15.8 +/.…………5. Broth Medium Nutrient Broth (BD cat 234000)…………….0 g Peptone……..2. follow formulation below: Nutrient Agar Composition Beef Extract…………………….0 g Final pH 6. ....…. *This product is commercially available from BD.3.23 g DI Water………………………………….0 g DI Water………………………………….0 g Agar………………………………………….……1000 ml Autoclave at 121ºC.………….Citrobacter freundii (Braak) Werkman and Gillen ATCC Number Designations : 8090 : [ATCC 13316.1000 ml Autoclave at 121ºC.8.0. To make medium from scratch.. *Omit agar for broth medium. NCTC 9750] Growth Conditions Temperature Atmosphere Applications : ATCC medium3: : 37°C : Aerobic Nutrient agar or nutrient broth : produces restriction endonuclease CfrAI quality control strain reference material quality control strain for Sensititre products ATCC Medium: 3 Nutrient Agar/Broth Agar Medium Nutrient Agar (BD 213000)………………….…………………….

BBL bioMerieux Vitek. Gently mix and dispense as required..950 ml Autoclave at 121ºC..950 ml Follow same procedure as agar medium above. Broth Medium Tryptic Soy Broth (BD 211825)……………..0 g Sheep Blood (defibrinated)…………………50 ml DI Water…………………………………. IDS.30. Micro-Media.50 ml DI Water……………………………………. If commercialized product is not available. Tryptic Soy Agar can be made from scratch. MicroScan and Sensititre products ATCC Medium: 260 Tryptic Soy Agar/Broth with 5% Sheep Blood (defibrinated) Agar Medium Tryptic Soy Agar (BD 236950)……………40 g Sheep Blood (defibrinated)………………. by following the formulation below: .. Cool sterilized medium to ~47ºC..….Enterococcus faecalis (Andrewes and Horder) and Schleifer andKilpper-Balz deposited as Streptococcus faecalis Andrewes and Horder ATCC Number Designations : 29212 : [Portland] Source of Isolation Growth Conditions sheep blood heart infusion Temperature Atmosphere Applications : Urine : ATCC medium260: Trypticase soy agar with defibrinated Alternate medium 44: Brain heart infusion agar or brain : 37°C : Aerobic : control strain [21528] [21531] evaluation of Mueller-Hinton agar [21524] media testing [21529] [92284] [92325] [92390] [92429] [92819] [92844] [92845] quality control [92216] quality control strain [92138] [92149] [92159] [92160] susceptibility disc testing [21523] [92224] [92355] susceptibility testing [21525] [21533] [90283] [92217] [92353] [92422] [92423] [92424] [92425] [92788] quality control strain for API. Aseptically add 50 ml of room temperature defibrinated sheep blood.

To make Tryptic Soy Broth from scratch.15.2 *Omit agar for broth medium .. follow formulation below: Brain Heart Infusion Composition Calf Brains.1000 ml Autoclave at 121ºC.. infusion from………………………………….……….……….3 +/.………5..0g DI Water……………………………………………………. *This product is commercially available from BD. To make medium from scratch. Soytone-Papaic Digest of Soybean Meal).………………………………1000 ml Autoclave at 121ºC.2.……..0 g Dextrose…………………………………………….52 g DI Water……………………..Tryptic Soy Agar Composition Tryptone…………………………15..0 g Na2HPO4…………………………………………. Broth Medium Brain Heart Infusion Broth (BD 237500)…………. (Tryptone-Pancreatic Digest of Casein.2.200 g Beef Hearts.………10.5.950 ml pH to 7..0 g NaCl……………………………. follow Tryptic Soy Agar formulation but omit agar.0 g Agar……………………………..0..5..0 g NaCl…………………………………………………...0..37. ATCC Medium: 44 Brain Heart Infusion Agar/Broth Agar Medium Brain Heart Infusion Agar (BD 211065)…………………...4 +/.0 g Soytone………………………….250 g Proteose Peptone………………………………….. infusion from…………………………………..1000 ml Final pH 7.5 g DI Water……………………………………………………….0 g DI Water………………………….2...

55. VPI 6033] Source of Isolation Growth Conditions Temperature : feces : ATCC medium416: Lactobacilli MRS broth : 37.0 g Sodium Acetate……………………………………..0 g Dextrose…………………………………………….0 g Sorbitan Monooleate……………………………….0.5 +/.0 g Yeast Extract……………………………………….0 g DI Water……………………………. deposited as Lactobacillus casei (Orla-Jensen) Holland .2.5.0 g Ammonium Citrate………………………………….0°C ATCC Medium: 416 Lactobacilli MRS Broth Lactobacilli MRS Broth (BD 288130)…………….5..….0 g Beef Extract………………………………………….0 g MnSO4 x H2O……………………………………….0. *This product is commercially available from BD. follow formulation below: Lactobacilli MRS Broth Composition Proteose Peptone #3………………………………. To make medium from scratch.………….05 g Na2HPO4……………………………………………2.1000 ml Autoclave at 121ºC.1.10.Lactobacillus gasseri Lauer and Kandler deposited as Lactobacillus acidophilus (Moro) Hansen and Mocquot ATCC Number Designations : 19992 : 1SL4 [F 164..10.0 g DI Water…………………………………………….2 1000 ml Lactobacillus rhamnosus (Hansen) Collins et al. Final pH 6..20....

55.1000 ml Autoclave at 121ºC. P.E...10.0 g Beef Extract………………………………………….P.10.2. NCTC 6375. NRC 488.0 g MnSO4 x H2O……………………………………….5. Sharpe H2.ATCC Number : 7469 Growth Conditions Designations NCDO 243.05 g Na2HPO4……………………………………………2. M. M.0 g Sorbitan Monooleate……………………………….0 g Sodium Acetate……………………………………. follow formulation below: Lactobacilli MRS Broth Composition Proteose Peptone #3……………………………….0 g DI Water…………………………….5 +/.0 g DI Water……………………………………………...…………. Tittsler 300] Temperature Atmosphere Applications : 37°C : ***5% CO2 : assay of folic acid assay of niacinamide nicotinamide assay of riboflavin vitamin B2 produces folate transport protein ATCC Medium: 416 Lactobacilli MRS Broth Lactobacilli MRS Broth (BD 288130)……………. R. NCIB 8010. Rogosa V300.A. *This product is commercially available from BD.0 g Yeast Extract……………………………………….0.1. To make medium from scratch. .. NCIB 6375.5.20.….2 Carnobacterium funditum Franzmann et al.0...1000 ml Final pH 6.0 g Dextrose…………………………………………….0 g Ammonium Citrate…………………………………. : ATCC medium416: Lactobacilli MRS broth : [BUCSAV 227. Hansen 300.

...0 g MgSO4 ......... CNCTC 28/89.............3..34 g CaCl2 ..................3........... Streptococcus salivarius subsp.......0 g MgCl2 ..... Pf3] : Ace Lake.........0.......13.......... Antarctica : ATCC medium1823: TSBY salt medium : 20....... 21957............14 g Distilled water......................... CIP 102303. 2H2O .................ATCC Number Designations Source of Isolation Growth Conditions Temperature : 49836 : ACAM 312 [DSM 5970.... LMG 6896] Source of Isolation Growth Conditions infusion Temperature Atmosphere Applications : Pasteurized milk : ATCC medium260: Trypticase soy agar with defibrinated sheep blood Alternate medium 44: Brain heart infusion agar or brain heart : 37°C : Aerobic : Produces aminopeptidase N Quality control strain ATCC Medium: 260 Tryptic Soy Agar/Broth with 5% Sheep Blood (defibrinated) ..25 g NaCl..1..... 6H2O ..... B of R....30.......45 g KCl... DSM 20617.........0°C ATCC medium: 1823 TSBY salt medium Trypticase Soy Broth (BD 211768)........4.. 7H2O .. thermophilus (Orla-Jensen) Farrow and Collins deposited as Streptococcus thermophilus Orla-Jensen ATCC Number Designations CCUG : 19258 : NCDO 573 [NCIMB 8510..........0 g NH4Cl ..0............................. Vestfold Hills.......2..0 L Adjust for final pH 7...........0 g Yeast extract.......0...... Autoclave at 121C for 15 minutes...

Cool sterilized medium to ~47ºC.…. (Tryptone-Pancreatic Digest of Casein.0.15.0 g NaCl…………………………….2. by following the formulation below: Tryptic Soy Agar Composition Tryptone…………………………15.200 g Beef Hearts. Aseptically add 50 ml of room temperature defibrinated sheep blood.950 ml pH to 7.250 g Proteose Peptone…………………………………. infusion from………………………………….50 ml DI Water……………………………………..0 g Agar…………………………….5.... To make Tryptic Soy Broth from scratch.0g DI Water……………………………………………………. infusion from………………………………….37.1000 ml Final pH 7. If commercialized product is not available.0.1000 ml Autoclave at 121ºC..………………………………1000 ml Autoclave at 121ºC.Agar Medium Tryptic Soy Agar (BD 236950)……………40 g Sheep Blood (defibrinated)……………….. To make medium from scratch.0 g Dextrose……………………………………………..0 g Sheep Blood (defibrinated)…………………50 ml DI Water………………………………….2 ..………5. Tryptic Soy Agar can be made from scratch.....……….950 ml Autoclave at 121ºC...0 g Na2HPO4………………………………………….0 g DI Water………………………….2.30.5. follow Tryptic Soy Agar formulation but omit agar ATCC Medium: 44 Brain Heart Infusion Agar/Broth Agar Medium Brain Heart Infusion Agar (BD 211065)…………………. Gently mix and dispense as required..950 ml Follow same procedure as agar medium above..…….0 g Soytone…………………………..0 g NaCl…………………………………………………. Broth Medium Tryptic Soy Broth (BD 211825)……………..4 +/.. *This product is commercially available from BD.………10...2..52 g DI Water…………………….……….3 +/.. Broth Medium Brain Heart Infusion Broth (BD 237500)…………. follow formulation below: Brain Heart Infusion Composition Calf Brains.5 g DI Water………………………………………………………. Soytone-Papaic Digest of Soybean Meal)..

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