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Test K-DES® N.

2 Applied in Birds avoids the decomposition
and allows the preservation of organic matter.

www.abc-des.com

TO SUMMARIZE:

- Product K-DES® is a 25% water solution that avoids the decomposition and
allows the preservation of organic matter, not only when applied by spraying but
also when applied by immersion.

- Product K-DES® is a 25% water solution that avoids the decomposition and
allows the preservation of organic matter, even after the process of
decomposition has already started.

- Product K-DES® can be reused without reducing its preserving effect, avoids
the decomposition and allows the preservation of organic matter.

- When used by spraying, product K-DES® preserves and avoids the
decomposition and allows the preservation of organic matter for over 96 hours.
The application of a 25% water solution of K-DES® provides the samples with
an insect repellent and leaves them soft enough to ease its future treatment and
elimination.

- For the reason that K-DES® must be applied by spraying when a farming scope
is concerned, and waiting for new tests for this to be confirmed, we can draw
the conclusion that bodies can remain for a period of 96 hours without great in-
convenience for the environment.

- K-DES® is a nontoxic product, nor ADR, which avoids the decomposition and
allows the preservation of organic matter, helping the bio safety of the
environment in which it is applied.

http://www.abc-des.com/informacioning.htm
®
TEST K-DES II

Completed between 26th of May
and 7th of June 2006

www.abc-des.com
INDEX

Previous notes on the test .................... ¡Error! Marcador no definido.

Day 0. Start – 26-05-2006 ................................................................... 3

Day 1 – 27-05-2006............................................................................. 5

Day 2 – 28-05-2006............................................................................. 6

Day 3 – 29-05-2006............................................................................. 9

Day 4 – 30-05-2006........................................................................... 18

Day 5 – 31-05-2006........................................................................... 22

Day 6 – 1-06-2006............................................................................. 26

Day 7 – 02-06-2006........................................................................... 30

Day 8 – 03-06-2006........................................................................... 33

Day 9 – 04-06-2006........................................................................... 36

Day 10 – 05-06-2006......................................................................... 38

Day 11 – 06-06-2006......................................................................... 40

Day 12 – 07-06-2006......................................................................... 42

Statistics summary ............................... ¡Error! Marcador no definido.

Conclusions.......................................... ¡Error! Marcador no definido.
0BPrevious notes on the test Test K-DES® II

Previous notes on the test

Objective

The aim is to carry out a study of the application of K-DES® on birds, in order to as-
sess its effect as a disinfectant and preservative of organic matter.

Test conditions

Across this document, daily observed conditions are referred to. It can be added up
that samples have been maintained in the open air, therefore under the effects of
the environment, and subjected to likely flies and insects action, proper of the urban
environment in which they were staying.

The test took place over a period between 26th of May and 7th of June 2006, in the
city of Vigo.

It has to be remarked that over this period, we have been under very high tempera-
tures, not proper of the region, nor the time of the year, many times over 35ºC, fact
that is important to be noticed, due to the way high temperatures affect to the proc-
esses to be observed.

For the assessment of the treatment, variables such as appearance, smell, flies and
insects presence, were used. All of them are symthoms of decomposition that are
not subjective issues anymore, due to the important effects observed as a conse-
quence of K-DES® application.

The person in charge of the study, Mr. Javier Chávez, is an expert in embalming,
with many years of experience in human and animal corpse handling, which gives
him a knowledge to detect small details, proper of organic matter decomposition,
which are referred to in this report.

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1BDay 0. Inicio – 26-05-2006 Test K-DES® II

Day 0. Inicio – 26-05-2006

Before the start of the test

Now we can see three birds right before the application of K-DES®. The birds were
slaughtered after 20 hours, with the application being done at 9,30 pm, having an air
temperature of 25ºC, time at which the next pictures were taken. The study consists
of three hens, one of them used as a reference sample, which has been given label
A. Samples labelled as B and C were sprayed on with a 25% water solution of the
product.

P5260271

REFERENCE - A

P5260272

SPRAYING – B

P5260273

SPRAYING – C

-3-
1BDay 0. Inicio – 26-05-2006 Test K-DES® II

Start of the test

OBSERVATIONS

K-DES® is a translucent product, not crystalline, of quick action and with a very par-
ticular smell, what allows to differenciate treated from not treated samples, in a se-
cure and easy way.

Application of the product is done in an easy way by pressurized spraying on the bodies
to be treated, which easies the adhearence of the product to the body surface, thus
getting a high grade of impregnation.

As mentioned above, 3 birds were selected, and classified as follows:

Sample A was used as a reference sample, not being previously treated.

Samples B and C were sprayed on with a 25% water solution of K-DES®.

P5260275

SPRAYING – B

This is the appearance of the sample
after a superficial application was done
by spraying with a 25% water solution
of K-DES®.

P5260276

SPRAYING – C

This is the appearance of the sample
after a superficial application was done
by spraying with a 25% water solution
of K-DES®.

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2BDay 1 – 27-05-2006 Test K-DES® II

Day 1 – 27-05-2006

The test was started on 27th may, at 8 pm, having an air temperature of 29ºC,
though temperatures around 34ºC were reached across the day.

During the first 24 hours, not many changes can be observed just by looking at the
sample. However, K-DES® smell can perfectly be perceived in samples B and C. An
unpleasant smell can be noticed from hen A.

P5270279

REFERENCE - A

P5270280

SPRAYING – B

P5270281

SPRAYING – C

-5-
3BDay 2 – 28-05-2006 Test K-DES® II

Day 2 – 28-05-2006

Day second of tudy. Day 28, at 9 pm, having an air temperature of 30ºC. Tempera-
tures of 34ºC were reached across the day.

Sample A - Reference

On this day, important changes can be observed just by looking at it, mainly when
smell is concerned. An unpleasant smell is perfectly perceptible in case A, with flies
being present. A slight swelling can be observed in the head.

It has to be taken into account that, obviously, all along the day, the already men-
tioned flies have been in contact with the body, with the process of egg-laying being
started. This simple fact can represent a big problem in the farming environment,
where its proliferation will be much greater and faster than in an urban environment
similar to the one in which test took place.

P5280283

REFERENCE - A

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3BDay 2 – 28-05-2006 Test K-DES® II

P5280284

REFERENCE - A

It shows the presence of flies.

We can see the flies and the head swelling in sample A.

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3BDay 2 – 28-05-2006 Test K-DES® II

Samples B and C- Spraying

As for the cases B and C, K-DES® smell is still perceptible and, just by looking at
them, no great changes can be observed.

As it can be observed from the next pictures, the colour of the plumage of samples
that have been treated with K-DES® is slightly clearer than sample A’s and at this
moment, flies are not around, despite they are two metres away from sample A,
aproximately.

The fact that insects are not closer is because of K-DES® repellent effect, what
shows that, if K-DES® is applied, the presence of outer agents contributing to the
putrefaction process is avoided, for instance flies.

P5280286

SPRAYING - B

P5280287

SPRAYING - C

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4BDay 3 – 29-05-2006 Test K-DES® II

Day 3 – 29-05-2006

After 17 hours

Third day of study. On day 29, these first pictures have been taken after 17 hours,
on an air temperature of 34ºC. Even higher temperatures have been reached across
the day.

Sample A - Control

Due to the fact that a remarkable deterioration was observed in sample A on the day
before, we decided to carry out a tighter vigilance on it, in order to intervene if nec-
essary. So data takes were done earlier.

Sample A smell, 69 hours after the slaughter, is nauseating and the swelling is no-
table. Besides, we observe another sign of decomposition, say dehydration. There-
fore we can draw the conclusion that the body is in an advanced stage of post-
mortem processes. Several signs, as eye movement, allow us to claim the presence
of larvae inside the animal.

P5290024

REFERENCE - A

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4BDay 3 – 29-05-2006 Test K-DES® II

P5290025

REFERENCE - A

We can see the swelling in sample A, and the process of dehydration, since the
damp which is seen on the carton comes from the inside of the body.

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4BDay 3 – 29-05-2006 Test K-DES® II

Samples B and C- Spraying

In cases B and C, the smell of decomposition starts to be perceptible, though slight.
However, swelling is minimal, as it can be seen in case B, where it can be observed
around the eye. In case C, a slight swelling can be observed in the body. The pic-
tures below show these differences:

P5290028

SPRAYING - B

P5290029

SPRAYING - B

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4BDay 3 – 29-05-2006 Test K-DES® II

P5290030

SPRAYING - C

P5290031

SPRAYING - C

It can be observed that, different from sample A, swelling is smaller
and outside appearance is in a better state of preservation.

- 12 -
4BDay 3 – 29-05-2006 Test K-DES® II

Sample B – New spraying

At this moment, after three days from the start, and right after the check carried out
on sample A, we decided to do a new application of K-DES® on sample B, so as to
observe its evolution on the next days. Also, we decided to put off the second appli-
cation of K-DES® on sample C until the moment of the second check of the day (at
9,40 pm), thus checking the differences between the two ways of application.

P5290033

2nd SPRAYING – B

P5290034

2nd SPRAYING - B

- 13 -
4BDay 3 – 29-05-2006 Test K-DES® II

At 9,30 pm

Third day of study. Day 29, 9,40 pm, air temperature 25ºC.

Sample A – Reference

The pictures show that the swelling process in case A is still on, the smell is very
strong, more body fluids can be observed (remember that no application was done
on this sample, therefore, fluids observed are no other than the ones corresponding
with corpse dehydration). In addition, eye movement can be observed, what indi-
cates that it won’t take long for larvae to come outside.

P5290288

REFERENCE - A

P5290289

REFERENCE - A

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4BDay 3 – 29-05-2006 Test K-DES® II

P5290290

REFERENCE - A

At the moment of doing the second data take of the day, having into account the
deterioration of sample A and knowing that, in case of letting the natural process of
decomposition go on, the sample should be inmediately removed to avoid the risk of
infection, an application of K-DES® was done in order to observe the effect of the
product on a body in an advanced stage of decomposition.

First result, right after the application of the product, was unpleasant smell reduction.

P5290295

REFERENCE - A

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4BDay 3 – 29-05-2006 Test K-DES® II

Sample B - Spraying

Case B, on which a second application was done, after 17 hours (69 hours after the
slaughter), remains in a right state, no flies or other insects present, nor any signs
showing negative changes or deterioration.

P5290291

SPRAYING – B

P5290292

SPRAYING – B

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4BDay 3 – 29-05-2006 Test K-DES® II

Sample C - Spraying

In case C smell is still present, though not strong, and no changes of deterioration of
the sample can be observed. There are no flies, nor larvae and the swelling at this
moment is small.

A second application has also been done in this case, in order to check how long
corpses can be kept in a good state with a daily application after three days.

P5290293

2nd SPRAYING – C

P5290297

2nd SPRAYING – C

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5BDay 4 – 30-05-2006 Test K-DES® II

Day 4 – 30-05-2006

Fourth day of study. Day 30, data take carried out at 9,30 pm, having an air tem-
perature of 20ºC.

Sample A – Reference

In case A, larvae, known to be in the inside due to signs above mentioned, are al-
ready present in the outside. Before that, and in order to check the biocidal proper-
ties of the product, a new application of K-DES® was done, thus observing how they
move quickly to escape the action of the product.

P5300298

REFERENCE - A

P5300305

REFERENCE – A

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5BDay 4 – 30-05-2006 Test K-DES® II

P5300299

REFERENCE – A

Deterioration shown by this picture of sample A is noticeable and greater than the
other bird’s.

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5BDay 4 – 30-05-2006 Test K-DES® II

Samples B and C - Spraying

The state of preservation in cases B and C is good. We can see in the picture that
the swelling remains small, as well as the smell is slight, though reduced by the
product’s.

As it can be observed, after this period of time, corpse phenomena start to show. It
has to be remarked that, though flies are around, they don´t land on the body.

Another application of K-DES® is done for maintenance.

P5300300

SPRAYING – B

P5300301

SPRAYING – B

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5BDay 4 – 30-05-2006 Test K-DES® II

P5300306

SPRAYING – B

P5300302

SPRAYING – C

P5300303

SPRAYING – C

P5300309

SPRAYING – C

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6BDay 5 – 31-05-2006 Test K-DES® II

Day 5 – 31-05-2006

Fifth day of study. Day 31, it’s 9,30 pm and air temperature is 24ºC.

Sample A – Reference

In case A, larvae, as a consequence of K-DES® application, are dead and smell,
though still present, is tolerable.

P5310310

REFERENCE - A

P5310311

REFERENCE - A

P5310313

REFERENCE - A

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6BDay 5 – 31-05-2006 Test K-DES® II

P5310312

REFERENCE - A

It can be observed that larvae coming from the inside of the body has been elimi-
nated as soon as they got in touch with the product remaining on the surface on
which the sample was staying.

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6BDay 5 – 31-05-2006 Test K-DES® II

Samples B and C - Spraying

In cases B and C, we can observe a swelling that is proper of decomposition proc-
esses, but smell is not strong and no larvae, nor any other symthom of degradation
can be seen. Only a minimal amount of flies is present, and as it ocurred in earlier
days, they don´t land on the bodies.

Another application of K-DES® was done in order to check how long a bird can be
kept in a place without being a health problem for its environment.

P5310314

SPRAYING – B

P5310315

SPRAYING – B

- 24 -
6BDay 5 – 31-05-2006 Test K-DES® II

P5310316

SPRAYING – C

P5310317

SPRAYING – C

- 25 -
7BDay 6 – 1-06-2006 Test K-DES® II

Day 6 – 1-06-2006

Sixth day of study. Day 1, at 9 pm, air temperature is 28ºC, though temperature has
reached around 34ºC along the day.

Sample A – Reference

In case A, not many changes can be perceived, only smell is perceptible, but toler-
able. However, when applying the maintenance product, many larvae started to
come out from the inside of the corpse, what indicates that larvae reproduction oc-
curs very fast.

After product application, larvae came outside due to the product repellent effect,
and died after a few seconds. As well as larvae, a small collection of other necro-
phagous insects appeared and died almost at once. Besides, it can be observed
that, due to K-DES® maintenance dosage, there are no flies around, despite the bird
is in an advanced stage of decomposition.

Before product application.

P6010318

REFERENCE - A

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7BDay 6 – 1-06-2006 Test K-DES® II

P6010319

REFERENCE - A

After application.

P6010327

REFERENCE - A

P6010325

REFERENCE - A

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7BDay 6 – 1-06-2006 Test K-DES® II

Samples B and C - Spraying

In cases B and C there are no signs of corpse fauna. Only internal changes from
decomposition can be observed, i.e. body swelling.

It is due to bacteria proliferation produced by the autolysis phenomenon. Along the
process, these bacteria produce gases that stay in the inside of the body, thus caus-
ing body swelling.

P6010320

SPRAYING – B

P6010321

SPRAYING – B

- 28 -
7BDay 6 – 1-06-2006 Test K-DES® II

P6010322

REFERENCE - C

P6010323

REFERENCE - C

- 29 -
8BDay 7 – 02-06-2006 Test K-DES® II

Day 7 – 02-06-2006

Seventh day of study. Day 2, air temperature is 25ºC, at 8,30 pm, moment showed
by the pictures.

Sample A – Reference

As it can be seen in next pictures, internal larvae that have been in touch with the
product, though many of them, are dead, as well as necrophagous insects. Flies do
not come closer to the corpse and smell, though strong, is tolerable.

Having into account the state of deterioration of the sample and once the effect of
the product K-DES® over a body in an advanced stage of decomposition has been
shown, it was decided not to do any other application and to remove the sample
from the study.

P6020329

REFERENCE - A

P6020330

REFERENCE - A

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8BDay 7 – 02-06-2006 Test K-DES® II

Samples B and C- Spraying

Cases B and C remain in a good state with no signs of corpse fauna. A slight un-
pleasant smell is perceived, though reduced by the application of K-DES®.

P6020331

SPRAYING – B

P6020332

SPRAYING – B

P6020334

SPRAYING – C

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8BDay 7 – 02-06-2006 Test K-DES® II

P6020333

SPRAYING – C

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9BDay 8 – 03-06-2006 Test K-DES® II

Day 8 – 03-06-2006

Eighth day of study. Day 3, pictures were taken at 10,15 pm, having an air tempera-
ture of 22ºC. As well as in earlier days, temperature has been high along the day.

Sample A – Reference

Sample A has been removed.

Samples B and C – Spraying

Smell in cases B and C is still slight, corpse fauna is now present in a small amount
and only in the cloaca zone, in sample B. Unlike larvae observed in sample A, these
are small, in a small quantity, and no necrophagous insects can be seen.

Anyway, after all these days, it could be said that samples remain in a good state
and not causing any problem to its environment, since the presence of insects and
flies is very scarce and, as well as in earlier days, they don’t land on the samples.

P6030335

SPRAYING – B

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9BDay 8 – 03-06-2006 Test K-DES® II

P6030338

SPRAYING – B

Picture of the cloaca.

It can be observed that
the size of dead larvae is
very small (unlike ones
in picture of page 23)
after getting in touch with
the product, no presence
of other necrophagous
insects being perceived,
despite the few days that
have already passed.

Sample A – Reference
(page 23).

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9BDay 8 – 03-06-2006 Test K-DES® II

P6030340

SPRAYING – C

P6030339

SPRAYING – C

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10BDay 9 – 04-06-2006 Test K-DES® II

Day 9 – 04-06-2006

Ninth day of study. Day 4, pictures taken at 10 pm, having an air temperature of
22ºC. Very high temperature along the day, close to 30ºC.

Sample B and C – Spraying

With the aim of checking how long the samples can be kept, it was decided not to do
any other application of K-DES®, so as to check the efficiency of the remaining
product stuck to the body.

After 9 days from the start of the test, smell of putrefaction is still slight and larvae
amount is still minimal in the cloaca zone. The amount of present flies is scarce.
They fly around the samples rather than land on them.

It must be remarked that, though in case B there are small larvae in the cloaca zone,
no corpse fauna can be observed in sample C, bearing in mind that the second ap-
plication of K-DES® in sample C was made four hours afterwards than in sample B.
This may be caused by the difference existing between beings and individuals when
K-DES® application is concerned.

Humidity that can be observed in the attached pictures has not been caused by any
application of K-DES®, but by corpse dehydration itself, as it can be clearly seen.

P6040341

SPRAYING – B

- 36 -
10BDay 9 – 04-06-2006 Test K-DES® II

P6040342

SPRAYING – B

P6040343

SPRAYING – C

P6040344

SPRAYING – C

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11BDay 10 – 05-06-2006 Test K-DES® II

Day 10 – 05-06-2006

Tenth day of study. Day 5, pictures were taken at 9 pm, having an air temperature of
23ºC and day temperature reached over 30ºC.

Samples B and C – Spraying

Despite the time that has already past, samples are still in a good state, after 48
hours from the last application of the product. Unpleasant smell is still slight in both
samples, larvae amount is still minimal in the cloaca zone in case B, not being pre-
sent in case C. Flies amount, as in earlier days, is scarce, they don´t land on the
surface but are around the bodies.

P6050345

SPRAYING – B

P6050346

SPRAYING – B

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11BDay 10 – 05-06-2006 Test K-DES® II

P6050347

SPRAYING – C

P6050348

SPRAYING – C

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12BDay 11 – 06-06-2006 Test K-DES® II

Day 11 – 06-06-2006

Eleventh day of study. Day 6, pictures were taken at 9,20 pm. Air temperature is
28ºC and day temperatures have been close to 35ºC.

Samples B and C – Spraying

Next pictures were taken after 72 hours from the application of K-DES®. No out-
standing changes can be observed, with measurements being the same as in the
last days, i.e a slight smell perfectly tolerable, though in this case, no larvae nor flies
or insects can be observed around any of the samples.

P6060349

SPRAYING – B

P6060350

SPRAYING – B

- 40 -
12BDay 11 – 06-06-2006 Test K-DES® II

P6060351

SPRAYING – C

P6060352

SPRAYING – C

- 41 -
13BDay 12 – 07-06-2006 Test K-DES® II

Day 12 – 07-06-2006

Twelveth day of study. Day 7, measurement take was done at 6,20 pm on an air
temperature of 28ºC and, as well as in last days, temperatures along the day
reached over 35ºC.

Sample B and C – Spraying

After 12 days of test, and with no other application of the product, it can be observed
that the samples are in a good state of preservation, with a slight smell, perfectly
tolerable, and in a high stage of dehydration. However, larvae are not to be seen in
the outside, and the amount of flies near the corpses is minimal.

P6070353

SPRAYING – B

P6070354

SPRAYING – B

- 42 -
13BDay 12 – 07-06-2006 Test K-DES® II

P6070355

SPRAYING – C

P6070356

SPRAYING – C

- 43 -
14BStatistical Summary Test K-DES® II

Statistical Summary

Now, a chart summary of observations done by the expert, is shown.

Temperatures

Day 1 2 3 4 5 6 7 8 9 10 11 12
T at data take
instant(ºC)
29 30 25 20 24 28 25 22 22 23 28 28
Maximum T
(ºC)
34 34 34 32 33 34 34 32 30 30 35 35

Gráfica de Temperatura

40
35
Grados Centígrados

30
25
Hora toma datos
20
Máxima
15
10
5
0
1 2 3 4 5 6 7 8 9 10 11 12
Días

Note: It has to be remarked that temperature in Galicia on these dates were affected
by the irruption of a strong Saharian air current, having temperatures that are not
common for the days on which the test was carried out nor for the region.

On the other hand, it has to be noticed that the birds were left in the open air, on the
upper part of a building, on terraces exposed to an intense insolation, what caused
that temperatures might have been higher than the ones referred to in this docu-
ment, which are the ones officially registered.

- 44 -
14BStatistical Summary Test K-DES® II

Smell

Evaluation of registered data:

0 Proper smell of product K-DES®.

5 Unpleasant smell due to decomposition processes.

10 Unbearable smell due to decomposition processes.

Day 1 2 3 4 5 6 7 8 9 10 11 12
Sample A 5 8 10 3 5 5 5
Sample B 0 0 1 1 1 1 1 1 2 3 4 5
Sample C 0 0 1 1 1 1 1 1 2 3 4 5

Gráfica de Olor

12

10

8
Intensidad

Muestra A
6 Muestra B
Muestra C
4

2

0
1 2 3 4 5 6 7 8 9 10 11 12
Días

Note: It must be recalled that, an application of K-DES® was done on sample A on
the fourth day, due to its bad state, in order to check its effect once the sample was
in an advanced stage of decomposition.

- 45 -
14BStatistical Summary Test K-DES® II

Presence of external insects (flies and others)

Evaluation of registered data:

0 no insects.

5 considerable amount of insects.

10 many insects.

Day 1 2 3 4 5 6 7 8 9 10 11 12
Sample A 3 8 10 1 1 1 1
Sample B 0 0 0 1 1 1 1 1 1 1 2 3
Sample C 0 0 0 1 1 1 1 1 1 1 2 3

Gráfica de Presencia de Insectos Externos

12

10

8
Intensidad

Muestra A
6 Muestra B
Muestra C
4

2

0
1 2 3 4 5 6 7 8 9 10 11 12
Días

Note: It must be recalled that, an application of K-DES® was done on sample A on
the fourth day, due to its bad state, in order to check its effect once the sample was
in an advanced stage of decomposition.

- 46 -
14BStatistical Summary Test K-DES® II

Presence of internal insects (larvae and necrophagous)

Evaluation of registered data:

0 no larvae and/or necrophagous insects.

5 considerable amount of larvae and/or necrophagous insects.

10 many larvae and/or necrophagous insects.

Day 1 2 3 4 5 6 7 8 9 10 11 12
Sample A 0 3 5 6 7 8 9
Sample B 0 0 0 0 0 0 0 1 1 1 0 0
Sample C 0 0 0 0 0 0 0 0 0 0 0 0

Gráfica de Insectos Internos

10
9
8
7
Intensidad

6 Muestra A
5 Muestra B
4 Muestra C
3
2
1
0
1 2 3 4 5 6 7 8 9 10 11 12
Días

Note: it must be recalled that, larvae and necrophagous insects that were observed,
died once in touch with K-DES®.

- 47 -
14BStatistical Summary Test K-DES® II

Other signs of decomposition

Swelling

Evaluation of registered data:

0 no body swelling due to decomposition processes.

5 moderate body swelling due to decomposition processes.

10 big body swelling due to decomposition processes.

Day 1 2 3 4 5 6 7 8 9 10 11 12
Sample A 3 5 9 9 9 9 9
Sample B 0 0 1 1 2 2 2 2 2 2 2 2
Sample C 0 0 1 2 3 3 3 4 4 4 4 4

Gráfica de Hinchazón

10
9
8
7
Intensidad

6 Muestra A
5 Muestra B
4 Muestra C
3
2
1
0
1 2 3 4 5 6 7 8 9 10 11 12
Días

- 48 -
14BStatistical Summary Test K-DES® II

Dehydration

Evaluation of registered data:

0 no body dehydration due to decomoposition processes.

5 moderate grade of body dehydration due to decomposition processes.

10 high grade of body dehydration due to decomposition processes.

Day 1 2 3 4 5 6 7 8 9 10 11 12
Sample A 0 1 5 6 7 8 9
Sample B 0 0 0 0 0 0 0 0 1 2 2 3
Sample C 0 0 0 0 0 0 0 0 1 2 2 3

Gráfica de Deshidratación

10
9
8
7
Intensidad

6 Muestra A
5 Muestra B
4 Muestra C
3
2
1
0
1 2 3 4 5 6 7 8 9 10 11 12
Días

Note: It must be recalled that no new applications of K-DES® were done on samples
B and C after ninth day inclusive.

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15BConclusions Test K-DES® II

Conclusions

- The effect of the application of K-DES® by spraying a 25% water solution, re-
duces unpleasant smell, proper of organic matter putrefaction

- The effect of the application of K-DES® by spraying a 25% water solution, re-
duces the amount and size of larvae and necrophagous insects, proper of in-
ternal decomposition of animal corpses.

- The effect of the application of K-DES® by spraying a 25% water solution, re-
duces body swelling, proper of body putrefaction.

- The effect of the application of K-DES® by spraying a 25% water solution, re-
duces body dehydration, proper of animal corpses putrefaction.

- As a summary of all the above mentioned, we must draw the conclusion that
product K-DES® in a 25% water solution, causes the effect of preventing
decomposition of organic matter once it has been applied by spraying, thus
slowing down the process.

- Product K-DES® in a 25% water solution causes the effect of stopping the
process of decomposition of organic matter, even when it is in an advanced
stage.

- The application of a 25% water solution of K-DES® causes a repellent effect
against flies and insects, providing the bodies with protection against exter-
nal agents action, what can intervene in the process of putrefaction of or-
ganic matter.

- Product K-DES®, in a 25% water solution, if applied by spraying, preserves
and prevents organic matter from decomposition for a period of over 96
hours.

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15BConclusions Test K-DES® II

- Since the application of K-DES® in the farming scope will be done by spray-
ing, and waiting for new tests to confirm that, we can draw the conclusion
that bodies can be kept in a good state for a period of over 96 hours, without
being a great inconvenience for the environment.

- The repellent effect of K-DES® avoids that flies land on the corpses and the
process of egg-laying, as well as it prevents them from being a vehicle of in-
fection.

- Apart from the repellent effect of K-DES®, it also acts as a biocide against
larvae resulting from putrefaction, and eliminates them as soon as they come
outside.

- On the other hand we can draw the conclusion that if applied in the way it
has been done through this test, it means an alteration to corpse fauna de-
velopment. Although this development at an internal level cannot be avoided,
it has been modified and reduced, (having a smaller amount of larvae with a
smaller size). This phenomenon might be caused due to the impossibility of
larvae coming outside, because of the repellent and biocidal effect of the
product, or due to the alterations caused by the product at an external level,
which are transmitted to the interior of the corpse, thus changing internal
conditions.

- These results have been achieved from a reduced number of birds, but if ex-
trapolated to an exploitation with a bigger number of corpses, we reckon that
flies and insects would act much sooner and with a greater intensity, thus
accelerating the process of putrefaction, mainly in individuals that have not
been treated with K-DES® and so making the differences clearer to observa-
tion.

- So, we could draw the conclusion that, still more tests to be done that confirm
these results, the applicaiton of a 25% water solution of K-DES® by spraying
on an animal corpse after its death, and repeating the application every 48
hours, would slow down the processes of putrefaction and decomposition for
a period of over 96 hours, without causing unpleasant or harmful effects in
the environment where the corpse is being preserved, thus avoiding the

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15BConclusions Test K-DES® II

spreading of contagious illnesses and keeping a cleaner and more hygienic
environment.

- 52 -