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Step Ahead in Genomics Take the PCR and Transfection Challenge

Our KOD polymerases and transfection reagents are 100 % guaranteed.
KOD Polymerases: Meet the demands of next generation genomic amplification as ultra high fidelity tools for Synthetic Biology, Biofuels, Next Generation Sequencing (NGS), Plant & Animal PCR: • Ultra-high fidelity with speed and yield • Amplification of GC-rich (90 %) DNA • Long-range PCR (40 kb plasmid/phage & 24 kb genomic) • Proven compatibility with deriviatized dNTPs •  Minimal sample processing with ready-to-use protocols for accurate PCR in complex crude lysates • Generate less bias in NGS libraries

You have nothing to lose and everything to gain with our Performance Guarantee.
If you are not completely satisfied, for any reason, with the performance of EMD Millipore KOD Polymerases or transfection reagents, contact a Technical Support Specialist for assistance or full credit against future purchases. (see reverse for contacts) Request your free copy of our PCR Protocols or Transfection Guides.

www.emdmillipore.com/mobiochallenge

A. More DNA in less time
4.00 3.50 3.00 2.50 2.00 1.50 1.00 0.50 0.00

B. Great for GC-rich DNA
1 2 3 4 M1

C. Improved Next Gen. Seq. Bias
80 70 60 Coverage 50 40 30 20 Pure Genome Competitor 1 Competitor 2 70 60 % GC 40 30

Amplicon yield (µg/25-µl reaction)

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Figure 1.

A) KOD Polymerases (blue) yield more DNA in fewer PCR cycles than other high fidelity polymerases (other colors). B)  Efficient amplification of a 6.98 kb GC-rich gene from human genomic DNA. This gene (53% GC-rich overall) contains extremely GC-rich blocks: 150 bp (84% GC) and 529 bp (76% GC). C)  KOD polymerase limits GC library bias during Next Generation Sequencing (NGS) library preparation as compared to other commercially available products. Preparation performed using EMD Millipore’s PureGenome™ High Efficiency NGS Library Reagents. Visit www.emdmillipore.com/mobio for additional details.

23 25 27 29 Cycle number KOD Hot Start (1) KOD (1) Pyrococcus Polymerase Hot Start (Supplier I) (2) T. zilligii Polymerase (Supplier I) (2) Hot Start Polymerase (Supplier N) (3) Pfu Hot Start Polymerase (Supplier A) (2) Hot Start Polymerase (Supplier T) (1)

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10 0

EMD Millipore is a division of Merck KGaA, Darmstadt, Germany

Transfection Reagents: Healthy cells mean more discoveries.
Choose our proven transfection reagents, optimized for low toxicity and maximum protein expression. Browse our updated table of published transfection conditions! www.emdmillipore.com/transfection_citations
Transfection Reagent Cell Lines and Applications

GeneJuice® Reagent NovaCHOice® Kit 293-Free™ Reagent NanoJuice® Kit Insect GeneJuice® Reagent RiboJuice™ Reagent ProteoJuice™ Reagent

The standard for transfection in most cell lines in serum-containing or serum-free media. Efficient, scalable protein expression in suspension CHO lines Maximal output from HEK293 suspension cultures Overcome the most difficult-to-transfect mammalian cells (e.g. primary cell lines) Optimized for insect cells Effective delivery of siRNA for targeted gene silencing in most cell lines Delivery of even the most complex proteins into most cells

A. GeneJuice® reagent: Better efficiency than competitor products!
1000 900 800 SEAP Concentration (ng/ml) 700 600 500 400 300 200 100 0 GeneJuice® Reagent Reagent Reagent Reagent Reagent L2Ka F6 X9 JPE JP 24 hours 48 hours 72 hours β-Gal Expression (µg/mL)

B. NovaCHOice® kit: Protein yields in just 1 day
0.24 0.22 0.2 0.18 0.16 0.14 0.12 0.1 Transfection Reagent F NovaCHOice Transfection Kit NovaCHOice Transfection Kit with Booster

1

3 Days

5

7

Figure 1.
A)  Higher secreted alkaline phosphatase (SEAP) expression in HeLa cells using GeneJuice® Reagent than that using 5 other commercially available reagents at all time points. B)  Higher ß-Galactosidase expression in CHO-S cells at one day post transfection using the NovaCHOice® Transfection Kit compared to Transfection Reagent F.

To Place an Order or Receive Technical Assistance
In the U.S. and Canada, call toll-free 1-800-645-5476 For other countries across Europe and the world, please visit: www.emdmillipore.com/offices For Technical Service, please visit: www.emdmillipore.com/techservice

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EMD Millipore and the M logo are trademarks of Merck KGaA, Darmstadt, Germany. GeneJuice and NovaCHOice are registered trademarks and RiboJuice, 293-Free, ProteoJuice are trademarks of Merck KGaA, Darmstadt, Germany. PR5945EN00 BS-GEN-13-07950 03/2013 Printed in the USA. © 2013 EMD Millipore Corporation, Billerica, MA USA. All rights reserved.

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