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Introduction: The human circulatory system is a collection of structures thorough which oxygen and nutrient rich blood flows to all tissues of the body for metabolism and growth, and to remove metabolic wastes. The blood is pumped to these tissues by the heart through a circuit composed of arteries, arterioles, capillaries, venules, and veins. Oxygenated blood is pumped to the tissues from the left side of the heart, whereas deoxygenated blood is pumped to the lungs from the right side of the heart. This circuit where gas exchange takes place within the alveoli of the lung is very important and is known as the pulmonary circuit. When the body is exercised changes can take place in the circulatory system that allow more blood to pass to actively respiring muscle cells and less to nonmuscular tissue. Increased heart rate, arterial pressure, body temperature, and breathing rate also occur during exercise. Arterial blood pressure is directly dependant on the amount of blood pumped by the heart per minute and the resistance to blood flow through the arterioles. This is an important measurable aspect of the circulatory system and it is measured using a sphygmomanometer. This device has an inflatable cuff that connects to a hand pump and a pressure gauge, graduated in millimeters of mercury, by rubber tubing. The cuff is wrapped around the upper arm and inflated, the person taking the pressure then listens for two sounds and observes the gauge to determine what the blood pressure is. The systolic number is determined by the first noise heard as the cuff is deflated, and the diastolic number is determined by the last distinct noise heard. Hypothesis: From this experiment it is expected that a subject’s heart rate and blood pressure will change during rest and exercise based on how physically fit they are. If the subject is in good shape the heart rate will not increase significantly and the blood pressure will increase. The opposite is true of someone in poor shape. Materials: The materials used in this experiment include a blood pressure kit, alcohol swabs, a stopwatch, two depression slides, a cotton ball, four rubber bands, a pipet, a petri dish, a Daphnia culture, a stereomicroscope, and some ice. Methods: A. Measuring Blood Pressure: To measure blood pressure, one member of the lab group sat down in a chair, rolled up his sleeve, and then the sphygmomanometer cuff was placed around his upper left arm at heart level. The cuff was then pumped to 200mm Hg, which is safely higher than the blood pressure of the subject. The stethoscope was then placed in the well of the subject’s elbow, where the brachial artery is located, and pressure was slowly released as the taker listened for a pulse. The pressure on the gauge was noted when first sound of Korotkoff was heard, which is the pressure that blood is first able to pass through the artery during systole, representing systolic pressure. The sounds of Korotkoff are heard between the systolic and diastolic blood pressures. The
Physical Fitness Test: The first numbers recorded from this section of the experiment were those of standing vs. stood up. resting blood pressure. The subject remained lying down for another two minutes. To do this a member of the lab group had to lie down on a table for five minutes. After five minutes the subject’s blood pressure was taken while he was still lying down and the numbers were recorded in Table 2. Average Blood Pressure Systolic Pressure Diastolic Pressure Age in Years Men Women Men Women 10 103 103 69 70 11 104 104 70 71 12 106 106 71 72 13 108 108 72 73 14 110 110 73 74 15 112 112 75 76 16 118 116 73 72 17 121 116 74 72 18 120 116 74 72 19 122 115 75 71 20-24 123 116 76 72 25-29 125 117 78 74 30-34 126 120 79 75 35-39 127 124 80 78 40-44 129 127 81 80 45-49 130 131 82 82 50-54 135 137 83 84 55-59 138 139 84 84 60-64 142 144 85 85 65-69 143 154 83 85 70-74 145 159 82 85 B.diastolic pressure is the reading of the gauge at the time the sounds of Korotkoff can no longer be heard. and their blood pressure was taken again. The standing systolic pressure was subtracted from the resting systolic pressure and recorded in Table 2. The subject’s blood pressure was taken two more times and an average was calculated and recorded in Table 1. A chart was used to determine the number of points received by the subject and recorded in Table 3. .
This was continued for 15 seconds. The endurance test was the last leg of this section of the experiment. Into the dish was then added ice water and the same Daphnia’s heart rate was determined and recorded in Table 5. A chart was then used to determine the number of points the subject received for this section and recorded in Table 3. multiplied by four. First the pulse was taken right after the exercise for 15 seconds and multiplied by four. The data was recorded in Table 4. The subject had to lie down for five minutes. From this number the resting heart rate was subtracted and recorded in Table 3. . After five minutes the subject’s pulse was taken and recorded in Table 3. Next the Baroreceptor reflex test was given to the subject. and the results placed in Table 5. These heart rates were then compared to the standing heart rate. The heart of the largest Daphnia was then located under the stereomicroscope and the number of beats in 15 seconds was determined. 1cm deep and the slides were placed into it. and then his pulse was taken at several intervals. Next the resting heart rate was determined by having the subject lie down on a table for five minutes. Gradually warm water was added and the heart rate was taken at five minute intervals until the normal heart rate is noted. stand up quickly. Taken by the subject was the radial artery pulse by counting the number of beats for 30 seconds. These results were put in Table 5. Several Daphnia were placed on the slide with a pipet and the other slide was placed on top of this slide and wrapped together with a rubber band on each end. and record the pulse. Another chart was used to determine the amount of points the subject received for this section and that number was also recorded in Table 3.The next part of this section is where the subject’s standing heart rate was determined. Then the pulse was taken every 30 seconds for 120 seconds after that. Next. That number was multiplied by 2 to obtain the number of beats per minute. The amount of time it took for the subject’s heart rate to return to normal was figured and a chart was used to award points. C. the standing heart rate was subtracted from the rate taken right after exercise. That number was recorded in Table 3. To do this the subject stepped up with one foot onto an 18 inch high surface and then brought up the other foot onto the surface. Investigating Heart Rate in Daphnia: Two depression slides were obtained and a small piece of cotton was placed in the center of one of the slides. This was repeated one more time after that as well. Once again a chart was used to determine the number of points the subject received for this section of the experiment and the number was recorded in Table 3. A petri dish was filled with roomtemperature water. and yet another chart was used to award points.
Lying to Standing Change Systolic 110 120 10 Diastolic 72 72 0 Table 3 Fitness Points Activity Change in Blood Pressure Result 10 Fitness Points 3 . Resting Blood Pressure Position Lying Down 5 min.Results: Table 1 Blood Pressure Trial 1 Trial 2 Trial 3 Average Systolic 115 115 115 115 Table 2 Diastolic 72 70 74 72 Standing vs.
of Beats 24 19 35 35 35 X4= X4= X2= X2= X2= Heart Rate 96 76 70 70 70 Total Score 17 to 18 14 to 16 8 to 13 7 or less Cardiovascular Fitness Excellent Good Fair Poor .Standing Pulse Rate Resting Pulse Rate Baroreceptor Reflex Heart Rate Recovery After Exercise Heart Rate Increase After Exercise Total Points 78 64 76 28 18 - 3 3 3 4 2 18 Table 4 Heart Rate After Exercise Interval 0 to 15 sec. 91 to 120 sec. 31 to 60 sec. No. 61 to 90 sec. 16 to 30 sec.
What changes occur in the circulatory system when a person stands up from a prone position? How do these changes affect the heart rate and blood pressure of the individual? The circulatory system is not working very hard when a person is at rest so when that person stands up suddenly the blood pressure and heart rate of that person increase. and the heart in particular. 3. Why is high blood pressure dangerous? What health problems does it lead to? High blood pressure is dangerous because the heart has to work to hard to push the blood through the various veins and arteries and a heart attack can occur. of a conditioned athlete differ from that of a person in poor shape? The heart of a conditioned athlete is stronger because it has been worked harder pumping more blood when that person exercises. What sort of behaviors encourage high blood pressure? Why? Eating fatty foods and not exercising cause high blood pressure because the heart is not working hard to pump the blood.Questions: 1. How does the circulatory system. 4. Table 5 Temperature (C) Room Temperature 0 to 5 10 15 Heartbeats per Minute 200 84 160 152 . The heart of a person in poor shape has not been worked as hard. 2. which makes it weak.
Endotherms have a constant body temperature and do not change their metabolic rate strictly based on environmental conditions. If this experiment were performed on a human subject. but not to the extent that the Daphnia heart did.20 25 30 35 204 200 212 216 Change in Metabolic Rate Temperature Range 0-10 10-20 20-30 Rate of the reaction (change in heart rate) Q10 =1. so their metabolic rates also change with this. 2. Why does the rate of activity of ectothermic organisms increase with a rise in the temperature of its environment? How is this different from an endothermic organism? Ectothermic organisms’ body heat is determined by the environment.04 Questions: 1.9 Q10 =1.275 Q10 =1. . what results would you expect? Explain. A human’s heart would also be affected by the temperature changes.
. Blood pressure is so important to a person’s health it is checked at every visit to the doctor or hospital. Conclusions: Cardiovascular fitness is very important to living a healthy life.Error Analysis: The only possible source of error in this lab would have been the slight misreading of the gauge on the sphygmomanometer. If one doesn’t exercise and eat healthy foods they run a risk of being in poor shape and having a heart attack or other serious things. Heart rate and blood pressure readings can give a person a good idea about how healthy they are or how healthy they need to be.
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