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Technical Bulletin

The EX-CELL® CHO cGMP Library: A Diverse


Collection of Off-the-Shelf Media Designed
Specifically for the Biopharmaceutical Industry
Introduction Components
Chinese Hamster Ovary (CHO) cells are of great interest for
bioprocess and pharmaceutical research and development. Nine serum-free, regulatory friendly catalog formulations
These cells are robust in culture and are able to produce a available off the shelf:
variety of recombinant glycoproteins at high levels and on a • 14360C (LM)/24360C (DPM): EX-CELL® CD CHO Serum-
large scale. However, different CHO cell clones often possess Free Medium for CHO Cells, Chemically Defined, without
diverse nutritional requirements. As a result, media L-glutamine, without hypoxanthine, without thymidine.
optimization for CHO cells can be very challenging, often • 14361C (LM)/24361C (DPM): EX-CELL® CD CHO Serum-
requiring the development of a custom media for each Free Medium for CHO Cells, Chemically Defined, without
particular clone. L-glutamine, with hypoxanthine, with thymidine.
• C1490 (LM): EX-CELL® CD CHO-3 Medium, Chemically
The traditional catalog offer of CHO media is designed Defined
around one or two formulations that meet the regulatory
• C4726 (LM): EX-CELL® CD CHO-2 Medium
description and process functionality of most customers. This
process provides customers a starting point; however, it does • C5467 (LM)/C9098 (DPM): EX-CELL® ACF CHO Medium,
little to reduce the time to market for most process without L-glutamine
development scientists. • C8862 (LM): EX-CELL® ACF CHO DHFR- Medium, without
L-glutamine
The SAFC BiosciencesTM CHO cGMP Library is a diverse • 14340C (LM)/24340C (DPM): EX-CELL® 325 PF CHO Serum-
collection of off-the-shelf media designed specifically for the Free Medium for CHO Cells, Protein-Free, without
industrial biopharmaceutical industry. This collection of media L-glutamine
allows a consistent starting point for new media screening • 14326C (LM)/24326C (DPM): EX-CELL® 302 Serum-Free
projects. The library was developed through screening a Medium for CHO Cells, without L-glutamine, without phenol
battery of formulations against several different cell lines. Each red, without sodium bicarbonate
of the formulations found within the library were chosen for • C6366 (LM): EX-CELL® CHO Cloning Medium
their ability to meet the divergent needs of numerous cell
lines, while also meeting the scale-up needs of process 12+ additional serum-free formulations produced cGMP are
development scientists. The majority of these formulations available off-the-shelf for responsive media screening needs:
have a proven track record in industrial applications and data Formulations are constantly added and optimized in the
shows that, with or without adaptation, several formulations library. Contact Technical Services for the most up-to-date
can be quickly selected for further investigation. option.
• 9+ Animal-Component Free & Chemically Defined
It is recommended that many formulations be tested to truly
identify the unique nutritional requirements of each clone. • 3+ Animal-Component Free
However, time and resources may only allow for a finite
screening project. Thus, a list of formulations can quickly be
tailored based on each customer's application.

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SAFC Biosciences, Inc. SAFC Biosciences Ltd. SAFC Biosciences Pty. Ltd.
13804 W. 107th Street Smeaton Road, West Portway 18-20 Export Drive
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USA
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Formulation Methods for Use
The formulation may include inorganic salts, HEPES, sodium Adaptation
bicarbonate, essential and non-essential amino acids, NOTE: Clones utilizing the GS selection system may require
vitamins, recombinant human insulin, trace elements and custom protocols. Please call our Technical Services
other organic compounds. Some formulations may contain department or e-mail us at technicalservices@sial.com.
plant-based protein hydrolysates. The formulations do not
contain L-glutamine, antibiotics or antimycotics. Some
formulations do not contain purines or pyrimidines to provide EX-CELL® CHO Serum-Free Media
an appropriate medium for specialized CHO cell lines (i.e., Mammalian cells can be adapted to serum-free conditions by
Glutamine Synthetase, or the GS SystemTM, and DHFR- direct adaptation from serum-containing media or by gradual
selection systems). weaning. Both procedures require healthy, viable cultures in
mid-logarithmic growth phase. During the adaptation phase
growth rates will usually be somewhat slower than growth in
For applications that do not require the selective pressure media supplemented with serum.
of a hypoxanthine/thymidine (HT)-deficient medium, we
recommend the use of an EX-CELL® SF CHO formulation that
contains hypoxanthine and thymidine. Gradual Weaning to Serum-Free Media (Recommended):
1. Passage cells from the serum-supplemented medium
The formulations for the library offerings are proprietary to directly into EX-CELL® CHO Serum-Free Medium + 5%
SAFC Biosciences. For additional information, please call gamma irradiated Fetal Bovine Serum (FBS) (Catalog No.
our Technical Services department or e-mail us at 12107C) using normal seeding densities.
technicalservices@sial.com. 2. Once confluent (80 - 90%), rinse with 20 mL of Dulbecco's
Phosphate Buffered Saline (DPBS Modified) (Catalog No.
Precautions 59321C) and trypsinize with 5 mL of trypsin. Add 15 mL
of EX-CELL® CHO medium + 2% FBS. Obtain a cell count
Use aseptic technique when handling or supplementing this and pellet the cells by centrifugation.
media. These products are for research or for further
manufacturing use. THESE PRODUCTS ARE NOT INTENDED 3. Passage cells into a 75 cm 2 flask containing 30 mL
FOR HUMAN OR THERAPEUTIC USE. EX-CELL® CHO medium + 2% FBS at a density of 1 x 105
cells/mL.
4. Allow cells to reach 80 - 90% confluency. Retain any
Storage
detached cells. Observe viability of detached cells. If
Store liquid medium at 2 to 8 C, protected from light. Do not detached cells are not viable, discard. If viable, reserve
use after the expiration date. and combine with trypsinized cells..
5. Trypsinize cells as in Step 2, reducing the amount of trypsin
Indications of Deterioration to 2 mL. Inactivate trypsin with EX-CELL® CHO medium
Medium should be clear and free of particulates and + 1% FBS. Centrifuge.
flocculent material. Do not use if liquid medium is cloudy or 6. Resuspend cells in EX-CELL® CHO medium + 1% FBS at
contains precipitate. Other evidence of deterioration may a density of 2 x 105 cells/mL.
include color change, pH shift or degradation of physical or
performance characteristics. 7. Observe cells daily. Gently rap the flask against the palm
of the hand to detach cells, return to the incubator until
cells reach 80 - 90% confluency (~ 4 - 5 days).
Preparation Instructions 8. Once confluent, retain detached cells and rap flask 2 - 3
EX-CELL® CHO serum-free media are supplied as a sterile 1X times on the palm of the hand or a padded surface. Avoid
liquid and are formulated without L-glutamine. For applications causing bubbles. Triturate cells to further remove cells
requiring the use of L-glutamine, supplement with 4 - 8 mM from the flask and to break up clumping cells. Obtain a
L-glutamine by adding 20 - 40 mL/L of a 200 mM solution cell count and pellet the cells by centrifugation.
(Catalog No. 59202C or G7513). Optimal L-glutamine
concentrations should be determined by the researcher for 9. Resuspend cells at a density of 8 x 105 cells/mL in 75 cm2
each application and cell type. SAFC Biosciences recommends flasks containing EX-CELL® CHO medium without serum
L-glutamine supplementation of the working volume only. until cells reach a density of 1.5-2 x 10 6 cells/mL
(~ 3 - 4 days).
Supplements, such as antibiotics, can be added to the sterilized 10. Triturate cells to break up clumps. Obtain a cell count
medium using aseptic technique. Storage conditions and shelf and pellet the cells by centrifugation.
life of the supplemented product may be affected by the nature 11. Resuspend in EX-CELL® CHO medium at a density of
of the supplement. 8 x 105 cells/mL. Incubate until cells reach a density of
1.5-2 x 106 cells/mL. Take note of cell clump size (number
of cells/clump).

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12. Passage cells as outlined in Steps 9 - 11. 1. Subculture actively growing cells by planting new cultures
13. Repeat Step 12 except seed 250 mL shaker flasks at at 3-5 x 105 viable cells/mL into a mixture of the previous
6 x 105 cells/mL in 60 mL at 60 rpm. growth medium (serum-containing or serum-free "control"
medium) and EX-CELL® PF CHO medium at a ratio of 3:1.
14. After the cultures have been established in EX-CELL® CHO
medium, passage the cells for an additional 2 passages in 2. After 2 - 4 days, count cells and compare cell number
250 mL shaker flasks. and viability to the control cell system. Dilute cell culture
with fresh EX-CELL® PF CHO medium to obtain the same
15. Cells can now be scaled up as necessary using standard seeding density outlined in Step 1.
procedures and densities.
3. If cell growth has been maintained at rates equivalent to
those observed in the control cultures, continue the dilution
NOTES: Adjust protocol as necessary to ensure proper process as described in Step 2.
adaptation to shakers. Start with shaker speed of 60 rpm 4. Higher cell seeding densities may be required for the first
and adjust over the next few passes. few passages in EX-CELL® PF CHO medium alone. Once
If cell clumping is extensive, select for single cells by letting cells are fully adapted to EX-CELL® PF CHO medium,
the large clumps settle to the bottom of the flask and seeding densities can be adjusted to lower densities for
pipetting the single cells for passage. Be sure to count initiating new cultures.
total cells and then count the retained single cell suspension
to ensure proper seed density. EX-CELL® CHO Chemically Defined Media
Always centrifuge cells when passing during the adaptation. Adaptation from Attachment Cultures
Culture density should reach 2-3 x 10 6 cells/mL over 1. Subculture the cells from serum-supplemented medium
3 - 4 days. to EX-CELL ® CD CHO supplemented with 8 mM
L-glutamine using standard trypsinization techniques when
Direct Adaptation to Serum-Free Media cultures reach 100% confluence.
1. Passage the cells into pre-warmed (37 C) EX-CELL® CHO 2. Inactivate the trypsin with medium containing 5% gamma
medium at 1.5-2X the recommended seeding density irradiated Fetal Bovine Serum (FBS) (Catalog No. 12107C).
(2-5 x 105 cells/mL). Using low-speed centrifugation, pellet the cell suspension
at 200 g for 5 minutes and carefully decant the supernatant
2. Refeed the culture after 48 hours with a 100% exchange without disturbing the cell pellet.
of fresh EX-CELL® CHO medium.
3. Resuspend the cells in EX-CELL ® CD CHO medium
3. Allow the cultures to achieve a minimum density of supplemented with 8 mM L-glutamine at a density of
1 x 106 cells/mL before subculturing. 5 x 105 cells/mL in 25 cm2 or 75 cm2 flasks.
4. Subculture into fresh EX-CELL® CHO medium as in Step 4. Incubate the cells at 37 C in a humidified incubator with
1 using normal seed densities. 8 - 10% CO2.
5. Continue to subculture the cells in EX-CELL® CD CHO
EX-CELL® CHO Protein-Free Media every 3 - 4 days for 3 - 4 passages using a seeding density
The transfer of cells from serum-supplemented medium directly of 5 x 105 cells/mL in 25 cm2 or 75 cm2 flasks. SAFC
into protein-free medium is not recommended. It is best to Biosciences recommends centrifugation of the cell
slowly reduce the concentration of protein in the culture suspension prior to passaging.
environment and allow the cells to adjust. EX-CELL® CHO
Serum-Free medium is compatible and well-suited for this 6. After 3 - 4 passages, centrifuge and resuspend the cells in
interim period. Once successful growth of CHO cells has been EX-CELL® CD CHO medium supplemented with 8 mM
established, they can be transferred into EX-CELL® PF CHO L-glutamine at a density of 3-5 x 105 cells/mL in 125 mL
medium. Most CHO cell lines do not require a step-wise shaker flasks.
reduction from EX-CELL® CHO medium and can be transferred 7. Continue to subculture cells in EX-CELL® CD CHO every
directly into EX-CELL® PF CHO medium. A slight reduction in 3 - 4 days as described in Step 6.
cell growth may be observed during the first few subcultures 8. Allow the cells to adapt to EX-CELL ® CD CHO for an
in this medium, but cells will rapidly adjust. additional 4 - 6 passages. Cells are considered fully adapted
to EX-CELL® CD CHO when growth rates return to normal
Most cells can be transferred directly from EX-CELL® CHO into and viabilities are above 95%.
EX-CELL® PF CHO medium without adaptation. The following
procedure is suggested for those cells that may require weaning 9. Cells can now be scaled up as necessary using standard
(i.e., high protein, serum-free or serum-supplemented cultures): procedures and densities.

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Adaptation from Suspension Cultures Cryopreservation
1. Resuspend the cells in EX-CELL CD CHO medium
® Freezing:
supplemented with 8 mM L-glutamine at a density of CHO cells grown in EX-CELL ® CHO medium have been
5 x 105 cells/mL in 125 mL shaker flasks. successfully frozen in liquid nitrogen and recovered without
the reintroduction of serum. However, it is necessary to handle
2. Incubate the cells at 37 C in a humidified incubator with the cells gently and freeze the cells under carefully controlled
8 - 10% CO2. conditions.
3. Continue to subculture the cells in EX-CELL® CD CHO 1. Choose cultures in logarithmic growth with viabilities
every 3 - 4 days for 3 - 4 passages using a seeding density above 90%.
of 5 x 105 cells/mL in 125 mL flasks. SAFC Biosciences
recommends centrifugation of the cell suspension prior 2. Prepare a freezing medium consisting of 45% cold
to passaging. EX-CELL® CHO medium, 45% conditioned medium and
10% dimethyl sulfoxide (DMSO). Alternatively, cells can
4. After 3 - 4 passages, centrifuge and resuspend the cells in be frozen in 90% fresh medium and 10% DMSO.
EX-CELL® CD CHO medium supplemented with 8 mM
L-glutamine at a density of 3-5 x 105 cells/mL in 125 mL 3. Centrifuge the cells at 200 g for 5 minutes. Remove the
shaker flasks. supernatant.
5. Continue to subculture cells in EX-CELL® CD CHO every 4. Resuspend the cells in the freezing medium at 1 x 107
3 - 4 days as described in Step 4. cells/mL.
6. Allow the cells to adapt to EX-CELL® CD CHO for an 5. Rapidly transfer 1 - 2 mL of this suspension to sterile
additional 4 - 6 passages. Cells are considered fully adapted cryovials.
to EX-CELL® CD CHO when growth rates return to normal 6. Cells can now be scaled up as necessary using standard
and viabilities are above 95%. procedures and densities.
7. Cells can now be scaled up as necessary using standard 7. For long-term storage, transfer the vials to liquid nitrogen
procedures and densities. vapor.

Culture Techniques Thawing:


CHO cells are normally grown at 37 ± 1 C and 5 - 10% CO2. 1. Rapidly thaw a vial of frozen cells in a 37 C water bath.
Allow the medium to warm to room temperature prior to use
(protect from light). Once fully adapted, the cells should be 2. Transfer the cells aseptically to a centrifuge tube containing
subcultured at a seeding density of at least 2-4 x 105 cells/mL 5 - 10 mL of cold EX-CELL® CHO medium.
in shaker flasks. Recommended working volumes are 30 mL 3. Using low-speed centrifugation, pellet the cell suspension
cell cultures in 125 mL shaker flasks and 60 mL cultures in at 200 g for 5 minutes and carefully decant the supernatant
250 mL shaker flasks. Shaker speed should be approximately without disturbing the cell pellet.
125 rpm ± 5 rpm. Optimal seeding densities should be 4. Resuspend the cells in 5 mL of EX-CELL® CD CHO.
determined by the researcher for each application and cell
type. 5. Count the cells for viability and transfer to a sterile shaker
flask at a seeding density of 2.5-6 x 105 cells/mL.
When passing the cells, medium carry over should not exceed 6. When the culture has reached a density of 1-2 x10 6
25% of the final volume. If carry over exceeds 25%, cells/mL, passage the cells using standard cell culture
centrifugation is recommended. For best results, centrifugation techniques.
is recommended regardless of medium carryover. Cells
propagated in serum-free media are extremely fragile. For
successful results, care must be taken when subculturing cells.
Standard techniques of centrifugation must be modified to
include low-speed centrifugation to prevent damage to cells
that have been propagated in serum-free medium.

During adaptation, trypsin should be avoided if possible. If


trypsin is used, normal concentrations may be used, but
incubations should be carried out at 4 C, and exposure time
should be minimal. SAFC Biosciences recommends the use of
a soybean trypsin inhibitor (0.1%), or sedimentation by
centrifugation to remove the trypsin. Soybean trypsin inhibitor
should be used with caution, as it is toxic to some cell types.
Cells may also be dislodged with NO-ZYMETM (Catalog No.
59226C), a non-enzymatic dissociating agent.

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Rapid Selection of Optimal Formulations for Divergent Clones Through Screening
Chinese Hamster Ovary Media Library
Avril A. Lawshé, Min Zhang, Ashley Smith, Wade Nudson, James S. Ross and Matthew V. Caple
Cell Sciences & Development, SAFC Biosciences, Saint Louis, Missouri 63103
ESACT 2007 Poster#XXXX

Abstract Screening growth assays on CHO-S and the recombinant CHO-S cell lines using the CHO Media Library
The successful culture of Chinese Hamster Ovary (CHO) cells can be a challenging Cell Line 1 Cell Line 2
endeavor. Historical data shows that CHO clones have diverse nutritive requirements. a) b)
Selecting a medium designed specifically for a CHO cell line is a key component for CHO-S Recombinant CHO-S
achieving optimal growth and productivity. With the wide variety of media available, 8.0E+06 8.0E+06
narrowing the number of formulations for screening can be time consuming and
can impede the development process. After screening a battery of media, we have 7.0E+06 7.0E+06
narrowed the field to a finite collection of formulations under the classification of 6.0E+06 6.0E+06
the SAFC Biosciences’ CHO Media Library. Each of the formulations found within 5.0E+06 5.0E+06

Cells/mL

Cells/mL
the CHO Media Library have been chosen for their ability to meet the divergent
needs of numerous CHO cell lines, including DUX B11, CHO-K1 and CHO-S. The 4.0E+06 4.0E+06
majority of these formulations have a proven track record in industrial applications 3.0E+06 3.0E+06
and the data shows that, with or without adaptation, several formulations can be 2.0E+06 2.0E+06
quickly selected for further investigation after performing a growth assay using the
1.0E+06 1.0E+06
library. Performance of each formulation is evaluated based on: peak cell density,
integrated cell days, viability, culture longevity and, where applicable, volumetric 0.0E+00 0.0E+00
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18
productivity. Most offerings in the library are free of animal-derived components Culture Time (days) Culture Time (days)
while others are truly chemically defined (CD).
A B C D E F G A B C D E F G
H I J K L M N H I J K L M N

Introduction CHO-S Recombinant CHO-S


c) d)
SAFC Biosciences (SAFCB) has a diverse collection of cell culture formulations for 100 100
use with CHO cells. Previously, as cell lines arrived for media development, there 90 90
was no specific subset of media for screening. While leading to the creation of 80 80
Percent Viable

Percent Viable
many successful media formulations, this method had the possibility of omitting 70 70
formulations with better potential. After testing multiple cell lines in the vast array 60 60
of SAFCB media, the best possible subset has been labeled the CHO Media Library. 50 50
This current selection of media allows a more consistent starting point for new 40 40
media screening projects. 30 30
20 20
10 10
0 0
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Culture Time (days) Culture Time (days)
Materials and Methods
A B C D E F G A B C D E F G
Cell lines and media H I J K L M N H I J K L M N
The CHO cell lines tested with CHO Media Library were CHO-S (Invitrogen) and
a recombinant CHO-S line that produces human IgG. The CHO-S stock culture
was maintained in Gibco CD CHO Medium (Invitrogen) while the recombinant Figure 2 a & c: Cell Line 1 - Parental CHO-S
CHO-S line was cultivated in a proprietary animal-component free formulation. Figure 2 b & d: Cell Line 2 - Recombinant CHO-S
All SAFCB and competitor media were supplemented with 4 mM L-Glutamine. By evaluating the cell density and viability curves, the differences can be seen in the peak cell density and longevity of the cultures. For cell line 1, formulations F, B and A have the best growth,
Cultures were inoculated directly from stocks into TPP™ 50 mL bioreactor tubes respectively, based on peak viable cell density. For cell line 2, the best growth is seen in formulations A, C, M and L. These differences in media preference illustrate the distinctions between the parental
containing the applicable formulations for testing. Each condition was assayed CHO-S line and the recombinant CHO-S line.
in duplicate and averaged.
Productivity assay IgG-producing recombinant CHO-S cell line screened in CD formulations from the CHO Media Library and competitors
Human IgG: IgG concentrations were measured by Protein G affinity
chromatography. SAFCB Formulations Competitor Formulations

a)
Recombinant CHO-S b) Recombinant CHO-S
8.0E+06 8.0E+06

Results 7.0E+06 7.0E+06


6.0E+06 6.0E+06
CHO Media Library Screening 5.0E+06 5.0E+06
Cells/mL

Cells/mL

4.0E+06 4.0E+06
3.0E+06 3.0E+06
SAFCB Diverse 2.0E+06 2.0E+06
Media Offerings 1.0E+06 1.0E+06
0.0E+00 0.0E+00
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
The SAFCB medial collection is Culture Time (days) Culture Time (days)
screened with multiple cell lines Competitor 1 Competitor 2
A G H L M N Competitor 3 Competitor 4

CHO c) Recombinant CHO-S d) Recombinant CHO-S


Media
Library 700 700
A G H Competitor 1
The CHO Media Library is used as Competitor 2
600 L M N 600 Competitor 3
a starting collection for screening Competitor 4
customer cell lines and clones
500 500
IgG mg/L

IgG mg/L

400 400

300 300
3 - 5 Specific
Formulations 200 200

100 100
Figure 1: Multiple CHO cell lines were screened with the diverse CHO media formulations
available from SAFC Biosciences. The best of these formulations have been designated the CHO 0 0
Media Library. This collection constitutes a starting point for incoming screens of customer CHO 8 10 12 8 10 12
clones. The screen allows for the identification of the best 3 – 5 formulations for a specific clone Culture Time (days) Culture Time (days)
or group of clones.
Figure 3 a & c: CHO Media Library
Figure 3 b& d: Competitors
When comparing CD formulations from the CHO Media Library with those available from competitors, there is a range among the peak viable cell densities and volumetric productivity. Also evident is
the distinction between formulations that promote good growth versus ones that promote higher productivity.

Conclusions
• SAFC Biosciences has a wide range of media that has been narrowed to a collection called the CHO Media Library which serves as a starting point for screening customer CHO cell lines.
• Based on assay parameters (i.e. peak cell density, viability, longevity and productivity), the best performing formulations are defined for a specific cell line.
• A full screen of the CHO Media Library is suggested, even when the parental cell line is known, because a recombinant cell line may have a distinct preference for alternate formulations.
• When the offerings from the CHO Media Library are screened against competitor formulations, the diversity among the formulations is further amplified by the differences in growth promotion and volumetric productivity.

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Warranty, Limitation of Remedies
SAFC Biosciences warrants to the purchaser for a period of one year from date of delivery that this product conforms to
its specifications. Other terms and conditions of this warranty are contained in SAFC Biosciences’ written warranty, a
copy of which is available upon request. ALL OTHER WARRANTIES, EXPRESSED OR IMPLIED, INCLUDING THE IMPLIED
WARRANTY OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE, ARE EXCLUDED. In no case will SAFC
Biosciences be liable for any special, incidental, or consequential damages arising out of this product or the use of this
product by the customer or any third party based upon breach of warranty, breach of contract, negligence, strict tort,
or any other legal theory. SAFC Biosciences expressly disclaims any warranty against claims by any third party by way of
infringement or the like. THIS PRODUCT IS INTENDED FOR PURPOSES DESCRIBED ONLY AND IS NOT INTENDED FOR
ANY HUMAN OR THERAPEUTIC USE.
Additional Terms and Conditions are contained in the product Catalog, a copy of which is available upon request.

The SAFC BiosciencesTM logo, SAFC BiosciencesTM, SER-TAIN™ and NO-ZYMETM are
trademarks of Sigma-Aldrich Biotechnology L.P.

EX-CELL® is a registered trademark of SAFC Biotechnology L.P.

The GS SystemTM is a trademark of Lonza Biologics.

© 2008 SAFC Biosciences, Inc.

Issued April 2008 T096

United States Europe Asia Pacific


SAFC Biosciences, Inc. SAFC Biosciences Ltd. SAFC Biosciences Pty. Ltd.
13804 W. 107th Street Smeaton Road, West Portway 18-20 Export Drive
Lenexa, Kansas 66215 Andover, Hampshire SP10 3LF Brooklyn, Victoria 3025
www.safcbiosciences.com USA
Phone +1 913-469-5580
UNITED KINGDOM
Phone +44 (0)1264-333311
AUSTRALIA
Phone +61 (0)3-9362-4500
Toll free-USA 1 800-255-6032 Fax +44 (0)1264-332412 Toll free-AUS 1 800-200-404
Fax +1 913-469-5584 E-mail info-eu@sial.com Fax +61 (0)3-9315-1656
E-mail info-na@sial.com E-mail info-ap@sial.com