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H PLC anal si y s

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Chemical Forums > Chemistry Forums for Students > Organic Chemistry Forum > HPLC analysis

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Topic: HPLC analysis (Read 4551 times)

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christina

Regular Member Mole Snacks: +4/-3
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HPLC analysis

« on: November 03, 2007, 07:18:43 AM »

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How do I analyze a HPLC result? The lab tech injected the sample into the machine and I have the result paper for the sample injected which was caraway oil. However I'm not sure what to do with this now. It was recorded in volts per minutes. Thank You
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« Reply #1 on: November 03, 2007, 10:07:21 AM »

Posts: 27

Analysis of chromatographic results can depend on what you want or need to know. At its most basic, chromatography is a method used to separate different chemical species from each other. The resulting chromatographic trace can thus indicate how pure your sample is by the number and height of peaks present. Pure samples will generally only have one peak. Without integration of peak areas, most of the information you can glean from a trace will be qualitative. Another bit of information that you can take from your sample trace will be the retention time of your product and the relative chromatographic parameters such as column type and size, solvent composition, flow rate, and even temperature. If you have a chromatogram of a standardized sample of your product, you could compare retention times. With a set of standard chromatograms at different concentrations, you could generate a calibration curve with the integrated peak heights, and determine exactly how much of your product the
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w w chem i al or um s. com / i dex. php?t opi =20373. 0 w. c f n c

php?t opi =20373. Logged Ads by Google HPLC HPLC LC MS HPLC Grade Solvents Trace Gas Analysis christina Regular Member Mole Snacks: +4/-3 O ffline « Reply #2 on: November 03. 0 w. com / i dex. 10:24:12 AM » Re: HPLC analysis Gender: Posts: 49 Thanks but all I was given was a drawing on a board which was what the result should look like without numbers.com Applied Separations has a full line of flash columns & accessories Chemical Forums > Chemistry Forums for Students > Organic Chemistry Forum > HPLC analysis Jump to: => Organic Chemistry Forum go Mitch Andre Garcia's Chemical Forums 2003-Present. 2007.appliedseparations. c f n c 2/ 2 .1/ 11/ 13 H PLC anal si y s determine exactly how much of your product the TA injected into the machine.097 seconds with 21 queries. it just depends on what you need to know. Simple Machines XHTML RSS WAP2 Page created in 0.3 | SMF © 2011.0. There is a lot of available information in a chromatogram. w w chem i al or um s. However since my sample only had one large peak I assume it was pretty pure after distilling the sample of caraway. Logged Pages: [1] Go Up PRINT « previous next » Flash Chromatography www. It was just a plain sketch of the line so I guess I just have to say whether there was contamination or not. Powered by SMF 2.

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