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No . 1.

Topic Identification and biological activity of potential probiotic bacterium isolated from the stomach mucus of breast-fed lamb

Author H. Kiňová Sepová, *M. Dubničková; A. Bilková; M. Bukovský; L. Bezáková

Abstrak The lactic acid bacterium E isolated from the stomach mucus of breast-fed lamb was identified by sequencing of 16S rDNA fragment and species-specific PCR as Lactobacillus reuteri. Its potential antimicrobial activity and ability to modulate immune system in vitro and in vivo was determined. The growth inhibition of potential pathogens decreased from Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella enterica ser. Minnesota to Escherichia coli. The lowest inhibition activity was observed in the case of Candida albicans. The ability of L. reuteri E to modulate biological activities of human and mouse mononuclear cells was estimated in vitro and in vivo, respectively. The production of IL-1β by monocytes in vitro was significantly induced by L. reuteri E (relative activity 2.47). The ability to modulate biological activities of mononuclear cells by living L. reuteri E cells in vitro in comparison to disintegrated L. reuteri E cells in vivo differed. For example lysozyme activity in vitro was inhibited while in vivo was stimulated (relative activities 0.30 and 1.83, respectively). The peroxidase activity in vitro was stimulated while in vivo was inhibited (relative activities 1.53 and 0.17, respectively). Obtained results indicate that L. reuteri E is potential candidate to be used in probiotic preparations for animals and/or human.


Biological activity of phthalated endotoxin

Thomas G. Pistole

Glycolipid (GL) was extracted from a heptoseless mutant of Salmonella minnesota by a mixture of phenol,

but which also exhibits a loss of ability to render rabbits tolerant to challenge with untreated GL. 3. Rabbits immunized with any of the three GL preparations exhibited dermal toxicity responses comparable with those in untreated animals. rabbits pretreated with either of the modified preparations exhibited a reduced pyrogenic response to a subsequent challenge dose of the homologous material but no reduction when challenged with the parent GL. a portion of the GL was untreated. The GL was subjected to treatment with either acetic anhydride or phthalic anhydride. to the modified preparations. Aktivitas Biologi Enam Jenis Ekstrak Tumbuhan Famili Asteraceae terhadap Larva Ratna Sari Dewi dan Biological Activity of Six Plan Extract from Asteraceae on Spodoptera litura Fabricius (lipedoptera : Noctuidae) . Both of the chemically treated preparations as well as the parent GL were examined for biological activity in the following systems: mouse lethality assays. rabbit pyrogenicity assays. and petroleum ether. The results of these studies indicated that both treated preparations were less toxic in mice than the parent GL.chloroform. it was concluded that treating GL with either phthalic anhydride or acetic anhydride results in a product which is less toxic in mice and less pyrogenic in rabbits than the parent GL. Compared with saline-treated controls. Based on these findings. and rabbit skin assays. Pretreatment with the unaltered GL rendered rabbits tolerant to the homologous material and. to some degree.

Spodoptera litura Fabricius (Lepidoptera : Noctuidae) Dadang Larvae.9 days.9. and 94. Plant extract were obtained by meseration method using menthanol. respectively Extract of H. Eric Deharo1. The extract were bioassayed to the second instar larvaae of S. scaber prologed the depelopment of larvae by 4. Gynura procumbens (leaf) Artemisia vulgaris (leaf) Soncbus arvensis (leaf) and Helianthus annus (seed) were use in this study. Valérie Jullian1. respectively.2†. litura to evaluate the mortality. antifeedant and growth regulation activity. Elephantopus scaber (leaf). Séverine Chevalley1. little is known about its potential as an anti-plasmodial . Six species. annus caused mortality of larvae by 86% at 5%. annus. 4.7% and 81. and E. Biological activities of nitidine. The aim of this study is to explore other asteraceae species in other to search for insecticidal activity to Spodoptera litura Fabricius (Lepidoptera: Noctuidae). Crysanthenum cineariaefolium.2† Background Nitidine is thought to be the main active ingredient in several traditional antimalarial remedies used in different parts of the world. 4. scaber have high antifeedant activity at 5 % by reducing larval feeding 87. Blumea balsamifera (leaf). A.2. and Agerantum houstoneanum. Extract of B.1% in choice test method.1% and 86. The widespread use of these therapies stresses the importance of studying this molecule in the context of malaria control. balsamifera and E. Asteraceae is one of plant family that is known to have insecticidal activity to several insect pests.1. 3. While extract of H. such as Parthenium argentatum.2. However. a potential antimalarial lead compound Jérome Bouquet.8% in no choice test. Marion Rivaud1. vulgaris.

but rather in the cytoplasm of the parasite.2*† drug. its moment of action on the parasite cell cycle was studied. The in vitro abilities of nitidine to bind haem and to inhibit β-haematin formation were also demonstrated. Nitidine was able to form a 1-1 complex with haem in vitro and also inhibited βhaematin formation with the same potency as chloroquine. . this was consistent with the observation that nitidine did not localize in the nucleus.and Alexis Valentin1. The nitidine's selectivity index compared with cancerous and non-cancerous cell lines was then determined. the anti-malarial potential of nitidine was evaluated in vitro on CQsensitive and -resistant strains. Methods In this study. Nitidine's moment of action on the parasite cycle showed that it could not interfere with DNA replication. To gain information about nitidine's possible mode of action. no sign of acute toxicity was observed during treatment. and also a satisfying selectivity index (> 10) when compared with a non-cancerous cells line. using the four-day Peter's test methodology. however. and its localization inside the parasite was determined using confocal microscopy. Results Nitidine showed similar in vitro activity in CQ-sensitive and resistant strains. as well as its mechanism of action. Its in vivo activity was moderate. In vivo assays were then performed.

the antimicrobial and antioxidant activities of essential oil . The antimalarial activity of nitidine could therefore be improved by structural modification of this molecule to increase its penetration of the digestive vacuole in the parasite. This detailed study of the anti-plasmodial activity of nitidine showed that this known antiplasmodial molecule. Nitidine was active in vitro on chloroquine-sensitive and -resistant Plasmodium strains and displayed an adequate selectivity index when tested on a non-cancerous mammalian cell line. Its ability to bind haem and inhibit βhaematin formation in vitro suggests a possible chloroquine-like mechanism of action and paves the way for further structural optimization. from Algeria Amel Bendiabdella h1. which is present in several traditional anti-malarial remedies. Biological activities and volatile constituents of Daucus muricatus L. In vivo evaluation of nitidine showed that it had moderate anti-malarial activity and displayed no sign of acute toxicity. Its ability to complex haem and inhibit β-haematin formation suggests a mechanism of action similar to that of chloroquine. 5. where haemoglobin metabolization takes place. Mohammed El Amine Background In order to find new bioactive natural products.Conclusion Nitidine can be considered a potential anti-malarial lead compound. can be considered an interesting lead compound.

The chemical composition of the volatile fraction isolated from different organs of Daucus muricatus were studied by HS–SPME/GC–RI and GC–MS after optimization of Solid Phase MicroExtraction parameters. Hocine Allali1.3– 72. Results The chemical composition of essential oils obtained by hydrodistillation (HD) was investigated using Gas Chromatography–Retention Indices (GCRI) and GC–Mass Spectrometry (GCMS). Two types of essential oils were produced by D. flowers. For all organs studied.7–82. stems. The oil from roots revealed active against S.. and umbels) was mainly composed by terpenic hydrocarbon compounds (62. muricatus: (i) The oil from roots is mainly composed by nonterpenic oxygenated compounds (59. Only quantitative differences between the volatiles of the separated organs studied were observed. Conclusions Daucus muricatus essential oil seems be a . the leaves. the activity of the oil of D.Dib1.e.8 g/100 g). aureus.2 g/100 g). albicans. Nassim Djabou1.2. the main volatiles emitted by the plant were hydrocarbon compounds (60. while the essential oil obtained from the aerial parts was active against the yeast C. and (ii) the aerial part oils (i. Alain Muselli2* and Jean Costa2 components extracted from the separated organs of the Algerian medicinal and aromatic plant Daucus muricatus L. were studied. muricatus against eight bacterial strains and one yeast was investigated. In addition.2 g/100 g). Boufeldja Tabti1.

Bursaphelenchus xylophilus. eugenol. and Sang-Chul Shin Commercial plant essential oils from 26 plant species were tested for their nematicidal activities against the pinewood nematode. The essential oils described herein merit further study as potential nematicides against the pinewood nematode. 7. LC50 values of geranial.120. Allspice (Pimenta dioica) and Litsea (Litsea cubeba) Essential Oils Against Pine Wood Nematode (Bursaphelenchus Xylophilus) Il-Kwon Park. methyl isoeugenol. Propolis digunakan oleh lebah sebagai “lem”. anti-ulcer dan anti-tumor. Mahmoud Lotfy Propolis adalah produk alami turunan dari getah/resin tumbuhan yang dikumpulkan oleh lebah. 6. Good nematicidal activity against B.210. Telah banyak dikenal propolis memiliki kasiat sebagai anti-mikroba. 0.promising source of natural products with potential antimicrobial activity. .480. untuk penambalanpenambalan dan perekatan-perekatan sebagai sarana pertahanan untuk sarang lebah. xylophilus was achieved with essential oils of ajowan (Trachyspermum ammi). respectively. methyl eugenol and neral against pine wood nematodes were 0. 6 and 16 major compounds from ajowan. 0. SangGil Lee. These compounds from three plant essential oils were tested individually for their nematicidal activities against the pinewood nematode. Nematicidal Activity of Plant Essential Oils and Components From Ajowan (Trachyspermum ammi). respectively. isoeugenol. Propolis sudah digunakan dalam pengobatan tradisional selama berabadabad. allspice and litsea oils. antioksidatif.200. Junheon Kim. 0. AKTIVITAS BIOLOGI PROPOLIS LEBAH MADU DALAM KESEHATAN DAN PENYAKIT Dr.517 and 0. 0. allspice (Pimenta dioica) and litsea (Litsea cubeba). Analysis by gas chromatography-mass spectrometry led to identification of 12.525 mg/ml.

asam amino. sequiterpene quinines. Lebih dari 300 senyawa seperti polyphenols. phenolic aldehydes. .Oleh karena itu akhir-akhir ini propolis memperoleh perhatian besar sebagai bahan yang sangat potensial dapat digunakan untuk dunia kesehatan dan kosmetika. Komposisi kimia propolis adalah sangat kompleks. steroid dan senyawa-senyawa inorganic telah teridentifikasi dalam propolis. coumarins. Selanjutnya. waktu dan jenis tanaman sumber. Konsekuensinya. aktifitas biologi propolis yang dikumpulkan dari area phytogeographical yang berbeda dan waktu yang berbeda akan sangat berbeda pula tingkat aktivitasnya. propolis sekarang juga dugunakan secara ekstensif sebagai suplemen makanan dan bahan penyegar (beverages) dengan klaim dapat menjaga atau meningkatkan kesehatan manusia. Kandungan bahan-bahan tersebut sangat bergantung pada lokasi pengimpulan. Dalam review ini aktifitas biologi propolis yang disampaikan diutamakan pada aktivitasnya sebagai anti-tumor.