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Sensoy et al.


Anim. Plant Sci. 21(4):2011 The Journal of Animal & Plant Sciences, 21(4): 2011, Page: J. 751-757 ISSN: 1018-7081

S. Sensoy, S. Demir*, S. Tufenkci**, Ç. Erdinç, E. Demirer*, H. Ünsal**, G. Halifeoglu, and A. Ekincialp Department of Horticulture, *Department of Plant Protection, **Department of Soil Science and Plant Nutrition, Agriculture Faculty, Yüzüncü Yıl University 65080 Van, TURKEY Corresponding author e-mail:

Four zucchini hybrids (Focus F1, Comet F1, Natali F1, and Ezra F1) inoculated by three different arbuscular mycorrhizal fungi (AMF) [Glomus intraradices (Gi), Glomus etinucatum (Ge), and Gigaspora margarita (Gm)] in a growth chamber experiment were evaluated for seedling traits, nutrient uptake, colonization, and relative mycorrhizal dependency (RMD). Relative mycorrhizal dependency ranged widely among four zucchini hybrids. Gm inoculations had higher positive RMDs, while Gi inoculations had lower negative RMDs. Gm-inoculated Focus F1 had the highest RMD (30.22 %). Arbuscular mycorrhizal fungi inoculated seedlings had wider cotyledons and stems. There were also significant effects of AMF inoculation on the most of nutrients. Key words: AMF, colonization, nutrient uptake, relative mycorrhizal dependency symbiosis, zucchini.

Arbuscular mycorrhizal fungi (AMF) are the most widespread root fungal symbionts and are associated with most plant species. Mycorrhizal symbiosis plays a significant role in the nutrition and development of host plants. Arbuscular mycorrhizal fungi (AMF) have been apparent to recover soil composition (Miller and Jastrow, 2000), and nutrient uptake of plants (Smith and Read 2008). Arbuscular mycorrhizal fungi (AMF) additionally support plants to cope with both biotic and abiotic stresses: they may fight with some soilborne pathogens (Garmendia et al. 2004; Hu et al. 2010), minimize some nutrient deficiencies (especially phosphorous and micro nutrients), advance aridity, salinity, and pollution tolerance (Turkmen, et al. 2005; Sensoy, et al. 2007; Turkmen, et al. 2008). Zucchini (Cucurbita pepo L.) is an important vegetable in Turkey and in the world (FAOSTAT 2009). So far, little research is done to reveal the effects of AMF on growth and yield of zucchini. Recent studies have suggested that AMF-inoculated zucchini could benefit from association with AMF (Colla et al., 2008; Cardarelli et al., 2010) by enhancing its nutrition in abiotic stress conditions. Differences in mycorrhizal responsiveness among diverse crops and between ranges of genotypes within the same crop have also been established (Sensoy et al., 2007; Meghvanski et al., 2008; Miyauchi et al. 2008; Wang et al. 2008; Long et al. 2010). Sensoy et al. (2007) detected that there was a large variation in mycorrhizal colonization dependency among the pepper genotypes, and confirmed that appropriate cultivar-AMF

combinations have to be found in order to obtain the greatest gain from symbiosis. Effectiveness of AMF is much better in a sterile growth medium (Wang et al., 2008). Inoculation with AMF, in some vegetables, may improve growth performance (Temperini et al., 2009). The benefits of AMF inoculation depend on the genotypic host-fungus combinations and also the type of the inoculums used (Rouphael et al. 2010). There has been insufficient attention to mycorrhizal response in the modern plant improvement activities, particularly in the development of hybrid varieties. Khalil et al. (1994) stated that modern cultivars in soybean showed lower growth response to mycorrhizal colonization than older cultivars. Even under normal seedlings conditions, the effectiveness of different AMF on different zucchini hybrids has not been well documented. Therefore, the current study initiated to evaluate colonization, nutrient uptake, responsiveness and some seedling traits of four zucchini hybrids inoculated by three different AMF.

Four zucchini hybrids Viz. Ezra F1 (Multitarim Seed Company), 2: Natali F1 (Anadolu Seed Company), 3: Focus F1 (Beta Seed Company), 4: Comet F1 (Beta Seed Company) used in protected cultivation in Turkey were examined. Three AMF inocula e.g. Glomus intraradices (Gi), Glomus etinucatum (Ge), and Gigaspora margarita (Gm) were used in the study. Inocula consisted of spores, extraradical mycelium and mycorrhizal roots. Growth medium comprised of an autoclaved mixture of perlite and peat moss (1:1 v/v) in seedling


21(4):2011 (Table 1). magnesium (Mg). 0. Ge-. Arbuscular mycorrhizal fungi applications had significant effects on seedling stem diameter. Plant Sci. Samples were then ovendried at 68 ºC for 48 h. Although the seedlings of Gi-inoculated Ezra F1. Zucchini roots were dyed to detect AMF presence using a modification of Phillips and Haymans (1970) method.2 mg KH2PO4.3 %. and root dry weights of seedlings were determined after harvesting. respectively. The lowest seedling emergence was observed in Gi-inoculated Ezra F1 followed by -AMF Ezra F1. The experiment was conducted using an 4×4 factorial design (four zucchini hybrids. 1. Seedling emergence percentage. but there were insignificant effects on leaf number. and nitrogen (N) contents of shoots measured using Kjeldahl method. 2007). 8. seedling emergence time. Gm-inoculated zucchini seedlings had the highest root fresh and dry weight followed by Ge-inoculated ones.70 % among the treatments. leaf numbers. Anim.5 % wider cotyledons than those of non-inoculated seedlings. and –AMF Natali F1 had the shortest cotyledons. Relative mycorrhizal dependency (RMD) of zucchini hybrids was expressed as the difference between the total dry weight of the mycorrhizal plant and the total dry weight of the non-mycorrhizal plant as a percentage of the total dry weight of the mycorrhizal plant (Sensoy et al. Data were analyzed using the SAS statistical program. Differences between treatments were determined using Duncan’s Multiple Range Test (SAS Software 1997). Ge-inoculated Natali F1. and Gminoculated seedlings had 8. and 0.5 cm W x 5. Hypocotyledon height.5 cm L x 5. and manganese (Mn) contents of shoots measured using atomic absorption spectrophotometer . phosphorous (P) contents of shoots measured using the vanadate-molybdate-yellow procedure with spectrophotometer (Kacar and Inal. root lengths. Gm-inoculated ones had the lowest P content in shoots. Gi-. Giinoculated seedlings had the lowest shoot fresh and dry weight among the applications. 0. J. Shoot heights. While Gi-inoculated zucchini seedlings had the highest P content in shoots. 2008). and then Gi-inoculated ones. 295 mg Ca(NO3)2.75 mg MnCl2. However. and stem diameter were measured.1%. potassium (K). Gi-. and Giinoculated seedlings had 7. On the other hand. One seed was sown per cell. 1999). there was significant mycorrhizal effect on iron (Fe) and Zinc (Zn) contents and mycorrhizal colonization (Table 5). respectively. cotyledon height.3 mg FeNaEDTA. and root length (Table 2). Ge-. ground. Ge-inoculated ones had the lowest Fe and Zn contents. and irrigated with distilled water.40 % to 46. calcium (Ca).000 lux light intensity). 16. for a total of 720 cells.5H2O. shoot dry weights. zinc (Zn).2H2O) modified by Vosatka and Gryndler (1999).. 0. there was significant genotypic effect on phosphorous (P). Arbuscular mycorrhizal fungi applications had no significant effects on fresh and dry weights of zucchini seedling shoots and roots (Table 3). RESULTS Arbuscular mycorrhizal fungi application and arbuscular mycorrhizal fungi-zucchini hybrid interaction had significant effects on seedling emergence percentage 752 . Gi-inoculated zucchini seedlings had the highest K content in shoots compared to the other applications. Gm-. with variance analysis conducted for all data. and Mg contents Although AMF applications had no significant effect on copper (Cu) and manganese (Mn) contents. While Gi-inoculated zucchini seedlings had the highest Fe and Zn contents. Seedling trays were placed in a growth chamber at a temperature of 222oC with 12 h fluorescent illumination (8.3% wider stem diameters than those of non-inoculated seedlings. and true leaf emergence time were determined. Mycorrhizal colonization ranged from 24.00017 mg Na2MoO4. followed by Gi-inoculated ones. the seedlings of Geinoculated Comet F1 had the longest cotyledons (Table1). iron (Fe).75 mg ZnSO2. potassium (K) and calcium (Ca) contents (Table 4).1 % shorter hypocotyledons than those of non-inoculated seedlings. and the percentage and intensity of mycorrhizal colonization was estimated using the gridline intersect method (Giovanetti and Mosse 1980). each of which contained 80 cm3 of sterilized growth medium.H2O.75 mg KI. stem diameters. Ca. There were also significant zucchini hybrid × AMF interactions on P. Plants were harvested 9 weeks after seed sowing and inoculation. and Gm-inoculated seedlings had 6. there were no significant effects of AMF inoculation on seedling emergence time (Table 1). cupper (Cu).5 mg H3BO3. but wider cotyledons than those of non-inoculated seedlings.2 %. Hypocotyledon height and cotyledon height were significantly affected by genotype and AMF (Table 1).4H2O. five g (25 spores g-1) of inoculums were placed in the growth medium before the seeds were sown (Demir and Onogur. Each seedling was fertilized twice with a 5 ml of nutrient solution (for 1 L: 720 mg MgSO4 7. Arbuscular mycorrhizal fungi inoculated seedlings had shorter hypocotyledons. Although AMF applications had no significant effects on nitrogen (N) and magnesium (Mg) contents. 4. and 16. trays (Each individual plant cell measures approximately 4. In the AMF-inoculated samples.4H2O. shoot height.1 %.H2O.001 mg CuSO4. 7. Similarly.9 %. 12. K. three AMF plus one control) with three random replications of fifteen cells each. On the other hand.2%. respectively. Gm-inoculated zucchini seedlings had the highest Ca content in shoots compared to the other applications. cotyledon length. and 4. 0.5 cm D) covered by vermiculite. and 10. Ge-. 240 mg KNO3.Sensoy et al. Ge-and Gm-inoculated zucchini seedlings had the maximum stem diameter.

which might be due to a better nutritional status. Wang et al. (2010) stated that the major fluxes in AMF symbiosis appear to be of carbon from plant to fungus and phosphorous.22 %). There are many other benefits of AMF in agriculture. Anim. Plant Sci. 2008. +AM plants had also higher cotyledon. (2008) studied the effects of AMF on salt stressed zucchini plants grown at low and high phosphorous concentration. (2008) reported that the growth of cucumber seedlings was also influenced differently by the studied three AMF species. These researchers demonstrated that +AM plants under alkaline conditions had higher total. Temperini et al. intraradicies. (2008) speculated that growth response induced by a specific AMF relied on recognition between AMF species and host plant. The limited number of studies on the effects of AMF in zucchini has suggested that AMF-inoculated zucchini could benefit from association with AMF in abiotic stress conditions (Colla et al. Gm-inoculated Focus F1 had the highest RMD (30. DISCUSSION Many crops benefit from AMF in a variety of biotic and abiotic stress conditions... These researchers stated that the beneficial effects of AM on zucchini plants could be due to an improvement in water and nutritional status (high K and low Na accumulation). Long et al. sometimes negative growth response by AMF induced by the imbalance of carbohydrate distribution between the shoots and roots of the plants.. Their results indicated that growth of seedlings was significantly enhanced by G.. Azcon et al. marketable yield and total biomass compared to –AM plants. Figure 1. Fe. and the development of cultivars with improved symbiotic qualities would insure the production of good crop yields while improving agrosysytem sustainability (Hamel and Strullu 2006). 753 .22% to -30. 2010). from fungus to plant. Natali. There are variations in carbon demands of the different AMF species (Saikkonen et al. and Zn and lower Na accumulation. Hodge et al. Wang et al. (2008) also found that N and P contents in cucumber roots and Mg. Cu.Sensoy et al. Relative mycorrhizal dependency (RMD) ranged widely among the zucchini hybrids tested (Figure 1). cotyledon width and stem diameter were detected. Miyauchi et al. and Zn). Gi-inoculations had all negative RMDs on all hybrids. mossae. K. and not significantly influenced by G. (2009) stated that AMF inoculation showed improvement in transplant performance in local pepper genotypes. (2010) studied the effects of AMF on alkaline stressed zucchini plants grown under mineral and organic fertilization. Relative mycorrhizal dependency (RMD) of four zucchini hybrids (Ezra. Cardarelli et al. 1999). K. Cardarelli et al. 2008.. In the present study. Cadradelli et al. even in phosphorousrich soils. even the root colonization was similar. Colla et al. 2008. Gminoculated Focus F1 had the highest RMD (30. 2008. and Gigaspora margarita (Gm)]. Wang et al. stem diameter.. Focus. Ca. and root fresh weight compared to –AM plants. J. AMF may positively affect crop development.22 %). the significant effects of AMF inoculation on the most of nutrients (especially on P. (2010) also demonstrated that +AM zucchini plants had higher P. and possibly nitrogen. but several studies have shown that genetic variation in responses to AMF is obvious regardless of the crop (Sensoy et al. 21(4):2011 Relative mycorrhizal dependency (RMD) ranged widely (30. (1991) stated that there were differences in functional compatibility among AMF. On the other hand. and Mn contents. Zn contents in cucumber shoots were increased by inoculating AMF. Gminoculations had positive RMDs on all hybrids. Gm inoculations had higher positive RMDs. and Comet) inoculated with three AMF [Glomus intraradices (Gi ). Meghvanski et al. (2007) that compared different pepper genotypes and AMF. Glomus etinucatum (Ge).. Zn. 2010). Mn.. inhibited by G.19%) among four zucchini hybrids with no clear correlation between mycorrhizal response and AMF colonization level and intensity. The finding of a lack of correlation between AMF colonization and RMD was similar to the findings of a study by Sensoy et al. while Gi inoculations had lower negative RMDs.. there has been no study on zucchini hybrid-AMF interaction. Wang et al. Fe. veriforme. There were also significant zucchini hybrid × AMF interactions on Fe. However. Geinoculations had also positive RMDs except the Natali F1. 2007.

62 b 33.90 def** 52.89 52.61 3.00 a 96.30 f 47. hypocotyledon height.00 a 98.03 12.inoculated Ezra F1 Ge.inoculated Ezra F1 Non inoculated Natali F1 Gm.92 6.89 Leaf number 4..25 12.64 6.87 a-e 44. Effects of AMF [Glomus intraradices (Gi ).inoculated Ezra F1 Non inoculated Natali F1 Gm.inoculated Ezra F1 Gi.45 c** 3.37 b 70.07 5.58 19.00 a 100.14 11.53 abc 62.50 37.60 NS 23. and Gigaspora margarita (Gm)] and genotype on seedling stem diameter.00 a 100. NS: nonsignificant.73 a*** 4.75 NS 4.59 3.89 5.83 32.63 71.21 a 21.67 b* 21.81 6.43 11.33 73.77 a 3.37 5.01.77 4.inoculated Comet F1 Stem diameter (mm) 3.83 3.67 74. AMF: Arbuscular Mycorrhizal Fungi.80 31.59 23.68 a 30.03 21.50 4.99 12.75 3.26 3.81 21.inoculated Ezra F1 Gi.32 NS 54. 00 a 100.73 12.68 3. ***:P<0.inoculated Focus F1 Ge.75 a 51.89 a 4.19 Values are the means of three replicate samples. *:P<0.37 c-f 47.98 a 66.80 29. –AMF: Nonmycorrhizal plants.08 13.68 20.43 3.07 4.57 NS 35.61 4.63 75.29 NS 4. and root length of zucchini plants.63 c-f 52.27 f 52.25 b 6.73 4.inoculated Natali F1 Gi. 00 a 100. Anim.50 bc 3.inoculated Focus F1 Non inoculated Comet F1 Gm.70 69.26 b 51.55 21.25 12.53 c-f 46.06 NS 6.49 3.38 5.001(significant) Table 2.81 a 3. cotyledon height.00 a Seedling emergence time (day) 7.10 NS 64.02 NS 13.00 a 100.inoculated Comet F1 Ge. 00 a 100. and cotyledon length of zucchini plants.90 28.14 8.60 14.31 NS 12.60 13.23 21.63 66. Applications Ezra F1 Natali F1 Focus F1 Comet F1 -AMF AMF Gm Gi Ge Non inoculated Ezra F1 Gm.76 19. 21(4):2011 Table 1.80 a-d 65.49 Shoot height (cm) 13.44 b* 3. –AMF: Nonmycorrhizal plants.00 a 95. seedling emergence time.70 4.50 b** 100.001(significant) 754 .54 ab 74.97 5.73 3.98 21.inoculated Comet F1 Hybrids Seedling emergence (%) 96.17 36.00 a 100.83 def 57.22 12.61 49. Glomus etinucatum (Ge).inoculated Natali F1 Non inoculated Focus F1 Gm.99 5.25 Root length (cm) 21. J.inoculated Natali F1 Gi.87 13. 00 a 100.72 3.52 b*** 49.04 b 24. **:P<0.02 6.56 b 6.43 29.55 4.76 4. shoot height.09 NS 3.67 b 4.89 b 34.67 6.67 b 100.76 NS 12.65 Cotyledon length (mm) 50.inoculated Focus F1 Non inoculated Comet F1 Gm. NS: nonsignificant.23 71.80 73.55 b 61.96 21. 00 a 100.94 a 29.40 24.90 67. ***:P<0.72 Hypocotyledon height (mm) NS 8.63 5.52 4.03 NS 22. **:P<0. Effects of AMF [Glomus intraradices (Gi ).71 21.95 Cotyledon width (mm) 33.59 ab 3. *:P<0.07 b-f 51.inoculated Natali F1 Ge.60 61.70 3. AMF: Arbuscular Mycorrhizal Fungi.inoculated Focus F1 Ge.inoculated Comet F1 Gi.41 3.19 13.inoculated Natali F1 Non inoculated Focus F1 Gm.61 3. 00 a 100.03 13. leaf number.04 23.69 a 3.03 f 48.95 a* 67.94 3.31 12.75 a*** 5.70 3. Glomus etinucatum (Ge).22 7.55 10.20 33.73 28.44 b 70.53 11.inoculated Focus F1 Gi.80 a 31.02 c*** 33. 00 a 100.05 a Values are the means of three replicate samples.67 4.inoculated Focus F1 Gi.59 6.00 a 100.83 ab* 100.23 22.33 ab* 100.03 3.00 a 90. Applications Ezra F1 Hybrids Natali F1 Focus F1 Comet F1 -AMF AMF Gm Gi Ge Non inoculated Ezra F1 Gm.inoculated Comet F1 Gi.89 b 21.13 24.94 26.80 80.37 3.64 ab* 74.69 a 3.67 b 100.33 32.57 12.inoculated Natali F1 Ge.81 ab 3.70 ab 58.38 ab 68.inoculated Ezra F1 Ge.65 6.05 13. 00 a 100.00 a 100.00 35. and Gigaspora margarita (Gm)] and genotype on seedling emergence percentage.46 4.39 13.10 66.37 b 30.37 21.03 36. Plant Sci.70 b-f 61.49 53.75 b 78.37 25.13 30.inoculated Comet F1 Ge.Sensoy et al.80 ef 54.96 6.00 61.77 69.96 4.03 a*** 29.91 6.72 7.89 b 6.

63 353 25.96 2. calcium (Ca).20 bcd 1.47 jk 3.19 *** 4.29 a 1.67 a Gi 4.25 b 1.75 bc*** 1.71 a 442 a 30.99 ab Gi.43 k 3.62 a-d 1.53 b*** 1.16 bcd 1. and magnesium (Mg) contents of shoots.36 a-d 0.81 bc 1.76 Ge.57 3.19 Gi.02 ab 0.40 b 0.68 -AMF 2.93 0.70 367 29.15 b 4.28 ab Gi.73 h 2.inoculated Comet F1 1.20 ab Gm.52 NS AMF Gm 2.85 a 2.79 417 33.76 410 31.47 Focus F1 1.51 0.60 b 1.001(significant) 755 .17 0.49 a-d 3.73 0.inoculated Ezra F1 5.08 0. NS: nonsignificant.35 0.54 Ge 2.82 b 1.inoculated Natali F1 2. Shoot fresh weight Root fresh Shoot dry weight (mg Root dry weight (mg (g plant-1) weight (g plant-1) plant-1) plant-1) Ezra F1 5. Applications Nitrogen (%) Phosphorous (%) Potassium (%) Calcium (%) Magnesium (%) Ezra F1 3.71 0.67 ab 379 b 28.inoculated Focus F1 6.55 b 1. 21(4):2011 Table 3. J.46 Gi 2.28 ab Values are the means of three replicate samples.49 c** 370 b** 19.49 343 21.21 0.28 b 1.18 0.07 a Non inoculated Ezra F1 5.98 Focus F1 6.53 NS 0.34 a 0. Glomus etinucatum (Ge).77 b 1.65 383 26.52 1. Effects of AMF [Glomus intraradices (Gi ).53 NS 331 NS 21.22 ab Ge.29 0.29 Gm.92 d 4.66 NS 1.inoculated Natali F1 2.27 e 4.90 f 3.25 0.35 a 4.17 a *** 2.77 c 2.96 1.62 277 21. AMF: Arbuscular Mycorrhizal Fungi. –AMF: Nonmycorrhizal plants. Anim.11 1.09 ab Non inoculated Focus F1 1.Sensoy et al.28 Non inoculated Focus F1 6.36 b*** AMF Gm 6.39 NS 337 NS 15.inoculated Focus F1 5.49 ab Gm.66 c 1.87 a 0.59 368 24.56 377 22.14 b** Gm..55 367 23.27 ab 3.43 0.001(significant) Applications Table 4. potassium (K).33 bc 1.80 g 2.01.08 bc 1.20 NS Hybrids Natali F1 2.72 Gi.inoculated Ezra F1 4.30 NS Gm.inoculated Comet F1 6. **:P<0.19 -AMF 6.inoculated Comet F1 5.inoculated Focus F1 1.47 jk 3.36 bc 1.75 537 35.53 j 2.51 Non inoculated Ezra F1 2.88 d 2.49 b 0.33 0.01.19 c** 0.07 bc *** 3.46 360 19.28 b 3.55 407 20.43 ab Gi.inoculated Natali F1 3.56 Non inoculated Natali F1 5.63 i 3.74 0.70 Ge.00 bcd 0.03 0.21 Gm. and Gigaspora margarita (Gm)] and zucchini hybrids on nitrogen (N).67 b 1.22 0.40 0.58 a 0.07 0. AMF: Arbuscular Mycorrhizal Fungi.31 0.38 0.87 a*** 1.61 Gm.22 ab Ge.56 bc 283 c 20.inoculated Focus F1 1.62 397 25.inoculated Natali F1 4.38 b 1.22 0.14 1.75 c 4.17 3.inoculated Ezra F1 3.25 NS 1.30 1.85 3. **:P<0.inoculated Ezra F1 5.13 ab Gm.13 b Gi.64 392 26.39 a-d 2.27 a 2. and Gigaspora margarita (Gm)] and zucchini hybrids on fresh and dry weights of shoots and roots.97 b*** 1.21 Comet F1 6.59 0.31 a-d 8.67 hi 2.92 a 0.inoculated Comet F1 1.54 ab* 2.89 c 3.77 c 4.54 a Ge.31 b 2.inoculated Ezra F1 3. –AMF: Nonmycorrhizal plants.13 bc 1.63 257 21.39 a-d 0.inoculated Natali F1 6.07 b Ge.07 3.63 d 3.11 0.inoculated Focus F1 6.30 a 3. ***:P<0.41 ab Non inoculated Natali F1 3.58 Non inoculated Comet F1 7.33 bc 1.62 Comet F1 1.48 253 18.38 Gi.03 Ge.57 NS Hybrids Natali F1 5. Glomus etinucatum (Ge).37 Gi.inoculated Focus F1 1.inoculated Comet F1 1.inoculated Natali F1 5.74 bc 1.43 a-d 3. ***:P<0.47 bc 0.35 bc 1.inoculated Comet F1 7.inoculated Ezra F1 2.98 bc 1.04 a** 0. NS: nonsignificant. Effects of AMF [Glomus intraradices (Gi ).99 bcd 2.77 c*** 4.48 b 1.90 1.64 a 1.73 450 30.51 0.04 b 2. phosphorous (P). Plant Sci.13 a** 3.13 b Non inoculated Comet F1 1.86 b Ge 5.83 Values are the means of three replicate samples.29 0.60 c 1.93 cd 1.94 Ge.87 bc 2.

79 b 214.inoculated Natali F1 57.00 de 24. New Phytol. NS: nonsignificant.50 d 187.84 b 26. and E.01 ab*** 203.47 bc 46.17 d 194. Gminoculated Focus F1 had the highest RMD (30.91 51.29 40.76 b 195.35 abc 24.47 c 24.inoculated Natali F1 189. and Gigaspora margarita (Gm)] and zucchini hybrids on mycorrhizal colonization and iron (Fe).00 b*** AMF Gm 94.56 a*** 26. Cardarelli.66 a Non inoculated Ezra F1 160. AMF: Arbuscular Mycorrhizal Fungi. Greater interest in this area.67 Non inoculated Focus F1 21.91 -AMF 86. Considering the wide variety of responses from different plant cultivars to AMFs. Rivera.76 NS 24.12 ab 155.17 bcd 184. S. 117:399–404. Rouphael.17 27. Effects of AMF[Glomus intraradices (Gi ).06 a Ge 56. Manganese (mg Mycorrhizal kg-1) colonization (%) Ezra F1 148. Anim.00 29. as demonstrated in this and other studies.67 NS 0.67 cde 18. Onogur (1999). N 2-fixation (15N) and nutrition of Medicago sativa L.66 23. and G. Phytopatol.27 Ge.39 42.50 de 22.17 de 20.inoculated Natali F1 76. FAOSTAT. Among the examined AMF. Rea (2008).25 d 188.50 e 22.67 28.inoculated Focus F1 39.00 Gm.89 c 20.10 c 19. J.61 a Gi 175.00 Gm. Alleviation of salt stress by arbuscular mycorrhizal in zucchini plants grown at low and high phosphorus concentration. The determining the best AMF-cultivar combinations with improved symbiotic qualities would insure the production of good crop yields while improving agro-system sustainability. AMF may enhance seedling traits in vegetable species. M.00 36. Barea (1991). could lead to improvements in zucchini production by increasing strength under various biotic and abiotic stress conditions that might be encountered during the crop production season. Colla.00 cde 21. Biol. 173: 778–787..inoculated Comet F1 126..inoculated Ezra F1 213. but effectiveness of AMF on full growing period of hybrids should also be investigated in depth for protected cultivation conditions in the future. C.70 Ge. incorporating AMF-related traits from highRMD genotypes into modern cultivars.33 47.85 82. Growth response at seedling nursing could be a good indicator of AMF applications.01 ab 33.49 bc 37. Plant Nutrition & Soil Sci. REFERENCES Azcon. copper (Cu).50 35.03 a 194.27 abc 195.99 93.33 abc 188.60 Non inoculated Comet F1 38.92 a 31. Applications Iron (mg kg-1) Copper (mg kg-1) Zinc (mg kg-1) In conclusion. As shown in the examples.46 57. Gigaspora margarita followed by Glomus etinucatum (Gm) gave better RMDs for zucchini hybrids. G.80 NS Hybrids Natali F1 108.25 76.29 89.50 bc 0. M. Rouphael.33 bc 35.40 Gi.. Turk.M. So far.90 a 228. 44(3):501-509. Plant Sci. and E. Demir.94 29.61 65.67 ab 40.50 bc 44. R.75 de 14.29 ab 30.29 31.89 94.50 e 12. and J.02 a 756 . http://apps.46 b 22. AMF offer valuable opportunities for the existing agricultural practice with regard to various biotic and abiotic stress conditions.00 Gm.14 a*** 204. (2009) Statistic Database.83 cd 248.33 a 36.40 abc** 0.21 Comet F1 69. Y.50 39. ***:P<0.00 bc 30.00 NS Gm. Tullio.17 d 200.19 ab 34.87 Gi.17 51.868 a*** 188.50 bcd 21.01 a 201. modern plant breeding methods have paid little attention to such symbiotic relationships.25 41.72 NS 70.83 bc 46. and their effects on growth.95 abc*** 27. –AMF: Nonmycorrhizal plants.24 30. **:P<0.51 Focus F1 89. zinc (Zn).001(significant).. Y.39 48.inoculated Comet F1 37.11 Gi.inoculated Focus F1 225.inoculated Comet F1 74.. R.15 Values are the means of three replicate samples.. 21(4):2011 Table 5. Colla (2010).42 72.50 d 217. Cardarelli. Selective interactions between different species of mycorrhizal fungi and Rhizobium meliloti strains. Glomus intraradices Schenck&Smith: A hopeful vesicular-arbuscular mycorrhizal (VAM) fungus determined in soils of Türkiye.00 bc 0.fao.06 NS 89.inoculated Focus F1 27. In the present study.25 de 27.59 bc*** 25. J.33 bc 22. Rea.00 cd 193. M.01.inoculated Ezra F1 67. E.05 NS 65.50 cde 28.. Glomus etinucatum (Ge). & Fertility of Soils.88 c 199.79 ab 0.75 bc 39.83 d 186.67 bc 37.inoculated Ezra F1 153. M. Mitigation of alkaline stress by arbuscular mycorrhiza in zucchini plants grown under mineral and organic fertilization. and manganese (Mn) contents contents of shoots.48 74.22 %). Effective use of these symbiotic soil fungi is an essential element for sustainable agriculture. Rubio. appropriate cultivar-AMF combinations need to be identified in order to derive the utmost benefit from symbiosis.70 Non inoculated Natali F1 88.99 b 200.00 46.07 Gi. 28:33-34. J.17 Ge.10 Ge.72 a 28.79 a 197.Sensoy et al.

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